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<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTE5</accession>
    <name>N6UTE5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Group 1 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_07137</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000075">
        <property type="protein sequence ID" value="ENN95569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004595030.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTE5"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="2"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="34" mass="3646" checksum="0F9C6E8AC259BD72" modified="2013-06-26" version="1">MLIEANACYKPVIAYKSGGVVEVVDNGFNGFLVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTG9</accession>
    <name>N6UTG9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11 12">Threonine synthase</fullName>
            <ecNumber evidence="4 11 12">4.2.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">J422_06947</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the gamma-elimination of phosphate from L-phosphohomoserine and the beta-addition of water to produce L-threonine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>O-phospho-L-homoserine + H2O = L-threonine + phosphate</text>
            <dbReference type="Rhea" id="RHEA:10840"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="EC" id="4.2.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12 13">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the threonine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.1" evidence="4 11 12"/>
    <dbReference type="EMBL" id="APMM01000065">
        <property type="protein sequence ID" value="ENN95604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594633.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTG9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00856"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1346"/>
    <dbReference type="OrthoDB" id="6371at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00065"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003941">
        <property type="term" value="F:L-serine ammonia-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004794">
        <property type="term" value="F:threonine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004795">
        <property type="term" value="F:threonine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006565">
        <property type="term" value="P:L-serine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006567">
        <property type="term" value="P:L-threonine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01563">
        <property type="entry name" value="Thr-synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000013">
        <property type="entry name" value="Threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000014">
        <property type="entry name" value="Threonine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050147">
        <property type="entry name" value="Ser/Thr_Dehydratase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000634">
        <property type="entry name" value="Ser/Thr_deHydtase_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004450">
        <property type="entry name" value="Thr_synthase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026260">
        <property type="entry name" value="Thr_Synthase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006050">
        <property type="entry name" value="PRK08197.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00260">
        <property type="entry name" value="thrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078:SF6">
        <property type="entry name" value="L-THREONINE DEHYDRATASE CATABOLIC TDCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48078">
        <property type="entry name" value="THREONINE DEHYDRATASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038945">
        <property type="entry name" value="Thr_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00165">
        <property type="entry name" value="DEHYDRATASE_SER_THR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12 17" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12 13" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="16">
        <location>
            <begin position="68"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="233"/>
            <end position="237"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="371"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="14">
        <location>
            <position position="106"/>
        </location>
    </feature>
    <feature type="cross-link" description="Isoglutamyl lysine isopeptide (Lys-Gln) (interchain with Q-Cter in protein Pup)" evidence="15">
        <location>
            <position position="188"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="NCBIfam" id="TIGR00260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR038945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR038945-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN95604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="405" mass="44943" checksum="BD7B1682A401B5A5" modified="2013-06-26" version="1">MLQRCIECGKTYKKEDIVYTCECGGLLEVVYDYEEIKEKISKETFKKREPGVWRYIEFLPIEDMSKIISLQEGNTPLYRCKNLEKELNVKELYVKNEGANPTGSFKDRGMTVGVSVANELNIKSVGCASTGNTSASLAAYSAKANMKCIVLLPEGKVALGKLAQAMFYGAKVIQIRGNFDDALEIVKKLADERLIYLLNSVNPFRLEGQKTIAFEICERLNYNVPDRVIVPVGNAGNISAIWKGFKEFYITGLIDELPKMTGIQAEGAKPIVEAFKKKKLEIEPYKNPETVATAIRIGNPVNAKKALKAIYDSNGYAEAVSDREIIEAQKLLARKEGIFVEPASASSIAGLKKLLEEGVIDRDERIVCITTGHGLKDPDIAIRESEEPIKVEADLEKIKSLLMEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTI5</accession>
    <name>N6UTI5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">C/D box methylation guide ribonucleoprotein complex aNOP56 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06902</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTI5"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1339"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.246.90:FF:000007">
        <property type="entry name" value="Pre mRNA splicing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.4070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.246.90">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042239">
        <property type="entry name" value="Nop_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51358">
        <property type="entry name" value="NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="Nop" evidence="2">
        <location>
            <begin position="50"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="163"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="1">
        <location>
            <begin position="165"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="194" mass="22186" checksum="C7563A96DD0E3C7B" modified="2013-06-26" version="1" fragment="single">KIAENAKSSMGGEFEDYDLEAVVKFAEEISHLYEKRKDLYEYLEKLMKEEAPNLTKLAGVSLGAKLICLAGGLEKLAKLPASTIQVLGAEKALFAHLRLKVDPPKHGVIYNHPLIKNAPKWQRGKIARALACKLAIAARADYLGDDIADELYEKLMKRVEEIRKKYPKPPKKKKVKKKFKKKKEKRFKKKREKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTJ9</accession>
    <name>N6UTJ9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">DNA polymerase II large subunit</fullName>
            <shortName evidence="16">Pol II</shortName>
            <ecNumber evidence="16">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Exodeoxyribonuclease large subunit</fullName>
            <ecNumber evidence="16">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">polC</name>
        <name evidence="19" type="ORF">J422_06663</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 16">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 21">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 16">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 16">Belongs to the archaeal DNA polymerase II family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="16"/>
    <dbReference type="EC" id="3.1.11.1" evidence="16"/>
    <dbReference type="EMBL" id="APMM01000057">
        <property type="protein sequence ID" value="ENN95649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="STRING" id="1069083.GCA_000371805_00718"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1297"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00081">
        <property type="entry name" value="Hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.16.10">
        <property type="entry name" value="Hedgehog/Intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00324">
        <property type="entry name" value="DNApol_II_L_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004475">
        <property type="entry name" value="PolC_DP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056172">
        <property type="entry name" value="PolC_DP2_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056171">
        <property type="entry name" value="PolC_DP2_central_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016033">
        <property type="entry name" value="PolC_DP2_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00354">
        <property type="entry name" value="polC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003103">
        <property type="entry name" value="PRK04023.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011303">
        <property type="entry name" value="PRK14715.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42210">
        <property type="entry name" value="DNA POLYMERASE II LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42210:SF1">
        <property type="entry name" value="DNA POLYMERASE II LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14890">
        <property type="entry name" value="Intein_splicing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24846">
        <property type="entry name" value="PolC_DP2_cat"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24844">
        <property type="entry name" value="PolC_DP2_central"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03833">
        <property type="entry name" value="PolC_DP2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="Hedgehog/intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="17" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 16" id="KW-0235">DNA replication</keyword>
    <keyword evidence="12 16" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 16" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9 16" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="7 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13 16" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="6 16" id="KW-0540">Nuclease</keyword>
    <keyword evidence="4 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="18">
        <location>
            <begin position="1078"/>
            <end position="1148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="17">
        <location>
            <begin position="1322"/>
            <end position="1349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ENN95649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="1462" mass="168558" checksum="BEE67F7ED1896E3E" modified="2013-06-26" version="1">MVECSERMKKYFDNIVKEVEKIYRIAEECRKLGLDPCDYVEIPLATDMAGRVEGLVGPKGVAERIRELVKEFGKEEAALKIAEEIIEGKFGKFDREKAAEQAVRTALAVLTEGIVAAPLEGIADVKIKKNHDGSEYLAIYYAGPIRSSGGTAQALSVLVGDFVRKALKLDRYKPTEDEIERYVEEVELYLSEVGSFQYTPTADEIRKAVRNIPIEITGEATDDVEVSGHRDLERVETNQLRGGALLVLVEGVLLKAPKLLKYIDKLNVEGWDWLKEFVNKEEEKEEEDEEEIEDEDINLKYWRDVNIEANKKYISEVIAGRPVFSHPSRIGGFRLRYGRARNTGFAAQGFHPALMYLVDEFIAIGTQLKTERPGKGTCAMPVDSIEGPIVRLKNGDVIRVETVEQALNVRDKVDKILFLGDVLVNYGDFLENNHPLIPSCWCEEWFEEILKSKGIKYDREFIKNPKPEDAVKFALETKTPLHPRFTYRWHDVSKEDIIKLRKWLLKGEEGYYKNEKVWIVEKSKEKEILENIGCPHLVRDNKIIIREYYPLLYSLGYDVENKRDLFNEDDVKNAKNSMHLINIMAPFEVRRNTYVYVGARMGRPEKAAPRKMKPPVNGLFPIGNAGGLVRLINKAVEENNTDSIEVSYGICKCGKISLYRVCPYCGRRVELNRYGKIVAPLKDYWFMALKRLKINKPGDVKCIKGMTSKKKIVEPLEKAILRAMHEVYVFKDGTARFDTTDVPITHFKPKEINVSVEKLKELGYDRDIYGNPLTSEDQILELKPQDIIIPEACAEYFVRVANFIDDLLEKFYNKERFYNIKKKEDLIGHLVIGLAPHTSAGMVGRIIGYVKANVCYAHPYFHAAKRRNCFTKDTRVLVNIDGDVKRVTFEELYNLFEDESYENFAYIRKKPKKDIKVYSFDIKNKKVVLTEIEEVIKAKSPNHLLKFILETGREFVTTHDHPVLVYKDNKFVEKKAIEVEEGDLILIPKIEFQEDDIKEIDLLEKLLKEEFKDLWEMVEIEGLEKWLNINKKKISLSELLKILSDYNLSIADVPKDCYLIIKDEKIKRVVDIESLLKIIGHIVKGDKSNLILRRLFKIEEGRIPSFIFKLPKERIKLFISTLFSNGRIKCKNKMLLEDIDLLLNRLGIISSWEDGELILTDKVKKLNFGWVLAVKKIEPIVSENEYIYSLNAKEHHNIIINSNINVHQCDGDEDSLFLLLDAFLNFSKLFLPDKRGGQMDAPLVLTTILDPKEVDGEVHNMDVMWRYPLEFYERTLNMPSPKELRDIMEVVEDRLGKPEQYENLGYTHETSSIDLGPKLCAYKTLNTMLEKTESQLKVAEKIRATDERDVAEKVIQSHFIPDLIGNLRAFSRQGVRCKCGAKFRRVPLRGKCPKCGSNLILTVSKGAVEKYMDVAEKMAEKYNVNNYIKQRLKLIREGIKSIFENDKNKQIKLIDFFNNYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTM5</accession>
    <name>N6UTM5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">DNA primase small subunit PriS</fullName>
            <ecNumber evidence="11">2.7.7.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">priS</name>
        <name evidence="14" type="ORF">J422_06326</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalytic subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. The small subunit contains the primase catalytic core and has DNA synthesis activity on its own. Binding to the large subunit stabilizes and modulates the activity, increasing the rate of DNA synthesis while decreasing the length of the DNA fragments, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="function">
        <text evidence="13">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="11">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11 12">Belongs to the eukaryotic-type primase small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.-" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593502.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTM5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01308"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1231"/>
    <dbReference type="OrthoDB" id="31125at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04860">
        <property type="entry name" value="AE_Prim_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.920.10">
        <property type="entry name" value="DNA primase, PRIM domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00700">
        <property type="entry name" value="DNA_primase_sml_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002755">
        <property type="entry name" value="DNA_primase_S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014052">
        <property type="entry name" value="DNA_primase_ssu_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023639">
        <property type="entry name" value="DNA_primase_ssu_PriS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00335">
        <property type="entry name" value="primase_sml"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10536">
        <property type="entry name" value="DNA PRIMASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01896">
        <property type="entry name" value="DNA_primase_S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56747">
        <property type="entry name" value="Prim-pol domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="2 11 12" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10 11" id="KW-0464">Manganese</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3 11 12" id="KW-0639">Primosome</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 11 12" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="11">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009762"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN95684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="329" mass="39817" checksum="DCE45E1E99228070" modified="2013-06-26" version="1">MEVVKRLYKEYYNYVINNNLLEVPEKIEYREFGYGYLKKVDNRNLSFKNEKEYKMWVLSNTPFHLYKSLAYCYYPNRQGGAKQKEVFRRELAFDIDVHKTEKCKHDNDFICKYCLEKAKKQALYLIDEFLLTDFGLKEDDILIVFSGNRGFHIYIKPKDLKLRDIIEQYSKEERRFLLSYVLGEHLNPSYIGYGWRRRLFKLLKPKDKKLLYRKNWKGFFNKDVILKNRLELDEKVMEDDIRLLRVIYSLHGYTGFIVKPVAYKDLKKFNPLKDAIFGKFEEKWYKVEINSDREFSITINNRRYTNRSKKVSGTALLFLFGHNIKFELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTR5</accession>
    <name>N6UTR5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cation diffusion facilitator family transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_06264</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the cation diffusion facilitator (CDF) transporter (TC 2.A.4) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593355.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTR5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00286"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1220"/>
    <dbReference type="OrthoDB" id="8907at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1510.10:FF:000006">
        <property type="entry name" value="Divalent cation efflux transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1510.10">
        <property type="entry name" value="Cation efflux protein transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1350">
        <property type="entry name" value="Cation efflux protein, cytoplasmic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002524">
        <property type="entry name" value="Cation_efflux"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027470">
        <property type="entry name" value="Cation_efflux_CTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036837">
        <property type="entry name" value="Cation_efflux_CTD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058533">
        <property type="entry name" value="Cation_efflux_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027469">
        <property type="entry name" value="Cation_efflux_TMD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050291">
        <property type="entry name" value="CDF_Transporter"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01297">
        <property type="entry name" value="CDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43840:SF15">
        <property type="entry name" value="MITOCHONDRIAL METAL TRANSPORTER 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01545">
        <property type="entry name" value="Cation_efflux"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16916">
        <property type="entry name" value="ZT_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160240">
        <property type="entry name" value="Cation efflux protein cytoplasmic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161111">
        <property type="entry name" value="Cation efflux protein transmembrane domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="41"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="110"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein transmembrane" evidence="8">
        <location>
            <begin position="8"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Cation efflux protein cytoplasmic" evidence="9">
        <location>
            <begin position="205"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF16916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="284" mass="32087" checksum="63ECA1BF9C85FCC1" modified="2013-06-26" version="1">MREEEKPLFISIIGNFLLSILKIIIGYYFNSIAIISDGIHSLSDIVTSIISLFGVKIAIKPPDEDHHYGHARFEPLISFIIGLALLFTSYEIFKCSLERIMNVTQLEVNYIMISVIIISIIAKELMARYSIYVGKKLGNQILIADAYHHRSDVYSSLAVLIGLLLEKIGFYYGDGLAGIVVSFMIAKMATNLCLENIDYLTGKSPNNEILEKIKNEALKVEKVLGVHDLKAQYVGKWLYVELHVEVPSNISAKELHDIEVEVKKRLESLEEVEKAYVHVDLVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTW0</accession>
    <name>N6UTW0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Class III signal peptide-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05978</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593048.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTW0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00563"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1164"/>
    <dbReference type="OrthoDB" id="65948at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="137" mass="14922" checksum="A83BB2C1BDFEED3C" modified="2013-06-26" version="1">MNIKAQISLEFSILFLATLLAIIIGVSYPGLYGLNKSTKISSMSLAHAAVMKMKENIEMVGMCEDGSVKVVLIKCPPGIWEANGNEISFTNSKLNYTISTNCSNEVVISGERDIRQFTIITATVEKVGGRVLVNITY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU23</accession>
    <name>N6UU23_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ammonium transporter</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_05658</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the uptake of ammonium/ammonia (NH(4)(+)/NH(3)). Transport is electrogenic.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cell membrane</location>
            <topology evidence="9">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95854.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592704.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU23"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00262"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1104"/>
    <dbReference type="OrthoDB" id="10960at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001905">
        <property type="entry name" value="Ammonium_transpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00836">
        <property type="entry name" value="amt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF6">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="29"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="114"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="175"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="209"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="244"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="278"/>
            <end position="306"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="326"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="361"/>
            <end position="379"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="31"/>
            <end position="410"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU362002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95854.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="418" mass="43302" checksum="782E19309E2EF656" modified="2013-06-26" version="1">MATADLFQNVTDINSIVQALTAMANASDVYFLVVMGVLVFMMQWGFAMLEGGQVRKKNVNNVMMKNMVDWFIGCVSWLFIGGVLCASINPADFISWWGKIFSAAPFLVDNGIELASWFFGLVFAATAATIVSGGVAERIKFSAYVLISLIITALVYPFFVYLGPWGAGIINWHDYAGSLIVHGLGGFLALGAIAAIGPRIGRFKDGKPVPILGHNIPMAVFGALALAIGWYGFNVGSSLALGDISGLVCATTTLAMAGGGIGALIASKKDVLFTANGIVAGLVAICSGTDVVSPVGGLIIGLIAGLQVPIVYKLIENAGLDDVCGVVPVHGTAGVVGAILAGILGMKALGGAGEVSLIDQIIASVFCIVYGTALGYIIAKIAGVICGGLRVSEEDEFIGLDLAEHKMPAYPEEETSVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU49</accession>
    <name>N6UU49_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dual specificity protein phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05404</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000039">
        <property type="protein sequence ID" value="ENN95879.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592470.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU49"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00844"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1055"/>
    <dbReference type="OrthoDB" id="117569at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.190.10:FF:000157">
        <property type="entry name" value="Protein-tyrosine phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.190.10">
        <property type="entry name" value="Protein tyrosine phosphatase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029021">
        <property type="entry name" value="Prot-tyrosine_phosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050561">
        <property type="entry name" value="PTP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000387">
        <property type="entry name" value="Tyr_Pase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23339">
        <property type="entry name" value="TYROSINE SPECIFIC PROTEIN PHOSPHATASE AND DUAL SPECIFICITY PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22785">
        <property type="entry name" value="Tc-R-P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52799">
        <property type="entry name" value="(Phosphotyrosine protein) phosphatases II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50056">
        <property type="entry name" value="TYR_PHOSPHATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tyrosine specific protein phosphatases" evidence="1">
        <location>
            <begin position="74"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95879.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="148" mass="17170" checksum="E893608247C967A2" modified="2013-06-26" version="1">MGKCRHNGEVSIFGVRPASFPYFPFHLKDKIGGFVILDELWLIRWCEIIEYPMRIPTLYVPIEDYSIPTVEDMDLIVDFIKYHVSRGKEVVVSCIGGHGRTGTVLAIWCGLNGVENPIEYVRERYCKCAVETKEQEEFVKYYLSIRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU93</accession>
    <name>N6UU93_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Mechanosensitive ion channel MscS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_05204</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000034">
        <property type="protein sequence ID" value="ENN95919.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592261.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU93"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00308"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1016"/>
    <dbReference type="OrthoDB" id="121853at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008381">
        <property type="term" value="F:mechanosensitive monoatomic ion channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049142">
        <property type="entry name" value="MS_channel_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049278">
        <property type="entry name" value="MS_channel_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045275">
        <property type="entry name" value="MscS_archaea/bacteria_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006686">
        <property type="entry name" value="MscS_channel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30221:SF1">
        <property type="entry name" value="SMALL-CONDUCTANCE MECHANOSENSITIVE CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21088">
        <property type="entry name" value="MS_channel_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21082">
        <property type="entry name" value="MS_channel_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01246">
        <property type="entry name" value="UPF0003"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="47"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="85"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="152"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel transmembrane helices 2/3" evidence="10">
        <location>
            <begin position="128"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="8">
        <location>
            <begin position="170"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS C-terminal" evidence="9">
        <location>
            <begin position="244"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF21088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95919.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="342" mass="39102" checksum="BB07CE9C1B12F260" modified="2013-06-26" version="1">MIEQIFIATVIIIFGVILVKIINYILKNWVKNIVINTKTKLDDILLDAIRLPLAVIILSYSILLALHILGISFKILTILDKTIRAIAIISATYFFVKFTDGIFKYYIIPAVEKTEMEFDDHVIKPLNKIIKIFIITIGILTALSAVGYDITALLASLGIGGLALALAMQDTIKNFIAGVLLLFDKPFSLGHWVKVDGVEGIVEEVGIRSVRIRTFDYTLITIPNSKLLESNIENLTVRDRRRVLMTIGLTYNTSCEKIKKAKEIIIKTLEEHRATLPPYRVHFVEYGDWSLNLRVEYFVRNLGFDYYLNTIEEVNLKIKEEFEKEGIEFAYPTYSIYLEKNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUD8</accession>
    <name>N6UUD8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_04935</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95959.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592090.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUD8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00504"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.967"/>
    <dbReference type="OrthoDB" id="89900at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1530.10">
        <property type="entry name" value="Conserved hypothetical protein from pyrococcus furiosus pfu- 392566-001, ParB domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036086">
        <property type="entry name" value="ParB/Sulfiredoxin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003115">
        <property type="entry name" value="ParB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016427">
        <property type="entry name" value="UCP004699_CBS/ParB"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02195">
        <property type="entry name" value="ParB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004699">
        <property type="entry name" value="UCP004699_CBS_ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00470">
        <property type="entry name" value="ParB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110849">
        <property type="entry name" value="ParB/Sulfiredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="8"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="66"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95959.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="258" mass="29641" checksum="4A480C3560C70417" modified="2013-06-26" version="1">MVKVKEYMTKNVITVKKDDKVKDVIELFKKTKHKSFPVVENGKLVGIVSLVDIVGKDDNERVENVMTKRDDMVITHPDEDIMNVGRIMFRTGLSKLPVVDEKNNLVGLISNMDVIRSQIEKTTPKKLERIINTYKSLGYKLEVKKGKVEIHKLKPTQNKIHSDELIGRIYELKKGLAEPIIVIKKKNDDKYILLDGHHRAVAAYKLGIPELDAYIIYLDTDKKLGIERTAESLNLKTLRDIKIVEHDIENSENIFKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUU1</accession>
    <name>N6UUU1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Small GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_04108</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000027">
        <property type="protein sequence ID" value="ENN96114.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591616.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUU1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01353"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.804"/>
    <dbReference type="OrthoDB" id="147673at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01897">
        <property type="entry name" value="NOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041623">
        <property type="entry name" value="NOG1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010674">
        <property type="entry name" value="NOG1_Rossman_fold_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45759">
        <property type="entry name" value="NUCLEOLAR GTP-BINDING PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06858">
        <property type="entry name" value="NOG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17835">
        <property type="entry name" value="NOG1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="168"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96114.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="337" mass="38551" checksum="A51F43F2E4DB0142" modified="2013-06-26" version="1">MENPFKKIPTILMPDELMEKALRRGEKVANEMRQKPLPSLIKSKMVEEHKVRTIGSVVADNLQKVIDKAPAVRKLPKFYQEMVEILVGIDEFKKSLGAFKWASDLVKKLTNEYAKKIRRSRTPQQAAKFRKEFVGRVKSILKQIHPEMAFIAVAREKLKDLPTFKDLPTIAIAGYPNVGKSTILKALTGADVEINNYPFTTKGINVGYMDNIQMLDTPGLLDRPLYERNDIELQAILALNYLADIILFVIDASEYCGYSLDEQISLLKEISELFKLPIILAINKIDLIDKERLNKIRDMLKNYEVIEISADKGINLDKLKEILKKRAIESYKSKLRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUV9</accession>
    <name>N6UUV9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRC-barrel domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96139.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591328.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUV9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00379"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.729"/>
    <dbReference type="OrthoDB" id="85079at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.240">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38137">
        <property type="entry name" value="PRC-BARREL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38137:SF2">
        <property type="entry name" value="PRC-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC-barrel" evidence="1">
        <location>
            <begin position="9"/>
            <end position="77"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96139.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="88" mass="9639" checksum="BB1309AD2F46FD8C" modified="2013-06-26" version="1">MKKLPAKIIFGKNIVGNLGSVLGRVVDIVFDEKIGKIVSLEVEPEEGSPIDIADGKNVFIPYKAVIAIKDVVVIDEKYLTQAYIKPVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUZ8</accession>
    <name>N6UUZ8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03533</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96189.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591231.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUZ8"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.689"/>
    <dbReference type="OrthoDB" id="64846at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96189.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="297" mass="34719" checksum="5551FDF038DFBC47" modified="2013-06-26" version="1">MEKVLSKIGTIKDLEEIIDSLSNFTGIIRIGNGYLFYINSELIASKLGNKDKDLRDILISLPEKFIIELYIGDSHEVKKAIKCFRDDKIFIETTKLCIVHKDYLELNNYNEIFKYLTYFNKVTFYPKKFKDAEAIVIYKNKEEIFSIFKNKKIYYGEKAIKKLKSLFAISKVVAKVEKTSLDEFENLRNTYPEGEIIRSFNKILETIKSNKKYIEACGTLSDALLHGTCLIEIGSEGYIIAKEGKPVYAFYKDYDGDKSYRIIKSMCIMEDIKYKIYKLSNSEYNLFKQFKNNRIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV23</accession>
    <name>N6UV23_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pyridoxal 5'-phosphate synthase subunit PdxT</fullName>
            <ecNumber evidence="7">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pdx2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Pyridoxal 5'-phosphate synthase glutaminase subunit</fullName>
            <ecNumber evidence="7">3.5.1.2</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pdxT</name>
        <name evidence="10" type="ORF">J422_03688</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the glutaminase PdxT/SNO family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="7"/>
    <dbReference type="EC" id="3.5.1.2" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96214.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591278.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV23"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01368"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.720"/>
    <dbReference type="OrthoDB" id="26717at2157"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1903600">
        <property type="term" value="C:glutaminase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006543">
        <property type="term" value="P:L-glutamine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008614">
        <property type="term" value="P:pyridoxine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01749">
        <property type="entry name" value="GATase1_PB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000010">
        <property type="entry name" value="uncharacterized protein LOC100176842 isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01615">
        <property type="entry name" value="PdxT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002161">
        <property type="entry name" value="PdxT/SNO"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021196">
        <property type="entry name" value="PdxT/SNO_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03800">
        <property type="entry name" value="PLP_synth_Pdx2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31559:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNO1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01174">
        <property type="entry name" value="SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005639">
        <property type="entry name" value="Glut_amidoT_SNO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01236">
        <property type="entry name" value="PDXT_SNO_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51130">
        <property type="entry name" value="PDXT_SNO_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2 7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Nucleophile" evidence="7 8">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7 8">
        <location>
            <position position="167"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="46"/>
            <end position="48"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 9">
        <location>
            <begin position="128"/>
            <end position="129"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR005639-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96214.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="185" mass="20839" checksum="F0C3F9BC6B8C099D" modified="2013-06-26" version="1">MIFGVLAIQGAIEEHEEALKKAGYEAKKVRNKEDLKDIDALIIPGGESTTIGKLMKKYGLFESIKNSSLPILGTCAGMVLLSKDSGYKQDLLGLMDITVKRNAYGRQRESFEKEIYFKDIGKIYGVFIRAPVVDKILSDDVEVIAKDDDKIVGVMQDRYMALSFHPELSEDGYKVYKYFVKHCVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV58</accession>
    <name>N6UV58_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">protein-glutamate O-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.80</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_03263</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-glutamyl-[protein] + S-adenosyl-L-methionine = [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:24452"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="2.1.1.80"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.80" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96249.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV58"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01040"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.639"/>
    <dbReference type="OrthoDB" id="10657at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008983">
        <property type="term" value="F:protein-glutamate O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.155.10">
        <property type="entry name" value="Chemotaxis receptor methyltransferase CheR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050903">
        <property type="entry name" value="Bact_Chemotaxis_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026024">
        <property type="entry name" value="Chemotaxis_MeTrfase_CheR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022642">
        <property type="entry name" value="CheR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000780">
        <property type="entry name" value="CheR_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022641">
        <property type="entry name" value="CheR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036804">
        <property type="entry name" value="CheR_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24422">
        <property type="entry name" value="CHEMOTAXIS PROTEIN METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24422:SF10">
        <property type="entry name" value="CHEMOTAXIS PROTEIN METHYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01739">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03705">
        <property type="entry name" value="CheR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000410">
        <property type="entry name" value="CheR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00996">
        <property type="entry name" value="CHERMTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00138">
        <property type="entry name" value="MeTrc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47757">
        <property type="entry name" value="Chemotaxis receptor methyltransferase CheR, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50123">
        <property type="entry name" value="CHER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CheR-type methyltransferase" evidence="6">
        <location>
            <begin position="6"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96249.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="282" mass="33715" checksum="FC7D2A6DF58D0CC7" modified="2013-06-26" version="1">MISRDDVESLDPIEKMYFKRILSFIKNTLKIDVTQYKESYIIRRIRVRMKATKCQSFKEYYEYLKTHKEEHEELANTLTVNVTEFWRDITVYKEIMKIIDNWVADRSIRKLRIWSAGCSSGEEPYGVAIILNEAIEKHKRKLLLASIIGTDIDKEVLNKARIGIYHIKQLKNLPPHIIEKYFIKLNEEEYQIKQIIKKYVKFQLHDLIKDPPLKNMDMILCRNVIIYFDKKIQEEIFLKFYEALNPGGFLVLGKTEILHGEAKKLFKTYNARERIYQKPKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVE8</accession>
    <name>N6UVE8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Polyprenyl synthetase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_03179</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FPP/GGPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96329.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590799.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVE8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00182"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.624"/>
    <dbReference type="OrthoDB" id="26738at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00685">
        <property type="entry name" value="Trans_IPPS_HT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.600.10">
        <property type="entry name" value="Farnesyl Diphosphate Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008949">
        <property type="entry name" value="Isoprenoid_synthase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000092">
        <property type="entry name" value="Polyprenyl_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033749">
        <property type="entry name" value="Polyprenyl_synt_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001">
        <property type="entry name" value="GERANYLGERANYL PYROPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12001:SF85">
        <property type="entry name" value="SHORT CHAIN ISOPRENYL DIPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00348">
        <property type="entry name" value="polyprenyl_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00005">
        <property type="entry name" value="Isoprenoid_Synthase_Type_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01017">
        <property type="entry name" value="Polyprenyl_Transferase_Like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48576">
        <property type="entry name" value="Terpenoid synthases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00723">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00444">
        <property type="entry name" value="POLYPRENYL_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96329.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="316" mass="35760" checksum="B223EBA7533B5915" modified="2013-06-26" version="1">MDREILSLIDEELKKYVNKNNKLYSAANHLLLSGGKRLRPYLCVLVYKLKKDNLSEVLPAAVSVELIHNYTLIHDDIMDNDDLRRGKPTVHKVYGNAMAILAGDLLYAKAFEAVANIKDNRKAHEVLKILADACVYVCEGQAMDMEFEEKDSVSLEEYLDMIRKKTGALIEAAAKIGAVMGDFNDEEMKAIEKYAENIGLTFQIQDDVLDLIADEEKLGKPVGSDIREGKKTIIIIHALSTLEEDKKNRLKSLLGKKDITKEEIGEAIEILRPSIEYAKNLMENKTIEAIDALKIFDKDRRKPLEDLAKFIISRCY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVH0</accession>
    <name>N6UVH0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Bacteriohemerythrin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_02674</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Oxygen-binding protein. May be involved in a storage mechanism or for delivery to oxygen-requiring enzymes. The oxygen-binding site contains two iron atoms.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the hemerythrin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96349.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590577.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVH0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00087"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.524"/>
    <dbReference type="OrthoDB" id="15541at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005344">
        <property type="term" value="F:oxygen carrier activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12107">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.50">
        <property type="entry name" value="Hemerythrin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00556">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023504">
        <property type="entry name" value="Bacteriohemerythrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016131">
        <property type="entry name" value="Haemerythrin_Fe_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050669">
        <property type="entry name" value="Hemerythrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012312">
        <property type="entry name" value="Hemerythrin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035938">
        <property type="entry name" value="Hemerythrin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012827">
        <property type="entry name" value="Hemerythrin_metal-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033749">
        <property type="entry name" value="bact_hemeryth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02481">
        <property type="entry name" value="hemeryth_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37164">
        <property type="entry name" value="BACTERIOHEMERYTHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37164:SF1">
        <property type="entry name" value="BACTERIOHEMERYTHRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01814">
        <property type="entry name" value="Hemerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47188">
        <property type="entry name" value="Hemerythrin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00550">
        <property type="entry name" value="HEMERYTHRINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0561">Oxygen transport</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Hemerythrin-like" evidence="7">
        <location>
            <begin position="12"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00556"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96349.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="144" mass="17406" checksum="8177668870E85134" modified="2013-06-26" version="1">MEIIKWSKDLETGIKTFDEEHKYLVKTLNDIFELIKKGEKEKAKLLLKERVIEYATKHFKHEEKIMERYKYPEYEKHKKTHELFVKTLIEKLIPKIENGSDNDFRSAVSFLIGWISMHIINVDKKYGKWFKENSIEINDEPFQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6UW17</accession>
    <name>N6UW17_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Large ribosomal subunit protein eL24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpl24e</name>
        <name evidence="12" type="ORF">J422_01860</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9 10">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L14.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the eukaryotic ribosomal protein eL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96519.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590126.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UW17"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00220"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.366"/>
    <dbReference type="OrthoDB" id="55506at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00472">
        <property type="entry name" value="Ribosomal_L24e_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.170.20:FF:000001">
        <property type="entry name" value="probable ribosome biogenesis protein RLP24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.170.20">
        <property type="entry name" value="Ribosomal protein L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00773">
        <property type="entry name" value="Ribosomal_eL24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038630">
        <property type="entry name" value="L24e/L24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056366">
        <property type="entry name" value="Ribosomal_eL24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055345">
        <property type="entry name" value="Ribosomal_eL24-rel_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000988">
        <property type="entry name" value="Ribosomal_eL24-rel_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023442">
        <property type="entry name" value="Ribosomal_eL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011017">
        <property type="entry name" value="TRASH_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF034186">
        <property type="entry name" value="PRK14891.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10792:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01246">
        <property type="entry name" value="Ribosomal_L24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00746">
        <property type="entry name" value="TRASH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57716">
        <property type="entry name" value="Glucocorticoid receptor-like (DNA-binding domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01073">
        <property type="entry name" value="RIBOSOMAL_L24E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 10 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 10" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 10" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TRASH" evidence="11">
        <location>
            <begin position="7"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="10">
        <location>
            <begin position="7"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00062681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96519.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="67" mass="7953" checksum="D190CB1C760AA051" modified="2013-06-26" version="1">MPEWRRCSFCGHEIEPGTGKMVVEKDGTVLYFCSSKCEKSYMMGRNPRKLKWTKIYQDMKKELKAKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6UW42</accession>
    <name>N6UW42_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Aminotransferase class V</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_01980</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96539.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UW42"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00241"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.388"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008453">
        <property type="term" value="F:alanine-glyoxylate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004760">
        <property type="term" value="F:L-serine-pyruvate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019265">
        <property type="term" value="P:glycine biosynthetic process, by transamination of glyoxylate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000027">
        <property type="entry name" value="Serine--pyruvate aminotransferase, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000192">
        <property type="entry name" value="Aminotrans_V_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010916">
        <property type="entry name" value="TonB_box_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152:SF24">
        <property type="entry name" value="ALANINE--GLYOXYLATE AMINOTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21152">
        <property type="entry name" value="AMINOTRANSFERASE CLASS V"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00266">
        <property type="entry name" value="Aminotran_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00430">
        <property type="entry name" value="TONB_DEPENDENT_REC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class V" evidence="6">
        <location>
            <begin position="26"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96539.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="312" mass="34792" checksum="583EB40B5A8393E1" modified="2013-06-26" version="1">MEKLIMLPGPTKIPSEVLLAMAKPIIGHRTKDFGELLEDTIEKLKKVFITDNDTFIVTGSGTAAMDMAISNVINKGDKVLTIITGVFGERFAKIVETYGGEVVKLEVEYGDMADAKVVKEILDENEDIKAVTVVHNETSTGARNDIEAIGKVVKDYDAIYIVDTISSLGGDYVNVDKFNIDICVTGSQKCLAAPPGLAAITVSEKAWEVIEKNTPKNFYLNLKEYKNYYYEKKQTPYTPAVNLVFALNKALELLLEEGLENRVRRHENLAKIVRKGLENLNIELFAKERARSITVTSAKYPEGIDDKEFRVY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWA0</accession>
    <name>N6UWA0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein eL43</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rpl37ae</name>
        <name evidence="6" type="ORF">J422_01590</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="5">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the eukaryotic ribosomal protein eL43 family. Putative zinc-binding subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589977.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWA0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00445"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.313"/>
    <dbReference type="OrthoDB" id="372011at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00327">
        <property type="entry name" value="Ribosomal_eL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002674">
        <property type="entry name" value="Ribosomal_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050522">
        <property type="entry name" value="Ribosomal_protein_eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00280">
        <property type="entry name" value="eL43_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003058">
        <property type="entry name" value="PRK03976.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48129:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL43"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01780">
        <property type="entry name" value="Ribosomal_L37ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <keyword evidence="1 5" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="5">
        <location>
            <begin position="39"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="91" mass="10086" checksum="EFD7BC650B02DE4F" modified="2013-06-26" version="1">MFSHTKKVGPAGRFGPRYGLKIRARVRDVEVKAKQKYKCPVCGFQKLRRASTSIWVCEKCGAKVAGGAYTPETGAGKAVMKAIRRILEKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWD3</accession>
    <name>N6UWD3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Water Stress and Hypersensitive response domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01218</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589909.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWD3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00045"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.238"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1820">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004864">
        <property type="entry name" value="LEA_2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03168">
        <property type="entry name" value="LEA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117070">
        <property type="entry name" value="LEA14-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Late embryogenesis abundant protein LEA-2 subgroup" evidence="1">
        <location>
            <begin position="45"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="161" mass="18141" checksum="20D9E6EDEAA9E91A" modified="2013-06-26" version="1">MRRLLILLIVLPFLMTSGCLEKPKIVSNDIEFKKVEADTTLLNVKITVENPNPIGVHINKIFFNIFYINNDGKLEYLGYGEKEDININSGNTTIEIPVHVLNKNLAKALIESGGVLRVKIDGKINVDLFLTSIDVPISYETTLNIPEYVKEELKEMIANLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWE8</accession>
    <name>N6UWE8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">MATE efflux family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_01056</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589879.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWE8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00832"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.205"/>
    <dbReference type="OrthoDB" id="214119at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042910">
        <property type="term" value="F:xenobiotic transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002528">
        <property type="entry name" value="MATE_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048279">
        <property type="entry name" value="MdtK-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052031">
        <property type="entry name" value="Membrane_Transporter-Flippase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00797">
        <property type="entry name" value="matE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43549:SF2">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN NORM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43549">
        <property type="entry name" value="MULTIDRUG RESISTANCE PROTEIN YPNP-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01554">
        <property type="entry name" value="MatE"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006603">
        <property type="entry name" value="DinF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="42"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="159"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="227"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="273"/>
            <end position="293"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="314"/>
            <end position="334"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="354"/>
            <end position="374"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="381"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="407"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="10">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="437" mass="48089" checksum="8818F43E8595BFA3" modified="2013-06-26" version="1">MLLDDPKKAVINVSKPIILATFVESLYSIVDSIWVSGLGDKALAAIGASFPILISIYAISWGFGIAVSSAISRRIGEGNLKLANKTAFHGLLLAVILGILYTLLIYPNLSIIFDLMHVSGDTKILAINYLKPLTLSAVLFNICDVLYGIFRGEGRTKVVMIASVIATVSNIILDPILIYIFKLNIIGASLATIMAIFIAIFILTANLRKSRIKIILSKINLEIIKDLLRVGLPSSLIDFTVAIEFFIITSIIMSITSAEDLAIFTGALRITDFGFIPMLGLASGACSVIGANYGAKRFENVKIAYLYTIKIGTVMEFLIILFIILLSPYLAYLFTYTKASYIIYNKLVLSLKILPWYLLFTPMILTTGALFQALGRGELSLLVSIFRSSSHIIFIYILSAILCLKIFGVFLGFIFAEMFSGFCALMFGYYVTKRLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWI5</accession>
    <name>N6UWI5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF655 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00921</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589854.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWI5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00590"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.181"/>
    <dbReference type="OrthoDB" id="7902at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.280">
        <property type="entry name" value="AF1531-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007003">
        <property type="entry name" value="DUF655"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734:SF1">
        <property type="entry name" value="DNA-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40734">
        <property type="entry name" value="TRNA-SPECIFIC ADENOSINE DEAMINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04919">
        <property type="entry name" value="DUF655"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160975">
        <property type="entry name" value="AF1531-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="24101" checksum="8E2DC6C7D9F414C5" modified="2013-06-26" version="1">MVQNYPKKDKKKFENYAWILDYLPYGYGNIKEPIAQAVGEYQFVLMELKPKTDLELGERVYIGKGKRDKIDHVRRMIKYDHLTPTAKSELLYVIMDAVRVQEERFIKFFNEAPPITTKLHSLELLPEISKKYMWRIIEEREAKKFESFEDFKNRVKKDPVKIIAKRIVKELSEENKYYLFVKWKKGIILDEDTMTFYLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWL4</accession>
    <name>N6UWL4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Nickel-dependent hydrogenase large subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_00761</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWL4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00884"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.148"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053511">
        <property type="entry name" value="NiFe/NiFeSe_hydrogenase_LSU"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041785">
        <property type="entry name" value="VhuA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43600">
        <property type="entry name" value="COENZYME F420 HYDROGENASE, SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43600:SF2">
        <property type="entry name" value="F420-NON-REDUCING HYDROGENASE VHU SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 6" id="KW-0533">Nickel</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="392"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR601501-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="416" mass="46888" checksum="59658D380921AEA2" modified="2013-06-26" version="1">MKVTIEPLSRLEGHGKVTLTIEDGKAIDVKLHITALRGFEKFVVGRPAEEVPRIVTRICGICQTAHHLASVKAVDMAWDAKIPETAKKLRELMLLGNMIHSHALHFYFLASPDFILGVDADPKIRNIIGVINKDPELAKKAIALRRFGQRVVEIVGGKAIHPITAIPGGISKQLSEEERDELLKEIDLMIEYSKESIEVVKKLTEQYMNYVKTLGVIDTWYLGIVNDGKHEFYDGKLRFLSPDGKEKDEFEAKDYKDYIGEHVVSYNYVKHPFYKKIGYPDGIYRVGPLSMLNVCDHMGTELAEEYRKEFFDIFGFPANQSLAYHYARMVEMLKACERVKELLNDNNITSDDIKADVEPKAGNGVGVVEAPRGVLIHNYETDDNGIVTKANMIVATTHNVPTMEKAIQQAAKEIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWS9</accession>
    <name>N6UWS9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Xylose isomerase-like TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00060</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589705.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWS9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01155"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.12"/>
    <dbReference type="OrthoDB" id="33250at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003906">
        <property type="term" value="F:DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008081">
        <property type="term" value="F:phosphoric diester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.150:FF:000017">
        <property type="entry name" value="Endonuclease IV related protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445:SF0">
        <property type="entry name" value="APURINIC-APYRIMIDINIC ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21445">
        <property type="entry name" value="ENDONUCLEASE IV ENDODEOXYRIBONUCLEASE IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="1">
        <location>
            <begin position="22"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="263" mass="30528" checksum="0D17E502383055EF" modified="2013-06-26" version="1">MHFGTAGVPLRAKNEFDGAKVLKSFNLDAMELEFVNGIYMNENYAKFLKDLEIIFSAHAPHYINLNAIEKEKVERSIYYIIKSSKVLRHCGRNLVFHPGYYLKKSKDETYKNIRDNIKLILEKIDKNISLRPETAGKIYQFGDIDEILNLCHELNILPCIDFSHIYARSRGKVNSYDDFYKILEKVEDMLGKEAIKDMHIHLTGIEFNKSGEVKHLPLEESDFNYKDVLKALKDFEAEGTVICESPLLEDDAKLLKDYYLTLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWW9</accession>
    <name>N6UWW9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9 10">DNA repair and recombination protein RadB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">radB</name>
        <name evidence="12" type="ORF">J422_00290</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Involved in DNA repair and in homologous recombination. May regulate the cleavage reactions of the branch-structured DNA. Has a very weak ATPase activity that is not stimulated by DNA. Binds DNA but does not promote DNA strands exchange.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the eukaryotic RecA-like protein family. RadB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589745.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWW9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01105"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.58"/>
    <dbReference type="OrthoDB" id="17644at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00350">
        <property type="entry name" value="RadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011939">
        <property type="entry name" value="DNA_repair_and_recomb_RadB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02237">
        <property type="entry name" value="recomb_radB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF47">
        <property type="entry name" value="DNA REPAIR AND RECOMBINATION PROTEIN RADB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003336">
        <property type="entry name" value="RadB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6 9" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="11">
        <location>
            <begin position="1"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00024641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR02237"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="212" mass="24248" checksum="2AF573E2A1B401BB" modified="2013-06-26" version="1">MIRNIILGYANKGLLTQIYGQPGAGKTNICLLNTINYSKEGKVVYIDTESGLSIERLKQITENYKDVLKNMIIYNVFDFFEQDKVIQKELIEICKNNISLVVVDNISSLYRLELTDKAEKNIVLNRMLGNQLKVLAKIAKIYNIPVIITNQIRETINGFEASGGHLLEYWSKCIIRLEKLGEDRLAILEKHLFAGEDRVRFRIVDNGIVILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6UZZ0</accession>
    <name>N6UZZ0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Selenocysteine-specific elongation factor</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SelB translation factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_06982</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Translation factor necessary for the incorporation of selenocysteine into proteins. It probably replaces EF-Tu for the insertion of selenocysteine directed by the UGA codon. SelB binds GTP and GDP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000066">
        <property type="protein sequence ID" value="ENN95598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594666.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UZZ0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01229"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1352"/>
    <dbReference type="OrthoDB" id="30874at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04094">
        <property type="entry name" value="eSelB_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03696">
        <property type="entry name" value="SelB_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.190">
        <property type="entry name" value="translation elongation factor selb, chain A, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038661">
        <property type="entry name" value="Ribosomal_eL33_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048956">
        <property type="entry name" value="SelB_III_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004535">
        <property type="entry name" value="Transl_elong_SelB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00475">
        <property type="entry name" value="selB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF11">
        <property type="entry name" value="SELENOCYSTEINE-SPECIFIC ELONGATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21440">
        <property type="entry name" value="aSelB_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="9">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00015953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025526"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="462" mass="51174" checksum="08FAFCF227F2E948" modified="2013-06-26" version="1">MININIGLFGHIDHGKTELAKKLTEIPSTSALDKPKESKIRGITVDLGFSSFTLDDYNITLVDAPGHAELIRTAIGASNIIDLAILVVDAKESAKPQTGEHLIVLDLLNIPTIVAINKIDIASEEEIKKTELIMRNILNSTKNLKNSKIVKISAKTGEGIDKLKEEIKNLLKKIKIERDTNSYLKMPIDHAFKIKGVGTVVTGTIHKGTLKVGDNVIILPINYDVKVKSIQCFKKDVEMAKAGDRVGVALLNVEPESIYRGCVLTSKDTKLKVAEKFIAKIKVLELFKYNLKPKMKVHIHAGLLTVPAKIIPFNYEIVNDKKEAVVLDNVKAGDKCYCLFILEEPIVLEEGDKVLIMRLDLPATVLRIAGFGEVMGFNDIELKRLIVKEGRIIKKKDKFFVEGLAHSKQIAEKLVGELVYLPEKDKYVKITGTFGTKGLLTIDAEGVEGGEKVILKKVRKWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0D3</accession>
    <name>N6V0D3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05973</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593046.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0D3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00564"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1163"/>
    <dbReference type="OrthoDB" id="66027at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="285" mass="33265" checksum="371D89C9A9AB6ADA" modified="2013-06-26" version="1">MRRVLLFLIFFLPYIYAENVVIGEINLKYTISGEMTNNNPYPIFIALPDEYVVINTYKTLPLPNSGYCNLDIYQNSTNATYYLIKPELKYMTIFNGKKGFWLPPYTTVKLKMILPNISYALSINIPDEHNFRSVGPAICNKYGVIKLDNIYPNAKRDNIILDNFKLTVYGCLKLNDYSNAYSLIIPAPITFDEYKDIVIIPINDVDDKIWINYYDWLNGFMKVKRKKKLLDDYSDNGLLIDDLDINLDKNYPALAFTTTSTNQNLEFYYIVYWKRKSINLPDILD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0I0</accession>
    <name>N6V0I0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Peptidase A24B, FlaK domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_05813</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592830.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0I0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01243"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1133"/>
    <dbReference type="OrthoDB" id="19094at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054964">
        <property type="entry name" value="Arch_preflagellin_pept"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052218">
        <property type="entry name" value="Preflagellin_Peptidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009655">
        <property type="entry name" value="Preflagellin_peptidase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000045">
        <property type="entry name" value="Prepilin_IV_endopep_pep"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040695">
        <property type="entry name" value="FlaK_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36506">
        <property type="entry name" value="PREFLAGELLIN PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36506:SF1">
        <property type="entry name" value="PREFLAGELLIN PEPTIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06847">
        <property type="entry name" value="Arc_PepC_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01478">
        <property type="entry name" value="Peptidase_A24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="domain" description="Prepilin type IV endopeptidase peptidase" evidence="7">
        <location>
            <begin position="6"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="domain" description="Preflagellin peptidase C-terminal" evidence="8">
        <location>
            <begin position="145"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF06847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="229" mass="26180" checksum="D574DE0D7BFB9FEE" modified="2013-06-26" version="1">MINYAFGLLCILIALYCDIKSREINEYIWITMIIFGLMYHIYLTFVTGNLIYITQSILGIVICFFLGYFMFLLGVGGGDGKLLIGLGALVPKYNIPISSPLGYLLSHPYLPTFPIFVLINAIFLIILIPIIVFLKNISKYKPKNIKEFFMMFIAEKHKFSDIKDKNFVVLGKDGNINIIKNVEKKEEYGDDDIVWASPEIPFVVPIFFSYILTPFIGDLIFKIIFNILA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0K9</accession>
    <name>N6V0K9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05653</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95853.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592702.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0K9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00263"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1103"/>
    <dbReference type="OrthoDB" id="28968at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019198">
        <property type="entry name" value="Beta_propeller_containing"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09826">
        <property type="entry name" value="Beta_propel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69322">
        <property type="entry name" value="Tricorn protease domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95853.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="558" mass="65532" checksum="88DA9CD546D4917A" modified="2013-06-26" version="1">MKKLILIILILLLSGCISKEEDISDFKIFEVNDPKKLFINKYEYNIYKNEYKFSETNVQVRGVDEADIVKTNGCFIFYSPTSYFNKKTYIINSKALNVTNVIKDGNLLYLKNNTLIIINRFKNTITSYDISNPKKIRVIWKKVLNGSYVDSRVYKDKIYLIIAKGDYPQIWYDVKLKYYIPNVPICRNYIISEINIKNGKINRAIALAGGYNTIIYMSRTNIYLTYYISHRDRAYLDFIKENAEKYFPEELSKTLKKIIDDNLFSDEAKLLEVKRRINSYIYQLPKEDALNLKNILDKDFTNYLKKNIYKYERTGIVKIDLKSFSVKSSSVPGRLINRYAMDEYNGNLRLVTTLGDPIVGYRNNVYVLNSNLKLIGKLENIEEGKKIFAVRFLKDKVFIITYYRKDPLLVISLDDHPKILGYLNISGYSTYLHFINNDKLLAIGKDDDGKIKIDLYNISNYNNPKLLDRFKLDKFWSLALYNPHAFLWNKENKILAIPIERSAYIFKIDNEIKLKKIIEHLDYVERVIYINNHIFSLSPSEVVKTNITSWKSEKLVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0N0</accession>
    <name>N6V0N0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5'-nucleotidase SurE</fullName>
            <ecNumber evidence="4">3.1.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Nucleoside 5'-monophosphate phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">surE</name>
        <name evidence="6" type="ORF">J422_05494</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Nucleotidase that shows phosphatase activity on nucleoside 5'-monophosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate</text>
            <dbReference type="Rhea" id="RHEA:12484"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:18254"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58043"/>
            <dbReference type="EC" id="3.1.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="4">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the SurE nucleotidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.5" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000041">
        <property type="protein sequence ID" value="ENN95873.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592561.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0N0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01407"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1073"/>
    <dbReference type="OrthoDB" id="26873at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008253">
        <property type="term" value="F:5'-nucleotidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1210.10">
        <property type="entry name" value="Survival protein SurE-like phosphatase/nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00060">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030048">
        <property type="entry name" value="SurE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002828">
        <property type="entry name" value="SurE-like_Pase/nucleotidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036523">
        <property type="entry name" value="SurE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001491">
        <property type="entry name" value="PRK00346.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00087">
        <property type="entry name" value="surE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457">
        <property type="entry name" value="5'-NUCLEOTIDASE SURE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30457:SF0">
        <property type="entry name" value="PHOSPHATASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_4G01070)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01975">
        <property type="entry name" value="SurE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64167">
        <property type="entry name" value="SurE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Survival protein SurE-like phosphatase/nucleotidase" evidence="5">
        <location>
            <begin position="3"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95873.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="266" mass="29991" checksum="10FA0E091AEC7E76" modified="2013-06-26" version="1">MDILIVNDDGIYSPSLVALYKALKNKFDNANITIVAPTNQQSGIGRAISLFEPLRMTKVRLEDDIYGYAVSGTPTDCVILGIYQILKKIPDLVISGINIGENLGTEIMTSGTLGAAFEAAHHGAKSMAVSLQITSDHLKFREFDIPLDFKIPAEITVRIAEKYLKYDFPCDLLNINIPEKANLNTPIEITRLARKMYTTHVEERIDPRGRSYYWIDGYPIFEEDEDTDVYVLRKKGHISITPLTLDTTIKNLDEFKKKYGEILTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0U2</accession>
    <name>N6V0U2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit Rpo1N</fullName>
            <ecNumber evidence="13">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">DNA-directed RNA polymerase subunit A'</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">rpo1N</name>
        <name evidence="13" type="synonym">rpoA1</name>
        <name evidence="17" type="ORF">J422_05159</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12 13">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms the clamp head domain.</text>
    </comment>
    <comment type="function">
        <text evidence="14">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 19">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds at least 2 Zn(2+) per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 13 14">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="13"/>
    <dbReference type="EMBL" id="APMM01000033">
        <property type="protein sequence ID" value="ENN95928.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592241.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0U2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01225"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1009"/>
    <dbReference type="OrthoDB" id="371812at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02582">
        <property type="entry name" value="RNAP_archeal_A"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB1"/>
        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="DNA-directed RNA polymerase subunit"/>
        <property type="match status" value="1"/>
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        <property type="match status" value="2"/>
    </dbReference>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2940">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.180">
        <property type="entry name" value="RNA polymerase ii"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.120">
        <property type="entry name" value="RNA polymerase II, clamp domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00863">
        <property type="entry name" value="RNApol_arch_Rpo1N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
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    <dbReference type="InterPro" id="IPR000722">
        <property type="entry name" value="RNA_pol_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006592">
        <property type="entry name" value="RNA_pol_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007080">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007066">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007083">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
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    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044893">
        <property type="entry name" value="RNA_pol_Rpb1_clamp_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038120">
        <property type="entry name" value="Rpb1_funnel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012758">
        <property type="entry name" value="RPO1N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006336">
        <property type="entry name" value="PRK08566.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02390">
        <property type="entry name" value="RNA_pol_rpoA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF32">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04997">
        <property type="entry name" value="RNA_pol_Rpb1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00623">
        <property type="entry name" value="RNA_pol_Rpb1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04983">
        <property type="entry name" value="RNA_pol_Rpb1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05000">
        <property type="entry name" value="RNA_pol_Rpb1_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00663">
        <property type="entry name" value="RPOLA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2 13 14" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13 14" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13 14" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 13 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase N-terminal" evidence="16">
        <location>
            <begin position="208"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="659"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="466"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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    <feature type="binding site" evidence="13">
        <location>
            <position position="468"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
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    <feature type="binding site" evidence="13">
        <location>
            <position position="470"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00053389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00863"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN95928.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="889" mass="101151" checksum="8B51C3BAEDE5EBDA" modified="2013-06-26" version="1">MEKYEIPKELKEIMFGVLSPDYIRKMSAARIVTPDTYDEDGYPIEGGLMDPRLGVIDPGLVCKTCGGRIGECPGHFGHIELAKPVIHIGFAKTIYKILKSVCPHCGRVAITENKRREILEKMRKLEEEGGNKWEICEEVYKEASKANVCPYCGEIKYDVKYEKPTSYYRIDGNEEKPLTPADVREILEKIPDEDCILLGINPETTRPEWMVLTVLPVPPVTMRPSIILETGERSEDDLTHKLVDIVRINNRLEENIEGGAPNLIIEDLWNLLQYHINTYFDNEAPGIPPARHRSGRPLKTLAQRLKGKEGRFRYNLAGKRVNFSARTVISPDPCLSINEVGVPIEIAKELTVPEKVTRYNIEKIRKLIKNGPNKHPGVNYIIRKMIGRDGKEYEYKIKLTDNNKDFWVENIKEGDIVERHLMDGDIVLYNRQPSLHRMSIMGHRVRVLPYRTFRHNLCVCPPYNADFDGDEMNLHVPQTEEAKAEAEALMLVEKHILSPRYGGPIIGAIHDYISGAYILTSRYFTKDEATLILRSGGIKDELWEPDKIENGVPLYSGKKIFSKALPKGLNLRFKARICRKCKECKKEDCEYDAYVVIKDGELIKGVIDKNAYGAEAGILLHTIVKEYGHEAGRKFLDSVTKMVIRTLMLKGFTTGIDDIDIPKEALEEIEEVLNEAEKKVEEIIEKYKKGELELLPGKDLEESRETHINNVLRDARDKAGEIAERYLGMDNHAVIMARTGARGNILNITQMAACVGQQSVRGQRIFRGYRGRVLPHFEKNDLSARAHGFVRSSYKKGLSPTEFFFHAMGGREGLVDQAVRTAQSGYMQRRLINALQDLKSEIDKSVRDSRGIIIQFKYGEDGVDPMLADRGKAVNIDRIIDKIKMKYQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0U8</accession>
    <name>N6V0U8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Queuosine synthesis-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05184</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000033">
        <property type="protein sequence ID" value="ENN95933.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592246.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0U8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01220"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1014"/>
    <dbReference type="OrthoDB" id="61764at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012122">
        <property type="entry name" value="ATPase_PP-loop_MJ1016"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052188">
        <property type="entry name" value="Ni-pincer_cofactor_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169:SF4">
        <property type="entry name" value="ATPASE, PP-LOOP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169">
        <property type="entry name" value="EXSB FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036670">
        <property type="entry name" value="ATPase_UCP036670"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95933.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="292" mass="34311" checksum="A0141F1362D32170" modified="2013-06-26" version="1">MIFSRWTQEKRKLNSLDELKKDILEQFKENNLLDEEIIAMVSGGKDSSLSVALAKDLNLNISYILHFYHRWSWDVSRKMVEKIGKIYNIPVVYIDITNDLLKRTKRAKGSSICRICKNIMKCKTVDFAKEHGIKIIMTGDSSLDKIVGAVMNYLREKYGEVKFNKMELTKVPGRYGLFFFRPLIRLAYKDILRLLNYYNLNIERAYEVGDKKGFWREGCCLQYVDGNIILENNEEIFNKLYKYNKLATKIAKEHGFRASITYPSKRILVEPNKEEYRDIIKKALRDLDDESL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6V188</accession>
    <name>N6V188_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Putative thymidylate synthase</fullName>
            <shortName evidence="5">TS</shortName>
            <shortName evidence="5">TSase</shortName>
            <ecNumber evidence="5">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">thyA</name>
        <name evidence="7" type="ORF">J422_04198</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May catalyze the biosynthesis of dTMP using an unknown cosubstrate.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Pyrimidine metabolism; dTTP biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the thymidylate synthase family. Archaeal-type ThyA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96043.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591714.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V188"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00682"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.821"/>
    <dbReference type="OrthoDB" id="50118at2157"/>
    <dbReference type="UniPathway" id="UPA00575"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004799">
        <property type="term" value="F:thymidylate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006231">
        <property type="term" value="P:dTMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00351">
        <property type="entry name" value="TS_Pyrimidine_HMase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.572.10">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01686">
        <property type="entry name" value="Thymidy_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045097">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023451">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036926">
        <property type="entry name" value="Thymidate_synth/dCMP_Mease_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014620">
        <property type="entry name" value="Thymidylate_synthase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03283">
        <property type="entry name" value="thy_syn_methano"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548">
        <property type="entry name" value="THYMIDYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11548:SF1">
        <property type="entry name" value="THYMIDYLATE SYNTHASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00303">
        <property type="entry name" value="Thymidylat_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036752">
        <property type="entry name" value="TSase_MJ051"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55831">
        <property type="entry name" value="Thymidylate synthase/dCMP hydroxymethylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4 5" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate synthase/dCMP hydroxymethylase" evidence="6">
        <location>
            <begin position="79"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96043.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="209" mass="24449" checksum="86231B8DB0416C0B" modified="2013-06-26" version="1">MIIKKPSVAAAYEELVHAILKHGREVITEDGQKCKELMNVLVEITNPAIKRVSPKYPFGKEAIDKYINNLLYGSNNDFVYDYHERIFKYPSYDRKVVNNQIDYVINKLKNAGTSRRAVISLWQPFIDQENKDVPCLNYINFQKREDSLYMTVVFRSNDILLAFHANALALIRLGELVAEKIGGRLKSYNHFICNAHIYVERDRDYLERW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1K7</accession>
    <name>N6V1K7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2067 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03873</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96163.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591374.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1K7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00405"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.759"/>
    <dbReference type="OrthoDB" id="65057at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019202">
        <property type="entry name" value="DUF2067"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09840">
        <property type="entry name" value="DUF2067"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96163.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="207" mass="24186" checksum="61718370E99E5C2C" modified="2013-06-26" version="1">MKKSFFVKVSCDDELLEICKVLEKAKIDCILESKGNRLKIDVFGYDNESLEENYRTVRAILEKIKRKYNKDKEGFYTYILSELKYPVNKDLIAETLKYLGYKVKYLKDENILKTDVNLKTFENILKSLHEISESIRFSNLGSKPVKNLVIMVSYIKKKSPEEVVEEALREGFFREEEGKVVLNKDINLAKKYFLGDINGDKDIGEER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1W9</accession>
    <name>N6V1W9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase</fullName>
            <ecNumber evidence="2 11">4.3.1.32</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">FO synthase subunit 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">cofG</name>
        <name evidence="13" type="ORF">J422_02964</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the radical-mediated synthesis of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) from 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + S-adenosyl-L-methionine = 7,8-didemethyl-8-hydroxy-5-deazariboflavin + 5'-deoxyadenosine + L-methionine + NH4(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55204"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
            <dbReference type="ChEBI" id="CHEBI:85936"/>
            <dbReference type="EC" id="4.3.1.32"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Cofactor biosynthesis; coenzyme F0 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The FO synthase complex consists of two subunits, CofG and CofH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the radical SAM superfamily. CofG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.32" evidence="2 11"/>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96288.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590739.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1W9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00140"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.581"/>
    <dbReference type="OrthoDB" id="35347at2157"/>
    <dbReference type="UniPathway" id="UPA00072"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044689">
        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042364">
        <property type="term" value="P:water-soluble vitamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01611">
        <property type="entry name" value="FO_synth_sub1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019939">
        <property type="entry name" value="CofG_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03550">
        <property type="entry name" value="F420_cofG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004884">
        <property type="entry name" value="PRK06245.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076:SF15">
        <property type="entry name" value="7,8-DIDEMETHYL-8-HYDROXY-5-DEAZARIBOFLAVIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="FO SYNTHASE (COFH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00294">
        <property type="entry name" value="7_8-didemethyl-8-hydroxy-5-dea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01388">
        <property type="entry name" value="7_8-didemethyl-8-hydroxy-5-dea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01064">
        <property type="entry name" value="F420__menaquinone_cofactor_bio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 11" id="KW-0408">Iron</keyword>
    <keyword evidence="7 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="12">
        <location>
            <begin position="34"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96288.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="359" mass="41745" checksum="062F2BDDE996EC46" modified="2013-06-26" version="1">MKRDEAIEFLKYGDYKDIVYYINIINEKFEREYITYSKNVFIPLSKWCRNRCGYCIFREDIPHLMKPSEVKEILLKGDKLGCREALFTFGEKVDENKEIKKVLNSLGYNNVLEYLYDLEQWCLSNTSLLPHTNCGLLSYDEMKMLREVNASMGLMLENVSERLMKTVAHKNSPGKDPKKRIEMIENAGKLKIPFTTGLLIGIGETEEEIVDSLLKIKELHDKYGHIQEVIIQNFKPKKGTPMENYKEPSPIKMLKVIIVARLILDNTSIQIPPNLNRETGQLFLLAGVDDWGGVSPITKDYVNPEAEWPEIKKLREMTEELGLKLKMRLPVYDKYISEKWLSERVYKKIVGEYGNKKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1Z4</accession>
    <name>N6V1Z4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Archaeal Rqc2 homolog aRqcH</fullName>
            <shortName evidence="2">aRqcH</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">rqcH</name>
        <name evidence="4" type="ORF">J422_03074</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probably part of the ribosome quality control system (RQC). May mediate the addition of alanine residues (Ala tailing) to incompletely synthesized nascent chains from stalled ribosomes, leading to their degradation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Associates with stalled 50S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NEMF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96308.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590759.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1Z4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00162"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.603"/>
    <dbReference type="OrthoDB" id="10943at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990112">
        <property type="term" value="C:RQC complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0072344">
        <property type="term" value="P:rescue of stalled ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.310.10:FF:000003">
        <property type="entry name" value="Zinc knuckle domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.310.10">
        <property type="entry name" value="ibrinogen binding protein from staphylococcus aureus domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00844_A">
        <property type="entry name" value="RqcH_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008532">
        <property type="entry name" value="NFACT_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051608">
        <property type="entry name" value="RQC_Subunit_NEMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043681">
        <property type="entry name" value="RqcH_archaeal"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041120">
        <property type="entry name" value="RqcH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239">
        <property type="entry name" value="NUCLEAR EXPORT MEDIATOR FACTOR NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15239:SF6">
        <property type="entry name" value="RIBOSOME QUALITY CONTROL COMPLEX SUBUNIT NEMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05670">
        <property type="entry name" value="NFACT-R_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05833">
        <property type="entry name" value="NFACT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="NFACT RNA-binding" evidence="3">
        <location>
            <begin position="475"/>
            <end position="579"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="201"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="288"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="410"/>
            <end position="463"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96308.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="666" mass="78071" checksum="0FB61832CE41F99F" modified="2013-06-26" version="1">MKNEITNIDLAAVVEELQMLINGRVDKAFLINNEQNKELILKVHVPEKGSRELVISVGKYKYVTLTNYEREKPKLPPSFAMLLRKHLKNSKITKIEQINFDRILLIEFQNKYKLIAELFKDGNIIFLDENNTIIAPLRIEEWKDRRIAPKENYKFPPQKPLSPKNLEYSLAYELFEEYFIKNSDVECVRLLSRILGIAGIYAEEICERANIDKKKKNLNKEEIKQLFEKAKELFNEVYNNRKPQIIIKDGEYIDVQPIELKKYSDYEKKYYNSFLEAIDDYFAKFLALKEVKKEKSKLEEEIEKHEAILRRQLETLKKYKEESEENSLKGDLIYANYQLVEELLNAIRFARERKSWEEIKKIIRENKNHPILGLIENIDENSGKIVVRLKSEDMEERVALDIRKNAFENAEVYYEKAKKLKSKLEGIENAIELTKKKIEDLKKKSEEEIKEIEEKKMKKKKRKERKWYEKFKWTVINGFLVLAGKDAITNEILIKKYTDKDDIVFHADIQGAPFTVIKTEGREVDEDTLLEVAKFSVSHSKAWKLGYGAIDTYWVKPEQISKTPESGEYLKRGAFVIRGKRNYIRSVPLELGIGVIEYDGEIKITTAPPKTLEKSFIKYVLLKPSNKDKGKVVKELKKIFEDYGIDDEDILMALPPGGCEIIQPKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6V212</accession>
    <name>N6V212_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 19 20">ATP phosphoribosyltransferase</fullName>
            <shortName evidence="19">ATP-PRT</shortName>
            <shortName evidence="19">ATP-PRTase</shortName>
            <ecNumber evidence="7 19 20">2.4.2.17</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19 23" type="primary">hisG</name>
        <name evidence="23" type="ORF">J422_03149</name>
    </gene>
    <organism evidence="23 24">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="23 24" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="23 24">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="18 19">Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 19">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + ATP</text>
            <dbReference type="Rhea" id="RHEA:18473"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="2.4.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="19">Feedback inhibited by histidine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 19">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3 19">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5 19">Belongs to the ATP phosphoribosyltransferase family. Long subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ATP phosphoribosyltransferase family. Short subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="23">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.17" evidence="7 19 20"/>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96323.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590788.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V212"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00176"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.618"/>
    <dbReference type="OrthoDB" id="33116at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00006"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003879">
        <property type="term" value="F:ATP phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13594">
        <property type="entry name" value="PBP2_HisGL4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.120:FF:000002">
        <property type="entry name" value="ATP phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000082">
        <property type="entry name" value="ATP phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00079">
        <property type="entry name" value="HisG_Long"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01018">
        <property type="entry name" value="HisG_Short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020621">
        <property type="entry name" value="ATP-PRT_HisG_long"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013820">
        <property type="entry name" value="ATP_PRibTrfase_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018198">
        <property type="entry name" value="ATP_PRibTrfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001348">
        <property type="entry name" value="ATP_PRibTrfase_HisG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024893">
        <property type="entry name" value="ATP_PRibTrfase_HisG_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013115">
        <property type="entry name" value="HisG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00070">
        <property type="entry name" value="hisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03455">
        <property type="entry name" value="HisG_C-term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403:SF10">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21403">
        <property type="entry name" value="ATP PHOSPHORIBOSYLTRANSFERASE ATP-PRTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01634">
        <property type="entry name" value="HisG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08029">
        <property type="entry name" value="HisG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01316">
        <property type="entry name" value="ATP_P_PHORIBOSYLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 19" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="15 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 19" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 19 23" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="17 19" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="16 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="24" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12 19 23" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP phosphoribosyltransferase catalytic" evidence="21">
        <location>
            <begin position="50"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine biosynthesis HisG C-terminal" evidence="22">
        <location>
            <begin position="214"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00011946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00020998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00024861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="NCBIfam" id="TIGR00070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="Pfam" id="PF01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="22">
        <source>
            <dbReference type="Pfam" id="PF08029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="EMBL" id="ENN96323.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="24">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="289" mass="32185" checksum="98E903EE1E0FA532" modified="2013-06-26" version="1">MKIMFALPNKGRIYEPVMKLLERAGLKITAKSRSLFANTVDENIKVMFARARDIPEFVADGIADIGITGYDLILERGVEDRVEFLLDLNFGYAKLVIAAPEDSGIEKVDDIKDGMKIATEFPNITRKFFEKLNKKVEIIELTGATEIAPFIGVADLISDLTSTGTTLRLNRLKVIEEILTSTTRLIANKNSLNNKYKKEKINKIVLAIKSALYAEKKRLIMMNAPKDKIDEIKKIIPGLAGPTVSEVLSDDNIVAVHAVVDEDEIFNLVPKLHALGARDILIVPIERIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6V294</accession>
    <name>N6V294_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Histidine--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Histidyl-tRNA synthetase</fullName>
            <shortName evidence="10">HisRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">hisS</name>
        <name evidence="13" type="ORF">J422_02934</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>tRNA(His) + L-histidine + ATP = L-histidyl-tRNA(His) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17313"/>
            <dbReference type="Rhea" id="RHEA-COMP:9665"/>
            <dbReference type="Rhea" id="RHEA-COMP:9689"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78527"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.21"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.21" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96393.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590666.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V294"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00135"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.576"/>
    <dbReference type="OrthoDB" id="8659at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004821">
        <property type="term" value="F:histidine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006427">
        <property type="term" value="P:histidyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00773">
        <property type="entry name" value="HisRS-like_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00859">
        <property type="entry name" value="HisRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00127">
        <property type="entry name" value="His_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00125">
        <property type="entry name" value="HisZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015807">
        <property type="entry name" value="His-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041715">
        <property type="entry name" value="HisRS-like_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004516">
        <property type="entry name" value="HisRS/HisZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033656">
        <property type="entry name" value="HisRS_anticodon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004517">
        <property type="entry name" value="HisZ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00442">
        <property type="entry name" value="hisS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00443">
        <property type="entry name" value="hisZ_biosyn_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707:SF1">
        <property type="entry name" value="HISTIDINE--TRNA LIGASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43707">
        <property type="entry name" value="HISTIDYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13393">
        <property type="entry name" value="tRNA-synt_His"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001549">
        <property type="entry name" value="His-tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="6"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="77"/>
            <end position="79"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="259"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="263"/>
            <end position="264"/>
        </location>
        <ligand>
            <name>L-histidine</name>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047639"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001549-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96393.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="405" mass="47550" checksum="F5A4903E879C75B8" modified="2013-06-26" version="1">MFQKPRGTRDFTPEEMKKRRYIEKKLREVFERYGYEEILTPTFESFELIAKKTGEEIRKQLYVFKDYSGRELALRPEMTSSVARFYINELKILQKPLRLYYFANCFRYERPQFGRYREFWQMGCELIGSDKALADAEVINLAIEGLKNINLRFETHISHLGILKGIFEKFNIKEDDEVKIRRLIDKQDFEGLDNLLTSILDDEKKEFIFDILNLKGDVLDELKEMLKDFPKSIDAIINLEEILDYVIYDNYTINLGIARGLDYYTGMVFEIYCNGKQICGGGRYDNLIETFGGEKTPAVGFAYGFDRIMLNVDIKLDEEKYLIVPVRGDKELIKKALIVAKKLRDKGKTVEFEIMGRKLKKALEYANNRNIRYVVIIGEREVKEGKITLKDMKTGEQKILDLEEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2A8</accession>
    <name>N6V2A8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methanogenesis marker 16 metalloprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_02469</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96403.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590438.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2A8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00779"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.483"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002708">
        <property type="entry name" value="HcyBio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017677">
        <property type="entry name" value="Methan_mark_16"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03287">
        <property type="entry name" value="methan_mark_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF3">
        <property type="entry name" value="4FE-4S FERREDOXIN-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01837">
        <property type="entry name" value="HcyBio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="280"/>
            <end position="311"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96403.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="372" mass="41859" checksum="97B83E893C2AF250" modified="2013-06-26" version="1">MKIVTVDKLKKMIRNNEDISEIDIVTTATCGIMSGTMAVFHIPFNDKFRKAEKIYLDGYKGFVGPCPNEYLGKVDAVFFRNGFLFKRLVKGEELEAKVLADNKYYNNIITINDLQKAEMIGTRMCFKNYSAITNLSDKPVKTIFHRLPLKRGEASFSGCGEFNPLENMVIKNEKDLIGKRALLNGADALILGFGTRASLEKPNLMISADMKEMDSYYLGGFLTGDGEEVFNTVAIAVDVKQHIEALKKLDKDIALPLVNIFGREIIDISNYGEVWENVDLRPKLHKDRCKNCKNCLAEEYCPTMAIKKINGEIKISKDCFGCGICSICPHGVFEIKLGSICGIPITCRQSDRKRALDLAKELKKRLEKSFIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2G6</accession>
    <name>N6V2G6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 19 20">Tetrahydromethanopterin S-methyltransferase subunit D</fullName>
            <ecNumber evidence="18 19 20">7.2.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 19">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">mtrD</name>
        <name evidence="21" type="ORF">J422_02175</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 19">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="7.2.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 19">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 19">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Cell membrane</location>
            <topology evidence="2 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the MtrD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.1.4" evidence="18 19 20"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96458.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590243.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2G6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01183"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.427"/>
    <dbReference type="OrthoDB" id="147994at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01097">
        <property type="entry name" value="MtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005779">
        <property type="entry name" value="MeTrfase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01112">
        <property type="entry name" value="mtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04207">
        <property type="entry name" value="MtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016552">
        <property type="entry name" value="MtrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 19" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="9 19 21" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 19" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19 21" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 19" id="KW-1278">Translocase</keyword>
    <keyword evidence="11 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="47"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="77"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="171"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="NCBIfam" id="TIGR01112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ENN96458.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="231" mass="23017" checksum="F408BFF35A0111D8" modified="2013-06-26" version="1">MDIANLIIPLVEITVAGAIINSSVHFVPVGGAPAAMATSTGVGTGTTQLAAGAGFTGLLAAAVMYSKVASLGVIGYILTLLSGAVGSMIMLGVTMLIAQLIYVFGVGVVPAADKCEVDPITKDEQKPYITPGTTGHGIPTVCYISGLIGAALGGIGGGLAYIALASIGFSPAVAGMIAVGFFFINAVLASYNIGGTIEGFHDPKFKKMPNGVIASTVASLLCGIIIILMAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2I5</accession>
    <name>N6V2I5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF5320 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02250</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96473.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590282.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2I5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01198"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.442"/>
    <dbReference type="OrthoDB" id="66053at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="57"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96473.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="11952" checksum="ECCAFF7AA77402D9" modified="2013-06-26" version="1">MFGWGRRFGWWGRGFRRIPIRVGRFTYVGPCRCGFGPHAFYVDDYGRLVHAWELPYYVSTEEDKSLIEERIKDLEMEKKMLEEEIMRLKKELERSKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2J8</accession>
    <name>N6V2J8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Haloacid dehalogenase domain-containing protein hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02310</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96483.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590299.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2J8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01209"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.454"/>
    <dbReference type="OrthoDB" id="8588at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005507">
        <property type="term" value="F:copper ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043682">
        <property type="term" value="F:P-type divalent copper transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055070">
        <property type="term" value="P:copper ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520">
        <property type="entry name" value="ATP7, ISOFORM B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43520:SF8">
        <property type="entry name" value="P-TYPE CU(+) TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00119">
        <property type="entry name" value="CATATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-1278">Translocase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96483.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="268" mass="29940" checksum="455693BDE1475D04" modified="2013-06-26" version="1">MKIAVVFDSAGTLVKIIRVIKDLKRGKFICNRQTVDIVDEKVGRALVIIKDDPLKTVDVCDDNKLITDFLKEVDIGISYCNPPIDINGIYKDKKAKMKELKEPLEILKKYNIETGYGSALIVDTNEGEIEYTIATAGCLFPEVKDTIKELKNLGVKVFIASGDRREFIEKLAELTGVNKRYILPEAHHKLKKDLIKNLKTDGYFTIMVGDGANDVPAMKESHLAIATLQSGYTSKKAIEAADIKIENIKEVVDIVKKLLEEKNEAKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2N1</accession>
    <name>N6V2N1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ribonuclease Z</fullName>
            <shortName evidence="9">RNase Z</shortName>
            <ecNumber evidence="9">3.1.26.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA 3 endonuclease</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNase Z</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rnz</name>
        <name evidence="11" type="ORF">J422_01820</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Zinc phosphodiesterase, which displays some tRNA 3'-processing endonuclease activity. Probably involved in tRNA maturation, by removing a 3'-trailer from precursor tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Endonucleolytic cleavage of RNA, removing extra 3' nucleotides from tRNA precursor, generating 3' termini of tRNAs. A 3'-hydroxy group is left at the tRNA terminus and a 5'-phosphoryl group is left at the trailer molecule.</text>
            <dbReference type="EC" id="3.1.26.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 2 Zn(2+) ions.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the RNase Z family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.11" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96513.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590119.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2N1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00213"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.358"/>
    <dbReference type="OrthoDB" id="85118at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042781">
        <property type="term" value="F:3'-tRNA processing endoribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07717">
        <property type="entry name" value="RNaseZ_ZiPD-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.15.10:FF:000002">
        <property type="entry name" value="Ribonuclease Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01818">
        <property type="entry name" value="RNase_Z_BN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013471">
        <property type="entry name" value="RNase_Z/BN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000801">
        <property type="entry name" value="PRK00055.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02651">
        <property type="entry name" value="RNase_Z"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46018:SF2">
        <property type="entry name" value="ZINC PHOSPHODIESTERASE ELAC PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23023">
        <property type="entry name" value="Anti-Pycsar_Apyc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="8 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="10">
        <location>
            <begin position="17"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="63"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96513.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="310" mass="35580" checksum="70950E23697AAE34" modified="2013-06-26" version="1">MLTFLGTSAAVPTKRRNHIGIALKYDGDVFLFDCGENIQRQMLSTKVSPMRIKSIFITHLHGDHILGLPGLLQSLAFMGRKNKLKIYGPEGIEDIVRMSLNFGYFVREYDIEVITVKTDNPKIIEKTEKYEIIAYPTKHSIESYGYIFKEIKKPRLNPEKAKEFGVRKEDFKKLKNGIPVKSIYGLTVYPYQVLLPPKKGMCLAYSGDTLPIEDFGRYLKNLGCDFLIHEATFDDNLKDEAIETMHSTIGDAVNIAKIAGVKVLFLTHISARYDKEYFNIYKSNAEKYSNEKFKIFIPEDLESYNLKDFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6V342</accession>
    <name>N6V342_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Glucose-6-phosphate isomerase</fullName>
            <ecNumber evidence="7">5.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_01211</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>alpha-D-glucose 6-phosphate = beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:11816"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:58225"/>
            <dbReference type="EC" id="5.3.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GPI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.9" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589908.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V342"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00802"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.237"/>
    <dbReference type="OrthoDB" id="168618at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00181"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004347">
        <property type="term" value="F:glucose-6-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048029">
        <property type="term" value="F:monosaccharide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051156">
        <property type="term" value="P:glucose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05015">
        <property type="entry name" value="SIS_PGI_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001672">
        <property type="entry name" value="G6P_Isomerase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053509">
        <property type="entry name" value="GPI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018189">
        <property type="entry name" value="Phosphoglucose_isomerase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035476">
        <property type="entry name" value="SIS_PGI_1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040629">
        <property type="entry name" value="PGI_Meth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11469:SF1">
        <property type="entry name" value="GLUCOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00342">
        <property type="entry name" value="PGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00662">
        <property type="entry name" value="G6PISOMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00765">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00174">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51463">
        <property type="entry name" value="P_GLUCOSE_ISOMERASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="4" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00029321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="385" mass="44709" checksum="7D05833FCA6810FC" modified="2013-06-26" version="1">MINFNYENILKLNEIDIKFDYKEYYNRFLNKLENTVGFRKAIFEDINKYKIEKAEDVVVIGMGGSILGTETVYYALKGYNKLNNAYFLDNTDPEKIVKIINEIKDKEPLIYVVSKSGNTLETIVNYNLIKKEVKGDYIFITNGGKLLEEAKEKGYYIMNIPENVPGRYSIFTAVGLAPLYSLNVNIDRLINGAKYMDKIFLEEDFYKNYPIINAIIHYLYEKRGVDIAVLMSYVESLYHFGEWFKQLLGESLGKEGKGITPLLSIGAKDQHSLLQLYMDGKKDKIITFLTVEKYRDDIEVEYNNKIFKLSEIIKYEQLATEKALTNNKVPNVKITLEEVKEECLGALLYMYMLQIGFMGELYNINAYNQPAVEEEKRICLEMMMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3F2</accession>
    <name>N6V3F2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Beta-lactamase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_00030</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589699.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3F2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01161"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.6"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06262">
        <property type="entry name" value="metallo-hydrolase-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051453">
        <property type="entry name" value="MBL_Glyoxalase_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46233">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE GLOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46233:SF3">
        <property type="entry name" value="HYDROXYACYLGLUTATHIONE HYDROLASE GLOC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="5">
        <location>
            <begin position="11"/>
            <end position="180"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="196" mass="22425" checksum="F72A61B50708B51F" modified="2013-06-26" version="1">MIVKLGGFGYSCNTYLILDKYHILIDPGMYANIIDEIEKYTSKIDFVINTHCHYDHVGVDHLIEEIYNCPIYIDDKEVRHLKNADHVVLAELFNSKLIPPKEVLPISELEDVEIIRTPGHTYGSITILYRDGLITGDTLFAYGVGRTDFPTGDIIKLRESIAMLEKIANQRDIKHIYPGHGEIGDRRAFQYAKLFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3H6</accession>
    <name>N6V3H6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS7</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps7</name>
        <name evidence="11" type="ORF">J422_00150</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the head domain of the 30S subunit. Is located at the subunit interface close to the decoding center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7 9">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS7 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589719.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3H6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01138"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.30"/>
    <dbReference type="OrthoDB" id="45346at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14867">
        <property type="entry name" value="uS7_Eukaryote"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.455.10">
        <property type="entry name" value="Ribosomal protein S7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00480_A">
        <property type="entry name" value="Ribosomal_uS7_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000235">
        <property type="entry name" value="Ribosomal_uS7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026018">
        <property type="entry name" value="Ribosomal_uS7_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020606">
        <property type="entry name" value="Ribosomal_uS7_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023798">
        <property type="entry name" value="Ribosomal_uS7_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036823">
        <property type="entry name" value="Ribosomal_uS7_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005716">
        <property type="entry name" value="Ribosomal_uS7_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003106">
        <property type="entry name" value="PRK04027.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01028">
        <property type="entry name" value="uS7_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11205">
        <property type="entry name" value="RIBOSOMAL PROTEIN S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00177">
        <property type="entry name" value="Ribosomal_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002122">
        <property type="entry name" value="RPS7p_RPS7a_RPS5e_RPS7o"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47973">
        <property type="entry name" value="Ribosomal protein S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00052">
        <property type="entry name" value="RIBOSOMAL_S7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS7" evidence="10">
        <location>
            <begin position="25"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00480"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="191" mass="21808" checksum="6478F92B2C11A685" modified="2013-06-26" version="1">MELDEIKIFGKWSTKDVVVRDPGLRNYINLTPIYVPHTAGRFTKRQFEKHKMNIVERLVNKVMRREENTGKKLKALKIVEEAFEIIEKRTKQNPIQVLVDAIENAGPREDTTRIQYGGIIYLQSVDCSSSRRVDVALRNIALGAYMKAHKSKTPIEVALAEEIIAAAKGDIQKSYAVRKKEETERVAQSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP04</accession>
    <name>N6VP04_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycosyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_07162</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000077">
        <property type="protein sequence ID" value="ENN95565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP04"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008713">
        <property type="term" value="F:ADP-heptose-lipopolysaccharide heptosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051199">
        <property type="entry name" value="LPS_LOS_Heptosyltrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30160:SF1">
        <property type="entry name" value="LIPOPOLYSACCHARIDE 1,2-N-ACETYLGLUCOSAMINETRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30160">
        <property type="entry name" value="TETRAACYLDISACCHARIDE 4'-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="56" mass="6354" checksum="71ED7A9E94097D41" modified="2013-06-26" version="1" fragment="single">MKILLFKIGAIGDALMTTPLIRQLRKNFPNAQIDYLIGKHAHAVLEGNIYLNNLYN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="14" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP63</accession>
    <name>N6VP63_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyl-accepting chemotaxis sensory transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06748</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000058">
        <property type="protein sequence ID" value="ENN95645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP63"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="33"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="73" mass="8089" checksum="82BE8A3439410064" modified="2013-06-26" version="1">MQESAKTSKENVETALRHIQDIASISEEFAATAEELTASAEEMSRIVEEISKATEEISEISSRVSKEAEKFII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPD2</accession>
    <name>N6VPD2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Signal transduction protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_06269</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593357.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPD2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00284"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1221"/>
    <dbReference type="OrthoDB" id="65817at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="75"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="162" mass="18227" checksum="91A91281A9DFEEBF" modified="2013-06-26" version="1">MVATIPVLVIMKKPVIVSGDVSIDKIAKIMIDENIPCVLVIKKDNSIEKVDEEDIIKKVIIKKLSPEKVKTEDISSPKIIKVHKNTPVEEAMEIMSKNKVKELFVVDDDGKIIGMITEEDVVNVAPDIINTLESLINHLLEIVDEVLEEEKKEKKIVRIPIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPK3</accession>
    <name>N6VPK3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methyltransferase type 12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05798</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592825.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPK3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01246"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1130"/>
    <dbReference type="OrthoDB" id="182741at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="363" mass="42816" checksum="99A96A6064370CB5" modified="2013-06-26" version="1">MKFDLTGEEKCNREEIVSSSLSDIINEVIGDVVNSELKVRKKIEMLNDTIKYLYYELLGTFIKQGIEFGIFPNIAKYKPKFEEIPNVIKYPNKEFIIDFIKTAIKLNILEYKDDERLDINEFYKLEIKLPKMDNIISDYIMKFNYVTHVARYALISYNHPKIALSFKKDPDIWDMILSSPYYEFHREIAANYLDIEEDDYILDIGCGSRSPQFFIDNVYPKGYYTGVDISKGLLEMAEFRVKNIYPSGYELKNLDFLEVIPKRKYNKIICSHVLKYIPSLKIFIRKLAKSLAPGGKVYISEEFIPKYANDKKDYCEIFGFYNRLNRKFVKYYSEEEIKKELELLGDYRIKRLSDGIIVIENVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPK7</accession>
    <name>N6VPK7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type II secretion system protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_05823</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592834.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPK7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01241"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1135"/>
    <dbReference type="OrthoDB" id="33500at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01130">
        <property type="entry name" value="VirB11-like_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.450.370:FF:000001">
        <property type="entry name" value="Secretion system protein E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:002252">
        <property type="entry name" value="Type IV secretion system protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.390.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF14">
        <property type="entry name" value="FLAGELLA ACCESSORY PROTEIN I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial type II secretion system protein E" evidence="2">
        <location>
            <begin position="171"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="547" mass="63178" checksum="3B6A32C6E3F1984E" modified="2013-06-26" version="1">MAEAELREAMNRNPHLRRYIENFKRVYLKVPDFMVSLSRELRELKYPNIIYPVGDPIFIHIFGTPETKTKYIVIEPRLETPEERLKYKMILNKILELAPYEKTPESNDEFEEILVRLFNMATKVVEPTEKESFITRVFKFTDNRVRITPEERSKFLYLLKRDLIGLGVLEPISRDPYLEDIHVIGAKNCHVVHKIFGMLPTNIVWEDDIELADYLKNIGERMGRPVSDARPIVDGALPDGSRINIIYSTDVSLKGPSFTIRKFTEVPISVTQLISWGTFSAEIAAYLWLCLEYGMSIFICGETASGKTTTLNAILPFIKPNSKIFSCEDTPEVKPPHPVWQQLITRERGPEESRVTLFDLLRAALRSRPNYIIVGEIRSVEAAVAFQAMQTGHPVLSTFHAANVRKMIQRLTGEPINVPITFIDNLNVALFQLAVYQRGKVLRRVVTVEEIEGYYKEVDGVITRAVFQWEPNKDRHTFTGKNNSYILEEKIAKAAGYDDPREIYNELELRAKILEEMIAREIFDYYQVRDIIWAFYEKGLEGLPFSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ83</accession>
    <name>N6VQ83_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04183</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96040.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ83"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00685"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96040.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="26" mass="3052" checksum="71A753C0D0F5FBFB" modified="2013-06-26" version="1">MIKRIELKNFKSFKNLSLELPNGFIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ88</accession>
    <name>N6VQ88_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Elongation factor Tu domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_04208</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96045.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ88"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00680"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.823"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03696">
        <property type="entry name" value="SelB_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000020">
        <property type="entry name" value="Translation elongation factor EF-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050055">
        <property type="entry name" value="EF-Tu_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721">
        <property type="entry name" value="ELONGATION FACTOR TU-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43721:SF11">
        <property type="entry name" value="SELENOCYSTEINE-SPECIFIC ELONGATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="4" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation elongation factor EFTu-like" evidence="5">
        <location>
            <begin position="154"/>
            <end position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96045.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="307" mass="34905" checksum="8EA78D600E01F612" modified="2013-06-26" version="1">MITVGIFGHIKDVGRELGKKGTSSDITFYNYKQGDKIVTYVEPTRYPERINPLIYTINLMDYAIVFIDEITGELGEMLLALDMFNICNGAFVLGEYVDVDMLKNIIKGTSMENFDILDKDFIKIREHVLELEIERDFSYKKIPIDHYFKVKSVGTVVLGKVEKGKIEVHDNLKLYPIYKEVMIKSIQIHDKDCKEAKAGDRVGLALKGVDVEELSRGYVLSDKELEVKKEVKFKIEMNPFINKEIKEGENYTLITGLQAVACEVLDVSNNKISLLLNKEIAYDDEKLCLIDGSAKIRILGVGEKLCQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQE9</accession>
    <name>N6VQE9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NGG1p interacting factor 3 protein, NIF3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_04488</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GTP cyclohydrolase I type 2/NIF3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96095.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591823.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQE9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00627"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.880"/>
    <dbReference type="OrthoDB" id="85198at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1390.30:FF:000001">
        <property type="entry name" value="GTP cyclohydrolase 1 type 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1390.30">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002678">
        <property type="entry name" value="DUF34/NIF3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036069">
        <property type="entry name" value="DUF34/NIF3_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00486">
        <property type="entry name" value="YbgI_SA1388"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799:SF14">
        <property type="entry name" value="GTP CYCLOHYDROLASE 1 TYPE 2 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13799">
        <property type="entry name" value="NGG1 INTERACTING FACTOR 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01784">
        <property type="entry name" value="DUF34_NIF3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102705">
        <property type="entry name" value="NIF3 (NGG1p interacting factor 3)-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR602678-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96095.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="239" mass="26754" checksum="947E22FB057887A1" modified="2013-06-26" version="1">MNVYEIIEIIENLAPTEMAIEGDNVGLQVGEDREVNKLGVALDPSLEVIKKAVKEGVDFLFTHHPLLKDPINKFTGVVYKKLELLINNKIVLYSAHTNLDVAFLNDALSELFNLKNIQPLYDNGLGRVGIFEGTFKELLDIVKENLCKNPIVVNPKDIENFKLAVLSGYGLSQKSIIHAAKNSDVYISGDLTHHARILAEDMNFSVIDATHYGTEVYGLKKFKNILEEKLDVEIISLDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQF7</accession>
    <name>N6VQF7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">S-layer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_04548</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96105.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591844.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQF7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00616"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.892"/>
    <dbReference type="OrthoDB" id="92388at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.98.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022651">
        <property type="entry name" value="S_layer_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022650">
        <property type="entry name" value="S_layer_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006454">
        <property type="entry name" value="S_layer_MJ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01564">
        <property type="entry name" value="S_layer_MJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05124">
        <property type="entry name" value="S_layer_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05123">
        <property type="entry name" value="S_layer_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-layer protein outer" evidence="2">
        <location>
            <begin position="35"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="domain" description="S-layer protein central" evidence="1">
        <location>
            <begin position="114"/>
            <end position="452"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF05124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96105.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="543" mass="58393" checksum="A98F51E9FED6863C" modified="2013-06-26" version="1">MAISLKKIGAIAVAGAMLGTAVASGVAAQVTIIGDLTKDLFVKDGQPNCYVVVGANAPSTMDVVSAADIAAKIGSLCYKEANVEDGSAEIEVSAKADSDDVDIYTNGIPNGNATIFVAAADSDYSDNFATTTANINDTNSNVFDDTQVKNVKSLGDVATLMKIDDVDPSDWYNSDDDAAEVVFVKLENNSGSLTVDKKDAVYASIVYKDDLSTFKDTTTLKVGYRIPFLGEEYAVVKIDNDNDAIYIGKPVYDGVLKEGETYDLGNGYEVKVKAILTDNTNYKADVQILKDGKVVAEKFDTTPLKIIYNDIGVVVNKAWQDIPGNYGFAEVLITNNVKELDLGKEYVKDWKLYAVVNNSGTMELTDQLPPSSGNVVGIALRYEGDKLDDLDSGDSIAFPNNYVEFKLDDKDKKNKLYVYYEMDESKDTTLNIGEKTKVLNADIKLKGIKAEAQQVVPVTAPIAKLDTEVSLDNADRNLVLVGGPVANKLTKELVDEGKINLDNTSPATIAVIKGAANGHDVVVVAGGDREKTREAAEELLKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQP2</accession>
    <name>N6VQP2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Nucleoside transporter/FeoB GTPase Gate domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_03693</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96215.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591280.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQP2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01367"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.721"/>
    <dbReference type="OrthoDB" id="51620at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038880">
        <property type="entry name" value="MJ0871-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38139">
        <property type="entry name" value="GATE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38139:SF1">
        <property type="entry name" value="NUCLEOSIDE TRANSPORTER_FEOB GTPASE GATE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="49"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="175"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="215"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="284"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="Nucleoside transporter/FeoB GTPase Gate" evidence="2">
        <location>
            <begin position="12"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96215.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="305" mass="34434" checksum="4E86B2FE37DF8135" modified="2013-06-26" version="1">MVIVESLLYTIKISSVSIPTVFIFSYLINAGMLDKLSKILHPILRYLNVNPISISSILSCFFSPTVGYSILAEAYRERKIDENVIIATSLANSFPSILSHTLTFFIPIVIPILGFTGLIFILIRIFVSLVKSIIGFIYLTKVKIDFNENVEYKKLNRGECFNLAINRTLKFTKRFVPIMFITLTIVLYLNEIKFFDIIKNLLYPITKFLNLNPNVIVLALSEFINAQSAIILAGEFLKNNILTQKEILIGLIIGNVVTFSTRYVKHSLPLHISLFGVKLGSKIVLINAVITLILDIFIIIILLTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR21</accession>
    <name>N6VR21_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Probable deoxyhypusine synthase</fullName>
            <shortName evidence="11">DHS</shortName>
            <ecNumber evidence="6 11">2.5.1.46</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">dys</name>
        <name evidence="12" type="ORF">J422_02704</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 11">Catalyzes the NAD-dependent oxidative cleavage of spermidine and the subsequent transfer of the butylamine moiety of spermidine to the epsilon-amino group of a specific lysine residue of the eIF-5A precursor protein to form the intermediate deoxyhypusine residue.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>[eIF5A protein]-L-lysine + spermidine = [eIF5A protein]-deoxyhypusine + propane-1,3-diamine</text>
            <dbReference type="Rhea" id="RHEA:33299"/>
            <dbReference type="Rhea" id="RHEA-COMP:10143"/>
            <dbReference type="Rhea" id="RHEA-COMP:10144"/>
            <dbReference type="ChEBI" id="CHEBI:29969"/>
            <dbReference type="ChEBI" id="CHEBI:57484"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82657"/>
            <dbReference type="EC" id="2.5.1.46"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4 11">Protein modification; eIF5A hypusination.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 11">Belongs to the deoxyhypusine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.46" evidence="6 11"/>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96355.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590590.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR21"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00093"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.530"/>
    <dbReference type="OrthoDB" id="17730at2157"/>
    <dbReference type="UniPathway" id="UPA00354"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034038">
        <property type="term" value="F:deoxyhypusine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.910.10:FF:000010">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.910.10">
        <property type="entry name" value="Deoxyhypusine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00153">
        <property type="entry name" value="DHS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022899">
        <property type="entry name" value="Deoxyhypus_synthase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002773">
        <property type="entry name" value="Deoxyhypusine_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036982">
        <property type="entry name" value="Deoxyhypusine_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00321">
        <property type="entry name" value="dhys"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003052">
        <property type="entry name" value="PRK03971.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11703:SF0">
        <property type="entry name" value="DEOXYHYPUSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01916">
        <property type="entry name" value="DS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 11" id="KW-0386">Hypusine biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0520">NAD</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11 12" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00009892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96355.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="332" mass="37866" checksum="C3DFF1A070BB4CDC" modified="2013-06-26" version="1">MDPKDIVLKQSEDISGIEIEGPWLDEDISLEDIIKNYYKKIGFQASHLGKAIEIWKKIEEKRKRGEDIKVFFGYTSNIVSSGLREIIAYLAKHKKVDVIVTTAGGVEEDFIKCLKPFILGDWFVNGKELREKGINRIGNIFVPNDRYIEFEKYMMEFFEELLNRDNKIVTASEFCYKLGEFMDKKLGKEKEKSILYWAYKNNIPIFCPAITDGSIGDMLYFFKKFKKDSELKIDIANDIVKLNDIAINAKETACIILGGSLPKHAIINANLFREGTDYAIYITTALPWDGSLSGAPPEEGISWGKISAEANYVEIWGEATIIFPILVYCVMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR32</accession>
    <name>N6VR32_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_07122</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000074">
        <property type="protein sequence ID" value="ENN95576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594974.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR32"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01212"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1378"/>
    <dbReference type="OrthoDB" id="66039at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58100">
        <property type="entry name" value="Bacterial hemolysins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="27"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="94"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="173" mass="20151" checksum="3C5EE0ECDE4CBE7C" modified="2013-06-26" version="1">MKNDIPLNLSNSINYLINSVKEFRKGNEEMLSLIKQLSNVLDNVEKTLNIIEDKLLIIIERQKSGKEINHYVLEKFVENIENLSHVLENVDKISRSLNLEIEKHESSINNLEDTIEKLKDIDAKISKNVELELKRIHEVIDTNRSELKYISDRCDALNERLKDLLQEIDSLIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR59</accession>
    <name>N6VR59_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rps15p</name>
        <name evidence="4" type="synonym">rps15</name>
        <name evidence="8" type="ORF">J422_06837</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uS15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594240.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR59"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01080"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1326"/>
    <dbReference type="OrthoDB" id="6533at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070181">
        <property type="term" value="F:small ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00353">
        <property type="entry name" value="Ribosomal_S15p_S13e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.10:FF:000003">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.860.130:FF:000001">
        <property type="entry name" value="40S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.860.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.10">
        <property type="entry name" value="S15/NS1, RNA-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01343_A">
        <property type="entry name" value="Ribosomal_uS15_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000589">
        <property type="entry name" value="Ribosomal_uS15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023029">
        <property type="entry name" value="Ribosomal_uS15_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012606">
        <property type="entry name" value="Ribosomal_uS15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009068">
        <property type="entry name" value="uS15_NS1_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006331">
        <property type="entry name" value="PRK08561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885">
        <property type="entry name" value="RIBOSOMAL PROTEIN S15P/S13E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11885:SF6">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08069">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00312">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01386">
        <property type="entry name" value="Ribosomal_S13_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01387">
        <property type="entry name" value="Ribosomal_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47060">
        <property type="entry name" value="S15/NS1 RNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00362">
        <property type="entry name" value="RIBOSOMAL_S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS15 N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="1"/>
            <end position="18"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01386"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="153" mass="17900" checksum="03B353069CCE3203" modified="2013-06-26" version="1">MARMHARKRGKSGSKRPPRKEVPEWVKYTPEEVENLVVELAKKGYQSAQIGLILRDSYGIPDVKLITGKKISRIMKEHGLYPKVPEDLLNLMRRAVNLRKHLEQHPKDLHSKRGLQLIESKIKRLVKYYKRKGVLPQDWRYTPETARLLVEQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRB4</accession>
    <name>N6VRB4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Restriction endonuclease type IV Mrr domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06396</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593525.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRB4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01295"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1245"/>
    <dbReference type="OrthoDB" id="34330at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003509">
        <property type="entry name" value="UPF0102_YraN-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02021">
        <property type="entry name" value="UPF0102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="12628" checksum="671610EC98BF1238" modified="2013-06-26" version="1">MRRGKKKEGMAVNYLKNKGYEILGRNVIKRLNQHKKAEYDIIAKRGRYCYAVEVKSGKQVLTSSDIEKLHKKANKIKAKGLLITGRYVKLTDKAKKELKKRKIRWIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRD2</accession>
    <name>N6VRD2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06506</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593563.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRD2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01274"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1266"/>
    <dbReference type="OrthoDB" id="52832at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004454">
        <property type="entry name" value="HD-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00295">
        <property type="entry name" value="TIGR00295 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38659:SF2">
        <property type="entry name" value="HDIG DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38659">
        <property type="entry name" value="METAL-DEPENDENT PHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="1"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="135" mass="15217" checksum="2CAC6A584DF3E75F" modified="2013-06-26" version="1" fragment="single">IKNNDYNVDLNLVKIGGLLHDIGRSKTHGIDHGVVGANILRSYGFDERIALIAERHIGAGITKEEAIALGLPAKDYVPISLEEKIVAHADNLIFETERVEIDRVVEKFRNKLGKDHPSVKRIIFLDMEINRLLRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRD7</accession>
    <name>N6VRD7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06316</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000051">
        <property type="protein sequence ID" value="ENN95721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRD7"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1229"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42873">
        <property type="entry name" value="RIBOSOMAL RNA LARGE SUBUNIT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42873:SF1">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="95" mass="10568" checksum="E403577446797D9C" modified="2013-06-26" version="1" fragment="single">DPPAFAQSKKDLKSALRGYHMLNRFGATLSNRYLITCSCSQPVEPDAFKALVIDACLKAKKWGKIIIQGSQAPDHPITSKGTEYLKCLFLRVDEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRF2</accession>
    <name>N6VRF2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Bifunctional protein GlmU</fullName>
            <ecNumber evidence="5">2.3.1.157</ecNumber>
            <ecNumber evidence="6">2.7.7.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">J422_02235</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N-acetyl-alpha-D-glucosamine 1-phosphate + UTP + H(+) = UDP-N-acetyl-alpha-D-glucosamine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13509"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:57705"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="EC" id="2.7.7.23"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>alpha-D-glucosamine 1-phosphate + acetyl-CoA = N-acetyl-alpha-D-glucosamine 1-phosphate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13725"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57776"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="EC" id="2.3.1.157"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; N-acetyl-alpha-D-glucosamine 1-phosphate from alpha-D-glucosamine 6-phosphate (route II): step 2/2.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Nucleotide-sugar biosynthesis; UDP-N-acetyl-alpha-D-glucosamine biosynthesis; UDP-N-acetyl-alpha-D-glucosamine from N-acetyl-alpha-D-glucosamine 1-phosphate: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the transferase hexapeptide repeat family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the N-terminal section; belongs to the N-acetylglucosamine-1-phosphate uridyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.157" evidence="5"/>
    <dbReference type="EC" id="2.7.7.23" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96470.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590276.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRF2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01195"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.439"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="UniPathway" id="UPA00113">
        <property type="reaction ID" value="UER00532"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019134">
        <property type="term" value="F:glucosamine-1-phosphate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003977">
        <property type="term" value="F:UDP-N-acetylglucosamine diphosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006048">
        <property type="term" value="P:UDP-N-acetylglucosamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05636">
        <property type="entry name" value="LbH_G1P_TT_C_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04181">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.10.10">
        <property type="entry name" value="Hexapeptide repeat proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023915">
        <property type="entry name" value="Bifunctiontional_GlmU_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050065">
        <property type="entry name" value="GlmU-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056729">
        <property type="entry name" value="GMPPB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011004">
        <property type="entry name" value="Trimer_LpxA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03992">
        <property type="entry name" value="Arch_glmU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584:SF8">
        <property type="entry name" value="N-ACETYLMURAMATE ALPHA-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43584">
        <property type="entry name" value="NUCLEOTIDYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25087">
        <property type="entry name" value="GMPPB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51161">
        <property type="entry name" value="Trimeric LpxA-like enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 17" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="15">
        <location>
            <begin position="3"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-1-phosphate guanyltransferase C-terminal" evidence="16">
        <location>
            <begin position="257"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF25087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN96470.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="408" mass="45969" checksum="7AA0C4440F291AF6" modified="2013-06-26" version="1">MDAVILAAGKGERLRPLTENRPKPLLPIAGKPILEHIIEKIYDKVDNIYVVVKYKREKIIEHFNNYDKIVFLNQGDLEGTGEALLTAENYISDEFIVINGDIVFEDSLDEILNYDYAIAVKKVNNPENFGVFLVDEKNNIIDLEEKPKFPKSNLINAGIYKLKRDIFKYLKEIPISERGEKELTDAIKLLIKEKEVKAIELKGYWNDIGYPWSLLEANKYFLDKIETKIDGEIEENVVIKGKVIIEKGAKIRANSVIEGPAIIKSGAIVGPLAYIRPYTVLMENTFVGNSSEVKGSIIMRNTKIPHLSYVGDSIIGENCNFGCNTITANLRFDNKPVKVNIKGKRVESVRKLGVIMGDNVKTGVQVSFMPGVKVGSNCWIGANCLIDRDVEKNSFIYKKDSLIIKKLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRF9</accession>
    <name>N6VRF9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">uroporphyrinogen-III C-methyltransferase</fullName>
            <ecNumber evidence="2">2.1.1.107</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">S-adenosyl-L-methionine:uroporphyrinogen III methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_02260</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the two successive C-2 and C-7 methylation reactions involved in the conversion of uroporphyrinogen III to precorrin-2 via the intermediate formation of precorrin-1. It is a step in the biosynthesis of both cobalamin (vitamin B12) and coenzyme F430.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>uroporphyrinogen III + 2 S-adenosyl-L-methionine = precorrin-2 + 2 S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32459"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57308"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:58827"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.1.1.107"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:32460"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.107" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96475.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590284.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRF9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01200"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.444"/>
    <dbReference type="OrthoDB" id="24444at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004851">
        <property type="term" value="F:uroporphyrin-III C-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11642">
        <property type="entry name" value="SUMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1010.10:FF:000001">
        <property type="entry name" value="Siroheme synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004790">
        <property type="entry name" value="PRK06136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF3">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT UROPORPHYRINOGEN III METHYLTRANSFERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10 12" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="6" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="11">
        <location>
            <begin position="3"/>
            <end position="207"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00052569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00058927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00075570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96475.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="232" mass="25503" checksum="F8C706FEC5A0EC42" modified="2013-06-26" version="1">MGKVILVGAGPGDKELITVKGLKAIKEADVIVYDDLIDKDLLNYNKNAELIYVGKRKGRHSYKQEDINKILIEKAKEGKTVVRLKGGDPFIFGRGGEEILALKEHNIPYEIIPGLSSATALPALAEIPLTHRKVARSFTVLTAHIEDGEVDFTKFNGDTLVILMGITKLKKIVNDLLKVRDKKTPIAIILKGATKEQKVIRGTLEDILDKAKNIEPPGIIVVGEVVNILQKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRJ7</accession>
    <name>N6VRJ7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Class III signal peptide-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05963</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593043.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRJ7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00566"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1161"/>
    <dbReference type="OrthoDB" id="65764at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="117" mass="13394" checksum="B39A41CF428F7317" modified="2013-06-26" version="1">MKAQISLEFTFVFFVILLTSLITIANFLSKNFTIEDWEIDKIDNAAKTAVMLINSRYEGVCTNTTLIYSGIKWNKGNKVISIYISPRDAVDDRIRDFILSYIENNTNIEGYNITVNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRM8</accession>
    <name>N6VRM8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_05728</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592798.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRM8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01259"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1117"/>
    <dbReference type="OrthoDB" id="50379at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3720.10:FF:000003">
        <property type="entry name" value="Aliphatic sulfonate ABC transporter permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30151:SF0">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN MJ0413-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30151">
        <property type="entry name" value="ALKANE SULFONATE ABC TRANSPORTER-RELATED, MEMBRANE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="160"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="54"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="250" mass="27554" checksum="54CE789E4574D805" modified="2013-06-26" version="1">MIKMIMPFSLIIMWEVLAIYINNPILLPKVEDVIYVLSHPLTNILGTGSLIDNTVVSIKRVLSGFILASTVAVPLGIFMGYSKIIYDLFDTVIELLRPIPPLAWVPLSLVWFGLSESSMIFIIFIGAFFPILINTIAGVRGVPNALVEMALTLGAKKRDILLKIIIPASSPSILTGLRVGAGIAWMCVVAAEMLPSSNSGLGYLIMYAYSLSRMDVVIACMIIIGLIGIVLDNGLRYIENKYFIWRKMMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRP2</accession>
    <name>N6VRP2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Transcriptional regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05803</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592826.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRP2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01245"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1131"/>
    <dbReference type="OrthoDB" id="55560at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010863">
        <property type="entry name" value="EarA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07381">
        <property type="entry name" value="EarA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="112" mass="13130" checksum="5CB6E60CC00D7827" modified="2013-06-26" version="1">MSSLIFIDPMIIRSLNKSKLRKKILYLLYKIYPYGMYLSEISRRVKSDPSNVLGCLRGLNGRYNGHFSLIELGLVECVEKDGIKIYKLTEYGKKVVEFLKEQDSEFIKSLRW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRQ9</accession>
    <name>N6VRQ9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fomrate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_02060</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FrhB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590197.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRQ9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00257"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.404"/>
    <dbReference type="OrthoDB" id="35334at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052592">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="7-HYDROXYMETHYL CHLOROPHYLL A REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332:SF6">
        <property type="entry name" value="FORMATE DEHYDROGENASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="7">
        <location>
            <begin position="320"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="363" mass="41396" checksum="0CE43D6E3B3EDC20" modified="2013-06-26" version="1">MSKYVLAKATNEEILKRAECGGAVTALFKYLLDKKLVDGVLTLKKGEDIYDGIPVFITNSEELIETAGSLHCTSTNFGKIIKNYFLDKKIAVSVKPCDAMAIRELAKLNQINLDNIYMIGLNCGGTINPIKAMKMIKLFYKVDPKDVVKEEIDKGKFIIKLKNGKYKAIKIDKLEEKGFGRRKNCQRCELKIPRNADLACGNWGAEKGWTFIEICSEKGRNLVENAEKDGYIKIKEPSKKNIEIRKKIEENMIKLAKIFQKRHFEESYISIEEWQKYWNRCIKCYGCRDACPICICNNCKLEFISENGKIPPDPAIFHGIRLSCVSKNCINCGQCEDVCPMEIPLAYITHRVYLKIKKALYLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRW4</accession>
    <name>N6VRW4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methanogenesis marker 13 metalloprotein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_01353</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the NifD/NifK/NifE/NifN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589934.1">
        <property type="nucleotide sequence ID" value="NZ_AQUK01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRW4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00018"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.265"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017675">
        <property type="entry name" value="CfbD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000510">
        <property type="entry name" value="Nase/OxRdtase_comp1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000318">
        <property type="entry name" value="Nase_comp1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052673">
        <property type="entry name" value="Ni-siroh_cyclase_CfbD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03282">
        <property type="entry name" value="methan_mark_13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42846">
        <property type="entry name" value="NI-SIROHYDROCHLORIN A,C-DIAMIDE REDUCTIVE CYCLASE COMPLEX, COMPONENT CFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42846:SF1">
        <property type="entry name" value="NI-SIROHYDROCHLORIN A,C-DIAMIDE REDUCTIVE CYCLASE COMPLEX, COMPONENT CFBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00148">
        <property type="entry name" value="Oxidored_nitro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00699">
        <property type="entry name" value="NITROGENASE_1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitrogenase/oxidoreductase component 1" evidence="3">
        <location>
            <begin position="22"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU004021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="340" mass="38075" checksum="5922A0279BF4491E" modified="2013-06-26" version="1">MHPRPSPIAAAMYQLRDLGVDAIILHGPAGCCFRTARLLEMDGVRVFTTSMDENSIIFGAEDKLVETLDYVINYLNKEKPIIGVVGTCMSMIIGENLNVKDDRAIIIPVEVHSGLGDNTIGAIKAMEAALEVGLIDREEFERQCYLLKKATELEKKRGMAKEKYIKPSYEDDTNKVIEVLKDLKGEKIACVLNAKKETAYLFVHPLIVLNKYFDCINIANLDCSKGLKKIREDAKNILKRFKPHYITGSLDEYPIAGEKAVEILESLDISAVVVSGIPHALPIEKLDIIKIAISDGPRTYYPIKEIYDYAIIEFDAHAKVLGKRDVVKSRFGEILDYALR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRY1</accession>
    <name>N6VRY1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metal-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01283</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589920.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRY1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00033"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.251"/>
    <dbReference type="OrthoDB" id="59661at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014997">
        <property type="entry name" value="DUF1847"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08901">
        <property type="entry name" value="DUF1847"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="195" mass="22660" checksum="6492E398CB2F9E24" modified="2013-06-26" version="1">MRCARCNKKECKNGKNCKEEILDNILEEYKKEENLKIAKVSSFIEGNFYMKKTRLEEIIEFCKMMNYKKIGIAFCIGLEREAKILDEILSKHFEVYSVCCKVCGIDKDKFELTHINKEQKETMCNPIAQALILNEKKTDLNIIVGLCIGHDILFQKYSKAPTTTFVVKDRILAHNPLGAIYSKYYLNKFGVYDKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS24</accession>
    <name>N6VS24_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00876</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS24"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="135" mass="15606" checksum="14BAA71B78ED01A3" modified="2013-06-26" version="1" fragment="single">MDEKFASKFEIKILNELTNKTFDDLAIILKKIGGLDYRKKVYIGNICLGILEFDLKELKWKFQPYAGYYLIEKPKIKLKNTKKRIKGKKISTDLIENLDEFKSLQDGYVGVEIGNYVGVGIKKGDQLKIKDLIQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSF2</accession>
    <name>N6VSF2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">MscS Mechanosensitive ion channel</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_00110</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589711.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSF2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01146"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.22"/>
    <dbReference type="OrthoDB" id="11475at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049142">
        <property type="entry name" value="MS_channel_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011066">
        <property type="entry name" value="MscS_channel_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30566:SF5">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30566">
        <property type="entry name" value="YNAI-RELATED MECHANOSENSITIVE ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21088">
        <property type="entry name" value="MS_channel_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82689">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="77"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel transmembrane helices 2/3" evidence="9">
        <location>
            <begin position="78"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="8">
        <location>
            <begin position="120"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF21088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="309" mass="35858" checksum="1884578E01956CB3" modified="2013-06-26" version="1">MKLGLFIKIVILLFLITLLMYLLNLYTYISIILSYKEQIIIIGIIIISAMILIDVCSELIDKYSKNRDIGEVLTLKILVKYFIYLITFLLIIAVFYKNTPSLFVSLGLIGAAITYALQRPILNLAGWMIIVFTRTIKIGDRVAIKDVGIGDVFNIDMQHIYIRELDNLLDPTGRVLIVPNSYIFDRTIVNFTKGTSYIWDRLEVHFTYDSNLKKIEEIVFSSAYDVVGKTMEEQYKKWIKNRYILTGFLKDKPIIRIKLTRSSIAVRVLYLVDTFEKASVKTEIYKRIIERVKKEEDVSLAYPHIKVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSF9</accession>
    <name>N6VSF9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">A-type ATP synthase subunit E</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">atpE</name>
        <name evidence="9" type="ORF">J422_04408</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the V-ATPase E subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96081.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591794.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSF9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00642"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.864"/>
    <dbReference type="OrthoDB" id="4691at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06503">
        <property type="entry name" value="ATP-synt_Fo_b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.30">
        <property type="entry name" value="ATP synthase, E subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.620">
        <property type="entry name" value="F1F0 ATP synthase subunit B, membrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00311">
        <property type="entry name" value="ATP_synth_E_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028987">
        <property type="entry name" value="ATP_synth_B-like_membr_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038495">
        <property type="entry name" value="ATPase_E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002842">
        <property type="entry name" value="ATPase_V1_Esu"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45715">
        <property type="entry name" value="ATPASE H+-TRANSPORTING V1 SUBUNIT E1A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01991">
        <property type="entry name" value="vATP-synt_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81573">
        <property type="entry name" value="F1F0 ATP synthase subunit B, membrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160527">
        <property type="entry name" value="V-type ATPase subunit E-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="7" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 7" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="4 7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="8"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00311"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96081.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="204" mass="23399" checksum="63FE03770328873B" modified="2013-06-26" version="1">MGIEKIKEKILEDAKNEVRKIIAEAEEERKKILEEAKKEAERRKEQILKKGEKEAEMIKNRIIAEAKLEAKKMILKTKEELIEKAIVKLREDLLKYPESNEYKEKLIKLIMDGAIALGGGEILVRLNERDDKLIDAQVLWNLEKTIEDMTGKVTILKKGKPVNIIGGCILESSDGFKVLDNSLEAIFDRNLPNIRSKVMEILFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSH1</accession>
    <name>N6VSH1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pantoate kinase</fullName>
            <shortName evidence="1">PoK</shortName>
            <ecNumber evidence="1">2.7.1.169</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_04518</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Phosphorylates (R)-pantoate to form (R)-4-phosphopantoate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(R)-pantoate + ATP = (R)-4-phosphopantoate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28246"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15980"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.169"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the GHMP kinase family. PoK subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.169" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96101.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591836.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSH1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00621"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.886"/>
    <dbReference type="OrthoDB" id="85822at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02223">
        <property type="entry name" value="Pantoate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012043">
        <property type="entry name" value="PoK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282:SF1">
        <property type="entry name" value="PANTOATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42282">
        <property type="entry name" value="PANTOATE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016896">
        <property type="entry name" value="GHMP_arc_MJ0969"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="2">
        <location>
            <begin position="59"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96101.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="257" mass="28856" checksum="19F814DB2656C394" modified="2013-06-26" version="1">MVPAHITGFFTIYKDRDPMKTGSKGAGITLDRGVKLEIEEGKGVYYNNERVNIDPINYILKNYKINYKINFISDFPLGSGLGMSGACSLSLSKLIFKDFNAVKLAHKAEVLSGTGLGDVIAQYVRGFVIRKSPGFPINVVKVIDKDYYVIVEILGKKETKDVINNREFINKINYFGERCLKLLLENPNIKNFVKLSYDFALNIGLMDDEIKELCEDLKFTVGASQSMIGKTLFCIAKKEHLKDAISILKNPIVCHIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSH4</accession>
    <name>N6VSH4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">CODH nickel-insertion accessory protein CooC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_04553</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96106.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591846.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSH4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00615"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.893"/>
    <dbReference type="OrthoDB" id="31168at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001573">
        <property type="entry name" value="Carbon monoxide dehydrogenase accessory protein CooC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014433">
        <property type="entry name" value="CooC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF6">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005647">
        <property type="entry name" value="CooC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="3">
        <location>
            <begin position="5"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96106.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="249" mass="27687" checksum="5DDB65B3EB3E9F4D" modified="2013-06-26" version="1">MKIAIVGKGGVGKTFIASNLIKLFAKNGYKVIAIDCDNNPTLALSFNIYEDITPISKREDIIEERTGAKPGSFGAIFKINPKVDDLIDKLGYKVDDNITLLVMGTIEKSGEGCVCPASILLRRLLRHLVLKRDEVVILDMEAGLEHFGRKTLDGIDLMLIVVEPTKKSIITAKRIKKLAEDMGIKNIKVIINKVKGDIEKVKELVERELGIEVIGVIPYDEKVEKAELFGEELENSIALKEIEKIFKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT54</accession>
    <name>N6VT54_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Carbamoyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_02839</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NodU/CmcH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96376.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT54"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00117"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.557"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd24100">
        <property type="entry name" value="ASKHA_NBD_MJ1051-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.20">
        <property type="entry name" value="Carbamoyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031730">
        <property type="entry name" value="Carbam_trans_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038152">
        <property type="entry name" value="Carbam_trans_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003696">
        <property type="entry name" value="Carbtransf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051338">
        <property type="entry name" value="NodU/CmcH_Carbamoyltrnsfr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34847">
        <property type="entry name" value="NODULATION PROTEIN U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34847:SF1">
        <property type="entry name" value="NODULATION PROTEIN U"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16861">
        <property type="entry name" value="Carbam_trans_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02543">
        <property type="entry name" value="Carbam_trans_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbamoyltransferase" evidence="2">
        <location>
            <begin position="62"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="Carbamoyltransferase C-terminal" evidence="3">
        <location>
            <begin position="326"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006129"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96376.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="492" mass="56848" checksum="33B91CF0035CA2BB" modified="2013-06-26" version="1">MGIHDGHNAGAALIDKEIHANSEERFVRVKNYRGFPYKSIEYILRNKDFDYVTVAGVFRKQKRLKELRNFQEKVNKRFIYIYHHFAHSYLYKLSNFKEALVISIDGAGDGLSFLASIANKDLEIIAQSTYLDSVGDFYASITELLGFKPMEDEGKVMCLSAFDAEDNIDLKIIDYLENIRSFKNYLNCVGSEATKELKKILKFYNFKDMVRIAKYAQGELERVVLKAIDHLSNEYGVENIVFTGGVAQNVKLNSKIAEKYNLFVPPFMGDEGLCLGACLSDKRINRLEINNTYFGYEIKNNNVEPLLKNMKYEYIEDKDIPEVIGELIIDNKVVCIARGKMEFGPRALGNRSIIALPTKENKERINKNLKRNWFMPFAPTILYEYVEDYLINPTYSPFMTLLFKVKDDKIKEIEGVVHVDKTTRPQMLKRESNRIYYDIIKYIYDSINLPVVLNTSFNLHGEPIVCTEKDAINSFSRTDFDALLLGNYLIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT98</accession>
    <name>N6VT98_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0201 protein J422_02569</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02569</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0201 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96421.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT98"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00759"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.503"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01112">
        <property type="entry name" value="UPF0201"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="PAB1135-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652">
        <property type="entry name" value="UPF0201 PROTEIN TK1335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39652:SF1">
        <property type="entry name" value="UPF0201 PROTEIN TK1335"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96421.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="146" mass="16908" checksum="BF3E786946455FF3" modified="2013-06-26" version="1">MVIIKVIIKSKVKPTEDKYKVKKAILNIFPKANLRYSEKDFYGKWEGETKDVERFKELIRSQAILDTARMILEEKMDENSTRFYLNKQAAFVKAVNFDIDVHGGIYVKIIAEKGEDILKIIKDIAPRTKGGVIIEEEEEEIIEEKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTL2</accession>
    <name>N6VTL2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">O-phospho-L-seryl-tRNA:Cys-tRNA synthase</fullName>
            <ecNumber evidence="5">2.5.1.73</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Sep-tRNA:Cys-tRNA synthase</fullName>
            <shortName evidence="5">SepCysS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_01800</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Converts O-phospho-L-seryl-tRNA(Cys) (Sep-tRNA(Cys)) to L-cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>O-phospho-L-seryl-tRNA(Cys) + hydrogen sulfide + H(+) = L-cysteinyl-tRNA(Cys) + phosphate</text>
            <dbReference type="Rhea" id="RHEA:25686"/>
            <dbReference type="Rhea" id="RHEA-COMP:9679"/>
            <dbReference type="Rhea" id="RHEA-COMP:9719"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78517"/>
            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="EC" id="2.5.1.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer. Interacts with SepRS.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SepCysS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.73" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96511.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590117.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTL2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00210"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.354"/>
    <dbReference type="OrthoDB" id="5817at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043766">
        <property type="term" value="F:Sep-tRNA:Cys-tRNA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01675">
        <property type="entry name" value="Sep_Cys_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013375">
        <property type="entry name" value="Sep_Cys-tRNA_synth_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008829">
        <property type="entry name" value="SepSecS/SepCysS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006810">
        <property type="entry name" value="PRK09331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02539">
        <property type="entry name" value="SepCysS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586">
        <property type="entry name" value="CYSTEINE DESULFURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43586:SF3">
        <property type="entry name" value="O-PHOSPHO-L-SERYL-TRNA:CYS-TRNA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05889">
        <property type="entry name" value="SepSecS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="3 5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="83"/>
            <end position="84"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="213"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96511.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="375" mass="43088" checksum="23CFCA1F58A8DFF1" modified="2013-06-26" version="1">MNLDRYKNLKRQNRETINLNPIQRGGVIPSEAKKAIYEFWDGYSICDFCHGRLDEIDNPPVRDFLNDLSEFLNMDIVRLTHGAREGKFIVMHALCKEGDYIVLDKNAHYTSYLAAERAKLNIAEVGYEKEYPIYKIDLERYKEVIDELEDKNKSIGLILLTHVDGNFGNLNDAKKVGKIAKSKGYPFLLNCAYTVGRMPVDGKKLKADFIVASGHKSMAASGPIGVLAFNEEFSEVIKTSKRYPIKEVEFLGCTSRGLPLITLMASFPYVVNRVKYWEDEIKKTRYVVDELEKIGFKQLGIKPKEHDLIKFETPILDEIAKRDKRKGYFFYDELKKRGITGIKPGVTKEVKMSVYGLEWEQVEYVVNAIKEIVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTN5</accession>
    <name>N6VTN5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2341 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01920</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96531.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590142.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTN5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00232"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.378"/>
    <dbReference type="OrthoDB" id="66028at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018765">
        <property type="entry name" value="DUF2341"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10102">
        <property type="entry name" value="DUF2341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2341" evidence="2">
        <location>
            <begin position="214"/>
            <end position="296"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96531.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="525" mass="61552" checksum="171F093CE8AC1F1C" modified="2013-06-26" version="1">MYYTQIALVLVLLVFTITSVFYNTIDLKTSEIKNEIEVKMISLAEKNIEHTINHNLDKIVNDVFINVSYTLMKEHRFFNNSSSAEECIENNITYILNKTLYNVCRDNFTIIVSHIRINPTSEPTRILLTGEVLLKYRKELENNVSIIINKEIGIIKEITLKEIPDPYVYNNKFYYNWSYCSPVDVNVSNGHHIFKIILNNTNFNYTLMKNPNDPSEIRIIGSSKIANEYILLPYWIEKWRYNNISVIWVNCSEDNLINGKIFILYNSSTKINRENPHKTFILFDNFNYLDNNSWEITGGCWINNGLLYVKGPYSKLSTKRSFSYNYELIFRANFSSVMKLDSENISEFIGFFKNDSNGIGFIYYNTSWGKEGLYVRYGQNLSKIPNFSKYLNNFYIYSVSWGEDRVSFRIYNEYYNLLYNKSINININENYPISICTEGVLNATVLVDWLVLKDVSNIIAIPQKPMRNILDYHEEKPKTYKGTIYYGDPEQYIKVNDGRYSIIGMFTNATYKWGSCGYKPKIEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTW1</accession>
    <name>N6VTW1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable ribosomal RNA small subunit methyltransferase A</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">16S rRNA dimethyladenosine transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">16S rRNA dimethylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">S-adenosylmethionine-6-N',N'-adenosyl(rRNA) dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rsmA</name>
        <name evidence="7" type="synonym">ksgA</name>
        <name evidence="10" type="ORF">J422_01333</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Specifically dimethylates two adjacent adenosines in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7 8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589930.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTW1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00022"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.261"/>
    <dbReference type="OrthoDB" id="9883at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000179">
        <property type="term" value="F:rRNA (adenine-N6,N6-)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000023">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.100">
        <property type="entry name" value="Ribosomal RNA adenine dimethylase-like, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00607">
        <property type="entry name" value="16SrRNA_methyltr_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001737">
        <property type="entry name" value="KsgA/Erm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023165">
        <property type="entry name" value="rRNA_Ade_diMease-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020596">
        <property type="entry name" value="rRNA_Ade_Mease_Trfase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020598">
        <property type="entry name" value="rRNA_Ade_methylase_Trfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011530">
        <property type="entry name" value="rRNA_adenine_dimethylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00755">
        <property type="entry name" value="ksgA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11727:SF7">
        <property type="entry name" value="DIMETHYLADENOSINE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00398">
        <property type="entry name" value="RrnaAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00650">
        <property type="entry name" value="rADc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01131">
        <property type="entry name" value="RRNA_A_DIMETH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51689">
        <property type="entry name" value="SAM_RNA_A_N6_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 7 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="5 7 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribosomal RNA adenine methylase transferase N-terminal" evidence="9">
        <location>
            <begin position="18"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7 8">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00607"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00650"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="263" mass="30413" checksum="1E9B81AB7E2AC9B1" modified="2013-06-26" version="1">MFRYKKRLGQCFLKDKRYVIKAVEEAKLSKDDRVLEIGLGKGVLTEELCKRAGEVYVIEIDKSLSPYAEKLKEVYDNLNIIWEDALKVDLENIDFNKVVANLPYQISSPITFKLLKVGFDLAVLMYQWEFAKRMVAKEGEKDYGRLTVAVKVRAEPYIISKIPPSAFYPKPKVYSALVKLIPHNKYEIDEFFDTFLRAIFQHRNKTVRSSLISSSKEFGMDKKAMTKLLEGVNESILNKKVFQLNIDEIIKLSNIMKSLLKPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTW5</accession>
    <name>N6VTW5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Cation transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_01358</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TrkH potassium transport family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTW5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00017"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.266"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030001">
        <property type="term" value="P:metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003445">
        <property type="entry name" value="Cat_transpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32024">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32024:SF2">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02386">
        <property type="entry name" value="TrkH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="70"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="176"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="236"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="269"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="316"/>
            <end position="338"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="382"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="442"/>
            <end position="462"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="467" mass="51604" checksum="620A6CF8E5501083" modified="2013-06-26" version="1">MIMKKEDIYGILHILGGVIGIIGAFMLIPSVIAFYFKEPTILNFLIPSFIFIILGIGLNKKFKAKNLKLYQTMIASALSWLIASLIGAIPLYLSIPYFSYVDAVYESMSAWTTTGMTLIADVESLPNSVLFWRSFEQWIGGVGILVLSALILAGKGSVASLFYYSEARQERIMPSSLNTIKVIFSIYLFYTILGILALYLSGLSFWAALNLTMTGICTGGMSIYNYSFPNNIFAKIVMIIIMLIGGVVSFLIHHKILTGKCFKDIQTKYALIVIIISSLIIYFIDKIDIIDAIFITTSALTSTGFSTIDLTSLSNLSLFILIFIMLIGGGAGTTTGGVKIIRFLVVLKAIYYEIHSVIIPKSAVVYEHLEDIPLSYRIIREAFVIFSLYIITSFIIAMVFIALGYDPYKSIFDAVSFVSNIGLSLGVVSLKTPLIGKIVAIIGMWMGRLEFIPVLVLFAALLKKRLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU35</accession>
    <name>N6VU35_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hydroxymethylbilane synthase</fullName>
            <ecNumber evidence="8">2.5.1.61</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">hemC</name>
        <name evidence="11" type="ORF">J422_00606</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>4 porphobilinogen + H2O = hydroxymethylbilane + 4 NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:13185"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57845"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="EC" id="2.5.1.61"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>dipyrromethane</name>
            <dbReference type="ChEBI" id="CHEBI:60342"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the HMBS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.61" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589801.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU35"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00912"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.116"/>
    <dbReference type="OrthoDB" id="8042at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004418">
        <property type="term" value="F:hydroxymethylbilane synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13647">
        <property type="entry name" value="PBP2_PBGD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000005">
        <property type="entry name" value="Porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000086">
        <property type="entry name" value="Probable porphobilinogen deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.40">
        <property type="entry name" value="Porphobilinogen deaminase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000860">
        <property type="entry name" value="HemC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022417">
        <property type="entry name" value="Porphobilin_deaminase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022418">
        <property type="entry name" value="Porphobilinogen_deaminase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036803">
        <property type="entry name" value="Porphobilinogen_deaminase_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00212">
        <property type="entry name" value="hemC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11557:SF0">
        <property type="entry name" value="PORPHOBILINOGEN DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01379">
        <property type="entry name" value="Porphobil_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03900">
        <property type="entry name" value="Porphobil_deamC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001438">
        <property type="entry name" value="4pyrrol_synth_OHMeBilane_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00151">
        <property type="entry name" value="PORPHBDMNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54782">
        <property type="entry name" value="Porphobilinogen deaminase (hydroxymethylbilane synthase), C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Porphobilinogen deaminase N-terminal" evidence="9">
        <location>
            <begin position="2"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="domain" description="Porphobilinogen deaminase C-terminal" evidence="10">
        <location>
            <begin position="212"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004735"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR00212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="281" mass="31768" checksum="C1E67DD593D0EC85" modified="2013-06-26" version="1">MIVGTRGSKLALCQTEKVVNELKKLGYDVNIKIIKTTGDKILDKKLSDIGIGVFTKELDLALLNNEIDIAVHSYKDIPTVWHDELTVAAVLKRDSPYDLMIWRKDVKDIKVIGTSSQRRRAFLKELYPNAEFKLLRGNVDTRLRKLRDKMYDAIVLAEAGIERLKIDLSEFNYKRLNILPAPAQGIIAVACRKGDREIINILKKINHEESYLLAECERAALRAFGGGCLEPFGALAEYKDNILYLKAKTIKKEALGRVRCKIDDIKMARQLGEDIGRILAS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU50</accession>
    <name>N6VU50_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MnmC-like methyltransferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00736</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96721.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589821.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU50"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00889"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.142"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016645">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-NH group of donors"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008471">
        <property type="entry name" value="MnmC-like_methylTransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39963:SF1">
        <property type="entry name" value="MNMC-LIKE METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39963">
        <property type="entry name" value="SLL0983 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05430">
        <property type="entry name" value="Methyltransf_30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MnmC-like methyltransferase" evidence="1">
        <location>
            <begin position="147"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96721.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="355" mass="41847" checksum="7103EF208FEC4DE0" modified="2013-06-26" version="1">MLVNSKALSIIRKYIKKYNLENEDYIKGKIINELKEKNLLVETEDGSLTLVSEDINEMMHSRIGALTEAIYKFVKPSNLEKMKEPKILDLCSGLCYNAVAALHFNKRAKIDMVEISEEVLFLSLFLDIPFEEHEIIKDKIREHFLPNYKSEHENIKLYIEDARKFIKKSQSCYDIVFHDAFSPKRDPTLYTYDFLREVYLRLKNGGVLLSYSSSIPFRSALVDNSFIISEEESLDRKRCISLAYKNRWSKRINEVDERVIALSITALPYRDKKLNARKEEIINEREKNKMILKDILTKKGKYISTKKIKKGKIPKELLDIQKENLNSTEIIKKMRKTYYGYYSFQDLIENFSHLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU74</accession>
    <name>N6VU74_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0248 protein J422_00476</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00476</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0248 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU74"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00047"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.91"/>
    <dbReference type="OrthoDB" id="14794at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01245">
        <property type="entry name" value="UPF0248"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040459">
        <property type="entry name" value="MJ1316"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007547">
        <property type="entry name" value="UPF0248"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003272">
        <property type="entry name" value="PRK04257.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04457">
        <property type="entry name" value="MJ1316"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MJ1316 RNA cyclic group end recognition" evidence="2">
        <location>
            <begin position="3"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="74" mass="9085" checksum="C9BC0E01CEA743D6" modified="2013-06-26" version="1">MLKDIINKILWHPKYKREDYYLIILHRGEKDNKKIIPMDKIKLEGGYIIYNDTYIPLHRVLEIRKKNGELIYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VWV0</accession>
    <name>N6VWV0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AIG2 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_07197</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000078">
        <property type="protein sequence ID" value="ENN95562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VWV0"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1390"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-glutamylcyclotransferase AIG2-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="45" mass="5405" checksum="A0929C5DF23E8E0A" modified="2013-06-26" version="1">MQYIFVYGTLRRGFWNNKLLKNSKFIGKGRTKEKYAMYADIIPML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX25</accession>
    <name>N6VX25_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Archaeoflavoprotein AfpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06618</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000056">
        <property type="protein sequence ID" value="ENN95662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX25"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00363"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1288"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014072">
        <property type="entry name" value="Archaeoflavo_AfpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02699">
        <property type="entry name" value="archaeo_AfpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF19">
        <property type="entry name" value="FLAVOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="1">
        <location>
            <begin position="1"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="130" mass="14531" checksum="FFFE449F7788FC7C" modified="2013-06-26" version="1">MKIAWGITGCGDKIEEVVNLMIKLKNKYNLDIDVYTSKSGKVVLKWYKLWDLLKDEFYDVRVEVDANAPFLPGKLQTGKYDLFIVAPATANTVAKIAHCIADTLITNSVAQAVKAKVPVYIFPPDNRVGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX98</accession>
    <name>N6VX98_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">6-pyruvoyl tetrahydrobiopterin synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_06060</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593225.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX98"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00531"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1180"/>
    <dbReference type="OrthoDB" id="6529at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.10">
        <property type="entry name" value="6-pyruvoyl tetrahydropterin synthase/QueD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007115">
        <property type="entry name" value="6-PTP_synth/QueD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038418">
        <property type="entry name" value="6-PTP_synth/QueD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589:SF7">
        <property type="entry name" value="6-PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12589">
        <property type="entry name" value="PYRUVOYL TETRAHYDROBIOPTERIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01242">
        <property type="entry name" value="PTPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55620">
        <property type="entry name" value="Tetrahydrobiopterin biosynthesis enzymes-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="163" mass="19093" checksum="71D1040D7F9D6EDB" modified="2013-06-26" version="1">MIIEIVGLYANLKFSSAHIVFGHRTCGVIHGHTYYVDIKVCGDKGEFNFVCDFKIIKEIVKEICKKIDHKLILPRDHKDVYYELRDKALYFKYKNKEYIIPVEDVILLPIETTSAEDLAKFFAEEIISRLKEKYKNVYWIEVAISEGLGQKAIYKLEVKHCKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXB3</accession>
    <name>N6VXB3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2111 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06170</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593267.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXB3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00553"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1202"/>
    <dbReference type="OrthoDB" id="3369at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012029">
        <property type="entry name" value="UCP006557"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09884">
        <property type="entry name" value="DUF2111"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006557">
        <property type="entry name" value="UCP006557_sign"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="127" mass="14007" checksum="46A181715E450F7A" modified="2013-06-26" version="1">MIISENSKAEDLIPIAKAIHILVNKLPITMRSKNYPGVRLEKGEVVDKNFEGYVLKLAIEKGEVIKATPIIGPYKGLPVIVVPIKENDKVVGAVGVVDITAGIFEDILAIARRPELYKFLPEDAYPR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXJ5</accession>
    <name>N6VXJ5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Malate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_05529</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LDH2/MDH2 oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000042">
        <property type="protein sequence ID" value="ENN95857.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592609.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXJ5"/>
    <dbReference type="SMR" id="N6VXJ5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00746"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1079"/>
    <dbReference type="OrthoDB" id="40552at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1530.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.60">
        <property type="entry name" value="Hypothetical oxidoreductase yiak, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053453">
        <property type="entry name" value="LDH2/MDH2_Oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043144">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_ah"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043143">
        <property type="entry name" value="Mal/L-sulf/L-lact_DH-like_NADP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036111">
        <property type="entry name" value="Mal/L-sulfo/L-lacto_DH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003767">
        <property type="entry name" value="Malate/L-lactate_DH-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040650">
        <property type="entry name" value="sulfolac_dhydr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11091:SF0">
        <property type="entry name" value="MALATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11091">
        <property type="entry name" value="OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02615">
        <property type="entry name" value="Ldh_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89733">
        <property type="entry name" value="L-sulfolactate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95857.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="344" mass="37602" checksum="968B314A1081F14C" modified="2013-06-26" version="1">MLIKPEKAKKLIIDVLKRYDVPKEDAEIVSDVLIEADLKGFSSHGIGRFPQYIKGLKLKNINPKPNIKIIKDKKATAVIDGDLGFGQVVAKKAMDLAIDKAKDYGISAVATVNSNHFGIAGYYAEMAMKNDMVGIVITNTEPAMAPYGGTEKILGTNPIAIAFRGNKYKFSLDMATASIARGKIFEALRKGEKIPFGCAVDKDGNITDDPKKALEGSILPFGGAKGYGLALAIEMLSAIGGAEVGKGVKGTANPEERCTKGDLFVVINPDFFMGKEEFKKKVDKLMEEIKTSKPMKDHEILIPGEIEERNKIKNKEGIEIDEVLYAQLKEILKEVGLNVEDYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXR8</accession>
    <name>N6VXR8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8 13">Glutamyl-tRNA reductase</fullName>
            <shortName evidence="8">GluTR</shortName>
            <ecNumber evidence="3 8 13">1.2.1.70</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8 17" type="primary">hemA</name>
        <name evidence="17" type="ORF">J422_04825</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the NADPH-dependent reduction of glutamyl-tRNA(Glu) to glutamate 1-semialdehyde (GSA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8 13">
            <text>(S)-4-amino-5-oxopentanoate + tRNA(Glu) + NADP(+) = L-glutamyl-tRNA(Glu) + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12344"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57501"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="EC" id="1.2.1.70"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8 13">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; 5-aminolevulinate from L-glutamyl-tRNA(Glu): step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">Possesses an unusual extended V-shaped dimeric structure with each monomer consisting of three distinct domains arranged along a curved 'spinal' alpha-helix. The N-terminal catalytic domain specifically recognizes the glutamate moiety of the substrate. The second domain is the NADPH-binding domain, and the third C-terminal domain is responsible for dimerization.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">During catalysis, the active site Cys acts as a nucleophile attacking the alpha-carbonyl group of tRNA-bound glutamate with the formation of a thioester intermediate between enzyme and glutamate, and the concomitant release of tRNA(Glu). The thioester intermediate is finally reduced by direct hydride transfer from NADPH, to form the product GSA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 13">Belongs to the glutamyl-tRNA reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.70" evidence="3 8 13"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95937.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXR8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00525"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.945"/>
    <dbReference type="OrthoDB" id="4562at2157"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00316"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008883">
        <property type="term" value="F:glutamyl-tRNA reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019353">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process from glutamate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05213">
        <property type="entry name" value="NAD_bind_Glutamyl_tRNA_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000031">
        <property type="entry name" value="Glutamyl-tRNA reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.70">
        <property type="entry name" value="Glutamyl tRNA-reductase dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.30">
        <property type="entry name" value="Glutamyl-tRNA reductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00087">
        <property type="entry name" value="Glu_tRNA_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000343">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015896">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015895">
        <property type="entry name" value="4pyrrol_synth_GluRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018214">
        <property type="entry name" value="GluRdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036453">
        <property type="entry name" value="GluRdtase_dimer_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036343">
        <property type="entry name" value="GluRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01035">
        <property type="entry name" value="hemA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43013">
        <property type="entry name" value="GLUTAMYL-TRNA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43013:SF1">
        <property type="entry name" value="GLUTAMYL-TRNA REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00745">
        <property type="entry name" value="GlutR_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05201">
        <property type="entry name" value="GlutR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000445">
        <property type="entry name" value="4pyrrol_synth_GluRdtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69742">
        <property type="entry name" value="Glutamyl tRNA-reductase catalytic, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69075">
        <property type="entry name" value="Glutamyl tRNA-reductase dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00747">
        <property type="entry name" value="GLUTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 11" id="KW-0521">NADP</keyword>
    <keyword evidence="5 8 13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 8 13" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl-tRNA reductase N-terminal" evidence="16">
        <location>
            <begin position="7"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="domain" description="Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase" evidence="15">
        <location>
            <begin position="147"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrapyrrole biosynthesis glutamyl-tRNA reductase dimerisation" evidence="14">
        <location>
            <begin position="285"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8 9">
        <location>
            <position position="40"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <begin position="39"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <begin position="90"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 10">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8 11">
        <location>
            <begin position="160"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for activity" evidence="8 12">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000445-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF05201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN95937.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="361" mass="41812" checksum="5852E1ABE25287BE" modified="2013-06-26" version="1">MIIVLKADYKKYSVNDLEKLRFNEKEFYSKYENAILLQTCNRVELIFYGYSLDEIKNIKNFEKFDLLLEDEAILHLFRLAAGLESMIVGEVQILGQIKDAFLKANIKDKRFEKIMLKIIHTGQRVREETEISKGSVSIGSAAVELAEKFGLEGKNILLIGAGEMATLVIKALKEKNVKAIIIANRTYEKALKLAKELGGIAVRFDKLKEALKYADIVISATSAPHPILTKERVMDIEETIIIDIANPRDTTDDIRELKHIKLFTIDDLKIIAEENLKRRMKEIPKVEKIIKEEFENLKKLLKELEIEEKIKDFCINLENLRIREVEKAKKLLKTRDPERVLEDFSRSFCKKIIYDLKRVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6VY38</accession>
    <name>N6VY38_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04610</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000029">
        <property type="protein sequence ID" value="ENN96037.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591920.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VY38"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.903"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96037.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="41" mass="4530" checksum="B1749ECD3F4EBA03" modified="2013-06-26" version="1">MDNQTLFMAMGLASLGLVVLLSLALYVWYFKLGGKKALDTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>N6VY86</accession>
    <name>N6VY86_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable tRNA sulfurtransferase</fullName>
            <ecNumber evidence="9">2.8.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Sulfur carrier protein ThiS sulfurtransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Thiamine biosynthesis protein ThiI</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">tRNA 4-thiouridine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">thiI</name>
        <name evidence="12" type="ORF">J422_04328</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. This is a step in the synthesis of thiazole, in the thiamine biosynthesis pathway. The sulfur is donated as persulfide by IscS.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[ThiI sulfur-carrier protein]-S-sulfanyl-L-cysteine + a uridine in tRNA + 2 reduced [2Fe-2S]-[ferredoxin] + ATP + H(+) = [ThiI sulfur-carrier protein]-L-cysteine + a 4-thiouridine in tRNA + 2 oxidized [2Fe-2S]-[ferredoxin] + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:24176"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:13337"/>
            <dbReference type="Rhea" id="RHEA-COMP:13338"/>
            <dbReference type="Rhea" id="RHEA-COMP:13339"/>
            <dbReference type="Rhea" id="RHEA-COMP:13340"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:136798"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.8.1.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>[ThiS sulfur-carrier protein]-C-terminal Gly-Gly-AMP + S-sulfanyl-L-cysteinyl-[cysteine desulfurase] + AH2 = [ThiS sulfur-carrier protein]-C-terminal-Gly-aminoethanethioate + L-cysteinyl-[cysteine desulfurase] + A + AMP + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43340"/>
            <dbReference type="Rhea" id="RHEA-COMP:12157"/>
            <dbReference type="Rhea" id="RHEA-COMP:12158"/>
            <dbReference type="Rhea" id="RHEA-COMP:12910"/>
            <dbReference type="Rhea" id="RHEA-COMP:19908"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29950"/>
            <dbReference type="ChEBI" id="CHEBI:61963"/>
            <dbReference type="ChEBI" id="CHEBI:90618"/>
            <dbReference type="ChEBI" id="CHEBI:232372"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ThiI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.4" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96067.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591764.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VY86"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00658"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.848"/>
    <dbReference type="OrthoDB" id="372227at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140741">
        <property type="term" value="F:tRNA-uracil-4 sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002937">
        <property type="term" value="P:tRNA 4-thiouridine biosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01712">
        <property type="entry name" value="PPase_ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11716">
        <property type="entry name" value="THUMP_ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000053">
        <property type="entry name" value="Probable tRNA sulfurtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00021">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020536">
        <property type="entry name" value="ThiI_AANH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054173">
        <property type="entry name" value="ThiI_fer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049961">
        <property type="entry name" value="ThiI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049962">
        <property type="entry name" value="THUMP_ThiI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003720">
        <property type="entry name" value="tRNA_STrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050102">
        <property type="entry name" value="tRNA_sulfurtransferase_ThiI"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00342">
        <property type="entry name" value="tRNA uracil 4-sulfurtransferase ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209:SF1">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02568">
        <property type="entry name" value="ThiI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22025">
        <property type="entry name" value="ThiI_fer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="8 9" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="3 9" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="11">
        <location>
            <begin position="60"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="186"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96067.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="373" mass="42687" checksum="E4B8F28A59DCB113" modified="2013-06-26" version="1">MEILVRYGEIGLKSDIIRRNLEEILRKNIIKLLKKYDIDADVYILHRRLLVNVNTKDKEDEAIDLLRKVAGIVSYSPGYSCPLDIDEIINTAIQVMKKKMKDLGKEKIKFAVKTKRSNKQFPFNSVEVNKKVGEAIVEKFNLDVDLDNPDITLGIEILRDRAFIFTDKFEGIGGLPVGSQGRVLCLISDGIDSPVAAFLMAKRGCRLVLLHLKITEEALNKVRKIVEVLSDYDTELEFVVYDYKKDLEEIYKKLKEIKKESYTCIFCKKKMLKIAEKYAKYMECDAIVTGDCLGQVASQTLKNLRVISQGINYLILRPLIAFDKNETTELAKKIGTYDIAIEKEIKCPYLPKFPKTLSKIEEVEKIMKKANIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYE7</accession>
    <name>N6VYE7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Coenzyme F420 hydrogenase subunit beta</fullName>
            <ecNumber evidence="7">1.12.98.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_03748</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the FrhB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.1" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96142.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591334.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYE7"/>
    <dbReference type="SMR" id="N6VYE7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00382"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.732"/>
    <dbReference type="OrthoDB" id="37898at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052592">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017679">
        <property type="entry name" value="FrhB_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03289">
        <property type="entry name" value="frhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006807">
        <property type="entry name" value="PRK09325.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="7-HYDROXYMETHYL CHLOROPHYLL A REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332:SF6">
        <property type="entry name" value="FORMATE DEHYDROGENASE SUBUNIT BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme F420 hydrogenase/dehydrogenase beta subunit N-terminal" evidence="8">
        <location>
            <begin position="10"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme F420 hydrogenase/dehydrogenase beta subunit C-terminal" evidence="9">
        <location>
            <begin position="95"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="NCBIfam" id="TIGR03289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96142.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="282" mass="31532" checksum="4CB3D1BDF99980A0" modified="2013-06-26" version="1">MSELGKYIKVVSARSKLKEVLKKSQDGGIVSTAFIYGLENNLLDGVIVADKKDGFMPYPKVATTVEEVLEAAGTKYSVCPNISVLKSAAREYGCEKIGVVGTPCQVRAVRKLIKYPLGFRHVADKIALIIGIFCMENFPYEGLKLIVEEHCNVKMDDVVKLDIGKGKFWVYTRWGETKAIKLKETHPYEQTSCHVCLDYTAELADISTGSVGSPDGWSTVFIRTKKGEEFFNKMVEDGYLEIKPIEEVKPGLDLVIKLAKTKKDKNSKEIEHRKELNLPVPH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYU6</accession>
    <name>N6VYU6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13 16">Nucleoside diphosphate kinase</fullName>
            <shortName evidence="13">NDK</shortName>
            <shortName evidence="13">NDP kinase</shortName>
            <ecNumber evidence="3 13 16">2.7.4.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Nucleoside-2-P kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">ndk</name>
        <name evidence="18" type="ORF">J422_03044</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 16">
            <text>a 2'-deoxyribonucleoside 5'-diphosphate + ATP = a 2'-deoxyribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:44640"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:73316"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>a ribonucleoside 5'-diphosphate + ATP = a ribonucleoside 5'-triphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:18113"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57930"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 14 15">Belongs to the NDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.6" evidence="3 13 16"/>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96302.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590753.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYU6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00156"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.597"/>
    <dbReference type="OrthoDB" id="6874at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004550">
        <property type="term" value="F:nucleoside diphosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006241">
        <property type="term" value="P:CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006228">
        <property type="term" value="P:UTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04413">
        <property type="entry name" value="NDPk_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.141:FF:000003">
        <property type="entry name" value="Nucleoside diphosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.141">
        <property type="entry name" value="Nucleoside diphosphate kinase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00451">
        <property type="entry name" value="NDP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034907">
        <property type="entry name" value="NDK-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036850">
        <property type="entry name" value="NDK-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001564">
        <property type="entry name" value="Nucleoside_diP_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023005">
        <property type="entry name" value="Nucleoside_diP_kinase_AS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001908">
        <property type="entry name" value="PRK00668.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011112">
        <property type="entry name" value="PRK14540.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11349">
        <property type="entry name" value="NUCLEOSIDE DIPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00334">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01243">
        <property type="entry name" value="NUCDPKINASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00562">
        <property type="entry name" value="NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54919">
        <property type="entry name" value="Nucleoside diphosphate kinase, NDK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00469">
        <property type="entry name" value="NDPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51374">
        <property type="entry name" value="NDPK_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 13" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="8 13 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 13" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside diphosphate kinase-like" evidence="17">
        <location>
            <begin position="1"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="active site" description="Pros-phosphohistidine intermediate" evidence="13 14">
        <location>
            <position position="115"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU004013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="SMART" id="SM00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ENN96302.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="134" mass="15343" checksum="C509E9C2CC312DAF" modified="2013-06-26" version="1">MERTLVIIKPDAVRRKLIGRIIQRIEDKGLEIIAMKMLKLSREEAEEFYKEHIGKEFYESLVNFMSSGRIVAMVVEGENAISVIRKLIGKTDPYEADPGTIRGDFALKLPENVIHASDSKASAEREIKFFFKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYW6</accession>
    <name>N6VYW6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Nuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_03144</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96322.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590786.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYW6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00175"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.617"/>
    <dbReference type="OrthoDB" id="3327at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00175">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035437">
        <property type="entry name" value="SNase_OB-fold_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016071">
        <property type="entry name" value="Staphylococal_nuclease_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002071">
        <property type="entry name" value="Thermonucl_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302">
        <property type="entry name" value="EBNA2 BINDING PROTEIN P100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12302:SF3">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE 31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00565">
        <property type="entry name" value="SNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00318">
        <property type="entry name" value="SNc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50199">
        <property type="entry name" value="Staphylococcal nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01123">
        <property type="entry name" value="TNASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01284">
        <property type="entry name" value="TNASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50830">
        <property type="entry name" value="TNASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TNase-like" evidence="4">
        <location>
            <begin position="24"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96322.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="179" mass="21419" checksum="00514742BAD9697E" modified="2013-06-26" version="1">MRILTIFLILFLTLSGCITYNEYSCYYGRVVKVVDGDTVYVLVNGELWKIRLLGVDTPEISKENNPYEYILYNGTPITNLTYLKFWGERAKEFAYKKLYGKEVKIVFDPLAPRKDRYGRYLAYIYYKKGDKWVNFNEELIKYGYARVYRTRFKMLHEFLMLEEEAKKNRVGLWKGQLMP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ73</accession>
    <name>N6VZ73_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Protease synthase and sporulation negative regulator Pai1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_02340</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the acetyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96427.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590334.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ73"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00349"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.460"/>
    <dbReference type="OrthoDB" id="38613at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016747">
        <property type="term" value="F:acyltransferase activity, transferring groups other than amino-acyl groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.630.30:FF:000282">
        <property type="entry name" value="GCN5-related N-acetyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050680">
        <property type="entry name" value="YpeA/RimI_acetyltransf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011470">
        <property type="entry name" value="PRK14887.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43420">
        <property type="entry name" value="ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43420:SF47">
        <property type="entry name" value="N-ACETYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="4">
        <location>
            <begin position="72"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96427.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="225" mass="27062" checksum="D2E39F19966FBCF0" modified="2013-06-26" version="1">MWVIFDLNIDIPKEVSRSLEIDNYKENSFEITLKNNNLHIETHSIGSLKSILDDFFRCLTAAIKVYEIACDYKIRNVTESDLESFLSLYYKAYKGFKKYYYKTERWAKWYFKWLMKRDKDGFFIIEYKNKPIGFIACDCNWESKIEKKKVAEIHEIFVDPDFRGRGIGTMLINKAIEYAKKRGRDVIELWAGVENEQAKKFYKHLGFEEKEKVNDWVRFVKRLQF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZY8</accession>
    <name>N6VZY8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Seryl-tRNA ligase</fullName>
            <ecNumber evidence="1">6.1.1.11</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01156</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.11" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZY8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00813"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.226"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004828">
        <property type="term" value="F:serine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="82" mass="9678" checksum="B03135EC3C33D888" modified="2013-06-26" version="1">METKEEKGVAVVSANVHGTHFVEGFRIKDYKNRRVWTGCTGFGITRWVYGFLSQYGFNYDDWPDEIKKRVEKIETVKMITWP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6W0D6</accession>
    <name>N6W0D6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-oxoglutarate ferredoxin oxidoreductase subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00280</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589743.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W0D6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01107"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.56"/>
    <dbReference type="OrthoDB" id="30755at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045333">
        <property type="term" value="P:cellular respiration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03375">
        <property type="entry name" value="TPP_OGFOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051457">
        <property type="entry name" value="2-oxoacid:Fd_oxidoreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084">
        <property type="entry name" value="2-OXOGLUTARATE OXIDOREDUCTASE SUBUNIT KORB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48084:SF1">
        <property type="entry name" value="2-OXOGLUTARATE SYNTHASE SUBUNIT KORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="2">
        <location>
            <begin position="50"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="269" mass="29868" checksum="3EE3572A36BECCA7" modified="2013-06-26" version="1">MHFAIKYLREDRLPHIFCPGCGNGIVINCFVKALIDLNIKPEDYIAVSGIGCSSRIPGYLYCDSLHTTHGRAIAFATGVKIARPDKYVVVFTGDGDLAAIGGNHFIHGCRRNIDLTVICVNNNIYGMTGGQVSPTTPHGKRATTAPYGNIENELDLCKLAISAGASYVARWTTAHPIQLVRSIKKGLKKKGFAFIEVISQCPTYYGRLNISRKPVDVLKYIKDHSININKAKNMDEEELKDKIIVGEFLDVEKKEFVEELNKLIEKVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTG6</accession>
    <name>N6UTG6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TIGR00299 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06987</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000066">
        <property type="protein sequence ID" value="ENN95599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594667.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTG6"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1353"/>
    <dbReference type="OrthoDB" id="10691at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.300">
        <property type="entry name" value="mk0293 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1380">
        <property type="entry name" value="Transcriptional regulatory protein pf0864 domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002822">
        <property type="entry name" value="Ni_insertion"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36566">
        <property type="entry name" value="NICKEL INSERTION PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36566:SF1">
        <property type="entry name" value="PYRIDINIUM-3,5-BISTHIOCARBOXYLIC ACID MONONUCLEOTIDE NICKEL INSERTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01969">
        <property type="entry name" value="Ni_insertion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0533">Nickel</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="100" mass="11660" checksum="AD050403272673F0" modified="2013-06-26" version="1" fragment="single">KNRPGYLIRVIVDRDKAIETAKRLMKETGTLGVRIIDIKRIKANREVKEINYMGEKVKVKVSDIDGCIISKKPEYEDLKKIAKKYNKPLKDIYNEVKKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTJ4</accession>
    <name>N6UTJ4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Orotidine 5'-phosphate decarboxylase</fullName>
            <ecNumber evidence="2 9">4.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_06743</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>orotidine 5'-phosphate + H(+) = UMP + CO2</text>
            <dbReference type="Rhea" id="RHEA:11596"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="EC" id="4.1.1.23"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the OMP decarboxylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.23" evidence="2 9"/>
    <dbReference type="EMBL" id="APMM01000058">
        <property type="protein sequence ID" value="ENN95644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593991.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTJ4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01357"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1312"/>
    <dbReference type="OrthoDB" id="94124at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00120"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04725">
        <property type="entry name" value="OMP_decarboxylase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014732">
        <property type="entry name" value="OMPdecase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018089">
        <property type="entry name" value="OMPdecase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010386">
        <property type="entry name" value="PRK13813.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01740">
        <property type="entry name" value="pyrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32119:SF2">
        <property type="entry name" value="OROTIDINE 5'-PHOSPHATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00156">
        <property type="entry name" value="OMPDECASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="6 9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 9" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orotidine 5'-phosphate decarboxylase" evidence="10">
        <location>
            <begin position="2"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="7">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="7">
        <location>
            <position position="59"/>
        </location>
    </feature>
    <feature type="active site" description="For OMPdecase activity" evidence="7">
        <location>
            <position position="62"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR614732-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="210" mass="23316" checksum="FA33BEDC507D9CB7" modified="2013-06-26" version="1">MKLMLALDVEDKETAIRILDNVIDYIDAIKVGYPLVLPYGIKILKEIKRYNKEIICDFKLADIPATNEKIAKISLKYADGIIAHGFVGEDSLKAVIEIAKNMGKKTAVVVEMSHPGAVRYMQPIAEDLALLAKNLKADYIVAPSTRPKRLKKLKEISSLPVMTPGIITQGGKIEEIKDILDEKDYIIVGRAIYQANNPKESAKQIKDLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTK2</accession>
    <name>N6UTK2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Bifunctional formaldehyde-activating enzyme/3-hexulose-6-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_06688</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000057">
        <property type="protein sequence ID" value="ENN95654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593910.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTK2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00713"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1302"/>
    <dbReference type="OrthoDB" id="64276at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033982">
        <property type="term" value="F:3-dehydro-L-gulonate-6-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004590">
        <property type="term" value="F:orotidine-5'-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016051">
        <property type="term" value="P:carbohydrate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019854">
        <property type="term" value="P:L-ascorbic acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04726">
        <property type="entry name" value="KGPDC_HPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.60">
        <property type="entry name" value="Formaldehyde-activating enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014826">
        <property type="entry name" value="HCHO-activating_enzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037075">
        <property type="entry name" value="HCHO-activating_enzyme_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041710">
        <property type="entry name" value="HPS/KGPDC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001754">
        <property type="entry name" value="OMPdeCOase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009833">
        <property type="entry name" value="PRK13307.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35039">
        <property type="entry name" value="3-KETO-L-GULONATE-6-PHOSPHATE DECARBOXYLASE SGBH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35039:SF3">
        <property type="entry name" value="3-KETO-L-GULONATE-6-PHOSPHATE DECARBOXYLASE SGBH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08714">
        <property type="entry name" value="Fae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00215">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00934">
        <property type="entry name" value="OMPdecase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orotidine 5'-phosphate decarboxylase" evidence="3">
        <location>
            <begin position="154"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="372" mass="42031" checksum="54CB192331C477C1" modified="2013-06-26" version="1">MKFGEATFNNASVIASLVDDIVFSNALSRGNGILANLRPNLIVKPLTLVIPRGNLNNIQEELFNGVIQFAVAKAVADMDLDENLKILVTVNVPEEPLTMISKRKLFHQYYAATRLAINRAINEYPPMEKVKNEKFRAIHPLVGFRDDRLENPPYLQVALDVPSLENLDMILETLPKSDHLILEAGTPLIKRFGLEVIEHIREYFDGFIVADLKTLDTGRVEVRLAFEYTANAVVISALAPKATILKALHECQKCGLMSYLDTLNVDNIEMLYNSLEIKPDVIVLHRGIDEESFGIKKSWSFKEGKLAIAGGVGLENIDELIKNYHILIVGRAITKSKDPSRMIRAFLNKMGYDIDTYRLYYDEDEDLNYSFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTS7</accession>
    <name>N6UTS7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06095</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593240.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTS7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00538"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1187"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="64" mass="7506" checksum="2E2705D9447CC917" modified="2013-06-26" version="1">MDEIDIKAIQLINKAILSDEREIEATIKTLKNLAKIKKRKTKKAIRDILDYWASEAYKLDMEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTV5</accession>
    <name>N6UTV5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cysteine-rich small domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05953</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593039.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTV5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00568"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1159"/>
    <dbReference type="OrthoDB" id="39225at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007212">
        <property type="entry name" value="Zf-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04071">
        <property type="entry name" value="zf-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cysteine-rich small" evidence="1">
        <location>
            <begin position="14"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="116" mass="14088" checksum="0801D4D3FD2F87D6" modified="2013-06-26" version="1">MIKMAEDFFRKLFNIVINKSCPYYPCHFDGQTCIWCFCPFYPCEDQELGKYIERKDGSKVWSCENCWWIHREDVACEVLKEILKLVKDKDIDEMIEILKDHKLMLEIKEKVKERVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTX7</accession>
    <name>N6UTX7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DNA helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_05678</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DNA2/NAM7 helicase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592786.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTX7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01267"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1107"/>
    <dbReference type="OrthoDB" id="45637at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18808">
        <property type="entry name" value="SF1_C_Upf1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.270:FF:000007">
        <property type="entry name" value="DNA helicase, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000326">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050534">
        <property type="entry name" value="Coronavir_polyprotein_1ab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041679">
        <property type="entry name" value="DNA2/NAM7-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041677">
        <property type="entry name" value="DNA2/NAM7_AAA_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047187">
        <property type="entry name" value="SF1_C_Upf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004483">
        <property type="entry name" value="SMUBP-2/Hcs1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00376">
        <property type="entry name" value="IGHMBP2 family helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788:SF8">
        <property type="entry name" value="DNA-BINDING PROTEIN SMUBP-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43788">
        <property type="entry name" value="DNA2/NAM7 HELICASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13086">
        <property type="entry name" value="AAA_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13087">
        <property type="entry name" value="AAA_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="171"/>
            <end position="455"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00487"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="639" mass="74985" checksum="E6F9EA8335580E83" modified="2013-06-26" version="1">MNLIDHYVRKYLNLIELERKYEMEFHKEEIKRLGRKREEVGRAILNLKGTFLGEALGCTLVRFGRKKEFKTEISPGDVVLVSKDDPLKSDLYANVIYVGKNFIDVAFDIDVPKWVFKERVRIDLFINDITFKRMKEALLKIAKEKPKLLYIILGIEKPEKPFREDINIEFYDKSLNESQRYAVKMAVLNKDLYLIHGPPGTGKTRTLTEVIVQEVRFNKHKVLATADSNIAADNILEYLLKKYPDLNVVRVGHPTRINKELIEHSLPYLIETHEKYQEIKRIKEKIKKLKEERDKFLKPSPKWRRGLSDDKIVKIAKKNKDYRGIPKQKIKSMAEWIVRNRVIKRFVDKLDKIEKDIVDDILKKADVVVATNSMAGSEILKDYYFDVAVIDEASQAMEPSCLIPITKANKLIMAGDHKQLPPTVLSDNEELKKTLFEKLINKYPEFSTILEIQYRMNEKIMEFPNRMFYNNRLKAHESVKNITLLDLIKDVDEEDRDIINEKPVQFIHVDGEEKRDKESPSYYNIEEANKVLWVVKKLVKYNIPLSVITPYDAQVRYLRRLFEENNLDVEVNTVDGFQGRENEAIVISFVRTRKFGFLKDLRRLNVAITRAKRKLILIGNKNLLKQHPVYNEMLSWAES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU09</accession>
    <name>N6UU09_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Small ribosomal subunit protein uS19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 8" type="primary">rps19p</name>
        <name evidence="8" type="ORF">J422_05583</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 6">Protein S19 forms a complex with S13 that binds strongly to the 16S ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6 7">Belongs to the universal ribosomal protein uS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95839.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592676.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU09"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00277"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1089"/>
    <dbReference type="OrthoDB" id="30559at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.860.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.860.10">
        <property type="entry name" value="30s Ribosomal Protein S19, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00531">
        <property type="entry name" value="Ribosomal_uS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002222">
        <property type="entry name" value="Ribosomal_uS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020934">
        <property type="entry name" value="Ribosomal_uS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005713">
        <property type="entry name" value="Ribosomal_uS19_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023575">
        <property type="entry name" value="Ribosomal_uS19_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003121">
        <property type="entry name" value="PRK04038.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01025">
        <property type="entry name" value="uS19_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880">
        <property type="entry name" value="RIBOSOMAL PROTEIN S19P FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11880:SF2">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00203">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002144">
        <property type="entry name" value="Ribosomal_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00975">
        <property type="entry name" value="RIBOSOMALS19"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54570">
        <property type="entry name" value="Ribosomal protein S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00323">
        <property type="entry name" value="RIBOSOMAL_S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95839.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="151" mass="17545" checksum="FE83E40A88B872C0" modified="2013-06-26" version="1">MANPRARRKRKKQVVAKQVEFRYRGYTLEELQKMPLKEFIKLLPARQRRSLLRGLTPQQKKLAYKIKRARRLLNKGKEPRIIRTHCRDFIITPDMVGLTFGVYNGKEFVEVKVTPEMIGHYLGEFALTRKPVKHGAPGVGATRSSMFVPIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU98</accession>
    <name>N6UU98_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Anaerobic ribonucleoside-triphosphate reductase activating protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_05239</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000034">
        <property type="protein sequence ID" value="ENN95924.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592266.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU98"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00302"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1023"/>
    <dbReference type="OrthoDB" id="371936at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012840">
        <property type="entry name" value="NrdG2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02495">
        <property type="entry name" value="NrdG2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352:SF13">
        <property type="entry name" value="GLYCYL-RADICAL ENZYME ACTIVATING ENZYME YJJW-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PYRUVATE FORMATE-LYASE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01094">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="13"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95924.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="232" mass="26781" checksum="CB0991A64C3DD2AA" modified="2013-06-26" version="1">MRVAGIVDLSTIDYPKKCASVIFLSGCNMRCPYCHNLKYVLENGKEMSIEEIFDNIDFLFADAVVISGGEPTLQRDVVDLAKFFKERGFSVKLDTNGSNPNVVREMLDYIDYIAIDVKCSFSRYKEFVKYDGNIKEKILEIIKMCKNKVFVECRTTFVPKYLNEKDIEEIAKTVKDCDLYAIQQFDPKDAYDTSLKKISPPNEKELINLGKLARKYIKNVIVRTFDREILID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUP4</accession>
    <name>N6UUP4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_04288</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96059.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591746.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUP4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00666"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.839"/>
    <dbReference type="OrthoDB" id="146767at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008171">
        <property type="term" value="F:O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016461">
        <property type="entry name" value="COMT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001077">
        <property type="entry name" value="COMT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012967">
        <property type="entry name" value="COMT_dimerisation"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11746">
        <property type="entry name" value="O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08100">
        <property type="entry name" value="Dimerisation"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00891">
        <property type="entry name" value="Methyltransf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51683">
        <property type="entry name" value="SAM_OMT_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="O-methyltransferase dimerisation" evidence="5">
        <location>
            <begin position="21"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="O-methyltransferase C-terminal" evidence="4">
        <location>
            <begin position="144"/>
            <end position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96059.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="332" mass="38999" checksum="740A05110B12E82E" modified="2013-06-26" version="1">MSPRNILELFHYSYSDARIIYILKASLELRIFNYLEEYKTPEQISKELGLDSVLVDYLLKILYKLGLLEYKDGKYKNSEEANIFLNENSDLCLIDYLLYYFTYLRKWQNLKEILKGKREEISFKLIIKRLASGCKCWEVPKVVDYLSKYKEFETAKTLLDLGGGHGLYAVAFSRRFKNLKCYVFDLPEVIEEAEKLFLKGAKNVFTIKGDFFKDSLGNYDIIFSSYNPGGKNKEIAKKIYNSLNYNGLYINKQCFPEDDENLNNLLDGMEWHFFGSFKKGKYKFTFEGDLSLNEYIEYLKNLGFEILEVTDLKDIIGYSFYPTKLIVAKKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUQ7</accession>
    <name>N6UUQ7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">ATP-dependent dethiobiotin synthetase BioD</fullName>
            <ecNumber evidence="8">6.3.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">DTB synthetase</fullName>
            <shortName evidence="8">DTBS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Dethiobiotin synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">bioD</name>
        <name evidence="9" type="ORF">J422_04313</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA, also called 7,8-diammoniononanoate) to form a ureido ring.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(7R,8S)-7,8-diammoniononanoate + CO2 + ATP = (4R,5S)-dethiobiotin + ADP + phosphate + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15805"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:149469"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the dethiobiotin synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.3" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96064.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591756.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUQ7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00661"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.845"/>
    <dbReference type="OrthoDB" id="50320at2157"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00161"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004141">
        <property type="term" value="F:dethiobiotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042803">
        <property type="term" value="F:protein homodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03109">
        <property type="entry name" value="DTBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000292">
        <property type="entry name" value="ATP-dependent dethiobiotin synthetase BioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00336">
        <property type="entry name" value="BioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004472">
        <property type="entry name" value="DTB_synth_BioD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00347">
        <property type="entry name" value="bioD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43210:SF5">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13500">
        <property type="entry name" value="AAA_26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006755">
        <property type="entry name" value="DTB_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="8">
        <location>
            <position position="35"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="109"/>
            <end position="112"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="169"/>
            <end position="170"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96064.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="219" mass="24578" checksum="65E6FEA5545037F6" modified="2013-06-26" version="1">MIFITGTDTGVGKTYVSSILAKNLKKMGINVGYLKPIETGGREDTILLKNVLKTDDDINLMNPINLKLPLSPNIAFEVENYNISLDEIKEKIKNAYKTLKERHDFLIVEGAGGVCVPIKENFLMSDLIKFLNLPAIIVSRPNLGTINHTLLTVEHLRNKGIEIKGIIINCITPLEKVLYYEKTFETIEKFGKVKIIGIIEDEKRYNMDLSAIVGEKDGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUX5</accession>
    <name>N6UUX5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">S-adenosylmethionine decarboxylase proenzyme</fullName>
            <shortName evidence="15">AdoMetDC</shortName>
            <shortName evidence="15">SAMDC</shortName>
            <ecNumber evidence="15">4.1.1.50</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="15">S-adenosylmethionine decarboxylase beta chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="15">S-adenosylmethionine decarboxylase alpha chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="15" type="primary">speH</name>
        <name evidence="16" type="ORF">J422_03843</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Catalyzes the decarboxylation of S-adenosylmethionine to S-adenosylmethioninamine (dcAdoMet), the propylamine donor required for the synthesis of the polyamines spermine and spermidine from the diamine putrescine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 15">
            <text>S-adenosyl-L-methionine + H(+) = S-adenosyl 3-(methylsulfanyl)propylamine + CO2</text>
            <dbReference type="Rhea" id="RHEA:15981"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="4.1.1.50"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="15">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 15">Amine and polyamine biosynthesis; S-adenosylmethioninamine biosynthesis; S-adenosylmethioninamine from S-adenosyl-L-methionine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 15">Heterotetramer of two alpha and two beta chains arranged as a dimer of alpha/beta heterodimers.</text>
    </comment>
    <comment type="PTM">
        <text evidence="15">Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl group blocking the N-terminus of the alpha chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="14 15">Belongs to the prokaryotic AdoMetDC family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.50" evidence="15"/>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96159.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591365.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUX5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00400"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.753"/>
    <dbReference type="OrthoDB" id="114016at2157"/>
    <dbReference type="UniPathway" id="UPA00331">
        <property type="reaction ID" value="UER00451"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004014">
        <property type="term" value="F:adenosylmethionine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.110:FF:000001">
        <property type="entry name" value="S-adenosylmethionine decarboxylase proenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.110">
        <property type="entry name" value="S-adenosylmethionine decarboxylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00464">
        <property type="entry name" value="AdoMetDC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042286">
        <property type="entry name" value="AdoMetDC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003826">
        <property type="entry name" value="AdoMetDC_fam_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042284">
        <property type="entry name" value="AdoMetDC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016067">
        <property type="entry name" value="S-AdoMet_deCO2ase_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017716">
        <property type="entry name" value="S-AdoMet_deCOase_pro-enz"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03330">
        <property type="entry name" value="SAM_DCase_Bsu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33866:SF2">
        <property type="entry name" value="S-ADENOSYLMETHIONINE DECARBOXYLASE PROENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02675">
        <property type="entry name" value="AdoMet_dc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56276">
        <property type="entry name" value="S-adenosylmethionine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 15" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="4 15" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="9 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 15" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="11 15" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 15" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="10 15" id="KW-0704">Schiff base</keyword>
    <keyword evidence="6 15" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="8 15" id="KW-0865">Zymogen</keyword>
    <feature type="chain" id="PRO_5023448850" description="S-adenosylmethionine decarboxylase beta chain" evidence="15">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023448851" description="S-adenosylmethionine decarboxylase alpha chain" evidence="15">
        <location>
            <begin position="64"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate; via pyruvic acid" evidence="15">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor; for processing activity" evidence="15">
        <location>
            <position position="69"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor; for catalytic activity" evidence="15">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic); by autolysis" evidence="15">
        <location>
            <begin position="63"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser); by autocatalysis" evidence="15">
        <location>
            <position position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00056215"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00061583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN96159.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="123" mass="13492" checksum="A7B27D24ACC4222F" modified="2013-06-26" version="1">MLKFLGKHLILELWGCDEKALDDIEGIEKMLVDSVKACGATLICVKTHKFSPQGATGVAVLAESHISIHTWPELGYAAMDIFTCGSHVEPAKALPVIKEYLKPRHVEILDLKRGIRLGDASEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUZ1</accession>
    <name>N6UUZ1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl24p</name>
        <name evidence="4" type="synonym">rpl24</name>
        <name evidence="6" type="ORF">J422_03963</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Located at the polypeptide exit tunnel on the outside of the subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="4">One of two assembly initiator proteins, it binds directly to the 5'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96179.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591404.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUZ1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00422"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.777"/>
    <dbReference type="OrthoDB" id="10899at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06089">
        <property type="entry name" value="KOW_RPL26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000009">
        <property type="entry name" value="60S ribosomal protein L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01326_A">
        <property type="entry name" value="Ribosomal_uL24_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005756">
        <property type="entry name" value="Ribosomal_uL24_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041988">
        <property type="entry name" value="Ribosomal_uL24_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01080">
        <property type="entry name" value="rplX_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11143">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L26 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16906">
        <property type="entry name" value="Ribosomal_L26"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KOW" evidence="5">
        <location>
            <begin position="46"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96179.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="120" mass="14135" checksum="056BADF53B5DD4D6" modified="2013-06-26" version="1">MAFTKSKQPRKQRKALFNAPLHLRRKVMSAMLSKELKEKYGKNALPVRKGDVVRIMRGDYKGLEGEVVKVDLKRYRIFVEGATLKKQNGTEVYYPIHPSNVMIVKLYDKDERRFKHIKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVB3</accession>
    <name>N6UVB3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 13">4-hydroxy-tetrahydrodipicolinate reductase</fullName>
            <ecNumber evidence="10 13">1.17.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">J422_03029</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 18">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + NAD(+) + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35323"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 18">
            <text>(S)-2,3,4,5-tetrahydrodipicolinate + NADP(+) + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35331"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16845"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="EC" id="1.17.1.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the DapB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.17.1.8" evidence="10 13"/>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96299.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVB3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00153"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.594"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02274">
        <property type="entry name" value="DHDPR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022663">
        <property type="entry name" value="DapB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022664">
        <property type="entry name" value="DapB_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023940">
        <property type="entry name" value="DHDPR_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00036">
        <property type="entry name" value="dapB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836:SF0">
        <property type="entry name" value="4-HYDROXY-TETRAHYDRODIPICOLINATE REDUCTASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20836">
        <property type="entry name" value="DIHYDRODIPICOLINATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05173">
        <property type="entry name" value="DapB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000161">
        <property type="entry name" value="DHPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01298">
        <property type="entry name" value="DAPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0521">NADP</keyword>
    <keyword evidence="6 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydrodipicolinate reductase N-terminal" evidence="14">
        <location>
            <begin position="2"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydrodipicolinate reductase C-terminal" evidence="15">
        <location>
            <begin position="134"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00037922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00038983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="NCBIfam" id="TIGR00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF01113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF05173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN96299.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="226" mass="24882" checksum="166961DFFD425909" modified="2013-06-26" version="1">MIKVAVAGALGRMGREIIKAICEQEDMKLVAGFERKGHEDRGKDIGELIGLGTLGVKLSTADEMDNVLKETKPDVFVDFTIADACVNNVKIAAENKVNLVIGTTGFTEEQKKEILETIEKNNVAAVISENFAIGVNIFFKVVEFLTKYLKDYDIEIIEMHHRYKKDAPSGTALKIAEIIKSNRDVELVFGRYGKCERKENEVGVHALRGGDVVGDHIVIFAGDGRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVE3</accession>
    <name>N6UVE3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Metal-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03154</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96324.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590790.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVE3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00177"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.619"/>
    <dbReference type="OrthoDB" id="177623at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.110">
        <property type="entry name" value="Uncharacterised protein family UPF0058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002753">
        <property type="entry name" value="UPF0058"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036519">
        <property type="entry name" value="UPF0058_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42203">
        <property type="entry name" value="UPF0058 PROTEIN MJ1205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42203:SF2">
        <property type="entry name" value="UPF0058 PROTEIN MJ1205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01893">
        <property type="entry name" value="UPF0058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140371">
        <property type="entry name" value="Vng1086c-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96324.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="102" mass="12219" checksum="C1E1ADAE52C145C4" modified="2013-06-26" version="1">MNKDELIQLHQLLVYLKKYIEKNYGYFEEFKEYDELSIYPHHIHRTKAEHIYAIFLLSSIIAKILAENGKIPRSVHNLLKNSGEKIKKDIIRKRNKIQNVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVJ8</accession>
    <name>N6UVJ8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">MIP18 family-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02829</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96374.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590628.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVJ8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00115"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.555"/>
    <dbReference type="OrthoDB" id="371709at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.130">
        <property type="entry name" value="Fe-S cluster assembly (FSCA)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052339">
        <property type="entry name" value="Fe-S_Maturation_MIP18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034904">
        <property type="entry name" value="FSCA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002744">
        <property type="entry name" value="MIP18-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42831:SF1">
        <property type="entry name" value="FE-S PROTEIN MATURATION AUXILIARY FACTOR YITW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01883">
        <property type="entry name" value="FeS_assembly_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117916">
        <property type="entry name" value="Fe-S cluster assembly (FSCA) domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MIP18 family-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96374.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="94" mass="10495" checksum="EC7B58301CAC3C6B" modified="2013-06-26" version="1">MVSKEDVIKALRKVQDPHMGISIVDMGLVRDIEIDDNGNVKFIITPTNPACMSVLGMAMQAKKVVEELEGVKKVDVKVEGHFMEKEINEMLNEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVM8</accession>
    <name>N6UVM8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Molybdenum cofactor synthesis domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_02449</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MoaB/Mog family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96399.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590429.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVM8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00783"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.479"/>
    <dbReference type="OrthoDB" id="205337at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00886">
        <property type="entry name" value="MogA_MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012245">
        <property type="entry name" value="MoaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43232:SF2">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006443">
        <property type="entry name" value="MoaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01078">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="3">
        <location>
            <begin position="10"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96399.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="162" mass="18159" checksum="63A78225BA37FB63" modified="2013-06-26" version="1">MHKRLDVRYAIITVSDSRFEKFIKGESFKDESGEFLKNELKAKYHLLIPDEKDMIKGAVRYLINKDDIDCVVLTGGTGIAKRDNTVEAVKEIIEKELEGFKIAFQILSYNEVGFSSILSRATAGIVKDKLVYAIPGSINACKTAVKIIKEESGHIIGHLREI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVS2</accession>
    <name>N6UVS2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">protein adenylyltransferase</fullName>
            <ecNumber evidence="1">2.7.7.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_02375</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-tyrosyl-[protein] + ATP = O-(5'-adenylyl)-L-tyrosyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>O-(5'-adenylyl)-L-tyrosyl-[protein] + ATP = O-[5'-(adenylyl-(5'-&gt;3')-adenylyl)]-L-tyrosyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:66528"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17046"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:167160"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.108" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96434.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590347.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVS2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00356"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.467"/>
    <dbReference type="OrthoDB" id="18771at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="4">
        <location>
            <begin position="134"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00034531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00047518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96434.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="337" mass="39594" checksum="B7F3E04E45574AB1" modified="2013-06-26" version="1">MSMKIRTFIETVEGMYFAVVSYVHPKDRIIAFLRYVPYEYVDIPVDKSNIREINGKKYVKMADSDLAYKFLREKFSEYLFYDEVNDILIHAVPNDRIKNILKPEDRLKEILENSRNALEEKCRKLAIILEDYGVNLNKAGVTGSLLLKLNNPNSDIDFVVYGEEFNKARMALKEAIEDGKIEALTLEFWKKAYEKRIKDNSLTFEEFIFHEKRKYNRGVIDGTMFDLLFVRDRVDEKYGEKRYKGLGYISIEGKVLDDKLIFDSPAVYKIECYNDKEIKELCSFTHTYAGQCFKGEEFIARGKLEEVITKKGNYKRVVVGTKREAYNEYIKVKRCLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVU2</accession>
    <name>N6UVU2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ATPase RIL</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_02130</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96449.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590226.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVU2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01175"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.419"/>
    <dbReference type="OrthoDB" id="30658at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000152">
        <property type="entry name" value="ATP-binding cassette, sub-family E, member 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001546">
        <property type="entry name" value="RNase L inhibitor homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013283">
        <property type="entry name" value="RLI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009945">
        <property type="entry name" value="PRK13409.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19248">
        <property type="entry name" value="ATP-BINDING TRANSPORT PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01868">
        <property type="entry name" value="ABCEFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="41"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="73"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="337"/>
            <end position="549"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="3">
        <location>
            <begin position="554"/>
            <end position="584"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96449.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="584" mass="66557" checksum="C9F82BEF65DEA92F" modified="2013-06-26" version="1">MRLAIIDYERCQPKKCSLECIKYCPGVRMGEKTIVIDEKTGKPVISEVLCSGCGICVKRCPFKAISIIGLPEELTEDKIVHSYGQNRFKLFGLVIPREGVVGILGQNAIGKSTVLRILAGEIIPNLGKHGEEPSYDKVIEYFKGTELQSYFEMLKEKKIKAIHKVQYVDVLPKVVKGKVKDILKRIDEKGKFNYVVEKLELKNILDRTLDQLSGGELQRVAIAAAYLREGDIYFFDEPSSWLDVRQRFNMAKLVRELNKVIVVEHDLIVLDYLSDYVHIMYGEPGAYGIVSLPKSVRVGINEYLYGELREENIKFRREPIIFEKRAIIDYKNRPILLKYPNMKKSLGDFKLEVANGEIYKGEVIGILGPNGIGKTTFVKLLAGVIKPDDGEINEKVKVSYKPQYISPDYDGTVEDLLSSITNIHSSYYKSEIINPLQLEKLLDKEVNELSGGELQRVAIAACLSRDADIYLLDEPSAFLDVEQRLRVSKVIRRTADEKEAGIFVVDHDILFQDYISDRFIVFSGEPGRYGYASKPLNKREGANKFLKEMNITFRRDPETGRPRANKEGSQRDIMQKEKGEYYYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6UW03</accession>
    <name>N6UW03_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01755</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96504.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590109.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UW03"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00202"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.345"/>
    <dbReference type="OrthoDB" id="63958at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96504.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="17450" checksum="42F48A1B808B2179" modified="2013-06-26" version="1">MKSRIILLLAILFLCGCVAKKYPDVLYQEYHVVKVENLTIGNQKTVIVYQVKSEIKERASPYDLDADSKRDIGAIVYYVFKNTDADVVQIICYYAGSGEMQLYYKFKIDRKNAKLSGILDANENNLYEMALFSYSKLKYLGDLWINTRLKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UW07</accession>
    <name>N6UW07_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">L-fuculose phosphate aldolase</fullName>
            <ecNumber evidence="6">4.1.2.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">L-fuculose-1-phosphate aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">J422_01790</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>L-fuculose 1-phosphate = (S)-lactaldehyde + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:12933"/>
            <dbReference type="ChEBI" id="CHEBI:18041"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57846"/>
            <dbReference type="EC" id="4.1.2.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the aldolase class II family. AraD/FucA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.17" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96509.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590115.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UW07"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00208"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.352"/>
    <dbReference type="OrthoDB" id="18709at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008738">
        <property type="term" value="F:L-fuculose-phosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050197">
        <property type="entry name" value="Aldolase_class_II_sugar_metab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001303">
        <property type="entry name" value="Aldolase_II/adducin_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053406">
        <property type="entry name" value="Fuculose-P_aldolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040649">
        <property type="entry name" value="FucA_Meth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22789:SF0">
        <property type="entry name" value="3-OXO-TETRONATE 4-PHOSPHATE DECARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22789">
        <property type="entry name" value="FUCULOSE PHOSPHATE ALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00596">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01007">
        <property type="entry name" value="Aldolase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Class II aldolase/adducin N-terminal" evidence="12">
        <location>
            <begin position="4"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00020613"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96509.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="179" mass="20197" checksum="C2CAAB866EA23CD4" modified="2013-06-26" version="1">MNKKEFIKICHLLYERKYVVGSGGNVSVREGSKIYLTPTGSILGFLKEKDIAEMDIDGNVIKGNPTSERFMHLYIYKNREDVKAIIHTHSLISTFLSIIDKKIELLTPEGKFLNVGYVDYYEAGSLELAEAVAKRDEDVIILKNHGVVCLGDSLKSAYIKVEVLEEMAKLTLLKLIFQP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6UW95</accession>
    <name>N6UW95_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Chromatin associated protein KTI12</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01565</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96569.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UW95"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00440"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.308"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001717">
        <property type="term" value="P:conversion of seryl-tRNAsec to selenocys-tRNAsec"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.12.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013641">
        <property type="entry name" value="KTI12/PSTK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020024">
        <property type="entry name" value="L-seryl-tRNA_Sec_kinase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052648">
        <property type="entry name" value="Ser-tRNA(Sec)_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03574">
        <property type="entry name" value="selen_PSTK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20873">
        <property type="entry name" value="L-SERYL-TRNA(SEC) KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20873:SF0">
        <property type="entry name" value="L-SERYL-TRNA(SEC) KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08433">
        <property type="entry name" value="KTI12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96569.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="253" mass="29972" checksum="2A0E62BE2BEA2B36" modified="2013-06-26" version="1">MKCLKIILSEGQMLIILTGLPAVGKTEFSKKLAKELSNDVDVIVLGSDLIRESFPIWKVDYEPFIKESLFYLIDKALKKYWVIVDDTNYYNSIRRDLIKIAKKHNKRYAIIYLKAPLDVILKRNKEREKKVPDEVIINMYNKFDPPGKKYKWDEPFLEIDTTENIDYKKIAKILIEKSKEKIEIKKVIKEQSLLNKIDIETRKIINSYIKNKNLNKDEIIKIINLRKEFLKSLKNKNINYDEAIKEFKSLISR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWB1</accession>
    <name>N6UWB1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">NADH ubiquinone oxidoreductase, subunit 1 isolog</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_01465</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000010">
        <property type="protein sequence ID" value="ENN96589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589954.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWB1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01027"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.287"/>
    <dbReference type="OrthoDB" id="15253at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052561">
        <property type="entry name" value="ComplexI_Subunit1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43359">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43359:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="6" id="KW-0830">Ubiquinone</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="150"/>
            <end position="169"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="181"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="232"/>
            <end position="254"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="266"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="298"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="317" mass="35441" checksum="70AB0C4BEE3B9E69" modified="2013-06-26" version="1">MKELSALAIPILAFAISTYIPGIQRKIEARIQQRIGPSILSPGFWAFFKFIFKETQTPDAKLYKLYNNLPLLSIAVLSAILIVISLNNFLANLIFIVGLLKIEEMMYIILGSLSNSIMGCNMPFEDDCKGAKFINVIKYSLEQLSAIRNFKLITIGSFPFYLSLFLPFVNSKSIFINDLSGVFLFSLSGVFGAVCYFIGYLIMIKEYPFSISHTKADVIEGATLELMGKYRAFYLALTELLMITLGILFAVLYLNIIPNIEQPITILESFAIAIIFPILAAVVRAFSPLLLFKHIYPISYMATLIGVVGFILALLGW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWK6</accession>
    <name>N6UWK6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">4Fe-4S ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_00691</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589814.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWK6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00897"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.133"/>
    <dbReference type="OrthoDB" id="230142at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050157">
        <property type="entry name" value="PSI_iron-sulfur_center"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24960:SF79">
        <property type="entry name" value="PHOTOSYSTEM I IRON-SULFUR CENTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24960">
        <property type="entry name" value="PHOTOSYSTEM I IRON-SULFUR CENTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="4"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="52"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="83" mass="8861" checksum="0AD35A1F246FF8D1" modified="2013-06-26" version="1">MKAYELIVHEEKCHGCGNCVVSCPVNAKLPETWGGKGPYNEESVVILVENGTVKVVNPELCGGCGACVEACPVNAIELRFKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWM2</accession>
    <name>N6UWM2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">N-acetyl-gamma-glutamyl-phosphate reductase</fullName>
            <shortName evidence="7">AGPR</shortName>
            <ecNumber evidence="7">1.2.1.38</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">N-acetyl-glutamate semialdehyde dehydrogenase</fullName>
            <shortName evidence="7">NAGSA dehydrogenase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">argC</name>
        <name evidence="10" type="ORF">J422_00821</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the NADPH-dependent reduction of N-acetyl-5-glutamyl phosphate to yield N-acetyl-L-glutamate 5-semialdehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>N-acetyl-L-glutamate 5-semialdehyde + phosphate + NADP(+) = N-acetyl-L-glutamyl 5-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21588"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.2.1.38"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 3/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the NAGSA dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.38" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96734.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589834.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWM2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00871"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.162"/>
    <dbReference type="OrthoDB" id="372053at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00108"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003942">
        <property type="term" value="F:N-acetyl-gamma-glutamyl-phosphate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070401">
        <property type="term" value="F:NADP+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23934">
        <property type="entry name" value="AGPR_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17895">
        <property type="entry name" value="AGPR_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000014">
        <property type="entry name" value="N-acetyl-gamma-glutamyl-phosphate reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00150">
        <property type="entry name" value="ArgC_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050085">
        <property type="entry name" value="AGPR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023013">
        <property type="entry name" value="AGPR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058924">
        <property type="entry name" value="AGPR_dimerisation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000706">
        <property type="entry name" value="AGPR_type-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01850">
        <property type="entry name" value="argC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32338:SF10">
        <property type="entry name" value="N-ACETYL-GAMMA-GLUTAMYL-PHOSPHATE REDUCTASE, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32338">
        <property type="entry name" value="N-ACETYL-GAMMA-GLUTAMYL-PHOSPHATE REDUCTASE, CHLOROPLASTIC-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22698">
        <property type="entry name" value="Semialdhyde_dhC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01224">
        <property type="entry name" value="ARGC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 7" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0521">NADP</keyword>
    <keyword evidence="5 7 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="9">
        <location>
            <begin position="2"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="active site" evidence="7 8">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96734.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="334" mass="37667" checksum="915BD09B91A3C7B6" modified="2013-06-26" version="1">MDVAIVGASGYTGGELLRLLAFHKEANVVSITSRRFKGEKVYKVHPHLRKFYDLTFTEDVKDADVVFTALPHGTSMKVVPEFLERGMRVIDLSGDYRFEDLSIYEKYYKIKHTGLPNVKIVYGLTEIYREEIKKAQLISNPGCFPTGAILALYPLVKEGIIEENIIIDAKTGITGAGANPSERTHFPFVNEDIIAYNITTHRHKPEIEKELRKVNNKIKVSFVPHLSPINRGILTTIHGFLKEKIDNEELIEIYKKFYKNDIFIRIYKDIPKLSYVRGSNFCDIGGFGIDGKNLIVISAIDNLIKGASGQAIQNMNVMFGIDESEGLMNVPINP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWR4</accession>
    <name>N6UWR4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Extracellular ligand-binding receptor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_00441</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96784.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589770.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWR4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00692"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.85"/>
    <dbReference type="OrthoDB" id="21336at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd06346">
        <property type="entry name" value="PBP1_ABC_ligand_binding-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001828">
        <property type="entry name" value="ANF_lig-bd_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051010">
        <property type="entry name" value="BCAA_transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028082">
        <property type="entry name" value="Peripla_BP_I"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483:SF40">
        <property type="entry name" value="HISTIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30483">
        <property type="entry name" value="LEUCINE-SPECIFIC-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01094">
        <property type="entry name" value="ANF_receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53822">
        <property type="entry name" value="Periplasmic binding protein-like I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-0675">Receptor</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Receptor ligand binding region" evidence="5">
        <location>
            <begin position="45"/>
            <end position="386"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96784.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="406" mass="45338" checksum="CCBDB169B1B82023" modified="2013-06-26" version="1">MGLILITFLAGCTQTGEKAGGNIKEVKLGLLVDLSGSLATYGQNEKHACEIAEEKVNEYFKEHNIPYKVKLYVEDTKADPKICLDKVQALHAIGVNAFIGPMSSSEVKNVKSYSLSNKVIFVSQSSTADPKILGFMSPEEKKYIFRFVPTDSFQGKAIGDLAKELNLKNVIILYRKDAWGEGLKDAIVKKMKDNGINIINIIPYDPQINDWSPIIQTLSDNIKDKGEDTGVVFVGYEEGATLLSQINDNSPILNHIWIGTDGVANSEKIIKEVKDKAVKVKLYSTMFKSESEAAKELEKIFKEKNYGNIDQYALNAYDAFWVVSLAYVDMLNKTGKYDADVLSNMIKEVTKKYSEGVYGVEPVSGYIELNEWNDRASGDYGIFMVTEKGWKLAGIWHSKDGKIEWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWX4</accession>
    <name>N6UWX4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Peptidase S24/S26A/S26B, conserved region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_00315</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589750.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWX4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01100"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.63"/>
    <dbReference type="OrthoDB" id="4822at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006465">
        <property type="term" value="P:signal peptide processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06530">
        <property type="entry name" value="S26_SPase_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036286">
        <property type="entry name" value="LexA/Signal_pep-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019533">
        <property type="entry name" value="Peptidase_S26"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001733">
        <property type="entry name" value="Peptidase_S26B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10806:SF6">
        <property type="entry name" value="SIGNAL PEPTIDASE COMPLEX CATALYTIC SUBUNIT SEC11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51306">
        <property type="entry name" value="LexA/Signal peptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="197"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="218" mass="25600" checksum="91D899A33440AD0A" modified="2013-06-26" version="1">MRVVIIKKEKIIEWIVFLIVLFLIWSHVNVVVSNSMYPIMKRGDLVIVENAGFEFNPNDVKVGDIVVYSAHWDAYQYILYAISQKLGYDFNNTLFIFNRGDDKLEAKVLGKLKTDVGEFVIINFSTPKLFEKPVIHRVIDKVEFDNRTYFIIKGDNNYMHDPELVSINQIKQRVLTIDGHPLIIPYLGYLSIYAKEYWYIIVLIIIFDILYKQLRGKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6UZX7</accession>
    <name>N6UZX7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cohesin domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_07077</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000072">
        <property type="protein sequence ID" value="ENN95578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594878.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000072.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UZX7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00455"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1369"/>
    <dbReference type="OrthoDB" id="66078at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002102">
        <property type="entry name" value="Cohesin_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00963">
        <property type="entry name" value="Cohesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="Carbohydrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cohesin" evidence="1">
        <location>
            <begin position="19"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="179" mass="20291" checksum="477E4B626F839B30" modified="2013-06-26" version="1">MKKIAIVMLFLLLTNLYAVNLKIDTPKNVKVGEEFNLTIDVINDKKISGFECFITIPKDKIKIISIEDNKTIKNKAGNFYFSNFSNSKAFVKFALFDKPINSNFRLLNLKLRAIRSGDAKIIINAVASDDDGNCIFRENKEIEIKIIGGNSEGNKSEKENFFDVLLKTILNFIKLLFGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6UZY1</accession>
    <name>N6UZY1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF483 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_07052</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000069">
        <property type="protein sequence ID" value="ENN95583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594760.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000069.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UZY1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01088"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1365"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007556">
        <property type="entry name" value="DUF483"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04467">
        <property type="entry name" value="DUF483"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17648" checksum="D63737885A5FD92C" modified="2013-06-26" version="1">MMEDILKKILQMREGKSEFKKLEENIKNMDDIKFEYLINRLKTQIEIVNKYKPKVRPALDPVISFELGVYRRLDDYEIGKLLNYPLCCIKSFSEDFRIAIDREHLKEAKEINTYAIVITSGFIPCSLKCKMAMKNGLLGYMDEEEFKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6V017</accession>
    <name>N6V017_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11 12">Homoserine kinase</fullName>
            <shortName evidence="11">HK</shortName>
            <shortName evidence="11">HSK</shortName>
            <ecNumber evidence="3 11 12">2.7.1.39</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">thrB</name>
        <name evidence="15" type="ORF">J422_06807</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-homoserine + ATP = O-phospho-L-homoserine + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13985"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
            <dbReference type="ChEBI" id="CHEBI:57590"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.39"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the GHMP kinase family. Homoserine kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.39" evidence="3 11 12"/>
    <dbReference type="EMBL" id="APMM01000062">
        <property type="protein sequence ID" value="ENN95633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594212.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V017"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01090"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1321"/>
    <dbReference type="OrthoDB" id="28273at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00064"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004413">
        <property type="term" value="F:homoserine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00384">
        <property type="entry name" value="Homoser_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000870">
        <property type="entry name" value="Homoserine_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002288">
        <property type="entry name" value="PRK01212.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00191">
        <property type="entry name" value="thrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF1">
        <property type="entry name" value="HOMOSERINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000676">
        <property type="entry name" value="Homoser_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00958">
        <property type="entry name" value="HOMSERKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 11 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="6 11 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="13">
        <location>
            <begin position="51"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="14">
        <location>
            <begin position="203"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="78"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR00191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN95633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="286" mass="31404" checksum="70C56BA66842AF1B" modified="2013-06-26" version="1">MKFKAPCTSANLGVGFDVFGLCLNEPYDIIEIKEDEGIIIESDLNIEQKKNVAYVVAKKMIEDFKLKGVRIKIKKGVKPGSGLGSSAASSAGTAVAINELYNLKLSKLELVEYASLGEELASGDKHYDNVAPAIFGGFTIIANKKPLDIIKIDVDLNIIIALPDITINTKKAREILPKCYKLEDVVNNLGKASAVVYAFLKDKERLKYIMDDKLAEPYRGKLIPNYFEVKNILKDKVYAISISGSGPAIIAFPKEDCANEVKEVLKEYYGNVIETCVGKGVECLKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0A5</accession>
    <name>N6V0A5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">SigmaK-factor processing regulatory BofA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06090</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593239.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0A5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00537"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1186"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010001">
        <property type="entry name" value="BofA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07441">
        <property type="entry name" value="BofA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="80" mass="8940" checksum="A95C58818E2E4805" modified="2013-06-26" version="1">MDFYFLAMIGILILVAILIIKLTLKIIRYLAINTAIGIILIFILKFLGINIKLNLINLLIIAIGGVVGVFILLILHFLHI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0M6</accession>
    <name>N6V0M6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA pseudouridine synthase A</fullName>
            <ecNumber evidence="4">5.4.99.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridine(38-40) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA pseudouridylate synthase I</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">tRNA-uridine isomerase I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4 9" type="primary">truA</name>
        <name evidence="9" type="ORF">J422_05459</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 7">
            <text>uridine(38/39/40) in tRNA = pseudouridine(38/39/40) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:22376"/>
            <dbReference type="Rhea" id="RHEA-COMP:10085"/>
            <dbReference type="Rhea" id="RHEA-COMP:10087"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.12"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 7">Belongs to the tRNA pseudouridine synthase TruA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.12" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000041">
        <property type="protein sequence ID" value="ENN95868.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592537.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0M6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01401"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1066"/>
    <dbReference type="OrthoDB" id="25720at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160147">
        <property type="term" value="F:tRNA pseudouridine(38-40) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.580:FF:000032">
        <property type="entry name" value="tRNA pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.660">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.580">
        <property type="entry name" value="Pseudouridine synthase I, catalytic domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00171">
        <property type="entry name" value="TruA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001406">
        <property type="entry name" value="PsdUridine_synth_TruA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020097">
        <property type="entry name" value="PsdUridine_synth_TruA_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020095">
        <property type="entry name" value="PsdUridine_synth_TruA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020094">
        <property type="entry name" value="TruA/RsuA/RluB/E/F_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00071">
        <property type="entry name" value="hisT_truA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11142:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01416">
        <property type="entry name" value="PseudoU_synth_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001430">
        <property type="entry name" value="tRNA_psdUrid_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4 7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Pseudouridine synthase I TruA alpha/beta" evidence="8">
        <location>
            <begin position="118"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="4 5">
        <location>
            <position position="50"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4 6">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009375"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001430-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95868.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="258" mass="30692" checksum="3505C3534B2B8CFA" modified="2013-06-26" version="1">MYILKIAYDGRYSFQQQPSKDTVCDKLLDVLDKLDFLKEYKIIYSGGRTDKGVSALGNFVVLKLKREPILSYINHHLKDYGIWILGYKEIEKIPKVRYRHYRYILPNIGYDISLIKEASKKFIGKHSFHNLCKRDRSKDRDPVREIYDIKIIENEFFITIDIIGKSFLWHMVRKIVGVFDAIGRGEKDIEWIDKLLDPNHKEGVRTFPAEGLILIEAKVDIDYNYDSYSIKKFLEYWQERYKFYIMKMGISRSFITTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0P9</accession>
    <name>N6V0P9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Succinate--CoA ligase [ADP-forming] subunit alpha</fullName>
            <ecNumber evidence="5">6.2.1.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Succinyl-CoA synthetase subunit alpha</fullName>
            <shortName evidence="5">SCS-alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">sucD</name>
        <name evidence="10" type="ORF">J422_05384</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 8">Succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of either ATP or GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The alpha subunit of the enzyme binds the substrates coenzyme A and phosphate, while succinate binding and nucleotide specificity is provided by the beta subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GTP + succinate + CoA = succinyl-CoA + GDP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:22120"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>succinate + ATP + CoA = succinyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:17661"/>
            <dbReference type="ChEBI" id="CHEBI:30031"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5 8">Carbohydrate metabolism; tricarboxylic acid cycle; succinate from succinyl-CoA (ligase route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 8">Heterotetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 7">Belongs to the succinate/malate CoA ligase alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000038">
        <property type="protein sequence ID" value="ENN95888.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592443.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0P9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00461"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1051"/>
    <dbReference type="OrthoDB" id="55711at2157"/>
    <dbReference type="UniPathway" id="UPA00223">
        <property type="reaction ID" value="UER00999"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009361">
        <property type="term" value="C:succinate-CoA ligase complex (ADP-forming)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004776">
        <property type="term" value="F:succinate-CoA ligase (GDP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.261:FF:000006">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000205">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01988">
        <property type="entry name" value="Succ_CoA_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017440">
        <property type="entry name" value="Cit_synth/succinyl-CoA_lig_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033847">
        <property type="entry name" value="Citrt_syn/SCS-alpha_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005810">
        <property type="entry name" value="CoA_lig_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004230">
        <property type="entry name" value="PRK05678.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01019">
        <property type="entry name" value="sucCoAalpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117:SF2">
        <property type="entry name" value="SUCCINATE--COA LIGASE [ADP_GDP-FORMING] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11117">
        <property type="entry name" value="SUCCINYL-COA LIGASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02629">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001553">
        <property type="entry name" value="SucCS_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01798">
        <property type="entry name" value="SCOASYNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01216">
        <property type="entry name" value="SUCCINYL_COA_LIG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00399">
        <property type="entry name" value="SUCCINYL_COA_LIG_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 5 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 8" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="CoA-binding" evidence="9">
        <location>
            <begin position="3"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-phosphohistidine intermediate" evidence="5 6">
        <location>
            <position position="245"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="16"/>
            <end position="19"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="95"/>
            <end position="97"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>substrate</name>
            <note>ligand shared with subunit beta</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001553-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000677"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95888.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="289" mass="30724" checksum="069AB0308AD0A876" modified="2013-06-26" version="1">MILLDENTRAIVQGITGRQGSFHTKKMLEAGTKIVAGVTPGKGGKEVHGVKVYDTVKRAVEEHDANASVVFVPAPHAKDAVYEAIDAGIELIVVITEHIPIHDTMEFVNYAEELGVKIIGPNTPGIASPRVGKLGIIPMEVLKEGDIGIVSRSGTLTYEIANQIKNSGFGVSTCVGIGGDPIVGLGFREILEMFENDCETEVVVLIGEIGGSAEERAAEYIKKMKKPVIAYIAGKSAPKGKRMGHAGAIIEGEKGTAESKIKALKNVGAYIANNISDIPKILMEVKKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0S9</accession>
    <name>N6V0S9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Peptidase U62 modulator of DNA gyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_05199</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000034">
        <property type="protein sequence ID" value="ENN95918.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592260.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0S9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00309"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1015"/>
    <dbReference type="OrthoDB" id="84520at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047657">
        <property type="entry name" value="PmbA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43421">
        <property type="entry name" value="METALLOPROTEASE PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43421:SF1">
        <property type="entry name" value="METALLOPROTEASE PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E N-terminal" evidence="1">
        <location>
            <begin position="14"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E central" evidence="3">
        <location>
            <begin position="101"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="2">
        <location>
            <begin position="201"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95918.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="407" mass="46152" checksum="B4F59CAB4DE07DDC" modified="2013-06-26" version="1">MDIVKLGEKYGYDVEIFMQKGVSVGVELDGENVDSFEYHSSIGYGIRVLKNNKVGFAYGNVLNEELLKKAMKNLVYDEYSSLAEPDKYKEPKGLFNKEVCNLTEEELLDRLLEMKEINANILSGGVYKSISYFRLINSYGLDVEERQTFFSANISIMLNGETAYEYKTDHKLFNTREVSERAVELAKNSANGKKIRYKGIIILSPRALSNLLYYTLYPAFSAENVQRNRSYLKDKLGEEVFSKNITIVDDNSLDYALYSEKCDAEGVKSQRTVLVENGVLKSYLYDIKRANVESKESTSNCSRGYSTLPSIAPTNFIIEEKEKNDFDEYVYINDIIGAHTSNPITGDFSIEIKNSYLYKKGEIIGLKKGLFSGNIFKLFKNAIPLNDSEQRGHLISPSIAFEGEIIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0W0</accession>
    <name>N6V0W0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">dCTP deaminase, dUMP-forming</fullName>
            <ecNumber evidence="3">3.5.4.30</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Bifunctional dCTP deaminase:dUTPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">DCD-DUT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">dcd</name>
        <name evidence="4" type="ORF">J422_04855</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Bifunctional enzyme that catalyzes both the deamination of dCTP to dUTP and the hydrolysis of dUTP to dUMP without releasing the toxic dUTP intermediate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>dCTP + 2 H2O = dUMP + NH4(+) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19205"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
            <dbReference type="ChEBI" id="CHEBI:246422"/>
            <dbReference type="EC" id="3.5.4.30"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the dCTP deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.30" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95943.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592058.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0W0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00519"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.951"/>
    <dbReference type="OrthoDB" id="33242at2157"/>
    <dbReference type="UniPathway" id="UPA00610">
        <property type="reaction ID" value="UER00667"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033973">
        <property type="term" value="F:dCTP deaminase (dUMP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006226">
        <property type="term" value="P:dUMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00146">
        <property type="entry name" value="dCTP_deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02274">
        <property type="entry name" value="dCTP_deam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680:SF3">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22769">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 3" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="3">
        <location>
            <position position="144"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="116"/>
            <end position="121"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="142"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>dCTP</name>
            <dbReference type="ChEBI" id="CHEBI:61481"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for bifunctional activity" evidence="3">
        <location>
            <begin position="131"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95943.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="201" mass="23401" checksum="C25438A51A59FA0D" modified="2013-06-26" version="1">MILSDKDILKLINENKLIIEPFNREHLGPASYDVTLGNEFIVYEDEVYDLFNDLNYKRIKIKNSILICPINYNLTEEKIEYFKEKYNVDYVSKAILGTTNEYIELPNDITSFYQGRSSLGRLFLTSHQTAGFIDAGFRGKITLEIVAHDKPVILYPNMRIGQLIFFKLNSPAEIGYCEKKNSKYAYQRTVMPSLIKRDFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0X9</accession>
    <name>N6V0X9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04930</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95958.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0X9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00505"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.966"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01310">
        <property type="entry name" value="TatD_DNAse"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46124">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46124:SF2">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005902">
        <property type="entry name" value="DNase_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="146"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR005902-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95958.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="200" mass="22940" checksum="1D77B1B9F3AA772D" modified="2013-06-26" version="1">MSKYVDAHCHIEDKAFNKNRDEVVKRAKENNVIIITSGASFGGCVRALNCKRLYDIYLTLGYHPSRVNADDKIINKVYNLIKENEKEILAVGEIGMDVKIENIKRQEEIFKKFLELSKEINKPIVAHARGFEERIFKLSDQPIMFHCYSGSLELAKEIGESGNLISISTLVCFSEHHKKLVEKLDIDYITTETDSLIYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1D6</accession>
    <name>N6V1D6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Integral membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_04333</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the TMEM19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96068.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591766.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1D6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00656"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.849"/>
    <dbReference type="OrthoDB" id="28948at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002794">
        <property type="entry name" value="DUF92_TMEM19"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00297">
        <property type="entry name" value="TIGR00297 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13353:SF5">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN 19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01940">
        <property type="entry name" value="DUF92"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="80"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="210"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96068.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="228" mass="24488" checksum="2DF49FCC33D3BC29" modified="2013-06-26" version="1">MNLLLSTIIVIILALLVKKSKCLDNKGILLASIMAFILIYFCGLEYLIVLLSFFILGSLVSRVGLKEKKKKKIAETCRGLNNVIANGLIPMFFAILSIKIPIFLIAYISSIAAATSDTFSSEIGVLSKEKPRLVTTFEVVEKGTDGAITLLGTFAGLFGAFLIALIGTILFNNIKLLLPVTLAGLIGNLADSLFGALFERKGLASNEHTNLVATLVGGVFGIIFYLIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1E8</accession>
    <name>N6V1E8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">A-type ATP synthase subunit F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">atpF</name>
        <name evidence="7" type="ORF">J422_04418</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the V-ATPase F subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96083.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591798.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1E8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00640"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.866"/>
    <dbReference type="OrthoDB" id="24971at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10580">
        <property type="entry name" value="ATPase, V1 complex, subunit F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00312">
        <property type="entry name" value="ATP_synth_F_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008218">
        <property type="entry name" value="ATPase_V1-cplx_f_g_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022944">
        <property type="entry name" value="ATPase_V1-cplx_fsu_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036906">
        <property type="entry name" value="ATPase_V1_fsu_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003047">
        <property type="entry name" value="PRK03957.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01990">
        <property type="entry name" value="ATP-synt_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159468">
        <property type="entry name" value="AtpF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="3 6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96083.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="98" mass="11198" checksum="0C89E160DD240B07" modified="2013-06-26" version="1">MKIAVIGDRETAIGFRLAGLSEVYEVKNDDEAIDILNDLNNKEEIAFIIITERISEKIKDKIRNINKVIVEIPDKNGKLRREDPIKELIRKAIGVVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1M3</accession>
    <name>N6V1M3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Small ribosomal subunit protein uS8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rps8p</name>
        <name evidence="8" type="synonym">rps8</name>
        <name evidence="10" type="ORF">J422_03983</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">One of the primary rRNA binding proteins, it binds directly to 16S rRNA central domain where it helps coordinate assembly of the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 8">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8 9">Belongs to the universal ribosomal protein uS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96183.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591412.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1M3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00426"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.781"/>
    <dbReference type="OrthoDB" id="5670at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.30:FF:000001">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S15a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01302_A">
        <property type="entry name" value="Ribosomal_uS8_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000630">
        <property type="entry name" value="Ribosomal_uS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047863">
        <property type="entry name" value="Ribosomal_uS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035987">
        <property type="entry name" value="Ribosomal_uS8_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003115">
        <property type="entry name" value="PRK04034.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11758">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S15A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00410">
        <property type="entry name" value="Ribosomal_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56047">
        <property type="entry name" value="Ribosomal protein S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00053">
        <property type="entry name" value="RIBOSOMAL_S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="6 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96183.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="130" mass="14501" checksum="B27693E5D0C3C102" modified="2013-06-26" version="1">MSLMDPLANALNHLSNCEKVGKKVAYIKPASKLIGRVLKVMQDYGYIGQFEFIDDGKAGVFKVELIGKINKCGAIKPRFPVKKMGYEKFEKRYLPARDFGILIVSTTQGVMSHEEAKKKGLGGRLLAYVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1Q8</accession>
    <name>N6V1Q8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Membrane protein 6-pyruvoyl-tetrahydropterin synthase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03463</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000022">
        <property type="protein sequence ID" value="ENN96223.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1Q8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01341"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.675"/>
    <dbReference type="OrthoDB" id="307366at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="75"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="105"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="179"/>
            <end position="199"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="250"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="282"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="547"/>
            <end position="565"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96223.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="574" mass="67569" checksum="403DC98BA10B4651" modified="2013-06-26" version="1">MNKLMETFTDSKIAQLYAGLLYMINPYTYIRIVVGHWLILFAYAILPLAIKYFIELLNKKKLKSIIKFSLITTLVAFNAHTLFIAFLIFLIVLAFYIYKNKSLEILKPIILSSIIFLILNTYWLIPTITAEKQSIVVNISKADLYVFAPHIESFSALFTLASMHGFWRPAYIYAKDFLPFWPILFIFILFLAVHGFTSYCNNKKLGIYVKAFTLIWFIGLILASGILSPFSEIFEFLFNHILLFKGMRDTHKFVTMLCLSYSFLGALGLAEIEKSLKNKKPIIKNALILAILLIPLIYSFTFFNGFAHQIKPTDFPKDWYEVNEFLNKDKDDFRVLFFPWHLYMDFHWVPNKDKRIANPAPYFFDKEVISAKNIEVPGIYRQVYTPYQVYIDNLLSKKDKIKNFGHLVTPLGIKYILLTKEADYKNYFFLFNQSDLKLIMETKNFYVFKNEAFKGLIYSVNSSNWNLTNEKIDERDYKKLNYTKVSPVEYIIKDKPLRYIIFTEEYSKDWKFNGKEPLKAYGVVNAYKYDDEKEMKIVYERFYKICLPSYIISLLAFILCMGYLVKDKVLNHGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1T9</accession>
    <name>N6V1T9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Ribonuclease</fullName>
            <ecNumber evidence="14">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">rnhB</name>
        <name evidence="16" type="ORF">J422_03318</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 14">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18 13 14">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 14">Belongs to the RNase HII family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="14"/>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96258.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1T9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01050"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.650"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.460:FF:000001">
        <property type="entry name" value="Ribonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.460">
        <property type="entry name" value="Ribonuclease hii. Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023160">
        <property type="entry name" value="RNase_HII_hlx-loop-hlx_cap_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF23">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="11 14 16" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12" id="KW-0464">Manganese</keyword>
    <keyword evidence="9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 14" id="KW-0540">Nuclease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-2" evidence="15">
        <location>
            <begin position="1"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00007383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN96258.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="79" mass="9280" checksum="5852EC9886A4E899" modified="2013-06-26" version="1">MISIFVVSAASIIAKVIRDNIINEYKKEFGDFGSGYPSDIKTVEFLKRYYEIHNKLPPIAREKWKTCKRLKGETLDRWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1V4</accession>
    <name>N6V1V4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphomethylpyrimidine synthase</fullName>
            <ecNumber evidence="10">4.1.99.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Hydroxymethylpyrimidine phosphate synthase</fullName>
            <shortName evidence="10">HMP-P synthase</shortName>
            <shortName evidence="10">HMP-phosphate synthase</shortName>
            <shortName evidence="10">HMPP synthase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Thiamine biosynthesis protein ThiC</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">thiC</name>
        <name evidence="11" type="ORF">J422_03403</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + S-adenosyl-L-methionine = 4-amino-2-methyl-5-(phosphooxymethyl)pyrimidine + CO + 5'-deoxyadenosine + formate + L-methionine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:24840"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58354"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="EC" id="4.1.99.17"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="10">Binds 1 [4Fe-4S] cluster per subunit. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the ThiC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.17" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96273.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590994.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1V4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01066"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.668"/>
    <dbReference type="OrthoDB" id="335406at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070284">
        <property type="term" value="F:phosphomethylpyrimidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.540:FF:000001">
        <property type="entry name" value="Phosphomethylpyrimidine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.540">
        <property type="entry name" value="Radical SAM ThiC family, central domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00089">
        <property type="entry name" value="ThiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037509">
        <property type="entry name" value="ThiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038521">
        <property type="entry name" value="ThiC/Bza_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002817">
        <property type="entry name" value="ThiC/BzaA/B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009895">
        <property type="entry name" value="PRK13352.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00190">
        <property type="entry name" value="thiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557:SF1">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30557">
        <property type="entry name" value="THIAMINE BIOSYNTHESIS PROTEIN THIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01964">
        <property type="entry name" value="ThiC_Rad_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00407">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01114">
        <property type="entry name" value="phosphomethylpyrimidine_syntha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00113">
        <property type="entry name" value="Radical_SAM_Phosphomethylpyrim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 10" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 10" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="5 10" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="184"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="225"/>
            <end position="228"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="268"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="332"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="408"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="415"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96273.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="425" mass="46972" checksum="93087B4062937B7F" modified="2013-06-26" version="1">MTQITEAKKGNITEEMKVVAKNEGIDEEKLRRYIAKGYVVIPKNINRDTKPVGIGKNLRTKVNANIGTSPDFVNIKLEVEKAKVAVKYGADTVMDLSTGGDLSKIRKAIMEAVNVPIGTVPIYEAAKIAKEKFGKVIDMNEDIIFKVIENQAKEGVDFMTLHCGVTKQSVDRLKKSKRVLGVVSRGGAFLTAYIIYHNEENPLYKNFDYLLEILKEYDITISLGDGLRPGAILDNTDRAQIEELLILGELVERCREFGVQAMVEGPGHVPINNIEANIRLQKSVCKNAPFYVLGPIVTDIALGYDHIAAAIGGALAGYYGADFLCYVTPAEHLRLPTIEDVKLGVIATKIAAQAADVAKGNRLALELEKEMAFARKNHDWEKQFNLAIDGEKARKMKEEIPSKDKKACSICGDFCALLLVEKFLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1W4</accession>
    <name>N6V1W4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Reverse gyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_03231</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000019">
        <property type="protein sequence ID" value="ENN96283.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1W4"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160097">
        <property type="term" value="F:reverse gyrase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005736">
        <property type="entry name" value="Reverse_gyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040569">
        <property type="entry name" value="Znf_Rg"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43505">
        <property type="entry name" value="REVERSE GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43505:SF1">
        <property type="entry name" value="REVERSE GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17915">
        <property type="entry name" value="zf_Rg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52036">
        <property type="entry name" value="ZF_RG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="RG N-terminal-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS52036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96283.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="46" mass="5332" checksum="BE0326C0261834A9" modified="2013-06-26" version="1" fragment="single">MVVPMIFKEMCPNCNGEIESERLKIGVCKKCLKEDKEVEKTELCKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6V249</accession>
    <name>N6V249_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02719</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96358.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590596.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V249"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00096"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.533"/>
    <dbReference type="OrthoDB" id="65984at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019249">
        <property type="entry name" value="DUF2226"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09987">
        <property type="entry name" value="DUF2226"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96358.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="377" mass="44473" checksum="3FD227A9D2A55DE2" modified="2013-06-26" version="1">MIKILEGEFLKSLKDIKLEDATREVNTGYIQIYTTEDDKIIYGYIFVENGEVVGYYYTDNKSVEDMGNPEKVLELFKKENKEIELYKYEKDKLNLLEWLYPEIFNIKKKAKAKEEIIIEEKEEDYINIKLGIPLPIPAFTNVKDFKKYLNMFEYSIVNVYRKSKDDLENGYIVYKKDIPIAAAYECKKGVLFGNKAYKKIKEMLDDKNSIIDIYPYDNKKLNILLEVYPQMEMGYEEKVEEEKKISEFLKKDLENLSRDELLKKLGIKEPDEDWVDGIIKDFLKPSMEDLLDLKTLIENEIKKSLKELDIVEDVLVSININWENGNYIVVGHVNVIRKKLFGLIKKTLKKETVEEIIEKCVRKYLSNYIIKVDISIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6V288</accession>
    <name>N6V288_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02909</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96388.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590657.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V288"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00130"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.571"/>
    <dbReference type="OrthoDB" id="116994at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96388.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="115" mass="13800" checksum="6D861C7ECFFF0D8B" modified="2013-06-26" version="1">MENWKLTYKVKCFNCGKEVDQIIEIFPNQAFITCSNCGAKRYYIIRRVGIEDEKIINEEREKEHKYEPWFLEKKAVCFNCKNEAIQDIVVTESKLIVRCRHCGFTRVYLFHILEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2W6</accession>
    <name>N6V2W6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH cro/C1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01560</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589971.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2W6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00439"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.307"/>
    <dbReference type="OrthoDB" id="11138at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004451">
        <property type="entry name" value="MJ0586"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058562">
        <property type="entry name" value="MJ0586_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00270">
        <property type="entry name" value="multiprotein bridging factor aMBF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26602">
        <property type="entry name" value="HVO_2718_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="81"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="160" mass="18899" checksum="17D9BC2C4C5BD6DE" modified="2013-06-26" version="1">MQMCELCGKLTDKLYKVIIEGSEMMVCEKCKKFGKTPKTYSRIGKKIPTRDVKTEKRIVKRPRRDLFDKLVELREDYGDVIREAREKLGLSIEELARRLKIKATVLQKFERYELEPNEKEIKLLEKELKINLLANPQEETIESSSEEESFTLGHFLKFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPB6</accession>
    <name>N6VPB6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MCM family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_06311</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000051">
        <property type="protein sequence ID" value="ENN95720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593437.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPB6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01271"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1228"/>
    <dbReference type="OrthoDB" id="6747at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006270">
        <property type="term" value="P:DNA replication initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17706">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008047">
        <property type="entry name" value="MCM_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01660">
        <property type="entry name" value="MCMPROTEIN4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="7">
        <location>
            <begin position="262"/>
            <end position="464"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="660" mass="75648" checksum="0D9B26B971E5B243" modified="2013-06-26" version="1">MNLEKFKDLLIAYLRNFHQDDIIMEKDRVNIDLKKLYYFGLMEFVEFTVNHPYEAINIIKECYKEIYFTLKGEELEDIIIELNNIPEVFKRNYTIEKIKSKDIGKLVEFEGIIVMASKIKPMLKKGFYICPKCGRKSVKEIDFLKDCEEVICKCGEVMELIDDRSTYVDFQEIKVQQPLDLMSNPEEPPKYITVFLENSPGIYAGRVKVLGVPIKVRKSKKLPIYDLYVKAISCNVLDEGAKISISEEDIKNIKKLSKRKDIVDILSDRLIPEIKGYSTIKKAVFLQQIKGVKKPNKRADIHILLITDPGIGKTVILRNIAEIPGNVYGSVTTATGVGLTAAVVKEKTEIGEDNWVIKPGLLVKANKGTACIDELTVNKEILSYVLEAMESQTIHVSKGGINAKLPAECAVLAACNPRWGRFDPEKSIAEQINIPAPLLSRFDLIFPIRDVSDNKKDEEIANYIIDLHRAYLNKDINRKIGLDYIEIDGVKVDREFIIKYIHYVRKHKPIIGERAKKMLINYYVEMRKKHQITARQLEAAIRIAEAHAKAKLKEEVDEEDAKVAIEIITECLKEIAYDPETGMLDVDKIIGVAKKERDKLMMVYEIIKELSDDDKLVDYDFILEEAKKRGINEEELENIIKKLIKYGDIDEPKPGRFRVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPY2</accession>
    <name>N6VPY2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">L-cysteine desulfidase</fullName>
            <ecNumber evidence="4">4.4.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">L-cysteine desulfhydrase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">J422_04915</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-cysteine + H2O = hydrogen sulfide + pyruvate + NH4(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24931"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="EC" id="4.4.1.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the L-cysteine desulfidase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.28" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95955.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592081.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPY2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00508"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.963"/>
    <dbReference type="OrthoDB" id="60297at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0080146">
        <property type="term" value="F:L-cysteine desulfhydrase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019450">
        <property type="term" value="P:L-cysteine catabolic process to pyruvate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005130">
        <property type="entry name" value="Ser_deHydtase-like_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021144">
        <property type="entry name" value="UPF0597"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30501">
        <property type="entry name" value="UPF0597 PROTEIN YHAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30501:SF2">
        <property type="entry name" value="UPF0597 PROTEIN YHAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03313">
        <property type="entry name" value="SDH_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Serine dehydratase-like alpha subunit" evidence="12">
        <location>
            <begin position="181"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011233"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00015549"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF03313"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN95955.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="365" mass="40992" checksum="F08159E5F352946D" modified="2013-06-26" version="1">MVIDEVLKNEIYKSLGCTEVALIGYTVAKAKPKDLKEIKEIKLILGKSVFKNAFSVGVPNTGKFGILPAVVGGLLGEKENGLEIFRDIEYDEELENIVRNRLKIEVVNKDVYCRVIINKIYEAESTYYYKRVDDKLKEKFKSLDLKDFLDYIDNLPKDIEELIKDVIKTNKEFVNGFLDIDFGDLNNIIKATASAVYNRMMGFNKEAYAIAGSGNMGLMATLPIIFYDRENRELIKSLALSALITIYATYHTSYISSMCGCVNRGGLGCVCGLAYYLNKDIEKAMKSFLANITGIICDGGKPSCSLKIASGVFSAYLSLFYETKDYEGIIGKDFKECIKNLSEITKSMNLEDKIIELMLKKDVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ26</accession>
    <name>N6VQ26_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable membrane transporter protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_05090</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cell membrane</location>
            <topology evidence="5">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 4-toluene sulfonate uptake permease (TSUP) (TC 2.A.102) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95990.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592152.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ26"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00471"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.998"/>
    <dbReference type="OrthoDB" id="60523at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002781">
        <property type="entry name" value="TM_pro_TauE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051598">
        <property type="entry name" value="TSUP/Inactive_protease-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN MJ0441-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43701:SF2">
        <property type="entry name" value="MEMBRANE TRANSPORTER PROTEIN YJNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01925">
        <property type="entry name" value="TauE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="47"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="133"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="172"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="199"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="237"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU363041"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95990.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="255" mass="27920" checksum="03E06316E67690AD" modified="2013-06-26" version="1">MNILFFITLIILGFFVGILGSLLGVSGGFIITPILTLIFESLSIPEAMKFAVGTSLFVVFINSIFALLRHRVNIRCGLYIGILGSLIAFIFGKLSILYIPSSVVKKIFGIFLIIISGYYLLLKDFYDDREVRFSHLVICGLISGILAGLFGIGGGIAIIPILSMFKYDPKKLVTVSISAIPIISFGGLISYLSANVDNYIYNIGYVSIPIAIIIAFPIAFSSKLGHKLFHKLDNNKIKILLSTILFIIGLVMLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQD3</accession>
    <name>N6VQD3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Group 1 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_04368</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96075.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591780.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQD3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00649"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.856"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="2">
        <location>
            <begin position="15"/>
            <end position="183"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="196"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96075.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="389" mass="44353" checksum="5F9C01525E5C4759" modified="2013-06-26" version="1">MKIAMVTWEYPPKIVGGLAIHCKGLAEALVKLGHEVDIITADHHLPEYENIRGVNVYRVRPLTHSHFLTYAILMAEEMEKKIGILKRDYDVVHCHDWMTHFVGANLKHVVNMPYVQSIHSTEQGRCGGIHSEDSRTIAGIEYLSTYEACQVITVSKSLKDEICYIYNVPEDKVRVIYNGINPEEFDIEISEEERLAIRRNIGVYDHEIMILYVGRLTYQKGVEYLIRAMPIILSKFPNAKLVIAGNGDMRGYLEDLSYRVGVRDRVIFLGFVGGDFLPKLYKASDIVVIPSIYEPFGIVALEAMAAGKPVVATSVGGLREIIKHEYNGIWVYPYNPESIAWGVERVLSDEGLRKYIINNAKKEVYEKYSWMNIAKETVKVYETAINMMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQJ8</accession>
    <name>N6VQJ8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Pyruvoyl-dependent arginine decarboxylase</fullName>
            <shortName evidence="6">PvlArgDC</shortName>
            <ecNumber evidence="6">4.1.1.19</ecNumber>
        </recommendedName>
        <component>
            <recommendedName>
                <fullName evidence="6">Pyruvoyl-dependent arginine decarboxylase subunit beta</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="6">Pyruvoyl-dependent arginine decarboxylase subunit alpha</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="6" type="primary">pdaD</name>
        <name evidence="7" type="ORF">J422_03848</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>L-arginine + H(+) = agmatine + CO2</text>
            <dbReference type="Rhea" id="RHEA:17641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="EC" id="4.1.1.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </cofactor>
        <text evidence="6">Binds 1 pyruvoyl group covalently per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the PdaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.19" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96160.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591368.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQJ8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00401"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.754"/>
    <dbReference type="OrthoDB" id="30748at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008792">
        <property type="term" value="F:arginine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006527">
        <property type="term" value="P:L-arginine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.60.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.20.10">
        <property type="entry name" value="Pyruvoyl-Dependent Histidine Decarboxylase, subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01404">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016104">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016105">
        <property type="entry name" value="Pyr-dep_his/arg-deCO2ase_sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002724">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00286">
        <property type="entry name" value="pyruvoyl-dependent arginine decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40438">
        <property type="entry name" value="PYRUVOYL-DEPENDENT ARGININE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40438:SF1">
        <property type="entry name" value="PYRUVOYL-DEPENDENT ARGININE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01862">
        <property type="entry name" value="PvlArgDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005216">
        <property type="entry name" value="Pyruvoyl-dep_arg_deCO2ase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00471">
        <property type="entry name" value="Pyruvoyl-dependent_arginine_de"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01170">
        <property type="entry name" value="Pyruvoyl-dependent_arginine_de"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00055">
        <property type="entry name" value="Pyruvoyl-Dependent_Histidine/A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56271">
        <property type="entry name" value="Pyruvoyl-dependent histidine and arginine decarboxylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3 6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 6" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="chain" id="PRO_5023277536" description="Pyruvoyl-dependent arginine decarboxylase subunit beta" evidence="6">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023277535" description="Pyruvoyl-dependent arginine decarboxylase subunit alpha" evidence="6">
        <location>
            <begin position="52"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage (non-hydrolytic)" evidence="6">
        <location>
            <begin position="51"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="modified residue" description="Pyruvic acid (Ser)" evidence="6">
        <location>
            <position position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96160.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="167" mass="18141" checksum="4853C3415E550EB2" modified="2013-06-26" version="1">MNIDIISPNLFKLPNTVSLVAGASEGKTELNAFDNALLKAGIGNVNLIRISSIMPPKAEIVPLPKLPMGALVPTAYGYIISKNPGEVIAAAVSVAIPKDDEKCGLIMEYEGVCSKKEAEERVREMAKIGFEERGWDIKRIESIAVEHEVERIGCAFAAAALWYKSKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQU8</accession>
    <name>N6VQU8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acetyl-CoA decarbonylase/synthase complex subunit beta</fullName>
            <shortName evidence="9">ACDS complex subunit beta</shortName>
            <ecNumber evidence="9">2.3.1.169</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">ACDS complex acyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">cdhC</name>
        <name evidence="11" type="ORF">J422_03191</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon complex generates CO from CO(2), while the beta subunit (this protein) combines the CO with CoA and a methyl group to form acetyl-CoA. The methyl group, which is incorporated into acetyl-CoA, is transferred to the beta subunit by a corrinoid iron-sulfur protein (the gamma-delta complex).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Co(I)-[corrinoid Fe-S protein] + acetyl-CoA + H(+) = methyl-Co(III)-[corrinoid Fe-S protein] + CO + CoA</text>
            <dbReference type="Rhea" id="RHEA:45212"/>
            <dbReference type="Rhea" id="RHEA-COMP:11110"/>
            <dbReference type="Rhea" id="RHEA-COMP:11111"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:85033"/>
            <dbReference type="ChEBI" id="CHEBI:85035"/>
            <dbReference type="EC" id="2.3.1.169"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </cofactor>
        <text evidence="9">Binds 1 [Ni-Fe-S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8 9">Monomer. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the CdhC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.169" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000019">
        <property type="protein sequence ID" value="ENN96275.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590889.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQU8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00315"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.626"/>
    <dbReference type="OrthoDB" id="69951at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016407">
        <property type="term" value="F:acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:anaerobic carbon-monoxide dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043884">
        <property type="term" value="F:CO-methylating acetyl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1650.10">
        <property type="entry name" value="Bifunctional carbon monoxide dehydrogenase/acetyl-coa synthase(codh/acs), Chain M, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1470.10">
        <property type="entry name" value="Bifunctional carbon monoxide dehydrogenase/acetyl-coa synthase(codh/acs), Chain M, domain 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.970.20">
        <property type="entry name" value="Carbon monoxide dehydrogenase alpha subunit. Chain D, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01138">
        <property type="entry name" value="CdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045822">
        <property type="entry name" value="ACS_CODH_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004461">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038571">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu_3_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023432">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00316">
        <property type="entry name" value="cdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003379">
        <property type="entry name" value="PRK04456.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42281">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42281:SF1">
        <property type="entry name" value="ACETYL-COA DECARBONYLASE_SYNTHASE COMPLEX SUBUNIT BETA 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19436">
        <property type="entry name" value="ACS_CODH_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03598">
        <property type="entry name" value="CdhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="Prismane protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0533">Nickel</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="CO dehydrogenase/acetyl-CoA synthase complex beta subunit C-terminal" evidence="10">
        <location>
            <begin position="165"/>
            <end position="394"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="277"/>
        </location>
        <ligand>
            <name>[Ni-Fe-S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60400"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF19436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96275.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="458" mass="51886" checksum="1D9B706B23138BCB" modified="2013-06-26" version="1">MFENIPVSVGPMNEGERIRKPDMYVELAGPKSYGFELVKVDEAEDKIEVIGKDLDEMEEGSINPFAIIIKVKGKKIEEDLEGVLERRVHEFLNYIEGVMHLNQRDQIWIRISKDAYKKGLRLKHIAEVVQKLFKAEFPFIEKCDVIIITDPDKVKEELEKAREIYNKRDERIKSLRDEDVDVFYGCIMCQSFAPTHVCVISPERPALCGGINYLDARAAAKIDPNGPIFEIKKGKCLDEKLGVYEGVNEVVKEKSQGAIERVSLYSALVDPCTSCGCFEAVVFYIPEVDGFGIAHRAFKGETPLGIPFSTLAGQCSGGKQVPGFVGVSIAYMKSRKFLQGDGGWERVVWLPKELKERLKEDIPKDLFDKIATDEITNVEELKKFLKEKDHPVVKRWVEESEVVEVEEETEEEKPDLEAPVINLPGEFLGLPSGIRIELYNAIIKAEKIVIYKENGKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR00</accession>
    <name>N6VR00_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Carbamoyl phosphate-converting enzyme HypF</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">[NiFe]-hydrogenase maturation factor HypF</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_03184</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>C-terminal L-cysteinyl-[HypE protein] + carbamoyl phosphate + ATP + H2O = C-terminal S-carboxamide-L-cysteinyl-[HypE protein] + AMP + phosphate + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55636"/>
            <dbReference type="Rhea" id="RHEA-COMP:14247"/>
            <dbReference type="Rhea" id="RHEA-COMP:14392"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:76913"/>
            <dbReference type="ChEBI" id="CHEBI:139126"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Protein modification; [NiFe] hydrogenase maturation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the carbamoyltransferase HypF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96330.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590801.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR00"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00183"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.625"/>
    <dbReference type="OrthoDB" id="371970at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016743">
        <property type="term" value="F:carboxyl- or carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.40:FF:000124">
        <property type="entry name" value="Carbamoyltransferase HypF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051060">
        <property type="entry name" value="Carbamoyltrans_HypF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004421">
        <property type="entry name" value="Carbamoyltransferase_HypF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041440">
        <property type="entry name" value="HypF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055128">
        <property type="entry name" value="HypF_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00143">
        <property type="entry name" value="hypF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42959">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42959:SF1">
        <property type="entry name" value="CARBAMOYLTRANSFERASE HYPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17788">
        <property type="entry name" value="HypF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22521">
        <property type="entry name" value="HypF_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <keyword evidence="5" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="YrdC-like" evidence="10">
        <location>
            <begin position="15"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00075001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00078219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96330.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="569" mass="65145" checksum="141DAD72F5F460E2" modified="2013-06-26" version="1" fragment="single">HVYLTDGHETLYERDEAIRETVKLLEEGYIVAVKGIGGCHLCCITEDEPVLTLRERLNRKSQPFAIMSMLENVEKFAYISKLEREVLISNRRPIVVLKKRKGYDKYISKYVSNLDTIGVMLPYSGLHYLLFNKAIAYVFTSANLPGLPMVKDNEDILNKMNGIADYFLLHNRRIVNRCDDSVVKKVADRIVFLRRSRGYAPEPVKVKLDNNKTILCLGAELNSCVCLAKRDNFYLSQYIGNTAKYETFLYLKEAIFRLLELTNTKKVDVIACDLHPTYNSTKLAQELQEIFDCDLIRVQHHFAHAYSLLGDNNYFEDAIILALDGMGYGLDGNVWGGEVLLYRDGEIKRVSHLEEQYQLGGDLAVKYPARMLFSILYKAIKEEALEFMSKYFPENMLRILEFQLKKKINCPITTSTGRVLDAVSALLEICLEKTYDGEPAIRLEAEVKKDDVEVEPRIKNNILITTDMIKQCYEMLINGESKGKIAYYAHLYIADGLFEIFKKLSEKYGINTIGLTGGVSYNRVITERIYERANEEGYNFIYHRNVPNGDGGVSFGQAIYYIKERGKNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR04</accession>
    <name>N6VR04_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0056 membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_07212</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000080">
        <property type="protein sequence ID" value="ENN95556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004595246.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000080.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR04"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00557"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1392"/>
    <dbReference type="OrthoDB" id="10856at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00427">
        <property type="entry name" value="NAAT family transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508:SF1">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="54"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="86"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="118"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="8">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="146" mass="15821" checksum="663572E38F90D540" modified="2013-06-26" version="1" fragment="single">FGEYILKFFGITLNAFKIAGGLLLVLIALDMVRGHQKSKIHEKDIKHAEDVDEIALMPLATPLLAGPGSITACMVAMAGAEGFNKFLVLLAIGLCIIITYITLLFAEKIIEKIGKLGIRIFTRMMGFLLLAIAVQMIISGLKGSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR09</accession>
    <name>N6VR09_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Exosome subunit</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02629</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96340.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590559.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR09"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00078"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.515"/>
    <dbReference type="OrthoDB" id="10874at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002739">
        <property type="entry name" value="PAB1135-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38816:SF1">
        <property type="entry name" value="EXOSOME SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38816">
        <property type="entry name" value="EXOSOME SUBUNIT, DUF54 FAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01877">
        <property type="entry name" value="RNA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96340.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="17630" checksum="3C152D5A87AC4751" modified="2013-06-26" version="1">MINSIKISSIAHATEDLEKVLKAIEFLIPEDVDDEKIDIEVVETEGYFRNPIRIINLNAEGKEAEKIYKHIIELIKSNEKNINKLRRDLHLRIEDNKFYIRFDKQKAFLGEARVVDGDDVIRVVINFKIYTKNKEDIVKELLTKELGICMS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR11</accession>
    <name>N6VR11_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Protein translocase subunit SecE</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Protein transport protein Sec61 gamma subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">secE</name>
        <name evidence="9" type="ORF">J422_02654</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Component of the Sec protein translocase complex. Heterotrimer consisting of SecY (alpha), SecG (beta) and SecE (gamma) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="7">Endomembrane system</location>
            <topology evidence="7">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the SecE/SEC61-gamma family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96345.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590569.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR11"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00083"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.520"/>
    <dbReference type="OrthoDB" id="52835at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008320">
        <property type="term" value="F:protein transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009306">
        <property type="term" value="P:protein secretion"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.820">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00422">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023391">
        <property type="entry name" value="Prot_translocase_SecE_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008158">
        <property type="entry name" value="Translocase_Sec61-g"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001901">
        <property type="entry name" value="Translocase_SecE/Sec61-g"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006907">
        <property type="entry name" value="PRK09400.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00327">
        <property type="entry name" value="secE_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00584">
        <property type="entry name" value="SecE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103456">
        <property type="entry name" value="Preprotein translocase SecE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3 8" id="KW-0653">Protein transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0811">Translocation</keyword>
    <keyword evidence="2 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="1 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="38"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96345.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="67" mass="7852" checksum="9DE8C25C3FE23F49" modified="2013-06-26" version="1">MDFDRKIEKLKEFLEECRRVWLVLKKPTKEEYINVAKITALGITVLGVIGYIIHVPAVYIKTTIFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR44</accession>
    <name>N6VR44_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cobalt-precorrin-6Y C(5)-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_02859</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96380.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590640.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR44"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00121"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.561"/>
    <dbReference type="OrthoDB" id="42238at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11644">
        <property type="entry name" value="Precorrin-6Y-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012818">
        <property type="entry name" value="CbiE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050714">
        <property type="entry name" value="Cobalamin_biosynth_MTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02467">
        <property type="entry name" value="CbiE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004460">
        <property type="entry name" value="PRK05787.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182">
        <property type="entry name" value="COBALT-PRECORRIN-6B C(15)-METHYLTRANSFERASE (DECARBOXYLATING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-7 C(5)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96380.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="204" mass="23265" checksum="48786CD39C0A5533" modified="2013-06-26" version="1">MIYLIGIGPGDKEFITLKALKIIKKADVLIGSKRALSIFEHNNKVILGKNIKDDLKNAIERYKNKEIAILSTGDPCFSGLLKTLKSIGINNITIIPGISSIQIAAAKLKISWEDYHILTLHGKPKNRDKLLKYLKNNENVIFLPNNLKDDIKYLISNGVNEELEIYILENLTYENERIRKMKLKDILNDEFSYLTVCVYKGEDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR88</accession>
    <name>N6VR88_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF192 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06613</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000056">
        <property type="protein sequence ID" value="ENN95661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593800.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR88"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00362"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1287"/>
    <dbReference type="OrthoDB" id="64208at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1140">
        <property type="entry name" value="Protein of unknown function DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003795">
        <property type="entry name" value="DUF192"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038695">
        <property type="entry name" value="Saro_0823-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37953">
        <property type="entry name" value="UPF0127 PROTEIN MJ1496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37953:SF1">
        <property type="entry name" value="UPF0127 PROTEIN MJ1496"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02643">
        <property type="entry name" value="DUF192"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="108" mass="13026" checksum="AA44A3486FB6A088" modified="2013-06-26" version="1">MKKIKIGELEFEVIIADNIIKRALGLMLRDIKDKAMLFLYKRRKVSIHTFFMFYPIDVVFIDNYRVVDAVRLNPWSYYKSKEYSTAMLEYKCREIEPKKLIGEIVIFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRB1</accession>
    <name>N6VRB1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Biotin synthase</fullName>
            <ecNumber evidence="9">2.8.1.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_02085</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.6" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96440.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590217.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRB1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01166"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.410"/>
    <dbReference type="OrthoDB" id="61910at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023858">
        <property type="entry name" value="HcgA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03957">
        <property type="entry name" value="rSAM_HmdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="BIOTIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976:SF2">
        <property type="entry name" value="BIOTIN SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01060">
        <property type="entry name" value="BATS_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00330">
        <property type="entry name" value="HMD_cofactor_maturase_(HmdB-li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01279">
        <property type="entry name" value="HMD_cofactor_maturase_(HmdB-li"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="55"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96440.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="351" mass="39822" checksum="D8BE0658700726DB" modified="2013-06-26" version="1">MVFDYIREEIEELKEGKVFSLIDEDMALKLFKLDNWRDFLELFKLASEVRDIFKKEIEITSTIHVTNICHVNPKCLYCGFAAGTSKEGYYNPFRLTDEEIKEAAIAIEESGIRRVSCSTAHGYGGKEALRALKIVKKYTSLEVLVNAGADLTEEVIKEMKKYDINTICCNLETINEKLFKKLKPGEELEDRIRVCKLVNKYGIELSSGLLIGVGESYEDRVKHLFYLKNELEVGEIPIMGFNPYKGTPMENFPRCSALEQAKTIAITRLIFPNIRITSPSPTIGPELIYFSLLAGASNIATVIPKNYPRIVKGVGNPKVANLDDTVKIIESLGLKPKLDYNKYYMYLKSQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRB7</accession>
    <name>N6VRB7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coenzyme F420 hydrogenase/dehydrogenase subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_06421</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95701.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593535.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRB7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01290"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1250"/>
    <dbReference type="OrthoDB" id="38261at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052592">
        <property type="term" value="F:oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007516">
        <property type="entry name" value="Co_F420_Hydgase/DH_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045220">
        <property type="entry name" value="FRHB/FDHB/HCAR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007525">
        <property type="entry name" value="FrhB_FdhB_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332">
        <property type="entry name" value="7-HYDROXYMETHYL CHLOROPHYLL A REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31332:SF0">
        <property type="entry name" value="7-HYDROXYMETHYL CHLOROPHYLL A REDUCTASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04432">
        <property type="entry name" value="FrhB_FdhB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04422">
        <property type="entry name" value="FrhB_FdhB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="8"/>
            <end position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95701.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="354" mass="39665" checksum="1F54B28FEC5C4880" modified="2013-06-26" version="1">MKSYKDLKAEVWDKNRCSGCGACVAVCPVNNLYFKEESPVKFICDECSCMIVPAKTEEHPLSAEFCKTVNYDVPCGACYDACPRIRELAINDPLGKYLKILKAKASTEIKYAQNGGVVTAILANAFEEELIDGAIVMKEDRWTLEPQSFLAMSKEDVLKSAGSKYLRKGNVLQAFKKAVMEEKLKKLAIVGTPCVISAICNILSSDNDLLGPFRRAIRLKISLFCFEIFDYNKMIDLLEKEGINPWDIKKMDILTGKLRITFVSGEYKDYNLKELEFAVREGCKCCGDFTGVCSDISVGNIGSGEGYSTVIIRNNWGEGFFLRAAHNGYLSYDDKVNVEEIVKLARLKMKRIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRC8</accession>
    <name>N6VRC8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">H/ACA RNA-protein complex component Gar1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06481</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593555.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRC8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01279"/>
    <dbReference type="OrthoDB" id="60264at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.230">
        <property type="entry name" value="Probable tRNA pseudouridine synthase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038664">
        <property type="entry name" value="Gar1/Naf1_Cbf5-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007504">
        <property type="entry name" value="H/ACA_rnp_Gar1/Naf1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04410">
        <property type="entry name" value="Gar1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="68" mass="7769" checksum="857F1054D726C5F1" modified="2013-06-26" version="1">MKIKILHKTPKGFLIGRGKNVKINSPVYFKKKKIGVIVDIFGPVSNPYIKIKPIKKDINVDFVFTNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRD4</accession>
    <name>N6VRD4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Tetrahydromethanopterin S-methyltransferase subunit A</fullName>
            <ecNumber evidence="11 12">7.2.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mtrA</name>
        <name evidence="13" type="ORF">J422_02160</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="7.2.1.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>5-hydroxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:60494"/>
        </cofactor>
        <text evidence="11">Binds 1 5-hydroxybenzimidazolylcobamide group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the MtrA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.1.4" evidence="11 12"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96455.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590236.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRD4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01180"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.424"/>
    <dbReference type="OrthoDB" id="130682at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01093">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030688">
        <property type="entry name" value="MeTrfase_MtrA/MtxA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005778">
        <property type="entry name" value="MtrA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01111">
        <property type="entry name" value="mtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002126">
        <property type="entry name" value="PRK00964.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04208">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500207">
        <property type="entry name" value="MtrA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009452">
        <property type="entry name" value="MtrA_MtxA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 11" id="KW-0170">Cobalt</keyword>
    <keyword evidence="9 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 11" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3 11 13" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 13" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="221"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>5-hydroxybenzimidazolylcob(I)amide</name>
            <dbReference type="ChEBI" id="CHEBI:60494"/>
            <note>cofactor</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR01111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96455.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="240" mass="25847" checksum="DEC8034D5CAD263B" modified="2013-06-26" version="1">MANKKEPAPGWPIVSGEYVVGDPESCVGVVTLGSHGLEKACVEAGAAIAGPCHTENLGIEKVVANYISNPNIRFMILCGSEVQGHLTGQCFKALWENGIDETGKIVGAKGAIPFLENVGKEAVERFRRQIIEVIDLIDCEDINKITQAIKECISKDPGAIDEEPMILELEGGAKGEEGEGELRPLSAEIALIEARMRMIYDKINATALLNKYNSGYYNGKIQGIAIGLFLSLLIFSLLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRJ8</accession>
    <name>N6VRJ8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01760</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96505.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590110.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRJ8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00203"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.346"/>
    <dbReference type="OrthoDB" id="145002at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053281">
        <property type="entry name" value="Double_zinc_ribbon"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025874">
        <property type="entry name" value="DZR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026870">
        <property type="entry name" value="Zinc_ribbon_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36718:SF1">
        <property type="entry name" value="DOUBLE ZINC RIBBON PROTEIN MJ0416"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36718">
        <property type="entry name" value="OS05G0435400 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12773">
        <property type="entry name" value="DZR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13240">
        <property type="entry name" value="Zn_Ribbon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Zinc-ribbon" evidence="2">
        <location>
            <begin position="101"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="domain" description="DZANK-type" evidence="1">
        <location>
            <begin position="133"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13240"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96505.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="190" mass="22000" checksum="7C0CCAAD3CB15B29" modified="2013-06-26" version="1">MGKVVPLTDEEKMSIVSGLRSSVPATRLVTLRRLQDLASRKPETILYLDAYDKVTLNEILTLLNHIAEYDPDEILRREAVITMEAVKKALGFKFSEVIPTCTNCGNPIDVGWDYCVNCGAEIDKMELEDIKRCPNCNKYIFETWKFCAHCKYKLREEEEVITRCPRCKRPVDPEWLVCPYCGYRLKRVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRK1</accession>
    <name>N6VRK1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA (guanine(37)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.228</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">M1G-methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA [GM37] methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_05988</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>guanosine(37) in tRNA + S-adenosyl-L-methionine = N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36899"/>
            <dbReference type="Rhea" id="RHEA-COMP:10145"/>
            <dbReference type="Rhea" id="RHEA-COMP:10147"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="EC" id="2.1.1.228"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.228" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593053.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRK1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00561"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1166"/>
    <dbReference type="OrthoDB" id="8079at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052906">
        <property type="term" value="F:tRNA (guanine(37)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002939">
        <property type="term" value="P:tRNA N1-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.110:FF:000001">
        <property type="entry name" value="tRNA (guanine(37)-N1)-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2580">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.110">
        <property type="entry name" value="Met-10+ protein-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040601">
        <property type="entry name" value="Trm5a/b_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047732">
        <property type="entry name" value="tRNAMtaseTrm5bMeth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF36">
        <property type="entry name" value="TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18093">
        <property type="entry name" value="Trm5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="10">
        <location>
            <begin position="94"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00029736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="332" mass="38870" checksum="A2EBD19BD4086B6C" modified="2013-06-26" version="1">MLTLKIDKKLGEKVRRLLIENDLYDKDYLIDRDDKHLYLPIKHVDEKILREILKVDFEIVERDLKKRKKEKSFRDVINEKYRDIVDKKLLALSYDVIGDLVILQISDEVREDIRKEIGELAYKLIPCKGVFRRKSEVKGEFRIREIEHLAGENRTLTIHKENNYRLYVDIAKVYFSPRLSGERKRIGESVGLNETVIDMFAGVGPFSIACRRARRVYAIDINPEAISLLKKNIELNKVKHKIIPILADANEVDLKGDRVIMNLPKYSYKFVDKALSMINKNGIIHYYTIGENFSEGIKVFEGKCNFEILNKKIVKSYSPKKYIMAFDIIVKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRK8</accession>
    <name>N6VRK8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">UDP-N-acetylglucosamine 2-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01830</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96515.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590121.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRK8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00215"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.360"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd03786">
        <property type="entry name" value="GTB_UDP-GlcNAc_2-Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003331">
        <property type="entry name" value="UDP_GlcNAc_Epimerase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029767">
        <property type="entry name" value="WecB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00236">
        <property type="entry name" value="wecB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43174">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE 2-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43174:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE 2-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02350">
        <property type="entry name" value="Epimerase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDP-N-acetylglucosamine 2-epimerase" evidence="1">
        <location>
            <begin position="26"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96515.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="360" mass="41103" checksum="50167E0FB69C25BE" modified="2013-06-26" version="1">MISIILGTRPEIIKLSPIIRELKSEEFFIIHTNQHYSENMDKIFFQELNLPKPKYNLNIGSGTHGEQTGKMLIEIEKVLLKEKPNIVIVQGDTNTVLAGALAASKLKIKVAHVEAGLRSYDKNMPEEINRVLTDHISDYLFAPTEIAKNNLLKEGIKDNIFVVGNTIVDATLQNLEIAEKNDHIKGFFNSVVDKDYFLLTLHRAENVDNRERLERIVNNINELSDIYDYDIIFPIHPRTKKRLIEFNLFDKLKSNKKIKIIEPVGYLEFLILEKNAKLILTDSGGVQEEACILKIPCITLRDNTERPETLEVGSNILVGDNKEKLIKAVELMLNKKRDWKNPFGDGKAGKRIVRILRGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRR4</accession>
    <name>N6VRR4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Anhydromevalonate phosphate decarboxylase</fullName>
            <ecNumber evidence="12">4.1.1.126</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">J422_05643</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the conversion of trans-anhydromevalonate 5-phosphate (tAHMP) into isopentenyl phosphate. Involved in the archaeal mevalonate (MVA) pathway, which provides fundamental precursors for isoprenoid biosynthesis, such as isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2E)-3-methyl-5-phosphooxypent-2-enoate + H(+) = isopentenyl phosphate + CO2</text>
            <dbReference type="Rhea" id="RHEA:78971"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:229665"/>
            <dbReference type="EC" id="4.1.1.126"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:78972"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>prenylated FMN</name>
            <dbReference type="ChEBI" id="CHEBI:87746"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the UbiD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.126" evidence="12"/>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95851.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592698.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRR4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00265"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1101"/>
    <dbReference type="OrthoDB" id="8480at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016831">
        <property type="term" value="F:carboxy-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1670.10:FF:000003">
        <property type="entry name" value="Phenolic acid decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1670.10">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002830">
        <property type="entry name" value="UbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049381">
        <property type="entry name" value="UbiD-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048304">
        <property type="entry name" value="UbiD_Rift_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00148">
        <property type="entry name" value="UbiD family decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108">
        <property type="entry name" value="3-OCTAPRENYL-4-HYDROXYBENZOATE CARBOXY-LYASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30108:SF21">
        <property type="entry name" value="4-HYDROXYBENZOATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01977">
        <property type="entry name" value="UbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20696">
        <property type="entry name" value="UbiD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143968">
        <property type="entry name" value="UbiD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="5" id="KW-0288">FMN</keyword>
    <keyword evidence="8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like Rift-related" evidence="15">
        <location>
            <begin position="89"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="3-octaprenyl-4-hydroxybenzoate carboxy-lyase-like C-terminal" evidence="16">
        <location>
            <begin position="271"/>
            <end position="391"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF01977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF20696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN95851.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="405" mass="46138" checksum="15124B6B3DA42561" modified="2013-06-26" version="1">MREFINLLNPITINKADKRYEVAKVLKKYDGKPVYIKDVDGYEILGNLWSRDSLSKFFNVEKEKFLFFMLKKLDDLKEPKIVKFSGYKKESPDFIHSLPIPIYYEKDPGPYITSGVVVAYDEDYGYNMSIHRMLVKEDHLVIRMVEQRHLYHYYHKAIKEKGYLDVYITIGNHPAVLLAASTSGDITFDELKFASSLSDVDVFSLDFLVPKAEIIIKAKILNELDDEGPFVDITGTYDKVRKQPIVEIEKIYVKDNFIFHALLPGGIEHKTLMGLPQEPRMFKYIRNTIPSVKNVRLTEGGCCWLHAIVQIEKKTEGDGKNAILSALASHPSLKHVVVVDDDINIFDINDIEYAIATRVQGDRDIVIIKGAKGSSLDPSSDGLTTKIGIDATKPLNCKEKFERII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRW7</accession>
    <name>N6VRW7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA(Ile2) 2-agmatinylcytidine synthetase TiaS</fullName>
            <shortName evidence="6">tRNA(Ile2)-agm2C synthetase</shortName>
            <ecNumber evidence="6">6.3.4.22</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">tRNA(Ile2) agmatidine synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">tiaS</name>
        <name evidence="11" type="ORF">J422_01378</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">ATP-dependent agmatine transferase that catalyzes the formation of 2-agmatinylcytidine (agm2C) at the wobble position (C34) of tRNA(Ile2), converting the codon specificity from AUG to AUA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>cytidine(34) in tRNA(Ile2) + agmatine + ATP + H2O = 2-agmatinylcytidine(34) in tRNA(Ile2) + AMP + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43608"/>
            <dbReference type="Rhea" id="RHEA-COMP:10625"/>
            <dbReference type="Rhea" id="RHEA-COMP:10626"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58145"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="ChEBI" id="CHEBI:83545"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.22"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TiaS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.22" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589939.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRW7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00013"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.270"/>
    <dbReference type="OrthoDB" id="39189at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002101">
        <property type="term" value="P:tRNA wobble cytosine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04482">
        <property type="entry name" value="RPA2_OBF_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1010">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01892">
        <property type="entry name" value="tRNA_Ile2_agm2C_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053870">
        <property type="entry name" value="TiaS-like_TCKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013696">
        <property type="entry name" value="TiaS_FLD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024913">
        <property type="entry name" value="tRNA_Ile2__agm2C_synt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055394">
        <property type="entry name" value="Zn_ribbon_TiaS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705">
        <property type="entry name" value="TRNA(ILE2) 2-AGMATINYLCYTIDINE SYNTHETASE TIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705:SF1">
        <property type="entry name" value="TRNA(ILE2) 2-AGMATINYLCYTIDINE SYNTHETASE TIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08489">
        <property type="entry name" value="TiaS_FLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22641">
        <property type="entry name" value="TiaS_TCKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23783">
        <property type="entry name" value="Zn_ribbon_TiaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TiaS-like TCKD" evidence="9">
        <location>
            <begin position="2"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="TiaS FLD" evidence="8">
        <location>
            <begin position="142"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="7">
        <location>
            <begin position="268"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="domain" description="TiaS C-terminal zinc ribbon" evidence="10">
        <location>
            <begin position="352"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF22641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF23783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="420" mass="48790" checksum="FD15681A4C2F0275" modified="2013-06-26" version="1">MFIGIDDTDSRERYCTTYIATLLMEELSNYKLDDPRLIRFNPMVKYKTRGNGGVAIRILDKVDERDKEEIKDITLKIIEKYSDMDNDNTNPGVVFLDEENYRNNINLLRDYYKKTLFDILDINFAEDILKKINADYYKFKLGRGIIGALGAIAFFGDYTYELIAYRKKENWGKKRRIDKESVIIMDKETFPYTYDNYDYENDKILIAPNTPCPVLFGIRGIDKDILIKAMEMIKGEEPERFMIFKTNHGTDAHLRRMKIKDIYPNTGVIVYGKVVNNPKDIEGGHVIFKISDGTGEISCAAYEPTKGFREIIRKLIVGDYVAVYGTVREEPLTINLEKIKILRLEKKYILDKRCPFCGGTLKAKGKKVGFKCRKCKKVIKYDKIKKIEVERDLKIGFYEVPGSARRHLSKPIQLIDLVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS34</accession>
    <name>N6VS34_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit Rpo4</fullName>
            <ecNumber evidence="1">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">DNA-directed RNA polymerase subunit F</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">rpo4</name>
        <name evidence="1" type="synonym">rpoF</name>
        <name evidence="2" type="ORF">J422_00926</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. This subunit is less well bound than the others.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Part of the RNA polymerase complex. Forms a stalk with Rpo7 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic RPB4 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589855.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS34"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00589"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.182"/>
    <dbReference type="OrthoDB" id="25158at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.80">
        <property type="entry name" value="HRDC domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00864">
        <property type="entry name" value="RNApol_arch_Rpo4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010997">
        <property type="entry name" value="HRDC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044876">
        <property type="entry name" value="HRDC_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005574">
        <property type="entry name" value="Rpb4/RPC9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010924">
        <property type="entry name" value="Rpo4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011554">
        <property type="entry name" value="PRK14981.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39646:SF1">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT RPO4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39646">
        <property type="entry name" value="RNA POLYMERASE RPB4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03874">
        <property type="entry name" value="RNA_pol_Rpb4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005053">
        <property type="entry name" value="RNA_pol_F_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47819">
        <property type="entry name" value="HRDC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="1 2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="107" mass="12421" checksum="B8795218543252C9" modified="2013-06-26" version="1">MIGKKILSERYITISEALEIMEDRAKIGELSYEQGCALDYLQKFAKLKKEDAIELVKELKNLGLDEKDAVKIADILPKDMDDLRAIFYKRELPENAEEILEVVRKYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS97</accession>
    <name>N6VS97_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cobyrinic acid ac-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_00496</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589781.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS97"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00051"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.95"/>
    <dbReference type="OrthoDB" id="31168at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02034">
        <property type="entry name" value="CooC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001573">
        <property type="entry name" value="Carbon monoxide dehydrogenase accessory protein CooC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014433">
        <property type="entry name" value="CooC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF7">
        <property type="entry name" value="CARBON-MONOXIDE DEHYDROGENASE ACCESSORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005647">
        <property type="entry name" value="CooC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="3">
        <location>
            <begin position="2"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="248" mass="28199" checksum="4B11731556ECB6B6" modified="2013-06-26" version="1">MIIAVSGKGGVGKTAFTTLLIKALSKKTNSILVVDADPDSNLPETLGVEVEKTVGDIREELKKAIEENKDIGMSKIDYLKSKVYEILVETDNYDLLVMGRPEGSGCYCSVNNWLRQIIDELSKYYEYVVIDTEAGLEHLSRRTTQNVDTMIVITDPSKRGLGTAKRIKKLANELEVKFKDIYVVANKVKPEHEKLIEEYAKELKLNLIGKLPYNKEIAEYDLKGIPLWYLPEDNEVYKKVKEIVDKYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSE6</accession>
    <name>N6VSE6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Helix-turn-helix type 11 domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00065</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589706.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSE6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01154"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.13"/>
    <dbReference type="OrthoDB" id="63512at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013196">
        <property type="entry name" value="HTH_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08279">
        <property type="entry name" value="HTH_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helix-turn-helix type 11" evidence="1">
        <location>
            <begin position="71"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08279"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="132" mass="15780" checksum="091889D46DE85164" modified="2013-06-26" version="1">MNIKEEIEKLVEKKEYDFWTFLKKAYEKDIKLDIGHFILLNLLIGVKDIYKKLINEIGEEEARKILEKNRIFTKNSDYVSGEFLKNYINRKSRVAVHNRIKDLKTLGFKIESKSGPFGGYKLIEYPEWFKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSG8</accession>
    <name>N6VSG8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific)</fullName>
            <ecNumber evidence="4">2.5.1.89</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Undecaprenyl diphosphate synthase</fullName>
            <shortName evidence="4">UDS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Undecaprenyl pyrophosphate synthase</fullName>
            <shortName evidence="4">UPP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">uppS</name>
        <name evidence="5" type="ORF">J422_04493</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with geranylgeranyl diphosphate (GGPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-undecaprenyl diphosphate (tritrans,heptacis-UPP). It is probably the precursor of glycosyl carrier lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>geranylgeranyl diphosphate + 7 isopentenyl diphosphate = tri-trans,hepta-cis-undecaprenyl diphosphate + 7 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:27622"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57533"/>
            <dbReference type="ChEBI" id="CHEBI:60388"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="EC" id="2.5.1.89"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="4">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the UPP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.89" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96096.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591825.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSG8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00626"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.881"/>
    <dbReference type="OrthoDB" id="8293at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045547">
        <property type="term" value="F:ditrans,polycis-polyprenyl diphosphate synthase [(2E,6E)-farnesyl diphosphate specific] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016094">
        <property type="term" value="P:polyprenol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00475">
        <property type="entry name" value="Cis_IPPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1180.10:FF:000003">
        <property type="entry name" value="Isoprenyl transferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1180.10">
        <property type="entry name" value="Decaprenyl diphosphate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01139">
        <property type="entry name" value="ISPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001441">
        <property type="entry name" value="UPP_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018520">
        <property type="entry name" value="UPP_synth-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036424">
        <property type="entry name" value="UPP_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00055">
        <property type="entry name" value="uppS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291:SF43">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE COMPLEX SUBUNIT DHDDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10291">
        <property type="entry name" value="DEHYDRODOLICHYL DIPHOSPHATE SYNTHASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01255">
        <property type="entry name" value="Prenyltransf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64005">
        <property type="entry name" value="Undecaprenyl diphosphate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01066">
        <property type="entry name" value="UPP_SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="4">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="4">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="48"/>
            <end position="51"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="92"/>
            <end position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="225"/>
            <end position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="238"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96096.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="270" mass="32130" checksum="D2454529DC474D6D" modified="2013-06-26" version="1">MKKFLKKIFLKTLNALENYGILKIYEKILEMQIDKNNLPRHVGIIMDGNRRTAEIFGKERYYGHYLGAKKVKEVLKWAKDLGIKIVTLYAFSLENFKRPKEEIEKLMELFENKFYEIADDEEIHKNKVRILAIGRRHLLPENVRKAIDYAESKTKNYNNFYVVVAIAYGGQQEIIDAVKKIAYKVKNGEINPEDIDKDLIDKHLYTANLPFPNPDLIIRTSGEERISNFLIWQSSYSELYFCDIYWPLFRKVDFLRAIREYQKRERRFGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSJ9</accession>
    <name>N6VSJ9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Small ribosomal subunit protein eS8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps8e</name>
        <name evidence="8" type="ORF">J422_03743</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the eukaryotic ribosomal protein eS8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96141.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591332.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSJ9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00381"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.731"/>
    <dbReference type="OrthoDB" id="372305at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11382">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.10.310:FF:000002">
        <property type="entry name" value="30S ribosomal protein S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00029">
        <property type="entry name" value="Ribosomal_eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001047">
        <property type="entry name" value="Ribosomal_eS8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018283">
        <property type="entry name" value="Ribosomal_eS8_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020919">
        <property type="entry name" value="Ribosomal_protein_eS8_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022309">
        <property type="entry name" value="Ribosomal_Se8/biogenesis_NSA2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00307">
        <property type="entry name" value="eS8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10394">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01201">
        <property type="entry name" value="Ribosomal_S8e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01193">
        <property type="entry name" value="RIBOSOMAL_S8E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 6 8" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00035277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96141.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="130" mass="14671" checksum="65DC5B000ABD33F5" modified="2013-06-26" version="1">MSVWQGRSRRKPSGGMYKKARKKRKYEMGREPIETHVCSEGMKLKIVRVRGGNRKVKVVRTAYANVIDPETNIAKKVKIITVKENQANIHYVRRNVITKGAIIETELGLAKVTSRPGQDGTVNAVLIKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSK9</accession>
    <name>N6VSK9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Transcription termination factor FttA</fullName>
            <ecNumber evidence="12">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">fttA</name>
        <name evidence="17" type="ORF">J422_03828</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Terminates transcription on the whole genome. Termination is linked to FttA-mediated RNA cleavage and does not require NTP hydrolysis. Cleaves endonucleolytically at the RNA exit channel of RNA polymerase (RNAP); the 5'-3' exonuclease activity of this protein degrades the nascent RNA released from RNAP.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="12">Binds 2 Zn(2+) ions, which are required for nuclease activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer. Interacts with RNA polymerase (RNAP), interacts with the Spt4-Spt5 complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. FttA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="12"/>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96156.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591360.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSK9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00397"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.750"/>
    <dbReference type="OrthoDB" id="7155at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004532">
        <property type="term" value="F:RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22532">
        <property type="entry name" value="KH-II_CPSF_arch_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02410">
        <property type="entry name" value="KH-II_CPSF_arch_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16295">
        <property type="entry name" value="TTHA0252-CPSF-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00870">
        <property type="entry name" value="FttA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019975">
        <property type="entry name" value="aCPSF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033769">
        <property type="entry name" value="TffA_KH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03675">
        <property type="entry name" value="arCOG00543"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF51">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17214">
        <property type="entry name" value="KH_TffA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 12" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 12 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="11" id="KW-0804">Transcription</keyword>
    <keyword evidence="9 12" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 12" id="KW-0806">Transcription termination</keyword>
    <keyword evidence="6 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="K Homology" evidence="14">
        <location>
            <begin position="95"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="15">
        <location>
            <begin position="192"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="16">
        <location>
            <begin position="419"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="region of interest" description="KHb" evidence="12">
        <location>
            <begin position="70"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="region of interest" description="Beta-Casp" evidence="12">
        <location>
            <begin position="382"/>
            <end position="575"/>
        </location>
    </feature>
    <feature type="region of interest" description="Metallo-beta-lactamase C-terminus" evidence="12">
        <location>
            <begin position="576"/>
            <end position="634"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="244"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="601"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN96156.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="634" mass="71560" checksum="B2F46A56783088BE" modified="2013-06-26" version="1">MSAEEILENLKKEIIKRSPKEAKIIDVQFEGPELVIYVKNPEVFTNEIVKKLAKDLRKRISVRPDPSVLLEPELAKKKILEIVPEDAGIVNCVFDPNTGEVIIESKKPGLVIGKEGKTLEEIKKAIKWAPKPVRVPPIQSETIKAIRATLYRERDEVKEILRRIGRRIHRDVVIKGDYWIRVSFLGGAREVGRSCLLVQTPDTRVLLDCGINVAYDDKTFPHFEAPEFSIEDLDAVIITHAHLDHCGFVPGLFRYGYDGPVYCTRPTRDLMTLLQKDYLDIAMKEGKPLPYSAKDIKTCVKHTIPIDYGVTTDISPTIKLTLHNAGHVLGSAIAHLHFGEGLYNLAYTGDIKFEPSRLLEPAVCQFPRLETLIIESTYGGYDDVLPSREEAERELLKVVSETIDKNGKVLIPVFGVGRAQELMLVLEEGYNQGIFNAPVYLDGMIWEATAIHTAYPEYLSKEIRQKIFHEGDNPFLSDVFKRVGSTNERRNIIDSDEPCIILATSGMLTGGPSLEYFKNLAPDEKNAIIFVGYQAEGTLGRKIQRGWKEIPIVTRNGKTKSIPVNLSVYTIEGFSGHSDRKQLIKYIRKVKPSPEKVIMVHGEESKCIDFSETVRRLFKKQTYAPNNLDAIRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSN9</accession>
    <name>N6VSN9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_03603</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the SAM hydrolase / SAM-dependent halogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96201.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591254.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSN9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01383"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.703"/>
    <dbReference type="OrthoDB" id="372224at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.90">
        <property type="entry name" value="Bacterial fluorinating enzyme like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10790">
        <property type="entry name" value="S-adenosyl-l-methionine hydroxide adenosyltransferase, N-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046470">
        <property type="entry name" value="SAM_HAT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046469">
        <property type="entry name" value="SAM_HAT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002747">
        <property type="entry name" value="SAM_OH_AdoTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023227">
        <property type="entry name" value="SAM_OH_AdoTrfase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023228">
        <property type="entry name" value="SAM_OH_AdoTrfase_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35092">
        <property type="entry name" value="CHLORINASE MJ1651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35092:SF1">
        <property type="entry name" value="CHLORINASE MJ1651"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20257">
        <property type="entry name" value="SAM_HAT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01887">
        <property type="entry name" value="SAM_HAT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006779">
        <property type="entry name" value="UCP006779"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101852">
        <property type="entry name" value="Bacterial fluorinating enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102522">
        <property type="entry name" value="Bacterial fluorinating enzyme, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="S-adenosyl-l-methionine hydroxide adenosyltransferase N-terminal" evidence="3">
        <location>
            <begin position="4"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="S-adenosyl-l-methionine hydroxide adenosyltransferase C-terminal" evidence="4">
        <location>
            <begin position="162"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00024035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96201.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="253" mass="29061" checksum="67B7F04487E8982A" modified="2013-06-26" version="1">MDIITLTTDFGYSEGYVGAMKGRILNILKKYNKEVKIIDISHDIRPFNIYHGAYVLLTSIPYFPEAVHIAVVDPTVGSERRSIVIETKNGYLVGPDNGIFSYVLDRLKVKKIYKIDENKYNPSNTFHGRDVYAVVGAEIIIRNGYDGEEIDDIVRLDDKTKRVIHIDRFGNIITNIRENEVNFSYGDDVEIEIKTRFGKKRIKCKFVRSYYEGKDFICLINSEGFLEIGRFMDSAAEKLKVDYLDEINIITSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSX3</accession>
    <name>N6VSX3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit PurL</fullName>
            <shortName evidence="8">FGAM synthase</shortName>
            <ecNumber evidence="8">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Formylglycinamide ribonucleotide amidotransferase subunit II</fullName>
            <shortName evidence="8">FGAR amidotransferase II</shortName>
            <shortName evidence="8">FGAR-AT II</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glutamine amidotransferase PurL</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purL</name>
        <name evidence="12" type="ORF">J422_03039</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer. Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the FGAMS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96301.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590752.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSX3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00155"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.596"/>
    <dbReference type="OrthoDB" id="8251at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02203">
        <property type="entry name" value="PurL_repeat1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02204">
        <property type="entry name" value="PurL_repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00420">
        <property type="entry name" value="PurL_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010074">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041609">
        <property type="entry name" value="PurL_linker"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01736">
        <property type="entry name" value="FGAM_synth_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002290">
        <property type="entry name" value="PRK01213.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43555:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18072">
        <property type="entry name" value="FGAR-AT_linker"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001587">
        <property type="entry name" value="FGAM_synthase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoribosylformylglycinamidine synthase linker" evidence="11">
        <location>
            <begin position="2"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like N-terminal" evidence="9">
        <location>
            <begin position="59"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="10">
        <location>
            <begin position="190"/>
            <end position="340"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like N-terminal" evidence="9">
        <location>
            <begin position="418"/>
            <end position="541"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="10">
        <location>
            <begin position="553"/>
            <end position="686"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="32"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="79"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="297"/>
            <end position="299"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="475"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="512"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="513"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="515"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96301.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="720" mass="79826" checksum="BAE396A006E6CF44" modified="2013-06-26" version="1">MNEKDLKYIISKLGREPNIIEKSMFENLWSEHCAYRSSKKLLKMFSKTLNKDYVIIGPGDDAAVIRLKGNICLALAMESHNHPSYIDPYNGAATGVGGIVRDVLSMGAKPIALLDPLRFGDPFGKEKDKVRWLIDGVVRGIGDYGNRIGVPTVGGECEFDSSFDYNNLVNVVCIGLVREENIIRGKAKDVGLSLILVGSTGRDGIGGASFASKDLSEESEEDRPSVQIGDAFAEKCLIDAVLEAVETGKVKAMKDLGAAGLAGAASEMCHPYVGCLLYLDKVILREPLTPYEIMISESQERMLLAVEKGSEEEIINIFKKYDLPASVIGETIENKRIIAKYNGKVVVDLPLNLLCEAPIYDREEKEDLKERLDKKPEMPNLEKTILKLLSSPNICSKKWIYEQYDYDVQLRTVVKPGREAAVLKIDELYPIGIALTTDCNSRYCKLNPYVGAMNAVAEAYRNIACVGGKPLALLDNLNFANPEKPERFWQLKECVRGLSDACYHFGIPVVGGNVSLYNETVIGNEEYPINPTPSIALIGIVKNVENSISKIDGDEILIITNDTKDEMGGSEYYKVIHNMEDGLVPRVDIEKEKVIYDAVAELKNEGIIREAIDCSRGGLAIALAKFCILNNVGLEVDLNGYNKYNLREDILLFSETSGRIILAVKEKDVDTVMERVGGYIIGKIRGEELKIKIRDKKIILSLDDMKKAYYEAFPKIMGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT64</accession>
    <name>N6VT64_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02899</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96386.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590653.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT64"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00128"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.569"/>
    <dbReference type="OrthoDB" id="11794at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd21151">
        <property type="entry name" value="PUA_Nip7-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040598">
        <property type="entry name" value="NIP7_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005155">
        <property type="entry name" value="UPF0113_PUA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17833">
        <property type="entry name" value="pre-PUA_NIP7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03657">
        <property type="entry name" value="UPF0113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="60S ribosome subunit biogenesis protein NIP7 pre-PUA" evidence="2">
        <location>
            <begin position="3"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="domain" description="UPF0113" evidence="1">
        <location>
            <begin position="94"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96386.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="159" mass="18730" checksum="3EA46080E12D6974" modified="2013-06-26" version="1">MIYRNLKKKELELIKEELERYCSKEFVEKFNFNNLIALEGKWVTVCYTNTDVLKNLKLFNEVFFIGNNFGEIKRKFRLSLEGFTLIADNIINNYCIVNEKGETLFLYGRDIFKSSILKIKGYGRIAVFNKNREFLGIGYYSGEIVKNIKDKGWYLREGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT69</accession>
    <name>N6VT69_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0280 protein J422_02924</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02924</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0280 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96391.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590662.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT69"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00133"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.574"/>
    <dbReference type="OrthoDB" id="50299at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.520.10">
        <property type="entry name" value="ApbE-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01079">
        <property type="entry name" value="UPF0280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003374">
        <property type="entry name" value="ApbE-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037456">
        <property type="entry name" value="MA1715-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007183">
        <property type="entry name" value="UPF0280"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003321">
        <property type="entry name" value="PRK04334.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006421">
        <property type="entry name" value="UCP006421"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143631">
        <property type="entry name" value="ApbE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01079"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96391.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="240" mass="26194" checksum="1FB6722762C69547" modified="2013-06-26" version="1">MISRRIIFKETNMLIKTENYLNIKLAEFIIRKCRFELENYIKTHPEFLYSLEPIDVEGKGIIKLMSIAGKIANVGPMASVAGAIAEMVVKNTFGDIIADNGGDICLRARRDINVGLYSGLSKLSGEIGFKLKKEMIKNIYGIATSSATVGHSISFGNADSVTVFAKSSAIADAAATAICNATVGKDKEEQINNALERADDIERIDGVFIVIKDLVGVKGKIPELIKTNKKVTLGELFEIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTL7</accession>
    <name>N6VTL7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ADP-ribosylation/crystallin J1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_01845</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="3">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ADP-ribosylglycohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96516.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590122.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTL7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00217"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.363"/>
    <dbReference type="OrthoDB" id="114878at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4080.10">
        <property type="entry name" value="ADP-ribosylation/Crystallin J1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050792">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005502">
        <property type="entry name" value="Ribosyl_crysJ1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036705">
        <property type="entry name" value="Ribosyl_crysJ1_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222">
        <property type="entry name" value="ADP-RIBOSYLGLYCOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR16222:SF24">
        <property type="entry name" value="ADP-RIBOSYLHYDROLASE ARH3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03747">
        <property type="entry name" value="ADP_ribosyl_GH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101478">
        <property type="entry name" value="ADP-ribosylglycohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR605502-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96516.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="292" mass="32448" checksum="D55541C262280917" modified="2013-06-26" version="1">MKDKIYGCVFGAIVGDALGAATEGMSRDEIQKVYGYVTDYVEPKNYLKGILKKGEYTDDTEQAIFIIKSINKNGSVDLKRFANYLINFGNKKFYGLTTLKAIEKLKNNDFSGVDSPTCGAAMRVYPLAILYHNNLNKLREEIIKISKITHNNKEAISGALAVGYFVANALNNKRDLDGCCSFLNNEFSNLLLKIKDFRNVQKAYNYFGTGTLAREVVPSAIATFLLTESFEEGMLKAVNAGGDTDTLASIYGAIAGAYYGYKAIPKKWIKGLKKREYIESLAEILLKHYHGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTT7</accession>
    <name>N6VTT7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Multifunctional 3-isopropylmalate dehydrogenase/D-malate dehydrogenase</fullName>
            <ecNumber evidence="8">1.1.1.85</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_01575</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.85" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589974.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTT7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00442"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.310"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019298">
        <property type="term" value="P:coenzyme B biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000019">
        <property type="entry name" value="Homoisocitrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011828">
        <property type="entry name" value="LEU3_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02088">
        <property type="entry name" value="LEU3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="7">
        <location>
            <begin position="3"/>
            <end position="325"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="332" mass="36317" checksum="84488E83F9464BDD" modified="2013-06-26" version="1">MHKICVIPGDGIGKEVVPATVKILEAVGDFEFVYAEAGDEVYKKTGKALPEETVEKCLECEAVLFGAAGETAADVIVKLRHILDTYANVRPVKAYKGVKCLRDDIDYVIVRENTEGLYKGIEAKIDEGITIATRVITEKACERIFRFAFELARDRKKQGKEGKVTCAHKANVLKITDGLFKDVFYKIAKEYPDIKAEDYYIDAMNMYIITKPQTFDVVVTSNLFGDILSDGAAGTVGGLGLAPSANIGDEHGLFEPVHGSAPDIAGKGIANPTATILSAVLMLRYLNEHEMANKIEKALEEVLAEGLTTPDLGGNLKTFEMAEEIIKRLEHD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTU2</accession>
    <name>N6VTU2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Probable Brix domain-containing ribosomal biogenesis protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_01600</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Probably involved in the biogenesis of the ribosome.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96576.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589979.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTU2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00447"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.315"/>
    <dbReference type="OrthoDB" id="117530at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10480">
        <property type="entry name" value="Probable brix-domain ribosomal biogenesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00699">
        <property type="entry name" value="BriX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007109">
        <property type="entry name" value="Brix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023548">
        <property type="entry name" value="Brix_dom_Rbsml_bgen_prot"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002094">
        <property type="entry name" value="PRK00933.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00879">
        <property type="entry name" value="Brix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50833">
        <property type="entry name" value="BRIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0690">Ribosome biogenesis</keyword>
    <feature type="domain" description="Brix" evidence="3">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96576.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="170" mass="20358" checksum="6706B2F01262C06D" modified="2013-06-26" version="1">MILTSSRKPSQRTRSFLRDLERTLNIPYIQRGKLSLKELFEMDKHILLIGEFKGNPGTLMVYDVEREKKLSSFISVKLQREICGEKIYNKDGIKLKVDKRLEDDENFKEFYDIYDKFLFQHLNIDDQSEIVLRLEKDPKYLFAMQFYKNRVKIGPLIRIKSIKLFDKLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU16</accession>
    <name>N6VU16_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">5-methylcytosine restriction system component-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01021</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000006">
        <property type="protein sequence ID" value="ENN96676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589872.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU16"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01014"/>
    <dbReference type="REBASE" id="68594">
        <property type="entry name" value="MviT80McrBCP"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.199"/>
    <dbReference type="OrthoDB" id="62357at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019292">
        <property type="entry name" value="McrC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38733">
        <property type="entry name" value="PROTEIN MCRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38733:SF1">
        <property type="entry name" value="TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMCRBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10117">
        <property type="entry name" value="McrBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="405" mass="48717" checksum="DEE8CB0E9C058A93" modified="2013-06-26" version="1">MLITFYEYQEKDVEELKNELNLNEKEIFNLFKKINGGSEDKIFTLYYNKIKANNYVGFASIKDVSIQILPKIFKNSEEKEYLFLNMLNFAFDLKLEEKEVKAINSLSKAFYEIFIYLFAKSLLEEIKRGIYKDYIKIRGEEKFLKGKLLIEKEIRKLPHQRDRFFVEYFLFDENNLLNQIFYYTVITSLRKTKIRINKKYLSELMLIFDGIELKKINVEDFKKVKFTRLNNRFKKSFNLAKILLTSLGELRGEDAVGFFIDMNELFERFICNILKRKFSIGYQEEFNLLKETKYLDKIKQKPDFIVYKNGEVKFVLDAKYKEIEKNPSPDILRQIYTYAKKYKVPSALIFPKFKNYNNFKTIVDHCKFFDGTEIYLLVYNLETLKNNEIDKEFLNAINKLLNKFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VUF4</accession>
    <name>N6VUF4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PAS/PAC sensor protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00225</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96826.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589732.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VUF4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01124"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.45"/>
    <dbReference type="OrthoDB" id="6433at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07709">
        <property type="entry name" value="flavodiiron_proteins_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045761">
        <property type="entry name" value="ODP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000014">
        <property type="entry name" value="PAS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016440">
        <property type="entry name" value="Rubredoxin-O_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43717">
        <property type="entry name" value="ANAEROBIC NITRIC OXIDE REDUCTASE FLAVORUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43717:SF1">
        <property type="entry name" value="ANAEROBIC NITRIC OXIDE REDUCTASE FLAVORUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19583">
        <property type="entry name" value="ODP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005243">
        <property type="entry name" value="ROO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00091">
        <property type="entry name" value="PAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="263"/>
            <end position="404"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96826.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="412" mass="46739" checksum="8F529CA35D80301E" modified="2013-06-26" version="1">MKADAVKIADGVYWVGVLDWDIRVYHGYTLKGTTYNAYLVFGDEKVAIIDNTYPGTSAQMWARIKDAFEKEGREFKIDVIVQNHVEKDHSGALPEIHKKFPEAPIYCTEVAVKGLKKHYPSLENAPFKVVKTGDTVDLGGKTLTFLEAPLLHWPDSMFTFYNEGGILFSNDAFGQHLCYPAHKRFDKDIPEYVLMDANQKFYANLITPLSKLVLKKFEEVKNLGLLEKIKMIAPSHGQIWTDPMKVIKAYQDFATGKAAKDKVTIVYDTMHYSTQKMAHAFAEGLMSEGIDVVMYFLHYDERSEIVKDILDSKALLLGAPTIYDEPYPSVGDLIYYLRGLKFNRAGFKRLALVFGSMGGEGGAVNRLEEELKSCGFEVIDKYELYYVPTKDELENCYNMGKRLAAKIKDMKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>N6VWT9</accession>
    <name>N6VWT9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Glycerol-1-phosphate dehydrogenase [NAD(P)+]</fullName>
            <shortName evidence="11">G1P dehydrogenase</shortName>
            <shortName evidence="11">G1PDH</shortName>
            <ecNumber evidence="11">1.1.1.261</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Enantiomeric glycerophosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">sn-glycerol-1-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">egsA</name>
        <name evidence="15" type="ORF">J422_07202</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the NAD(P)H-dependent reduction of dihydroxyacetonephosphate (DHAP or glycerone phosphate) to glycerol 1-phosphate (G1P). The G1P thus generated is used as the glycerophosphate backbone of phospholipids in the cellular membranes of Archaea.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>sn-glycerol 1-phosphate + NAD(+) = dihydroxyacetone phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>sn-glycerol 1-phosphate + NADP(+) = dihydroxyacetone phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21416"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.261"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 12">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the glycerol-1-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.261" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000079">
        <property type="protein sequence ID" value="ENN95557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004595203.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000079.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VWT9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00790"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1391"/>
    <dbReference type="OrthoDB" id="8656at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106357">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106358">
        <property type="term" value="F:glycerol-1-phosphate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006650">
        <property type="term" value="P:glycerophospholipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd08173">
        <property type="entry name" value="Gro1PDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1090.10">
        <property type="entry name" value="Dehydroquinate synthase-like - alpha domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00497_A">
        <property type="entry name" value="G1P_dehydrogenase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023002">
        <property type="entry name" value="G1P_dehydrogenase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032837">
        <property type="entry name" value="G1PDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016205">
        <property type="entry name" value="Glycerol_DH"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616">
        <property type="entry name" value="GLYCEROL DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43616:SF5">
        <property type="entry name" value="GLYCEROL DEHYDROGENASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13685">
        <property type="entry name" value="Fe-ADH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000112">
        <property type="entry name" value="Glycerol_dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56796">
        <property type="entry name" value="Dehydroquinate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="3 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 11 14" id="KW-0520">NAD</keyword>
    <keyword evidence="5 11" id="KW-0521">NADP</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9 11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 12" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="11 14">
        <location>
            <begin position="71"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <begin position="93"/>
            <end position="96"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="98"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="141"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="240"/>
        </location>
        <ligand>
            <name>glycerol</name>
            <dbReference type="ChEBI" id="CHEBI:17754"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000112-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN95557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="327" mass="36660" checksum="1BCB2625FD27F795" modified="2013-06-26" version="1">MRIITPRYTIIEDHAINHLNDILKKLSINNPLVITGKKTKKFAPKEFDFIYYSELPERISGYDAIIGVGGGRAIDYGKYLAYKNDLPFISIPTTASNDGIASPIVSIKQPSFMTVSPIAIIVDTKIIEKSPKRLLASGFGDIVSNITAVLDWQLAYKEKGEKYSESSAIFSKTVAEEILNYVLSSDLKEFHKKLAKALVGSGIAIAIANSSRPASGSEHLFSHALDYLKEKYKLDVDSLHGEQCGFGAIIMSYLHEKENKNLTNLHIRIREALRKVKAPTTIEELGFDKEVIIEALSIAHKMRDRWTILREGISKEKARKIIEELFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6VWY6</accession>
    <name>N6VWY6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13">GMP synthase [glutamine-hydrolyzing] subunit B</fullName>
            <ecNumber evidence="4 13">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 13">GMP synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">guaAB</name>
        <name evidence="16" type="ORF">J422_06922</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 13">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 13">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 13">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="4 13"/>
    <dbReference type="EMBL" id="APMM01000064">
        <property type="protein sequence ID" value="ENN95612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594597.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VWY6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00933"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1342"/>
    <dbReference type="OrthoDB" id="33844at2157"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01997">
        <property type="entry name" value="GMP_synthase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.10:FF:000002">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000208">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing] subunit B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00345">
        <property type="entry name" value="GMP_synthase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001674">
        <property type="entry name" value="GMP_synth_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026598">
        <property type="entry name" value="GMP_synthase_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025777">
        <property type="entry name" value="GMPS_ATP_PPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00884">
        <property type="entry name" value="guaA_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000848">
        <property type="entry name" value="PRK00074.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00958">
        <property type="entry name" value="GMP_synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51553">
        <property type="entry name" value="GMPS_ATP_PPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 13 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 13 14" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="6 13 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 13 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 13 14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GMPS ATP-PPase" evidence="15">
        <location>
            <begin position="2"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="29"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049404"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN95612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="308" mass="34627" checksum="83EAD0446EAFB427" modified="2013-06-26" version="1">MFNPKEFIEEAVKEIKEKVKDRKAIIALSGGVDSSVAAILAYKAIGDNLTAVFVDTGLMRKGEREEVEKVFKSIGLNLIIVDAKERFLKALKGVRDPEEKRRIIGRLFIEVFEEVARDIGAEVLIQGTIAPDWIETEGKIKSHHNVSLPSGMVLEVVEPIRELYKDEVRILAKELGLPDSIVYRQPFPGPGLAVRVLGEVTEEKLEICREANAIVEEEVKKANLDKELWQYFAAVLDCKATGVKGDEREYSWVVAVRMVKSIDAMTAHVPEIDFNILKRIAKRITSELNVSRVLFDITDKPPATIEFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX01</accession>
    <name>N6VX01_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Phosphoesterase RecJ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_06802</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000062">
        <property type="protein sequence ID" value="ENN95632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594211.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX01"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01091"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1320"/>
    <dbReference type="OrthoDB" id="36101at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053584">
        <property type="entry name" value="RecJ_exonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051673">
        <property type="entry name" value="SSDNA_exonuclease_RecJ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040701">
        <property type="entry name" value="RecJ_Meth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255:SF3">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDH" evidence="2">
        <location>
            <begin position="19"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="3">
        <location>
            <begin position="356"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="320"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="458" mass="52502" checksum="B5421F8A37B6CA4D" modified="2013-06-26" version="1">MFKELNKAKEIFLKNKNNKVLIATHIDTDGLTSMVILKKIVERLEVDADFIFLKQINEETIDDIDFDYDLIILADFGSGQLSIIKEKIEEVRYNKKIIILDHHQIEKERVENIILVNPLTENKETCGAGVCYLFAKAINSNWIDLAKYAVIGAVGDVQNIEGKLKSLNREILCDAIFAGDVVIKNDLQLYGRQTRPLFVSLRYWADVRTDLLDNDLNIIKYINYINKKYGIDIDPTKSLAKLPYSYKKIIGNELLIKSLQYVPPHWAKYVPKVIFGEVYELSYEDEGSYLKDLEELSTCINSCSRYGDYETAINILLGNKKYYNKMIKNLERHRKNLREALNHIKTEVEIKKEKNFQYFETDKIKPNIIGIVAGMSYTIDEIDWQKPIFAIAEDENSYKVSARCPKLLCFAENIDLGKAIKIASEKANGSGGGHKFASGAYIKNKEEFIKCLSEALKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX28</accession>
    <name>N6VX28_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Nitrate/sulfonate/bicarbonate ABC transporter periplasmic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06643</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000056">
        <property type="protein sequence ID" value="ENN95667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593811.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX28"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00368"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1293"/>
    <dbReference type="OrthoDB" id="10037at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30024">
        <property type="entry name" value="ALIPHATIC SULFONATES-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13379">
        <property type="entry name" value="NMT1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="381" mass="42767" checksum="23823095C1C8C162" modified="2013-06-26" version="1">MRKTMVILISLLFVSAAIALAGCQEKKVVEENNGVVKISVGYLPTDHHAALFVACYHPEIFKKYGIYLKEVEPKKKYELYKNDKKIADIELYLIKTGGADIMNLLAQGKLDIGLNGVPPAVFYIDKGCNGKIIMCLQGEGSAVVVRKDIPANNWQEFVNWIKEQYKEGKQVKIGHPLPTSIQYVMIKDALKASGISYTENPNEKAMVLLVNCKGQGSMPQMLSQKQLDAVIAWEPMPEILKSKGVGKAIVYSQDLPKSNGGTWKNHPCCCVVASEKSLKEKRDAIITFAKLLRYATEEINKNRDLAAEASTKWLGVDENVEKESVKYIKYDWRIETFLPGILEFVKAMNNQGLMKNMLKDVSDEKAKEVIFDFNAYNEIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX32</accession>
    <name>N6VX32_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Histone deacetylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000055">
        <property type="protein sequence ID" value="ENN95672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593718.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX32"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00605"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1278"/>
    <dbReference type="OrthoDB" id="147549at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004407">
        <property type="term" value="F:histone deacetylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0040029">
        <property type="term" value="P:epigenetic regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.20">
        <property type="entry name" value="Histone deacetylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000286">
        <property type="entry name" value="HDACs"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023801">
        <property type="entry name" value="His_deacetylse_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037138">
        <property type="entry name" value="His_deacetylse_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625:SF10">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10625">
        <property type="entry name" value="HISTONE DEACETYLASE HDAC1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00850">
        <property type="entry name" value="Hist_deacetyl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01270">
        <property type="entry name" value="HDASUPER"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Histone deacetylase" evidence="1">
        <location>
            <begin position="17"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="315" mass="36664" checksum="9004E0CCD415779A" modified="2013-06-26" version="1">MILYSDNVLNHRPHTYHIENPKRVEIILETLKEHGYDEIFYVDEKVSLREVLTIHDEEYINKLLSLKEFTFLDPDTYFSEGTLEAALTSLKMSEIAFKLSLKEKDLSFALTRPPGHHSGRKGWAGPTLGFCIFNNIAYAVKLAKDYFEKVIIIDFDAHHGNGTEDIFKEDDDIIIIDFHQKGIYPFTGNESYRTKINLPFISAKDDDYIYTYYEIVEPILEYYKPKIIFVSAGFDGYQGDLTDLKLKDFFQFIGYKLSDYPVCAVLEGGYFCGIKQGPIKFLNGYYHDFKERDINPSTYTKEMVNRVKSDILWLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXA9</accession>
    <name>N6VXA9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Agmatinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_06145</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="4">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arginase family. Agmatinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593257.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXA9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00548"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1197"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008783">
        <property type="term" value="F:agmatinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033389">
        <property type="term" value="P:putrescine biosynthetic process from arginine, via agmatine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11593">
        <property type="entry name" value="Agmatinase-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.800.10">
        <property type="entry name" value="Ureohydrolase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005925">
        <property type="entry name" value="Agmatinase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006035">
        <property type="entry name" value="Ureohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023696">
        <property type="entry name" value="Ureohydrolase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020855">
        <property type="entry name" value="Ureohydrolase_Mn_BS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01230">
        <property type="entry name" value="agmatinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358">
        <property type="entry name" value="ARGINASE/AGMATINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11358:SF26">
        <property type="entry name" value="GUANIDINO ACID HYDROLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00491">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036979">
        <property type="entry name" value="Arginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52768">
        <property type="entry name" value="Arginase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01053">
        <property type="entry name" value="ARGINASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51409">
        <property type="entry name" value="ARGINASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="4">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="130"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRSR" id="PIRSR036979-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="281" mass="31934" checksum="61AC1DFAD4D9FE70" modified="2013-06-26" version="1">MFIDLSKYMLADADYKSADGVIISIPYDETTSFKPGARDGGKAIREASWGLESYSPILDRDLTEVKYCDLKDLDLYGNQKDIFNLITNTIKNILNDGKKPIIFGGEHSITFPIIKAVKEIYNDFIVIQFDAHCDLRDEYLGNPLSHACVMRRIYEINKNIFQFGVRSGDKEEWEFIKKCNIYSKQRLLNEEDIDIIKDINKPIYVTIDIDVLDPAFAPGTGTPEPCGFTTKELFNSLYLLRDVKDMIVGFDIVEVAPIYDLANITAITAAKIARELMLMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXF3</accession>
    <name>N6VXF3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11">Translation initiation factor 5A</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">Hypusine-containing protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">eIF-5A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">eif5a</name>
        <name evidence="13" type="ORF">J422_05693</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Functions by promoting the formation of the first peptide bond.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the eIF-5A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592791.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXF3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01264"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1110"/>
    <dbReference type="OrthoDB" id="23689at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045901">
        <property type="term" value="P:positive regulation of translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045905">
        <property type="term" value="P:positive regulation of translational termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04467">
        <property type="entry name" value="S1_aIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000038">
        <property type="entry name" value="Translation initiation factor 5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00085">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001884">
        <property type="entry name" value="IF5A-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048670">
        <property type="entry name" value="IF5A-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020189">
        <property type="entry name" value="IF5A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022847">
        <property type="entry name" value="Transl_elong_IF5A_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00037">
        <property type="entry name" value="eIF_5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003076">
        <property type="entry name" value="PRK03999.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11673">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR 5A FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01287">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21485">
        <property type="entry name" value="IF5A-like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003025">
        <property type="entry name" value="eIF5A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01376">
        <property type="entry name" value="eIF-5a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 11" id="KW-0385">Hypusine</keyword>
    <keyword evidence="6 11 13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor 5A C-terminal" evidence="12">
        <location>
            <begin position="70"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="modified residue" description="Hypusine" evidence="11">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN95807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="129" mass="13970" checksum="22455C3ABEF29C35" modified="2013-06-26" version="1">MPGTKQVNVGSLKVGQYVMIDGVPCEIVDISISKSGKHGGAKARVVGIGIFDKVKKEFVAPTSAKVEVPIIDRRKGQVLAVVGDTVQIMDLQTYETLELPIPEGLELEPGKEVEYIEAVGQYKITRVFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXX9</accession>
    <name>N6VXX9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">TrkA-N domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_05100</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95992.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592154.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXX9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00469"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1000"/>
    <dbReference type="OrthoDB" id="43518at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="entry name" value="Regulator of K+ conductance, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050721">
        <property type="entry name" value="Trk_Ktr_HKT_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833:SF13">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="65"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="3">
        <location>
            <begin position="108"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="4">
        <location>
            <begin position="247"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95992.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="332" mass="36936" checksum="199A0D3A19675DB8" modified="2013-06-26" version="1">MQDSIKKIFFGILSLLLLIFIQTLVYSSIEGWDLFTSFYVSVITISTVGYGDFVPKTFLGRVVTIIYIFVGVGLVAYTLSNVASFLIEGHFKKIFKMRKMIEKIKKLKDHYIVCGYGRLGRVVVDELKKSNIPYVVIDINEDVLKEAVEMDNDLICIVGDATLEETLEKANIRKAKGLISVVGSDAENVFITLTAKSLNPNIFVVAKADKSSTLDKLIKAGADRAICPYIVGGMEIAKIAINPEILEFIHSLVANEEDMEVRRLTIKNEALDGKTLRESKIREKTGATILAIKKGNKMITSPPPEVKLNVGDVVYAFGKREQLEKLSKYLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6VY00</accession>
    <name>N6VY00_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Restriction endonuclease type IV Mrr domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04705</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96012.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591981.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VY00"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00960"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.920"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96012.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="213" mass="25422" checksum="627024FD2E71E843" modified="2013-06-26" version="1">MIEDVIKGEKKIKVKIGNYMLEGIKLEEENKLYEFFNLALNKLRYRKAIFNNFLLSEIKNINRLQKIHEIDEYFLKLLDELNKEINLMSLSKGIIFELFICYSFFILFSDIEVMRNLNVYYNNRHFTEIDMLLNGKNRIVGECKNRAIFANDILKLFGLITTLNADFGLLISSKKFNIIKKEEVFYEYNIYILDNLFEKDKNKIYKEVKSLVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYH7</accession>
    <name>N6VYH7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rps17p</name>
        <name evidence="7" type="synonym">rps17</name>
        <name evidence="9" type="ORF">J422_03953</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">One of the primary rRNA binding proteins, it binds specifically to the 5'-end of 16S ribosomal RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96177.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591400.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYH7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00420"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.775"/>
    <dbReference type="OrthoDB" id="10698at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00364">
        <property type="entry name" value="Ribosomal_uS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.1000:FF:000005">
        <property type="entry name" value="30S ribosomal protein S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.1000">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01345_A">
        <property type="entry name" value="Ribosomal_uS17_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000266">
        <property type="entry name" value="Ribosomal_uS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028333">
        <property type="entry name" value="Ribosomal_uS17_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019978">
        <property type="entry name" value="Ribosomal_uS17_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019979">
        <property type="entry name" value="Ribosomal_uS17_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004123">
        <property type="entry name" value="PRK05610.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006345">
        <property type="entry name" value="PRK08572.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03630">
        <property type="entry name" value="uS17_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10744:SF9">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00366">
        <property type="entry name" value="Ribosomal_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00973">
        <property type="entry name" value="RIBOSOMALS17"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00056">
        <property type="entry name" value="RIBOSOMAL_S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96177.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="116" mass="13225" checksum="C8DE55301647B0E8" modified="2013-06-26" version="1">MARNIGIKVKAPEVECNDENCPFHGTLPVRGQSFVGVVVSDKMQKTVVVKREYLHYLKKYERYEKRSSKLHAHNPPCINAKVGDIVRIMECRPISKTKSFVVVEKLGNIKDIKGEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYM3</accession>
    <name>N6VYM3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03483</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000022">
        <property type="protein sequence ID" value="ENN96227.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591146.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYM3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01337"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.679"/>
    <dbReference type="OrthoDB" id="8915at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF1">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="38"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96227.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="242" mass="28291" checksum="88C2F46A3BEB104B" modified="2013-06-26" version="1">MWECYAKKVPYFLDLSIPDMLKNLLKELNKRRTYSLIDLGCGDGRFLYALYKQGLLKNATRVVGVDISEERIRRLKKLCPFAEGIVADVTDLSQIPDNSFDVAISNQVIEHVPDDSKMLKEIRKILKPNGLLYISTVIKKWYGFWIYWKNGFKLDPTHVREYMSEEEFLNLLENNGFMVKEFKTSPIRYPIMDLILRGLIKLNIIKPDPAFYQKYKLISKLKKLKLKVIGYKSIEVVAEVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYM8</accession>
    <name>N6VYM8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">mannose-1-phosphate guanylyltransferase</fullName>
            <ecNumber evidence="2">2.7.7.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_03508</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>alpha-D-mannose 1-phosphate + GTP + H(+) = GDP-alpha-D-mannose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:15229"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57527"/>
            <dbReference type="ChEBI" id="CHEBI:58409"/>
            <dbReference type="EC" id="2.7.7.13"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the mannose-6-phosphate isomerase type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.13" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000022">
        <property type="protein sequence ID" value="ENN96232.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591154.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYM8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01332"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.684"/>
    <dbReference type="OrthoDB" id="5825at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004475">
        <property type="term" value="F:mannose-1-phosphate guanylyltransferase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009298">
        <property type="term" value="P:GDP-mannose biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02213">
        <property type="entry name" value="cupin_PMI_typeII_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02509">
        <property type="entry name" value="GDP-M1P_Guanylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000046">
        <property type="entry name" value="Mannose-1-phosphate guanylyltransferase (GDP)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.10:FF:000032">
        <property type="entry name" value="Mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049577">
        <property type="entry name" value="GMPP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006375">
        <property type="entry name" value="Man1P_GuaTrfase/Man6P_Isoase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001538">
        <property type="entry name" value="Man6P_isomerase-2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054566">
        <property type="entry name" value="ManC/GMP-like_b-helix"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051161">
        <property type="entry name" value="Mannose-6P_isomerase_type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01479">
        <property type="entry name" value="GMP_PMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46390">
        <property type="entry name" value="MANNOSE-1-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46390:SF1">
        <property type="entry name" value="MANNOSE-1-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22640">
        <property type="entry name" value="ManC_GMP_beta-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01050">
        <property type="entry name" value="MannoseP_isomer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 12" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="domain" description="MannoseP isomerase/GMP-like beta-helix" evidence="11">
        <location>
            <begin position="273"/>
            <end position="326"/>
        </location>
    </feature>
    <feature type="domain" description="Mannose-6-phosphate isomerase type II C-terminal" evidence="10">
        <location>
            <begin position="331"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96232.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="449" mass="51991" checksum="02D29F154F98799D" modified="2013-06-26" version="1">MISIILAGGKGTRLWPLSREYFSKQFLDFGFGKSLFQMTVERCLEFSGLNDIFVVTNEKYKFIVLNQLEELGYNFKEENILLEPESKNTLPAIYYAIKDLNNDEIVGVFPSDHIIKGDLKEYIEKAKKIANNYIVIFGVKPNKPHTGYGYIKPGEKLEVGYKVDEFKEKPNIEKAKEYLKKGYLWNSGMFLFKKDVFVEEVKNHCKEVYEAFLLDDIKEAYKTVPDISIDYGVIEKSKKVAVIPLEIKWNDLGSFDALYDEFSKDKNGNVILGDNIILDSKNNLSINKKLIALIDINDLIVVDTRDALLICKKGSSQKVKEVVKKLKEINDERAIIHKKVFRPWGYYKVLEEGERFKIKRITVLPGKRLSYQMHYHRSEHWIVVKGTAKVVIEGKEYLIRAGESIFVKSGLKHRLENPGKIPLEVIEIQVGEYLEEDDIVRFDDDYNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYY7</accession>
    <name>N6VYY7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">2,5-diamino-6-(ribosylamino)-4(3H)-pyrimidinone 5'-phosphate reductase</fullName>
            <ecNumber evidence="9">1.1.1.302</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_02639</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NAD(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27274"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>2,5-diamino-6-(1-D-ribitylamino)pyrimidin-4(3H)-one 5'-phosphate + NADP(+) = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27278"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58890"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="1.1.1.302"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HTP reductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.302" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96342.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590563.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYY7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00080"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.517"/>
    <dbReference type="OrthoDB" id="10178at2157"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008703">
        <property type="term" value="F:5-amino-6-(5-phosphoribosylamino)uracil reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.430.10">
        <property type="entry name" value="Dihydrofolate Reductase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024072">
        <property type="entry name" value="DHFR-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006401">
        <property type="entry name" value="Rib_reduct_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011549">
        <property type="entry name" value="RibD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002734">
        <property type="entry name" value="RibDG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050765">
        <property type="entry name" value="Riboflavin_Biosynth_HTPR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01508">
        <property type="entry name" value="rib_reduct_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00227">
        <property type="entry name" value="ribD_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38011:SF7">
        <property type="entry name" value="2,5-DIAMINO-6-RIBOSYLAMINO-4(3H)-PYRIMIDINONE 5'-PHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38011">
        <property type="entry name" value="DIHYDROFOLATE REDUCTASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_8G06820)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01872">
        <property type="entry name" value="RibD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53597">
        <property type="entry name" value="Dihydrofolate reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0521">NADP</keyword>
    <keyword evidence="6 11" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="domain" description="Bacterial bifunctional deaminase-reductase C-terminal" evidence="10">
        <location>
            <begin position="3"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR01508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96342.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="215" mass="23929" checksum="3AD4F42A91A420FF" modified="2013-06-26" version="1">MKPYVISNVGMSLDGKLATVTGDSRISCEEDLIRVHKIRAKVDAIMVGIGTVLKDDPRLTVHKIKADKNPIRIVVDSKLRIPLNARVLNNEAKTIIATTEKADKEKMKLLKERGVEVIVCGRDKVDLKELMKILYNKGIRSILLEGGGTLNWGMFKEGLVDEVSVYIAPMIFGGKDAVSYVDGEGFKTVEEAVKLKLKKFYRLGEGIVLEYVVLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ36</accession>
    <name>N6VZ36_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">dUTP pyrophosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02929</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96392.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590664.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ36"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00134"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.575"/>
    <dbReference type="OrthoDB" id="45265at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008829">
        <property type="term" value="F:dCTP deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006229">
        <property type="term" value="P:dUTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07557">
        <property type="entry name" value="trimeric_dUTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.70.40.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011962">
        <property type="entry name" value="dCTP_deaminase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036157">
        <property type="entry name" value="dUTPase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033704">
        <property type="entry name" value="dUTPase_trimeric"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002598">
        <property type="entry name" value="PRK02253.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680">
        <property type="entry name" value="DCTP DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42680:SF1">
        <property type="entry name" value="DEOXYURIDINE 5'-TRIPHOSPHATE NUCLEOTIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22769">
        <property type="entry name" value="DCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51283">
        <property type="entry name" value="dUTPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96392.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="156" mass="18361" checksum="B79001EA57BC5A22" modified="2013-06-26" version="1">MIIGPEESKKFLKDIKDEQIQQCGIDLRVWKIYEMEGVGEIDFSNEKRKLPNYREIFNSENDEFILLDRGVYLLKVYDYIKIPENVAAITLPRSSLLRMGATIYSAVHDPGYEGRPEYLLHVLNPIKIYKFARIGQIVFIRGEYIKNLYNGIYKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZT4</accession>
    <name>N6VZT4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01455</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000010">
        <property type="protein sequence ID" value="ENN96587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589952.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZT4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01025"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.285"/>
    <dbReference type="OrthoDB" id="65659at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="162" mass="18298" checksum="1FA0A1CA6882743A" modified="2013-06-26" version="1">MLKKMIILVGILLLISAIYGEALNLGSLNVTKKQKMKNISFTINKVKNQKYTLNFIHYGNINENMKVYLYLNDNLIYTIGDSNDGSGHYKKRKSLDITKYLNDGSNILKLEGENLNTANYSVYYKLENITISTPTTKTPIPLGAVILTLIAIPLIILRKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZU9</accession>
    <name>N6VZU9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-export membrane protein SecF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">secF</name>
        <name evidence="11" type="ORF">J422_01363</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Involved in protein export.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the protein translocation apparatus. Forms a complex with SecD.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the SecD/SecF family. SecF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZU9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00016"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.267"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1640.10">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01464_A">
        <property type="entry name" value="SecF_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022813">
        <property type="entry name" value="SecD/SecF_arch_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048634">
        <property type="entry name" value="SecD_SecF_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024921">
        <property type="entry name" value="SecF_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006353">
        <property type="entry name" value="PRK08578.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006357">
        <property type="entry name" value="PRK08578.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081:SF8">
        <property type="entry name" value="PROTEIN TRANSLOCASE SUBUNIT SECF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30081">
        <property type="entry name" value="PROTEIN-EXPORT MEMBRANE PROTEIN SEC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02355">
        <property type="entry name" value="SecD_SecF_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82866">
        <property type="entry name" value="Multidrug efflux transporter AcrB transmembrane domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 9" id="KW-0653">Protein transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 9" id="KW-0811">Translocation</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="44"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="161"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="184"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="280"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="domain" description="Protein export membrane protein SecD/SecF C-terminal" evidence="10">
        <location>
            <begin position="127"/>
            <end position="310"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="312" mass="34699" checksum="53CB2BAFD4F7DD40" modified="2013-06-26" version="1">MLGRNRRRISNSFLITITSMYIYDFMILYKKIINQRGIMLIKDYKVAISVPIFLIFISLMLITLKGIPKSIDITGGTEITIKLNKDIDLEKLKEMLKGEGELEILKSASGNYLVIRCKSEYLNDVKAKLREFFGDLNKLDYSEKTVGSVLSKKFFEEGGKAMLFAFIFMALAVYIAFRKPLASGVIILSALADLINTLGAMSLLNIQLSTATIAALLMVLGYSVDSNILLTTRVLKRTKDFDTAAKEAMKTGLTMITTTIVAMLILYIVVKLFIPVADVLATISSIIIIALLFDIINTWMLNAGILKAFSKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZY2</accession>
    <name>N6VZY2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_01096</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589887.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZY2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00823"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.214"/>
    <dbReference type="OrthoDB" id="105445at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005909">
        <property type="entry name" value="RaSEA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01210">
        <property type="entry name" value="archaeosine biosynthesis radical SAM protein RaSEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF0">
        <property type="entry name" value="ELONGATOR COMPLEX PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004954">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="16"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="330" mass="38472" checksum="1F0F26AF992CB559" modified="2013-06-26" version="1">MKPIAVWLKDEIYHDFSIGKCLVIILKTIGCSYNRCIFCSYKEDASKDIKEEEIIGQFDHALKKYNLEELDSFSVKIFTSGSFLDDREVSKRVRRYIYERLSQYKNLKEFSIESRAEYITEDKLDEMRKYLSCHIDIGLGIETFNEELRELLNKGLKCEDIVKAIRIAKKYDVGIKAYLLIKPPFLTERDAIKDAISSAKKAFELGCSRISFCPITIHKNTVLEYLWKKGQYRPPFLWSVLEIIRSVKEHYPKKIIVCDTTAFGLKRGAHNIIGCGCNKIIKENIEKFNLYQTLDLLDVECECKKVYESYIIAENKNIVPFIQECDLLEP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6W008</accession>
    <name>N6W008_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Small ribosomal subunit protein eS24</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 4" type="primary">rps24e</name>
        <name evidence="4" type="ORF">J422_00886</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eS24 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589847.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W008"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00597"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.174"/>
    <dbReference type="OrthoDB" id="27533at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00545">
        <property type="entry name" value="Ribosomal_eS24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001976">
        <property type="entry name" value="Ribosomal_eS24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01282">
        <property type="entry name" value="Ribosomal_S24e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 4" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="102" mass="11924" checksum="355700E5B81065C4" modified="2013-06-26" version="1">MEIKIVSDRYNPLLKRREIKFILDHEGATPTFKDVRSKLCAMFNVDKNLLILEKIVEETGMQRCRGYVKIYDDENILKLVERKHILLKNEIGGENDQGEESS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6W090</accession>
    <name>N6W090_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein-L-isoaspartate O-methyltransferase</fullName>
            <ecNumber evidence="9">2.1.1.77</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">L-isoaspartyl protein carboxyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein L-isoaspartyl methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Protein-beta-aspartate methyltransferase</fullName>
            <shortName evidence="9">PIMT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pcm</name>
        <name evidence="10" type="ORF">J422_00431</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>[protein]-L-isoaspartate + S-adenosyl-L-methionine = [protein]-L-isoaspartate alpha-methyl ester + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:12705"/>
            <dbReference type="Rhea" id="RHEA-COMP:12143"/>
            <dbReference type="Rhea" id="RHEA-COMP:12144"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90596"/>
            <dbReference type="ChEBI" id="CHEBI:90598"/>
            <dbReference type="EC" id="2.1.1.77"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the methyltransferase superfamily. L-isoaspartyl/D-aspartyl protein methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.77" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589768.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W090"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00694"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.83"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004719">
        <property type="term" value="F:protein-L-isoaspartate (D-aspartate) O-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030091">
        <property type="term" value="P:protein repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000010">
        <property type="entry name" value="Protein-L-isoaspartate O-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00090">
        <property type="entry name" value="PIMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000682">
        <property type="entry name" value="PCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00080">
        <property type="entry name" value="pimt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001453">
        <property type="entry name" value="PRK00312.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010549">
        <property type="entry name" value="PRK13942.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11579:SF0">
        <property type="entry name" value="PROTEIN-L-ISOASPARTATE(D-ASPARTATE) O-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01135">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01279">
        <property type="entry name" value="PCMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9 10" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="9">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="211" mass="23507" checksum="F47699EEE60BD080" modified="2013-06-26" version="1">MDKKEVIKRLIEEGYIKSEKVIKALLKVPREEFVPEHLRDYAYVDEPLPIGYNQTISAIHMVGLMCELLDLDRGMKVLEIGTGSGYHAAVVAEIVGKEGKVISIERIPELAKRAEEILKRLGYDNVKVIVGDGSLGYEKEAPYDRIYVTAAGPKIPEALIRQLKDGGKLLMPVGKYFQKLILVEKRGDKIIKKDCGAVAFVPLIGKEGFNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTE9</accession>
    <name>N6UTE9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CheW protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_07112</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000074">
        <property type="protein sequence ID" value="ENN95574.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594970.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTE9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01214"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1376"/>
    <dbReference type="OrthoDB" id="115049at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.180">
        <property type="entry name" value="CheA-289, Domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039315">
        <property type="entry name" value="CheW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617:SF23">
        <property type="entry name" value="CHEMOTAXIS PROTEIN CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22617">
        <property type="entry name" value="CHEMOTAXIS SENSOR HISTIDINE KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="CheW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheW-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95574.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="152" mass="16878" checksum="6A6BF0AC4966B322" modified="2013-06-26" version="1">MGNENDVIKIVVFRLGKNEYGLKVDEVREVLKLKKSDITPLPNAPSYVVGVTNIRGEITPIINLKEVLGIYEEVENGKKDEKLVMVIEIDQQTFGILVDGVNDVMQITSDMITPVSAIKKSTLGGDYVEGIVKIENRLIILLNISNLLNLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTH5</accession>
    <name>N6UTH5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Hydroxylamine reductase</fullName>
            <ecNumber evidence="8">1.7.99.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Hybrid-cluster protein</fullName>
            <shortName evidence="8">HCP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Prismane protein</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">hcp</name>
        <name evidence="9" type="ORF">J422_06827</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reduction of hydroxylamine to form NH(3) and H(2)O.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>A + NH4(+) + H2O = hydroxylamine + AH2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22052"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15429"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="1.7.99.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="8">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </cofactor>
        <text evidence="8">Binds 1 hybrid [4Fe-2O-2S] cluster.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the HCP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.7.99.1" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTH5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01082"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1324"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050418">
        <property type="term" value="F:hydroxylamine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01914">
        <property type="entry name" value="HCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1270.20:FF:000001">
        <property type="entry name" value="Hydroxylamine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2030:FF:000001">
        <property type="entry name" value="Hydroxylamine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2030:FF:000002">
        <property type="entry name" value="Hydroxylamine reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00069">
        <property type="entry name" value="Hydroxylam_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010048">
        <property type="entry name" value="Hydroxylam_reduct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016100">
        <property type="entry name" value="Prismane_a-bundle"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01703">
        <property type="entry name" value="hybrid_clust"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003658">
        <property type="entry name" value="PRK05290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="HYDROXYLAMINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109:SF0">
        <property type="entry name" value="HYDROXYLAMINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000076">
        <property type="entry name" value="HCP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="Prismane protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="3"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="255"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="299"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via persulfide group" evidence="8">
        <location>
            <position position="389"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="442"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="477"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="479"/>
        </location>
        <ligand>
            <name>hybrid [4Fe-2O-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:60519"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Cysteine persulfide" evidence="8">
        <location>
            <position position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00051350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="535" mass="60444" checksum="589714DB1953BB98" modified="2013-06-26" version="1">MFCFQCQETAKNEGCTIRGVCGKDDKVANLQDLLIYLIKGLCLICEKSGYSDEKIDKFIVESLFATITNVNFDDSDIIERIKKCIELRENIKYNSENLPDCATWKPENDEDIIKKAYTHEVSVLAERDEDKRSLKELITYGIKGISAYLYHAMNLGYNDEEIHKFIKSTLAKLTEDLSVDELFNLAMETGKYAVKTLELLDKANTGTYGHPEITEVNIGVRNRPGILISGHDLKDLEQLLEQSKDKGVDIYTHCEMLPANYYPYFKKYEHFVGNYGGAWWRQREEFKKFNGPIIMTTNCLVPPDESYKDRLYVTGPVGYPGVKRIPEKDGVKDFSEVIEHAKKCNPPEPLEEGKIIGGFAHNQVIALADKIIKAINEGKIRKFVVMAGCDGRFKVREYYTEFAKKLPKDTVILTCGCAKYRFIKLDLGDIDGIPRVLDAGQCNDSYSLVKIALTLKDVLGLKDINELPIAYNISWYEQKAVAVLLALLYLGVKNIILGPTLPAFLSPNVAKVLVEKFGISQISTVEEDMKKLGII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTZ0</accession>
    <name>N6UTZ0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">(NiFe)-hydrogenase-3-type complex Eha, EhaM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05768</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592813.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTZ0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01252"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1124"/>
    <dbReference type="OrthoDB" id="59507at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3070.10">
        <property type="entry name" value="EhaM-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036606">
        <property type="entry name" value="EhaM-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012056">
        <property type="entry name" value="NiFe_EhaM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09218">
        <property type="entry name" value="EhaM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101332">
        <property type="entry name" value="Hypothetical protein MTH393"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="138" mass="16203" checksum="3DFA3E7788BD239A" modified="2013-06-26" version="1">MDVDRKYLEDSIKQKINIIKDNRFLMDNLFLPLAKKLDMDVDEIIEIFLKKVDFASVYELSAYVEQAKMGCLGRKVDIDLGLCYLADFFKLIDRKKADLIRKKVVELHLLYNKPYKDALNVGKKLIIKLLREKNEKSC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU88</accession>
    <name>N6UU88_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR system Cms endoribonuclease Csm3</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">CRISPR type III A-associated RAMP protein Csm3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_05269</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated Csm3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000035">
        <property type="protein sequence ID" value="ENN95914.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU88"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00741"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1029"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013412">
        <property type="entry name" value="CRISPR-assoc_RAMP_Csm3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052216">
        <property type="entry name" value="CRISPR_Csm3_endoribonuclease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02582">
        <property type="entry name" value="cas7_TM1809"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35579">
        <property type="entry name" value="CRISPR SYSTEM CMS ENDORIBONUCLEASE CSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35579:SF3">
        <property type="entry name" value="CRISPR SYSTEM CMS ENDORIBONUCLEASE CSM3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="4" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="9">
        <location>
            <begin position="32"/>
            <end position="212"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033183"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95914.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="219" mass="24517" checksum="CAF99F3365E74B9A" modified="2013-06-26" version="1">MVFGQTMNQNNNEQGNNEIYTLKGKVIISGVIELLTGLHIGGLRETLKIGGTDNPVIKDAFGNILIPGSSLKGKIRCLLEKKEGKYTNDNRGYLPCSCGDCNICKLFGPHNSKEIKEPRRVIFRDAYIEVEEGKEIHDYLEIKVENTIDRLKGTTITGGVRNTERVVAGSKFKFEIVFNVYKENDKELLKELITGMKLLEDDYLGGSGSRGYVKLNLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUG8</accession>
    <name>N6UUG8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Fibrillarin-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05060</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95984.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592141.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUG8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00477"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.992"/>
    <dbReference type="OrthoDB" id="114142at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016760">
        <property type="entry name" value="HcgG-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03958">
        <property type="entry name" value="monoFe_hyd_HmdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10113">
        <property type="entry name" value="Fibrillarin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019375">
        <property type="entry name" value="UCP019375"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95984.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="499" mass="56556" checksum="60ABCBF2190D37E6" modified="2013-06-26" version="1">MRDLIKESINNLDAALELRKIVLNKIRNRKLKESDIIEILDAIDDLSLEEIIKLGSNFRKFPLGCDLVDIAIGPCASNLSMLELLENCILADYIGYPIHICAYAIADIAEKEGIETIELFKKIIENVEVPIDIDHFGQYGPMRFPKEITHCYGECYFKGYFRGCPRKRIHKRLIEKEKKERFVEDYIRLASTVCVNVVEEQGAEEHAAPIEEMEIVANLAKKYGKGLEGIFHVGDGYDDLIEGINACLKLDVDVFVIEGACFNRAKDKLKAFAKAVAISRILVKGGVVATNGAYENECRIALRAGLNTIITGFPYNHHGYMCGYSPGTAKRGNFGLRRVMRIIKEEIKDLNVKLADKNINKAIAMGNNFLKDKIYPYTLGSFFLGDAHWRAVKESKLMKYKPEKTIDDIKEDKLGLIGGRYIAWAIAEKAEEVYISDRDPWVEKATVKVLNENGINAYPCNGDDREALRYKGYITTFIPELALKIYKKLKEYNNIELLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUT1</accession>
    <name>N6UUT1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NurA domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04508</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96099.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591832.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUT1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00623"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.884"/>
    <dbReference type="OrthoDB" id="33831at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018977">
        <property type="entry name" value="NurA_domain"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09376">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00933">
        <property type="entry name" value="NurA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NurA" evidence="1">
        <location>
            <begin position="41"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96099.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="357" mass="41890" checksum="78CD813A5C6C7018" modified="2013-06-26" version="1">MIEYLIKNKEAIKKRIKEIANNSYNIHEFWNPYEFDNPKNLTFSAGDGSYNHLDYLSFSFYAVGAVGLLHKVGGKIKRVKESYDFYIEHPLDITERLRLYMLILELKLAYYLLKNYNIDYYLFDGSLFSLLISAKTAIEKFGKDDFEKFYDKYKEELDNEIHKSLENDEIVINSKIYNLDREKKVLLEALEYILVLNKIINEFKNKIIGISKTSRINIYFNANIPDIAIFTKYTNDEGYSKPINFFETLAESYGEGYKQLSGVMKSICFIRKFGAKLNNAYIQFVRLERNKAVLGITSFDKIDLKVLSSLKAISIDGYPYVLKKAHNMVKITNKDMRYFAKFLNIEEPIARYMVERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUW3</accession>
    <name>N6UUW3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coenzyme F420-reducing hydrogenase subunit delta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_03758</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96144.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591338.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUW3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00384"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.734"/>
    <dbReference type="OrthoDB" id="85598at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1450:FF:000005">
        <property type="entry name" value="Hydrogenase maturation protease HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="entry name" value="HybD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004411">
        <property type="entry name" value="Pept_A31_F420-red_hyd_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00130">
        <property type="entry name" value="frhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="HYDROGENASE 1 MATURATION PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302:SF1">
        <property type="entry name" value="HYDROGENASE 2 MATURATION PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="HybD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96144.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="164" mass="18472" checksum="148DE0896822F1C9" modified="2013-06-26" version="1">MDLNPSYLQKEILIIGCGNILFADDGFAYHVIEKLKEKINDDRIAILDAGCAASHVLSLIDEESKVKKVLLVDVIDYGLKPGELKILSLEELPDVRYDRIDIHNYPLAEKLKELSKKGIEVKVIGCQAKYISEPDVHIGLSKEVEDAIDRACDLVIEEVKKWLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUY7</accession>
    <name>N6UUY7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Molybdopterin-guanine dinucleotide biosynthesis protein MobB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_03938</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96174.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591395.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUY7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00417"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.772"/>
    <dbReference type="OrthoDB" id="9014at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007202">
        <property type="entry name" value="4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052539">
        <property type="entry name" value="MGD_biosynthesis_adapter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004435">
        <property type="entry name" value="MobB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00176">
        <property type="entry name" value="mobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40072:SF1">
        <property type="entry name" value="MOLYBDOPTERIN-GUANINE DINUCLEOTIDE BIOSYNTHESIS ADAPTER PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40072">
        <property type="entry name" value="MOLYBDOPTERIN-GUANINE DINUCLEOTIDE BIOSYNTHESIS ADAPTER PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04060">
        <property type="entry name" value="FeS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03205">
        <property type="entry name" value="MobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51656">
        <property type="entry name" value="4FE4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S" evidence="5">
        <location>
            <begin position="130"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96174.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="232" mass="26195" checksum="9065069C63B3368D" modified="2013-06-26" version="1">MRVIGVIGYKNSGKTKLIEDIISNWDKKIAVIKHTIDDIDLKGKDSYRFRDAGAKISIISSDKETVFFLNKLNLDEILGILMGYDIDFVIIEGFKDELKKLNIPKIVLLKNGDDKLIDEQTAYVIKGDYNIDEVIKIIEEKAIIPSMNLNCGHCGYNCETFVKALCKNEVKWDQCVLSNGIKIIVDNKIIPTVPFVSKIIGRSLIAMLECLKGVNKPKHVKIIIDTKKLKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV03</accession>
    <name>N6UV03_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">MTH865-like family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03558</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96194.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591240.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV03"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01391"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.694"/>
    <dbReference type="OrthoDB" id="59514at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.80">
        <property type="entry name" value="MTH865-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036825">
        <property type="entry name" value="MTH865-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024093">
        <property type="entry name" value="Uncharacterised_MTH865"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07747">
        <property type="entry name" value="MTH865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69025">
        <property type="entry name" value="Hypothetical protein MTH865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96194.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="88" mass="10109" checksum="0CA0EB5312F56491" modified="2013-06-26" version="1">MIRTNHPLYEALRDIEEFKLKLAEYFKDKDVFPIKSRVELANLLPCGISLKCGEVEASELVKLLDESDFPIKDLNDLVTKLSKKCPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV14</accession>
    <name>N6UV14_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">tRNA (guanine(26)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="7 8">2.1.1.216</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">tRNA 2,2-dimethylguanosine-26 methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA(guanine-26,N(2)-N(2)) methyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">tRNA(m(2,2)G26)dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trm1</name>
        <name evidence="10" type="ORF">J422_03618</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Dimethylates a single guanine residue at position 26 of a number of tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43140"/>
            <dbReference type="Rhea" id="RHEA-COMP:10359"/>
            <dbReference type="Rhea" id="RHEA-COMP:10360"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.216"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="8 9">Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.216" evidence="7 8"/>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96204.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591259.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV14"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01380"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.706"/>
    <dbReference type="OrthoDB" id="372177at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160104">
        <property type="term" value="F:tRNA (guanine(26)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002940">
        <property type="term" value="P:tRNA N2-guanine methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000272">
        <property type="entry name" value="tRNA (guanine(26)-N(2))-dimethyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.70">
        <property type="entry name" value="N2,N2-dimethylguanosine tRNA methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00290">
        <property type="entry name" value="tRNA_dimethyltr_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002905">
        <property type="entry name" value="Trm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022923">
        <property type="entry name" value="TRM1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042296">
        <property type="entry name" value="tRNA_met_Trm1_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00308">
        <property type="entry name" value="TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631">
        <property type="entry name" value="N 2 ,N 2 -DIMETHYLGUANOSINE TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10631:SF3">
        <property type="entry name" value="TRNA (GUANINE(26)-N(2))-DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02005">
        <property type="entry name" value="TRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51626">
        <property type="entry name" value="SAM_MT_TRM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 8 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8 9" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="1 8 9" id="KW-0820">tRNA-binding</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00039099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96204.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="372" mass="42178" checksum="E3F01514809777C5" modified="2013-06-26" version="1">MKVKEGKISFEIPDKLTVTRKDEVFYNPKMETCRDIAVAIIEGFLKLYCNNKIAYIADALAGTGVRGLRYAKEVDYNVKVYLNDINPKAYEKIKENAKLNGIDNIEIFNEDANIFLAKHYRFFNVVDIDPFGSPSPYIDQAIRSLITKNGLLCLTATDTAALCGRAKRACLRKYLAYPLLGKDCHEFALRILVGYAMRLATRYNLLLTPVFAHATDHYVRVYLKTDRGAKRADKAFEMLGYVKDINGIKIVKAFSEGYEKGYSGPLYIGNLYDGKLVEKAINIAKNKGFKERVIKILMRIRDESEISQIGCYDTHLIAKILKVSVPPMEYIVNSLKDLGYKAAITHYNPKGIKTNANIKTLMEVLLEYKMRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV33</accession>
    <name>N6UV33_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_03468</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000022">
        <property type="protein sequence ID" value="ENN96224.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591138.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV33"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01340"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.676"/>
    <dbReference type="OrthoDB" id="19148at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050833">
        <property type="entry name" value="Poly_Biosynth_Transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250:SF28">
        <property type="entry name" value="POLYSACCHARIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250">
        <property type="entry name" value="PST FAMILY PREDICTED COLANIC ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13440">
        <property type="entry name" value="Polysacc_synt_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="86"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="113"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="146"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="173"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="213"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="243"/>
            <end position="266"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="287"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="352"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="377"/>
            <end position="397"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96224.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="403" mass="46009" checksum="5A61A32D468E8096" modified="2013-06-26" version="1">MLLESLILTFFNFSSGFFNYLYQLFMGKLLTPEEYGVVASLLSIFLILGIFPRVINSSLTPQISKLKAKNRLNEINYIFLHYTKRFLILGVIITIIFLILSPLISNFLHIKNIYTIIILLNLPFIFINPINLAILQGLQRFTKLGFTNTLTSLAKFLFGILLVYLTFGVFGALIAILLSSIISYLVSLYFIRDILKLKPKEYFIKNFYKTSKLIFLIIFSYTTITTVDVILAKHYLSKYLAGIYSSVSTLGKIIFFLPGGIQAIILSKAIEYKEKNLNHLNLLKKSIIFLILLTLPILTSYIFFPELVIKVLFSKKYLLAKLYIGKYGLAMLLFSISGLIMNYLISLNVNRIAYPLILVLIFEILAFIIFPKTIDNFINILILTSLLSIVVQVPYIIKGGKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV91</accession>
    <name>N6UV91_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2304 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03211</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000019">
        <property type="protein sequence ID" value="ENN96279.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590895.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV91"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00319"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.630"/>
    <dbReference type="OrthoDB" id="78194at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019277">
        <property type="entry name" value="DUF2304"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10066">
        <property type="entry name" value="DUF2304"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="29"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="67"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96279.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="113" mass="13297" checksum="0C8F01A0866593A3" modified="2013-06-26" version="1">MLIQIFGILFCIFALIITLYRTKKRYMSIEKGVFWVIIWMIILLLLINPNLMSKIADILGVGRGVDAIVYTTLAILLYLVYKLFERTERLEREITKLVREIAIKDRYEPKKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVB9</accession>
    <name>N6UVB9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 11">7-cyano-7-deazaguanine synthase</fullName>
            <ecNumber evidence="9 11">6.3.4.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">7-cyano-7-carbaguanine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Archaeosine biosynthesis protein QueC</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">PreQ(0) synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">queC</name>
        <name evidence="12" type="ORF">J422_03054</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 11">Catalyzes the ATP-dependent conversion of 7-carboxy-7-deazaguanine (CDG) to 7-cyano-7-deazaguanine (preQ(0)).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>7-carboxy-7-carbaguanine + NH4(+) + 2 ATP = 7-cyano-7-carbaguanine + 2 AMP + 2 diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:27982"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8 11">Belongs to the QueC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.20" evidence="9 11"/>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96304.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590755.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVB9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00158"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.599"/>
    <dbReference type="OrthoDB" id="6532at2157"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01995">
        <property type="entry name" value="QueC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01633">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018317">
        <property type="entry name" value="QueC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00364">
        <property type="entry name" value="7-cyano-7-deazaguanine synthase QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42914:SF1">
        <property type="entry name" value="7-CYANO-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06508">
        <property type="entry name" value="QueC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006293">
        <property type="entry name" value="ExsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="7"/>
            <end position="17"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00037768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00039149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96304.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="232" mass="26500" checksum="A5856B9270B3DA97" modified="2013-06-26" version="1">MKAVSILSGGLDSTVATMIAKEEYDEVTAITFNYGQKAFKREVSAAKKICEILGIKHVIINLEFVKLFNKGSLITDEELPKIKIEELNTEKAFETMKAVWVPARNLIFLSIASGFAEAYEMDRVIIGINKEEGQTFPDNRIEFIEAFNNALKYGTLKDIKVEAPLYDKSKEEIVKIGHELEKKYGEVLRYTYSCYKDNGKDFLHCGECESCMRRKRAFMLAKVEDKTEYLTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVG3</accession>
    <name>N6UVG3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9 10">Cell division protein FtsZ</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ftsZ</name>
        <name evidence="13" type="ORF">J422_02649</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Essential cell division protein that forms a contractile ring structure (Z ring) at the future cell division site. The regulation of the ring assembly controls the timing and the location of cell division. One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. Binds GTP and shows GTPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer. Polymerizes to form a dynamic ring structure in a strictly GTP-dependent manner. Interacts directly with several other division proteins.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="8">Assembles at midcell at the inner surface of the cytoplasmic membrane.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the FtsZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96344.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590567.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVG3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00082"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.519"/>
    <dbReference type="OrthoDB" id="371908at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032153">
        <property type="term" value="C:cell division site"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043093">
        <property type="term" value="P:FtsZ-dependent cytokinesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051258">
        <property type="term" value="P:protein polymerization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02201">
        <property type="entry name" value="FtsZ_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.20:FF:000008">
        <property type="entry name" value="Cell division protein FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1440:FF:000023">
        <property type="entry name" value="Cell division protein FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1110">
        <property type="entry name" value="Tubulin, GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.20">
        <property type="entry name" value="Tubulin/FtsZ, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1440">
        <property type="entry name" value="Tubulin/FtsZ, GTPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00909">
        <property type="entry name" value="FtsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000158">
        <property type="entry name" value="Cell_div_FtsZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020805">
        <property type="entry name" value="Cell_div_FtsZ_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045061">
        <property type="entry name" value="FtsZ/CetZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024757">
        <property type="entry name" value="FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008280">
        <property type="entry name" value="Tub_FtsZ_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037103">
        <property type="entry name" value="Tubulin/FtsZ-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018316">
        <property type="entry name" value="Tubulin/FtsZ_2-layer-sand-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036525">
        <property type="entry name" value="Tubulin/FtsZ_GTPase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003008">
        <property type="entry name" value="Tubulin_FtsZ_GTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00065">
        <property type="entry name" value="ftsZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314">
        <property type="entry name" value="CELL DIVISION PROTEIN FTSZ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30314:SF3">
        <property type="entry name" value="MITOCHONDRIAL DIVISION PROTEIN FSZA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12327">
        <property type="entry name" value="FtsZ_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00091">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00423">
        <property type="entry name" value="CELLDVISFTSZ"/>
    </dbReference>
    <dbReference type="SMART" id="SM00864">
        <property type="entry name" value="Tubulin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00865">
        <property type="entry name" value="Tubulin_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55307">
        <property type="entry name" value="Tubulin C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52490">
        <property type="entry name" value="Tubulin nucleotide-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01134">
        <property type="entry name" value="FTSZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01135">
        <property type="entry name" value="FTSZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 8 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 8 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8 10" id="KW-0717">Septation</keyword>
    <feature type="domain" description="Tubulin/FtsZ GTPase" evidence="11">
        <location>
            <begin position="38"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="Tubulin/FtsZ 2-layer sandwich" evidence="12">
        <location>
            <begin position="231"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="46"/>
            <end position="50"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="133"/>
            <end position="135"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR00065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96344.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="368" mass="39514" checksum="5A1B49F613DF30FC" modified="2013-06-26" version="1">MKFLEKVLKEEGIEEKEDFEMSPEDQELLEILEQTKARITVVGCGGAGNNTITRLYTEGIEGAKTVAINTDAQQLLRTKADKKILIGKKLTRGLGAGGNPKIGEEAAKESAEEIKAALQDSDMVFITCGLGGGTGTGSSPVVAEIAKKLGALTVAVVTLPFLMEGKVRMRNAIQGLEKLKQFTDTLVIIPNEKLFDIVPNMPIKLAFKVADEVLINSVKGLVELITKDGLINVDFADVKAVMSNGGMAMIGLGDSDSENRAKEAVQMALNSPLLEVDIEGAKGALIHVMGPEDLTLEEAKQVVSTVSSKLDPEATIIWGATIDENLENTLKVLLVVTGVKPTIDFEKGIIRKTHKKDKLKKELGIPDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVN9</accession>
    <name>N6UVN9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Adenylyl cyclase CyaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02499</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96409.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590451.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVN9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00772"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.489"/>
    <dbReference type="OrthoDB" id="46040at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07890">
        <property type="entry name" value="CYTH-like_AC_IV-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.320.10">
        <property type="entry name" value="Hypothetical Protein Pfu-838710-001"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008173">
        <property type="entry name" value="Adenylyl_cyclase_CyaB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033469">
        <property type="entry name" value="CYTH-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023577">
        <property type="entry name" value="CYTH_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00318">
        <property type="entry name" value="cyaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028:SF2">
        <property type="entry name" value="CYTH DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21028">
        <property type="entry name" value="SI:CH211-156B7.4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01928">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01118">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55154">
        <property type="entry name" value="CYTH-like phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51707">
        <property type="entry name" value="CYTH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CYTH" evidence="2">
        <location>
            <begin position="1"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96409.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="186" mass="22630" checksum="2B5329310F15400A" modified="2013-06-26" version="1">MIEVEIKVRIKNIDEIKKKLEKLGFKFKEKKFQEDIYFNHPQRDFRKTDEALRIRNDNNTYYLTYKGPKIDNISKTREEIEVRIEDKEKMRKILKKLGFIEVKPIKKTREIYKKNDITACIDNIFGLGYFLELEKPVNSFDEKDKALDELLNILKALNIKRERIIKKSYLEMRESYEKRKQKNKFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVV8</accession>
    <name>N6UVV8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Methyl-coenzyme M reductase subunit beta</fullName>
            <ecNumber evidence="9">2.8.4.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Coenzyme-B sulfoethylthiotransferase beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_02205</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>coenzyme B + methyl-coenzyme M = methane + coenzyme M-coenzyme B heterodisulfide</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyl-coenzyme M reductase beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96464.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590268.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVV8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01189"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.433"/>
    <dbReference type="OrthoDB" id="52873at2157"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="entry name" value="Methyl-coenzyme M reductase, alpha/beta subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003179">
        <property type="entry name" value="Me_CoM_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022679">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022680">
        <property type="entry name" value="Me_CoM_Rdtase_bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03257">
        <property type="entry name" value="met_CoM_red_bet"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02241">
        <property type="entry name" value="MCR_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02783">
        <property type="entry name" value="MCR_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000263">
        <property type="entry name" value="Meth_CoM_rd_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="Methyl-coenzyme M reductase alpha and beta chain C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="Methyl-coenzyme M reductase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyl-coenzyme M reductase beta subunit N-terminal" evidence="11">
        <location>
            <begin position="6"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-coenzyme M reductase beta subunit C-terminal" evidence="10">
        <location>
            <begin position="189"/>
            <end position="437"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010675"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02783"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96464.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="444" mass="47590" checksum="1C76AEAFF394AE36" modified="2013-06-26" version="1">MMKYKDTINLYDAKGNLVEENVPLEAVSPLYNPTIQKLIKDVKRTVAVNLAGIENALRNGTVGGKGNKLPGRELDLPIVENAETIAEYVERVIRVSEDDDTNIRLINDGKQMAVQMPTKRLDIAAEYTTSILVTAQALKEAIIRTFDVDIFDAPMVHAAILGGYPHNVVMAGANVAGLLGNPLTLEGPGYALRNIMVNHFVACTKKNVMNAVAFASIMEQTAMFEMGDAVGLFERLHLLGLAYQGLNADNLVIDLVKENGKNGTVGTVVASVVERALEDGVIKEDRTLPSGFKLYKPVDVAKWNAYAAAGLVAAVIVNVGAARAAQGVASTILYYNDILEYETGLPGVDFGRAEGTAVGFSFFSHSIYGGGGPGVFNGNHIVTRHSKGFAIPPVAAAMCLDAGTQMFSPERTSALIGTVFSAIDEFREPLKYVIEGALQVKDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVY5</accession>
    <name>N6UVY5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Hydrogenase maturation protease HycI</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_01680</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96489.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590094.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVY5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00187"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.330"/>
    <dbReference type="OrthoDB" id="44145at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="entry name" value="HybD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004420">
        <property type="entry name" value="Pept_A31_hyd_mat_HycI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00142">
        <property type="entry name" value="hycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="HYDROGENASE 1 MATURATION PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302:SF1">
        <property type="entry name" value="HYDROGENASE 2 MATURATION PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="HybD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96489.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="147" mass="16890" checksum="4A5DB9EDE520B649" modified="2013-06-26" version="1">MKVVIGIGNELRGDDAVGCYIAKRLIEYFNGEGDIFKKDNLCIINARTSLEAYTDIIKELKPKHIIIVDCALINEDYRIIKLDEIDNLLLSSHNLPISFIIKYLKNFIDFDLTIIGIRPRVIDFCDMSNETKKIAEKVLKEIIKMVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6UW56</accession>
    <name>N6UW56_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">2-hydroxyglutaryl-CoA dehydratase subunit D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FldB/FldC dehydratase alpha/beta subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96549.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UW56"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00251"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.398"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010327">
        <property type="entry name" value="FldB/FldC_alpha/beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047678">
        <property type="entry name" value="YjiM-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040772">
        <property type="entry name" value="double_cubane"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30548">
        <property type="entry name" value="2-HYDROXYGLUTARYL-COA DEHYDRATASE, D-COMPONENT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30548:SF1">
        <property type="entry name" value="DEHYDRATASE SUBUNIT MJ0007-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06050">
        <property type="entry name" value="HGD-D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="202"/>
            <end position="229"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96549.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="372" mass="42741" checksum="5C8BBA521EBA25E2" modified="2013-06-26" version="1">MELNAIKKLMEKFANRKEQLYKQKEEGKKVFGMFCAFVPIELILAADAIPVGLCGGKNDTVPIAEEDLPRNLCPLIKSSYGFKKAKSCPYFEAADVIIGETTCDGKKKMFELLERMVKMHVMLLPYMKTEKAKELWVEEVKKLKELVEKETGNKIDEEKLKESVEKVNKMRKLFYKLYELRANKPTPIKGLDALKLFQFAYLLDIDDTIEILEELVKELEERVKKGEGYEGKRILISGCPMVAGNTKIVELIEEVGGVVVGEESCTGTRFFENLVEEPTIEGIAERYLKIPCACMFHNDERIENIKRLVKELNADGVVYYTLQYCHTFNVEGTRVEEELKKEGIPVIRIETDYSESDKEQLKTRLEAFIEMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6UW62</accession>
    <name>N6UW62_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carbohydrate kinase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02055</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590195.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UW62"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00256"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.403"/>
    <dbReference type="OrthoDB" id="15148at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="YjeF C-terminal" evidence="1">
        <location>
            <begin position="106"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="277" mass="30407" checksum="B6A1A33AA05F3D78" modified="2013-06-26" version="1">MIIAGTIPIEGLELIKGKPILKGNKIIINNKEFPISMGTCALIGAVLKTLEYFDENNIHVITAGDIGKGDGSLKIYDALKEVDDDLLIIHYIKPKISYIKEIDFSPKIIADAGGMYAAKAANIGDKFHLFLPDVGELAFLADEKASHPAYVRGFISEVDNKEVPELIRRAYGKSKMPKYMVVKGEKDYIVEDGNIAEIIDSPKIEAMECIGGTGDTLTGIISSLINCGYKTKDACVLGCKINRILGEISNVKPNTQINELIYNIPKVLKFILNKELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWG3</accession>
    <name>N6UWG3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Roadblock/LC7 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01191</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589904.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWG3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00806"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.233"/>
    <dbReference type="OrthoDB" id="92542at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0060090">
        <property type="term" value="F:molecular adaptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032008">
        <property type="term" value="P:positive regulation of TOR signaling"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.30">
        <property type="entry name" value="Dynein light chain 2a, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037587">
        <property type="entry name" value="LAMTOR2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004942">
        <property type="entry name" value="Roadblock/LAMTOR2_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13323">
        <property type="entry name" value="LATE ENDOSOMAL/LYSOSOMAL MP1 INTERACTING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03259">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00960">
        <property type="entry name" value="Robl_LC7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103196">
        <property type="entry name" value="Roadblock/LC7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Roadblock/LAMTOR2" evidence="1">
        <location>
            <begin position="2"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="115" mass="12215" checksum="D795C0F26CAAFF03" modified="2013-06-26" version="1">MIDRVLLELNKTEGIKGSLVVGKDGLIIASQLSGNIDAELVGAMVSAAYGAAERTASEIGIGKLEQIMVESEHGKILAYDANEAILAILTEPKVNLGLVRIVAKKARDKIKTMLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWH2</accession>
    <name>N6UWH2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CODH nickel-insertion accessory protein CooC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01011</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000006">
        <property type="protein sequence ID" value="ENN96674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589870.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWH2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01016"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.197"/>
    <dbReference type="OrthoDB" id="31168at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014433">
        <property type="entry name" value="CooC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF3">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005647">
        <property type="entry name" value="CooC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="1">
        <location>
            <begin position="7"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="233" mass="25839" checksum="E7665B2D5B96A6CC" modified="2013-06-26" version="1">MKISICGKGGCGKSTVTALLAKELSKNKKVLVIDGDESNISLHRLLGLEKPKDIIDIIGGRKVLKKNMNNISEILKDIDVDSIASKDGNLMLISVGKIRDFGEGCACPMGVLLKELIKNLDYDYVLIDTEAGIEHFGRGVEENVDVILVVIDPTYESIRLANEIERMAKNMGKKVYFIINKVDDNIKDMIMDKINKDRVIAIIPNKREILEKGLLGESLDSIEEIKDIVKTIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWI9</accession>
    <name>N6UWI9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">3-isopropylmalate dehydratase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00946</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589859.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWI9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00585"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.186"/>
    <dbReference type="OrthoDB" id="31247at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10900">
        <property type="entry name" value="PAC-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004426">
        <property type="entry name" value="MJ1210-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019151">
        <property type="entry name" value="Proteasome_assmbl_chaperone_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038389">
        <property type="entry name" value="PSMG2_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00162">
        <property type="entry name" value="proteasome assembly chaperone family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610:SF7">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35610">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09754">
        <property type="entry name" value="PAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159659">
        <property type="entry name" value="Cgl1923-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="198"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="253" mass="28253" checksum="5F4D7B496A47FCA4" modified="2013-06-26" version="1">MIKFIKRKIKDIKLENALLIEGLPGIGHVGRLAAEHLVHEFNGEKFLELYCYDFPPQVLVKDDGTIEYMKADFYAIKEPKHMIVVLGNTQALTPVGQYHLAEEIVKLGIEHGANFVYTFGGFGIGKLVKEAKVYGATTSKELAEKLKEHGVIFRNDGGGIVGAAGLMLLFADLNNIPGICLMGETPGYLIDPNAAKAVLEKFSKLENIEINMEELEKRAKSMEQFIERIKKFEEETLKALQKPPSDEDLRYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWL1</accession>
    <name>N6UWL1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Molybdenum ABC transporter, periplasmic molybdate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_00726</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589819.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWL1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00891"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.140"/>
    <dbReference type="OrthoDB" id="15033at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030973">
        <property type="term" value="F:molybdate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13517">
        <property type="entry name" value="PBP2_ModA3_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000035">
        <property type="entry name" value="Molybdate ABC transporter substrate-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005950">
        <property type="entry name" value="ModA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050682">
        <property type="entry name" value="ModA/WtpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001638">
        <property type="entry name" value="Solute-binding_3/MltF_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01256">
        <property type="entry name" value="modA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30632">
        <property type="entry name" value="MOLYBDATE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30632:SF0">
        <property type="entry name" value="SULFATE-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13531">
        <property type="entry name" value="SBP_bac_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004846">
        <property type="entry name" value="ModA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00062">
        <property type="entry name" value="PBPb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0732">Signal</keyword>
    <feature type="domain" description="Solute-binding protein family 3/N-terminal" evidence="4">
        <location>
            <begin position="39"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="267" mass="29564" checksum="2EFBE1D0C192260D" modified="2013-06-26" version="1">MKKFTILILTVGFLLITLCGCTEKTHDSQNSAKIYEGHSIIVLCGAGLIKPMEELKRNFENKTGAKVNVHYGGSAELFGILKTTGGDVFIPGAYKYTKDAMDIGLILNDTVKNITYHIPVIIVPKGNPKNIKDLNDLTKPGVRIAIGDPKSSAIGKVSVKIFEKNKIWEKVKPNIVVYAPTVNQLLIYVATKQVDAAIIWEDMTTWSQAKGKIEVIEIPKDKNIIKTIPTAVTTKAKDLEVAKAFNNYISSDEAKTIWEKWGFKPCR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWN0</accession>
    <name>N6UWN0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ATPase AAA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_00871</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWN0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00860"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.172"/>
    <dbReference type="OrthoDB" id="65289at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051667">
        <property type="entry name" value="Archaeal_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049081">
        <property type="entry name" value="MJ1010-like_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096:SF1">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096">
        <property type="entry name" value="YALI0E33429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21690">
        <property type="entry name" value="MJ1010-like_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Uncharacterized ATP-binding protein MJ1010-like C-terminal" evidence="3">
        <location>
            <begin position="40"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="47"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF21690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="128" mass="15263" checksum="B62CB09C505E5A76" modified="2013-06-26" version="1" fragment="single">ENILNKKLSNEEKELIYSYVGGKPVLIIKVINKMRTEELDEILNFMLNDTKQRLKYLLEDIREENEELYKEIIKALSLFKENHEVEDITIDKKVREFLVKRNILFLDTIKGVIKPQSFLIWNAIKILI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWQ9</accession>
    <name>N6UWQ9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11 12">Histidinol dehydrogenase</fullName>
            <shortName evidence="11 12">HDH</shortName>
            <ecNumber evidence="4 11 12">1.1.1.23</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 18" type="primary">hisD</name>
        <name evidence="18" type="ORF">J422_00416</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11 12">Catalyzes the sequential NAD-dependent oxidations of L-histidinol to L-histidinaldehyde and then to L-histidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11 12">
            <text>L-histidinol + 2 NAD(+) + H2O = L-histidine + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:20641"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57595"/>
            <dbReference type="ChEBI" id="CHEBI:57699"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 16">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11 16">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 9/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 12 17">Belongs to the histidinol dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.23" evidence="4 11 12"/>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96779.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589765.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWQ9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00697"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.80"/>
    <dbReference type="OrthoDB" id="36308at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00014"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004399">
        <property type="term" value="F:histidinol dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06572">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000001">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1980:FF:000026">
        <property type="entry name" value="Histidinol dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.1300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01024">
        <property type="entry name" value="HisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022695">
        <property type="entry name" value="Histidinol_DH_monofunct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012131">
        <property type="entry name" value="Hstdl_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00069">
        <property type="entry name" value="hisD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256:SF2">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS TRIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21256">
        <property type="entry name" value="HISTIDINOL DEHYDROGENASE HDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00815">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000099">
        <property type="entry name" value="Histidinol_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00083">
        <property type="entry name" value="HOLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11 12" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="6 11 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11 12 14" id="KW-0520">NAD</keyword>
    <keyword evidence="8 11 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11 16" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="11 13">
        <location>
            <position position="323"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="11 13">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 15">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 16">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 15">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 16">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 15">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 15">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 16">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 15">
        <location>
            <position position="357"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 15">
        <location>
            <position position="411"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 16">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 15">
        <location>
            <position position="416"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049489"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR000099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000099-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU004175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ENN96779.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="424" mass="47185" checksum="7BB08985134B351D" modified="2013-06-26" version="1">MIVKDIKTLSLDERKKILERNKINFENILLDVLKILKDVRERGDEAIKYYTKKFDNVDIDNFIVSNEEIEEAYNRVDYKIIDALERAKENIYYFHKKQLKQIKELRIEKEGMVLGQVIRAVEKVGCYVPGGKAFYPSTVLMTTIPAKVAGCKKIFITSPPTPEGKGNCATLVAGDIVGVDRIYKIGGVQAIGALAYGTESVEKVDLIVGPGNIYVTLAKKLVYGDVNIDLLAGPSEVLIIADDSANVNYIAIDVIAQAEHDPNAHCVVLTTSKEKAYEIKNTIEKELKNIKRKEIVEKSLKNAAILYGELNELINFANEYAPEHLEIITKDPESVLEKINNAGSIFLGEYSPVPVGDYASGTNHVLPTSGFARAASGLSVETFLKKITYQKLSREGLEELKDTIITLAEAEGLYNHAEAIRRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWW3</accession>
    <name>N6UWW3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Putative cobalt transport protein CbiM</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Energy-coupling factor transporter probable substrate-capture protein CbiM</fullName>
            <shortName evidence="12">ECF transporter S component CbiM</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cbiM</name>
        <name evidence="13" type="ORF">J422_00265</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Part of the energy-coupling factor (ECF) transporter complex CbiMNOQ involved in cobalt import.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Forms an energy-coupling factor (ECF) transporter complex composed of an ATP-binding protein (A component, CbiO), a transmembrane protein (T component, CbiQ) and 2 possible substrate-capture proteins (S components, CbiM and CbiN) of unknown stoichimetry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Cell membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the CbiM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589740.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWW3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01110"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.53"/>
    <dbReference type="OrthoDB" id="30946at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1760.20:FF:000001">
        <property type="entry name" value="Cobalt transport protein CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1760.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01462">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018024">
        <property type="entry name" value="CbiM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002751">
        <property type="entry name" value="CbiM/NikMN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00123">
        <property type="entry name" value="cbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006184">
        <property type="entry name" value="PRK08319.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43627">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43627:SF1">
        <property type="entry name" value="COBALT TRANSPORT PROTEIN CBIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01891">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 12" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0170">Cobalt</keyword>
    <keyword evidence="3 12" id="KW-0171">Cobalt transport</keyword>
    <keyword evidence="9 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="10 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="4 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="41"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="78"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="105"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="139"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="169"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="230" mass="24661" checksum="1D0A4604B59B642B" modified="2013-06-26" version="1">MHIMEGFLPPMWCAFWYILSGIVWIYGVMQLKKLFDEMPEAKPLVAIAGAYMFILSSLKMPSVTGSCSHPCGNGLGAVLFGPSVTAVLASIVLLFQALFLAHGGITTLGANVFSMGIVGPFVGWLIYKALKGKMDSTYVVGLAAMFADWATYATTSLQLTLAFPGKDPIATFTAFATVFAITQIPLAIAEGLLTMIIWDYLKKLKPDLLLKLGVIDESEINVNVAPAGGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6UZZ5</accession>
    <name>N6UZZ5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">3-isopropylmalate dehydratase small subunit</fullName>
            <ecNumber evidence="7">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Alpha-IPM isomerase</fullName>
            <shortName evidence="7">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">leuD</name>
        <name evidence="9" type="ORF">J422_06942</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 7">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the LeuD family. LeuD type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000065">
        <property type="protein sequence ID" value="ENN95603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594632.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UZZ5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00855"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1345"/>
    <dbReference type="OrthoDB" id="6505at2157"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.19.10:FF:000007">
        <property type="entry name" value="Isopropylmalate/citramalate isomerase small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01032">
        <property type="entry name" value="LeuD_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050075">
        <property type="entry name" value="LeuD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011827">
        <property type="entry name" value="LeuD_type2/HacB/DmdB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02087">
        <property type="entry name" value="LEUD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43345:SF9">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43345">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="8">
        <location>
            <begin position="40"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="166" mass="18453" checksum="908BF7EEBB9F5BB4" modified="2013-06-26" version="1">MIRGRVWKFGDNVDTDAIMPARYLVYTEPEELAKYVMTGIDEDFPKKVKKGDIIVAGKNFGCGSSREHAPLGLKGAGISCIIAKSFARIFYRNAINLGLPLIECKDVDKINEGDILEVDLKSGVIRNVTTGEVLYGNKLPDFMMEILEAGGLMNYLKKRLANEKSY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6V026</accession>
    <name>N6V026_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06738</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000058">
        <property type="protein sequence ID" value="ENN95643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593989.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V026"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01358"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1311"/>
    <dbReference type="OrthoDB" id="11867at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.660.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43734:SF1">
        <property type="entry name" value="PHYTOENE DESATURASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13450">
        <property type="entry name" value="NAD_binding_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="394" mass="43747" checksum="9B17C714D41E2E25" modified="2013-06-26" version="1">MEIGIVGAGLGGLLAGAILSYNHKITIFEKLPFLGGRFTNLNYDGFQLTTGALHMIPHGSRGYLAKALKMAKADVKIVNSKPDGTFLINGREYLYKDLFDLLGVKDKIKALKKATNLKLGIVDYNQPFGEFLEDVDLALMIGKAFTGWALSLDVYEVTMKEIINIANNYYKFKGPGVPIGGCKAVIDELVKIIERNGGNIIKEYEVKKIEVEDKAYIDDREFDIVISNISPKETQKMCNIKFLDKKIEPSKGIKISIASKKGIIKHNGVLFTPECERINGLNQVTNVDKSLAPEGYHLIMTHQAQISNNVKKEIDLGLEDIDKLFKNIDYKILHIQSYRDDFPVNHAKNGHDVGNIINDRLYLVGDGAKGGDMEVEGIAIGVLKVVNHINQLNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6V086</accession>
    <name>N6V086_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06199</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593333.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V086"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00298"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1207"/>
    <dbReference type="OrthoDB" id="380159at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="63" mass="7244" checksum="29E342C031517D52" modified="2013-06-26" version="1">MGKLKIYRVDATEFNKNVGDFIKKGEFLGYFKNKKVIANENGYICGISFDAENHCLNIIIEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0T6</accession>
    <name>N6V0T6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 19">Glutamine synthetase</fullName>
            <ecNumber evidence="4 19">6.3.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="22" type="ORF">J422_05234</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 19">
            <text>L-glutamate + NH4(+) + ATP = L-glutamine + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16169"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="15">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Oligomer of 12 subunits arranged in the form of two hexagons.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 18">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 16 17">Belongs to the glutamine synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.2" evidence="4 19"/>
    <dbReference type="EMBL" id="APMM01000034">
        <property type="protein sequence ID" value="ENN95923.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592265.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0T6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00303"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1022"/>
    <dbReference type="OrthoDB" id="36124at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004356">
        <property type="term" value="F:glutamine synthetase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006542">
        <property type="term" value="P:glutamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.590.10:FF:000003">
        <property type="entry name" value="Glutamine synthetase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.70">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.590.10">
        <property type="entry name" value="Glutamine synthetase/guanido kinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008147">
        <property type="entry name" value="Gln_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036651">
        <property type="entry name" value="Gln_synt_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008146">
        <property type="entry name" value="Gln_synth_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027303">
        <property type="entry name" value="Gln_synth_gly_rich_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004809">
        <property type="entry name" value="Gln_synth_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027302">
        <property type="entry name" value="Gln_synth_N_conserv_site"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00653">
        <property type="entry name" value="GlnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785">
        <property type="entry name" value="GAMMA-GLUTAMYLPUTRESCINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43785:SF12">
        <property type="entry name" value="TYPE-1 GLUTAMINE SYNTHETASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00120">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03951">
        <property type="entry name" value="Gln-synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01230">
        <property type="entry name" value="Gln-synt_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54368">
        <property type="entry name" value="Glutamine synthetase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00180">
        <property type="entry name" value="GLNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00181">
        <property type="entry name" value="GLNA_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51986">
        <property type="entry name" value="GS_BETA_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51987">
        <property type="entry name" value="GS_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 18" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 19" id="KW-0436">Ligase</keyword>
    <keyword evidence="11 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 14 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="GS beta-grasp" evidence="20">
        <location>
            <begin position="13"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="GS catalytic" evidence="21">
        <location>
            <begin position="112"/>
            <end position="448"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="138"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="203"/>
            <end position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="244"/>
            <end position="245"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="251"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="309"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="340"/>
        </location>
        <ligand>
            <name>L-glutamate</name>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR604809-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU000384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="RuleBase" id="RU000385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU004356"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ENN95923.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="448" mass="51155" checksum="193D75C9834DF3B6" modified="2013-06-26" version="1">MDVDKAIEYIRKYNVKFIRFQFVDILGFPKNVAYPVKAGEKGIEELKEIFENGVWFDGSSIKGFVGIEESDMLLKPDLNTISVLPWRPEEKSVARVICDVYRNEKHPFEGDPRSRLRILLDELKKEMNGEFFVGPEPEFFLLRRDPHNPHRWIPADSGGYFDVEPLDEAPDIRRDIVLALENLGFHVEASHHEVAPGQHEVDFKFDHALKTADSVVTFKMTIKNIAKKHGLRATFMPKPFYGMNGNGMHCHQSVWFNGEPSFYDPNGPYNGLSETCLSYIAGILEHAKALVAITNPTVNSYKRLVPGYEAPVNIAWANKNRSAIIRVPAARGKATRIEFRAPDPTCNPYLAFACMLAAGLDGIKRKLTPPEPVERNIFRMSEEEKKALGIESVPASLKEALDHLECDEVLQKALGDYIYEKWLEIKRCEWDCYRTAVTDWEVNNYLIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6V167</accession>
    <name>N6V167_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphoesterase</fullName>
            <ecNumber evidence="4">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_04795</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96028.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592012.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V167"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00977"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.938"/>
    <dbReference type="OrthoDB" id="9959at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00841">
        <property type="entry name" value="MPP_YfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053193">
        <property type="entry name" value="MetalloPDE_YfcE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041802">
        <property type="entry name" value="MPP_YfcE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020935">
        <property type="entry name" value="PdiEstase_YfcE_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43165">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43165:SF1">
        <property type="entry name" value="PHOSPHODIESTERASE MJ0936"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01269">
        <property type="entry name" value="UPF0025"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="5">
        <location>
            <begin position="1"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96028.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="165" mass="18933" checksum="DAD340E4EDE9D9DE" modified="2013-06-26" version="1">MKIGIISDTHDNLVNIRKAIEEFKKEGIDLLIHCGDFVSLFVIKEFYNINCDIIAVYGNNDGEREKLKEMLKNINKNNTIADFQSVEIDGLKFFITHGHYQEILDLAIASKLYDVIIYGHTHERVFENIDNTLIINPGECCGYLTGEATIGILDTEKREYKEIFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1J3</accession>
    <name>N6V1J3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme F420 hydrogenase subunit gamma</fullName>
            <ecNumber evidence="3">1.12.98.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_03753</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FrhG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.1" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96143.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591336.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1J3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00383"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.733"/>
    <dbReference type="OrthoDB" id="38261at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="entry name" value="NADH:ubiquinone oxidoreductase-like, 20kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017681">
        <property type="entry name" value="Coenz_F420_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051349">
        <property type="entry name" value="Hydrogenase_assoc-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03294">
        <property type="entry name" value="FrhG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42845">
        <property type="entry name" value="COENZYME F420-REDUCING HYDROGENASE, GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42845:SF2">
        <property type="entry name" value="F420-NON-REDUCING HYDROGENASE VHU SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00614">
        <property type="entry name" value="NIHGNASESMLL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="163"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="4">
        <location>
            <begin position="195"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="NCBIfam" id="TIGR03294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96143.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="225" mass="24625" checksum="9C51FE439068345A" modified="2013-06-26" version="1">MVKIAHVQLCSCCGCLVSLTDLYERLLEVLDKVELVYSQTLMDVREIPECDIAIVEGAVCLDDHHSLEVAKEVRERAKIVIALGSCAVTGGITRFCKGNQKPKPVHNAFTPLSEVINVDLSIPGCPPSPDIIYNVILALLNNDVDYLSLFVDKKTKACGCDLITEVVNKDLCMGCGTCAASCPTRAIEMIDGRPVIKETCIKCGVCSFQCPRIRYPKFLENIEGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1S6</accession>
    <name>N6V1S6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">asparagine synthase (glutamine-hydrolyzing)</fullName>
            <ecNumber evidence="1">6.3.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">J422_03453</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>L-aspartate + L-glutamine + ATP + H2O = L-asparagine + L-glutamate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12228"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58048"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.4" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000021">
        <property type="protein sequence ID" value="ENN96243.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591073.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1S6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00934"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004066">
        <property type="term" value="F:asparagine synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006529">
        <property type="term" value="P:asparagine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01991">
        <property type="entry name" value="Asn_synthase_B_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00712">
        <property type="entry name" value="AsnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006426">
        <property type="entry name" value="Asn_synth_AEB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001962">
        <property type="entry name" value="Asn_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050795">
        <property type="entry name" value="Asn_Synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033738">
        <property type="entry name" value="AsnB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01536">
        <property type="entry name" value="asn_synth_AEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772">
        <property type="entry name" value="ASPARAGINE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11772:SF2">
        <property type="entry name" value="ASPARAGINE SYNTHETASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00733">
        <property type="entry name" value="Asn_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001589">
        <property type="entry name" value="Asn_synthetase_glu-h"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0061">Asparagine biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="2" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="12">
        <location>
            <begin position="2"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="10">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="101"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="270"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001589-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96243.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="436" mass="50003" checksum="2506C32DC30579B1" modified="2013-06-26" version="1" fragment="single">MCGIVGIVAKEKIEGNYIVEMLKAIEHRGKDGYGFYIDGDVYYNNYPNKDINKIALAHCRLAIVGNREQPIIMDNCVLVCNGEIYNYEDLEKRYNLNVGTDVEVILPLYKIGMLKELDGDFAFAIYDNNRLILGRDFIGVKPLFFVNSEKYFAFASERKALWKLLIELDGIEKDIDKLNSKINRVEPNSILIYDINNHKYNSKRIKDFDIKSLNIDYKEAKNILENKIKRAVYKRVKGLKKVGIIFSGGVDSSLITVLASKYTDVVLYTVGIEGSEDLYYAERLAKELNLPLKKKIIREEEYEKYLLKVVKAIDELDLMKIGVGLPIYIASELANKDGIKVILAGQGADELFAGYAKYLKLDKNELEKKLIEDVINISKVNLERDDHCSMANTVELRVPYLDEDVIKFSLSLPIEYKISKERKRILRDIAKNYLPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1Y7</accession>
    <name>N6V1Y7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5'-deoxyadenosine deaminase</fullName>
            <shortName evidence="4">5'-dA deaminase</shortName>
            <ecNumber evidence="4">3.5.4.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">5'-methylthioadenosine deaminase</fullName>
            <shortName evidence="4">MTA deaminase</shortName>
            <ecNumber evidence="4">3.5.4.31</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Adenosine deaminase</fullName>
            <ecNumber evidence="4">3.5.4.4</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">S-adenosylhomocysteine deaminase</fullName>
            <shortName evidence="4">SAH deaminase</shortName>
            <ecNumber evidence="4">3.5.4.28</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dadD</name>
        <name evidence="6" type="ORF">J422_03049</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the deamination of three SAM-derived enzymatic products, namely 5'-deoxyadenosine, S-adenosyl-L-homocysteine, and 5'-methylthioadenosine, to produce the inosine analogs. Can also deaminate adenosine. The preferred substrate for this enzyme is 5'-deoxyadenosine, but all these substrates are efficiently deaminated. Likely functions in a S-adenosyl-L-methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions. May also be involved in the recycling of 5'-deoxyadenosine, whereupon the 5'-deoxyribose moiety of 5'-deoxyinosine is further metabolized to deoxyhexoses used for the biosynthesis of aromatic amino acids in methanogens.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>5'-deoxyadenosine + H2O + H(+) = 5'-deoxyinosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:42892"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:82775"/>
            <dbReference type="EC" id="3.5.4.41"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-adenosyl-L-homocysteine + H2O + H(+) = S-inosyl-L-homocysteine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:20716"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:57985"/>
            <dbReference type="EC" id="3.5.4.28"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>S-methyl-5'-thioadenosine + H2O + H(+) = S-methyl-5'-thioinosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:25025"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:48595"/>
            <dbReference type="EC" id="3.5.4.31"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>adenosine + H2O + H(+) = inosine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24408"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16335"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="4">SAH is a product of SAM methyltransferases and is known to be a feedback inhibitor of these enzymes. As a result of this inhibition, organisms have evolved efficient enzymes to metabolize SAH via different pathways. The pathway found in methanogens differs from the canonical pathway, it uses the deamination of S-adenosyl-L-homocysteine to form S-inosyl-L-homocysteine for the regeneration of SAM from S-adenosyl-L-homocysteine. 5'-deoxyadenosine is a radical SAM enzyme reaction product which strongly inhibits radical SAM enzymes. A pathway for removing this product must be present in methanogens where the MTA/SAH nucleosidase which normally metabolizes this compound is absent.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-dependent hydrolases superfamily. MTA/SAH deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.41" evidence="4"/>
    <dbReference type="EC" id="3.5.4.31" evidence="4"/>
    <dbReference type="EC" id="3.5.4.4" evidence="4"/>
    <dbReference type="EC" id="3.5.4.28" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96303.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590754.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1Y7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00157"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.598"/>
    <dbReference type="OrthoDB" id="372084at2157"/>
    <dbReference type="UniPathway" id="UPA00315"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090613">
        <property type="term" value="F:5'-deoxyadenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090614">
        <property type="term" value="F:5'-methylthioadenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004000">
        <property type="term" value="F:adenosine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050270">
        <property type="term" value="F:S-adenosylhomocysteine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01298">
        <property type="entry name" value="ATZ_TRZ_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.140:FF:000014">
        <property type="entry name" value="5-methylthioadenosine/S-adenosylhomocysteine deaminase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01281">
        <property type="entry name" value="MTA_SAH_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023512">
        <property type="entry name" value="Deaminase_MtaD/DadD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF11">
        <property type="entry name" value="AMIDOHYDROLASE-RELATED DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="5">
        <location>
            <begin position="44"/>
            <end position="391"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96303.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="418" mass="47489" checksum="FAD0FCCB686784A7" modified="2013-06-26" version="1">MLLIKNVYVNGKRQDILIEGNKIKEIGEVKKEEEMEVIDGSDKIAIPGLINTHTHIPMTLFRGVADDLPLMEWLNNYIWPMEAKLNEEIVYYGTLLGCIEMIKTGTTTFNDMYFYLEGIAKAVNESGIRAVLAYGMIDLFDEERREREIKNAKKYIKYIKDLNNSRIIPALGPHSAYTCSKELLEEVSKLAKEQNLLIHIHMNETLDEIKIVKEKTGMEPFKYLDSFGFFDNNNVVAAHCVHLTEEEIEIIKRNNISISHNPISNLKLASGIAPIEKLLKKGVNITLGTDGCGSNNNLNLFEEIKTVAILHKGVNLNPRLVKAEEAFKFATENGAKALNIKAGRIEKGYLADIVLIKLNKPFLYPKENIYSHLVYAFNGTVDDVIIDGKIVMRDNKILTLDEEKVYEKFEKAYYKLIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2Q1</accession>
    <name>N6V2Q1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Class III signal peptide-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01905</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96528.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590139.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2Q1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00229"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.375"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026451">
        <property type="entry name" value="ClassIII_w_PIP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04205">
        <property type="entry name" value="classIII_w_PIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96528.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="67" mass="7478" checksum="760CFEDEEAC3D2A8" modified="2013-06-26" version="1">MKNRAQISLEFALLLATVLAVASIVGFYYIKSIAQGSAHYKETTLKSEAVTQTEAMKRAERVKQLME</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6V334</accession>
    <name>N6V334_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter integral membrane subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_01161</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589898.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V334"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00812"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.227"/>
    <dbReference type="OrthoDB" id="27848at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033214">
        <property type="term" value="P:siderophore-iron import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06550">
        <property type="entry name" value="TM_ABC_iron-siderophores_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472:SF25">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN MJ0876-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="FERRIC ENTEROBACTIN TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="61"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="120"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="151"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="202"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="248"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="320"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="346" mass="38438" checksum="B1E8755DA50CAD2B" modified="2013-06-26" version="1">MAIKKRFILTVFLLLVLLFILFYYSLILGSIKLSHNDIINFILNKTTGNKIKDIILIKLRLVRSLSAIIAGVAISVAGVYMQSYFRNPLADPYLTGIASGAALGVVIYGFTNLFLNYSSIWIQIGMAYLFSLITMLIVIKLAEITKQVSTLLICGIMIGAILSGFIDILVYTADILNVENNKLQGFLLWGMGSFNNLTMKEVKIMTFLTLPILLLSYIFLSKKLDAYLLGEMYAMSVGVDIKRLKRYLILLSSFLIASIISFTGPIAFIGLICPLLARMFLKTSKHLYVIPITALFGAVLLLLSDILVRPHVIIKTSIQLPLLAPLSIIGSPIAVILYLKYRRLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3A9</accession>
    <name>N6V3A9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">O-antigen polymerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00866</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589843.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3A9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00861"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.171"/>
    <dbReference type="OrthoDB" id="70767at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002760">
        <property type="entry name" value="O_anti_polymase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01901">
        <property type="entry name" value="O_anti_polymase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="36"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="115"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="207"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="301"/>
            <end position="318"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="330"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="368" mass="42916" checksum="7B3472DA3FEEF677" modified="2013-06-26" version="1">MFKKAFKIEHPVNIIFIGHLIIFLLAFPYYDKLGFSIFKIIFIILSNILTFSIPFILDIKVKINKTIYYISSILLAFISFYGAFVITKCLFLSILYVIFIFIVVEIFLKYYYKKIFNIFLFVIGVLSFLLLVIKYKAIPLLNYNVRMLINYEPLRIISLGALAYSGIESWKYFLLSLVILSLTGYKVGPVILFTSYLIYKKYPIKKITIFGSILILLILIMNKIILSTSGQCWKLSTIEVLCYRAYLDLYIFKSIIENHITTYGSIILTPGGEEKIGELLFSYKHNFTTTMFGTVYMDFNIFSPIFSIIFGIISKFLYEGDWKLYSIYASLLLIYCEIGINYGFLVVILLLFYINICKVIKYEIYNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3B6</accession>
    <name>N6V3B6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">LysR family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_00511</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589784.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3B6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00054"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.98"/>
    <dbReference type="OrthoDB" id="144613at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000001">
        <property type="entry name" value="LysR family transcriptional regulator"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047788">
        <property type="entry name" value="LysR-like_Sec_metab"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="LysR_HTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005119">
        <property type="entry name" value="LysR_subst-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040786">
        <property type="entry name" value="LysR_Sec_metab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30126">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30126:SF40">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR GLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03466">
        <property type="entry name" value="LysR_substrate"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00039">
        <property type="entry name" value="HTHLYSR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="295" mass="33350" checksum="72B3EE560B5282B6" modified="2013-06-26" version="1">MDPKVSYFQTFIIAAKTKSFSKAAKRLGITQGTVSNHIAALEKFFDAQLFLRTPEGVDLTPEGEILFERAEKILDLINEAKMLMRAIHENPEGVIRIYASTTPGEHVLPSIIKEYKNQYKNVDFEITITDSKNCYKALEEGLADIIAVGYLKNENYDYTIIGKDRLVLIVPPNHPFTKREYVRLEDILKEDFIDREEGSGTRETFIKALNEKGYSIMDLNIVMRLGSSSAVITAVSEGYGISIVSEIPAKKAENAGLVKIVPIVDFDVVRYLYLIKSKRPKNPSAVKSFWEFVNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3F8</accession>
    <name>N6V3F8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADPH-dependent F420 reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589704.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3F8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01156"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.11"/>
    <dbReference type="OrthoDB" id="8635at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070967">
        <property type="term" value="F:coenzyme F420 binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008823">
        <property type="term" value="F:cupric reductase (NADH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052851">
        <property type="term" value="F:ferric-chelate reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015677">
        <property type="term" value="P:copper ion import"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006740">
        <property type="term" value="P:NADPH regeneration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010185">
        <property type="entry name" value="NpdG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028939">
        <property type="entry name" value="P5C_Rdtase_cat_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051267">
        <property type="entry name" value="STEAP_metalloreductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01915">
        <property type="entry name" value="npdG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239">
        <property type="entry name" value="DUDULIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14239:SF0">
        <property type="entry name" value="F420-DEPENDENT NADP REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03807">
        <property type="entry name" value="F420_oxidored"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyrroline-5-carboxylate reductase catalytic N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03807"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="220" mass="23763" checksum="AF9D5CA99E628A50" modified="2013-06-26" version="1">MKIAILGGTGDQGFGLALRLAKDNEIIIGSRKKEKAEAAAEKAREILKSKNILAEIKGLENKDAAKEGEIVIISIPYEHILSTIKELKDELKGKIVVSIAVPLATAIGDNPTRLLYPPDGSVAEMIQKVLKDSKVVSAFQNISAKILSDLDSEIECDILVCGDYEDAKKVVIDLINKMGARAIDCGNLEKSRIIEGITALLIAINKKYKIDRAGIKITGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3G4</accession>
    <name>N6V3G4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA polymerase II small subunit</fullName>
            <shortName evidence="15">Pol II</shortName>
            <ecNumber evidence="15">2.7.7.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Exodeoxyribonuclease small subunit</fullName>
            <ecNumber evidence="15">3.1.11.1</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">polB</name>
        <name evidence="18" type="ORF">J422_00100</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13 15">Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single-stranded DNA in the 3' to 5' direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>Exonucleolytic cleavage in the 3'- to 5'-direction to yield nucleoside 5'-phosphates.</text>
            <dbReference type="EC" id="3.1.11.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3 15">Heterodimer of a large subunit and a small subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the DNA polymerase delta/II small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="15"/>
    <dbReference type="EC" id="3.1.11.1" evidence="15"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589709.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3G4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01148"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.20"/>
    <dbReference type="OrthoDB" id="372039at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042575">
        <property type="term" value="C:DNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008310">
        <property type="term" value="F:single-stranded DNA 3'-5' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006308">
        <property type="term" value="P:DNA catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006271">
        <property type="term" value="P:DNA strand elongation involved in DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07386">
        <property type="entry name" value="MPP_DNA_pol_II_small_archeal_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.21.50:FF:000003">
        <property type="entry name" value="DNA polymerase II small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00325">
        <property type="entry name" value="DNApol_II_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024826">
        <property type="entry name" value="DNA_pol_delta/II_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011149">
        <property type="entry name" value="Pol2_small_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003118">
        <property type="entry name" value="PRK04036.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003119">
        <property type="entry name" value="PRK04036.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10416:SF0">
        <property type="entry name" value="DNA POLYMERASE DELTA SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000803">
        <property type="entry name" value="Arc_Pol2_small"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="6 15" id="KW-0235">DNA replication</keyword>
    <keyword evidence="11 15" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 15" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="9 15" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7 15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5 15 18" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 15 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="17">
        <location>
            <begin position="295"/>
            <end position="500"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="16">
        <location>
            <begin position="78"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00024817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ENN96803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="562" mass="65625" checksum="4E6F6E64E270E886" modified="2013-06-26" version="1">MINKFLEIEVLLTPDVYKALKQLKNEDIEMIIKKIKEFKRYNNKLILLDKNFLEIFTLNSLDEIINKYKDFDFISYYTGEKEEKKEEIKEEVIKEREEKEETKEEKEEVKEELPTIEEKEKTKNESLIEIRESFKSRINWIAKDIETKVKIYEDTDVSGKSTCTGSIEDFIKYFRDRYERLKKFIERKAKRKAKAIKDIRKVEEDVFTVGLVSDVSQRKNGDLLVRIEDLEDEILLILQKDKINNGELPDDILLDEVIGATGMVKNGVMYVNEIIRPSPTPKEPKRIDEEIYMCFLSDIHVGSNEFLKKDFEKFIRFLNGETNNGFEERIVSRLKYICIAGDLVDGVGVYPGQEEDLYEIDIIKQYEEIANFIEQIPEHIAIIISPGNHDAVRPAEPQPRLPDEITKLFNRDNIYFVGNPCVLNIHDFNTLLYHGRSFDDLVSQVRTASYENPTTIMKELIKRRLLCPTYGGRCPIAPEHKDYLVIDKDIDILHTGHIHINGYGIYRGIVMVNSGTFQEQTDFQKRMGIKPTPSIVPIINLSKVGQKGHYIEWDKGIVELRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3I2</accession>
    <name>N6V3I2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Cell division protein SepF</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589724.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3I2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01133"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.35"/>
    <dbReference type="OrthoDB" id="59585at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17700" checksum="968A1F0CB556B002" modified="2013-06-26" version="1">MVFEKIIKKIRGNKDLPKPVEVPEEEEYEVIGEETQTVILDEEEKKEEVKEIIKIEKILPKLFVVRIKHPLDFREIKGQIPDYDVIIINFEEVPEEAIIKEITDFKSYLDTINYRYGLVSENVLLCFRNDVELDKYVSKIKEDVEEIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP40</accession>
    <name>N6VP40_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Imidazoleglycerol-phosphate dehydratase</fullName>
            <shortName evidence="6">IGPD</shortName>
            <ecNumber evidence="6">4.2.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 7" type="primary">hisB</name>
        <name evidence="7" type="ORF">J422_06912</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:11040"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="EC" id="4.2.1.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 6/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the imidazoleglycerol-phosphate dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.19" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000064">
        <property type="protein sequence ID" value="ENN95610.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594593.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP40"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00931"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1340"/>
    <dbReference type="OrthoDB" id="103579at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00011"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004424">
        <property type="term" value="F:imidazoleglycerol-phosphate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07914">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000001">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.40:FF:000003">
        <property type="entry name" value="Imidazoleglycerol-phosphate dehydratase HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.40">
        <property type="entry name" value="Imidazole glycerol phosphate dehydratase, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00076">
        <property type="entry name" value="HisB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038494">
        <property type="entry name" value="IGPD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000807">
        <property type="entry name" value="ImidazoleglycerolP_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020565">
        <property type="entry name" value="ImidazoleglycerP_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002111">
        <property type="entry name" value="PRK00951.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002113">
        <property type="entry name" value="PRK00951.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002114">
        <property type="entry name" value="PRK00951.2-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133:SF2">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23133">
        <property type="entry name" value="IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00475">
        <property type="entry name" value="IGPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00954">
        <property type="entry name" value="IGP_DEHYDRATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00955">
        <property type="entry name" value="IGP_DEHYDRATASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95610.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="192" mass="21774" checksum="16D1A4172D5BF93E" modified="2013-06-26" version="1">MRVFEVARETKETCIYLKINIDGRGKYNIDTGIEFFDHLLSAFAKHGCFDLIVKARGDLEVDDHHTVEDVGICLGLALNQIERKNIKRFGWAIIPMDDARTMMALDLGGRSYCLAKYKPRREFIGGLSLENINHFFSSVANYGFLNIHYEVIGENEHHKLESLFKAFGVALDMATQIDCRKGIVSTKGEVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ45</accession>
    <name>N6VQ45_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">A-type ATP synthase subunit D</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">atpD</name>
        <name evidence="12" type="ORF">J422_04670</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the V-ATPase D subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96005.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591959.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ45"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00952"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.913"/>
    <dbReference type="OrthoDB" id="117390at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.3240:FF:000007">
        <property type="entry name" value="V-type ATP synthase subunit D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00271">
        <property type="entry name" value="ATP_synth_D_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002699">
        <property type="entry name" value="V_ATPase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001545">
        <property type="entry name" value="PRK00373.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00309">
        <property type="entry name" value="V_ATPase_subD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11671">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01813">
        <property type="entry name" value="ATP-synt_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 10" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="11"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00059506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00271"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96005.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="216" mass="25180" checksum="CDC21CB3EFBC0531" modified="2013-06-26" version="1">MQRVNPTRMELLKLKNKIKLAEKGHKLLKQKRDALIMEFFQIIDLAQDLREKVEKKLEEAYKDLTLAEVSMGTLAVKEASFAAKNDKIEIDVDTKNIMGVTVPTFEIYNVKRKVGERGYSPYGVSSKLDEAAKKFEEALELITELAEIETSVRLLAEEIIKTKRRVNALEYIIIPRLKSLKKYISMRLDEMERENFFRLKLIKSRIEKREEEGENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQE2</accession>
    <name>N6VQE2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">A-type ATP synthase subunit B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">atpB</name>
        <name evidence="12" type="ORF">J422_04428</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane. The B chain is a regulatory subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cell membrane</location>
            <topology evidence="8">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96085.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591802.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQE2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00638"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.868"/>
    <dbReference type="OrthoDB" id="32941at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18112">
        <property type="entry name" value="ATP-synt_V_A-type_beta_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18118">
        <property type="entry name" value="ATP-synt_V_A-type_beta_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01135">
        <property type="entry name" value="V_A-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00310">
        <property type="entry name" value="ATP_synth_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005724">
        <property type="entry name" value="ATPase_A1-cplx_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022879">
        <property type="entry name" value="V-ATPase_su_B/beta"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01041">
        <property type="entry name" value="ATP_syn_B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003235">
        <property type="entry name" value="PRK04196.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43389:SF4">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039114">
        <property type="entry name" value="V-ATPsynth_beta/V-ATPase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="3 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 8" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="10">
        <location>
            <begin position="10"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="9">
        <location>
            <begin position="132"/>
            <end position="350"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="11">
        <location>
            <begin position="356"/>
            <end position="454"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96085.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="460" mass="50902" checksum="6DA96371280446C5" modified="2013-06-26" version="1">MSAVEYTSVKSVAGPLLIVEGVEGAAYGEIVEVITPSGEKRMGQVLEAREGLAVVQVFEGTTGLNTKNTKVRFTGRTAKIGVSIEMLGRIFNGRGKPIDGGPEIVPEKELDINGYPLNPVSRKYPSDFIQTGISTIDGMNTLVRGQKLPIFSGSGLPHNQLAVQIARQAKVRGEGEKFAVVFAAMGITSEEANFFMEEFRRTGALEKAVVFINLASDPAIERILTPRIALTVAEYLAFEKDMHVLVILTDMTNYCEALREIAAARNEVPGRRGYPGYMYTDLATLYERAGRAKGKKGTITQIPILTMPDDDITHPIPDLTGYITEGQIVLSRELHRKGIYPPIDVLPSLSRLAGSGQGPGKTREDHKKVVNQAYAAYAEGRSLRDLVAVVGEEALTERDKAYLKFADEFEKRFVNQGKDEDRSIEETLDLLWELLAILPEEELKRVDKELIMKYHPKYRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQJ6</accession>
    <name>N6VQJ6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0305 protein J422_03823</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03823</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0305 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96155.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591358.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQJ6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00396"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.749"/>
    <dbReference type="OrthoDB" id="81482at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00763">
        <property type="entry name" value="UPF0305"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019215">
        <property type="entry name" value="DUF2115"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002176">
        <property type="entry name" value="PRK01022.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09888">
        <property type="entry name" value="DUF2115"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004959">
        <property type="entry name" value="UCP004959"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96155.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="158" mass="18939" checksum="428C156F52EEF3ED" modified="2013-06-26" version="1">MKAKELLKKLKEKAENFSIYDIIAIKCFIEKDAKYLPEKYKEDYINAMIKYYIDTLNEIKSKEICEDYEIDEKKLNELINRIESFKKYNSEEEKKFIELSKIICPYLLFIAKKPFHPEYLIFPGGLKIIKDNNIYYCPVKEKQLNEYSLCEFCVAKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR72</accession>
    <name>N6VR72_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Adenosylmethionine-8-amino-7-oxononanoate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_06777</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000061">
        <property type="protein sequence ID" value="ENN95636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594168.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000061.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR72"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00757"/>
    <dbReference type="OrthoDB" id="6534at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004015">
        <property type="term" value="F:adenosylmethionine-8-amino-7-oxononanoate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42684">
        <property type="entry name" value="ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42684:SF17">
        <property type="entry name" value="ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="295" mass="33750" checksum="43F5CE235A750C02" modified="2013-06-26" version="1" fragment="single">MDKKLLEKWDLEYVWHPYTQMKEYKPFIIERGEGNYLIDINGNKYLDAVSSIWCNTFGHSRREIIEAIKKQAEKICHSTLLGASNIPSILLAKKLVEITPKHLTKVFYSEDGAEAVEIAIKIAYQYYYLRGEDRKKFISVKEGYHGDTFGAMSVGGCELFHGIFKPLLFKGYHAYAPYCYRCKYYNWKDTDERNEKGCDMKCLDEMLNLIEEKKDEVFCVILEAGIRGSAGMIPFPDGYIEEVAKACKDNDIILILDEVATGFGRTGKMFFSDNDKLKKLKKPDIICLGKAITGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR85</accession>
    <name>N6VR85_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein uL29</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl29</name>
        <name evidence="6" type="ORF">J422_02539</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uL29 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96415.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590463.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR85"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00765"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.497"/>
    <dbReference type="OrthoDB" id="11736at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00427">
        <property type="entry name" value="Ribosomal_L29_HIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.310:FF:000001">
        <property type="entry name" value="50S ribosomal protein L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.310">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00374">
        <property type="entry name" value="Ribosomal_uL29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050063">
        <property type="entry name" value="Ribosomal_protein_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001854">
        <property type="entry name" value="Ribosomal_uL29"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018254">
        <property type="entry name" value="Ribosomal_uL29_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036049">
        <property type="entry name" value="Ribosomal_uL29_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00012">
        <property type="entry name" value="L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10916">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L35/50S RIBOSOMAL PROTEIN L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10916:SF0">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL29C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00831">
        <property type="entry name" value="Ribosomal_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46561">
        <property type="entry name" value="Ribosomal protein L29 (L29p)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00579">
        <property type="entry name" value="RIBOSOMAL_L29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009254"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96415.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="67" mass="7725" checksum="CFFB3434C5BC5ABC" modified="2013-06-26" version="1">MAILRASELREMSIDDLKKKLIELKKELLKERAHKAAAGAPSNPGRMREIRRTIARILTIMNEKKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRA5</accession>
    <name>N6VRA5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">3-phosphoshikimate 1-carboxyvinyltransferase</fullName>
            <ecNumber evidence="8">2.5.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">5-enolpyruvylshikimate-3-phosphate synthase</fullName>
            <shortName evidence="8">EPSP synthase</shortName>
            <shortName evidence="8">EPSPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">aroA</name>
        <name evidence="10" type="ORF">J422_06336</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate (S3P) to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>3-phosphoshikimate + phosphoenolpyruvate = 5-O-(1-carboxyvinyl)-3-phosphoshikimate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21256"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="EC" id="2.5.1.19"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:21257"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the EPSP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.19" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593506.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRA5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01306"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1233"/>
    <dbReference type="OrthoDB" id="43788at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00089"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003866">
        <property type="term" value="F:3-phosphoshikimate 1-carboxyvinyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01556">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.65.10.10:FF:000012">
        <property type="entry name" value="Pentafunctional AROM polypeptide"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.10">
        <property type="entry name" value="Enolpyruvate transferase domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00210">
        <property type="entry name" value="EPSP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001986">
        <property type="entry name" value="Enolpyruvate_Tfrase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036968">
        <property type="entry name" value="Enolpyruvate_Tfrase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006264">
        <property type="entry name" value="EPSP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023193">
        <property type="entry name" value="EPSP_synthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01356">
        <property type="entry name" value="aroA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090">
        <property type="entry name" value="AROM/DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21090:SF5">
        <property type="entry name" value="PENTAFUNCTIONAL AROM POLYPEPTIDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00275">
        <property type="entry name" value="EPSP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000505">
        <property type="entry name" value="EPSPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00104">
        <property type="entry name" value="EPSP_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00885">
        <property type="entry name" value="EPSP_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Enolpyruvate transferase" evidence="9">
        <location>
            <begin position="5"/>
            <end position="415"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="8">
        <location>
            <position position="307"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="192"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>3-phosphoshikimate</name>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="338"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="380"/>
        </location>
        <ligand>
            <name>phosphoenolpyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00044633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="425" mass="46798" checksum="179CA9424B287672" modified="2013-06-26" version="1">MLIVKKTEHLEGKVNAPPSKSYTHRAVIGASLAEGVSKIKYPLWGEDCLASVRGCESLGAKVKLDKDVWFIEGGNFKTPDDVINVGNSGTTLRILTSISSLIPKGYAILTGDSSIRKRPMQPLLDALKQININAFSSKLNGTAPIIVESGKINGNVVKIRGDISSQFITSLLMLLPFNKEDSEIILTTPLKSKPYIDVTIDILDKFGIKVDETEKGFLVYGNQRYRPIDYIIEGDYSSSSYIIAAGILINSHIEIYNLFKDSKQGDKAIIEIVKDMGADIKVKKDCVIVEGEYNLRGIDVDIKDIPDLLPTIAVLACFAEGKTEIYNGEHVRYKETDRIKACYTELKKMGANIKEKEDGLIIEGVKKLKGARLNTYNDHRLVMAFTVAGLKAEGESIIEGEEAVKVSFPNFIEVMKSLGANIEVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRF1</accession>
    <name>N6VRF1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">N-(5'-phosphoribosyl)anthranilate isomerase</fullName>
            <shortName evidence="8">PRAI</shortName>
            <ecNumber evidence="8">5.3.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trpF</name>
        <name evidence="10" type="ORF">J422_06274</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:21540"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="EC" id="5.3.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 8">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 8">Belongs to the TrpF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.24" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593359.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRF1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00283"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1222"/>
    <dbReference type="OrthoDB" id="27513at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00042"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00405">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00135">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001240">
        <property type="entry name" value="PRAI_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044643">
        <property type="entry name" value="TrpF_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42894:SF1">
        <property type="entry name" value="N-(5'-PHOSPHORIBOSYL)ANTHRANILATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00697">
        <property type="entry name" value="PRAI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 8" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 8 10" id="KW-0413">Isomerase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="N-(5'phosphoribosyl) anthranilate isomerase (PRAI)" evidence="9">
        <location>
            <begin position="3"/>
            <end position="214"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="220" mass="25157" checksum="2E825D4DBD28B1B9" modified="2013-06-26" version="1">MKIKICGITNKEDLVYIANKVHAVGFIIDVPVKTPRKITLDKAIELKQYLPIFTSLVAVIMPESLEEVLNIYKNLKPHAIQLHGFENLDFVKEIYKLKKEGIIDSSVIKVIHISEEDDFNSILKKTKEFEKYVDAILVDTKIEKIKIIGKTHNWEVSKRLRESIKKPLILAGGINKDNVIEAIKKVKPYAIDVSSSLESEPGKKDFKKVDEFLEVVKKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRJ1</accession>
    <name>N6VRJ1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Undecaprenyl-diphosphatase</fullName>
            <ecNumber evidence="3 12">3.6.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 12">Undecaprenyl pyrophosphate phosphatase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">uppP</name>
        <name evidence="13" type="ORF">J422_01735</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the dephosphorylation of undecaprenyl diphosphate (UPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>di-trans,octa-cis-undecaprenyl diphosphate + H2O = di-trans,octa-cis-undecaprenyl phosphate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28094"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58405"/>
            <dbReference type="ChEBI" id="CHEBI:60392"/>
            <dbReference type="EC" id="3.6.1.27"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the UppP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.27" evidence="3 12"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96500.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590105.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRJ1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00198"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.341"/>
    <dbReference type="OrthoDB" id="65864at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050380">
        <property type="term" value="F:undecaprenyl-diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01006">
        <property type="entry name" value="Undec_diphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003824">
        <property type="entry name" value="UppP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001389">
        <property type="entry name" value="PRK00281.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001390">
        <property type="entry name" value="PRK00281.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00753">
        <property type="entry name" value="undec_PP_bacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622">
        <property type="entry name" value="UNDECAPRENYL-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30622:SF3">
        <property type="entry name" value="UNDECAPRENYL-DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02673">
        <property type="entry name" value="BacA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="13" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="7"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="40"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="71"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="96"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="137"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="175"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="205"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="238"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010621"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00032707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96500.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="257" mass="29180" checksum="8FE0AE5C8BEA22B9" modified="2013-06-26" version="1">MSILEAIVLSFVEGLTEFLPISSTAHLIVVSYFLHLPQNSININFEITIQLAAILAVIFLYNKKLYYDLEIWKKVVISFIPIAILGFLFHKQIYKLFNIYIVALAFIIGGLAFIIIEKFYKVECKINSLEKVDYKRALIIGLFQCLALIPGTSRSGATIIGGMLLKLDRKTATEFSFLSAIPVMLSATIFSLYKNIDKIGNSDITVLIIGFFMSFITAIFSVRWLLNYVRTHNFIPFGIYRIIFGLFLLILYSTALQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRK3</accession>
    <name>N6VRK3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA-guanine(15) transglycosylase</fullName>
            <ecNumber evidence="7">2.4.2.48</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">7-cyano-7-deazaguanine tRNA-ribosyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Archaeal tRNA-guanine transglycosylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tgtA</name>
        <name evidence="9" type="ORF">J422_01795</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Exchanges the guanine residue with 7-cyano-7-deazaguanine (preQ0) at position 15 in the dihydrouridine loop (D-loop) of archaeal tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>guanosine(15) in tRNA + 7-cyano-7-carbaguanine = 7-cyano-7-carbaguanosine(15) in tRNA + guanine</text>
            <dbReference type="Rhea" id="RHEA:43164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10371"/>
            <dbReference type="Rhea" id="RHEA-COMP:10372"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:45075"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:82850"/>
            <dbReference type="EC" id="2.4.2.48"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the archaeosine tRNA-ribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.48" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96510.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590116.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRK3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00209"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.353"/>
    <dbReference type="OrthoDB" id="6871at2157"/>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002099">
        <property type="term" value="P:tRNA wobble guanine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21149">
        <property type="entry name" value="PUA_archaeosine_TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1020.10">
        <property type="entry name" value="ArcTGT, C1 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.90">
        <property type="entry name" value="ArcTGT, C2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01634">
        <property type="entry name" value="TgtA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050076">
        <property type="entry name" value="ArchSynthase1/Queuine_TRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038370">
        <property type="entry name" value="ArcTGT_C1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029402">
        <property type="entry name" value="TGT_C2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038250">
        <property type="entry name" value="TGT_C2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004804">
        <property type="entry name" value="TgtA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002616">
        <property type="entry name" value="tRNA_ribo_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00432">
        <property type="entry name" value="arcsn_tRNA_tgt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00449">
        <property type="entry name" value="tgt_general"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499:SF1">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01702">
        <property type="entry name" value="TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14810">
        <property type="entry name" value="TGT_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88802">
        <property type="entry name" value="Pre-PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="6 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="PUA" evidence="8">
        <location>
            <begin position="573"/>
            <end position="647"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="124"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="195"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96510.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="648" mass="75954" checksum="C2DFEE0968E77B74" modified="2013-06-26" version="1">MRFEIKYRDGMGRVCLLEINNKKIETPTIMPVVHPNPKKQSVPIDFIKKLADIVITNSYIIYKNKFLREIAEKLGVHKLLNFDKIVVTDSGSYQLSVYGKIDVEPNEIIEFQERIGVDVGTILDIPTPPDVDKEKAERDLEETLRRAKEAIKLREERGYRLLLNGTIQGSTYLDLRKKSAIEMSKLNFDIYPIGAVVPLMESYRFREVVEIIINSKMYLPNNKPIHLFGCGHPMFFSLAVLLGCDLFDSAAYSLYAKDGRYLTETGTLHLEEMKDLKEFPCSCPVCLEYKPNELYNLDEKERERLLAEHNLYVTFEEINKIKEAIKEGSLWEFVEKRVRAHPKLLEAYRILKNYMEYIEKFDPVTKKSAFFYTGIESLYRPEVLRHKKRLKRIKYNKVYITNISSKVERPYSEHINIVKTDVDILIKHPIFGLIPYYIDTIYPLSQHEAPELYDFEKNINDRFIEEFINWLRKKIGEENILDIRSYNYYTSLFSEDFDSNAYRIRKMLQYQYGYDILEGVNVRVIRSKNTGRLRKVLNENGDLLFSIRSHDNFLIPSKLGAKLLWEKIPFPKYRVVVNKEAEPFVKKGRNVFAKFIVDADEELRPYEEVIVVNENDELLAYGTNILNGVEMREFNNGLAVKVRGGIYE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRL7</accession>
    <name>N6VRL7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Small ribosomal subunit protein eS28</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rps28e</name>
        <name evidence="6" type="ORF">J422_01865</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eS28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96520.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590127.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRL7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00221"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.367"/>
    <dbReference type="OrthoDB" id="7620at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04457">
        <property type="entry name" value="S1_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000145">
        <property type="entry name" value="30S ribosomal protein S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00292">
        <property type="entry name" value="Ribosomal_eS28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000289">
        <property type="entry name" value="Ribosomal_eS28"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028626">
        <property type="entry name" value="Ribosomal_eS28_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003080">
        <property type="entry name" value="PRK04007.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10769:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES28"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01200">
        <property type="entry name" value="Ribosomal_S28e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00961">
        <property type="entry name" value="RIBOSOMAL_S28E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96520.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="75" mass="8440" checksum="A0C7A3AFE2CBA410" modified="2013-06-26" version="1">MEDEFVYKEAVAAEVIEVIGRTGVYGEVIQVRCKILGGKDAGRVLIRNVKGPVKVGDIIMLRETEREARPLDKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRP8</accession>
    <name>N6VRP8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Auxin efflux carrier</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_02010</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the auxin efflux carrier (TC 2.A.69) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96545.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590177.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRP8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00247"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.394"/>
    <dbReference type="OrthoDB" id="147743at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004776">
        <property type="entry name" value="Mem_transp_PIN-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36838">
        <property type="entry name" value="AUXIN EFFLUX CARRIER FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36838:SF3">
        <property type="entry name" value="TRANSPORTER AUXIN EFFLUX CARRIER EC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03547">
        <property type="entry name" value="Mem_trans"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="30"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="61"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="156"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="278"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96545.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="301" mass="33403" checksum="75267085A9C744D5" modified="2013-06-26" version="1">METVLIIISLVLIGYILKFFNILKESDRRILNNIVIYIAMPSTIFLSILKNLSKSEVIIFLKLPIALFFSFLMCGIISYFLGKFLNLDNKKLGSLILISMLGNTGFLGYPVVLNLFGDEGLVRAIFCDLGSVFITMLVGSYVGVKFGKGGRNVIREVLTFPPLITAIFSIILVLFNFKLTYLPNFLLKTINYLSSTTVPLIMLSLGISLSPNAMRFGIFFGILASIFRFLIAPAFSLSISELLNIKGLERDVLLVESSMPSAMMSLVLGSLYDLDVKIISSAIFITTVLSLFIIALWELIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRQ5</accession>
    <name>N6VRQ5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL23</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpl23</name>
        <name evidence="9" type="ORF">J422_05593</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds to 23S rRNA. One of the proteins that surrounds the polypeptide exit tunnel on the outside of the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Part of the 50S ribosomal subunit. Contacts protein L29.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uL23 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592680.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRQ5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00275"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1091"/>
    <dbReference type="OrthoDB" id="7751at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.330:FF:001084">
        <property type="entry name" value="50S ribosomal protein L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_A">
        <property type="entry name" value="Ribosomal_uL23_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01369_B">
        <property type="entry name" value="Ribosomal_uL23_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012677">
        <property type="entry name" value="Nucleotide-bd_a/b_plait_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019985">
        <property type="entry name" value="Ribosomal_uL23"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013025">
        <property type="entry name" value="Ribosomal_uL23-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012678">
        <property type="entry name" value="Ribosomal_uL23/eL15/eS24_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001014">
        <property type="entry name" value="Ribosomal_uL23_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011118">
        <property type="entry name" value="PRK14548.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03636">
        <property type="entry name" value="uL23_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11620">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L23A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00276">
        <property type="entry name" value="Ribosomal_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54189">
        <property type="entry name" value="Ribosomal proteins S24e, L23 and L15e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00050">
        <property type="entry name" value="RIBOSOMAL_L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003934"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="86" mass="9962" checksum="D75A42F090FB962F" modified="2013-06-26" version="1">MDPFDVIKYPIVSEKTLRLIENENKLVFIVDRRATKQDIKRAMKELFDVEVEKVNTLITPKGEKKAYVKLKKDYDASKIAASLGIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRS2</accession>
    <name>N6VRS2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0146 protein J422_05549</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_05549</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0146 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000042">
        <property type="protein sequence ID" value="ENN95861.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592613.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRS2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00751"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1083"/>
    <dbReference type="OrthoDB" id="59816at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00341">
        <property type="entry name" value="UPF0146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005353">
        <property type="entry name" value="UPF0146"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003165">
        <property type="entry name" value="PRK04148.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03686">
        <property type="entry name" value="UPF0146"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016725">
        <property type="entry name" value="UCP016725"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006969"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95861.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="132" mass="15418" checksum="49F30D610444ECDA" modified="2013-06-26" version="1">MKNEVVAEFIRNIAKEYKFKNIVEVGVGYNYSVALSLREFNIFVVDINENAIKKARMMGLKGYVDDVFNPNIYIYKNVDLIYSIRPPIELQLAILELAKIVNCKVIIRPMYNEPPLELKLKNYKGEIFYLSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS39</accession>
    <name>N6VS39_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Polymerase/histidinol phosphatase N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00951</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589860.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS39"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00584"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.187"/>
    <dbReference type="OrthoDB" id="9968at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042578">
        <property type="term" value="F:phosphoric ester hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07432">
        <property type="entry name" value="PHP_HisPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004013">
        <property type="entry name" value="PHP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050243">
        <property type="entry name" value="PHP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004981">
        <property type="entry name" value="PRK06361.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36928">
        <property type="entry name" value="PHOSPHATASE YCDX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36928:SF1">
        <property type="entry name" value="PHOSPHATASE YCDX-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02811">
        <property type="entry name" value="PHP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Polymerase/histidinol phosphatase N-terminal" evidence="1">
        <location>
            <begin position="3"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="220" mass="24954" checksum="3A9DE23E8C8AE3EC" modified="2013-06-26" version="1">MRYDFHTHTVFSDGELIPSELVRRAIKLKHKAIAITDHADFSNYKELIDKALMAKEELKRYWEIEIIAGIEITHVPPKAIPKLAKKAKDYGAEIVLVHGETIVEPVEEKTNYYASISEIDILAHPGFIDKDTAENLKENNIFLEITSRKGHCLTNGVVLNIAREYNIPFLINSDTHAPNDLISPDFAEKVAYGCGMTKKEVEKALLEYPKELLNRLTNIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS59</accession>
    <name>N6VS59_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Molybdopterin dinucleotide-binding region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00696</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589815.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS59"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00896"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.134"/>
    <dbReference type="OrthoDB" id="116806at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043546">
        <property type="term" value="F:molybdopterin cofactor binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053637">
        <property type="entry name" value="Archaeal_lipid_biosynth_FwdD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012040">
        <property type="entry name" value="Formylmethanofuran_DH_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006657">
        <property type="entry name" value="MoPterin_dinucl-bd_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF042908">
        <property type="entry name" value="FMD_DH_FwdD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01568">
        <property type="entry name" value="Molydop_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015873">
        <property type="entry name" value="FwdD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin dinucleotide-binding" evidence="1">
        <location>
            <begin position="3"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="130" mass="15093" checksum="C67F9DADFDDCA848" modified="2013-06-26" version="1">MKFKLNTGRTIWQGQGIEAGKNLELYRKAAAIAYMNEEDMKKLNVKEGDKIKVKSEFGEVVVYVKKANERLPEGSIYIPMGPWANLVVHPYTDSTGMPYFKGDYDYYVEVEKTDEEVLSMEELMKKYYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSC4</accession>
    <name>N6VSC4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribose-phosphate pyrophosphokinase</fullName>
            <shortName evidence="11">RPPK</shortName>
            <ecNumber evidence="11">2.7.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">5-phospho-D-ribosyl alpha-1-diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphoribosyl diphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Phosphoribosyl pyrophosphate synthase</fullName>
            <shortName evidence="11">P-Rib-PP synthase</shortName>
            <shortName evidence="11">PRPP synthase</shortName>
            <shortName evidence="11">PRPPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">prs</name>
        <name evidence="15" type="ORF">J422_04248</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the biosynthesis of the central metabolite phospho-alpha-D-ribosyl-1-pyrophosphate (PRPP) via the transfer of pyrophosphoryl group from ATP to 1-hydroxyl of ribose-5-phosphate (Rib-5-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>D-ribose 5-phosphate + ATP = 5-phospho-alpha-D-ribose 1-diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15609"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 Mg(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the ribose-phosphate pyrophosphokinase family. Class III (archaeal) subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.1" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96051.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591730.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSC4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00673"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.831"/>
    <dbReference type="OrthoDB" id="371997at2157"/>
    <dbReference type="UniPathway" id="UPA00087">
        <property type="reaction ID" value="UER00172"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002189">
        <property type="term" value="C:ribose phosphate diphosphokinase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004749">
        <property type="term" value="F:ribose phosphate diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006015">
        <property type="term" value="P:5-phosphoribose 1-diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006164">
        <property type="term" value="P:purine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000074">
        <property type="entry name" value="Ribose-phosphate pyrophosphokinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00583_A">
        <property type="entry name" value="RibP_PPkinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029099">
        <property type="entry name" value="Pribosyltran_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005946">
        <property type="entry name" value="Rib-P_diPkinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037514">
        <property type="entry name" value="Rib-P_diPkinase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002095">
        <property type="entry name" value="PRK00934.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01251">
        <property type="entry name" value="ribP_PPkin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210">
        <property type="entry name" value="RIBOSE-PHOSPHATE DIPHOSPHOKINASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10210:SF32">
        <property type="entry name" value="RIBOSE-PHOSPHATE PYROPHOSPHOKINASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13793">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01400">
        <property type="entry name" value="Pribosyltran_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11 15" id="KW-0418">Kinase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11 12" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 11 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Ribose-phosphate pyrophosphokinase N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="13">
        <location>
            <begin position="143"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="33"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="90"/>
            <end position="91"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="214"/>
            <end position="218"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU004324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF13793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN96051.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="282" mass="31224" checksum="7DC843A4FDDB1086" modified="2013-06-26" version="1">MIVAGSQSQELAYKVSKILNRKYARVEYKRFPDSEIYVRLLDSVEDEVVLINSQINQNDAILETILLADALRDEGAKEITLVAPYLAYARQDKKFNPGEAVSIRILAKIYSKLFDKVITINPHEKHIEEFFDVPFVYGDAIPKLAEYVKDKLNNPIVLAPDQGALDFAKTAADILNAEYDYLEKTRISPTEIKIAPKTLDVNGRDVLIIDDIISTGGTMANAVKLLKDQGAKKIIAACVHPLLIGDALNKLYSAGVEEVIGTDTYKSEVSKVSVADIIAKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSD6</accession>
    <name>N6VSD6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">THIF-type NAD/FAD binding fold domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589696.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSD6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01165"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1"/>
    <dbReference type="OrthoDB" id="63188at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008641">
        <property type="term" value="F:ubiquitin-like modifier activating enzyme activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01483">
        <property type="entry name" value="E1_enzyme_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000594">
        <property type="entry name" value="ThiF_NAD_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035985">
        <property type="entry name" value="Ubiquitin-activating_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012028">
        <property type="entry name" value="UCP006529_dinclt"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00899">
        <property type="entry name" value="ThiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006529">
        <property type="entry name" value="UCP006529_dinclt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69572">
        <property type="entry name" value="Activating enzymes of the ubiquitin-like proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="THIF-type NAD/FAD binding fold" evidence="1">
        <location>
            <begin position="13"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="201" mass="22673" checksum="308052B5B79C9E74" modified="2013-06-26" version="1">MDVEELEKKLVPRGEVSLIGCGRLGIRVAFDLLEVHRGGVKKLYVFDGAKIEENDIIHRKLGGLVGEYKVEFLKRFFGNRVEAYNVNIGKDNLHLIKGDVTIICIAGGDTIPITREIIKYCWKKGIKTIGTNGVFGLEENIKVEDAYYCKGPAELLKLEREGHIVVGTGKFIKDFEPITPYTLDEIAKKIVIECLRILRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSI5</accession>
    <name>N6VSI5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04143</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000027">
        <property type="protein sequence ID" value="ENN96121.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSI5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01346"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.811"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96121.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="55" mass="6818" checksum="FDCAE8D8E811F688" modified="2013-06-26" version="1">MKKYYGKDPIKKIFYNKKNREKIFKIFFIVNIWVWFSIFLGALIFIFLVVKRVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSL5</accession>
    <name>N6VSL5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Hydrogenase assembly chaperone hypC/hupF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03888</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HupF/HypC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96166.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591379.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSL5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00408"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.762"/>
    <dbReference type="OrthoDB" id="43695at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902670">
        <property type="term" value="F:carbon dioxide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.140:FF:000022">
        <property type="entry name" value="Hydrogenase assembly chaperone HybG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019812">
        <property type="entry name" value="Hydgase_assmbl_chp_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001109">
        <property type="entry name" value="Hydrogenase_HupF/HypC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00074">
        <property type="entry name" value="hypC_hupF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35177">
        <property type="entry name" value="HYDROGENASE MATURATION FACTOR HYBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35177:SF2">
        <property type="entry name" value="HYDROGENASE MATURATION FACTOR HYBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01455">
        <property type="entry name" value="HupF_HypC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00445">
        <property type="entry name" value="HUPFHYPC"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159127">
        <property type="entry name" value="HupF/HypC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01097">
        <property type="entry name" value="HUPF_HYPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006018"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96166.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="79" mass="9003" checksum="5664265E74CE5363" modified="2013-06-26" version="1">MCLAIPCKVIDIIEEDGEKYAIAEYKGVKQKAKLTLLEDVKIGDYILIHTGYAIEKLSEEEAKLSLEAWEELFKALEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSZ5</accession>
    <name>N6VSZ5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 14 15">Shikimate kinase</fullName>
            <shortName evidence="14">SK</shortName>
            <ecNumber evidence="4 14 15">2.7.1.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">aroK</name>
        <name evidence="18" type="ORF">J422_03139</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>shikimate + ATP = 3-phosphoshikimate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13121"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:145989"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.71"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 14">Belongs to the GHMP kinase family. Archaeal shikimate kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.71" evidence="4 14 15"/>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96321.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590784.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSZ5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00174"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.616"/>
    <dbReference type="OrthoDB" id="9602at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00088"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004765">
        <property type="term" value="F:shikimate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00370">
        <property type="entry name" value="Shik_kinase_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010189">
        <property type="entry name" value="SK_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01920">
        <property type="entry name" value="Shik_kin_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF3">
        <property type="entry name" value="SHIKIMATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005758">
        <property type="entry name" value="Shikimt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="12 14" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10 14 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="16">
        <location>
            <begin position="57"/>
            <end position="146"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="17">
        <location>
            <begin position="223"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="85"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="NCBIfam" id="TIGR01920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ENN96321.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="279" mass="30218" checksum="3C13EC271A9E48EF" modified="2013-06-26" version="1">MEGSAYALASGTIINAISIGKGSAFGVDLKVYAKVRLIDDGKNIVKGKVLDANIKPNLIERCVKNVLEYFGLDYSAYVETKTEIPIKSGLSSSSAVSNATVLATFDALGEKIDDELVLNLAIKSCFDENLTVTGAYDDATASYYGGITVTDNLKRLIIKRDKLKEDLDIIILLPNYKKNVDVDRMKLIKDYVKIAFNYALKGDYFKALFLNGILYASALNFPTNIIIDALSAGAITAGLSGTGPSYVAIAENGEKVKEVWKKYGKVIITKPNNEGAKIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT46</accession>
    <name>N6VT46_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_02769</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96366.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT46"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00105"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.543"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050366">
        <property type="entry name" value="BP-dependent_transpt_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386:SF1">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DDPC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43386">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN APPC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="69"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="107"/>
            <end position="130"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="136"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="194"/>
            <end position="219"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="239"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="67"/>
            <end position="262"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96366.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="276" mass="29994" checksum="EC6BF1E968DB49E9" modified="2013-06-26" version="1">MRDDMKTLKLGLFIIGTVALLSLLAPYITENPYKTDMLNRLSPPSLEHPFGTDQLGRDLFAQVLYGARVSLFVAFAITLLSLAIGCLIGAISGYVGGVLDDIIGRIIDTFMSIPDIVLNIAMVGIFLVTLDITSSIWIVILAITITNWVSYARIARGLCLSLKEKEFVILSKACGASDVWILFKHIIPNMINPLIVLATLNIGNVILTISTLGFLGLGIQPPTPELGTILNSGKNYLTVAPWITGFSGLMIMIIVLGFNLIGEGLRDLLEPKSRYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTC6</accession>
    <name>N6VTC6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diphthine synthase</fullName>
            <ecNumber evidence="6">2.1.1.98</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Diphthamide biosynthesis methyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dphB</name>
        <name evidence="9" type="ORF">J422_02115</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 3 S-adenosyl-L-methionine = diphthine-[translation elongation factor 2] + 3 S-adenosyl-L-homocysteine + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:36415"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="Rhea" id="RHEA-COMP:10172"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="ChEBI" id="CHEBI:82696"/>
            <dbReference type="EC" id="2.1.1.98"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the diphthine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.98" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96446.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590223.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTC6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01172"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.416"/>
    <dbReference type="OrthoDB" id="39139at2157"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004164">
        <property type="term" value="F:diphthine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11647">
        <property type="entry name" value="DHP5_DphB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01084">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004551">
        <property type="entry name" value="Dphthn_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00522">
        <property type="entry name" value="dph5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882:SF0">
        <property type="entry name" value="DIPHTHINE METHYL ESTER SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10882">
        <property type="entry name" value="DIPHTHINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036432">
        <property type="entry name" value="Diphthine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 6 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="8">
        <location>
            <begin position="1"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <begin position="112"/>
            <end position="113"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 7">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR036432-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96446.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="256" mass="29009" checksum="89EDBC9CEE0BB944" modified="2013-06-26" version="1">MLILAGLGLYDEQDMTLKTLNFAKKADKIYAEFYTSILTGTSIEKIEEVIGKKIEILYREDIENSYKVVEEAKEKDVMLLTAGDPMVATTHIDLVIEAKKRGIKVIIINAPSIYSAVSITGLQIYKFGKTASIVFPEKNYFPETPYNVIKENLERGLHTLCLLDIRVEKNEKKFMTANEALKVLLEIEKKRKEGVINLDTKAIVLARVGSLEPLIVYGKIRDLIDYNFGKPLHSLIIPGKLHFMEEEALKYLAINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTK5</accession>
    <name>N6VTK5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">RNA-free ribonuclease P</fullName>
            <shortName evidence="5">RNA-free RNase P</shortName>
            <ecNumber evidence="5">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Protein-only RNase P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_01765</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">RNA-free RNase P that catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the HARP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96506.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590111.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTK5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00204"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.347"/>
    <dbReference type="OrthoDB" id="95197at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18691">
        <property type="entry name" value="PIN_VapC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01078">
        <property type="entry name" value="RNA_free_RNase_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014856">
        <property type="entry name" value="RNA_free_RNase_P"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003340">
        <property type="entry name" value="PRK04358.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003343">
        <property type="entry name" value="PRK04358.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03875">
        <property type="entry name" value="RNA_lig_partner"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41173:SF1">
        <property type="entry name" value="RNA-FREE RIBONUCLEASE P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41173">
        <property type="entry name" value="UPF0278 PROTEIN TK1425"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08745">
        <property type="entry name" value="PIN_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="4 5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96506.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="222" mass="25722" checksum="8573A2900381EFBC" modified="2013-06-26" version="1">MQKQRFCIDTSAFTEPSVRKALGFKTITELTDRLLDLIAEARIKLNISCHIPYPSVYKELLGFLESENCPRNVIVKVDTWLIKKTPNRYEIKIPSEIFYEYIKDLRERINKGMRIGEEHIVKSSEAVYDLTKRHPNLSKREIINKVLSKTINTFRNKYRSALRVGTLDSAPDLDVLLLAKELDAAVVASDGGIEKWAQRLGLRFVNAADFPFMLEEYLRHYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTV3</accession>
    <name>N6VTV3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Lactaldehyde dehydrogenase</fullName>
            <ecNumber evidence="4">1.2.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_01475</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>(S)-lactaldehyde + NAD(+) + H2O = (S)-lactate + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:14277"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16651"/>
            <dbReference type="ChEBI" id="CHEBI:18041"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="EC" id="1.2.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the aldehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.22" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000010">
        <property type="protein sequence ID" value="ENN96591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589956.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTV3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01029"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.290"/>
    <dbReference type="OrthoDB" id="6342at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008911">
        <property type="term" value="F:lactaldehyde dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.605.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.309.10">
        <property type="entry name" value="Aldehyde Dehydrogenase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016161">
        <property type="entry name" value="Ald_DH/histidinol_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016163">
        <property type="entry name" value="Ald_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016162">
        <property type="entry name" value="Ald_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015590">
        <property type="entry name" value="Aldehyde_DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051020">
        <property type="entry name" value="ALDH-related_metabolic_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053404">
        <property type="entry name" value="Lactaldehyde_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040648">
        <property type="entry name" value="lactal_redase_Meth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42991">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42991:SF1">
        <property type="entry name" value="ALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00171">
        <property type="entry name" value="Aldedh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53720">
        <property type="entry name" value="ALDH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0520">NAD</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde dehydrogenase" evidence="9">
        <location>
            <begin position="7"/>
            <end position="456"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="460" mass="51097" checksum="B891BF0C878B4057" modified="2013-06-26" version="1">MLINGNWIEREDLEVINPYTLEVIRKIPALSRDEVREAIDIACSCKEDMKKLSITKRFNILMYIAKKIKEKKEELAKLLAIDAGKPIKQARVEVNRSIETFRSAAFYVKEFRNEVIPSDRLIFTRREPIGLVGAITPFNFPLNLSAHKIAPAIAVGNSIVHHPSPKAPLVCIELAKIIYEAFKKYNAPLGGYNLLTGLGEVVGEEIVVNDKVDMISFTGSIKVGEMILEKAKKKVCLELGGVNPNIVLADADIERAVDSLVKGSFIYSGQVCISVGKIIVDESIADKFIKLFLEKTKKLNVGDPLNEKTDLGPVISLEHAKWVEELINKAIEEGAELLLGGKRVKAVVYPTVLKVDNDNIICNTETFAPIAPIVVANEEHMLDYANSEYGLHAAVFTRDINKALYFAENLDYGGVIINDSSLYRQDNMPFGGIKKSGLGREGVRYAMEEMSYIKTVVINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTW7</accession>
    <name>N6VTW7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methanogenesis marker protein 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01383</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589940.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTW7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00012"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.271"/>
    <dbReference type="OrthoDB" id="7433at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.230">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017667">
        <property type="entry name" value="Methan_mark_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003776">
        <property type="entry name" value="YcaO-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03266">
        <property type="entry name" value="methan_mark_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00702">
        <property type="entry name" value="YcaO-type kinase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37809">
        <property type="entry name" value="RIBOSOMAL PROTEIN S12 METHYLTHIOTRANSFERASE ACCESSORY FACTOR YCAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37809:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN S12 METHYLTHIOTRANSFERASE ACCESSORY FACTOR YCAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02624">
        <property type="entry name" value="YcaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51664">
        <property type="entry name" value="YCAO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YcaO" evidence="1">
        <location>
            <begin position="63"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="377" mass="43766" checksum="2B9FF8319A2F4D33" modified="2013-06-26" version="1">MKKIYRSVTYRILPPEETFNNIKPILDKINIKEMKSIMGIDKLEIPVYYVRRVNNNIEYHHYGKGAVDIQAKVSACMEAIERASAKYDENKIKNEPENKIDINSLILPPYSDTDVKDWVEGYDIINSEPIDVPADAVFYPSKGKLFRWHTNGLASGNCLEEAILHGALEVIERDSWSLADLSKKIPTKINPKTDNEIINRLLEKFEKANVKVILKLLPNELNIPVISAIADDDPLLMCIGVGCHLDPEIAVIRALTELAQSRASQLHMKRDDAKRRAKIVKRLSYERAKKINRKWFEYEEEVELEDIENRAKYNLKKDINLLKEELFDHGFDRFIYVDLNKYGVDTVRVIIPKMEVYTIDRDRLSNMAIERMKKLYQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTZ2</accession>
    <name>N6VTZ2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Putative nickel-responsive regulator</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_01091</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Transcriptional regulator.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
        <text evidence="8">Binds 1 nickel ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589886.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTZ2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00824"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.213"/>
    <dbReference type="OrthoDB" id="25654at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010045">
        <property type="term" value="P:response to nickel cation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00476">
        <property type="entry name" value="NikR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050192">
        <property type="entry name" value="CopG/NikR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022988">
        <property type="entry name" value="Ni_resp_reg_NikR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001884">
        <property type="entry name" value="PRK00630.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002169">
        <property type="entry name" value="PRK01002.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002815">
        <property type="entry name" value="PRK02967.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003381">
        <property type="entry name" value="PRK04460.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719:SF2">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0533">Nickel</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="9">
        <location>
            <begin position="5"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor NikR nickel binding C-terminal" evidence="10">
        <location>
            <begin position="57"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF08753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="141" mass="16149" checksum="E94B39994A096E20" modified="2013-06-26" version="1">MADMERISVSLPSKLLKEFDEIIAERGYASRSEAIRDAIRDYIIKHKWIQSLEGERAGAINIIYNHHENDVMEKLTEIQHDYNDIIVATLHIHLDKDHCLETVLVRGDAKKIKELTNKISALKGVKQVKLSIMVPEGHIPE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU25</accession>
    <name>N6VU25_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">GerC2 protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00906</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96686.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589851.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU25"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00593"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.178"/>
    <dbReference type="OrthoDB" id="147504at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF110">
        <property type="entry name" value="RHODANESE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="42"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96686.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="226" mass="26406" checksum="91871A8B0E8594FC" modified="2013-06-26" version="1">MAIEDYYNILAKHYDSIYKNKYMRVVEKKIIESEIDKEDFVLDIGCGTGEQLKIISNAVGLDISIEMAKIAYNKTKKFIVVANAEFLPFKNNTFDAVISFFGALNHSNIYRTVKEVRRVLKKGGLFIFTVANLYDIQWIFKNICNIRKIKKAMKKRRGEIVKIINGKKIKVKTRFYTLNELEKILKENNFEIKYSFGIYDLPFDVIFYKTPIKVFAKYIGFVAKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VWV4</accession>
    <name>N6VWV4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_07147</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000076">
        <property type="protein sequence ID" value="ENN95567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004595082.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000076.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VWV4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01002"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1381"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008713">
        <property type="term" value="F:ADP-heptose-lipopolysaccharide heptosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002201">
        <property type="entry name" value="Glyco_trans_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051199">
        <property type="entry name" value="LPS_LOS_Heptosyltrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30160:SF7">
        <property type="entry name" value="ADP-HEPTOSE--LPS HEPTOSYLTRANSFERASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30160">
        <property type="entry name" value="TETRAACYLDISACCHARIDE 4'-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01075">
        <property type="entry name" value="Glyco_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="169" mass="20205" checksum="6E776BB95C1EC149" modified="2013-06-26" version="1" fragment="single">KDINKWLRLIFKIRKRDYDIVIVLDKHWIFNLTAFLSGIKKRVGFDRFGEGRFLTHKVPYFGRKHEIFYYLDLLNGLEIEPNYDDWKMDIFLSEKEMEFAEKFWRVNNLNNKTVIGVCPGGANNPGIGNDDLRRWDIIKYIELIKKLKENEYEVLLIGGKLIEALKKKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX20</accession>
    <name>N6VX20_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Carbamoyl phosphate synthase large chain, N-terminal section</fullName>
            <ecNumber evidence="10">6.3.4.16</ecNumber>
            <ecNumber evidence="4">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Carbamoyl phosphate synthetase ammonia chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">J422_06713</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Large subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The large subunit (synthetase) binds the substrates ammonia (free or transferred from glutamine from the small subunit), hydrogencarbonate and ATP and carries out an ATP-coupled ligase reaction, activating hydrogencarbonate by forming carboxy phosphate which reacts with ammonia to form carbamoyl phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>hydrogencarbonate + NH4(+) + 2 ATP = carbamoyl phosphate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18029"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CarB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.16" evidence="10"/>
    <dbReference type="EC" id="6.3.5.5" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000057">
        <property type="protein sequence ID" value="ENN95657.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593916.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX20"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00709"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1307"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004087">
        <property type="term" value="F:carbamoyl-phosphate synthase (ammonia) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000001">
        <property type="entry name" value="Carbamoyl-phosphate synthase large chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1030.10">
        <property type="entry name" value="Carbamoyl-phosphate synthetase, large subunit oligomerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036897">
        <property type="entry name" value="CarbamoylP_synth_lsu_oligo_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005483">
        <property type="entry name" value="CPSase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005480">
        <property type="entry name" value="CPSase_lsu_oligo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058047">
        <property type="entry name" value="CPSase_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003671">
        <property type="entry name" value="PRK05294.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405:SF53">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE [AMMONIA], MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11405">
        <property type="entry name" value="CARBAMOYLTRANSFERASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25596">
        <property type="entry name" value="CPSase_L_D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02787">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00098">
        <property type="entry name" value="CPSASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01096">
        <property type="entry name" value="CPSase_L_D3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48108">
        <property type="entry name" value="Carbamoyl phosphate synthetase, large subunit connection domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0436">Ligase</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="ATP-grasp" evidence="17">
        <location>
            <begin position="126"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00057223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00062056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00083945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ENN95657.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="476" mass="52829" checksum="3235BCA1E77D90D9" modified="2013-06-26" version="1">MKVMVFGSGPIVIGQAAEFDYSGSQACKALREEGVYTILINSNPATIQTDIDMADKVYLEPLHPDIVEKIIEKERPDAILPTMGGQTGLNLALELHRRGVLDKYGIKLIGSDIKTIEMAEDRELFAKAMEEINEPIPKHKAVSSVDEALEFAEKIGYPIIVRPAFTLGGTGGGIANNREELIEIVEKGLKYSPIHQVLLDESVIGWKEIELEVMRDKNDTCIIVCGMENLDPMGIHTGESIVVAPVQTLPDETYQTLRDAAIKIIRKLKVEGGCNIQFAVSKDNSEYKVIEVNPRVSRSSALASKATGYPIAKIAAKIAIGKTLDEILNDITKETPASFEPTIDYVVVKIPRWPFDKFKDVDKKLGTSMKSTGEVMAIGRSFEEALQKAIRSLDIGRFGLIGDGKEKNYTNEEIEEILKNPTDERIFVIAEALDRGWSVEKIAELTNIDPFFIKKIKNIVDMKKFLESIKVKDYGN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX43</accession>
    <name>N6VX43_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Group 1 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_06528</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000053">
        <property type="protein sequence ID" value="ENN95682.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX43"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00999"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1270"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46401:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE WBBK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="3">
        <location>
            <begin position="47"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="2">
        <location>
            <begin position="193"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95682.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="365" mass="42454" checksum="B5443649F1560B6B" modified="2013-06-26" version="1">MEENDNLKIIRFKSFRSFLFKKKEKTSHKIYKKTKPFFYLFLNQYINYSSYEIRKNVEKILKKDNFDIIHANNLESILALNCIKTSAKKIAHLRDFGLFCYNRGLENNGNLCSGCSTNNLSSCMYTKGIINKLLLLEMKKRIKILSKLSSFDLLIAISKFVKQQYVNILGVDENKIEVIYNPISYDIVSNLSKEETRKLLNLPKNKKIILFVGSLIEKKGAHLIPKIAEKLEDYLFVVIGDGILKELFLKNSKDNIIYCGYLPISKIKHFYRASDILLVPSLWHEPFGRVVMEGAYNGCYVIGSNRGGIPEVIDWLKCGVYVEPKIENFIKEIEKFEENKNKIKEFKLGRYDYYGMFMASIKKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXF8</accession>
    <name>N6VXF8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Myo-inositol-1-phosphate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_05733</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the myo-inositol 1-phosphate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592800.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXF8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01258"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1118"/>
    <dbReference type="OrthoDB" id="80661at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004512">
        <property type="term" value="F:inositol-3-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006021">
        <property type="term" value="P:inositol biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052199">
        <property type="entry name" value="MIPS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002587">
        <property type="entry name" value="Myo-inos-1-P_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013021">
        <property type="entry name" value="Myo-inos-1-P_Synthase_GAPDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43125">
        <property type="entry name" value="INOSITOL-3-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43125:SF1">
        <property type="entry name" value="INOSITOL-3-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01658">
        <property type="entry name" value="Inos-1-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015578">
        <property type="entry name" value="Myoinos-ppht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Myo-inositol-1-phosphate synthase GAPDH-like" evidence="3">
        <location>
            <begin position="212"/>
            <end position="317"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="105"/>
            <end position="160"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="382" mass="42574" checksum="0E48324CC793F420" modified="2013-06-26" version="1">MVNVIILGQGFVGSIFASGLEKIKAGEIEPYGVPLRDELQIKIKDINIVGSYDVDVNKVGKSVYDVAKHYDNNVPESLKDIEVKKGVHLNSLKNLPIKAEGLEDKMSLEDAINHLIDEWKEAKAEVFINVCTTEAFKTFNSREELEKAIKENNKERLTATQFYAYAISRYAKEVGGACFINAIPTLIANDPTYVELAKESKMVIFGDDGATGATPLTADLLAHLFQRNRYVLSIAQFNIGGNTDFLALTDVDRNKSKESTKSSIVEDILGYNAPHFIKPTGYLEPLGDKKFISLHIEYISFNGARDELIVNGRINDSPALAGLLVDLSRLGKIALDRKEYGTVYEVNAFYMKNPGPKEAKNIPRIIAYEKMRMWAGLKSIWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXG0</accession>
    <name>N6VXG0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05758</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592809.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXG0"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="78" mass="9164" checksum="9E1A7EA9D3D9F420" modified="2013-06-26" version="1">MYIYYLLIASSITVLYCILVSMLQNNFVTPTILIFFIILVLTLNIKRIYSLLKKLEIFFMVLVILGFGLALYKLYLPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXG4</accession>
    <name>N6VXG4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">4Fe-4S ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_05783</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592819.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXG4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01249"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1127"/>
    <dbReference type="OrthoDB" id="23478at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10549">
        <property type="entry name" value="MtMvhB_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF1">
        <property type="entry name" value="FERREDOXIN III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="7"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="72"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="116"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="146"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="225"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="265"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="6">
        <location>
            <begin position="296"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="320"/>
            <end position="362"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="397" mass="46127" checksum="EA0DA41475A925B0" modified="2013-06-26" version="1">MSFLGYIYEIKENIKDLLFAKSKKSYYIPPKRYRKIPPSVVYPEKCISCSACKESCPAFAIEMKYNNEHKKELPLIDEGSCIACANCVEVCPTGVLEIDKHRVETEDEPYIKPKYNYLLIDEEICVKCGNCKKVCPVEAIYKKEKKYYINIQNCISCKRCLDACPVNAIVILDEKTFKEKNRIFFDLKIKKEMNKLKYEEKIEKTPHIVNSLCILCRNCKEICFGEIDLEKKVVIKCVKCGYCLEVCPTSAIRVYKERIAKNKDSCYIINEDQCIGCRICYKVCEVNAIKISKETKLPYILPEKCVACGLCARECPVNAIKEVKKDMAMKEVEKRIIEDKIIEKIENELYNYMEEYSKILQEVEITAKKSLENEVKKRIKNEIKRIKDLKRGYNGDG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXK5</accession>
    <name>N6VXK5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">CDP-diacylglycerol--serine O-phosphatidyltransferase</fullName>
            <ecNumber evidence="4">2.7.8.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Phosphatidylserine synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">J422_05454</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>a CDP-1,2-diacyl-sn-glycerol + L-serine = a 1,2-diacyl-sn-glycero-3-phospho-L-serine + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16913"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57262"/>
            <dbReference type="ChEBI" id="CHEBI:58332"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="2.7.8.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Endomembrane system</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.8" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000041">
        <property type="protein sequence ID" value="ENN95867.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592535.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXK5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01400"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1065"/>
    <dbReference type="OrthoDB" id="221913at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003882">
        <property type="term" value="F:CDP-diacylglycerol-serine O-phosphatidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050324">
        <property type="entry name" value="CDP-alcohol_PTase-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004533">
        <property type="entry name" value="CDP-diaglyc--ser_O-PTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00473">
        <property type="entry name" value="pssA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3-PHOSPHATIDYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14269:SF61">
        <property type="entry name" value="CDP-DIACYLGLYCEROL--SERINE O-PHOSPHATIDYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="13" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 15 17" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 16" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="12"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="75"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="108"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="131"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="159"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000287"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN95867.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="194" mass="22037" checksum="5589F54E5B6D31D6" modified="2013-06-26" version="1">MRIFKLITVSDYVTILNAVSGILAILLNNYHFIYLAVIFDALDGYVARKTQTVSEFGKELDSLADVISFSIAPAYFFYLKHGLILLPIIYSICGMLRLARFNIIKTNNFIGLPIPAAALCMITLISISDYTVLNSLFLLLVSFLMISDFEYKKYFKNEIIILILISLILAIMNFPYLLMILLIIYIISPIIELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXM8</accession>
    <name>N6VXM8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2124 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05289</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95892.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592371.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXM8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01329"/>
    <dbReference type="OrthoDB" id="64681at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009183">
        <property type="entry name" value="UCP004962"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09897">
        <property type="entry name" value="DUF2124"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004962">
        <property type="entry name" value="UCP004962"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95892.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15615" checksum="CF2C156DE3AA7BB0" modified="2013-06-26" version="1" fragment="single">MKGISQLLRDFRRVVEENNIMSIAYAGIPGFCQPFAELFAYVIKNKENYFIPYTDLNNVKILTLKENIGMQMEDFGKIDYVDALVIFGGLAMPKFNVSEEEVRELILKLSPKITIGVGFMDIFKKVNWKIKFDYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXP6</accession>
    <name>N6VXP6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system Cms protein Csm5</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">CRISPR type III A-associated protein Csm5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_05259</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This subunit might be involved in maturation of a crRNA intermediate to its mature form.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Csm5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000035">
        <property type="protein sequence ID" value="ENN95912.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXP6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00739"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1027"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010173">
        <property type="entry name" value="CRISPR-assoc_Csm5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01899">
        <property type="entry name" value="cas_TM1807_csm5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38007">
        <property type="entry name" value="CRISPR SYSTEM CMS PROTEIN CSM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38007:SF1">
        <property type="entry name" value="CRISPR SYSTEM CMS PROTEIN CSM5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="7">
        <location>
            <begin position="3"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95912.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="379" mass="44929" checksum="5EB2C3148331EC0E" modified="2013-06-26" version="1">MLKCSLITPIFIGCGEEYTPLDYYIENNIAHIVDIEKALEVLGNLKSIDEISDYIVKKSIGNRVETNAKDILERVGLCAEDYVVYKIKSEIKEDSRTRVKKFIHQNNRKYIPGSSIKGAIRTAYLFNYYDGKISEIFKILDNKKIDNKGKEIERRAIGDIRDDFFKYLKISDSIKLEREEFKFILTKRWHIKKKKLTIPNYLEGMTKGEFIINLKVEEEFFKELNNRLNKNFNPKNDYEKFEIIKELCNNFSKTVVDFEIKRDLPIILKEFYEKLKKDIEKNDAIYINLGFEGGFLNKTIYPLLWKNDQNNILFHKIRQFFLDLYKPKGKINPRNPQYKLYQTWQNAKSYLDFPTTVGVYVKHNRISSPLGWIKLEKVN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXQ6</accession>
    <name>N6VXQ6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Succinate dehydrogenase flavoprotein subunit</fullName>
            <ecNumber evidence="6">1.3.5.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">sdhA</name>
        <name evidence="6" type="ORF">J422_05229</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.5.1" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000034">
        <property type="protein sequence ID" value="ENN95922.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592264.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXQ6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00304"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1021"/>
    <dbReference type="OrthoDB" id="23539at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008177">
        <property type="term" value="F:succinate dehydrogenase (quinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.700.10:FF:000002">
        <property type="entry name" value="L-aspartate oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.100">
        <property type="entry name" value="Fumarate reductase/succinate dehydrogenase flavoprotein-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.700.10">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003953">
        <property type="entry name" value="FAD-dep_OxRdtase_2_FAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037099">
        <property type="entry name" value="Fum_R/Succ_DH_flav-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015939">
        <property type="entry name" value="Fum_Rdtase/Succ_DH_flav-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030664">
        <property type="entry name" value="SdhA/FrdA/AprA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027477">
        <property type="entry name" value="Succ_DH/fumarate_Rdtase_cat_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004900">
        <property type="entry name" value="PRK06263.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE 2 FLAVOPROTEIN SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11632:SF51">
        <property type="entry name" value="SUCCINATE DEHYDROGENASE [UBIQUINONE] FLAVOPROTEIN SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00890">
        <property type="entry name" value="FAD_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02910">
        <property type="entry name" value="Succ_DH_flav_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000171">
        <property type="entry name" value="SDHA_APRA_LASPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46977">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56425">
        <property type="entry name" value="Succinate dehydrogenase/fumarate reductase flavoprotein, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="2 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD-dependent oxidoreductase 2 FAD-binding" evidence="4">
        <location>
            <begin position="4"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Fumarate reductase/succinate dehydrogenase flavoprotein-like C-terminal" evidence="5">
        <location>
            <begin position="416"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="3">
        <location>
            <position position="268"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR000171-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95922.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="528" mass="58930" checksum="51DB592F4C03462A" modified="2013-06-26" version="1">MITDILIIGGGGAALRAAVECKGDVIIAVKGLLGKSGCTVMAEGGYNAVFDPKDSFKMHFYDTIKGGCFINNPKLVEILVKNAPKELINLEKFGALFDRSGNKIAQRPFGGQSFPRTCYCGDRTGHEIIRALLEYLSRFERIKVLEDVMAIKLIVEDNRCYGAVFLDLISGNIFPIFAKATILATGGAGNIYKITSNAKQKTGDGFALAYDEGASLIDMEMVQFHPTGIVGKGILVTEAVRGEGGRLYNKFGERFMERYDERLELATRDVVARAIYREIIEGRGVNGGVYLDVTHLPKDVIERKLETTFKQFLRFGIDIRKEPMIVSPTAHHFMGGIKINERCETDISGLYACGEVAGGIHGANRLGGNALADTQVFGAIAGKEASKYAENCEIEVFDVDISYPSGDINPMELIDRLREIMWKYVSLIRNKEGLEKAKSEINEIGKYLDEINTSSPLYLQKYFELKNMITVSKLVIDSALYRKESRGAHYREDYPETKDEWRGNIIIKGNKIRFEKLEYDVDEYIKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXW4</accession>
    <name>N6VXW4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="14 15 16">Dihydroxy-acid dehydratase</fullName>
            <shortName evidence="15">DAD</shortName>
            <ecNumber evidence="14 15 16">4.2.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">ilvD</name>
        <name evidence="19" type="ORF">J422_05025</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Functions in the biosynthesis of branched-chain amino acids. Catalyzes the dehydration of (2R,3R)-2,3-dihydroxy-3-methylpentanoate (2,3-dihydroxy-3-methylvalerate) into 2-oxo-3-methylpentanoate (2-oxo-3-methylvalerate) and of (2R)-2,3-dihydroxy-3-methylbutanoate (2,3-dihydroxyisovalerate) into 2-oxo-3-methylbutanoate (2-oxoisovalerate), the penultimate precursor to L-isoleucine and L-valine, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:24809"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="11">
            <dbReference type="Rhea" id="RHEA:24810"/>
        </physiologicalReaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate = (S)-3-methyl-2-oxopentanoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:27694"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="EC" id="4.2.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="15">Binds 1 [2Fe-2S] cluster per subunit. This cluster acts as a Lewis acid cofactor.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13 15">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="12 15">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="15">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the IlvD/Edd family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.9" evidence="14 15 16"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95977.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592127.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXW4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00484"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.985"/>
    <dbReference type="OrthoDB" id="8674at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00057"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00061"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004160">
        <property type="term" value="F:dihydroxy-acid dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.30.80:FF:000001">
        <property type="entry name" value="Dihydroxy-acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.80">
        <property type="entry name" value="IlvD/EDD C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00012">
        <property type="entry name" value="IlvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042096">
        <property type="entry name" value="Dihydro-acid_dehy_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004404">
        <property type="entry name" value="DihydroxyA_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020558">
        <property type="entry name" value="DiOHA_6PGluconate_deHydtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056740">
        <property type="entry name" value="ILV_EDD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000581">
        <property type="entry name" value="ILV_EDD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037237">
        <property type="entry name" value="IlvD/EDD_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00110">
        <property type="entry name" value="ilvD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002068">
        <property type="entry name" value="PRK00911.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43661">
        <property type="entry name" value="D-XYLONATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43661:SF3">
        <property type="entry name" value="D-XYLONATE DEHYDRATASE YAGF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24877">
        <property type="entry name" value="ILV_EDD_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00920">
        <property type="entry name" value="ILVD_EDD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143975">
        <property type="entry name" value="IlvD/EDD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00886">
        <property type="entry name" value="ILVD_EDD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00887">
        <property type="entry name" value="ILVD_EDD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 15" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="3 15" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 15" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="7 15" id="KW-0408">Iron</keyword>
    <keyword evidence="8 15" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 15 19" id="KW-0456">Lyase</keyword>
    <keyword evidence="6 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase N-terminal" evidence="17">
        <location>
            <begin position="31"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="Dihydroxy-acid/6-phosphogluconate dehydratase C-terminal" evidence="18">
        <location>
            <begin position="357"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="15">
        <location>
            <position position="466"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="via carbamate group" evidence="15">
        <location>
            <position position="121"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="440"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="15">
        <location>
            <position position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00029304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00029490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="NCBIfam" id="TIGR00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF00920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF24877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ENN95977.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="550" mass="59439" checksum="EA7F4F17D4E773FA" modified="2013-06-26" version="1">MKSDSVKRGIERAPHRALLKACGYTDEEIERPFIGIANSFNEIVPGHIHLRELADAVKKGVYANGGTAFEFNTIAICDGIAMGHEGMKYSLPSREVIADSIECMAKAHGFDGLVLIPSCDKVVPGMIMGAIRAEIPFIVLTGGAMPPGEFRGKRYDLISVFEGIGAYKANKISEEELKEIENIACPGAGSCAGLFTANTMACLTEAMGLSLPYCATSLANTAEKMRIAKKTGYAIMEMIKKGITMDKILTKEAFENAIIVDLALGGSTNTTLHIPAIANEVEKGLITLDDFDKLSDKVPHIASLRPGGEHFIIDLHRAGGIPAVLKVLEEIINKECLTVSGKTVREIINNVKYIDHNVIRTVDNPVHKTAGLRILKGSLAPRGAVVKISAVSPKMYKFKGPARVFNSEEEAVKAILNSEIEEGEVIVIRYEGPASTGMREMLAPTSAICGMGLDEKVALITDGRFSGGSRGPCIGHVSPEAMAGGPIAVVEDGDMIEIDMINKRLDLCLSEEEIKERLTKWKKPEIKVRGYLRRYSKLVTSADEGAVLRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXY5</accession>
    <name>N6VXY5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CaCA family Na+/Ca+ antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_05125</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95997.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592159.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXY5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00464"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1005"/>
    <dbReference type="OrthoDB" id="142185at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005262">
        <property type="term" value="F:calcium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008273">
        <property type="term" value="F:calcium, potassium:sodium antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006874">
        <property type="term" value="P:intracellular calcium ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1420.30">
        <property type="entry name" value="NCX, central ion-binding region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004481">
        <property type="entry name" value="K/Na/Ca-exchanger"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004837">
        <property type="entry name" value="NaCa_Exmemb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044880">
        <property type="entry name" value="NCX_ion-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00367">
        <property type="entry name" value="calcium/sodium antiporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846:SF8">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YRBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10846">
        <property type="entry name" value="SODIUM/POTASSIUM/CALCIUM EXCHANGER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01699">
        <property type="entry name" value="Na_Ca_ex"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="68"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="104"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="156"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="226"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="254"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="279"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="4"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Sodium/calcium exchanger membrane region" evidence="6">
        <location>
            <begin position="161"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95997.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="297" mass="32446" checksum="17A66C69FE9EEE22" modified="2013-06-26" version="1">MLLGIIYFFIGLVLLYYGSDWFVVGGEKTAKFFNVSNFVIGATVIAFGTSVPEIVSSVYAALTKSSGIAVGNAIGSCVCNIGLILAISSIVYPIIIKKDQIKNILFHLFFVALTFILGVDGYNCVDGAILFLTFIFYIIWVLKNDKIEKKKEEEEINIVISLIFLIIGLISVLIGAKLFIDGAKMIAFLLHIPDYIIGFSLVAFGTSVPELMVSIAAAKRRLGGMILGNIIGSNIADIGGALAISSFFSYLPTANIQMVILLIMSILLYIFAKKSKITRFDGLILLILYIFAIKFLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VY31</accession>
    <name>N6VY31_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Lysine 2,3-aminomutase YodO family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_04815</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96032.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592020.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VY31"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00981"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.943"/>
    <dbReference type="OrthoDB" id="21308at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003739">
        <property type="entry name" value="Lys_aminomutase/Glu_NH3_mut"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00238">
        <property type="entry name" value="KamA family radical SAM protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30538:SF0">
        <property type="entry name" value="L-LYSINE 2,3-AMINOMUTASE AQ_1632-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30538">
        <property type="entry name" value="LYSINE 2,3-AMINOMUTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01070">
        <property type="entry name" value="PLP-dependent"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="298"/>
            <end position="445"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96032.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="587" mass="70006" checksum="8F407D0949D6CBB1" modified="2013-06-26" version="1">MFSILPELKEIFSKDDIEEIRESLFKYLNEFEYKVRNEGIIKNEIDRWLSLKAIQVFKNFISKEYEKIAGFSTLELMIKAYKGEIKNEAFLEEIKHLLLAISGKAKYSEGFLGEKLKDKIIDFSKLSGREAGKKRSEFLDEVYRVMKDYIKKYPCGLDKEVIERRRENKRLLMEFFGIDEEDWNDYKWQFKNIIKGKKGLEILKEMKFIDFPKEDFEVIEMAIEKGIPFGITPYYLHLFDFDKAYKYDLPIRRQVIPTRHYVEVMASKEKEEFDFMGELDTTPIDLVTRRYVTIAIIKPFESCPQICVYCQRNWMVKNFDEKAFSGWDKVEKALEWFAEHDSMIEILITGGDPFSLSDKAIERILNRIEDMDHVVGVRFGTRTLLTVPMRITENLLDILENFNDNKRVLIVTHAESAYEITPEVKETVKKINKRGINVYNQHVFHKFVSRRFENVALRIALKRAGIIPYYTFYPKGKYEHKEYLIPIARLCQEIKEEARLLPGTFRTDEAIFNVPRMGKLHMRSFQDRELIALKEDGSRVYLMHPWEKGIYKTKLYTYEDYPIKDYLDSLKKIGENIEDYWTIWYYY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYJ7</accession>
    <name>N6VYJ7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03583</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96197.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591245.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYJ7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01387"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.699"/>
    <dbReference type="OrthoDB" id="122708at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DsrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96197.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="115" mass="13528" checksum="D71135946B3D0113" modified="2013-06-26" version="1">MKTAFLIFNFLHKEQPNIPLMFHILLFAGEMKEKGDDVKIILEGEAVQWAKLLLENDKLKNHFEKVKDCFIVCEACAAMFNVKDEIKDHFKLENDLYGHISLKKYMDEGYRIISF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYK2</accession>
    <name>N6VYK2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Geranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="10">GGGP synthase</shortName>
            <shortName evidence="10">GGGPS</shortName>
            <ecNumber evidence="10">2.5.1.41</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">(S)-3-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Phosphoglycerol geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_03608</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C3 hydroxyl of sn-glycerol-1-phosphate (G1P). This reaction is the first ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>sn-glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = sn-3-O-(geranylgeranyl)glycerol 1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23404"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="EC" id="2.5.1.41"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the GGGP/HepGP synthase family. Group II subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.41" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96202.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591255.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYK2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01382"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.704"/>
    <dbReference type="OrthoDB" id="7409at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047294">
        <property type="term" value="F:phosphoglycerol geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02812">
        <property type="entry name" value="PcrB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.390">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00112">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038597">
        <property type="entry name" value="GGGP/HepGP_synthase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008205">
        <property type="entry name" value="GGGP_HepGP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010946">
        <property type="entry name" value="GGGP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050064">
        <property type="entry name" value="IGPS_HisA/HisF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01769">
        <property type="entry name" value="GGGP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01768">
        <property type="entry name" value="GGGP-family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003198">
        <property type="entry name" value="PRK04169.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003201">
        <property type="entry name" value="PRK04169.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235:SF22">
        <property type="entry name" value="GERANYLGERANYLGLYCERYL PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21235">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISF/H IGP SYNTHASE SUBUNIT HISF/H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01884">
        <property type="entry name" value="PcrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 10" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="6 10" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="8 10" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="166"/>
            <end position="172"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="197"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="219"/>
            <end position="220"/>
        </location>
        <ligand>
            <name>sn-glycerol 1-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57685"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96202.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="246" mass="27295" checksum="653DC5E10FA573ED" modified="2013-06-26" version="1">MKVEERLNRIIEEEGAIYFVLVDPENVNYEGLEKIKDYSDAILIGGSSGICELDYVVKEVKKITKLPTILFPGNVDNISKYADAILYMSLFNSLNPYWIITAPALGALKILKYNLEPIPTAYLCIEPAKKTSVGFVGDIKEIPQNKPKITAMYCLSAKFFGFRWAYLEAGSGAEYPINNETIKMAKKVSGINIIVGGGIKKPEIAYEKVLAGADGIVTGTLVEKDPKMIEKVYDAIKKAGREKKWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYL4</accession>
    <name>N6VYL4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 15">Archaeal Lon protease</fullName>
            <ecNumber evidence="15">3.4.21.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">ATP-dependent protease La homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">J422_03703</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Degrades polypeptides processively.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13 15">Homohexamer. Organized in a ring with a central cavity.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cell membrane</location>
            <topology evidence="1 15">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 15">Belongs to the peptidase S16 family. Archaeal LonB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.21.-" evidence="15"/>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96217.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591283.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYL4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01365"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.723"/>
    <dbReference type="OrthoDB" id="64652at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004663">
        <property type="entry name" value="Lon_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002078">
        <property type="entry name" value="Sigma_54_int"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00764">
        <property type="entry name" value="lon_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00158">
        <property type="entry name" value="Sigma54_activat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50045">
        <property type="entry name" value="SIGMA54_INTERACT_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 15" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 14 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0472">Membrane</keyword>
    <keyword evidence="7 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 14 15" id="KW-0645">Protease</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14 15" id="KW-0720">Serine protease</keyword>
    <keyword evidence="6 15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="11 15" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="113"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="domain" description="Sigma-54 factor interaction" evidence="16">
        <location>
            <begin position="123"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="17">
        <location>
            <begin position="452"/>
            <end position="635"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="546"/>
        </location>
    </feature>
    <feature type="active site" evidence="14">
        <location>
            <position position="589"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009579"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00026070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU369001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ENN96217.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="635" mass="70453" checksum="03FCD9C52D60769D" modified="2013-06-26" version="1">MFSVKFKTTEELPEPSPRLIDQVIGQDEAVRIVLSAVKNKRNVILLGDPGVGKSMIVKAVGEILQDIVDFKPYYVIAKPNLKNMERPIVEVIDGEYKEEKIEIPKVDFKSPNTFYLILVMIAAILLSEYLLRFVPEKYLLAAISITSLIVLIFGFVIILSAIIGSSRSGMGNTYSPADLKPVVLYECKKMPLVRASAYNVTRLLGDVKHCPLGGRPPLGTPPHKRIILGAIHEAHRGILYVDEIKTMPLEVQDYILTALQDKKLPISGRNPNSSGATVETNPIPCDFILIMSGNMDDVYNLRAPLLDRIDYKIVLRNKMDNTLENRNKLLQFIVQEIKNNNLNHLTYEACCEVVRVAQHLAGSKDKLTLRLRLLANILKMANDLAMGKEIEEILGNFDGKDYKPLTGKGKVLIEAKHIREVFERGIYSMEKQVAINYIKNFKRYKLITPNDSPKIGVIYGLAVLGEGGIGDVTKIIVQILDSKNPATHLLNISGDIAKHSITLASALSKKLIANHKLPLPKKDIDLTNKEIYIQFSQSYSKIDGDSATAAVCLAILSALLEIPLKQDFAITGSLDLSGNVLAVGGINEKIEAAKRYGFKRVIIPESNMIDVIEGEGIEIIPVKTLDEIIPLVFEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYP1</accession>
    <name>N6VYP1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphate transport system permease protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_03253</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the binding-protein-dependent transport system for phosphate; probably responsible for the translocation of the substrate across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96247.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590943.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYP1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01038"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.637"/>
    <dbReference type="OrthoDB" id="338493at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011864">
        <property type="entry name" value="Phosphate_PstC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051124">
        <property type="entry name" value="Phosphate_Transport_Permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02138">
        <property type="entry name" value="phosphate_pstC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30425">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30425:SF1">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PSTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="71"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="109"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="163"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="220"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="281"/>
            <end position="304"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="73"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96247.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="312" mass="34859" checksum="20C30C4F165EB284" modified="2013-06-26" version="1">MNFKKIFKKIDSFVIITFPAILIVFLIFITMLLSYFINAYPAIEKFGIDLFIKNIWIAAEEASREKYGLAAAIWGSVYTATIAVIFAIPIAICFAIFVNDYAPKWLKQILVIISDIMAGLPTIIYGIWGAFFLVPILKDTVMKILYEHFSFIPLFNYPPITGYSYFAAGILLGIMVTPFASAIIREAYAMIPQVYKEGLYALGCTKYEATKQLIKYISPAIVSGFILAFGRALGETVAVSLVIGNSFNLTYKLFAPGYTISSLIANQFGNANLYEYMLSTLYAAGLVLFIIGLVVNIIGVIYLMKWRKQISH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYT6</accession>
    <name>N6VYT6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TPR repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_02984</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96292.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590743.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYT6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00144"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.585"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13176">
        <property type="entry name" value="TPR_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="8"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="135"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="169"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="203"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="237"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96292.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="302" mass="35575" checksum="5409DADEEF3C7AAD" modified="2013-06-26" version="1">MEFLIEMCTNGNKLLKALEDIEEKLKKNPKNYDLLFKKFMILLGLKRFEEAIQCLDGIINYYGELPIIMLFKGSILYNLGRLKEARDCLKKVCKKHFFYVMIIFSHLGWVYYKLGEYDKVIKLCDLVLEISKDFYMAHVIKGQMYYLLGRYKEAIINFEKALKRNPEDCEVTYYLGDIYYRMGEFDKALKYLNKCIKSCPYDIRSLEKLVNIYILFGKYKKALEILKRIIELTPDDPLVYVKYGIILNKMKKYDEAIKYFDKALSINPTLVEALEGKALALEKLGKVEEAVKLYNKALELLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ12</accession>
    <name>N6VZ12_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Tyrosine protein kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02774</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96367.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590614.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ12"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00106"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.544"/>
    <dbReference type="OrthoDB" id="86092at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004672">
        <property type="term" value="F:protein kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052396">
        <property type="entry name" value="Meiotic_Drive_Suppr_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37171">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE YRZF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37171:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE YRZF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00069">
        <property type="entry name" value="Pkinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="1">
        <location>
            <begin position="23"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96367.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="204" mass="23443" checksum="8DB5C6BEBC00F88F" modified="2013-06-26" version="1">MLFKKIPNEIVDEKVLEKLKKKVKLVDAIGKGHRGVVFKGIYNNKTVAVKIPRTDGKNTILNEVNILKLLEPYNISPKVYDYSKDYLIMEYIEGEELKSVVEKLDKKNLIEVIEEVLKIALRLDSLNIEHKEIKGGRHFLVGEKVYIIDFDKAKKKKTTKNFTSAIALLFGENKVGKVVRNKLNLNEDDIKFIKRLAKIYKGSV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ68</accession>
    <name>N6VZ68_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription factor S</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Transcription elongation factor IIS/RNA polymerase subunit homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">J422_02574</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Induces RNA cleavage activity in the RNA polymerase. In its presence, the cleavage activity of the RNA polymerase truncates the RNA back to position +15 in a stepwise manner by releasing mainly dinucleotides from the 3'-end of the nascent RNA. The truncated RNAs are able to continue elongation. Involved in transcriptional proofreading and fidelity. Misincorporation of nucleotides during elongation of transcription leads to arrested elongation complexes which are rescued by TFS-promoted removal of a dinucleotide from the 3'-end. TFS is able to induce a cleavage resynthesis cycle in stalled elongation complexes (resulting from the next missing nucleotide or a reduced incorporation rate of a wrong nucleotide) preventing misincorporation and enabling proofreading in a post-incorporation manner. Pausing of elongation complexes is the main determinant of TFS-induced RNA cleavage.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 15">Belongs to the archaeal rpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96422.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590477.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ68"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00758"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.504"/>
    <dbReference type="OrthoDB" id="72957at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10511">
        <property type="entry name" value="Zn-ribbon_TFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000029">
        <property type="entry name" value="DNA-directed RNA polymerase subunit M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019761">
        <property type="entry name" value="DNA-dir_RNA_pol-M_15_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012164">
        <property type="entry name" value="Rpa12/Rpb9/Rpc10/TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006288">
        <property type="entry name" value="TFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001529">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001222">
        <property type="entry name" value="Znf_TFIIS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01384">
        <property type="entry name" value="TFS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11239:SF12">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02150">
        <property type="entry name" value="Zn_ribbon_RPB9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01096">
        <property type="entry name" value="Zn_ribbon_TFIIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005586">
        <property type="entry name" value="RNApol_RpoM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00661">
        <property type="entry name" value="RPOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00440">
        <property type="entry name" value="ZnF_C2C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01030">
        <property type="entry name" value="RNA_POL_M_15KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00466">
        <property type="entry name" value="ZF_TFIIS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51133">
        <property type="entry name" value="ZF_TFIIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="2" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 12 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="5 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="4 13 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIS-type" evidence="16">
        <location>
            <begin position="64"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="zinc finger region" description="C4-type" evidence="13">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="5"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00018272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00032962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00053900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR005586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR005586-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN96422.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="107" mass="12690" checksum="7A51E0A85FE3B875" modified="2013-06-26" version="1">MVKFCPKCNNIMLPKNGMLVCAVCGYVEEASEDSKKEYEYKEQLDKKKEITIIEGKEMETLPTTRAECPKCGHKEAYWWMQQTRCADEPETRFYKCKKCGHTWREYD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ99</accession>
    <name>N6VZ99_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_02120</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96447.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590224.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ99"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01173"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.417"/>
    <dbReference type="OrthoDB" id="372128at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058374">
        <property type="entry name" value="DUF8061"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040087">
        <property type="entry name" value="MJ0021-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288">
        <property type="entry name" value="BIOTIN SYNTHASE-RELATED PROTEIN, RADICAL SAM SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43288:SF1">
        <property type="entry name" value="GLYCYL-RADICAL ENZYME ACTIVATING ENZYME MJ0021-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26257">
        <property type="entry name" value="DUF8061"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01108">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="23"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96447.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="373" mass="43688" checksum="455ACD7BB5B30E8B" modified="2013-06-26" version="1">MRVESVKRYLKRNYNRLPEGCKYCIKGEKLVLFITGLCKNNCYYCPLSDKRRKKDVIYANERLINSVNEAIEEAKLCSSRGVGITGGDPLLKVDRTIKYLKALKNHFKEFHSHLYTVPESITEEKLKLLSEYLDEIRLHPTKIFNEGYDEEYIKFLLEKIELCKKYIKHVGVEIPSIPNMEKEILSLAYAIDGKADFMNINELEFSDTNYDELIKRGFEPKDDISNAIKGSEETALKVIKKFKGKMFIHYCPSVLKDAIQLKNRLINRAKNVAKPYEVITDEGLLLRGIMIFDKEKDMKEIIEILKDNEVEFEVKENKIYLNPFILEDLIKEMKRQRFPIKFSAYISEVYPTADALEVERIPLITKKLRLRRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZI5</accession>
    <name>N6VZI5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Pfpi family intracellular protease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01875</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase C56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96522.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590131.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZI5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00223"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.369"/>
    <dbReference type="OrthoDB" id="82036at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03135">
        <property type="entry name" value="GATase1_DJ-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006286">
        <property type="entry name" value="C56_PfpI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002818">
        <property type="entry name" value="DJ-1/PfpI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050325">
        <property type="entry name" value="Prot/Nucl_acid_deglycase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01382">
        <property type="entry name" value="PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094:SF12">
        <property type="entry name" value="PARKINSON DISEASE PROTEIN 7 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48094">
        <property type="entry name" value="PROTEIN/NUCLEIC ACID DEGLYCASE DJ-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01965">
        <property type="entry name" value="DJ-1_PfpI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51276">
        <property type="entry name" value="PEPTIDASE_C56_PFPI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DJ-1/PfpI" evidence="2">
        <location>
            <begin position="31"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008542"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96522.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="199" mass="22137" checksum="BD53A3CD127A97D4" modified="2013-06-26" version="1">MLRIIALIIIFILFSMCLDNHKSGESMEKGKILMVIAPKDFRDEELFIPMEVFKEHGYGVDVVSTQKGTCVGMLGGKIEVDKTIYEINPNDYIAIVIPGGIGSKEYLWNNEKLLELVREFYRENKTVAAICLSPVVLARAGVLKDKKATVFPTKEAIEELKKYGAEYVDEGVVIDGKIVTGKSPEYAKDFALAIIKTLG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZS9</accession>
    <name>N6VZS9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">APHP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01630</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589985.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZS9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00453"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.321"/>
    <dbReference type="OrthoDB" id="62381at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030246">
        <property type="term" value="F:carbohydrate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd08547">
        <property type="entry name" value="Type_II_cohesin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.680">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011635">
        <property type="entry name" value="CARDB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008965">
        <property type="entry name" value="CBM2/CBM3_carb-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022651">
        <property type="entry name" value="S_layer_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07705">
        <property type="entry name" value="CARDB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05124">
        <property type="entry name" value="S_layer_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49384">
        <property type="entry name" value="Carbohydrate-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CARDB" evidence="2">
        <location>
            <begin position="148"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="domain" description="CARDB" evidence="2">
        <location>
            <begin position="390"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="domain" description="S-layer protein outer" evidence="1">
        <location>
            <begin position="864"/>
            <end position="944"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="971" mass="108760" checksum="62C8F676130AA5DD" modified="2013-06-26" version="1" fragment="single">VFSSPEGQHIENISVEGCSIVINYKPIADIIVPDKINGELYKNLVFTTIIKPKENITIKSFEGYIIYDKKMLKLTNITSNVSIKYNDSNGMFIADNLNSTTPVEINFTFNIINVGNTTIELKNVKLADITGAYEIKNSNVTKVIIPGPDLVIENITLNQPIICYQNNSINVKIKNVGERNITDNFTVSLCADSQIVGKKIINGLNVGESKNITFDFMPIDIKNYTITAIVDSENKVKEINENNNYLVLTNIIATENPIVIKINPSKNLIKTDQTFVVNISLENISPKRPVKAIEGKIFYNKNVLKCENFSFLITPISENLTNISINDGYIYFSIVDGKINTSKPIAQAIFRAVDVGYSEISLGNLVVSDVNGYKFNKIITNNASIVVQGPNIKIASVSLPNKIYYRIPAEINITIVNDGHQDIINKSFDINLYADSETIGSITINNLKVGEKKTISFVWKPKELKNYVLVVLVDKYNIIKEENESDNKLVKIVKVNEIPIYVDTVKNNTTVKIYVRGVPIERNCSGYDVYIKFDNVNISNISAIGVYNYTIKNNIIFLTGYNFTKHGNFVIANISVINISNKSYIVIKNIKLSDGDGYPYNLVFVNNTLLPEKIKIALEGINLDSEVLNNTYIDKVKMEKIKDINLTIIPTTIDTLVIPKLENTSINITKYVIKKIKDIKDINNDLMNIKISSEKDIEKIISEIKVESVISLGFNITNITKKISKKVNNKIISEIKFNVSNLSKKGFSIIYIPIGHLKVETIKVDNKELRPLGDPNVNVSLGWFKIDNGILKITLVKDPIVNVLLVTELPTTTTYYSSGGGHHRAIEQYPGVAEDIKSEKIKELVYKSKVIVGNDVDLNLSAKYLKDVVDIINRSLEIKEDCILIGGPIANPVVKKWLWAFPVKVTNDYPGKNRGVIEKQIINGHIVILLAGSDRWGTKAAVEYFKTLDDIPDEPIFVEWKDGKAVKIEKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZX2</accession>
    <name>N6VZX2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TIM-barrel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01046</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589877.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZX2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00835"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.203"/>
    <dbReference type="OrthoDB" id="145053at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005270">
        <property type="entry name" value="tRNA_dU_NifR3-rel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00736">
        <property type="entry name" value="nifR3_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="243" mass="28865" checksum="AF09D7DD22AFC1B6" modified="2013-06-26" version="1">MIFLAPMAGITDGEFCLRYKNYFDAFVLGGYNLDKKTLEAAKLISNRRREFIIDLDKFNDYIRCEIKKVKKYGKVSVNVRFFNLEEAYDKLLTISKYADYLELNCHCRQREITSLGLGQELLKDKERLKYYLKNINFDIPIFLKIRLNYIPLNTLIRNLNYVRDYFDGLHIDCYYPNKPYPDINSLKIISEEFKDKEIIGNNSINSLERAKEMLKYSDHISVARAVLNNNISWIMKLRDYYGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZZ3</accession>
    <name>N6VZZ3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 7">16S rRNA aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.157</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_01181</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Aminocarboxypropyltransferase that catalyzes the aminocarboxypropyl transfer on pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA. It constitutes the last step in biosynthesis of the hypermodified N1-methyl-N3-(3-amino-3-carboxypropyl) pseudouridine (m1acp3-Psi).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-methionine = N(1)-methyl-N(3)-[(3S)-3-amino-3-carboxypropyl]pseudouridine in rRNA + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63296"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:16310"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="ChEBI" id="CHEBI:146234"/>
            <dbReference type="EC" id="2.5.1.157"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the TDD superfamily. TSR3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.157" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96662.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589902.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZZ3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00808"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.231"/>
    <dbReference type="OrthoDB" id="7441at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106388">
        <property type="term" value="F:rRNA small subunit aminocarboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000455">
        <property type="term" value="P:enzyme-directed rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01116">
        <property type="entry name" value="TSR3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007209">
        <property type="entry name" value="RNaseL-inhib-like_metal-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022968">
        <property type="entry name" value="Tsr3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007177">
        <property type="entry name" value="Tsr3_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002621">
        <property type="entry name" value="PRK02287.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426:SF0">
        <property type="entry name" value="18S RRNA AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20426">
        <property type="entry name" value="RIBOSOME BIOGENESIS PROTEIN TSR3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04068">
        <property type="entry name" value="Fer4_RLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04034">
        <property type="entry name" value="Ribo_biogen_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="4 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNase L inhibitor RLI-like possible metal-binding" evidence="9">
        <location>
            <begin position="1"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="16S/18S rRNA aminocarboxypropyltransferase Tsr3 C-terminal" evidence="8">
        <location>
            <begin position="41"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00014114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04034"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96662.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="168" mass="19640" checksum="3AE97ADDE5913275" modified="2013-06-26" version="1">MRLFIYHANQCNPKKCTSLKMAKLGKAIIIKNPYKIPKNSLILNPFAEKAISSEDRDIIEKFGITAFDCSWKEAESIFKKFRFKNERALPFLIAANPINYGKPFMLSTLEAFIASLYIVNLKNEAYELTKCFKWAETFININYELLERYSKAKNSKEIVEIQKEYMVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="59" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZZ6</accession>
    <name>N6VZZ6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="19">Bifunctional NAD(P)H-hydrate repair enzyme</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="19">Nicotinamide nucleotide repair protein</fullName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="19">ADP-dependent (S)-NAD(P)H-hydrate dehydratase</fullName>
                <ecNumber evidence="19">4.2.1.136</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="19">ADP-dependent NAD(P)HX dehydratase</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="19">NAD(P)H-hydrate epimerase</fullName>
                <ecNumber evidence="19">5.1.99.6</ecNumber>
            </recommendedName>
        </domain>
    </protein>
    <gene>
        <name evidence="18" type="primary">nnrE</name>
        <name evidence="17" type="synonym">nnrD</name>
        <name evidence="22" type="ORF">J422_01206</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 19">Bifunctional enzyme that catalyzes the epimerization of the S- and R-forms of NAD(P)HX and the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. This allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="17">Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ADP, which is converted to AMP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration.</text>
    </comment>
    <comment type="function">
        <text evidence="18">Catalyzes the epimerization of the S- and R-forms of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. This is a prerequisite for the S-specific NAD(P)H-hydrate dehydratase to allow the repair of both epimers of NAD(P)HX.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 18 19">
            <text>(6R)-NADHX = (6S)-NADHX</text>
            <dbReference type="Rhea" id="RHEA:32215"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:64075"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 18 19">
            <text>(6R)-NADPHX = (6S)-NADPHX</text>
            <dbReference type="Rhea" id="RHEA:32227"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:64077"/>
            <dbReference type="EC" id="5.1.99.6"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 17 19">
            <text>(6S)-NADHX + ADP = AMP + phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32223"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:64074"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17 19">
            <text>(6S)-NADPHX + ADP = AMP + phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:32235"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="4.2.1.136"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18 19">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
        <text evidence="18 19">Binds 1 potassium ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="17">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="17">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="18">Belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">In the C-terminal section; belongs to the NnrD/CARKD family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 19">In the N-terminal section; belongs to the NnrE/AIBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.136" evidence="19"/>
    <dbReference type="EC" id="5.1.99.6" evidence="19"/>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96667.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589907.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZZ6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00803"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.236"/>
    <dbReference type="OrthoDB" id="15148at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052855">
        <property type="term" value="F:ADP-dependent NAD(P)H-hydrate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052856">
        <property type="term" value="F:NAD(P)HX epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0110051">
        <property type="term" value="P:metabolite repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046496">
        <property type="term" value="P:nicotinamide nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01171">
        <property type="entry name" value="YXKO-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10260">
        <property type="entry name" value="YjeF N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01965">
        <property type="entry name" value="NADHX_dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01966">
        <property type="entry name" value="NADHX_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000631">
        <property type="entry name" value="CARKD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030677">
        <property type="entry name" value="Nnr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004443">
        <property type="entry name" value="YjeF_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036652">
        <property type="entry name" value="YjeF_N_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00196">
        <property type="entry name" value="yjeF_cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00197">
        <property type="entry name" value="yjeF_nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592:SF0">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12592">
        <property type="entry name" value="ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01256">
        <property type="entry name" value="Carb_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03853">
        <property type="entry name" value="YjeF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017184">
        <property type="entry name" value="Nnr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64153">
        <property type="entry name" value="YjeF N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51383">
        <property type="entry name" value="YJEF_C_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51385">
        <property type="entry name" value="YJEF_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 17 19" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11 18 19" id="KW-0413">Isomerase</keyword>
    <keyword evidence="22" id="KW-0418">Kinase</keyword>
    <keyword evidence="12 17 19" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 18 19" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="10 17 19" id="KW-0520">NAD</keyword>
    <keyword evidence="8 17 19" id="KW-0521">NADP</keyword>
    <keyword evidence="6 17 19" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 18 19" id="KW-0630">Potassium</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="22" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="YjeF N-terminal" evidence="21">
        <location>
            <begin position="29"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="domain" description="YjeF C-terminal" evidence="20">
        <location>
            <begin position="223"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="73"/>
            <end position="77"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <begin position="151"/>
            <end position="157"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="17">
        <location>
            <position position="418"/>
        </location>
        <ligand>
            <name>(6S)-NADPHX</name>
            <dbReference type="ChEBI" id="CHEBI:64076"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000013"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00000909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025153"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00048238"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRNR" id="PIRNR017184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="20">
        <source>
            <dbReference type="PROSITE" id="PS51383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ENN96667.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="476" mass="53471" checksum="D5E20D38CA504288" modified="2013-06-26" version="1">MFRDDDEIKIFYEIKEKIGEKEVISPKEMAIIDENAEYLGINRLMLMENAGRAVYEEIKDKEGEFIIFCGVGNNGGDGFVVARHLNKATVILLGKEDEIKTYEARENFKILKNLSIFGNIKIREIRHVNEICDIFERLKEGKYIIIDAMLGTGQRGELREPYKSVVSELNKLKERNKELFIVSVDVETGDLKIDLTVTFHKKKNLNKNAIIKDIGIPKEAEWIVGFGDIKALNLIENKHKGKNGKVLIIGGSKDYFGAPILAGLAALKIVDIVGILSVDKVIRKITHPEFILYSLEGDYVNNVDYALEVADGYDVVVIGNGLSVNEKTKDFVNEFINNYEKKLVIDADAIKVINYHKFDFREDVIFTPHKKEFNYIKFSSIPESTILLKGKKDIIFNSKNVKINLTGNKGMTKGGTGDVLAGLTGAIFAKNDPFISACSSAFINGYAADLLLKEKGSYYTPIDIINKIPNVLRLIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6W017</accession>
    <name>N6W017_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tRNA pseudouridine synthase Pus10</fullName>
            <ecNumber evidence="8">5.4.99.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">tRNA pseudouridine 54/55 synthase</fullName>
            <shortName evidence="8">Psi54/55 synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">pus10</name>
        <name evidence="11" type="ORF">J422_00936</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Responsible for synthesis of pseudouridine from uracil-54 and uracil-55 in the psi GC loop of transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>uridine(54) in tRNA = pseudouridine(54) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:57876"/>
            <dbReference type="Rhea" id="RHEA-COMP:10193"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8">
            <text>uridine(55) in tRNA = pseudouridine(55) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42532"/>
            <dbReference type="Rhea" id="RHEA-COMP:10101"/>
            <dbReference type="Rhea" id="RHEA-COMP:10102"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.25"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the pseudouridine synthase Pus10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.25" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589857.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W017"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00587"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.184"/>
    <dbReference type="OrthoDB" id="10348at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160148">
        <property type="term" value="F:tRNA pseudouridine(55) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.2510:FF:000001">
        <property type="entry name" value="tRNA pseudouridine synthase Pus10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3190:FF:000001">
        <property type="entry name" value="tRNA pseudouridine synthase Pus10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01893">
        <property type="entry name" value="Pus10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005912">
        <property type="entry name" value="Pus10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039894">
        <property type="entry name" value="Pus10-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048741">
        <property type="entry name" value="Pus10-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055174">
        <property type="entry name" value="Pus10_THUMP_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01213">
        <property type="entry name" value="pseudo_Pus10arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21568:SF0">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE PUS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21238">
        <property type="entry name" value="Pus10_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22023">
        <property type="entry name" value="Pus10_THUMP_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 8" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="THUMP" evidence="10">
        <location>
            <begin position="76"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="251"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="315"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="387"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00058132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="434" mass="50671" checksum="1B18F95F3F727AE4" modified="2013-06-26" version="1">MMYEILKKYPLCDRCFGRLFAKLLHATNIERGRALKIYKLLEIEKKINKGEADENDYELLKAILRSGCNKTRLNMEIKKESCPWCRGIFEKDKIERLLNKAINLLKEYDFNTFLIGTHLPEDIKELEEEIKTEYMESIKQEFGREFGKLLAIRLNKQPDKENPDIVVHINPYTEEIYLQVNPLFIKGRYRKLVRGISQTIWLCGKCKGKGCEICNYTGKKYPSSVEEIIAKPIMEATKGKDEKFHGAGREDVDVRMLGDGRPFIIEIKEPKIRNIDLKKIEEEINKDGRVEVLNLSFAKRKDVIAYKNTPHKKTYRALVECSEKVSEEDLKKLERELENRIIKQRTPKRVLHRRADLERIRKVYKVKTNKVDDNHFEMIIYCDGGLYIKELISGDDGRTKPSVSSILNKPCICKELDVLKVHDNETGDINGRNE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6W031</accession>
    <name>N6W031_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">4Fe-4S ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00681</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589812.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W031"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00899"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.131"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13560">
        <property type="entry name" value="HTH_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="19"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="2">
        <location>
            <begin position="116"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="155" mass="17828" checksum="C55F8212D86D1ADF" modified="2013-06-26" version="1">MPEHILSGVKAIVAMKLRRKGLTQEEIAELLKCNRSAISHYLTGKYPKEKVIRVARAIESLSPREGVKIIKNLIEDKELIKNLIKNLYDIEIYWDENKCLFCGSCLICEAISQEGMKIKIDKDKCWLCLKCLELCPANALILKRENYDSANKRVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6W048</accession>
    <name>N6W048_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein eL42</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 6" type="primary">rpl44e</name>
        <name evidence="6" type="ORF">J422_00811</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the eukaryotic ribosomal protein eL42 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589832.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W048"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00873"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.160"/>
    <dbReference type="OrthoDB" id="52456at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.450.80:FF:000001">
        <property type="entry name" value="60S ribosomal protein L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01476">
        <property type="entry name" value="Ribosomal_L44e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000552">
        <property type="entry name" value="Ribosomal_eL44"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053708">
        <property type="entry name" value="Ribosomal_LSU_eL42"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004425">
        <property type="entry name" value="PRK05767.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10369">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L36A/L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00935">
        <property type="entry name" value="Ribosomal_L44"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01172">
        <property type="entry name" value="RIBOSOMAL_L44E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="4" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="4">
        <location>
            <begin position="11"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU000666"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="95" mass="10977" checksum="FE920EC7E5E0F886" modified="2013-06-26" version="1">MKIPKKIRRYCPYCKKHTIHIVEKAKKGKASELTWGQRQFRRVTAGYGGFPRPKPEGRSKPVKKIDLRFKCTVCGKIHTKANGCFRAGKFEIVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6W0A1</accession>
    <name>N6W0A1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00050</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589703.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W0A1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01157"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.10"/>
    <dbReference type="OrthoDB" id="65448at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="258" mass="30416" checksum="E0603C205F0E7915" modified="2013-06-26" version="1">MILEKSKIEVMERFIFILEILNQHVAGVDEKVIIRHLLEYLEKGNMLDEDILPIANKILEIAKKSGNFEIKREVKLLLFGEKEKLTKAKKEKIRKIIDILELLKGYIENKPYKDFGDRLILNLIKLKILRLDDGVILDYKTEVRSLANLALNIGSYELKSEIENYLLGRKKRKLDEKFLEKKRLLLETLEMVKKYIESKEYKSAIDYEALFIITLKTYRIEENILNNFDSEINSILNIAKKVGDYKLRERINIIKVSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6W0E6</accession>
    <name>N6W0E6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(4)-bis(aminopropyl)spermidine synthase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589753.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W0E6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01097"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.66"/>
    <dbReference type="OrthoDB" id="358909at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014435">
        <property type="entry name" value="BpsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002723">
        <property type="entry name" value="BpsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051720">
        <property type="entry name" value="rRNA_MeTrfase/Polyamine_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290:SF0">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01861">
        <property type="entry name" value="BpsA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005895">
        <property type="entry name" value="UCP005895_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="N(4)-bis(aminopropyl)spermidine synthase C-terminal" evidence="1">
        <location>
            <begin position="127"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="384" mass="44074" checksum="8BB153B1B931FA6B" modified="2013-06-26" version="1">MIIGNIGRGKKEINEDDKFNIILDKIAKKVNVSEGRRAVEDIIRIIFREQPISTKKIAQKTRLPLPIVAKVRAMLEREKILKRNKGAILTDLGEKLAKEYLKLRYRGSLICDKCKGRGIILDEFFKEILNKAEKYFKKRPSPNTLIDQSFATLETSVYRVALMCERGDLEGRRIMFVGDDDLTSIPAALTGFADEIAVVDIDERILRIIEEFSKEEDVKIKIVKHDLRKPLPNDLKDSFDTISTDPPYTLNGLRLFLSRGIEALGKEGVAYLSYSHKPIEEFLLVQKAINEMNFVISELIPNFNYYEGSEIIANTTFIARLIGKDLKPNIGDTEKIYTGLVKPVIRYYKCLKCGKIYKLLNERIEDLKCSCGSRKFRMVKREKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTE0</accession>
    <name>N6UTE0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_07157</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000077">
        <property type="protein sequence ID" value="ENN95564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004595134.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000077.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTE0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01003"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1383"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861:SF6">
        <property type="entry name" value="METHYLTRANSFERASE TYPE 11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43861">
        <property type="entry name" value="TRANS-ACONITATE 2-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="30"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="226" mass="26123" checksum="E1A78B90C37ACB8F" modified="2013-06-26" version="1">MKHYREIDKILFLKALYFLKNKGFNPKNMVDIGCGDGHLTLKIKKVFNIPINNVYGIEYDKNLVISCKEKGIKCFLLDLNKDKIPFEDNSVDLITANQVLEHIFEIDDLLIEIKRILSPNGYFVVSTPNLAALHERISLLLGYNPSTWHTAKIQIGLRRGEPTNNREHVNGFTIKGLKYLLEYYGFKPVKLFVGKVFIKDDLYIPFLEKIFPSLALTQCWICKVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTI0</accession>
    <name>N6UTI0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-phospho-L-lactate transferase</fullName>
            <ecNumber evidence="3">2.7.8.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">EPPG:FO PEP transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cofD</name>
        <name evidence="4" type="ORF">J422_06852</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the transfer of the 2-phospholactate moiety from (2S)-lactyl-2-diphospho-5'-guanosine to 7,8-didemethyl-8-hydroxy-5-deazariboflavin (FO) with the formation of oxidized coenzyme F420-0 and GMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(2S)-lactyl-2-diphospho-5'-guanosine + 7,8-didemethyl-8-hydroxy-5-deazariboflavin = oxidized coenzyme F420-0 + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:63444"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:59435"/>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
            <dbReference type="ChEBI" id="CHEBI:59907"/>
            <dbReference type="EC" id="2.7.8.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CofD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.28" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95619.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594246.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTI0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01077"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1329"/>
    <dbReference type="OrthoDB" id="59563at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043743">
        <property type="term" value="F:LPPG:FO 2-phospho-L-lactate transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07186">
        <property type="entry name" value="CofD_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.240">
        <property type="entry name" value="CofD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10680">
        <property type="entry name" value="CofD-like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01257">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002882">
        <property type="entry name" value="CofD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038136">
        <property type="entry name" value="CofD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010115">
        <property type="entry name" value="FbiA/CofD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01819">
        <property type="entry name" value="F420_cofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43007">
        <property type="entry name" value="2-PHOSPHO-L-LACTATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43007:SF1">
        <property type="entry name" value="2-PHOSPHO-L-LACTATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01933">
        <property type="entry name" value="CofD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142338">
        <property type="entry name" value="CofD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3 4" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>7,8-didemethyl-8-hydroxy-5-deazariboflavin</name>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>7,8-didemethyl-8-hydroxy-5-deazariboflavin</name>
            <dbReference type="ChEBI" id="CHEBI:59904"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01257"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95619.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="307" mass="34824" checksum="EBA3F5C0D1146F59" modified="2013-06-26" version="1">MIIILTVLSGGTGTPKLLQGLKRFLSEELNIIVNTAEDTWINDLYVSPDVDTVLYTLADLINEDVWYGRRDDTFTTHETLKAFGIDELLRIGDKDRALKMHKAYYLKKGYKLCEIIDMERELFNIKAKVLPMSDDRVETKILTKLNGKLDLLKFHDFWVKRKGNVDVIDVIYENITYAKPCEKALRAIEKSKAIIIGPSNPITSIGPILAFDEIREGISKKKTIVVSPIVGNSPISGPAGKLMRAKGYDSSIYGIYEFYKDLVDILIIDISDKDKAKDFDCEVITTNIIMRTLEDKLRLAKEILELV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU28</accession>
    <name>N6UU28_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">ABC transporter ATP-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cbiO</name>
        <name evidence="12" type="ORF">J422_05539</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="function">
        <text evidence="9">Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000042">
        <property type="protein sequence ID" value="ENN95859.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592611.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU28"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00748"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1081"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000224">
        <property type="entry name" value="Energy-coupling factor transporter ATP-binding protein EcfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005876">
        <property type="entry name" value="Co_trans_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050095">
        <property type="entry name" value="ECF_ABC_transporter_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01166">
        <property type="entry name" value="cbiO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553:SF24">
        <property type="entry name" value="ENERGY-COUPLING FACTOR TRANSPORTER ATP-BINDING PROTEIN ECFA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553">
        <property type="entry name" value="HEAVY METAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 10 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="4"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU364103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95859.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="288" mass="32187" checksum="6E15E57E88AA087C" modified="2013-06-26" version="1">MYVLETRDLYFSYPDGTEVLKGINFKAKKGEMIALLGPNGVGKTTLFLHFNGILKPKRGEVLIKGKPIRYDKKGLMEVRRTVGIVFQNPDDQIFAPTVKEDVAFGPLNLGLPKEEVERRVKEALKAVGMEGYEDKPPHLLSGGQKKRVAIAGILAMEPEIIILDEPTAGLDPLGASKIMKLLYDLNKKGITIIISTHDVDLVPVYADRVYLMYGGKILKEGTPKDVFSDVETIRKANLRLPRVAHLVEILNKKDKKNIKMGYTIGEVRRNLLEALNCDNHEKNLGDVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUV2</accession>
    <name>N6UUV2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">3,4-dihydroxy-2-butanone 4-phosphate synthase</fullName>
            <shortName evidence="6 7">DHBP synthase</shortName>
            <ecNumber evidence="6 7">4.1.99.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ribB</name>
        <name evidence="8" type="ORF">J422_04058</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes the conversion of D-ribulose 5-phosphate to formate and 3,4-dihydroxy-2-butanone 4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>D-ribulose 5-phosphate = (2S)-2-hydroxy-3-oxobutyl phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18457"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="4.1.99.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6 7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="6 7">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="6 7">Binds 2 divalent metal cations per subunit. Magnesium or manganese.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6 7">Cofactor biosynthesis; riboflavin biosynthesis; 2-hydroxy-3-oxobutyl phosphate from D-ribulose 5-phosphate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 7">Belongs to the DHBP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.99.12" evidence="6 7"/>
    <dbReference type="EMBL" id="APMM01000026">
        <property type="protein sequence ID" value="ENN96129.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591529.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUV2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00725"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.795"/>
    <dbReference type="OrthoDB" id="25735at2157"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00399"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008686">
        <property type="term" value="F:3,4-dihydroxy-2-butanone-4-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00180">
        <property type="entry name" value="RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000422">
        <property type="entry name" value="DHBP_synthase_RibB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00506">
        <property type="entry name" value="ribB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327:SF46">
        <property type="entry name" value="3,4-DIHYDROXY-2-BUTANONE 4-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21327">
        <property type="entry name" value="GTP CYCLOHYDROLASE II-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00926">
        <property type="entry name" value="DHBP_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 6 7" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 6 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6 7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="23"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="159"/>
            <end position="163"/>
        </location>
        <ligand>
            <name>D-ribulose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="162"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="site" description="Essential for catalytic activity" evidence="6">
        <location>
            <position position="145"/>
        </location>
    </feature>
    <feature type="site" description="Essential for catalytic activity" evidence="6">
        <location>
            <position position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003843"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96129.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="223" mass="25534" checksum="2B0AE8B100D0F49E" modified="2013-06-26" version="1">MIEKAIKAFKNGEIVLIYDADDREGETDMVIASQFITPEVIRRMRKDAGGLICTAIHHDICSSLDLPYLVDIFSYASEKFRILRELYPNDIPYDEKSSFSITINHRKTFTGITDRDRAFTIKKLVELVNEKRYNDFGKEFRSPGHVILLRASEGLVKNRQGHTEMSVALAELSNLTPIATICEMLGDDGYAMSKSEAKRYAEKYNLIFIEGEELINYYLEKKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUX1</accession>
    <name>N6UUX1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Homoserine dehydrogenase</fullName>
            <ecNumber evidence="4">1.1.1.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">J422_03818</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the homoserine dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.3" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96154.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591357.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUX1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00395"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.748"/>
    <dbReference type="OrthoDB" id="4488at2157"/>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00063"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00465"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004412">
        <property type="term" value="F:homoserine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000005">
        <property type="entry name" value="Homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000554">
        <property type="entry name" value="Homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001342">
        <property type="entry name" value="HDH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019811">
        <property type="entry name" value="HDH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022697">
        <property type="entry name" value="HDH_short"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004912">
        <property type="entry name" value="PRK06270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004976">
        <property type="entry name" value="PRK06349.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43331">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43331:SF1">
        <property type="entry name" value="HOMOSERINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00742">
        <property type="entry name" value="Homoserine_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036497">
        <property type="entry name" value="HDH_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01042">
        <property type="entry name" value="HOMOSER_DHGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="8 14" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Aspartate/homoserine dehydrogenase NAD-binding" evidence="13">
        <location>
            <begin position="7"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="Homoserine dehydrogenase catalytic" evidence="12">
        <location>
            <begin position="152"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10">
        <location>
            <position position="219"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="7"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>NADPH</name>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>L-homoserine</name>
            <dbReference type="ChEBI" id="CHEBI:57476"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013376"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR036497-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN96154.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="334" mass="36439" checksum="A9F5BEC36E61602E" modified="2013-06-26" version="1">MRIIIIGFGVIGRGLAKVLYKKREFLRKNYGDFNVVAIADRSGAIIGEDIDLKRAIEVKEKSGKIINYPGIEKSSLEVIKDVDADVVVEVTPSNLDTGEPARTHIIESFKHGKHVVTANKGPLALYFKELMETAKRYNKIFKFEASVGGAMPIINLAKETLAGNKIISIRGILNGTTNYILTKMEEGLDFETVLKEAKELGIAETNPYQDISGLDTAAKIVILANAIMGLNKTIKDVKVRGIEKITPEALFLANKRGYTIKLIGEIVNGYLIVEPKLIPINSPLNVKGTLNVAMFETDLAKEVVVVGRGAGAEETASAILSDLISIKKMIENES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUY3</accession>
    <name>N6UUY3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03903</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96169.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591387.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUY3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00411"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.765"/>
    <dbReference type="OrthoDB" id="59569at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96169.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="79" mass="9488" checksum="A41D319309F6EE7C" modified="2013-06-26" version="1">MDYSFIKPIFREKIIDIDKGSLKTKRKFAFLLEKGDVLLEKREFYANDEVEVVDNYQITDSNRPKEKIKLYIIEDIIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV28</accession>
    <name>N6UV28_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Imidazole glycerol phosphate synthase subunit HisH</fullName>
            <ecNumber evidence="12">4.3.2.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">IGP synthase glutaminase subunit</fullName>
            <ecNumber evidence="12">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">IGP synthase subunit HisH</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">ImGP synthase subunit HisH</fullName>
            <shortName evidence="12">IGPS subunit HisH</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 15" type="primary">hisH</name>
        <name evidence="15" type="ORF">J422_03713</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisH subunit catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the synthesis of IGP and AICAR. The resulting ammonia molecule is channeled to the active site of HisF.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 17">
            <text>5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate + 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58278"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="4.3.2.10"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 12">Heterodimer of HisH and HisF.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.10" evidence="12"/>
    <dbReference type="EC" id="3.5.1.2" evidence="12"/>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96219.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591287.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV28"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01363"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.725"/>
    <dbReference type="OrthoDB" id="33401at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00010"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000107">
        <property type="term" value="F:imidazoleglycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01748">
        <property type="entry name" value="GATase1_IGP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000009">
        <property type="entry name" value="Imidazole glycerol phosphate synthase subunit HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00278">
        <property type="entry name" value="HisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010139">
        <property type="entry name" value="Imidazole-glycPsynth_HisH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01855">
        <property type="entry name" value="IMP_synth_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42701:SF1">
        <property type="entry name" value="IMIDAZOLE GLYCEROL PHOSPHATE SYNTHASE SUBUNIT HISH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000495">
        <property type="entry name" value="Amidotransf_hisH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 12" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="15" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="8 12" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="14">
        <location>
            <begin position="15"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="12 13">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="12 13">
        <location>
            <position position="176"/>
        </location>
    </feature>
    <feature type="active site" evidence="12 13">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000495-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN96219.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="195" mass="22038" checksum="A5B13F1B84769C7C" modified="2013-06-26" version="1">MIAIIDYNAGNLRSIQKAVEMFDKAIITNKKEDILSADKVILPGVGNFGSAVNHLEPLKDAIYKIIDDGIPFLGICLGMQILFERSEEKKDAKGFGIIKGDVVKFRNVKIPHMGWNTVKIVKDCILFENIKDNSYFYFVHSYYVKPYEDVVVGETEYGVVFPSVINKNNIFATQFHPEKSGKLGLKVIENFIELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVD3</accession>
    <name>N6UVD3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF63 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03104</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96314.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590770.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVD3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00167"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.609"/>
    <dbReference type="OrthoDB" id="84937at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002749">
        <property type="entry name" value="DUF63"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40700:SF1">
        <property type="entry name" value="DUF63 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40700">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF63 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01889">
        <property type="entry name" value="DUF63"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="25"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="87"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="143"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="176"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="210"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96314.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="267" mass="30517" checksum="5D9BBCD0F3131709" modified="2013-06-26" version="1">MLDKVYEFIYKYYIEPAEIGSGYNIIQEITYGAILAISLYLFYKALIRLNVKIDEKFAIPSIVFSILIALIRALVDLGYIERSFLTITPGIVFVIGGLFIITILTTAYVFKEKYYKVSAVIGFVIFLYFFSIFLTHIHHLDALIYVAMLVAVIYISIFYIDRILKLNILQNKIDSYAIIGQAIDASATAVGLAFYGYWEQHPIPRFFMEHFGAFSFIPIKILAVLLALYVVNREVEDENIKNIIKLCIMALGMAPGMRDLFRMIMGV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVP5</accession>
    <name>N6UVP5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Chorismate mutase</fullName>
            <ecNumber evidence="4">5.4.99.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_02524</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.5" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96414.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590461.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVP5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00767"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.494"/>
    <dbReference type="OrthoDB" id="64249at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004106">
        <property type="term" value="F:chorismate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046417">
        <property type="term" value="P:chorismate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009697">
        <property type="term" value="P:salicylic acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.59.10">
        <property type="entry name" value="Chorismate mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036263">
        <property type="entry name" value="Chorismate_II_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051331">
        <property type="entry name" value="Chorismate_mutase-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036979">
        <property type="entry name" value="CM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002701">
        <property type="entry name" value="CM_II_prokaryot"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004925">
        <property type="entry name" value="PRK06285.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38041">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38041:SF1">
        <property type="entry name" value="CHORISMATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01817">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00830">
        <property type="entry name" value="CM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48600">
        <property type="entry name" value="Chorismate mutase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51168">
        <property type="entry name" value="CHORISMATE_MUT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chorismate mutase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="4"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96414.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="95" mass="11315" checksum="DE9C3A323260CFCE" modified="2013-06-26" version="1">MKDLKKLREKIDEIDENILKLIAERNRLAKDIAEAKKALGLEIDDPKREKHIYEKIKRLCNCYNIDEDMAIKIFKILIEHNKKLQKKHLKGENYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVQ9</accession>
    <name>N6UVQ9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ketol-acid reductoisomerase (NADP(+))</fullName>
            <shortName evidence="11">KARI</shortName>
            <ecNumber evidence="11">1.1.1.86</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Acetohydroxy-acid isomeroreductase</fullName>
            <shortName evidence="11">AHIR</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Alpha-keto-beta-hydroxylacyl reductoisomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">ilvC</name>
        <name evidence="15" type="ORF">J422_02325</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NAD(+) = (2S)-2-acetolactate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:30627"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.383"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.383"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R)-2,3-dihydroxy-3-methylbutanoate + NADP(+) = (2S)-2-acetolactate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22068"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49072"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP(+) = (S)-2-ethyl-2-hydroxy-3-oxobutanoate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13493"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49256"/>
            <dbReference type="ChEBI" id="CHEBI:49258"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.86"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 12">Belongs to the ketol-acid reductoisomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.86" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96424.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590328.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVQ9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00344"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.457"/>
    <dbReference type="OrthoDB" id="6064at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00056"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00060"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004455">
        <property type="term" value="F:ketol-acid reductoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000023">
        <property type="entry name" value="Ketol-acid reductoisomerase (NADP(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.240.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00435">
        <property type="entry name" value="IlvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013023">
        <property type="entry name" value="KARI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000506">
        <property type="entry name" value="KARI_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013116">
        <property type="entry name" value="KARI_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014359">
        <property type="entry name" value="KARI_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00465">
        <property type="entry name" value="ilvC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004017">
        <property type="entry name" value="PRK05479.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009940">
        <property type="entry name" value="PRK13403.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21371:SF1">
        <property type="entry name" value="KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01450">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07991">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000116">
        <property type="entry name" value="IlvC_gammaproteo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51851">
        <property type="entry name" value="KARI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51850">
        <property type="entry name" value="KARI_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 11 12" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="15" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 11 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0521">NADP</keyword>
    <keyword evidence="7 11 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KARI N-terminal Rossmann" evidence="13">
        <location>
            <begin position="1"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="domain" description="KARI C-terminal knotted" evidence="14">
        <location>
            <begin position="182"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="24"/>
            <end position="27"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="190"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="194"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="226"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="251"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00050504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00052344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN96424.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="328" mass="36679" checksum="993D6A11CE7BC238" modified="2013-06-26" version="1">MKIYYDNEVSLDAVKDKVIAVIGYGSQGRAQALNMRDSGLNVILGLRPDGRSWKKAIEDNMNVKTIEEAAKEADIIHMLIPDEVQPIVYKKYIEKHLTEGKTLSFSHGYNIHYGLIIPPDNVNVTMVAPKAPGVKVRETYLEGFGVPGLVAVEQDYTGNALDIALGMAKAIGLTRVGVIKTTFKEETETDLFGEQVVLCGGVTELIKAAFETLVEAGYSPEMAYFETCHELKLIVDLIYQRGLEGMWQSVSNTAEYGGLTRRKRVINEESRKAMKEILKEIQEGDFAKEWSLENQAGAVKLNALRRLEREHLIEKVGKRLRKMCGLEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVU7</accession>
    <name>N6UVU7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Tetrahydromethanopterin S-methyltransferase subunit A</fullName>
            <ecNumber evidence="3">2.1.1.86</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02155</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96454.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590234.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVU7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01179"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.423"/>
    <dbReference type="OrthoDB" id="64662at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013347">
        <property type="entry name" value="MeTrfase_F_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02507">
        <property type="entry name" value="MtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009776">
        <property type="entry name" value="PRK13275.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09472">
        <property type="entry name" value="MtrF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="domain" description="Tetrahydromethanopterin S-methyltransferase F subunit" evidence="2">
        <location>
            <begin position="6"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96454.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="71" mass="7708" checksum="69B638532E38C405" modified="2013-06-26" version="1">MSEIEGIPKIVKPDLEYCDKMLERLEYKVGLITRDLGLSSGIHTKSTSGFIIGAALAIVLVGIPIIIKILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVZ7</accession>
    <name>N6UVZ7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Carbon monoxide dehydrogenase iron sulfur subunit CooF1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_01730</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96499.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590104.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVZ7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00197"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.340"/>
    <dbReference type="OrthoDB" id="2837at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050954">
        <property type="entry name" value="ET_IronSulfur_Cluster-Binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177">
        <property type="entry name" value="PROTEIN NRFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177:SF3">
        <property type="entry name" value="PROTEIN NRFC HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12798">
        <property type="entry name" value="Fer4_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="53"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96499.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="132" mass="14896" checksum="D54F4CDEA6FE3F3E" modified="2013-06-26" version="1">MIDIKKCVGCRRCEMSCPINGIIFLDYPIKCFHCEKAPCLEVCPENAIERINNKVVIIKDKCIGCGLCALACPFGAIRMGKVAMKCNGCYKLEKEICKTVCPTGAINDLENIINEKLKMSNERYRIIHLYSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6UW67</accession>
    <name>N6UW67_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">50S ribosomal protein L19e</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpl19e</name>
        <name evidence="7" type="ORF">J422_01505</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589962.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UW67"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00429"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.296"/>
    <dbReference type="OrthoDB" id="11624at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1200.240:FF:000003">
        <property type="entry name" value="50S ribosomal protein L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1200.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039547">
        <property type="entry name" value="Ribosomal_eL19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000196">
        <property type="entry name" value="Ribosomal_eL19_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057260">
        <property type="entry name" value="Ribosomal_L19e_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006343">
        <property type="entry name" value="PRK08570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10722">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25476">
        <property type="entry name" value="Ribosomal_L19e_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01416">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL19" evidence="6">
        <location>
            <begin position="1"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="1"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01416"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="95" mass="11217" checksum="1D90E94B53FA0508" modified="2013-06-26" version="1" fragment="single">KKGISNVRVKKLKEQKKKGRRRGPGSRRGAKGARTPQKERWMATIRALRKTLKHLRDEGKIDTKTYRKLYRMAKGGAFRSRAHLFLYMKEHEILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWB4</accession>
    <name>N6UWB4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Energy-converting hydrogenase B subunit J</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01490</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000010">
        <property type="protein sequence ID" value="ENN96594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589959.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWB4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01032"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.293"/>
    <dbReference type="OrthoDB" id="82319at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="72" mass="8180" checksum="F90AB376F858556B" modified="2013-06-26" version="1">MISLLYPSVLSFIVGFIVGIKYREKIRNKLLIIILSIVIAYFLGSFPYYNLPISFSYVSAVLGVIFGNMVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWC1</accession>
    <name>N6UWC1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Peptidyl-tRNA hydrolase</fullName>
            <ecNumber evidence="3">3.1.1.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_01348</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">The natural substrate for this enzyme may be peptidyl-tRNAs which drop off the ribosome during protein synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>an N-acyl-L-alpha-aminoacyl-tRNA + H2O = an N-acyl-L-amino acid + a tRNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:13883"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59874"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:138191"/>
            <dbReference type="EC" id="3.1.1.29"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the PTH2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.29" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96604.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589933.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWC1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00019"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.264"/>
    <dbReference type="OrthoDB" id="6075at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004045">
        <property type="term" value="F:peptidyl-tRNA hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02430">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1490.10:FF:000001">
        <property type="entry name" value="Peptidyl-tRNA hydrolase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1490.10">
        <property type="entry name" value="Bit1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023476">
        <property type="entry name" value="Pep_tRNA_hydro_II_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002833">
        <property type="entry name" value="PTH2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00283">
        <property type="entry name" value="arch_pth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003314">
        <property type="entry name" value="PRK04322.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12649:SF11">
        <property type="entry name" value="PEPTIDYL-TRNA HYDROLASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01981">
        <property type="entry name" value="PTH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102462">
        <property type="entry name" value="Peptidyl-tRNA hydrolase II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013260"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048707"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00050038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96604.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="104" mass="11465" checksum="4E1635B2EB75D7FB" modified="2013-06-26" version="1">MKMAIVIRNDLKMGKGKMVAQGGHAIIEAFLQAQKRKPELVEKWLKEGQKKVVLKVNSEKELIDIYNKAKAHGLICSIIRDAGLTQLDPGTITAVAIGPDEDEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWK2</accession>
    <name>N6UWK2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amino acid-binding ACT protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00666</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589809.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWK2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00902"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.128"/>
    <dbReference type="OrthoDB" id="146838at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04874">
        <property type="entry name" value="ACT_Af1403"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10550">
        <property type="entry name" value="Hypothetical protein af1403, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054480">
        <property type="entry name" value="AHAS_small-like_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040537">
        <property type="entry name" value="DUF5612"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015832">
        <property type="entry name" value="UCP006363_ACT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22629">
        <property type="entry name" value="ACT_AHAS_ss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18462">
        <property type="entry name" value="DUF5612"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006363">
        <property type="entry name" value="UCP006363_ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="4"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="215" mass="23451" checksum="08685956E8A5DA00" modified="2013-06-26" version="1">MEIGLSIEAENKIGVLHKITGIIFELGGNILYTQQFLKDNDVGFIYMEIEIDDVKKLEDELKKCKFIKSFEIHNTLKKIFGKRVIIIGGGAQVAEVARGAISEADRHNIRGERISVDTIPLVGEENLYEAVKAVAKLPRVGVLVLAGSLMGGKITEAVRELKKNGIIVISLKMFGSVPKEADLVVGDPLQAGVLAVMAIAETAKFDINKVRGRIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWL8</accession>
    <name>N6UWL8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">H/ACA RNA-protein complex component Cbf5p</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00796</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWL8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00877"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.156"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000495">
        <property type="term" value="P:box H/ACA sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990481">
        <property type="term" value="P:mRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031118">
        <property type="term" value="P:rRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031120">
        <property type="term" value="P:snRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21148">
        <property type="entry name" value="PUA_Cbf5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.10">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002501">
        <property type="entry name" value="PsdUridine_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004802">
        <property type="entry name" value="tRNA_PsdUridine_synth_B_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032819">
        <property type="entry name" value="TruB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00425">
        <property type="entry name" value="CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003280">
        <property type="entry name" value="PRK04270.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127">
        <property type="entry name" value="CENTROMERE/MICROTUBULE BINDING PROTEIN CBF5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23127:SF0">
        <property type="entry name" value="H_ACA RIBONUCLEOPROTEIN COMPLEX SUBUNIT DKC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16198">
        <property type="entry name" value="TruB_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01509">
        <property type="entry name" value="TruB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="2">
        <location>
            <begin position="137"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="222" mass="25648" checksum="A4917F19EDC9A647" modified="2013-06-26" version="1">MHLHRDASEEDILRVFKEFTGKIYQRPPLKSAVKKRLRVRRIYEIELLDKEGRDVLFRVKCQSGTYIRKLCEDIGEALGTSAHMQELRRIKSGVFDENDAVYLQDLLDAYVFWKEDGDEEELRRIIKPLEYGLRHLKKVVVKDSAVSAICHGADVYVRGISKLSKDINKGDIVVVETLKGEAIGFGKALMSSKEMLTAEKGKAVDIERVFMERNVYPKMWKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWN3</accession>
    <name>N6UWN3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Signal recognition particle 54 kDa protein</fullName>
            <shortName evidence="10">SRP54</shortName>
            <ecNumber evidence="10">3.6.5.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">srp54</name>
        <name evidence="12" type="ORF">J422_00491</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds to the hydrophobic signal sequence of the ribosome-nascent chain (RNC) as it emerges from the ribosomes. The SRP-RNC complex is then targeted to the cytoplasmic membrane where it interacts with the SRP receptor FtsY.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.4"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9 10">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="10">The SRP-RNC complex is targeted to the cytoplasmic membrane.</text>
    </comment>
    <comment type="domain">
        <text evidence="10">Composed of three domains: the N-terminal N domain, which is responsible for interactions with the ribosome, the central G domain, which binds GTP, and the C-terminal M domain, which binds the RNA and the signal sequence of the RNC.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the GTP-binding SRP family. SRP54 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.4" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96749.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589780.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWN3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00050"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.94"/>
    <dbReference type="OrthoDB" id="52849at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17875">
        <property type="entry name" value="SRP54_G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000022">
        <property type="entry name" value="Signal recognition particle 54 kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.30">
        <property type="entry name" value="Signal recognition particle, SRP54 subunit, M-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00306">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036891">
        <property type="entry name" value="Signal_recog_part_SRP54_M_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004125">
        <property type="entry name" value="Signal_recog_particle_SRP54_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022941">
        <property type="entry name" value="SRP54"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 54K PROTEIN SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11564:SF5">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE SUBUNIT SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02978">
        <property type="entry name" value="SRP_SPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47446">
        <property type="entry name" value="Signal peptide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0733">Signal recognition particle</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="11">
        <location>
            <begin position="265"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="107"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="185"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="244"/>
            <end position="247"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005450"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00064051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96749.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="446" mass="50069" checksum="518DD8E5DDE46F06" modified="2013-06-26" version="1">MLEKMSENLTKAINKIKNAMFVDKKLIKEVVKDIQKALIQADVNVRLVLNLSKEIERRTLEEEPPKGLSKKEHIIKIVYEELVKLLGEEAKPLNIDPKKQNIILLLGLQGSGKTTTAAKLARYLQKKGYRPALICADTYRPAAYEQLKQLADKIHVPVYWDNKKPEEIVKEGLEKFKKADVIIIDTAGRHKEEKGLLEEMKRIKAITNPDEIILVIDGTIGQQAKIQAKAFKEAVGDIGSIIVTKLDGSAKGGGALSAVAEIKAPIKLIGVGEKIDDLEEFNPKKFIARLLGLGDIDSLLKKAEEIVNEKTEESIEAIMRGKFTLNELYAQLEAIENMGSMKKLLSMIPGFSAMPKELSHLTEEKIRKYKVIISSMTKEERENPKIIKASRIKRIARGSGTTERDVREVLRYYETTKGAIDKIRKGKVLKIGGPLGQILRQLMYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWV4</accession>
    <name>N6UWV4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transketolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_00205</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transketolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589730.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWV4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01127"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.41"/>
    <dbReference type="OrthoDB" id="25494at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd02012">
        <property type="entry name" value="TPP_TK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049557">
        <property type="entry name" value="Transketolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005474">
        <property type="entry name" value="Transketolase_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47514">
        <property type="entry name" value="TRANSKETOLASE N-TERMINAL SECTION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47514:SF1">
        <property type="entry name" value="TRANSKETOLASE N-TERMINAL SECTION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00456">
        <property type="entry name" value="Transketolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00801">
        <property type="entry name" value="TRANSKETOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transketolase N-terminal" evidence="6">
        <location>
            <begin position="17"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00456"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="280" mass="30893" checksum="42E5D9D1AE0428F2" modified="2013-06-26" version="1">MMAVNKLDKVDIKTLEKIAKRVRYKVVKMIGLAKSGHPGGSLSAVDVMVALYFAIMDYDPNKKDRDRFVLSKGHAAPALYAILSELNIIDEEELWKLRKLEGKLQGHPSMDTPGVEIATGSLGQGFSAAVGMALGYKIDKLDKHIYVLLGDGECQEGIVWEAAMAAAHYKLDNLIAIVDRNFLQIDGHTEDVMALGNLAEKFKAFGWDVFEIDGHNFKEIIETIEKAKSLKNGKPKMIIAYTTKGKGVSFMEGNVGFHGKAPNEEQLKIALEELKEGKND</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UZW1</accession>
    <name>N6UZW1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family 9</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_07152</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000077">
        <property type="protein sequence ID" value="ENN95563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UZW1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01004"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1382"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008713">
        <property type="term" value="F:ADP-heptose-lipopolysaccharide heptosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03789">
        <property type="entry name" value="GT9_LPS_heptosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002201">
        <property type="entry name" value="Glyco_trans_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051199">
        <property type="entry name" value="LPS_LOS_Heptosyltrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30160">
        <property type="entry name" value="TETRAACYLDISACCHARIDE 4'-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01075">
        <property type="entry name" value="Glyco_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="382" mass="44284" checksum="DDC8FFCBA16A9A00" modified="2013-06-26" version="1">MIKRIKKVLKCENMNIISILRSIDRYIGNLIILFLSIFKTRIHKNLVNVNPKSILIIRLWTLGESLLTLPMIKRLKEEGYNISILVTKRSKGVFENIDFVDEIIDLKNFWKILKNFKKYDVVIDTEPYLNISAILGWFLGKYVIGFDGLFRSKIYDFKIKYNDKIHAVYNFCNLLKPLGIEYKPKRLVPLKYNKKDIDKVENLLKKYNLNNKKLIGIHCGTAETAPWRSWKKEKFAKLIEKLVENGFYIILTGSKNDYGINEEILSLIDKKYKNKVLNFAGKTNLGEFAYLLKKFEIFISNDTGPMHLSAAMGTKTIGLFGPNLPERFAPFGEKNIAIYKAKDLDCSPCINVHKGEFKKCKLNGKCMDMIEVKDVLDEIRRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UZX0</accession>
    <name>N6UZX0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ADP-L-glycero-D-manno-heptose-6-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_07132</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000075">
        <property type="protein sequence ID" value="ENN95568.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004595028.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000075.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UZX0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01000"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1379"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008712">
        <property type="term" value="F:ADP-glyceromanno-heptose 6-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011912">
        <property type="entry name" value="Heptose_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02197">
        <property type="entry name" value="heptose_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43103:SF3">
        <property type="entry name" value="ADP-L-GLYCERO-D-MANNO-HEPTOSE-6-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43103">
        <property type="entry name" value="NUCLEOSIDE-DIPHOSPHATE-SUGAR EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1" id="KW-0521">NADP</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="4">
        <location>
            <begin position="3"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95568.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="308" mass="35339" checksum="8D5E16F38A1D37F0" modified="2013-06-26" version="1">MKVLITGGAGFIGSNLALTLQKNYEVVVLDDFSSGHFKNLIGFEGDIISESILDVDLNQFKDVDIIFHQAAITDTTVSDQRLMMKINTEGFKRFLNFAIENNIIFIYASSAATYGNAPAPQKEEDAGKPNNIYGFSKWICDCIAKRYMEKYPDAHIVGLRYFNVFGPREQFKGKMASMVWQLMKQMLEGKRPRIFKWGEQKRDQVYVKDIVNINLLAMKAKESCIVNAGTGRAVSFNHIIEVLNSILGFEYEPEYIDNPYGEFYQNHTEADLTKAKKYLGYKPKWTFEDAVKDYVKWLKKNNYITVLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6V051</accession>
    <name>N6V051_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Precorrin-6x reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_06543</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000054">
        <property type="protein sequence ID" value="ENN95678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593666.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000054.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V051"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01086"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1273"/>
    <dbReference type="OrthoDB" id="6027at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016994">
        <property type="term" value="F:precorrin-6A reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003723">
        <property type="entry name" value="Precorrin-6x_reduct"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00715">
        <property type="entry name" value="precor6x_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36925">
        <property type="entry name" value="COBALT-PRECORRIN-6A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36925:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-6A REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02571">
        <property type="entry name" value="CbiJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51014">
        <property type="entry name" value="COBK_CBIJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="246" mass="28193" checksum="9367A3F5375663D2" modified="2013-06-26" version="1">MGGTRDSKIIGEKLKKYKIIYTATTEYGAMLGKDFAYKIISKPLDKEDLKKVIEDYKVKLLIDATHPFAVNASRNAIDVCKELGIEYIRFERECEKINHENVIYVKNFEEAFNIAKNFNRIFFMAGIKNLKRAVEVLGDKIIARVLPISVMEALKLLPQQNIVAMYGTFSKGLNRELILNYKCDAIITKESGEFGGFKEKVYGAIEANAKVIIVEKPKLDYPKVFYDIDELVKEVNKIYINSYQSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6V089</accession>
    <name>N6V089_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">acetate--CoA ligase (ADP-forming)</fullName>
            <ecNumber evidence="2">6.2.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_06224</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>acetate + ATP + CoA = acetyl-CoA + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:15081"/>
            <dbReference type="ChEBI" id="CHEBI:30089"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.2.1.13"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.13" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593344.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V089"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00293"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1212"/>
    <dbReference type="OrthoDB" id="18103at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043758">
        <property type="term" value="F:acetate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014089">
        <property type="entry name" value="AcCoA-synth-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051538">
        <property type="entry name" value="Acyl-CoA_Synth/Transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003781">
        <property type="entry name" value="CoA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043938">
        <property type="entry name" value="Ligase_CoA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032875">
        <property type="entry name" value="Succ_CoA_lig_flav_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02717">
        <property type="entry name" value="AcCoA-syn-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43334:SF2">
        <property type="entry name" value="ACETATE--COA LIGASE [ADP-FORMING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13549">
        <property type="entry name" value="ATP-grasp_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13380">
        <property type="entry name" value="CoA_binding_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19045">
        <property type="entry name" value="Ligase_CoA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13607">
        <property type="entry name" value="Succ_CoA_lig"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00881">
        <property type="entry name" value="CoA_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CoA-binding" evidence="6">
        <location>
            <begin position="3"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="687" mass="76525" checksum="208F77DE1589ABEA" modified="2013-06-26" version="1">MKLFYPKSVAVIGATKKEGKVGYAILKNLLKFNGKIYPVNPKYDNIFNLKCYKSVLDIDDEIDLAIIAVPNVAVPQVLEECGKKGIKYAVIISAGFSEVGNYELEEEVKNIIKKYGIRVIGPNCLGIMNTHINLNATFSKIFPPKGGVSIVSQSGAVLNAILDIAPLLNIGFSKVVSLGNKIDIQESDLLEYFLEDDDTEIIVLYIEGLKDKRFLKVAKKVAKKKPIIVLKAGRSEEGKRAAKSHTGSLAGDDVVYDTAFKEAGIIRAKTFEELIDLIHIFSTQPIMEKNEIGVITNAGGFGVLAADACADHNLKLANFEKSTIEKLKEVLPETANITNPLDIIGDATPERYEKATKILLEDKNIHGLLVILTPQEMTEPLKVAEILANLKRDKPLITSFVGGVSVKGAKSYLRKHGIPAYITPENGIKALSYLYKYSLIKVKEDYDPYLDKIEKEFLKIYEENKEIIKSLLDRPNEYKAKQLLKLYGFDIPKGYLAKSKYEAIEYAKKFKKVVLKIVSPQILHKTEAHGVIINPDDVGEAYDMLIKNALEYGKRKNINVEIEGVLVEEFIDGDKVELIIGGKRDEIFGPVIMVGLGGVFVEVLKDVAFGIYPITKDFAHEMLKSLKSYKILEGVRGREGKDIDYIIETLMKISVFMNIHKEIKELDLNPIFVFKKGGCIADARIIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0Q9</accession>
    <name>N6V0Q9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thymidylate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05319</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95898.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592377.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0Q9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01323"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1038"/>
    <dbReference type="OrthoDB" id="51323at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003745">
        <property type="entry name" value="DUF166"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02593">
        <property type="entry name" value="DUF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95898.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="240" mass="28234" checksum="8505249F0BC4F61A" modified="2013-06-26" version="1">MKALFLYHEGNVIMERFYKNLINENFCNVCEECDNCRGDWSYKDDVEAIVIRQPEEEFIEDPEDYIPQLPKADVCIVQLHEDLLYELPYILKDLGYRYFIIPSERPNDLSLAMRKKLKEICSKFNISFYNPKPFCSLKEPKEFIEYFRIGYPKFKITIDGNKIKDIKVLISSPCGESYYIAKRLLGKDINEIKEEIANAHHNYPCLASMEIDKELNDTILHKAGYIALESIMKEIKCLKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6V123</accession>
    <name>N6V123_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05105</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95993.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592155.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V123"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00468"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1001"/>
    <dbReference type="OrthoDB" id="18579at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018665">
        <property type="entry name" value="DUF2122_RecB-nuclease-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09895">
        <property type="entry name" value="DUF2122"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95993.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="145" mass="16183" checksum="60EDFAB4150D15D4" modified="2013-06-26" version="1">MFICLHNTYSSKQVEEFGRIAYGFGANAVIITKATASAAQSGIPILHKMAYKLNKNILCFEDIDDAIELLNPEKIFLIGHKGICDEKMNFEEVGERDLIIFCGASTGFTKLELEKGLGRYIVENDIGALGNLAIFLYEMKKLNRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6V135</accession>
    <name>N6V135_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04660</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96003.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V135"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00950"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.911"/>
    <dbReference type="OrthoDB" id="64057at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051667">
        <property type="entry name" value="Archaeal_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096:SF1">
        <property type="entry name" value="AAA+ ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37096">
        <property type="entry name" value="YALI0E33429P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="69"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96003.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="72" mass="8682" checksum="68DB88FE0E3B31E6" modified="2013-06-26" version="1">MKFYNREEEVNYLKTYCQLEPNTILFVYGSKSSGKITVMLKVIEELSEKDIIFFYYDLRKYATSTKEEFLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>N6V140</accession>
    <name>N6V140_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04685</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96008.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591973.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V140"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00955"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.916"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="28"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96008.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="58" mass="6967" checksum="87D82EA31A4509B6" modified="2013-06-26" version="1">MEREKIIKKLLHTLEHTEEHFETIIQLLKDLNLDCKEYEELYNKLKEANEKIKKVVGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1G2</accession>
    <name>N6V1G2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">ATP-grasp domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_04528</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96103.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591840.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1G2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00619"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.888"/>
    <dbReference type="OrthoDB" id="133985at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.36.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003806">
        <property type="entry name" value="ATP-grasp_PylC-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024710">
        <property type="entry name" value="MfnD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040803">
        <property type="entry name" value="MfnD_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053510">
        <property type="entry name" value="Tyramine-Glutamate_Synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040722">
        <property type="entry name" value="MfnD_Meth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02655">
        <property type="entry name" value="ATP-grasp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18301">
        <property type="entry name" value="preATP-grasp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016766">
        <property type="entry name" value="UCP016766_ATPgrasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="3">
        <location>
            <begin position="93"/>
            <end position="267"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96103.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="282" mass="32147" checksum="51AF3949D2699AF7" modified="2013-06-26" version="1">MFFEYALATGLEDEIIKEGKLMFNTLLYQFLKIDNVISFINKDYNDYKNEGLKVIETNDNNLMEKIREVIKNYKIDYFLVIAPEEDGILYKLTEFIERYGIKNLGSSSEAVKVAGDKYKTYLAIKDIVRTPKTIKGKYIIKKIDGCGGYHQLINENYIIQEYIEGESLSVSLIVGKKIYPLSLNRQYIDKKFVGADVNIDHNLKSEIFNEVVKAVKAIKGLNGYVGVDVVVNKNLIYIIEINPRITTCICKLNTYPTLAELLIKNANGEDLNFNVSGGSFRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1G9</accession>
    <name>N6V1G9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Thiamine pyrophosphate protein TPP binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_04103</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the TPP enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000027">
        <property type="protein sequence ID" value="ENN96113.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591614.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1G9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01354"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.803"/>
    <dbReference type="OrthoDB" id="6837at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005948">
        <property type="term" value="C:acetolactate synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012000">
        <property type="entry name" value="Thiamin_PyroP_enz_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045229">
        <property type="entry name" value="TPP_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968:SF13">
        <property type="entry name" value="ACETOLACTATE SYNTHASE CATALYTIC SUBUNIT, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR18968">
        <property type="entry name" value="THIAMINE PYROPHOSPHATE ENZYMES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00205">
        <property type="entry name" value="TPP_enzyme_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0786">Thiamine pyrophosphate</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme central" evidence="4">
        <location>
            <begin position="175"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="5">
        <location>
            <begin position="329"/>
            <end position="465"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="RuleBase" id="RU362132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96113.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="478" mass="54570" checksum="6A5CF037991DFAA6" modified="2013-06-26" version="1">MKFLEAIVDFLERNIKTLFYYPGEQVLPLCEEVENSKINNILAGDERGAGFMAEGYARITNYIGVAISSTPGAGNLVTPALNAYKDNSSILLITGRNYKKYRGENYFQEIDMNFLNIYKGYFDPKYEDFVKAFYECLYSRRPIHINISLDCYDKEVEVRDYIKYKYNYNFNIDIESRKPLLLIGQGIYGLMPYKDIIKINKILKSVQIPISTTFPARGVISENSDNCIGLVGRRGDIKSLLEADKIINIGSSLSYNTYVESVRNELLKKTININFYPNDIKEVKELFNNISLDNLWFEKELYFTPKGDYSKKIYTLISSLPDDAIIVNDAGKHTVFTCLLKKCYLPKSLIASHSFGCMGFSLPTAIGVKLACLDYGIDREVVSINGDGSFIMNLQELKVISENNLKILIVIFENHKLGRFKTINNPNFVKIAESFNIDAYKTENYEEIEDVVKGYLRGNKPLLLSIKTEDEDLPTPNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1H6</accession>
    <name>N6V1H6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Family 2 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04153</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000027">
        <property type="protein sequence ID" value="ENN96123.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591637.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1H6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01344"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.813"/>
    <dbReference type="OrthoDB" id="11098at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04179">
        <property type="entry name" value="DPM_DPG-synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000129">
        <property type="entry name" value="Glycosyltransferase family 2 protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="7"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96123.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="227" mass="25653" checksum="944C894032398F2D" modified="2013-06-26" version="1">MENNVFIVVPAYNEEKMITKTLKALKNEGYKNIIVVDDGSEDNTYELAKREGVIVCRHLINRGLGGALGTGIKCALLYKPKYIITFDADGQHDVKDIKKIIEALKENDFVIGSRLLDKNEIKNMPIIKRIGNFILNLLTFLMGGYYVSDSQSGLRGFTYDAAKKIIKELKSDRYEVSSEFIIIAKQLKLKVKEVPIKTIYTDYSIKKGTNVLTGIKILIKLICQKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1I4</accession>
    <name>N6V1I4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Precorrin-2 C20-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_04078</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000026">
        <property type="protein sequence ID" value="ENN96133.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591537.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1I4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00729"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.799"/>
    <dbReference type="OrthoDB" id="23546at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030788">
        <property type="term" value="F:precorrin-2 C20-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11645">
        <property type="entry name" value="Precorrin_2_C20_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012382">
        <property type="entry name" value="CobI/CbiL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006364">
        <property type="entry name" value="CobI/CbiL/CobIJ_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01467">
        <property type="entry name" value="cobI_cbiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43467">
        <property type="entry name" value="COBALT-PRECORRIN-2 C(20)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43467:SF2">
        <property type="entry name" value="COBALT-PRECORRIN-2 C(20)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036427">
        <property type="entry name" value="Precrrn-2_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="8">
        <location>
            <begin position="5"/>
            <end position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR036427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96133.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="228" mass="26117" checksum="1C39FB2135704398" modified="2013-06-26" version="1">MPLKKVYGVGVGPGDRELITLKALRVLKEVNKVFVPISKLGRRSLAYEIIKDFIKDKHIEELLFPMVKDKNKLKSYWDEALRKILKEDGEVAIVTIGDPTLYSTFSYLWKLLKENGVEVEVINGVSSIFASAKALNMPLVEGDEKLCILPQGKDLYKYIDEFDTIIVMKTKNLKECLKDIMNRDDILIGLVKKASFNDEKVFFGKIKDAKFEEFNDYLSMAIIKKVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1L9</accession>
    <name>N6V1L9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl14</name>
        <name evidence="8" type="ORF">J422_03958</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds to 23S rRNA. Forms part of two intersubunit bridges in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Forms a cluster with proteins L3 and L24e, part of which may contact the 16S rRNA in 2 intersubunit bridges.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uL14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96178.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591402.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1L9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00421"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.776"/>
    <dbReference type="OrthoDB" id="23569at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00337">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.150.20:FF:000007">
        <property type="entry name" value="50S ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.150.20">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01367">
        <property type="entry name" value="Ribosomal_uL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000218">
        <property type="entry name" value="Ribosomal_uL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019971">
        <property type="entry name" value="Ribosomal_uL14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019972">
        <property type="entry name" value="Ribosomal_uL14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036853">
        <property type="entry name" value="Ribosomal_uL14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006344">
        <property type="entry name" value="PRK08571.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03673">
        <property type="entry name" value="uL14_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761">
        <property type="entry name" value="50S/60S RIBOSOMAL PROTEIN L14/L23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11761:SF8">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00238">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01374">
        <property type="entry name" value="Ribosomal_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50193">
        <property type="entry name" value="Ribosomal protein L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00049">
        <property type="entry name" value="RIBOSOMAL_L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96178.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="132" mass="14237" checksum="0B47EAB54C36ADB9" modified="2013-06-26" version="1">MKAIASKPVRGLPVGARCVCADNTGAKEVEIIAVRGYKGVMRRLPSAGVGDMVIVTVKKGTPEMRKQVLPAVIIRQRKEIRRPDGTRVKFFDNAVVIVTPDGNPKGSDIKGPVAKEAAERWPGIARIAKIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1R5</accession>
    <name>N6V1R5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Anthranilate synthase component 1</fullName>
            <ecNumber evidence="11">4.1.3.27</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">trpE</name>
        <name evidence="15" type="ORF">J422_03513</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the anthranilate synthase component I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000022">
        <property type="protein sequence ID" value="ENN96233.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1R5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01331"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.685"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00040"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.120.10">
        <property type="entry name" value="Anthranilate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005801">
        <property type="entry name" value="ADC_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019999">
        <property type="entry name" value="Anth_synth_I-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006805">
        <property type="entry name" value="Anth_synth_I_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010116">
        <property type="entry name" value="Anthranilate_synth_I_arc_typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015890">
        <property type="entry name" value="Chorismate_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01820">
        <property type="entry name" value="TrpE-arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236">
        <property type="entry name" value="AMINOBENZOATE/ANTHRANILATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11236:SF9">
        <property type="entry name" value="ANTHRANILATE SYNTHASE COMPONENT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04715">
        <property type="entry name" value="Anth_synt_I_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00425">
        <property type="entry name" value="Chorismate_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00095">
        <property type="entry name" value="ANTSNTHASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56322">
        <property type="entry name" value="ADC synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Anthranilate synthase component I N-terminal" evidence="14">
        <location>
            <begin position="10"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="Chorismate-utilising enzyme C-terminal" evidence="13">
        <location>
            <begin position="189"/>
            <end position="436"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="149"/>
            <end position="176"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU364045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN96233.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="451" mass="52039" checksum="F4D4402C88F24511" modified="2013-06-26" version="1">MVEVLKMKKTPLEVYNNIKGENSFLLESAEVNKENRYTILGTAEGKITFEKGRLKVESFSELSDYAKDLEGKYNCPLEALREVRNELREHIELPKVLKFKGGLVGYLGYDIVRYWIDLKGNTVNDLKFPDVEFFIVKNFIAFDLKEEKIYLIAEDLEKLNELKKILEEEREVKENTPNIKLNIESNMSKDEFISAVERAKEYIFAGDIFQVVLSRRIKVNIDGLDTFLIYKKVREINPSPYMYYLDFGERKIIGSSPEILVKTNENREVITRPIAGTIRRGKDEKEDKELERILLNDEKERAEHVMLVDLARNDIGKISKYGSVKVTEFMKIEKYSHVQHIVSNVIGKLKDKYDSFLAVKATFPAGTLSGAPKVRAMEIIDELEKSYRGPYGGGVGYFSWDDVMDMAITIRTYVVKKDNAYIQVGAGIVADSIPEKNGKRQRERVWLILRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2N6</accession>
    <name>N6V2N6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9">Argininosuccinate synthase</fullName>
            <ecNumber evidence="3 9">6.3.4.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Citrulline--aspartate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">argG</name>
        <name evidence="12" type="ORF">J422_01855</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>L-citrulline + L-aspartate + ATP = 2-(N(omega)-L-arginino)succinate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10932"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.4.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 9">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the argininosuccinate synthase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.5" evidence="3 9"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96518.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2N6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00219"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.365"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00113"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004055">
        <property type="term" value="F:argininosuccinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000053">
        <property type="term" value="P:argininosuccinate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000050">
        <property type="term" value="P:urea cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01999">
        <property type="entry name" value="ASS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000019">
        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1260.10:FF:000007">
        <property type="entry name" value="Argininosuccinate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1260.10">
        <property type="entry name" value="Argininosuccinate synthetase, chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00005">
        <property type="entry name" value="Arg_succ_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048268">
        <property type="entry name" value="Arginosuc_syn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048267">
        <property type="entry name" value="Arginosuc_syn_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001518">
        <property type="entry name" value="Arginosuc_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018223">
        <property type="entry name" value="Arginosuc_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023434">
        <property type="entry name" value="Arginosuc_synth_type_1_subfam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024074">
        <property type="entry name" value="AS_cat/multimer_dom_body"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00032">
        <property type="entry name" value="argG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001770">
        <property type="entry name" value="PRK00509.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010392">
        <property type="entry name" value="PRK13820.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11587:SF2">
        <property type="entry name" value="ARGININOSUCCINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20979">
        <property type="entry name" value="Arginosuc_syn_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00764">
        <property type="entry name" value="Arginosuc_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69864">
        <property type="entry name" value="Argininosuccinate synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00564">
        <property type="entry name" value="ARGININOSUCCIN_SYN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00565">
        <property type="entry name" value="ARGININOSUCCIN_SYN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginosuccinate synthase-like N-terminal" evidence="10">
        <location>
            <begin position="2"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="Arginosuccinate synthase C-terminal" evidence="11">
        <location>
            <begin position="169"/>
            <end position="383"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="6"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>L-citrulline</name>
            <dbReference type="ChEBI" id="CHEBI:57743"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF20979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96518.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="389" mass="44158" checksum="C6A967C43291EDD6" modified="2013-06-26" version="1">MKAVLAYSGGLDTSCCLKLLEDMGYEVISVCVDVGLPYEELKEVEEKAKSLGVLKHYTVDAKEEFVKDYIFRAIKANAMYEGYPLSTALARPLIAKKVVEIAKKEGAEAIAHGCTGKGNDQFRFETTIRILAPEIKIIAPIRDLNLTREEEIEYAKKKNIPIPKESKKYSIDENLWGRSIEGSDLEDPKNFPSEEVYAWTKNPVEDKEEEVVEIEFKEGVPIAINGEKLNPVELIRKANEIAGKHGVGRIDIIEDRIIGLKSREVYECPGAIMLLTAHKALEQLVLTREEIKFKEIVDNLYSELIYKGLWFDPLKEDLDAFIDKTQERVEGKVWLKLFGGSVRVVGRESRYSLYSKELVSFDEKTDQKEVAGMVKYHGIQAVLYRKVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2S9</accession>
    <name>N6V2S9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02025</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96548.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590183.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2S9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00250"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.397"/>
    <dbReference type="OrthoDB" id="240616at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054648">
        <property type="entry name" value="TudS-rel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045597">
        <property type="entry name" value="TudS_rel_CD3072"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96548.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="165" mass="19113" checksum="38663C22D22E42D3" modified="2013-06-26" version="1">MKGKKIAIVAHCILNQNSVVNGLERAEGAFNDVVELLLKKNYAIIQLPCPEMLYLGIDRVGKTKEEYDTEEYRELCREILKSTIKYLREYSKEGFKFILIGIEGSPTCGIFKTVTKEGLIDGRGILMEEFLKMLENEHIDLKKIDFPVNMNEYNKFLKELERLLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6V300</accession>
    <name>N6V300_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">2-(3-amino-3-carboxypropyl)histidine synthase</fullName>
            <ecNumber evidence="3 10">2.5.1.108</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_01393</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the first step of diphthamide biosynthesis, i.e. the transfer of the 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to the C2 position of the imidazole ring of the target histidine residue in translation elongation factor 2 (EF-2).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>L-histidyl-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36783"/>
            <dbReference type="Rhea" id="RHEA-COMP:9748"/>
            <dbReference type="Rhea" id="RHEA-COMP:9749"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:29979"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73995"/>
            <dbReference type="EC" id="2.5.1.108"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 10">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Protein modification; peptidyl-diphthamide biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the DPH1/DPH2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.108" evidence="3 10"/>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589942.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V300"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00010"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.273"/>
    <dbReference type="OrthoDB" id="314at2157"/>
    <dbReference type="UniPathway" id="UPA00559"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0090560">
        <property type="term" value="F:2-(3-amino-3-carboxypropyl)histidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11840">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11850">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11860">
        <property type="entry name" value="Diphthamide synthesis DPH1/DPH2 domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016435">
        <property type="entry name" value="DPH1/DPH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042263">
        <property type="entry name" value="DPH1/DPH2_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042264">
        <property type="entry name" value="DPH1/DPH2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042265">
        <property type="entry name" value="DPH1/DPH2_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035435">
        <property type="entry name" value="DPH1/DPH2_euk_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022428">
        <property type="entry name" value="Dph2_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03682">
        <property type="entry name" value="arCOG04112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00322">
        <property type="entry name" value="diphth2_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762:SF1">
        <property type="entry name" value="2-(3-AMINO-3-CARBOXYPROPYL)HISTIDINE SYNTHASE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10762">
        <property type="entry name" value="DIPHTHAMIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01866">
        <property type="entry name" value="Diphthamide_syn"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004967">
        <property type="entry name" value="DPH1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00032">
        <property type="entry name" value="Radical_SAM_3-amino-3-carboxyp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 10" id="KW-0408">Iron</keyword>
    <keyword evidence="8 10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR004967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="311" mass="36830" checksum="A7F40FF1D9109164" modified="2013-06-26" version="1">MYDLETERVIEEIKKLGKENPIVIFQAPEGLKLEVEKEIEKIKNKINITPFLWLNSCYGACDLIEDKVKIVNPDLIIHYGHKKLDYVNSEKTIFIPAYYKGDREKIIRDIKEFIKNREFNVITTVQFEKILEEFNPHVIIGCRVEVDSNKDLLFVGTGRFHPLMLAYKYKKDVFIYNPISYKFDKIGREEVEKFIRKRLSAVAKLKFMDIKKVGVVLSVKKGQCRKKKFYEIINLLEKNNINYLSILADEVKPELLFYDVDAYIIIACPRIVLDDYILYKKPIYTPEEFKMFLNSDFNYKLDEVREEDFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6V357</accession>
    <name>N6V357_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Aldehyde ferredoxin oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_00916</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the AOR/FOR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589853.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V357"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00591"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.180"/>
    <dbReference type="OrthoDB" id="84495at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.569.10">
        <property type="entry name" value="Aldehyde Ferredoxin Oxidoreductase Protein, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.9.10">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013984">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013983">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036503">
        <property type="entry name" value="Ald_Fedxn_OxRdtase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001203">
        <property type="entry name" value="OxRdtase_Ald_Fedxn_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036021">
        <property type="entry name" value="Tungsten_al_ferr_oxy-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051919">
        <property type="entry name" value="W-dependent_AOR"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038">
        <property type="entry name" value="ALDEHYDE FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30038:SF7">
        <property type="entry name" value="TUNGSTEN-CONTAINING GLYCERALDEHYDE-3-PHOSPHATE:FERREDOXIN OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01314">
        <property type="entry name" value="AFOR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02730">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00790">
        <property type="entry name" value="AFOR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48310">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, C-terminal domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56228">
        <property type="entry name" value="Aldehyde ferredoxin oxidoreductase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aldehyde ferredoxin oxidoreductase N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00790"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="615" mass="71774" checksum="EF221915DBA3C364" modified="2013-06-26" version="1">MKNALINAKSGKVEIIEKNITPVRWGLYWHKKYETWKYDVYSDKNIFCCGSGIIPLPGGHRLIFSFRSPLWDGFYFSSMGGAGYQFKGSGFNNIAIIGKAEKPSLLVLDNGKVYLEELKENFRTVYEITNYVIENYEDKNLRAVVVGEASKRTNMGALFSQGLKNKKPIKGAEDWAARGGGGSVLYRAHNIAGIVFFGEIEEEDKEKIKKIVEDYYKKPLSKVILEHTSKYRYNKETNTGGTFGNNWLLYKEKVPIYNWRMPYISIEDRKKILEVILKNYLEIFNKEAIETKQWFNCGEPCPVLCKKYRNNNKMDYEPYAANGTLLGIFDLYEADRVVKEVDKLGFDAIEIGNVLAWLFELLDVGLLKEEELEVEKPIFDYNKLFEGDIKEISKHNADQAIKIVNYIAENKNEIYQIIALGKRKASIIFNQKFKDRIKDMKYNDYAAYVPFGSWGEIGPNLYWTPGFFMPLPIQGRYLTYYMPEFHEPEKLAELIYESIKLEMALDNLGVCRFHRRWLKPVLKDICKELLNIDDIEEDSLNLYKEITEFNKKIGYPIKIESERVRDLIIALAKEYNNEKWVKEFENNKEKVDEYIKTVLDKLSELLKIDWSYSLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>N6V371</accession>
    <name>N6V371_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 13 16">Phosphoglycerate kinase</fullName>
            <ecNumber evidence="5 13 16">2.7.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13 17" type="primary">pgk</name>
        <name evidence="17" type="ORF">J422_00616</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 13 16">
            <text>(2R)-3-phosphoglycerate + ATP = (2R)-3-phospho-glyceroyl phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14801"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 13">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 13 16">Belongs to the phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.3" evidence="5 13 16"/>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589803.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V371"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00910"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.118"/>
    <dbReference type="OrthoDB" id="6575at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00185"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043531">
        <property type="term" value="F:ADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004618">
        <property type="term" value="F:phosphoglycerate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000006">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1260:FF:000012">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1260">
        <property type="entry name" value="Phosphoglycerate kinase, N-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00145">
        <property type="entry name" value="Phosphoglyc_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001576">
        <property type="entry name" value="Phosphoglycerate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015824">
        <property type="entry name" value="Phosphoglycerate_kinase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036043">
        <property type="entry name" value="Phosphoglycerate_kinase_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11406:SF23">
        <property type="entry name" value="PHOSPHOGLYCERATE KINASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00162">
        <property type="entry name" value="PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000724">
        <property type="entry name" value="Pgk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00477">
        <property type="entry name" value="PHGLYCKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53748">
        <property type="entry name" value="Phosphoglycerate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 13 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="10 13 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 13 16" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="13 14">
        <location>
            <begin position="19"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 14">
        <location>
            <begin position="57"/>
            <end position="60"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>(2R)-3-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="360"/>
            <end position="363"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00016471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000724-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU000532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN96703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="410" mass="45968" checksum="44760A8995DBD55C" modified="2013-06-26" version="1">MLTLDDFDFDNRSVILRVDINCPINPESGEILDDKRIREVKDTINELQDAKVVIIAHQSRPGKKDFTSLKNHAKRLSEIIGRDVKFVDEVIGSRAKKEIRDMEKGDIIMLENIRFYSEEVLSDWKNWKGITPKKQANTYIIKTLYPMFNYFVNDAFAAAHRAQPSLVGFSYYMPMIAGRLMEREIRVLSKVLNNPEKPCVYVLGGAKADDSIKVIKNVLENGTADKVLTSGIVANIFLAAKGYDLGVNLEIIKNLKFEKLIEVAKELLDKYRDKIVCPIDIALNVNGKREEMELNGKCINYPINDIGEKTIELYREIISEAKTVVANGPAGVFENKDFAKGTIEILKAIANSKAFSVIGGGHLSAAVEMFGLSDKISYISTGGGATLEFLAGEELPVIEMLKESYKRYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3B2</accession>
    <name>N6V3B2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Endonuclease III</fullName>
            <ecNumber evidence="12">4.2.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">DNA-(apurinic or apyrimidinic site) lyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">nth</name>
        <name evidence="15" type="ORF">J422_00486</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>2'-deoxyribonucleotide-(2'-deoxyribose 5'-phosphate)-2'-deoxyribonucleotide-DNA = a 3'-end 2'-deoxyribonucleotide-(2,3-dehydro-2,3-deoxyribose 5'-phosphate)-DNA + a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:66592"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:16897"/>
            <dbReference type="Rhea" id="RHEA-COMP:17067"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:157695"/>
            <dbReference type="ChEBI" id="CHEBI:167181"/>
            <dbReference type="EC" id="4.2.99.18"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Hydrolyzes mismatched double-stranded DNA and polynucleotides, releasing free thymine.</text>
            <dbReference type="EC" id="3.2.2.29"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 12">Belongs to the Nth/MutY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.99.18" evidence="12"/>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96748.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589779.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3B2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00049"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.93"/>
    <dbReference type="OrthoDB" id="84708at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140078">
        <property type="term" value="F:class I DNA-(apurinic or apyrimidinic site) endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141016">
        <property type="term" value="F:G/T mismatch-specific thymine-DNA glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000703">
        <property type="term" value="F:oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006285">
        <property type="term" value="P:base-excision repair, AP site formation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006289">
        <property type="term" value="P:nucleotide-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd10441">
        <property type="entry name" value="GIY-YIG_COG1833"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.340.30:FF:000001">
        <property type="entry name" value="Endonuclease III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00942">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002837">
        <property type="entry name" value="DUF123"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004036">
        <property type="entry name" value="Endonuclease-III-like_CS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000305">
        <property type="entry name" value="GIY-YIG_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000445">
        <property type="entry name" value="HhH_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005759">
        <property type="entry name" value="Nth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43286:SF1">
        <property type="entry name" value="ENDONUCLEASE III-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01986">
        <property type="entry name" value="DUF123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00633">
        <property type="entry name" value="HHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00465">
        <property type="entry name" value="GIYc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01155">
        <property type="entry name" value="ENDONUCLEASE_III_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="15" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="10 12" id="KW-0326">Glycosidase</keyword>
    <keyword evidence="5 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 12" id="KW-0408">Iron</keyword>
    <keyword evidence="7 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0540">Nuclease</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="14">
        <location>
            <begin position="31"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="domain" description="GIY-YIG" evidence="13">
        <location>
            <begin position="243"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="180"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00052915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="SMART" id="SM00465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN96748.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="337" mass="39676" checksum="723B50DCCF748C64" modified="2013-06-26" version="1">MEMIERLLKRIDKKPAVVKDEPFKVLVAALISARTNDNITEKVMKKLFKEVKDLDDLLEIDEERLSSLLYPVGFYRNKAKLLKKLAKVLKERYNGKIPDNLEDLLSLPGVGRKTANLVLTLAFDKDGIAVDTHVHRITNRWEIVDTETPEETEMVLRKKLDRKYWKVINHILVVFGREICGVKPKCSICYKEIKELCPYYKKLLHFEHIIDKFKYKKVSKSKIPNEKGSYILKIKLKKGRLIRFGKNEKYFKSGYYFYVGSAMNGLKNRIERHLKNEKKMFWHIDYLLNYGKIEEIYITNNNVECETANEFIKAFDYIEGFGCSDCSCKSHLFYMKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP18</accession>
    <name>N6VP18_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">5,10-methylenetetrahydromethanopterin reductase</fullName>
            <ecNumber evidence="4">1.5.98.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Methylene-H(4)MPT reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">mer</name>
        <name evidence="6" type="ORF">J422_07072</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible reduction of methylene-H(4)MPT to methyl-H(4)MPT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + oxidized coenzyme F420-(gamma-L-Glu)(n) + H(+) = 5,10-methylenetetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:21144"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.5.98.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 1/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the mer family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.98.2" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000071">
        <property type="protein sequence ID" value="ENN95580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594848.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000071.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP18"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01227"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1368"/>
    <dbReference type="OrthoDB" id="213164at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00697"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018537">
        <property type="term" value="F:coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016705">
        <property type="term" value="F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01097">
        <property type="entry name" value="Tetrahydromethanopterin_reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.30">
        <property type="entry name" value="Luciferase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01091">
        <property type="entry name" value="F420_mer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050564">
        <property type="entry name" value="F420-G6PD/mer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011251">
        <property type="entry name" value="Luciferase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036661">
        <property type="entry name" value="Luciferase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019946">
        <property type="entry name" value="MeH4methanopterin_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03555">
        <property type="entry name" value="F420_mer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002619">
        <property type="entry name" value="PRK02271.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43244">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43244:SF1">
        <property type="entry name" value="5,10-METHYLENETETRAHYDROMETHANOPTERIN REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00296">
        <property type="entry name" value="Bac_luciferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51679">
        <property type="entry name" value="Bacterial luciferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="2 4" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="3 4 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Luciferase-like" evidence="5">
        <location>
            <begin position="12"/>
            <end position="303"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="331" mass="35565" checksum="7D77022F4C473AD0" modified="2013-06-26" version="1">MKFGIEFVPNEPIQKLCYYVKLAEDNGFEFCWITDHYNNRNVYMTLTAIAMNTNKINLGPGVTNPYVRNPAITASAIATLDEISGGRAVLGIGPGDKATFDSLGIEWIKPVTTLKETVEVIRKLLAGERVSFEGKVVKLAGAALNVKPIKKNVPIYIGAQGPKMLETAGAIADGVLINASNPKDFEAAIPLIKKGAEAAGRKLEDVDVAAYACMSVDKKSEKAKQAAVPVVAFIAAGSPEVVLDRHGIDKEKVNKIREALKKGNFPEAFGLVDDNMLEAFSIYGTPDEVVDKCKELVKMGVTQIVAGSPIGPDKEKAIKLIGKQVIPALKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP29</accession>
    <name>N6VP29_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">ABC transporter releated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_07012</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Probably part of an ABC transporter complex. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000067">
        <property type="protein sequence ID" value="ENN95595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594705.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP29"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00575"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1358"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042626">
        <property type="term" value="F:ATPase-coupled transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03225">
        <property type="entry name" value="ABC_cobalt_CbiO_domain1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000224">
        <property type="entry name" value="Energy-coupling factor transporter ATP-binding protein EcfA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015856">
        <property type="entry name" value="ABC_transpr_CbiO/EcfA_su"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050095">
        <property type="entry name" value="ECF_ABC_transporter_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553:SF27">
        <property type="entry name" value="ENERGY-COUPLING FACTOR TRANSPORTER ATP-BINDING PROTEIN ECFA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43553">
        <property type="entry name" value="HEAVY METAL TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="10">
        <location>
            <begin position="4"/>
            <end position="239"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="274" mass="31561" checksum="EFE8F69BC285597D" modified="2013-06-26" version="1">MEIFKLKEVSYSYGKNCVLEDINMEIYKNEILVILGPNGAGKTTLLKIIDGLIFPKKGEIYFKNIKITESLLHSKEFVREFRSKVGFVFQNPDLMLFNPTVWDEVLYTPLQIYDKEKAIKMAEKALKKLKIYHLKDRHPYNLSGGEKKKVAISSILSSDPEVILMDEPTASLDPKSRNELIDIILDLKKEGKTIIIVTHDLSLASLADRCYIINKRILFEGKVKDMFNLPLEEWNLDVPDITKLFLKLREMGYDVDIPINLKEALNIITKLINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP42</accession>
    <name>N6VP42_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06832</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594238.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP42"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01081"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1325"/>
    <dbReference type="OrthoDB" id="65694at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="112" mass="13448" checksum="E75F13A372C86300" modified="2013-06-26" version="1">MEEIEKIINEKVKLSILARFKSLEKYENKLLKDLAEFEMYSAEDLYQRYLISYNKKPNINITLKDDDIVEILENIVKKESEVIKKAEFIVRQGLIHSLLEDKKYLYFLKNKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPG6</accession>
    <name>N6VPG6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidase A24A domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_05958</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593041.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPG6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00567"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1160"/>
    <dbReference type="OrthoDB" id="65749at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009639">
        <property type="entry name" value="Pept_A24A_C_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000045">
        <property type="entry name" value="Prepilin_IV_endopep_pep"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06819">
        <property type="entry name" value="Arc_PepC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01478">
        <property type="entry name" value="Peptidase_A24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="30"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="53"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="101"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="150"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="180"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="230"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="313"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="domain" description="Prepilin type IV endopeptidase peptidase" evidence="2">
        <location>
            <begin position="10"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase A24A-predicted C-terminal archaea" evidence="3">
        <location>
            <begin position="206"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF06819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="39053" checksum="AB6A670B3D7B4440" modified="2013-06-26" version="1">MDILFIVYIINFIILILASLVDIREKIIPHSYVIIMFLINVITGVYYFGFNAIIALISTFILCIILSIGMGGGDVKVFSSLAPIFAYPNSFIFYFPKYMLIIIFLSIILVAIFPIFRIFLRYWKDILFSSLYLVMVIEILYYIIGKFNISFGWIIIWIYIITSIYISRKVPKYKEYTKKIGYLFFIFLLIMFLFDRAYIINNLINMFIYLLEIIFISIFIYAITGAEICFKKHINELKEGDILRDVIIINGDHVEVKNLNIFKRSKLLLNKENKEIIITDGEGLSKEQIERIKSLYNEKKLPDELNVIKTYPFIPFVALSYLLVVLFIYLGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPI3</accession>
    <name>N6VPI3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF499 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05888</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000045">
        <property type="protein sequence ID" value="ENN95800.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592902.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPI3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00846"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1148"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007555">
        <property type="entry name" value="DUF499"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04465">
        <property type="entry name" value="DUF499"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95800.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1128" mass="130563" checksum="7CB8FF7219D246E9" modified="2013-06-26" version="1">MLIKSTFNKEMVCYKRSHLKRGETMQGLKVWKDVLDETLDMQVAPELGDVINKTAHKIYTEPEEFFKRTYFTDSMVQILDRLLNTLSGKERHNIFLIYSLFGGGKTHTILTIYHALNSPDSLVNYEVLKDYPEDKKEKIKNIGLKLKNLQNQNVKILPIYGKGKLGQPSNPLNFSAYEVKTLWGYIGHCLGKYALVENDDKNLTVPTIDTIRELLKGNKVVFLVDEIVHYIDNLYNSGNEDDRRYAKNICKFFDALCTALIGTDSVMILTLPMEKDGEKVEKGYNREIVLSLHRAVERVGGAELYSPLRTEGISGGEIVEILKKRIFEDIDKDFKEKIIEKYREAYSNTEIFGRFEENLSKTYPFHPDFIATLRTIVERGNLQKTRDMVRITRIVVRNLLQEEEFPTLIMPYHINPRNEKLKGILFGKNQIFADYGGAVLDVDLSLEKFKRFSKPELAEIILRYIFLKTYPYDSPTPLEGFPKKDSIARAVYEISFFEKHALQLTDIPNTISEIEKSSSFIYLNKKDGVFWFWRVANVFQMVESKKEELLTLNIVSVHKKLEEFVKKFAVEGKGLKTKKIGNNITFFKKNSIIVSRDPQEFQDDEDYKLQILIRDDVDKSTLYRIIYQYGTGTRTYRNTITVCYADKALNKLLEITARIMACDKVKGDIKAKYKSYGEEVVQIQTNMVRSIEDNAKAEFDEFVASVFKKVAYPKDNDVEIVNATPTSKSILENVYSALVEWGKIPSNLTFEGLEYYLNEVNITFDKPIRFSELRSIIRTNTKLPFITDEQLKEAIKEGVRDLKIGIERDGWVLFKEVYQNMPNYNESGVPLEDIRENDIILPQIKALNKQINQILKEEKDEVVDNKYIKVWYEVCISDSESLPLRELVEETEDGFVVKPEFVDLVINGYIVRKTEEKIIGERNEFSLKLDRHNIEGKPGENVDVKVVITPLKGRGFEVYLEVEEGELDINHGKVPYETIWHIKIPESKKTYNIRAISEDNIIKTDKITLIPRTDIVEVHELSENLKGGKLLEIKGIKSYDDLDMVPENLNAKVRGVFIIENDEYFRADFNNLSLNVVKYIIEEIEGVMEHKAKLCVDIIFTDEIKIDDLIFEKLKCLNKKAVFKIKKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPM7</accession>
    <name>N6VPM7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease P protein component 2</fullName>
            <shortName evidence="6">RNase P component 2</shortName>
            <ecNumber evidence="6">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Pop5</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rnp2</name>
        <name evidence="7" type="ORF">J422_05638</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eukaryotic/archaeal RNase P protein component 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95850.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592696.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPM7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00266"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1100"/>
    <dbReference type="OrthoDB" id="19261at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3250">
        <property type="entry name" value="Ribonuclease P, Pop5 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00755">
        <property type="entry name" value="RNase_P_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002759">
        <property type="entry name" value="Pop5/Rpp14/Rnp2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038085">
        <property type="entry name" value="Rnp2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016434">
        <property type="entry name" value="Rnp2_archaea"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR15441:SF2">
        <property type="entry name" value="RIBONUCLEASE P_MRP PROTEIN SUBUNIT POP5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01900">
        <property type="entry name" value="RNase_P_Rpp14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004952">
        <property type="entry name" value="RNase_P_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160350">
        <property type="entry name" value="Rnp2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95850.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="125" mass="14849" checksum="AFF364CB5F40CFAC" modified="2013-06-26" version="1">MLKTLPPTLREKKRYLAVKVLYDGFIDEGEFIRLLREAVINYYGYWGTSKANPWVVYYSYPYVIVRCQRENVDFVRSSLILVREYKGKNINIICLGVSGTIRKAKIKFLGIKNPKRWFLVKKYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPS2</accession>
    <name>N6VPS2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05304</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95895.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592374.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPS2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01326"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1035"/>
    <dbReference type="OrthoDB" id="59344at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050312">
        <property type="entry name" value="IolE/XylAMocC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110">
        <property type="entry name" value="HYDROXYPYRUVATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110:SF21">
        <property type="entry name" value="XYLOSE ISOMERASE-LIKE TIM BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="1">
        <location>
            <begin position="42"/>
            <end position="232"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95895.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="248" mass="28574" checksum="B377E0EA9F618C30" modified="2013-06-26" version="1">MKIGISNSLFIDNGADLQKTLEFIEKKTKIAELVCDGKINALENKDVLDNYKLKYTLHAPMEDLNISCHREKIRKASIEYIEEILKIIADKIGVIVIHPGEVFCKDDYEKSLKAIIKSIGELNKLQNEYGVKIAIENMPNLDRKMFKRPEKEIINNLNEVGICLDMGHAHLNNAIEEFLNYKEKIVHVHIHDNLGINDDHLPIGFGKIDFEKYKKDLKKLKAIFILEHKYRDIEYIDSSLNSVKSILS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPY9</accession>
    <name>N6VPY9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rps13p</name>
        <name evidence="7" type="synonym">rps13</name>
        <name evidence="10" type="ORF">J422_04940</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Located at the top of the head of the 30S subunit, it contacts several helices of the 16S rRNA. In the 70S ribosome it contacts the 23S rRNA (bridge B1a) and protein L5 of the 50S subunit (bridge B1b), connecting the 2 subunits; these bridges are implicated in subunit movement.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Part of the 30S ribosomal subunit. Forms a loose heterodimer with protein S19. Forms two bridges to the 50S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95960.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592092.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPY9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00502"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.968"/>
    <dbReference type="OrthoDB" id="372127at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.50:FF:000001">
        <property type="entry name" value="30S ribosomal protein S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.910.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.910.10">
        <property type="entry name" value="30s ribosomal protein s13, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01315">
        <property type="entry name" value="Ribosomal_uS13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027437">
        <property type="entry name" value="Rbsml_uS13_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001892">
        <property type="entry name" value="Ribosomal_uS13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010979">
        <property type="entry name" value="Ribosomal_uS13-like_H2TH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019977">
        <property type="entry name" value="Ribosomal_uS13_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018269">
        <property type="entry name" value="Ribosomal_uS13_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003140">
        <property type="entry name" value="PRK04053.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03629">
        <property type="entry name" value="uS13_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S13/40S RIBOSOMAL PROTEIN S18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10871:SF3">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00416">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002134">
        <property type="entry name" value="Ribosomal_S13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46946">
        <property type="entry name" value="S13-like H2TH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00646">
        <property type="entry name" value="RIBOSOMAL_S13_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50159">
        <property type="entry name" value="RIBOSOMAL_S13_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95960.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="151" mass="17408" checksum="E69D7D05C72E03C1" modified="2013-06-26" version="1">MTEQAEFKYLLRISRTDIDGNKKVIMALQDIYGVGEAMARAIIRVTGIDPNKKAGYLTDEEVKKIEDVLSDPAKYGIPSWMFNRRKDYVTGEDKHVIESDLIIVKQEDINRLKRIKCYRGIRHELGLPVRGQRTRSTFRRGPTVGVSRKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ77</accession>
    <name>N6VQ77_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04600</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000029">
        <property type="protein sequence ID" value="ENN96035.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591916.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ77"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.901"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96035.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="194" mass="22647" checksum="503AD36BC3592BFF" modified="2013-06-26" version="1">MNNQNKTENSATIPIRIDKNTLEIMDTLIKHKVFKSRNQLVNKIIKDFYYRLYCETIALELINKAVDELKSKNIKISRKDLKNAITSILPILVLDISMYYDYLDTLTLVKKIKFQTELLSKLGPLLKKYVEENEGNLEEEDLKEIYDMVLKLLTIVLEIKLGELGDENSNKRKVYEIINPKIKNALKKLEKPKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQJ2</accession>
    <name>N6VQJ2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription factor CBF/NF-Y/histone domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_03788</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds and compact DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils. Increases the resistance of DNA to thermal denaturation (in vitro).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96150.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591349.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQJ2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00390"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.742"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22909">
        <property type="entry name" value="HFD_archaea_histone-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050947">
        <property type="entry name" value="Archaeal_histone_HMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050004">
        <property type="entry name" value="HmfB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004823">
        <property type="entry name" value="TAF_TATA-bd_Histone-like_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF043032">
        <property type="entry name" value="archaea_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47828">
        <property type="entry name" value="ARCHAEAL HISTONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47828:SF1">
        <property type="entry name" value="ARCHAEAL HISTONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00803">
        <property type="entry name" value="TAF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TATA box binding protein associated factor (TAF) histone-like fold" evidence="8">
        <location>
            <begin position="5"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96150.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="67" mass="7398" checksum="75C9DD2123B62D79" modified="2013-06-26" version="1">MGELPIAPFERILKKAGAERVSRAAAEYLAEAIEELALEIAKEAVELAKHAKRKTVKVEDIKLALKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQZ6</accession>
    <name>N6VQZ6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">4-vinyl reductase 4VR domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_03159</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96325.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590792.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQZ6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00178"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.620"/>
    <dbReference type="OrthoDB" id="371687at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1380.20">
        <property type="entry name" value="Trafficking protein particle complex subunit 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024096">
        <property type="entry name" value="NO_sig/Golgi_transp_ligand-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004096">
        <property type="entry name" value="V4R"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35090:SF2">
        <property type="entry name" value="ARSR FAMILY TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35090">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02830">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00989">
        <property type="entry name" value="V4R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111126">
        <property type="entry name" value="Ligand-binding domain in the NO signalling and Golgi transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4-vinyl reductase 4VR" evidence="2">
        <location>
            <begin position="160"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="25"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96325.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="227" mass="26301" checksum="2789F89291DE7F2F" modified="2013-06-26" version="1">MANATAIVEMLRIIDNRAKFMGIKLTMIKNLLEKYKDNKELLKEVLKETEGTKLHDLILEACPYLRDLDKEIKAEEARIKISAEEEKPLKLEDYGFVGKVSLLAYIREYMRKYYLRANTKKVFYQIGKDYAILLNINNYDEMKKFIDTEFGEMEIEKPEPLTIIVRDNKECKNCTSPEPVCYVTAGFIAGCLENIVNRKYIVDVVEKKCLAVGDPYCLFVANRTVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR34</accession>
    <name>N6VR34_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">L-threonylcarbamoyladenylate synthase</fullName>
            <ecNumber evidence="3">2.7.7.87</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">L-threonylcarbamoyladenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">J422_02799</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonine + hydrogencarbonate + ATP = L-threonylcarbamoyladenylate + diphosphate + H2O</text>
            <dbReference type="Rhea" id="RHEA:36407"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:73682"/>
            <dbReference type="EC" id="2.7.7.87"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the SUA5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.87" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96370.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590620.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR34"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00110"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.549"/>
    <dbReference type="OrthoDB" id="39992at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003725">
        <property type="term" value="F:double-stranded RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061710">
        <property type="term" value="F:L-threonylcarbamoyladenylate synthase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.10">
        <property type="entry name" value="DHBP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017945">
        <property type="entry name" value="DHBP_synth_RibB-like_a/b_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006070">
        <property type="entry name" value="Sua5-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050156">
        <property type="entry name" value="TC-AMP_synthase_SUA5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00057">
        <property type="entry name" value="L-threonylcarbamoyladenylate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490">
        <property type="entry name" value="SUA5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17490:SF16">
        <property type="entry name" value="THREONYLCARBAMOYL-AMP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01300">
        <property type="entry name" value="Sua5_yciO_yrdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55821">
        <property type="entry name" value="YrdC/RibB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51163">
        <property type="entry name" value="YRDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="YrdC-like" evidence="12">
        <location>
            <begin position="11"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96370.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="208" mass="23613" checksum="3CD5F81C60E65F7F" modified="2013-06-26" version="1">MRIIKLYNMNPNRAKTILEFLKKEILNGKIIICGTDTLYGISANALDENAVKKIYKIKKRDYNKPISICLKDTNEIEKYAYVNDISKKIINNFLPGPLTIILKKKAIIPNIVSKEYIGIRIPDHEIIRELSIVPLTTTSANVSGKKSPTCVEEIDDEIIKRVDYVVDVGKCKYSKPSTVIKIINNKIELIREGVIPFSEICKVAGYER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR39</accession>
    <name>N6VR39_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CTP synthase</fullName>
            <ecNumber evidence="11">6.3.4.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Cytidine 5'-triphosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Cytidine triphosphate synthetase</fullName>
            <shortName evidence="11">CTP synthetase</shortName>
            <shortName evidence="11">CTPS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">UTP--ammonia ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 14" type="primary">pyrG</name>
        <name evidence="14" type="ORF">J422_02834</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen. Regulates intracellular CTP levels through interactions with the four ribonucleotide triphosphates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>UTP + L-glutamine + ATP + H2O = CTP + L-glutamate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26426"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>UTP + NH4(+) + ATP = CTP + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:16597"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="11">Allosterically activated by GTP, when glutamine is the substrate; GTP has no effect on the reaction when ammonia is the substrate. The allosteric effector GTP functions by stabilizing the protein conformation that binds the tetrahedral intermediate(s) formed during glutamine hydrolysis. Inhibited by the product CTP, via allosteric rather than competitive inhibition.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Pyrimidine metabolism; CTP biosynthesis via de novo pathway; CTP from UDP: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homotetramer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="11">CTPSs have evolved a hybrid strategy for distinguishing between UTP and CTP. The overlapping regions of the product feedback inhibitory and substrate sites recognize a common feature in both compounds, the triphosphate moiety. To differentiate isosteric substrate and product pyrimidine rings, an additional pocket far from the expected kinase/ligase catalytic site, specifically recognizes the cytosine and ribose portions of the product inhibitor.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CTP synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.2" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96375.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590630.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR39"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00116"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.556"/>
    <dbReference type="UniPathway" id="UPA00159">
        <property type="reaction ID" value="UER00277"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097268">
        <property type="term" value="C:cytoophidium"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003883">
        <property type="term" value="F:CTP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044210">
        <property type="term" value="P:'de novo' CTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
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        <property type="match status" value="1"/>
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        <property type="entry name" value="CTP synthase"/>
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        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
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        <property type="entry name" value="Class_I_gatase-like"/>
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        <location>
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            <begin position="1"/>
            <end position="264"/>
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            <position position="382"/>
        </location>
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        <location>
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        <location>
            <position position="12"/>
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        <location>
            <position position="12"/>
        </location>
        <ligand>
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        <location>
            <begin position="13"/>
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        <ligand>
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            <position position="53"/>
        </location>
        <ligand>
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        </ligand>
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        <location>
            <position position="70"/>
        </location>
        <ligand>
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        <ligand>
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        </ligand>
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            <begin position="145"/>
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        </location>
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            <position position="405"/>
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        <ligand>
            <name>L-glutamine</name>
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        </ligand>
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        <ligand>
            <name>L-glutamine</name>
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    </evidence>
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        <source>
            <dbReference type="Pfam" id="PF06418"/>
        </source>
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            <dbReference type="EMBL" id="ENN96375.1"/>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="531" mass="59899" checksum="B4438AD029F73406" modified="2013-06-26" version="1">MKYIFITGGVISSLGKGITAASLGRLLKARGFKVNMIKVDPYLQIDAGTMSPYEHGEVFVTEDGGETDLDLGHYERFIDENLTKDNNITAGKIYWNVLIKERRGDYLGKTVQVIPHITDEIKNWIKRLSKGYDITIVEIGGTVGDIESLPFLEAIRQFKKDVGKDNVLYIHVSLLPYIKTAGEVKTKPTQHSVKELRSIGIQPDILICRTEKPINEKIKEKLSLFCDVDKEAVIEARDAKSIYEVPLNLEKEGLGKLVIKKLKLPDRNPDLKDWRAFVDRVINPLNEITIAIVGKYVELKDAYLSIVEALIHAGAKNDTKVNINWLHAERVENDLEILDKERDENRLDGILVPGGFGKRGVEGKINAIKYARENNIPFLGICLGMQCAVIEFARNVCGLNADSTEFNPETEHPVIDLLPEQKNIKEKGGTMRLGSYKAILKEGTLTYKLYGKKEVYERHRHRYEVNPKYHDILEKHGLIISGKSPDGKLAEFIELKHKFFIATQAHPEFKSRPNTPHPLFDGLVKASLGLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR48</accession>
    <name>N6VR48_9EURY</name>
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        <recommendedName>
            <fullName evidence="4">Class III signal peptide-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06932</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
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        <lineage>
            <taxon>Archaea</taxon>
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            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
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            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000065">
        <property type="protein sequence ID" value="ENN95601.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_APMM01000065.1"/>
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    <dbReference type="AlphaFoldDB" id="N6VR48"/>
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    <dbReference type="PATRIC" id="fig|1069083.5.peg.1343"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95601.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="61" mass="6810" checksum="AF1862C18136E315" modified="2013-06-26" version="1">MILSKRAQLSLEFSLLLTVVILSAIIVGYYLITSAIHVKDTNIDLINKTSRIIESYLSKVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR84</accession>
    <name>N6VR84_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06698</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
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            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000057">
        <property type="protein sequence ID" value="ENN95656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_APMM01000057.1"/>
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    <dbReference type="PATRIC" id="fig|1069083.5.peg.1304"/>
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        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
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        <property type="entry name" value="Uncharacterised protein family UPF0058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002753">
        <property type="entry name" value="UPF0058"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036519">
        <property type="entry name" value="UPF0058_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42203">
        <property type="entry name" value="UPF0058 PROTEIN MJ1205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42203:SF2">
        <property type="entry name" value="UPF0058 PROTEIN MJ1205"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01893">
        <property type="entry name" value="UPF0058"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF140371">
        <property type="entry name" value="Vng1086c-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="99" mass="11711" checksum="761848EFEE34CED7" modified="2013-06-26" version="1">MHKEDLMKLHQFFVYVTKELTLNSHLDEKCKELLSIYESLDIKPHHIHRLKDEQKIAILLLASCIAYHLSKNLEGVHKNLAKKLEENAFKHLKELKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRA0</accession>
    <name>N6VRA0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06523</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000053">
        <property type="protein sequence ID" value="ENN95681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593623.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRA0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00998"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1269"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13489">
        <property type="entry name" value="Methyltransf_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="240" mass="28240" checksum="85A7378AD473680D" modified="2013-06-26" version="1">MTFIFQKELSHIKNFLRLYASNRYIYLRKYIKSNKKSKIRLLDVGCGVHSPKVIEALFSTKVEYYGIDKIDIKDSLDKKYSRMRFIKIDLEKELEILDKKLKNNFFDFIMMNHVIEHLENGLEVVSVLCKKLKEGGGFYIEYPGVRSLSLPSMKGTLNFCDDPTHKRIYSIQEIGNVLLKNNFRIIKAGVVRNPLMISLIPFRLIESIFYNYSKASIFWDLFGFAEFIYAIKKNNSYKHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRB9</accession>
    <name>N6VRB9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Putative signal transduction protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_02110</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96445.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590222.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRB9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01171"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.415"/>
    <dbReference type="OrthoDB" id="43333at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17775">
        <property type="entry name" value="CBS_pair_bact_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044065">
        <property type="entry name" value="ACP_MB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51901">
        <property type="entry name" value="ACP_MB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="13"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="78"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="ACP-type MB" evidence="6">
        <location>
            <begin position="153"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="176"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="179"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96445.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="185" mass="20784" checksum="3332021895B98DE7" modified="2013-06-26" version="1">MEIDYNIQVSEVMSFPVITATKDKTVYDIANIMAENDIGAVVIVENNNPIGIITERDILKRVVAKNLRPKDVLVEEVMSKKLVTVSPEASINDAAKLMAKHKIKRLPVVKEGKLVGIITESDIIKVSPEIINLLAEYASIKEKPTLYETEEEYIEGICESCGAQGRVKYYQGKYLCEDCLEEYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRG6</accession>
    <name>N6VRG6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 11">5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase</fullName>
            <ecNumber evidence="2 11">2.5.1.147</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">FO synthase subunit 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cofH</name>
        <name evidence="15" type="ORF">J422_02285</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the radical-mediated synthesis of 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil from 5-amino-6-(D-ribitylamino)uracil and L-tyrosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>5-amino-6-(D-ribitylamino)uracil + L-tyrosine + S-adenosyl-L-methionine = 5-amino-5-(4-hydroxybenzyl)-6-(D-ribitylimino)-5,6-dihydrouracil + 2-iminoacetate + 5'-deoxyadenosine + L-methionine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55200"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:77846"/>
            <dbReference type="ChEBI" id="CHEBI:85936"/>
            <dbReference type="EC" id="2.5.1.147"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11 12">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11 12">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Cofactor biosynthesis; coenzyme F0 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Consists of two subunits, CofG and CofH.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">The FO synthase complex consists of two subunits, CofG and CofH.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the radical SAM superfamily. CofH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.147" evidence="2 11"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96480.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590296.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRG6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01205"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.449"/>
    <dbReference type="OrthoDB" id="8186at2157"/>
    <dbReference type="UniPathway" id="UPA00072"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0141093">
        <property type="term" value="F:5-amino-6-(D-ribitylamino)uracil--L-tyrosine 4-hydroxyphenyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044689">
        <property type="term" value="F:7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000134">
        <property type="entry name" value="7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01612">
        <property type="entry name" value="FO_synth_sub2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045567">
        <property type="entry name" value="CofH/MnqC-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019940">
        <property type="entry name" value="CofH_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034405">
        <property type="entry name" value="F420"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020050">
        <property type="entry name" value="FO_synthase_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00423">
        <property type="entry name" value="CofH family radical SAM protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03551">
        <property type="entry name" value="F420_cofH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005609">
        <property type="entry name" value="PRK07360.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076">
        <property type="entry name" value="FO SYNTHASE (COFH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43076:SF1">
        <property type="entry name" value="LIPOYL SYNTHASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19288">
        <property type="entry name" value="CofH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004762">
        <property type="entry name" value="CHP00423"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00293">
        <property type="entry name" value="((2_3_4_5-tetrahydroxypentyl)a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01388">
        <property type="entry name" value="7_8-didemethyl-8-hydroxy-5-dea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00343">
        <property type="entry name" value="aminofutalosine_synthase_(mqnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00342">
        <property type="entry name" value="cyclic_dehypoxanthine_futalosi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01064">
        <property type="entry name" value="F420__menaquinone_cofactor_bio"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01389">
        <property type="entry name" value="menaquinone_synthsis_involved"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 11 12" id="KW-0408">Iron</keyword>
    <keyword evidence="8 11 12" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11 12" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="14">
        <location>
            <begin position="44"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 12">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>(3R)-3-methyl-D-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:64642"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>(3R)-3-methyl-D-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:64642"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00064449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR004762-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR004762-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN96480.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="358" mass="40976" checksum="EEAC30BFE8D4117C" modified="2013-06-26" version="1">MDLCKFKEREIKKREALELFEEEDFYDILKFADSLRKEIVGDIVTYVVNRNINFTNICVNNCRFCAFRVNERDKRAYFLDIDEIVKKAIEAKKLGCTEVCIQSGLHPKIDTYYQAKILKEIHEALKPYGDIHIHAFSPMEVYFGAENAGLDIKEALKILKESGLNSMPGTAAEILDDEIREIICPNKIKTKDWIRIIKTAHKLEIPTTATMMYGHIEEYKHIVNHIYIIKEIQEETGGFTEFVPLSFIYQNSPLYKEGLARAGASGIEDLKVIAISRIIFKDILKNIQASWVKLGKKMVQVALRCGANDVGGTLIEENISRSAGSEHGVSLSVKEIREMIERVNLIPKQRDTLYNIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRQ1</accession>
    <name>N6VRQ1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Flagellin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_05863</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Flagellin is the subunit protein which polymerizes to form the filaments of archaeal flagella.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 4">Belongs to the archaeal flagellin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592846.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRQ1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01234"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1143"/>
    <dbReference type="OrthoDB" id="102632at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013373">
        <property type="entry name" value="Flagellin/pilin_N_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02537">
        <property type="entry name" value="arch_flag_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006325">
        <property type="entry name" value="PRK08541.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903:SF1">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Flagellin_arch-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0974">Archaeal flagellum</keyword>
    <keyword evidence="6" id="KW-0966">Cell projection</keyword>
    <keyword evidence="6" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU361282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="235" mass="24681" checksum="6A3F7E8D92240542" modified="2013-06-26" version="1">MIDFLKSKKGAIGIGTLIIFIAMVLVAAVAAAVIINTASNLQHKASRIGQESTKQVASGLQVLNVYGYAPNAPNGNVEKLLIIITPNVGDEVDLSQVIVTLSNGKKKVSLVYGGGNYTVYNVTYNGATDLFNGDGGASGSDIQKLWNDLNNSNDKAIFGLIILHDDDSSLSNLDHPTANFGDKVAIAIALDSASVNMSIAPRDKVSGEVIPEYGAPGIIDFKAPSTFTTKVVMLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRR5</accession>
    <name>N6VRR5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Large ribosomal subunit protein uL18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpl18</name>
        <name evidence="8" type="ORF">J422_01510</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">This is one of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 50S ribosomal subunit. Contacts the 5S and 23S rRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589963.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRR5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00430"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.297"/>
    <dbReference type="OrthoDB" id="8644at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008097">
        <property type="term" value="F:5S rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00432">
        <property type="entry name" value="Ribosomal_L18_L5e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.100:FF:000008">
        <property type="entry name" value="50S ribosomal protein L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01337_A">
        <property type="entry name" value="Ribosomal_uL18_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057267">
        <property type="entry name" value="Rbsml_uL18_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005485">
        <property type="entry name" value="Rbsml_uL18_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057268">
        <property type="entry name" value="Ribosomal_L18"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006342">
        <property type="entry name" value="PRK08569.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410:SF12">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23410">
        <property type="entry name" value="RIBOSOMAL PROTEIN L5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17144">
        <property type="entry name" value="Ribosomal_L5e"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00058">
        <property type="entry name" value="RIBOSOMALL5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="195" mass="22310" checksum="625AF31126E0EB22" modified="2013-06-26" version="1">MAHGPTYRVKFRRRREAKTDYRKRLKLLLSRKPRLVARKTLNHCIAQIIEYDEKGDRTVVSAHSKELEKLGYKGHTGNLVSAYLTGYLLGKKALKKGYTEAVLDIGLHRATKGAAVFAILKGALDAGLDVPHGEEILPDESRIRGEHIKNYALILKEEDPEKYKRQFSKYLEKGLEPEKLPEHFEEIKAKIDSLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRS9</accession>
    <name>N6VRS9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DUF106 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_01545</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96565.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589968.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRS9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00436"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.304"/>
    <dbReference type="OrthoDB" id="84619at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002809">
        <property type="entry name" value="EMC3/TMCO1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038978">
        <property type="entry name" value="MJ0935"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42198:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01956">
        <property type="entry name" value="EMC3_TMCO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01415">
        <property type="entry name" value="DUF106"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="28"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="98"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="177"/>
            <end position="195"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="54"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96565.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="198" mass="23452" checksum="4E8F9F5E70C14C5F" modified="2013-06-26" version="1">MFDTIINFFYNSLDAIFLPIIKILHPGMSILFIAFIVSLIITLANKFLIDQERLGEIKREMQEIQKKYKKYMNNPEKLEELKKDQEKMMQLNAEFMKMSFKPMIYTWLPIILIFIYLKHVYGYNGIYQNLYPDWNGVVVYLPTVLAKLLLVDFWHWLGSMLYHGGFKIVSNNALGWLGWYILVSFVTSTVLRKIFGLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRU0</accession>
    <name>N6VRU0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05414</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000039">
        <property type="protein sequence ID" value="ENN95881.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592474.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRU0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00842"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1057"/>
    <dbReference type="OrthoDB" id="64089at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95881.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="126" mass="14451" checksum="830ED3FC67AE0579" modified="2013-06-26" version="1">MELKLPPGLPQSIIARVIEFCNVNYDIREDPALGVKYPVLIGDEESLEKAKEYLKLITELKLALRDIARLARKHKIKAKVYAEDENIRELLKIIKNDIANREFVEVVDEKIEGEVIEVLDKKLIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRU3</accession>
    <name>N6VRU3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit Rpo12</fullName>
            <ecNumber evidence="8">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit P</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 9" type="primary">rpoP</name>
        <name evidence="8" type="synonym">rpo12</name>
        <name evidence="9" type="ORF">J422_01595</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the archaeal Rpo12/eukaryotic RPC10 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589978.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRU3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00446"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.314"/>
    <dbReference type="OrthoDB" id="129238at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.30">
        <property type="entry name" value="RNA polymerase ii, chain L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00615">
        <property type="entry name" value="RNApol_arch_Rpo12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006591">
        <property type="entry name" value="RNAP_P/RPABC4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023464">
        <property type="entry name" value="Rpo12"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001608">
        <property type="entry name" value="PRK00398.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00659">
        <property type="entry name" value="RPOLCX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 8 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 8 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8 9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="46" mass="5501" checksum="0A54B7542A103CB4" modified="2013-06-26" version="1">MVEYKCLNCKKIISYEELGNRARCPYCSYKILIKLRPKVVKHLKAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRV1</accession>
    <name>N6VRV1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ABC transporter periplasmic subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05399</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000038">
        <property type="protein sequence ID" value="ENN95891.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592446.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRV1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00458"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1054"/>
    <dbReference type="OrthoDB" id="24039at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050902">
        <property type="entry name" value="ABC_Transporter_SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535:SF34">
        <property type="entry name" value="MOLYBDATE-BINDING PROTEIN MOLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="5"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95891.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="251" mass="29712" checksum="AFAE678E87CBBB1A" modified="2013-06-26" version="1" fragment="single">KNRYNEGKVYDIGSSTSIDYNIVLNISPDIVFLGDWPQHDAMEEKLKELNLTVSRFYTYIEPTFLGRIEWAKFAAAFWGHNVYEKANNYFQNAWKKYMDLKRKTYGANYINAVTFSAFTSGKVYIPQGQNYYASWINDFRGNYIFSYIPGTKSQKIDTELFVEKAKNADVVIFRQFKNITTADEIMNYYPFLGNIKEWKAYKNGRFYISRPDYYIWEAKDPIGMMEDFAKMLHPEKFPNGDNDLKYYHKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRW8</accession>
    <name>N6VRW8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05359</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95906.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592388.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRW8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01315"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1046"/>
    <dbReference type="OrthoDB" id="64668at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="97"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95906.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="127" mass="15129" checksum="8DC827E365AB3ED4" modified="2013-06-26" version="1">MEIANVIEKLNEIKDDELKDLLKEYIKIKDEISYLNDVLEDVEMLIESIEHIKRDTTAIKAIIPKLSKYTNIPMFNDLIKMIDYVDSVETSEIEALRWKINKDIEELEEKLSMLEKEINIRLREKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRY4</accession>
    <name>N6VRY4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PP-loop domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_01308</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589925.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRY4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00028"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.256"/>
    <dbReference type="OrthoDB" id="33422at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="3">
        <location>
            <begin position="36"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="39"/>
            <end position="41"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="69"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="158"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="278" mass="32148" checksum="18768DC5906AA60F" modified="2013-06-26" version="1" fragment="single">KMFNIPVIYSKTYEYISTKVGRFINKYKILSARDTVLVGFSGGKDSLILLHLLEPYTRKLGIKLIAVTVDVNIGGVRPWKNIDLLKYHCDMLNIPLVVLKSDLDVVELSKILTEHSKGMEFSPCFSCSIIKRYLIGKYAKELAEKENIDYEKIKLAYGHNLDDNSDTILANLFKGEKIRYMRPVTKFKSNVVDFIKFKLDLEPLTIIRPMLQILEDDIVKALDECNLEYYKDKEVCPYSRDRGDSIRRRCHEILEELEKEIPNIREMIVSSAIKTIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS01</accession>
    <name>N6VS01_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Molybdenum cofactor synthesis domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_01136</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MoeA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589895.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS01"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00815"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.222"/>
    <dbReference type="OrthoDB" id="31371at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.340.10:FF:000005">
        <property type="entry name" value="Molybdopterin molybdenumtransferase MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF19">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN MJ0666-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="4">
        <location>
            <begin position="175"/>
            <end position="318"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="388" mass="43340" checksum="6E2EB9698414433F" modified="2013-06-26" version="1">MIKKLTSFKEAEKKVFSKIDSYVKNKIKIVPLIDAINKVSAEEIIANENLPYFNKAAMDGYAVIAEDTYGASYTNPIILELSNDVEHGKAKKVFTGDPIPDNTNAVIMKEFCREENGLLEIYKTVHPNENVAKIGEDVKKGDVIINKHEKITPYHINLLASLAIKEIAIYDLKFGIIATGDELEDLENANLEKLKRTKKIVNSNSYMLYALIKDLGFDGKIYGIVRDDKDMLKEMIKKALKENDFIITTGGTSVGDRDLTVKAIKELGNIILHGVNIRPGKPFGLGEVNNKLIFLLSGYPVASAVQFELFIYRYFLKRKSVVLPLKRSLASELGRVDFVRVKIDSGVEPIRISGSGVITSLVKCDGYILIPENVEGYEKGEMVEVFIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS30</accession>
    <name>N6VS30_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rps4p</name>
        <name evidence="7" type="synonym">rps4</name>
        <name evidence="12" type="ORF">J422_04945</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">One of the primary rRNA binding proteins, it binds directly to 16S rRNA where it nucleates assembly of the body of the 30S subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="7">With S5 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 7">Part of the 30S ribosomal subunit. Contacts protein S5. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 9">Belongs to the universal ribosomal protein uS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95961.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592093.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS30"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00501"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.969"/>
    <dbReference type="OrthoDB" id="10429at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000026">
        <property type="entry name" value="30S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01306_A">
        <property type="entry name" value="Ribosomal_uS4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022801">
        <property type="entry name" value="Ribosomal_uS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022802">
        <property type="entry name" value="Ribosomal_uS4_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018079">
        <property type="entry name" value="Ribosomal_uS4_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005710">
        <property type="entry name" value="Ribosomal_uS4_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001912">
        <property type="entry name" value="Ribosomal_uS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003139">
        <property type="entry name" value="PRK04051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01018">
        <property type="entry name" value="uS4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831">
        <property type="entry name" value="30S 40S RIBOSOMAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11831:SF5">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00163">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01390">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00632">
        <property type="entry name" value="RIBOSOMAL_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS4 N-terminal" evidence="11">
        <location>
            <begin position="5"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="RNA-binding S4" evidence="10">
        <location>
            <begin position="106"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003699"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01390"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95961.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="186" mass="22307" checksum="F77D4B7B1BC25160" modified="2013-06-26" version="1">MGDPRRRFPKKYETPNHPWIKERIEREKELCRKYGLRRKREVWKAETLLRKYRRQARRLIRLLDTPQGQKEMEQLFNVLKRYGILKKENPTLDDVLSLTVEDILERRLQTLVFRKGLARTPKQARQLIVHRHIAVNGRIIDAPSYLVKVDEEDKITYAKNSPFNNKDHPERAKIIGITEESVKEEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS41</accession>
    <name>N6VS41_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DsrE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04995</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95971.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS41"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00490"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.979"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34874">
        <property type="entry name" value="PROTEIN YCHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34874:SF1">
        <property type="entry name" value="PROTEIN YCHN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DsrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95971.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="86" mass="9269" checksum="2F6575BDF466412F" modified="2013-06-26" version="1">MEGAEVNIFLIEDGVYVAKKGQNPSEVPNYLELLKNAIELGAVVKVCGPCCKARGLSDSDLIEGVKLATMHDLVAFVKESDNVITF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS49</accession>
    <name>N6VS49_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2209 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00636</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589805.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS49"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00907"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.122"/>
    <dbReference type="OrthoDB" id="120192at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014514">
        <property type="entry name" value="UCP021940"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09974">
        <property type="entry name" value="DUF2209"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021940">
        <property type="entry name" value="UCP021940"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="128" mass="14941" checksum="8F6EF77A6447149B" modified="2013-06-26" version="1">MVKVLAIDISGRHHENKEFFRVYAGVLLEIEADRIVHVEKIDVLIKKEESQKLRDILKETIELINKIGGEFEYILCEKGEFFNLPKEQVSSILKRHVIYPRTRGEKEAINIAHHVSYSVRKLLLNEGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS74</accession>
    <name>N6VS74_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA polymerase sliding clamp</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">Proliferating cell nuclear antigen homolog</fullName>
            <shortName evidence="4">PCNA</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pcn</name>
        <name evidence="9" type="ORF">J422_00801</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Sliding clamp subunit that acts as a moving platform for DNA processing. Responsible for tethering the catalytic subunit of DNA polymerase and other proteins to DNA during high-speed replication.</text>
    </comment>
    <comment type="function">
        <text evidence="6">Sliding clamp subunit. Responsible for tethering the catalytic subunit of DNA polymerase to DNA during high-speed replication.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotrimer. The subunits circularize to form a toroid; DNA passes through its center. Replication factor C (RFC) is required to load the toroid on the DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the PCNA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589830.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS74"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00875"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.158"/>
    <dbReference type="OrthoDB" id="14749at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030337">
        <property type="term" value="F:DNA polymerase processivity factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006272">
        <property type="term" value="P:leading strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006275">
        <property type="term" value="P:regulation of DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00577">
        <property type="entry name" value="PCNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.70.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00317">
        <property type="entry name" value="DNApol_clamp_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046938">
        <property type="entry name" value="DNA_clamp_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000730">
        <property type="entry name" value="Pr_cel_nuc_antig"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022649">
        <property type="entry name" value="Pr_cel_nuc_antig_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022659">
        <property type="entry name" value="Pr_cel_nuc_antig_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022648">
        <property type="entry name" value="Pr_cel_nuc_antig_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00590">
        <property type="entry name" value="pcna"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002219">
        <property type="entry name" value="PRK01115.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002222">
        <property type="entry name" value="PRK01115.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11352:SF0">
        <property type="entry name" value="PROLIFERATING CELL NUCLEAR ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02747">
        <property type="entry name" value="PCNA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00705">
        <property type="entry name" value="PCNA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00339">
        <property type="entry name" value="PCNACYCLIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55979">
        <property type="entry name" value="DNA clamp"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01251">
        <property type="entry name" value="PCNA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 5" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 4 5" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA N-terminal" evidence="7">
        <location>
            <begin position="3"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="domain" description="Proliferating cell nuclear antigen PCNA C-terminal" evidence="8">
        <location>
            <begin position="123"/>
            <end position="243"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="247" mass="27543" checksum="5EC6436E3D172097" modified="2013-06-26" version="1">MRATLESAKEFKKVVDTVAQILDEICLIANEDGITARAMDPSHVALVSLEIPRLSFEDYEIEGEKEIGIDLEAFKKIMNRAKSKDKLILELDEEKNKLNVIFENSGRRKFSLALLDISASEIKMPEIEYPAVVMLKGDAFKEALKDADLISDYVILKVEDNKFIVETKGELNDFSAEFDEDSSALISIESQGHAKSAFNLEYLLDMVKGIKAGDIVKLYLGTDMPLKLEYSLGGANLTFLLAPRIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSA2</accession>
    <name>N6VSA2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">tRNA-t(6)A37 methylthiotransferase</fullName>
            <ecNumber evidence="11">2.8.4.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">J422_04810</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the methylthiolation of N6-threonylcarbamoyladenosine (t(6)A), leading to the formation of 2-methylthio-N6-threonylcarbamoyladenosine (ms(2)t(6)A) at position 37 in tRNAs that read codons beginning with adenine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-SH + AH2 + 2 S-adenosyl-L-methionine = 2-methylsulfanyl-N(6)-L-threonylcarbamoyladenosine(37) in tRNA + (sulfur carrier)-H + 5'-deoxyadenosine + L-methionine + A + S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:37075"/>
            <dbReference type="Rhea" id="RHEA-COMP:10163"/>
            <dbReference type="Rhea" id="RHEA-COMP:11092"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:74418"/>
            <dbReference type="ChEBI" id="CHEBI:74420"/>
            <dbReference type="EC" id="2.8.4.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="11">Binds 1 or 2 [4Fe-4S] cluster. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the methylthiotransferase family. CDKAL1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.5" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96031.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592019.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSA2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00980"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.941"/>
    <dbReference type="OrthoDB" id="372134at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035598">
        <property type="term" value="F:tRNA (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-methylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.12160:FF:000003">
        <property type="entry name" value="CDK5 regulatory subunit-associated protein 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.80.30.20:FF:000002">
        <property type="entry name" value="threonylcarbamoyladenosine tRNA methylthiotransferase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12160">
        <property type="entry name" value="Methylthiotransferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005839">
        <property type="entry name" value="Methylthiotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013848">
        <property type="entry name" value="Methylthiotransferase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038135">
        <property type="entry name" value="Methylthiotransferase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006466">
        <property type="entry name" value="MiaB-like_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01578">
        <property type="entry name" value="MiaB-like-B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00089">
        <property type="entry name" value="MiaB/RimO family radical SAM methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918">
        <property type="entry name" value="RADICAL SAM PROTEINS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11918:SF45">
        <property type="entry name" value="THREONYLCARBAMOYLADENOSINE TRNA METHYLTHIOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00919">
        <property type="entry name" value="UPF0004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01061">
        <property type="entry name" value="methylthiotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51449">
        <property type="entry name" value="MTTASE_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="8 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 11" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 11" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="MTTase N-terminal" evidence="13">
        <location>
            <begin position="1"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="Radical SAM core" evidence="14">
        <location>
            <begin position="127"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="12">
        <location>
            <begin position="359"/>
            <end position="412"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051661"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU368081"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN96031.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="412" mass="47454" checksum="F05A6D49407BE9C5" modified="2013-06-26" version="1">MKVYVEGYGCVLNQADTDIIRNSIKKEGFEITEDINEADIIIINTCVVRLETENRMIYRINELKKLNKFLVVAGCLPKALKEKIGDLPYIYPREAHRAGEILKEYLHKRKRENKEDHKTLYEKLKYISPKLITPLPICEGCLGNCSYCIVKIARGNLISYPKEKIVKKAEELIKGGTKCLFITAQDTACYGLDIGENLANLLNDICSIEGEFIIRVGMMHPKNLERIVDDLIEVYKNDKIGKFLHLPAQSGDDEILKLMNRGYTVDEFKSLVNEFRKKIKNLCFVTDIIVGFPGESDEHFMNTLEFLKEVKPDYIHGAKYSQRKGTLASKMKQVDTKIRKRRSEILDKLRRELSYINNKKYIGKSLRAIVVDKDKGYTENFKTVKLKCEEVGEFKKVKIKDAKTFCLIGEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSA6</accession>
    <name>N6VSA6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00546</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589791.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSA6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00061"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.105"/>
    <dbReference type="OrthoDB" id="116038at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019214">
        <property type="entry name" value="EhaC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011316">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09882">
        <property type="entry name" value="EhaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036534">
        <property type="entry name" value="EhaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="50"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="79" mass="8942" checksum="D2ADCA40CBABC3F9" modified="2013-06-26" version="1">MEEIIYIIAILMLIRIFLERSRERKLLYLCCLSFCISALIALYVKSPMGGIVAITFFVCSTLSSNAIAYTLAQVKDSER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSJ8</accession>
    <name>N6VSJ8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589751.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSJ8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01099"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.64"/>
    <dbReference type="OrthoDB" id="65511at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="200" mass="22712" checksum="D6C724FA2D0349D4" modified="2013-06-26" version="1">MKKLLAIVILFTLLSLCINKNLDIYKDELTFYYNGHPIDVKLRVPISMALETELVNTSDKEIYYLYHTKKNLYIISNLSVSPKGGGVAILELLTKISWLNNFYPHNIILELNRSNNTVYLEFKLVNGKEGEAKLKINECEAIMNSNNTLVILANISDKKARISKVGNKIIIEGDSLEEMDKAVARFIIDIINYTVIGDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSW7</accession>
    <name>N6VSW7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Thioredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_03014</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glutaredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96296.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590747.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSW7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00150"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.591"/>
    <dbReference type="OrthoDB" id="35385at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015035">
        <property type="term" value="F:protein-disulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045454">
        <property type="term" value="P:cell redox homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02973">
        <property type="entry name" value="TRX_GRX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.30.10">
        <property type="entry name" value="Glutaredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011767">
        <property type="entry name" value="GLR_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004502">
        <property type="entry name" value="Thio_glut"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012336">
        <property type="entry name" value="Thioredoxin-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036249">
        <property type="entry name" value="Thioredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013766">
        <property type="entry name" value="Thioredoxin_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00411">
        <property type="entry name" value="redox_disulf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37170:SF1">
        <property type="entry name" value="GLUTAREDOXIN-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37170">
        <property type="entry name" value="GLUTAREDOXIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13192">
        <property type="entry name" value="Thioredoxin_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52833">
        <property type="entry name" value="Thioredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00195">
        <property type="entry name" value="GLUTAREDOXIN_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51354">
        <property type="entry name" value="GLUTAREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51352">
        <property type="entry name" value="THIOREDOXIN_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Thioredoxin" evidence="3">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51352"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96296.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="83" mass="9317" checksum="4D9B26958DD26EFA" modified="2013-06-26" version="1">MAVKIELFTSPMCPHCPAAKRVVEEVVKEFPEVEVEYINVMENPDRAMKYGIMAVPTIVINGEIEFIGAPTKEALIEAIKKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTJ4</accession>
    <name>N6VTJ4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Pyruvate synthase subunit PorA</fullName>
            <ecNumber evidence="2">1.2.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Pyruvate oxidoreductase alpha chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Pyruvic-ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_01715</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate + CoA = 2 reduced [2Fe-2S]-[ferredoxin] + acetyl-CoA + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12765"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="1.2.7.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.1" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96496.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590101.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTJ4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00194"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.337"/>
    <dbReference type="OrthoDB" id="372068at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.920:FF:000010">
        <property type="entry name" value="Pyruvate ferredoxin oxidoreductase, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000012">
        <property type="entry name" value="Pyruvate:ferredoxin (Flavodoxin) oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053390">
        <property type="entry name" value="Pyruvate_synthase_PorA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040682">
        <property type="entry name" value="PorA_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF0">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="8">
        <location>
            <begin position="14"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="9">
        <location>
            <begin position="262"/>
            <end position="358"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00044787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96496.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="380" mass="42118" checksum="DD812B677F60FA85" modified="2013-06-26" version="1">MKKVITGTSAAAEAVRLADVDVIAAYPITPQTTCVEKLAEFIANGELDAEYIKVESEHSAMAACIGAAATGVRTFTATASQGLALMHELLFIAAGMRLPIVMLVANRALSAPINIWCDHQDSISQRDTGWIQIYAESNQETLDSVIQAYKIAENENVLLPVMVCIDGFILTHTVEPVEIPDEKKVREYLGVYEPKHAYLDPDRPITQGPVGVPECYMETRKQIEKAMENAKKVIKDVNEEYYNIFGRKYGNGLVEAYNLENADTVLVAMGSVCGTIKDVIDKMENVGLLRVRCFRPFPKEDIRKFLENAENIAVIDKNISLGFNKGALGIEISSVLKNKKICNYIAGLGGRDVRIEDIKFIIDHVRKADDDETVWVNCKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTK0</accession>
    <name>N6VTK0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Phosphoesterase RecJ domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01740</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96501.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590106.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTK0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00199"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.342"/>
    <dbReference type="OrthoDB" id="36101at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051673">
        <property type="entry name" value="SSDNA_exonuclease_RecJ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30255:SF2">
        <property type="entry name" value="SINGLE-STRANDED-DNA-SPECIFIC EXONUCLEASE RECJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDH" evidence="1">
        <location>
            <begin position="25"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="334"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96501.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="426" mass="48376" checksum="BC12E2AC56DE24EF" modified="2013-06-26" version="1">MLEKLTEMEKVIEDIKERINEQKYVRIVTHHDPDGLSSGSILAKLLLRENKLFQITVVEHLSKNVIDKLSNKYFYIFADMGSGQIEELLNKDIRGVIIDHHPPAIKETHVKDIIQLNPHIFGIDGAKEATASGMCYLLARAYKYYDLSGLAIVGIIGDMQYSPLLGINKFIVNEAREYRHIKIFEDIIYNVYNIELYKAIAYCTKPYIPDLASEVKAYNFLKSLNIDPEKKELTWDESKKLISALIFKYPKADRLKINRIIINHKVKDAFLLSEMLNSVGRHGLFGVGIGIGLEDDKCIERGYEILWEYKKKLIKEIKETKLRKLKNIYYFEGKKGFIGIIASILAEDKPVIGYYIEGDIAKFSARGNKELIERGLDLNEAMAVAKEFDGSGGGHNIAAGATIPKDKIEEFLKRVDEIIGGQLSKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTM3</accession>
    <name>N6VTM3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Large ribosomal subunit protein eL8</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 11" type="primary">rpl7ae</name>
        <name evidence="11" type="ORF">J422_01870</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Multifunctional RNA-binding protein that recognizes the K-turn motif in ribosomal RNA, the RNA component of RNase P, box H/ACA, box C/D and box C'/D' sRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the 50S ribosomal subunit. Probably part of the RNase P complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eukaryotic ribosomal protein eL8 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96521.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590129.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTM3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00222"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.368"/>
    <dbReference type="OrthoDB" id="25810at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042254">
        <property type="term" value="P:ribosome biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000020">
        <property type="entry name" value="50S ribosomal protein L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00326">
        <property type="entry name" value="Ribosomal_eL8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050257">
        <property type="entry name" value="eL8/uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004037">
        <property type="entry name" value="Ribosomal_eL8-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018492">
        <property type="entry name" value="Ribosomal_eL8/Nhp2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022481">
        <property type="entry name" value="Ribosomal_eL8_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03677">
        <property type="entry name" value="eL8_ribo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23105">
        <property type="entry name" value="RIBOSOMAL PROTEIN L7AE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00881">
        <property type="entry name" value="L7ARS6FAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00884">
        <property type="entry name" value="RIBOSOMALHS6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01082">
        <property type="entry name" value="RIBOSOMAL_L7AE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="7 9 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="6 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 9" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="4 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="10">
        <location>
            <begin position="16"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96521.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="117" mass="12827" checksum="E43B5EC7AF7C3F97" modified="2013-06-26" version="1">MAIYVKFKVPEEIQKEILEAIAKAKKIKKGANEVTKAVERGIAKLVIIAEDVKPEEVVAHLPYLCEEKNIPYAYVASKQDLGKAAGIEVAASSVAIIEEDNPDELKALIEKINALKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTU5</accession>
    <name>N6VTU5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">Adenine deaminase</fullName>
            <shortName evidence="6">Adenase</shortName>
            <shortName evidence="6">Adenine aminase</shortName>
            <ecNumber evidence="2 6">3.5.4.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">ade</name>
        <name evidence="9" type="ORF">J422_01625</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>adenine + H2O + H(+) = hypoxanthine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:23688"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="EC" id="3.5.4.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the metallo-dependent hydrolases superfamily. Adenine deaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.2" evidence="2 6"/>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589984.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTU5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00452"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.320"/>
    <dbReference type="OrthoDB" id="24954at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000034">
        <property type="term" value="F:adenine deaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006146">
        <property type="term" value="P:adenine catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01295">
        <property type="entry name" value="AdeC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01518">
        <property type="entry name" value="Adenine_deamin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006679">
        <property type="entry name" value="Adenine_deam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026912">
        <property type="entry name" value="Adenine_deam_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01178">
        <property type="entry name" value="ade"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113:SF2">
        <property type="entry name" value="ADENINE DEAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11113">
        <property type="entry name" value="N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13382">
        <property type="entry name" value="Adenine_deam_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0464">Manganese</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="7">
        <location>
            <begin position="50"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="domain" description="Adenine deaminase C-terminal" evidence="8">
        <location>
            <begin position="380"/>
            <end position="533"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047720"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF13382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="545" mass="61353" checksum="DF3CCD1CA81DB934" modified="2013-06-26" version="1">MILKNAKIINVYTGEILEGNVYFDEKIKLIDFDNNEIKKDDKIINLKGKYLSPTFIDAHIHIESSHLIPSEFEKLVLKSGVSKVVIDPHEIANVCGKDGILFMLNDAKYLDVYVMLPSCVPATELETNGAKITAKDIEELITLDRVLGLGEVMNYKAVINNEKSIIEKINIAKKYKKLIDGHCPGLRGEELNKYIEKGIMSDHEAFEIDEALEKLRLGLKLMIREGTVSKNIYLLEVAKKVKDKRNIILVSDDVSKDLDCYMVKILRKAIKYVSPIEAIQMVTANPANYLGIDIGIKPGNEASFIIFDDIEKFKINNIIIKGKFLNDILKDLNNSKKRIPKTLLKTVNCKIKKEDFLIEGIDYNKKDGKVRVIKPLKDCLITKEKILNVKKAKELLEANKLNKIFVIERHKNTGNVGKGLIEFLNKGTLASTYAHDSHNIIAIGNDIDDLYLAVSVLKSIGGGFVAVLNGKVYKVPLRVAGIMGDSLKTLEEIEKLNKIIEGWCNFEDPFLSMSFFSLPVIPELKITDKGLVKNMELVDLFINSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTV7</accession>
    <name>N6VTV7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome-activating nucleotidase</fullName>
            <shortName evidence="9">PAN</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Proteasomal ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proteasome regulatory ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proteasome regulatory particle</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pan</name>
        <name evidence="12" type="ORF">J422_01500</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">ATPase which is responsible for recognizing, binding, unfolding and translocation of substrate proteins into the archaeal 20S proteasome core particle. Is essential for opening the gate of the 20S proteasome via an interaction with its C-terminus, thereby allowing substrate entry and access to the site of proteolysis. Thus, the C-termini of the proteasomal ATPase function like a 'key in a lock' to induce gate opening and therefore regulate proteolysis. Unfolding activity requires energy from ATP hydrolysis, whereas ATP binding alone promotes ATPase-20S proteasome association which triggers gate opening, and supports translocation of unfolded substrates.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homohexamer. The hexameric complex has a two-ring architecture resembling a top hat that caps the 20S proteasome core at one or both ends. Upon ATP-binding, the C-terminus of PAN interacts with the alpha-rings of the proteasome core by binding to the intersubunit pockets.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">Consists of three main regions, an N-terminal coiled-coil domain that may assist in substrate recognition, an interdomain involved in PAN hexamerization, and a C-terminal ATPase domain of the AAA type.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9 10">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000010">
        <property type="protein sequence ID" value="ENN96596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589961.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTV7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01034"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.295"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022623">
        <property type="term" value="C:proteasome-activating nucleotidase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043335">
        <property type="term" value="P:protein unfolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14686">
        <property type="entry name" value="bZIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19502">
        <property type="entry name" value="RecA-like_PAN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000033">
        <property type="entry name" value="26S protease regulatory subunit 6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000006">
        <property type="entry name" value="26S protease regulatory subunit 8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00553">
        <property type="entry name" value="PAN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050221">
        <property type="entry name" value="26S_Proteasome_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023501">
        <property type="entry name" value="Nucleotidase_PAN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032501">
        <property type="entry name" value="Prot_ATP_ID_OB_2nd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003069">
        <property type="entry name" value="PRK03992.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01242">
        <property type="entry name" value="proteasome-activating nucleotidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23073">
        <property type="entry name" value="26S PROTEASOME REGULATORY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16450">
        <property type="entry name" value="Prot_ATP_ID_OB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7 9" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 9 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9 12" id="KW-0647">Proteasome</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="191"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="9">
        <location>
            <begin position="30"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="202"/>
            <end position="207"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="341"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="416" mass="46972" checksum="DE0669C38ADEE868" modified="2013-06-26" version="1">MVFEEFITGLKKEDKKFTDNLKKDNILENEIKEKSKIAELESKILKLEMEKKELERENLQLLKEIEVLRRELDRMRVPPLIVGTVVDKISDRKVVVKSSTGPNFLVSVSHFVDPDKLTPGTRVCLNQQTLTVVDILPESKDYRAKAMEVDERPNVRYSDIGGLDKQIQEIREVVELPLKHPELFEKVGIEPPKGVLLYGPPGTGKTLLAKAVATETNATFIRVVGSELVKKFIGEGAQLVKDIFKLAKEKAPSIIFIDEIDAIAAKRTEALTGGDREVQRTLMQLLAEMDGFDPRGDVKVIAATNRLDILDPAILRPGRFDRIIEVPPPDEKGRLEILKIHTRNMNLKDVDLEEIAKITEGCVGADIKAICTEAGMNAIREFRDYVTMDDFRKAVEKVMGKRIKKEEPAHLSVLYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU32</accession>
    <name>N6VU32_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF366 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00966</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96696.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589861.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU32"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00582"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.190"/>
    <dbReference type="OrthoDB" id="70011at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007162">
        <property type="entry name" value="DUF366"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04017">
        <property type="entry name" value="DUF366"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006503">
        <property type="entry name" value="UCP006503"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96696.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="193" mass="22649" checksum="6E8D9ECE2A16A98F" modified="2013-06-26" version="1">MKIIKTDYMDIIFAENMEYTGKEIEELWAFKLFNTQKDNIVAFKGRMEVRREFMKDLKDLKRENLDIPIKSELAINFIVEHFDISLREIYLRQRILIFIAKEVIESYGIKLKRDGDDLYYKDRKLSVSIASKGKISGKIHLGINILSKGAEHVKIIGLNDLKIDADEVMEKIAINYAKEIDKIEKDMRKTFPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU53</accession>
    <name>N6VU53_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Methyl-viologen-reducing hydrogenase delta subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_00771</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the MvhD/VhuD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU53"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00882"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.151"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003813">
        <property type="entry name" value="MvhD/FlpD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045873">
        <property type="entry name" value="F420_non_VhuD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02662">
        <property type="entry name" value="FlpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="F420-non-reducing hydrogenase iron-sulfur subunit D" evidence="10">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00034078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="111" mass="12290" checksum="C30D2D686AA04387" modified="2013-06-26" version="1">MQYPPTVRIVRIPCTGTFDITYALRAFQKGADAVFVAGCKVGECAYESGNLRAMERVKFAKMILDELGIGGERLEMFLMSAAEADKFVAAVNEMTERLKKLGPNPLKVKQQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU84</accession>
    <name>N6VU84_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Cupin type-2 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00526</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589787.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU84"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00057"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.101"/>
    <dbReference type="OrthoDB" id="190812at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02214">
        <property type="entry name" value="cupin_MJ1618"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.10">
        <property type="entry name" value="Jelly Rolls"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013096">
        <property type="entry name" value="Cupin_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052044">
        <property type="entry name" value="PKS_Associated_Protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014710">
        <property type="entry name" value="RmlC-like_jellyroll"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011051">
        <property type="entry name" value="RmlC_Cupin_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36114">
        <property type="entry name" value="16.7 KDA PROTEIN IN WHIE LOCUS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36114:SF4">
        <property type="entry name" value="CUPIN 2 CONSERVED BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07883">
        <property type="entry name" value="Cupin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51182">
        <property type="entry name" value="RmlC-like cupins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cupin type-2" evidence="1">
        <location>
            <begin position="36"/>
            <end position="104"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="118" mass="13306" checksum="599D17D8ECEF7B5F" modified="2013-06-26" version="1">MKVNVKDVKAFITKDGSIIRELIHPKFANSKQSLALAEVPINGKTLLHRHKTSEEIYFILEGKGKMILDNKEFEIEMGDTILIPPNTKHKVINTGDKPLKILCASYPAYSDNDTELLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU93</accession>
    <name>N6VU93_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00576</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589797.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU93"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00067"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.111"/>
    <dbReference type="OrthoDB" id="63751at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="66" mass="7388" checksum="E0EBE6FD26E0F9B4" modified="2013-06-26" version="1">MDTERKYGLCLLIIGILSAFGMVITSGWLCYILYSISLPSILYGVGSFLIPKTRRKYPGNLPFRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VUB3</accession>
    <name>N6VUB3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Probable branched-chain amino acid transport ATP-binding protein LivG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_00451</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Probable component of a branched-chain amino-acid transport system.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96786.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589772.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VUB3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00690"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.87"/>
    <dbReference type="OrthoDB" id="44250at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03219">
        <property type="entry name" value="ABC_Mj1267_LivG_branched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000421">
        <property type="entry name" value="Branched-chain amino acid ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051120">
        <property type="entry name" value="ABC_AA/LPS_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772:SF5">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVG-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45772">
        <property type="entry name" value="CONSERVED COMPONENT OF ABC TRANSPORTER FOR NATURAL AMINO ACIDS-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="8">
        <location>
            <begin position="4"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00072811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96786.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="252" mass="28481" checksum="58782D5A636AF6C9" modified="2013-06-26" version="1">MEILKTENVTKYFGDFKALNNVSISVNKGDLTLIIGPNGSGKSTLINVITGFLKADSGKVYFEENDITNKEPEELYYYGIVRTFQTPQPLKEMTVLENILIGNINPGEEPINAIFYSKWLKFEEEMVERAFKILEFLKLSHLYDRKAGSLSGGQMKLLEIGRALMTEPKLIVMDEPIAGVAPGLAHDIFNHVIELKKKGITFLIVEHRLDIVLQYIDHLYVMFNGEIIAEGRGKEIENVINDPKVVEIYIGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VWY2</accession>
    <name>N6VWY2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Oligosaccharide repeat unit polymerase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06962</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000065">
        <property type="protein sequence ID" value="ENN95607.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594639.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VWY2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00859"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1349"/>
    <dbReference type="OrthoDB" id="82276at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002760">
        <property type="entry name" value="O_anti_polymase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01901">
        <property type="entry name" value="O_anti_polymase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="64"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="96"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="222"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="262"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="291"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="314"/>
            <end position="335"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95607.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="359" mass="40672" checksum="BC29609B91C5EF96" modified="2013-06-26" version="1">MIELHHLFIFLIAIYIIFSDVSVNAAIIFLISGIVFYISFIVGKDLFKLDVGKFVGSNYIDRGFGYLLLIIGFVSVVIDLILSGSIPLFDPLSRRFLNVYLTTLSHLFIVGYAIILATENIDKKRAIIYTIIFSIFISLLGYRTNVLALLLSSFAVLYYKGELKKRELLIFGIIIFSILLTLSILRLMFLGALGNPILSRISLTMSIYDIIYNHYNDMFYGFLHYAAIMSYFGHSVGPRYFIAKTLNIEGVTITPTVVGAIIADYGVLAIIPYFGFLGIFLGFLYKLAKRLRGIYLGIFGVLFAYTLISVESGILDLDVIIYYIFGILLCLRTILKSLRNSCYKKYLVILWNSLTEKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX06</accession>
    <name>N6VX06_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase AAA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06753</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000059">
        <property type="protein sequence ID" value="ENN95637.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594056.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX06"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00985"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301:SF8">
        <property type="entry name" value="ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301">
        <property type="entry name" value="DNA-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95637.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="82" mass="10051" checksum="1DD02FD7761790E0" modified="2013-06-26" version="1" fragment="single">MEFFNREKEINEILSIIEFEPNFIYFVYGPINSGKTALINEIINNRLDKDKYVVFYFDLREIFISKYDDFIEVLFEEYNENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX09</accession>
    <name>N6VX09_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glycerophosphoryl diester phosphodiesterase membrane domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06733</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000058">
        <property type="protein sequence ID" value="ENN95642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593987.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX09"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01359"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1310"/>
    <dbReference type="OrthoDB" id="60637at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025098">
        <property type="entry name" value="DUF4013"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13197">
        <property type="entry name" value="DUF4013"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="139"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="227"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="266" mass="30662" checksum="B174B2DAD6E6FFEB" modified="2013-06-26" version="1">MIGDYIKDSYNYIKNNFSKFLIGGILSLISGIFLGVMTFFISYLGNLYDISRVVFLIPIFIIMFIILLTIIFIISGYYVRVMKNTVENKEAPDWDNIWELFVSGLLYTVGMLILSIIFLIIPFIIILMAILFININTKLSVLTIILGLLLFIPFILCLTLYQPLASVNFSVNGFFGFFEFRKIFKLMSLKYVLLLIVVFIISFIVNLIISIPFIILKIVFLIANSDISFMIADILESTVSLFVSFFLGIFSYRCYSLYYKDKIGER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX58</accession>
    <name>N6VX58_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Glutamate N-acetyltransferase</fullName>
            <ecNumber evidence="5">2.3.1.35</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Ornithine acetyltransferase</fullName>
            <shortName evidence="5">OATase</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Ornithine transacetylase</fullName>
        </alternativeName>
        <component>
            <recommendedName>
                <fullName evidence="5">Glutamate N-acetyltransferase alpha chain</fullName>
            </recommendedName>
        </component>
        <component>
            <recommendedName>
                <fullName evidence="5">Glutamate N-acetyltransferase beta chain</fullName>
            </recommendedName>
        </component>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">argJ</name>
        <name evidence="6" type="ORF">J422_06401</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of the acetyl group from N(2)-acetylornithine to glutamate, forming N-acetylglutamate and L-ornithine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(2)-acetyl-L-ornithine + L-glutamate = N-acetyl-L-glutamate + L-ornithine</text>
            <dbReference type="Rhea" id="RHEA:15349"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.3.1.35"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Heterotetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the ArgJ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.35" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593527.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX58"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01294"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1246"/>
    <dbReference type="OrthoDB" id="52592at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00106"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004042">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004358">
        <property type="term" value="F:L-glutamate N-acetyltransferase activity, acting on acetyl-L-ornithine as donor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006592">
        <property type="term" value="P:ornithine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02152">
        <property type="entry name" value="OAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.340">
        <property type="entry name" value="ArgJ beta chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.70.12">
        <property type="entry name" value="L-amino peptidase D-ALA esterase/amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01106">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002813">
        <property type="entry name" value="Arg_biosynth_ArgJ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016117">
        <property type="entry name" value="ArgJ-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042195">
        <property type="entry name" value="ArgJ_beta_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00120">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003802">
        <property type="entry name" value="PRK05388.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23100:SF0">
        <property type="entry name" value="ARGININE BIOSYNTHESIS BIFUNCTIONAL PROTEIN ARGJ, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01960">
        <property type="entry name" value="ArgJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56266">
        <property type="entry name" value="DmpA/ArgJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="3 5" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5 6" id="KW-0808">Transferase</keyword>
    <feature type="chain" id="PRO_5044353283" description="Glutamate N-acetyltransferase alpha chain" evidence="5">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5044353282" description="Glutamate N-acetyltransferase beta chain" evidence="5">
        <location>
            <begin position="175"/>
            <end position="388"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="5">
        <location>
            <position position="175"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="175"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="383"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="388"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="5">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="site" description="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" evidence="5">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <feature type="site" description="Cleavage; by autolysis" evidence="5">
        <location>
            <begin position="174"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="388" mass="42818" checksum="AC0FA14FC7DC4431" modified="2013-06-26" version="1">MEVIKELPKGFKANGYKDGYGFSIIISEKKAIASGVFTRNKVKAYPVIYSKDLLEKKKEFKAIIANSGNAICLIKDGFETVNRIIEKASELFNLNKEDILIASTGVIGRKLDFGMLEERMIKVKELLENGNSIVNAAKGIMTTDKFPKYVAVKIGDVRVCGIAKGAGMINPNMATMLCFILTDIKIDKEDLDKLLKDIVDKTFNNISVDGDTSTNDTVFILANGESGVDYKNCKEDFYKALLFVCRELAKMIVKDGEGATKFLELTIKNAKSREDAKKAGKAIINSLLVKTALFGGDPNWGRIIAALGYSGAEFKDVDLILSNGNEEIYLMRNGIGEDSGDILKKAENIMKGKEIKIIVDLKVGDVDETFYGCDLSYEYVKINAEYTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXA2</accession>
    <name>N6VXA2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cobalamin (Vitamin B12) biosynthesis CbiG protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06085</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593235.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXA2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00536"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1185"/>
    <dbReference type="OrthoDB" id="4722at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.180">
        <property type="entry name" value="CobE/GbiG C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052553">
        <property type="entry name" value="CbiG_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021744">
        <property type="entry name" value="CbiG_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002750">
        <property type="entry name" value="CobE/GbiG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036518">
        <property type="entry name" value="CobE/GbiG_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038029">
        <property type="entry name" value="GbiG_N_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37477:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-5A HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01890">
        <property type="entry name" value="CbiG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11760">
        <property type="entry name" value="CbiG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159672">
        <property type="entry name" value="CbiG N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159664">
        <property type="entry name" value="CobE/GbiG C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cobalamin synthesis G N-terminal" evidence="2">
        <location>
            <begin position="44"/>
            <end position="115"/>
        </location>
    </feature>
    <feature type="domain" description="CobE/GbiG C-terminal" evidence="1">
        <location>
            <begin position="189"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF11760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="322" mass="37595" checksum="203D7467D7A2B81B" modified="2013-06-26" version="1">MIKIIYITESGKKLAEKIKCILDHYKYNSKIFHIKNFSIDEGDEGYIFISAIGIVLRKYIDKIKNDKFTDSFVIVCNEKKEFIPILSNHLGRGNYFSKLIANNINGKTIFTTATDVHGKVGLDELSNILFLEKPNRKDILEINKRLLEENIDLKLPYGWKIKLNGYDVEYHDKDLVIIDNRIKLRPLKIVVGIGARRGVKRYKVYWAIKKALYLRSLYPWRISAFSTIENKRDEIGILEVVNKFKKPFLIFDKDEINKLYNIYDLEKSDFIFKNINVYGVAEPCSILGVKKLTNVDYNKIYLMLKKIKYDGVTIAISTHKEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXE9</accession>
    <name>N6VXE9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">2-phosphosulfolactate phosphatase</fullName>
            <ecNumber evidence="3">3.1.3.71</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_05668</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2R)-O-phospho-3-sulfolactate + H2O = (2R)-3-sulfolactate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:23416"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15597"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58738"/>
            <dbReference type="EC" id="3.1.3.71"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ComB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.71" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592782.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXE9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01269"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1105"/>
    <dbReference type="OrthoDB" id="146693at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050532">
        <property type="term" value="F:2-phosphosulfolactate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050545">
        <property type="term" value="F:sulfopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1560.10">
        <property type="entry name" value="ComB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005238">
        <property type="entry name" value="ComB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036702">
        <property type="entry name" value="ComB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37311">
        <property type="entry name" value="2-PHOSPHOSULFOLACTATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37311:SF1">
        <property type="entry name" value="2-PHOSPHOSULFOLACTATE PHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04029">
        <property type="entry name" value="2-ph_phosp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142823">
        <property type="entry name" value="ComB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00033711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="211" mass="23795" checksum="360FA14064A2E2CF" modified="2013-06-26" version="1">MFDHIYNGFLNQKIKDKVIVVDVLRASTTITTLLSIVDEVYITDSIERREKGIYIGERGGVKVKGFDFNNSPIEILKNRDIIEERYNNGEKIYLSTTNGTRVLRSLKAKEIFIGAIVNAKAVSEVGDATLIPCHRMGEFAIEDIVGCAYIAKYWGNDFGLANPELVINSEAAKHLKAIGYEGDVYFSIMENTYDIVGYYIKEDGKVVRYED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXS4</accession>
    <name>N6VXS4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Class III signal peptide-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04850</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95942.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592055.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXS4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00520"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.950"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007166">
        <property type="entry name" value="Class3_signal_pept_motif"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04021">
        <property type="entry name" value="Class_IIIsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95942.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="74" mass="8093" checksum="7F10CDA7D287050E" modified="2013-06-26" version="1">MMNIIKKIMSNRAQLSMEVGILIAAAVLVAIIAGYLYINNVKSGAEKAGKKTSEFINTTSNKSEDYISKLKNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VY10</accession>
    <name>N6VY10_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">3-octaprenyl-4-hydroxybenzoate carboxy-lyase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_04730</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96017.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VY10"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00965"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.925"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004659">
        <property type="term" value="F:prenyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004507">
        <property type="entry name" value="UbiX-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00421">
        <property type="entry name" value="ubiX_pad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="6" id="KW-0456">Lyase</keyword>
    <keyword evidence="1" id="KW-0637">Prenyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Flavoprotein" evidence="5">
        <location>
            <begin position="1"/>
            <end position="146"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96017.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="157" mass="17578" checksum="1D2CD5D7621B4C05" modified="2013-06-26" version="1">MKIIVGITGASGVIYAKRLLEALDKHETILVISEAGKRVIEEELNMSWEKLKDLANEYYDNNDFFSPYASGSNKFDSAIIIPCSMKTLSAIANGYASSLIVRVADIALKERRKLIICPREMPLNSIHLRNMLKLSNLGAIIMLPYQPFTASQKQLMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>N6VY23</accession>
    <name>N6VY23_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 14">Phosphoribosylamine--glycine ligase</fullName>
            <ecNumber evidence="4 14">6.3.4.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">GARS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 14">Glycinamide ribonucleotide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 14">Phosphoribosylglycinamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">purD</name>
        <name evidence="17" type="ORF">J422_04790</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>5-phospho-beta-D-ribosylamine + glycine + ATP = N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17453"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:58681"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11 14">Belongs to the GARS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.13" evidence="4 14"/>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96027.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592010.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VY23"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00976"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.937"/>
    <dbReference type="OrthoDB" id="146558at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00125"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009113">
        <property type="term" value="P:purine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.600.10">
        <property type="entry name" value="Phosphoribosylglycinamide synthetase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00138">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020561">
        <property type="entry name" value="PRibGlycinamid_synth_ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000115">
        <property type="entry name" value="PRibGlycinamide_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020560">
        <property type="entry name" value="PRibGlycinamide_synth_C-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037123">
        <property type="entry name" value="PRibGlycinamide_synth_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020559">
        <property type="entry name" value="PRibGlycinamide_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020562">
        <property type="entry name" value="PRibGlycinamide_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00877">
        <property type="entry name" value="purD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43472:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLAMINE--GLYCINE LIGASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01071">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02843">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02844">
        <property type="entry name" value="GARS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01210">
        <property type="entry name" value="GARS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00184">
        <property type="entry name" value="GARS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 14 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="10" id="KW-0464">Manganese</keyword>
    <keyword evidence="6 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 14" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="16">
        <location>
            <begin position="108"/>
            <end position="323"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00038345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00042242"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00042864"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00138"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN96027.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="444" mass="48929" checksum="246BB0BADBCF289C" modified="2013-06-26" version="1">MKVLLIGGGARESAIAHALKRGDVELYTLMKNKNPGIAKLSEEIKIAKETDLNAIEEFAKKTNPDLAIIGPEAPLEKGAVDLLESLSIPTVGPSKEAAKIETDKSFMRNLMKKYGIKGSLLYKTFDSYGEDLISFIDELTEKGIKAVVKPVGLTGGKGVKVVGEQLKDNEEAKKYAKEIFEKSIGGGRVIIEEKLEGVEFTLHGFVDSDNIAFTPFVQDHPHALEGDKGSITGGMGSYSCPDHKLPFLTDEDVEEAKEIMSETVKALKEEVGGYKGILYGQFMLTKEGPKIIEYNARFGDPEAMNILAILKNNFIDVCEGIVNRSLNKMNVEFENKATVCKYIAPKGYPDDPVKGFIEVDEGKIKEVGAILHYASVDERDGKLYMTGSRALAVVGVADTIEEAERIAEEATKYVRGEVYHRSDVGKKELIKKRIEKMRKLRQLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYD8</accession>
    <name>N6VYD8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">GMP synthase [glutamine-hydrolyzing] subunit A</fullName>
            <ecNumber evidence="8">6.3.5.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Glutamine amidotransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">guaAA</name>
        <name evidence="10" type="ORF">J422_04073</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 8">Catalyzes the synthesis of GMP from XMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>XMP + L-glutamine + ATP + H2O = GMP + L-glutamate + AMP + diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11680"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.5.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; GMP biosynthesis; GMP from XMP (L-Gln route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer composed of a glutamine amidotransferase subunit (A) and a GMP-binding subunit (B).</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.2" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000026">
        <property type="protein sequence ID" value="ENN96132.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591535.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYD8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00728"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.798"/>
    <dbReference type="OrthoDB" id="10772at2157"/>
    <dbReference type="UniPathway" id="UPA00189">
        <property type="reaction ID" value="UER00296"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003921">
        <property type="term" value="F:GMP synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01742">
        <property type="entry name" value="GATase1_GMP_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000047">
        <property type="entry name" value="GMP synthase [glutamine-hydrolyzing] subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01510">
        <property type="entry name" value="GMP_synthase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004739">
        <property type="entry name" value="GMP_synth_GATase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023686">
        <property type="entry name" value="GMP_synthase_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00888">
        <property type="entry name" value="guaA_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001975">
        <property type="entry name" value="PRK00758.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922:SF2">
        <property type="entry name" value="GMP SYNTHASE [GLUTAMINE-HYDROLYZING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11922">
        <property type="entry name" value="GMP SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 8" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="2 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="180"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="76"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="163"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002332"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01510"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96132.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="185" mass="21138" checksum="8510334723993CF0" modified="2013-06-26" version="1">MIVIINNGGQYVHRIYRSLKYLNIPSKIIPNENLEEMFNEIENAYGVIFSGGPSLERAKNCIEVALKVDKPILGICLGHQLIALAYGGEVGRAEKEEYALTEIYVDDEDDIFKNIPKKFNAWASHKDEVKKLPEDFVILAHSDICKVEAMKHKRKPIYGVQFHPEVAHTEYGDKILKNFYDVCIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ85</accession>
    <name>N6VZ85_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Small GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_02390</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96437.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590357.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ85"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00359"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.470"/>
    <dbReference type="OrthoDB" id="372125at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01896">
        <property type="entry name" value="DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01666">
        <property type="entry name" value="TGS_DRG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000016">
        <property type="entry name" value="Developmentally-regulated GTP-binding protein 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1070">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045001">
        <property type="entry name" value="DRG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031662">
        <property type="entry name" value="GTP-binding_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43127">
        <property type="entry name" value="DEVELOPMENTALLY-REGULATED GTP-BINDING PROTEIN 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16897">
        <property type="entry name" value="MMR_HSR1_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="3">
        <location>
            <begin position="61"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="4">
        <location>
            <begin position="289"/>
            <end position="364"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96437.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="366" mass="40897" checksum="723ABBA29469F631" modified="2013-06-26" version="1">MNVEEEIRRIEEELRRTPYNKATQKHIARLKAKLAKLREQAQGRKSGGGGKGYAVKKSGDATAAFVGFPSVGKSTLLNKLTNAKSEVGAYAFTTLTIVPGILEYKGAKIQLLDAPGIIAGASAGKGRGTEVLSAVRSADLILLTVDIFTLDHLPIIEKELYNVGIRLDQKPPDVKIKIKDRGGISVNSTVPLDIDEDEIRAILNEYKIHNADVIIREPITVEQFIDVVTGNRVYIPSLVVVNKIDLADENYIKYIEERLREFGKDYVLVSGNKGINLDLLKEKIYNKLGFIKIYLKPQGKKPDFDEPLIMRKGATVKDVCEKLHRDFVKNFRYALVWGKSVKHPGQRVGLDHKLEDEDILTIVIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZF5</accession>
    <name>N6VZF5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 19 20">Adenosylcobinamide-GDP ribazoletransferase</fullName>
            <ecNumber evidence="5 19 20">2.7.8.26</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 19">Cobalamin synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15 19">Cobalamin-5'-phosphate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">cobS</name>
        <name evidence="21" type="ORF">J422_01695</name>
    </gene>
    <organism evidence="21 22">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="21 22" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="21 22">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14 19">Joins adenosylcobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin). Also synthesizes adenosylcobalamin 5'-phosphate from adenosylcobinamide-GDP and alpha-ribazole 5'-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>alpha-ribazole + adenosylcob(III)inamide-GDP = adenosylcob(III)alamin + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:16049"/>
            <dbReference type="ChEBI" id="CHEBI:10329"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18408"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18 19">
            <text>alpha-ribazole 5'-phosphate + adenosylcob(III)inamide-GDP = adenosylcob(III)alamin 5'-phosphate + GMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23560"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57918"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:60487"/>
            <dbReference type="ChEBI" id="CHEBI:60493"/>
            <dbReference type="EC" id="2.7.8.26"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 19">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 19">Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 7/7.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Cell membrane</location>
            <topology evidence="2 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the CobS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="21">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.8.26" evidence="5 19 20"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96492.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590097.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZF5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00190"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.333"/>
    <dbReference type="OrthoDB" id="11748at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00238"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051073">
        <property type="term" value="F:adenosylcobinamide-GDP ribazoletransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008818">
        <property type="term" value="F:cobalamin 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00719">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003805">
        <property type="entry name" value="CobS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00317">
        <property type="entry name" value="cobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-GDP RIBAZOLETRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34148:SF1">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-GDP RIBAZOLETRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02654">
        <property type="entry name" value="CobS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 19" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="11 19" id="KW-0460">Magnesium</keyword>
    <keyword evidence="13 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="22" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="102"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="170"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="192"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="232"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00025228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00032853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00048623"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00049504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00719"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="NCBIfam" id="TIGR00317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="EMBL" id="ENN96492.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="252" mass="28064" checksum="A7248834F02CA243" modified="2013-06-26" version="1">MIRAFKLMLSFFTRFPINVEDFNFDTIAEHFYLVVIIGYIYGAISYFLSFLPLPPLLLSCIILFTIEFINGFHHFDGLLDFGEGLMVIADRKTKLKIMKDPYVGTFGLSFGVFYIITTVVSIYYILKINPLYLLAVEVLSKLSLLSCASFGNPLKEGTGKYFVKRADEKFLALALILSLPLLLIFDSTGRKAVSLAIIISVLLGLFMAKLAKGQFGGVNGDVLGATNEVSRVINLIAILFFLLTLEHNLSSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZH0</accession>
    <name>N6VZH0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF356 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01770</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96507.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590113.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZH0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00205"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.348"/>
    <dbReference type="OrthoDB" id="73585at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007154">
        <property type="entry name" value="DUF356"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04009">
        <property type="entry name" value="DUF356"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006606">
        <property type="entry name" value="UCP006606"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96507.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="119" mass="13391" checksum="1AF8AA42190D9ADA" modified="2013-06-26" version="1">MTLILIRGDSFEKLKNALADVDRHADLTIIGKPKIIVPEAADEILATILGEVKKPCKTACLAKIAEKAPKAIDRIRKIHPPAHIVVISERYGEIYYKLLDDFPKLPVLKGYYKSKKKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZJ9</accession>
    <name>N6VZJ9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01925</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96532.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590145.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZJ9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00233"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.379"/>
    <dbReference type="OrthoDB" id="65554at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96532.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="177" mass="20609" checksum="1AE28F6EDB5BC1AF" modified="2013-06-26" version="1">MIFDYDLIIATIILIVGLGMWSVSLVEHNNVYLDAVKNEYKIDKGLSTMETLIKSGVLQDFVILYSFNDTEIKRKAIMLLNNSIPLDSYRLEINNETIIEKNFNSSNTYIIATLILNRSEGWYIIYGNDTYIDILDKRFPDYMIAKEYAEKQLNYPILMPVYYSKNISSVIVKLYIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZX7</accession>
    <name>N6VZX7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Family 2 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_01071</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96642.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589882.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZX7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00829"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.208"/>
    <dbReference type="OrthoDB" id="11098at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04179">
        <property type="entry name" value="DPM_DPG-synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050256">
        <property type="entry name" value="Glycosyltransferase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48090:SF3">
        <property type="entry name" value="UNDECAPRENYL-PHOSPHATE 4-DEOXY-4-FORMAMIDO-L-ARABINOSE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48090">
        <property type="entry name" value="UNDECAPRENYL-PHOSPHATE 4-DEOXY-4-FORMAMIDO-L-ARABINOSE TRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5" id="KW-0448">Lipopolysaccharide biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="128"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="9">
        <location>
            <begin position="4"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022985"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96642.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="228" mass="26334" checksum="E851733326209E0A" modified="2013-06-26" version="1">MIAVIPAYNEERNIIKVLKDLNDLGIDAIVIDDGSTDNTYKVVEDFSKKAKIKIYLIKNEKNLGKAKSIKKGTELALSLGYELIIYMDGDYQHKPKDIIKLYNKLNKCNADAVFGVRKYKKIPFHRKIMNVIASFLTSLAIMVYTKKIIFFRDIQCGFRIIKANFLKDVEFGEGYAVEHLIALQLVKKGAKIVEEYVDVDYHDEAISYITHKKILNVIRQIIKFVFFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6W027</accession>
    <name>N6W027_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9">Translation initiation factor 2 subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 9">aIF2-beta</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8 9">eIF-2-beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">eif2b</name>
        <name evidence="11" type="ORF">J422_00646</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 9">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the eIF-2-beta/eIF-5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589807.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W027"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00905"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.124"/>
    <dbReference type="OrthoDB" id="38099at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.30.170:FF:000001">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.30.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00232">
        <property type="entry name" value="eIF_2_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045196">
        <property type="entry name" value="IF2/IF5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004458">
        <property type="entry name" value="TIF2_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002735">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016189">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016190">
        <property type="entry name" value="Transl_init_fac_IF2/IF5_Zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00311">
        <property type="entry name" value="aIF-2beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003067">
        <property type="entry name" value="PRK03988.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23001:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01873">
        <property type="entry name" value="eIF-5_eIF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00653">
        <property type="entry name" value="eIF2B_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100966">
        <property type="entry name" value="Translation initiation factor 2 beta, aIF2beta, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75689">
        <property type="entry name" value="Zinc-binding domain of translation initiation factor 2 beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9 11" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation initiation factor IF2/IF5" evidence="10">
        <location>
            <begin position="26"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010397"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00032408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00232"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="139" mass="16426" checksum="9434239308A12539" modified="2013-06-26" version="1">MEIDYYDYKALLKRAREQIPEYVYQKDRFELPEIELLIEGNRTIIRNFKELAKAMNRDMEMLAKYLFKETGSAGSIEGNRLILQRRINPELLKARINDFLREYVLCKECGKPDTKIIKEGRIHLLKCTACGAIRPIRMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTC7</accession>
    <name>N6UTC7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PfkB domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_07172</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000078">
        <property type="protein sequence ID" value="ENN95559.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004595165.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTC7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01006"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1385"/>
    <dbReference type="OrthoDB" id="26949at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033785">
        <property type="term" value="F:heptose 7-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033786">
        <property type="term" value="F:heptose-1-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016773">
        <property type="term" value="F:phosphotransferase activity, alcohol group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01172">
        <property type="entry name" value="RfaE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011913">
        <property type="entry name" value="RfaE_dom_I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46969">
        <property type="entry name" value="BIFUNCTIONAL PROTEIN HLDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46969:SF1">
        <property type="entry name" value="BIFUNCTIONAL PROTEIN HLDE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="3">
        <location>
            <begin position="2"/>
            <end position="294"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95559.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="304" mass="34123" checksum="FF6C3EF695D4CD3C" modified="2013-06-26" version="1">MIVVLGEVMLDKYTYGNVERISPEAPVPVLNAIEEKYTLGGAGNVANNIASLKHDVFLISIINRDEFGKCIEKLCNENGIKYYFISDGRPTIVKHRFVAIGYNQQLLRVDYEKIYPINEEISNKILDKIKRLNSKSNILIISDYAKGLITKELMEKIKKEFKGKILVDPKPKNIEFYKDVYLIKPNLKETSQIIGREIENKDNIIEKIGLELVEEFNSNFVITRSEKGSTLVTLNGEVYHISTKVKEVHDVSGAGDTFIAVLAYALDKGYDLVDAVKLANKASSIVVGKVGTATISLDELFNDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTN3</accession>
    <name>N6UTN3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06386</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95694.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593522.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTN3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01297"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1243"/>
    <dbReference type="OrthoDB" id="350214at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016472">
        <property type="entry name" value="Tscrpt_reg_MJ0621_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005978">
        <property type="entry name" value="HTH_MJ0621_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="21"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95694.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="170" mass="19161" checksum="FDF41545F7A84297" modified="2013-06-26" version="1">MKASERLLLKIEDSEKFVKEFKRILLELGLTLKEFAEISEIPYSTLYKIIKGKDFRVSTLVKIIKTIRQFEDKEDIDVIAVVAARPALNKITTKEIELDGKKYLIKEYPANSLEECIISAIRAEKDGVKGIICAPIVSSTIEKVVDIPVAVVIPDKNAFIEALKIITKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTQ7</accession>
    <name>N6UTQ7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Alkaline phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_06204</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the alkaline phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95724.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593336.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTQ7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00297"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1208"/>
    <dbReference type="OrthoDB" id="212977at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004035">
        <property type="term" value="F:alkaline phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16012">
        <property type="entry name" value="ALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001952">
        <property type="entry name" value="Alkaline_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018299">
        <property type="entry name" value="Alkaline_phosphatase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11596:SF5">
        <property type="entry name" value="ALKALINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00245">
        <property type="entry name" value="Alk_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00113">
        <property type="entry name" value="ALKPHPHTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00098">
        <property type="entry name" value="alkPPc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00123">
        <property type="entry name" value="ALKALINE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95724.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="493" mass="55031" checksum="554B6D8EED17CF7F" modified="2013-06-26" version="1">MKKISATLIIFGLIFGIMLCGCVNKANSEGITQNTNTYIKEVSFSKIGSNESKHKVKNVILLIGDGMGVTQAYIAERYKQEVENGNLVILTKFKNKGLITTYSESSEITDSAAAGTALLSGYKTYNGMINMKPDGSIPKKTLGELAKKMGKSVGIVTTTRITHATPASVYAHVKSREEEDEIAEQLLEFEPTVAFGGGLEYFIPKDMKDSKRKDNKNLIEEFKKKGYVVVFNKKELENIDINKTNKIIGLFAKSHMAYEVDRENIDKYKDQPSLAEMTEMALKILEKNPKGFFLMVEGGRIDHACHAHDAKSEIMDTIAFDNAVKVALDFQKKHPDTLIVVTADHETAGMSVGTGTEYYANITALKNINASIEYMIKEISKNSTKENILRLIKEHWGIELTDDEKALLFKKSPTSKINDSYLLSKYPKINKYVRNWAGFALSEIESRRAHIGWASFAHTAVPVPVYAIGPNSEIFNGFYDNTEVPEKIKEVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUI3</accession>
    <name>N6UUI3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATPase AAA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04620</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN95999.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUI3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00942"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="41"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95999.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="41" mass="4997" checksum="D3452123CCD4D925" modified="2013-06-26" version="1" fragment="single">MKIKKLGLSVEEIVRQWINIEKDRLVYLLKSNKERKENLLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUI8</accession>
    <name>N6UUI8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF22 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04665</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96004.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591957.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUI8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00951"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.912"/>
    <dbReference type="OrthoDB" id="69140at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002572">
        <property type="entry name" value="DUF22"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01629">
        <property type="entry name" value="DUF22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF22" evidence="1">
        <location>
            <begin position="14"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96004.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="123" mass="13740" checksum="C44492E83B6A3DE8" modified="2013-06-26" version="1">MFRVLGRLPSAIKMVKAKERIFKIKNENVKLVPIVADEDKEFKLGEVKPIKIKKIKIPPNHLTILCAYARHRLGHVIAIGEDIPLPLDAERSADMATFACGCDGVVKKNDLIGMLILVEVEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUN0</accession>
    <name>N6UUN0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PRC-barrel domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04203</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96044.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591716.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUN0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00681"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.822"/>
    <dbReference type="OrthoDB" id="68960at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.240">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027275">
        <property type="entry name" value="PRC-brl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011033">
        <property type="entry name" value="PRC_barrel-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38137">
        <property type="entry name" value="PRC-BARREL DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38137:SF1">
        <property type="entry name" value="PRC-BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05239">
        <property type="entry name" value="PRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50346">
        <property type="entry name" value="PRC-barrel domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PRC-barrel" evidence="1">
        <location>
            <begin position="3"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96044.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="89" mass="9855" checksum="B8C3744271531C82" modified="2013-06-26" version="1">MPIRVKDILDKHVYTTKAMYVGKVYDVMVDVENGVIKGLILSDISKGCLKDFVSDPTKKVVIPYGLITAIGNIILVKPPAENGYSFLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUV6</accession>
    <name>N6UUV6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Transcriptional regulator, ArsR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_04003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000025">
        <property type="protein sequence ID" value="ENN96134.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591458.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUV6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00732"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.784"/>
    <dbReference type="OrthoDB" id="14763at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052026">
        <property type="entry name" value="ExeA_AAA_ATPase_DNA-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35894">
        <property type="entry name" value="GENERAL SECRETION PATHWAY PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35894:SF1">
        <property type="entry name" value="PHOSPHORIBULOKINASE _ URIDINE KINASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="61"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="2">
        <location>
            <begin position="327"/>
            <end position="405"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96134.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="409" mass="46619" checksum="DC2C29B3BB33B9D7" modified="2013-06-26" version="1">MDPIEFIQQSACSIKNTMYKLKLKYNPFSEKPIRGNTKFFVGRVSELREIGEILGAAMHGSVANAAIVGTKGIGKSSMLNIIYYATKKQGHWVVELTASQITPRQFLIQLLYNILIENLISINGTIKSDYMEHSQRILEMYKRLNNYGDKVPIHYPRERIERDLEYLINDVKDEDKLCIILIDEADQFAKKSCLSLLQFFHSFLYEENILAFFAGSPTLMDDLTKISPPIKDRIPKIINMPPLSKDESYDLIRRRLEDAHIDGADTFEPFTEDAIDMIVEECDGIPRKIIMTCSESISIAIKKHLRKIDENIVKEALHHLGISVGHQILNHLTPAQSEIVKAMAKLGGAATVTQLAEYLNKSPSTIGTHLSDIYEMGYIYKERDKNNVYYKLSKELKDVLLGEDNELEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVA6</accession>
    <name>N6UVA6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Aspartate carbamoyltransferase regulatory chain</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pyrI</name>
        <name evidence="10" type="ORF">J422_03004</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in allosteric regulation of aspartate carbamoyltransferase.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Contains catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PyrI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96294.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVA6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00148"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.589"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009347">
        <property type="term" value="C:aspartate carbamoyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006221">
        <property type="term" value="P:pyrimidine nucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.20">
        <property type="entry name" value="Aspartate carbamoyltransferase regulatory subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.140">
        <property type="entry name" value="Aspartate carbamoyltransferase regulatory subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00002">
        <property type="entry name" value="Asp_carb_tr_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020545">
        <property type="entry name" value="Asp_carbamoyltransf_reg_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002801">
        <property type="entry name" value="Asp_carbamoylTrfase_reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020542">
        <property type="entry name" value="Asp_carbamoyltrfase_reg_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036792">
        <property type="entry name" value="Asp_carbatrfase_reg_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036793">
        <property type="entry name" value="Asp_carbatrfase_reg_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00240">
        <property type="entry name" value="ATCase_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35805:SF1">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE REGULATORY CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01948">
        <property type="entry name" value="PyrI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02748">
        <property type="entry name" value="PyrI_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57825">
        <property type="entry name" value="Aspartate carbamoyltransferase, Regulatory-chain, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54893">
        <property type="entry name" value="Aspartate carbamoyltransferase, Regulatory-chain, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aspartate carbamoyltransferase regulatory subunit N-terminal" evidence="8">
        <location>
            <begin position="3"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate carbamoyltransferase regulatory subunit C-terminal" evidence="9">
        <location>
            <begin position="98"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021764"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96294.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="145" mass="16711" checksum="9A84ACA8558BEB9B" modified="2013-06-26" version="1">MELKVRKIKDGTVIDHIDAGKALIVYKILNIPNHIPITLAINVPSKKKEKKDILKIEGFELKEKDINKISLISPDVTINIIKDWQVVKKLKPKIPDLIEGILKCTNPNCITHFEPVEGKFIVESKNPLKIRCYYCERLLEKIIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UW28</accession>
    <name>N6UW28_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">PKD domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_01910</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96529.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590140.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UW28"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00230"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.376"/>
    <dbReference type="OrthoDB" id="103676at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005261">
        <property type="term" value="F:monoatomic cation channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006816">
        <property type="term" value="P:calcium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00146">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022409">
        <property type="entry name" value="PKD/Chitinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46730:SF4">
        <property type="entry name" value="POLYCYSTIC KIDNEY DISEASE PROTEIN 1-LIKE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46730">
        <property type="entry name" value="POLYCYSTIN-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18911">
        <property type="entry name" value="PKD_4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="SMART" id="SM00089">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="983"/>
            <end position="1004"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="7">
        <location>
            <begin position="443"/>
            <end position="498"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="7">
        <location>
            <begin position="722"/>
            <end position="805"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="7">
        <location>
            <begin position="808"/>
            <end position="891"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="7">
        <location>
            <begin position="895"/>
            <end position="967"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96529.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="1010" mass="117503" checksum="F0469EBB14F0FBFF" modified="2013-06-26" version="1">MKKYLIIFLFIFLSSIFADVIFVHNGLMNITNSTGGDKFNINDTINFVALSIGNTTEIIIENGTVIWFFGDDKIKYGRNVNHSYTFPFIYPVAWGGYYKNVHYPPTETYNWLIVGDVYNTYYWFIPNNSLNTWVVEYYKNYTIANTSVNNSNVVIIKYPTDLDINDLIFKGLKVGGEVGIITDVNEDNIVIEDNITFIPNINKNNIEFIMWNFDNKTSFKFKPSIIYTTPGFHFIQVLIVDKYGKVWVGYKVIYVKQHFGGYVYWVMGPSHYGGEAYTYVYNSSLNHNDNRGNEYVNPHKLTYKVGDKTKFNMKDAWGLYWKWDFGDGVETDYTYREYFTPTEHVYKFPFMWPFFWMSYGGAGSWWKSDTLNFIIVGDVGNIKYNFYPNLSNSKTYKYEYDKKHHTVNLYYYSDIISNPVYNLTIRQGFYYDIPAEVDKPEVTTNKPVKFSFSYPGKVIFVMWCFGDGDISFESSPTHSYTEEGLYYPHVLIVDENGNIGLGIPPPIGVGYYSVPQIYVNPMITSTGKPVNITIVEPKRYTNLRHYIYYGDGNSTIITPGKSPYAWQYTYYKEGVYEVYMYISYNHRRFENSKKVYVINNNPPIASLLILPNPASYKDVIEFNPKNSYDPDANRKIPVYDHNGEIIDNYTIPKTSPMAMIYGYNLLVYNSSGNLVFNYTSNSLDPVYHKFPIGNYTAILTVWDGFGDKNKTEINFSVINKKPVAYFIYYPENPDVGQRIVLDASYSFDPEGKIIKYKWDFGDGSSLETYNPIVYHIYNKPGFYRIKLTVWDELNANNSYERYITVSGILANFTYNPKYPKANENILFIDTSRSYPTNTKIVYRKWDFGDGSVEVNKKEVNHKYNKPGVYIVTLYVKNNINKYGYTSKLIIVGGNESYPPIANFNYIINGLNVTFNASPSYDPDGRIIRYIWNFGDGSSLETYNPIVYHIYNNSGVYTVVLTVIDNSNLSDNCTRYIHLYEENIFSIPVPIYIHIMILILTFYFIRRYYEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWC6</accession>
    <name>N6UWC6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01373</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyltransferase 28 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589938.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWC6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00014"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.269"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03785">
        <property type="entry name" value="GT28_MurG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11190">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007235">
        <property type="entry name" value="Glyco_trans_28_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005262">
        <property type="entry name" value="MJ1255-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00661">
        <property type="entry name" value="MJ1255"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21015:SF22">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21015">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--N-ACETYLMURAMYL-(PENTAPEPTIDE) PYROPHOSPHORYL-UNDECAPRENOL N-ACETYLGLUCOSAMINE TRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04101">
        <property type="entry name" value="Glyco_tran_28_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyl transferase family 28 C-terminal" evidence="2">
        <location>
            <begin position="196"/>
            <end position="336"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04101"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="376" mass="43209" checksum="B6EBC7EA3167F072" modified="2013-06-26" version="1">MKILISVCGEGFGHTTRCLSIGKALEDSYDVAYLAYGKSYDFIKEHGYTVFKTYPEIKLKGEDGKFSIKSTVFNKEYSPRKAIERELKIIKEYKPDLIISDCKYSTIVVGKMLNKPIMCITNQNYTKYKFKTDIIVYPTMKFLNIIHKACEKIIIPDFPLPYTVCEYNLKLLNNMEFIGPLIRYNVDTVNDGNYILSVIGGFEYRYKILESLAEIAVKGGYNVKLVCGSYEVAKKLKNKYGDYKNIDIIPLAINMKGLIANASLIVSHGGHSTIMEAISFGKPLIVIPDLDHPEQNNNAKKVDDLKCGIYLSYYELNRLGEAIKEIEENEIYKKNAEKLMNLAKNYYNGKENIRRLIDNFFNNKFFRKKIFNVIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWD6</accession>
    <name>N6UWD6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">DEAD/DEAH box helicase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_01253</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589914.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWD6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00039"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.245"/>
    <dbReference type="OrthoDB" id="27512at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016818">
        <property type="term" value="F:hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006555">
        <property type="entry name" value="ATP-dep_Helicase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045028">
        <property type="entry name" value="DinG/Rad3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014013">
        <property type="entry name" value="Helic_SF1/SF2_ATP-bd_DinG/Rad3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006554">
        <property type="entry name" value="Helicase-like_DEXD_c2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472">
        <property type="entry name" value="DNA REPAIR DEAD HELICASE RAD3/XP-D SUBFAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11472:SF34">
        <property type="entry name" value="REGULATOR OF TELOMERE ELONGATION HELICASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13307">
        <property type="entry name" value="Helicase_C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00488">
        <property type="entry name" value="DEXDc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00491">
        <property type="entry name" value="HELICc2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51193">
        <property type="entry name" value="HELICASE_ATP_BIND_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="6" id="KW-0234">DNA repair</keyword>
    <keyword evidence="10" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="8">
        <location>
            <begin position="6"/>
            <end position="255"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="7">
        <location>
            <begin position="28"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="9">
        <location>
            <begin position="447"/>
            <end position="631"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="646" mass="75970" checksum="974A016B10D02054" modified="2013-06-26" version="1">MDFIEYIKEKFPYKNIRNPQKKMMFKIYNSIINRKHLIIEAPTGVGKTLAYLIPSLYFAERGKRIAILTETIDQQLRIYEDLKTLKHSIKVSFFMGKHNYYCKSKKGKANRLYCQLNRKCIYKPNRKPICYCGASKIMIKFGEKILYYCPFCTCEYQKNKIESVLADIVVMNNSIFYHSKEELEKKRGFDIIICDEAHKLEDSIRRSATIEINFDNIINRLKYMAMYYAPSLLKKRLNPESDRFWEIIENYLESKNVMLDICRSDIIYNGVLTSNYYNLNLSILGAILDGYKQILGIREIVNSFDDNKEIDKKDLYFDFYSPSLINIEMDFVENKNLDLYIAEFIENINNLRFIDENYVVYKNKEQLICEPVFINHVLKSLYADATVIHCSATIGNIKLHAYKTGLSEYDYLILENPFPRDRRMIIALKDGVNMKYEIKDRDKANYILLKLLEAINGNTLILFKSFEDLYNFHNYLKENIRKINIKNKNIHVYEQGMDGKDAKELKERFIEIGGIMLATGRFSEGVDIPGEALIGVIIDALPFPVPTPLLMREQSYLMERGFSKWQSFLMTSFDKMAKTLIQMIGRLIRTENDYGVVVIQDQRFYRWVGKVMKEKGYLKEDYSVMGLDEALKEIPKFINKFRSPYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6V013</accession>
    <name>N6V013_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">7-carboxy-7-deazaguanine synthase</fullName>
            <shortName evidence="8">CDG synthase</shortName>
            <ecNumber evidence="8">4.3.99.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Archaeosine biosynthesis protein QueE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">queE</name>
        <name evidence="10" type="ORF">J422_06897</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the complex heterocyclic radical-mediated conversion of 6-carboxy-5,6,7,8-tetrahydropterin (CPH4) to 7-carboxy-7-deazaguanine (CDG), a step common to the biosynthetic pathways of all 7-deazapurine-containing compounds.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>6-carboxy-5,6,7,8-tetrahydropterin + H(+) = 7-carboxy-7-carbaguanine + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:27974"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:61032"/>
            <dbReference type="ChEBI" id="CHEBI:61036"/>
            <dbReference type="EC" id="4.3.99.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </cofactor>
        <text evidence="8">Binds 1 S-adenosyl-L-methionine per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="8">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; 7-cyano-7-deazaguanine biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the radical SAM superfamily. 7-carboxy-7-deazaguanine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.99.3" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594264.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V013"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01068"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1338"/>
    <dbReference type="OrthoDB" id="7980at2157"/>
    <dbReference type="UniPathway" id="UPA00391"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016840">
        <property type="term" value="F:carbon-nitrogen lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904047">
        <property type="term" value="F:S-adenosyl-L-methionine binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00917">
        <property type="entry name" value="QueE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024924">
        <property type="entry name" value="7-CO-7-deazaguanine_synth-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42836">
        <property type="entry name" value="7-CARBOXY-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42836:SF1">
        <property type="entry name" value="7-CARBOXY-7-DEAZAGUANINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0408">Iron</keyword>
    <keyword evidence="6 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="9">
        <location>
            <begin position="15"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="9"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="24"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="34"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="233" mass="27265" checksum="267A2EEEAC42D71E" modified="2013-06-26" version="1">MIREIFNSIMGEGKYIGRRFIFVRFAGCPLECIYCDENIKNYLNRVEKTPGSGDFIEEELGLEEIVYHIKRLKTPDVFAISFTGGEPLLYHKEIRKIVSNLDEITFLETNGIFPNRVFYFDIASVDIKLREHFNDIKNYNKIYRNELKTIKKFYNMGSDVYAKVVILKNTKLERLREVAKDLCDIGDITLCIQPVFNKDLKPSNKKLFEILKICGEYVDVMLTVQMHKYMGVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6V021</accession>
    <name>N6V021_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">UDP-glucuronate 4-epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06758</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000059">
        <property type="protein sequence ID" value="ENN95638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594058.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V021"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00984"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1313"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43574">
        <property type="entry name" value="EPIMERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01713">
        <property type="entry name" value="NUCEPIMERASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="6"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="326" mass="38194" checksum="06296F4A537FBB66" modified="2013-06-26" version="1">MKYKNILVTGSAGFIGFHLSKYLLENYDDVNVIGIDNLNNYYKPILKEKRNEILKNYENYHFIKLDFSDWNSLFENLKNMEIDLIVHLGAQAGVRYSLENPWAYIKSNDMGTLNIFELARKLDIEKVVYASSSSVYGGNKKIPFSEDDRVDKPISLYAATKRSNELMAYTYHHLYGIKMIGLRFFTVYGEYGRPDMAFWKFTKNILLGKPIEVYNYGKMERDFTYISDIVDGIIKSIDKNFDYEIFNLGNDNPTNLEYAISLIEKYLGKKAIKDYKPIQPGDVERTWADLTKSRKLLGYDPKVRIEEGLKRFCEWFLKNKDWTLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6V058</accession>
    <name>N6V058_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-phosphoglycerate kinase</fullName>
            <shortName evidence="5">2PGK</shortName>
            <ecNumber evidence="5">2.7.2.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pgk2</name>
        <name evidence="8" type="ORF">J422_06346</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the phosphorylation of 2-phosphoglycerate to 2,3-diphosphoglycerate. Involved in the biosynthesis of cyclic 2,3-bisphosphoglycerate, a thermoprotectant.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(2R)-2-phosphoglycerate + ATP = (2R)-2,3-bisphosphoglycerate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42408"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58248"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.16"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Thermoadapter biosynthesis; cyclic 2,3-diphosphoglycerate biosynthesis; cyclic 2,3-diphosphoglycerate from 2-phospho-D-glycerate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the 2-phosphoglycerate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.16" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95688.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593510.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V058"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01304"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1235"/>
    <dbReference type="OrthoDB" id="358692at2157"/>
    <dbReference type="UniPathway" id="UPA00551">
        <property type="reaction ID" value="UER00609"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016774">
        <property type="term" value="F:phosphotransferase activity, carboxyl group as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00769">
        <property type="entry name" value="2PGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020872">
        <property type="entry name" value="2PKG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003259">
        <property type="entry name" value="PRK04220.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477">
        <property type="entry name" value="P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33477:SF3">
        <property type="entry name" value="P-LOOP NTPASE DOMAIN-CONTAINING PROTEIN LPA1 HOMOLOG 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-cone" evidence="7">
        <location>
            <begin position="2"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95688.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="310" mass="35822" checksum="D6BF6407D80E9E8E" modified="2013-06-26" version="1">MEDIIVKGKNYKMPFSKGILARSLTAAGLKPSIAYKISWDIYENLKKENIKEIEKSELRRRVYYYLISHNYDEIAERYLLWRVFLEKKPLTILIGGASGVGTSTIAFEIASRLGISSVIGTDSIREVMRKIISKDLVPTLYESSYTAWKVLRDFNEGDTKKLYIRGFERHAESVLVGVEGVIDRAMVEGLNLIIEGVHLVPSLLKPKYLDNPYIIFIMLTVEDEDMHKMRFYARSKISERPKERYLKHFKIIRLINDYLVDNAKKMGIPVINNVSISKTVESCLNIITDRLREVIKFEGLNEDELIDEVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6V074</accession>
    <name>N6V074_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphomethylpyrimidine kinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06466</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593550.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V074"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01282"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1259"/>
    <dbReference type="OrthoDB" id="43786at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008902">
        <property type="term" value="F:hydroxymethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008972">
        <property type="term" value="F:phosphomethylpyrimidine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01169">
        <property type="entry name" value="HMPP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.225.10">
        <property type="entry name" value="Class II aldolase/adducin N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036409">
        <property type="entry name" value="Aldolase_II/adducin_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004399">
        <property type="entry name" value="HMP/HMP-P_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013749">
        <property type="entry name" value="PM/HMP-P_kinase-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019293">
        <property type="entry name" value="ThiN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858:SF17">
        <property type="entry name" value="HYDROXYMETHYLPYRIMIDINE_PHOSPHOMETHYLPYRIMIDINE KINASE THI20-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20858">
        <property type="entry name" value="PHOSPHOMETHYLPYRIMIDINE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08543">
        <property type="entry name" value="Phos_pyr_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10120">
        <property type="entry name" value="ThiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53639">
        <property type="entry name" value="AraD/HMP-PK domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyridoxamine kinase/Phosphomethylpyrimidine kinase" evidence="1">
        <location>
            <begin position="9"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="Thiamine-phosphate synthase ThiN" evidence="2">
        <location>
            <begin position="237"/>
            <end position="394"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08543"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="403" mass="45776" checksum="15A55560765B3556" modified="2013-06-26" version="1">MLLIVGGFDPISGAGISVDIKTANTLNIYTPSIITCTVPQTNKEVYEKFEIPEDNIKKQFKAVFEEFDIKIVKTGVLSKDAIDILVKYIKKYNLKVICDPVLSSTTGYNFIDVKDYKKLFELSYLITPNKEEYEKIKDFNLENYILITGIDDTLIKGNKKIYEFKGYKIDKEVHGTGCCYATAIACFLYKGYDLIEAIKEAKKLVLASVIYANKSRYGYNVNPTYINKEKVLKNLYYALKLLKKFNFSLIPEVGSNIAESLILPKDINDIASLTGRIVKNKENSFYIAGDFKFGVNGHIARVVLSASKINPKVRACMNIRYDEDLIDRLKDNFKITSFNRKDEPKNVSTMEWGVSVALKKDKEADIIYDTGDVGKEPMIRVLGKDAIDVVNKVKEIEKIYKKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>N6V081</accession>
    <name>N6V081_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Transcription factor E</fullName>
            <shortName evidence="4">TFE</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">TFIIE subunit alpha homolog</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Transcription initiation factor TFIIE</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">tfe</name>
        <name evidence="7" type="ORF">J422_06301</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Transcription factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Facilitates transcription initiation by enhancing TATA-box recognition by TATA-box-binding protein (Tbp), and transcription factor B (Tfb) and RNA polymerase recruitment. Not absolutely required for transcription in vitro, but particularly important in cases where Tbp or Tfb function is not optimal. It dynamically alters the nucleic acid-binding properties of RNA polymerases by stabilizing the initiation complex and destabilizing elongation complexes. Seems to translocate with the RNA polymerase following initiation and acts by binding to the non template strand of the transcription bubble in elongation complexes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer. Interaction with RNA polymerase subunits RpoF and RpoE is necessary for Tfe stimulatory transcription activity. Able to interact with Tbp and RNA polymerase in the absence of DNA promoter. Interacts both with the preinitiation and elongation complexes.</text>
    </comment>
    <comment type="domain">
        <text evidence="4">The winged helix domain is involved in binding to DNA in the preinitiation complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the TFE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000051">
        <property type="protein sequence ID" value="ENN95718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593434.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V081"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01273"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1226"/>
    <dbReference type="OrthoDB" id="5935at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006367">
        <property type="term" value="P:transcription initiation at RNA polymerase II promoter"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01909">
        <property type="entry name" value="TFE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016481">
        <property type="entry name" value="TF_E_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039997">
        <property type="entry name" value="TFE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017919">
        <property type="entry name" value="TFIIE/TFIIEa_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002853">
        <property type="entry name" value="TFIIE_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024550">
        <property type="entry name" value="TFIIEa/SarR/Rpc3_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004910">
        <property type="entry name" value="PRK06266.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00373">
        <property type="entry name" value="transcription factor E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097:SF7">
        <property type="entry name" value="GENERAL TRANSCRIPTION FACTOR IIE SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13097">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIE, ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02002">
        <property type="entry name" value="TFIIE_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006373">
        <property type="entry name" value="TF_E_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00531">
        <property type="entry name" value="TFIIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51344">
        <property type="entry name" value="HTH_TFE_IIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH TFE/IIEalpha-type" evidence="6">
        <location>
            <begin position="8"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00373"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="179" mass="21684" checksum="86C521D3E767AA8B" modified="2013-06-26" version="1">MKIRKMLNDPLVQEVLFNIFEGDEKGFELIEVLLKKGETTEEELAKELGIKLNYVRKLLYKLYDARLVDYKKWKNEDSNWFYYTWRPTFEKIPYVVKKRVKELITDLENKLKYEKNNMFFYCPKCNIRFTFEEATDLNFLCPGCGNMLEEFDNSKIIKDLEEQIKFLEHELKHNPILNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0A1</accession>
    <name>N6V0A1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Aminotransferase</fullName>
            <ecNumber evidence="4">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_06065</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 4">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95743.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593227.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0A1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00532"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1181"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050881">
        <property type="entry name" value="LL-DAP_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004937">
        <property type="entry name" value="PRK06290.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42832">
        <property type="entry name" value="AMINO ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42832:SF3">
        <property type="entry name" value="L-GLUTAMINE--4-(METHYLSULFANYL)-2-OXOBUTANOATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4 6" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="5">
        <location>
            <begin position="45"/>
            <end position="369"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95743.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="416" mass="47678" checksum="2D1056D8D369CA5B" modified="2013-06-26" version="1">MSFIQELFAERIGGRKFGKEEVIYKFEKIKRAKREAMKKHPDMELIDMGVGEPDEMADREVIEVLYREALKWENRGYADNGIQELKDAVPPYMERVYGVKDIDPVNEVIHSIGSKSALAYLPSAFINPGDYVLMTVPGYPVLATHAEWYGGKVYKLPLLEENNFLPDLESVPKDVRERAKILYINYPNNPTGAQATKKFYKEVVDFAFENNIIVVNDAAYGALVYDGKPLSFLSVKDAKEVGVEIHSFSKAFNMTGWRLAFLVGNELIIKAFATVKDNFDSGQFIPIQKAGIYCLQHPEITERIRKKYERRLRKMVKIMNEAGFKARMPGGTFYLYVKSPKRANGREFKTAEEFSEFLIKEKLISTVPWDDAGAYIRLSATFVAKDENGNPTTEEKYEDKILEEFKNRLLTLDLEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0D7</accession>
    <name>N6V0D7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Binding-protein-dependent transport systems inner membrane component</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_05998</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593056.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0D7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00559"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1168"/>
    <dbReference type="OrthoDB" id="11163at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30183">
        <property type="entry name" value="MOLYBDENUM TRANSPORT SYSTEM PERMEASE PROTEIN MODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30183:SF3">
        <property type="entry name" value="MOLYBDENUM TRANSPORT SYSTEM PERMEASE PROTEIN MODB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 9" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="79"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="174"/>
            <end position="198"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="210"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="41"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="240" mass="26831" checksum="5EBCDE02B14A12F1" modified="2013-06-26" version="1">MKVGYVTLLALTLFILLPLLYLITNPGDLSQLNDHEVINAFKTTILAGIIATIIGLILSLPSGYILARKDFRFKSFVEAILDLPMAIPHSVIGIMLLSFIYGISFFDWLREYIVDNFWGIVIVYLFVGLPFLINSIRDGFILIDEEIENVAKTLGASEFRIFFDILLPLIKNNIISGSILCFARGVSEVGAILIIAYYPKTVPILILERFLSFGLLASKPISVAMVIVCLILFTVLRRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0H4</accession>
    <name>N6V0H4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Polyferredoxin MvhB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_05788</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0H4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01248"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1128"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10549">
        <property type="entry name" value="MtMvhB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050157">
        <property type="entry name" value="PSI_iron-sulfur_center"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24960">
        <property type="entry name" value="PHOTOSYSTEM I IRON-SULFUR CENTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24960:SF85">
        <property type="entry name" value="POLYFERREDOXIN PROTEIN VHUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="8"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="37"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="80"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="109"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="136" mass="15720" checksum="05767E67E574A145" modified="2013-06-26" version="1">MNIIFNKREIIVREDKCLKCLECLIVCPTGAIEEGKYIVEINKNKCVFCGRCEKTCKFNAISIIKLRFENFKITEVKKYEDIYINYNRCVGCLVCLKNCPFNAITTKNDKIDIIKDKCNLCGKCEEVCPSEAIKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0J1</accession>
    <name>N6V0J1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">(5-formylfuran-3-yl)methyl phosphate synthase</fullName>
            <ecNumber evidence="5">4.2.3.153</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">4-(hydroxymethyl)-2-furancarboxaldehyde-phosphate synthase</fullName>
            <shortName evidence="5">4-HFC-P synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mfnB</name>
        <name evidence="7" type="ORF">J422_05578</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 5">Catalyzes the formation of 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate (4-HFC-P) from two molecules of glyceraldehyde-3-P (GA-3-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4 5">
            <text>2 D-glyceraldehyde 3-phosphate = 4-(hydroxymethyl)-2-furancarboxaldehyde phosphate + phosphate + 2 H2O</text>
            <dbReference type="Rhea" id="RHEA:43536"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:83407"/>
            <dbReference type="EC" id="4.2.3.153"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; methanofuran biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the MfnB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.153" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592674.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0J1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00278"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1088"/>
    <dbReference type="OrthoDB" id="81473at2157"/>
    <dbReference type="UniPathway" id="UPA00080"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016830">
        <property type="term" value="F:carbon-carbon lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001120">
        <property type="term" value="P:methanofuran biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00945">
        <property type="entry name" value="Aldolase_Class_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00681">
        <property type="entry name" value="MfnB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007565">
        <property type="entry name" value="4HFCP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035081">
        <property type="entry name" value="4HFCP_synth_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002573">
        <property type="entry name" value="PRK02227.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002575">
        <property type="entry name" value="PRK02227.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04476">
        <property type="entry name" value="4HFCP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015957">
        <property type="entry name" value="UCP015957"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="5 6">
        <location>
            <position position="27"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="5 6">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047628"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR015957-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="235" mass="25331" checksum="7C5F62F8BA767AD3" modified="2013-06-26" version="1">MLLLVSPINKEEAIEAIEGGADIIDVKNPKEGSLGANFPWIIREIREVTPRHLLVSATVGDVPYKPGTVSLAALGAAMSGADYIKVGLYGVKNYNQAVDLMKHVVKAVKDYDADKIVVAAGYADAYRVNAVDPLIIPKIARDSGADVAMIDTAIKDGKTLFDFLNKEILEEFVDESHSYGLKCALAGSIKKEHIPILKSIKTDIVGVRGAACKGGDRNSGRIDRELVRELKSLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0L5</accession>
    <name>N6V0L5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">tRNA-splicing endonuclease</fullName>
            <ecNumber evidence="4">4.6.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">tRNA-intron endonuclease</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">endA</name>
        <name evidence="7" type="ORF">J422_05534</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Endonuclease that removes tRNA introns. Cleaves pre-tRNA at the 5'- and 3'-splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini. Recognizes a pseudosymmetric substrate in which 2 bulged loops of 3 bases are separated by a stem of 4 bp.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>pretRNA = a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus.</text>
            <dbReference type="EC" id="4.6.1.16"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homotetramer; although the tetramer contains four active sites, only two participate in the cleavage. Therefore, it should be considered as a dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the tRNA-intron endonuclease family. Archaeal short subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.16" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000042">
        <property type="protein sequence ID" value="ENN95858.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592610.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0L5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00747"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1080"/>
    <dbReference type="OrthoDB" id="46045at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000213">
        <property type="term" value="F:tRNA-intron lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22363">
        <property type="entry name" value="tRNA-intron_lyase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1350.10:FF:000006">
        <property type="entry name" value="tRNA-splicing endonuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1170.20">
        <property type="entry name" value="tRNA intron endonuclease, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01833">
        <property type="entry name" value="EndA_short"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036167">
        <property type="entry name" value="tRNA_intron_Endo_cat-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006677">
        <property type="entry name" value="tRNA_intron_Endonuc_cat-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006678">
        <property type="entry name" value="tRNA_intron_Endonuc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036740">
        <property type="entry name" value="tRNA_intron_Endonuc_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006676">
        <property type="entry name" value="tRNA_splic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016442">
        <property type="entry name" value="tRNA_splic_arch_short"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00324">
        <property type="entry name" value="endA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21227:SF0">
        <property type="entry name" value="TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01974">
        <property type="entry name" value="tRNA_int_endo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02778">
        <property type="entry name" value="tRNA_int_endo_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005285">
        <property type="entry name" value="tRNA_splic_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53032">
        <property type="entry name" value="tRNA-intron endonuclease catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55267">
        <property type="entry name" value="tRNA-intron endonuclease N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 4" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA intron endonuclease N-terminal" evidence="6">
        <location>
            <begin position="6"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA intron endonuclease catalytic" evidence="5">
        <location>
            <begin position="78"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="107"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="117"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00024798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02778"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95858.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="171" mass="19896" checksum="59D279D2E7AF27BD" modified="2013-06-26" version="1">MRKILGMLDKDRVVIFDKNGINKLLSKHYGNFEGNFLSLSLVEALYLVNLGWLEVKDKKILSFEELYNYAKNIEERLCLKYLTYKDLRSRGYIVKTGLKYGADFRLYERGANIEKEHSAYLVKVLSEDDRITVNELTGFVRVAHSVRKKLLIAIVDNDGDIVYYNMAFIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0Y4</accession>
    <name>N6V0Y4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit Rpo3</fullName>
            <ecNumber evidence="5">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpo3</name>
        <name evidence="5" type="synonym">rpoD</name>
        <name evidence="7" type="ORF">J422_04955</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the archaeal Rpo3/eukaryotic RPB3 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95963.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592097.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0Y4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00499"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.971"/>
    <dbReference type="OrthoDB" id="84933at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.120.12">
        <property type="entry name" value="DNA-directed RNA polymerase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00320">
        <property type="entry name" value="RNApol_arch_Rpo3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001514">
        <property type="entry name" value="DNA-dir_RNA_pol_30-40kDasu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011262">
        <property type="entry name" value="DNA-dir_RNA_pol_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011263">
        <property type="entry name" value="DNA-dir_RNA_pol_RpoA/D/Rpb3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022842">
        <property type="entry name" value="RNAP_Rpo3/Rpb3/RPAC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036643">
        <property type="entry name" value="RNApol_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050518">
        <property type="entry name" value="Rpo3/RPB3_RNA_Pol_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011548">
        <property type="entry name" value="PRK14979.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11800:SF2">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01000">
        <property type="entry name" value="RNA_pol_A_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01193">
        <property type="entry name" value="RNA_pol_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00662">
        <property type="entry name" value="RPOLD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56553">
        <property type="entry name" value="Insert subdomain of RNA polymerase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00446">
        <property type="entry name" value="RNA_POL_D_30KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RpoA/D/Rpb3-type" evidence="6">
        <location>
            <begin position="15"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00662"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95963.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="200" mass="22647" checksum="C1A419769FBBDED3" modified="2013-06-26" version="1">MLTIKEKKQTKIGEEFIFSLKAPISFCNAIRRIMISEVPTYAIEDVYIYENTSSMDDEILAHRLGLIPIKGKPASDKEVITFTLEKEGPCTVYSSDLKSENGEVAFKNIPIVKLGEKQKIKIVCEAVPGIGKVHAKWQPCNAVYRIKDDEVEFFVETFGQMDGEEILKEAIKILKEKAFSFLHQLELLGDKDEKADENKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>N6V110</accession>
    <name>N6V110_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 14">Mevalonate kinase</fullName>
            <shortName evidence="14">MK</shortName>
            <shortName evidence="14">MVK</shortName>
            <ecNumber evidence="3 14">2.7.1.36</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="primary">mvk</name>
        <name evidence="17" type="ORF">J422_05055</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Catalyzes the phosphorylation of (R)-mevalonate (MVA) to (R)-mevalonate 5-phosphate (MVAP). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds such as archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>(R)-mevalonate + ATP = (R)-5-phosphomevalonate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17065"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:36464"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="13 14">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway; isopentenyl diphosphate from (R)-mevalonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the GHMP kinase family. Mevalonate kinase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.36" evidence="3 14"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95983.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592139.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V110"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00478"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.991"/>
    <dbReference type="OrthoDB" id="19001at2157"/>
    <dbReference type="UniPathway" id="UPA00057">
        <property type="reaction ID" value="UER00098"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004496">
        <property type="term" value="F:mevalonate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.890">
        <property type="entry name" value="GHMP kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00217">
        <property type="entry name" value="Mevalonate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036554">
        <property type="entry name" value="GHMP_kinase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006203">
        <property type="entry name" value="GHMP_knse_ATP-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006205">
        <property type="entry name" value="Mev_gal_kin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022937">
        <property type="entry name" value="Mevalonate_kinase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00549">
        <property type="entry name" value="mevalon_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43290:SF2">
        <property type="entry name" value="MEVALONATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00959">
        <property type="entry name" value="MEVGALKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55060">
        <property type="entry name" value="GHMP Kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00627">
        <property type="entry name" value="GHMP_KINASES_ATP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="12 14" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="8 14 17" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 14" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="11 14" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 14" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="15">
        <location>
            <begin position="63"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="16">
        <location>
            <begin position="211"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="14">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="91"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00029438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN95983.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="297" mass="33767" checksum="0C9EE454E9FD614A" modified="2013-06-26" version="1">MVAKVILFGEHAVVYGYRAISMAINLNTSVKVEKADKFIIYSENLNKTLVSDNLECNDRDFSYIINTLKIFFNNFNIEPEYLKLSIYSEIPVSCGLGSSASVIIETLKSLSNFYNIELDKNDIFKLAYEVERITQGKASITDTATITYGGVLEIKDNKFRKLNDLNDFIKECDFLIAYVEKRNKKTADLVKEVSNLDYKDEIFKEIDKIAKEAIKVNDKKEFGELLLKNHELLKKLNISTNKIDKVIDIAKKYSYGAKITGAGGGGCVIILLNDNWKELIKRIKNHVISLYPCKVIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6V116</accession>
    <name>N6V116_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Peptidase M50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_05080</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95988.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592148.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V116"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00473"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.996"/>
    <dbReference type="OrthoDB" id="15212at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031293">
        <property type="term" value="P:membrane protein intracellular domain proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.42.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001193">
        <property type="entry name" value="MBTPS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036034">
        <property type="entry name" value="PDZ_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008915">
        <property type="entry name" value="Peptidase_M50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325:SF3">
        <property type="entry name" value="MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE-2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13325">
        <property type="entry name" value="PROTEASE M50 MEMBRANE-BOUND TRANSCRIPTION FACTOR SITE 2 PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02163">
        <property type="entry name" value="Peptidase_M50"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01000">
        <property type="entry name" value="SREBPS2PTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50156">
        <property type="entry name" value="PDZ domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="129"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="170"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="265"/>
            <end position="284"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="290"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="337"/>
            <end position="356"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M50" evidence="6">
        <location>
            <begin position="107"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95988.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="357" mass="40156" checksum="3F9476BD99290809" modified="2013-06-26" version="1">MKIVALIFIIIWVFLLAVREKINLKVYFGAFGILRTKIGLNVIDKLGKYKFWRKIGYISIPICVLLGGYALFNIINMSIKLISGELPKSAGKPIIFLFGSVIPILPGIIALTIAITAHELFHGVIARSFNINVKSSGILLMLGFPLGFFVELDEKFKTTEKKVRAAIASAGPMANLILCVISLPLMYLSYSIPSEIVVIKTYEPASKYLKSGDIILRINDYNIKSIADFKNFAKEIKPNKVYRIEVLRGNKVISYNIKSSNEGKLGLLVSPSGSLAFLINTLYWSYLFNLLLALFNLLPAFPLDGFYVFSSIPEIFKSIRLYKFGNFLEVIVNEKTLRSISLLIWWIILGAIIYSMW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1H2</accession>
    <name>N6V1H2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04128</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000027">
        <property type="protein sequence ID" value="ENN96118.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591626.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1H2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01349"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.808"/>
    <dbReference type="OrthoDB" id="63425at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019300">
        <property type="entry name" value="CooT"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10133">
        <property type="entry name" value="CooT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96118.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="56" mass="6667" checksum="0807BAB61E356C28" modified="2013-06-26" version="1">MCSCNLYFKNELVMEDVIFVKKENNKIIAYDLFGEKKEFEGDIKIVDLNNNKIYIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1N7</accession>
    <name>N6V1N7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="14">Peptidase M50</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">J422_03613</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cell membrane</location>
            <topology evidence="2">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M50B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96203.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591257.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1N7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01381"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.705"/>
    <dbReference type="OrthoDB" id="86131at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06158">
        <property type="entry name" value="S2P-M50_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052348">
        <property type="entry name" value="Metallopeptidase_M50B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044537">
        <property type="entry name" value="Rip2-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35864">
        <property type="entry name" value="ZINC METALLOPROTEASE MJ0611-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35864:SF1">
        <property type="entry name" value="ZINC METALLOPROTEASE YWHC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="5" id="KW-0645">Protease</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 13" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="10 13" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="71"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="115"/>
            <end position="142"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="13">
        <location>
            <begin position="162"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN96203.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="187" mass="20994" checksum="BB52BB986F193838" modified="2013-06-26" version="1">MYKFSKEEIRDLIGSVIVISIIFIYPHISITLFLIALIAVGSGFIFHELAHRFVARYYGAFSMFKAWYEGLLLALILKIVLGVTLIAPGAVYIYKDHLTNKENGVIAISGPLTNILLAFIFLIISFIPIPLISLTGTVGFYINLYLAAFNLLPIPPLDGYKVVTWNPIIWAIVEIPLVIYILFRGIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1R2</accession>
    <name>N6V1R2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Polysaccharide deacetylase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03488</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000022">
        <property type="protein sequence ID" value="ENN96228.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591148.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1R2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01336"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.680"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.370">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051398">
        <property type="entry name" value="Polysacch_Deacetylase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34216">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34216:SF3">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE N-DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96228.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="271" mass="31798" checksum="0B0A2F82C8E1416A" modified="2013-06-26" version="1">MKFIITIDDAEKETMNILLRENIDIPVVIGVPVGLLGKSLGDRNIASIDLIRDLIMNNSTIELASHGYYHIPPPSCRTKLFLLKKFLKNMILSLDKKEYLIRSLCFARSEIGKNTNEGSTTEYLQKFNPIDEIVTSKKLLEELFGTRIITYIYPGGNYTEELISLVSKYYNFARTTDEGWNVIGDLYKEPNKFLLKSISISRYTNFLKLEKRYEKMKNEERAEEIIVIETYHDFTYKMPRRLYQINFNVFKQHYNKLRNIGELTTFRTLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1S1</accession>
    <name>N6V1S1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">type I site-specific deoxyribonuclease</fullName>
            <ecNumber evidence="3">3.1.21.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">J422_03428</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.</text>
            <dbReference type="EC" id="3.1.21.3"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HsdR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.3" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000021">
        <property type="protein sequence ID" value="ENN96238.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591064.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1S1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00939"/>
    <dbReference type="REBASE" id="68612">
        <property type="entry name" value="MviT80ORF3443P"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.670"/>
    <dbReference type="OrthoDB" id="11429at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120545">
        <property type="term" value="F:nucleic acid conformation isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009035">
        <property type="term" value="F:type I site-specific deoxyribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18030">
        <property type="entry name" value="DEXHc_RE_I_HsdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22332">
        <property type="entry name" value="HsdR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18800">
        <property type="entry name" value="SF2_C_EcoR124I-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1570.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055180">
        <property type="entry name" value="HsdR_RecA-like_helicase_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007409">
        <property type="entry name" value="Restrct_endonuc_type1_HsdR_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004473">
        <property type="entry name" value="Restrct_endonuc_typeI_HsdR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040980">
        <property type="entry name" value="SWI2_SNF2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021810">
        <property type="entry name" value="T1RH-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051268">
        <property type="entry name" value="Type-I_R_enzyme_R_subunit"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00348">
        <property type="entry name" value="hsdR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30195:SF15">
        <property type="entry name" value="TYPE I RESTRICTION ENZYME HINDI ENDONUCLEASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30195">
        <property type="entry name" value="TYPE I SITE-SPECIFIC DEOXYRIBONUCLEASE PROTEIN SUBUNIT M AND R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04313">
        <property type="entry name" value="HSDR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18766">
        <property type="entry name" value="SWI2_SNF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22679">
        <property type="entry name" value="T1R_D3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11867">
        <property type="entry name" value="T1RH-like_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0540">Nuclease</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0680">Restriction system</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="12">
        <location>
            <begin position="300"/>
            <end position="463"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="882"/>
            <end position="926"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012654"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96238.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="1014" mass="118766" checksum="695AB2A1525D6A82" modified="2013-06-26" version="1">MVEALNEPQTVENLAIEIFKKLGYNYKHGSKLTPENKERPTYRDVILKNRFISAIKKLNPWLNDKLAEEVYKIVSNIDHPDPIIRGKIFYDMLISGVKITYKESSNEKTRIVKLIDFSNPYNNDFLISNQFEVEYYKENNKYRIPDLVVFINGIPIAIFEFKGFKSDYTAKDAFEDHKIKKEDIPQLYQYAQILVASDGLETRYGSPTSDWERFFVWEGIESDDDVIVIETEEKTIYEYKCKTLTSLEVLLLGLFDKRRLIEYIEDFIIYDKSGKEYIKKIATYYQFYTVKKAIERTIKAVKGKDKRIGVVWHTQGSGKSLTMLFYAKKALKVKELENPILLFITDRRELDEQLYGIFKNVFPTAKHIETIKELQETLKKAKSGIFFATIQKFGRKNKEEYPFLTDKNNIIVVVDEAHRSQYRQLAQNLRKAIPNASFIGFTATPIDYRDRSTTLVFGDYISIYSIDKAKRHGVVVPIYYEARCIELHLTNEFIDLEFEEISENLIPEEKEEIKRAFAKLERIMLTDEYLDKISKDIVEHFNKRVTDLDGKAMVVTISRKVAVELYKRITKHPNAPSVAVVISGNKSKDPKEFHDHIRSKKELEELARKFKDPNSDPKMVIVVDMWLTGFDVPCLHTMYFWKPMKNHSLVQAIARVNRVFKNKPGGLIVDYIGISDDLKKSLSKYTSEQIKEVLTNIEEIVALLKEKYKIVSLYFKDLNYKNWKHLSSEELAHLTAKAYEMIAKDEKIKEEFLKNFIALKKLYLLASPHPEAIKIREDLAFFEMIKKMLVKYSIRKIKELSKEIEKDMSRLISKSVSASDPIDVFELLNKDKPDISIFSDEFLDKIKNIEYKNLAVDMLIKILNDNLKVKTRTNPWRYKSLYDRLKELIDKHNRKIIETSEMILKLIEIAKDIKKAEDEGKKLNLNEEEYTFYSLLLSYPNLPIEEKEKIKDIAKEIAKTLSGYIKVSDWKRNKFIKSKIKAEVKRILMKKGILEYSILNKLSNIIVEHAKVME</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1U5</accession>
    <name>N6V1U5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL16</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 5" type="primary">rpl10e</name>
        <name evidence="5" type="ORF">J422_03353</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96263.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590973.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1U5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01056"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.657"/>
    <dbReference type="OrthoDB" id="30538at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01433">
        <property type="entry name" value="Ribosomal_L16_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1170.10:FF:000008">
        <property type="entry name" value="50S ribosomal protein L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.10">
        <property type="entry name" value="Ribosomal protein L10e/L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00448">
        <property type="entry name" value="Ribosomal_uL16_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047873">
        <property type="entry name" value="Ribosomal_uL16"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022981">
        <property type="entry name" value="Ribosomal_uL16_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018255">
        <property type="entry name" value="Ribosomal_uL16_CS_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016180">
        <property type="entry name" value="Ribosomal_uL16_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001197">
        <property type="entry name" value="Ribosomal_uL16_euk_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036920">
        <property type="entry name" value="Ribosomal_uL16_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003236">
        <property type="entry name" value="PRK04199.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003239">
        <property type="entry name" value="PRK04199.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00279">
        <property type="entry name" value="uL16_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11726">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00252">
        <property type="entry name" value="Ribosomal_L16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005590">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54686">
        <property type="entry name" value="Ribosomal protein L16p/L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01257">
        <property type="entry name" value="RIBOSOMAL_L10E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96263.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="174" mass="19567" checksum="A76C1EDDCFCACE76" modified="2013-06-26" version="1">MAALRPNRCYRDVDKPPYTRKEYIKGVPQPKVVHYIMGNLSADFPVQVDLVATRPIQIRHNALEAARVAANKYISKMCGRLGYKFQIRVYPHQVLREHKMATGAGADRISDGMRLAFGKPIGTAARVKEGQAIMSIWVNPDKYEAAKEALRRAAMKLPIPCRIVVAKGRELLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1Y2</accession>
    <name>N6V1Y2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Cobyrinic acid a,c-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_03024</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96298.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590749.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1Y2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00152"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.593"/>
    <dbReference type="OrthoDB" id="26717at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004484">
        <property type="entry name" value="CbiA/CobB_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004921">
        <property type="entry name" value="PRK06278.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873:SF2">
        <property type="entry name" value="COBYRIC ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="COBYRINATE A,C-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobB/CobQ-like glutamine amidotransferase" evidence="2">
        <location>
            <begin position="36"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96298.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="468" mass="53274" checksum="D11DD1382654301E" modified="2013-06-26" version="1">MDIGILDIKGALPLFEDFGNLPTKIIDEKNYKEIRDLLALIIPGGSLVESRSFPEEIKREIIDFDGYIIGICSGFQILGKRVDVGRKSHPKIIECLGLLDVEFSPLVCTDRVKFKINNKVFGSGIGEGFHCHTYGNIKILDKKTEVLTVSEVEKLNYKINKKEIISGAFKGKVFGTMVHNFLDNLFIRDNFLNYLNITDEEREEIFEKNKLIKEELKKRALIYKINKKYENNGVKKKRGILILSTSSNSGKTFLTTAIVSKLCGKVFVAKIGGDVRDIVPSLYLIREPMTKYNSIRIGKSGWVSVEEFVDFIKNSNYDYIIIEGVMGAFTGAMKGISSYQIARKLNFPVYIVATCNLSGIEGAFVEALSYYFILKDILDVKGIILNRVYDFNLFEKLKNLAENYGVKLYAVEKVDEKNIPEIEIDYESFCLNASKLNINIEIPEIEIKLNEEEDNFLLSVEKFIRRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2D0</accession>
    <name>N6V2D0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Adenosylmethionine-8-amino-7-oxononanoate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02579</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96423.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590479.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2D0"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.505"/>
    <dbReference type="OrthoDB" id="6534at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43094">
        <property type="entry name" value="AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43094:SF1">
        <property type="entry name" value="AMINOTRANSFERASE CLASS-III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96423.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="108" mass="12516" checksum="CE70EEEF198CA2D7" modified="2013-06-26" version="1" fragment="single">KERVIEKIQPKIKLFHELLRELKDLEHVGDVRGRGFMVGIELVKDKETKEPYPYGYKAGFRVAEALLKRNIYMRPIGNVIILVPPLSITEEEIEYLCSSLYDAIKEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6V318</accession>
    <name>N6V318_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 3">GTP cyclohydrolase III</fullName>
            <ecNumber evidence="2 3">3.5.4.29</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">gch3</name>
        <name evidence="4" type="ORF">J422_01051</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 3">Catalyzes the formation of 2-amino-5-formylamino-6-ribofuranosylamino-4(3H)-pyrimidinone ribonucleotide monophosphate and inorganic phosphate from GTP. Also has an independent pyrophosphate phosphohydrolase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>GTP + 3 H2O = 2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22468"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57258"/>
            <dbReference type="EC" id="3.5.4.29"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 3">Belongs to the archaeal-type GTP cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.29" evidence="2 3"/>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96638.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589878.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V318"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00834"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.204"/>
    <dbReference type="OrthoDB" id="25211at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043740">
        <property type="term" value="F:GTP cyclohydrolase IIa activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1230">
        <property type="entry name" value="Nucleotide cyclase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00608">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007839">
        <property type="entry name" value="GTP_CycHdrlase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029787">
        <property type="entry name" value="Nucleotide_cyclase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002587">
        <property type="entry name" value="PRK02240.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42202">
        <property type="entry name" value="GTP CYCLOHYDROLASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42202:SF1">
        <property type="entry name" value="GTP CYCLOHYDROLASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05165">
        <property type="entry name" value="GCH_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009265">
        <property type="entry name" value="GTP_cyclohydro_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR009265"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96638.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="264" mass="30196" checksum="96B97C39F4156C55" modified="2013-06-26" version="1">MIQITVIQIDNYGPWTVTPKPRRESDLQALQSRLYSDLNLLFGAHKGLVFYTRFDNLIAITNGIDLITHKRIQENIRNRYPFTISMVIASGETPYEAQKLATETLQEYGSAKDENRREVLDVANELIDGYVQIAHIDINNITSYTDTLSTYDNYLNIKRVQLLLMEELLKHEALLFFVGGDNFMSPCNGMNEEDFLDIFDKVYKKLNFHLKAGIGVGKTAEDASNLADIGLEKIRAREVNKSVCFLKQDFIENPTFKRKICGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3A5</accession>
    <name>N6V3A5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">D-3-phosphoglycerate dehydrogenase</fullName>
            <ecNumber evidence="3 10">1.1.1.95</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_00841</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>(2R)-3-phosphoglycerate + NAD(+) = 3-phosphooxypyruvate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12641"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18110"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="EC" id="1.1.1.95"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 10">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.95" evidence="3 10"/>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589838.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3A5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00866"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.166"/>
    <dbReference type="OrthoDB" id="7437at2157"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00196"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004617">
        <property type="term" value="F:phosphoglycerate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04902">
        <property type="entry name" value="ACT_3PGDH-xct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd12173">
        <property type="entry name" value="PGDH_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.90:FF:000003">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000021">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000008">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase, chloroplastic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.90">
        <property type="entry name" value="D-3-phosphoglycerate dehydrogenase, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029009">
        <property type="entry name" value="ASB_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050857">
        <property type="entry name" value="D-2-hydroxyacid_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006139">
        <property type="entry name" value="D-isomer_2_OHA_DH_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029753">
        <property type="entry name" value="D-isomer_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029752">
        <property type="entry name" value="D-isomer_DH_CS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006140">
        <property type="entry name" value="D-isomer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006236">
        <property type="entry name" value="PGDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045626">
        <property type="entry name" value="PGDH_ASB_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01327">
        <property type="entry name" value="PGDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42789:SF1">
        <property type="entry name" value="D-ISOMER SPECIFIC 2-HYDROXYACID DEHYDROGENASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_6G10090)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00389">
        <property type="entry name" value="2-Hacid_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02826">
        <property type="entry name" value="2-Hacid_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19304">
        <property type="entry name" value="PGDH_inter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143548">
        <property type="entry name" value="Serine metabolism enzymes domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00065">
        <property type="entry name" value="D_2_HYDROXYACID_DH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00670">
        <property type="entry name" value="D_2_HYDROXYACID_DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00671">
        <property type="entry name" value="D_2_HYDROXYACID_DH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="domain" description="ACT" evidence="11">
        <location>
            <begin position="450"/>
            <end position="522"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005854"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00021582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363003"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="523" mass="57492" checksum="A5C9E0EA8BDBDEDD" modified="2013-06-26" version="1">MVKILITDPIHEEAIKILKELGEVEIATDLSKEELLNKIKDVNILIVRSGTYVDREIIDAGKNLKIIARAGVGVDNIDVDYATEKGIIVVNAPDASSISVAELTMGLILAAARNIPQANNSVKSGKWERKKFKGIELYGKILGVVGLGRIGQQVVKRAKAFGMEVIAYDPYIPKEVAENLGVKLLNDLNELCKMADIITLHVPLTPKTKHMIGEEQINLMKKNAIIVNCARGGLIDEKALYKALKEKRIKAAALDVFEKEPPKDNPLLKLDNLICTPHLGASTEEAQKAAGIIIAEQIKKILNGELAENVVNFPSLPKEKLGKLKPYMLLAETLGNIVMQVLNRVDRVEIIYMGSLTKENTDLITRAFLKGLLYPILLAGVNLINAPIIAKSRNINVIESYTTEKKYGDAIKIIAEGDRRFSIVGGIINNKFVILEVDGYEVNFIPEGVLAIIKHIDRPGMIGKVGMILGEYGINIASMQVGRREPGGEAVMLINLDHPISEEVIKKIKEIPNIKDVAVINLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3C1</accession>
    <name>N6V3C1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Probable [NiFe]-hydrogenase-type-3 Eha complex membrane subunit A</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_00536</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">One of the integral membrane subunits of multisubunit membrane-bound [NiFe]-hydrogenase eha. Eha is predicted to form large electron transfer complex and might catalyze energy-driven reduction of low-potential redox carriers.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Putative multisubunit membrane-bound [NiFe]-hydrogenase eha is composed of at least 20 subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EhaA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589789.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3C1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00059"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.103"/>
    <dbReference type="OrthoDB" id="81652at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011306">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17367">
        <property type="entry name" value="NiFe_hyd_3_EhaA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="5"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="82" mass="9222" checksum="9C994EFFD3CA8EE6" modified="2013-06-26" version="1">MGYILAIIVSMIFAYLFKLPLIPKSKFSFQTSIIFPTPIIALGFYVIFKYLFFDSISFSIISGIIGAVFAKYSNKIFGVCND</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3C5</accession>
    <name>N6V3C5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(NiFe)-hydrogenase-3-type complex Eha, membrane protein EhaF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00561</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589794.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3C5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00064"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.108"/>
    <dbReference type="OrthoDB" id="116966at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011313">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaF"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09879">
        <property type="entry name" value="EhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019373">
        <property type="entry name" value="EhaF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="18"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="140"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="143" mass="16818" checksum="3A7A2E755C11DCA0" modified="2013-06-26" version="1">MIGKIWNYLSKPEVVPRIFALFLAIFLLFGFFNHYYKIPNQLYPKPKPHSQILKTPLAPYDRNIVKAQYPNYLKNLGKITSYLTPIAEFIKDKTYFFGTTIVSTPGGILDEILYYTRGMDTVLESTVLLISFMIFSWMFFHRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3D8</accession>
    <name>N6V3D8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger SWIM domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00411</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589764.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3D8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00698"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.79"/>
    <dbReference type="OrthoDB" id="41163at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007527">
        <property type="entry name" value="Znf_SWIM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04434">
        <property type="entry name" value="SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50966">
        <property type="entry name" value="ZF_SWIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="SWIM-type" evidence="2">
        <location>
            <begin position="39"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="215" mass="25738" checksum="83E06F1F0FCEE71B" modified="2013-06-26" version="1">MYDERIKERGKNYYINGLVKYCIKLGNKLYGKVVGSDIYNVEVDLKTLEGVCSCPYRYNCKHAYALILAYKNNDYVDGDSLFNELKKRDKEEIINILKNIVVNNYLWETFLEKESLGDKALKLIKLINYDKKAIYTFLSFLRNNVKNLSNKELLKIIKAMLDYDIYYYEEHYLEALELIINEILKRDDEDTIEELLKLYNKHKNELWLIEELLFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3E3</accession>
    <name>N6V3E3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Cobyrinate a,c-diamide synthase</fullName>
            <ecNumber evidence="8">6.3.5.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Cobyrinic acid a,c-diamide synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ni-sirohydrochlorin a,c-diamide synthase</fullName>
            <ecNumber evidence="8">6.3.5.12</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Ni-sirohydrochlorin a,c-diamide synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cbiA</name>
        <name evidence="8" type="synonym">cfbB</name>
        <name evidence="11" type="ORF">J422_00436</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of cobyrinate, using either L-glutamine or ammonia as the nitrogen source. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the ATP-dependent amidation of the two carboxylate groups at positions a and c of Ni-sirohydrochlorin, using L-glutamine or ammonia as the nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Ni-sirohydrochlorin + 2 L-glutamine + 2 ATP + 2 H2O = Ni-sirohydrochlorin a,c-diamide + 2 L-glutamate + 2 ADP + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:52896"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:136841"/>
            <dbReference type="ChEBI" id="CHEBI:136887"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.12"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>cob(II)yrinate + 2 L-glutamine + 2 ATP + 2 H2O = cob(II)yrinate a,c diamide + 2 L-glutamate + 2 ADP + 2 phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:26289"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58537"/>
            <dbReference type="ChEBI" id="CHEBI:58894"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 10/10.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">Comprises of two domains. The C-terminal domain contains the binding site for glutamine and catalyzes the hydrolysis of this substrate to glutamate and ammonia. The N-terminal domain is anticipated to bind ATP, and cobyrinate or Ni-sirohydrochlorin, and catalyzes the ultimate synthesis of the diamide product. The ammonia produced via the glutaminase domain is probably translocated to the adjacent domain via a molecular tunnel, where it reacts with an activated intermediate.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="8">The a and c carboxylates of cobyrinate and Ni-sirohydrochlorin are activated for nucleophilic attack via formation of a phosphorylated intermediate by ATP. CbiA catalyzes first the amidation of the c-carboxylate, and then that of the a-carboxylate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the CobB/CbiA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.11" evidence="8"/>
    <dbReference type="EC" id="6.3.5.12" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96783.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589769.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3E3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00693"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.84"/>
    <dbReference type="OrthoDB" id="8896at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00231"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042242">
        <property type="term" value="F:cobyrinic acid a,c-diamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05388">
        <property type="entry name" value="CobB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03130">
        <property type="entry name" value="GATase1_CobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00027">
        <property type="entry name" value="CobB_CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004484">
        <property type="entry name" value="CbiA/CobB_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00379">
        <property type="entry name" value="cobB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033195">
        <property type="entry name" value="F430_CfbB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002204">
        <property type="entry name" value="PRK01077.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873">
        <property type="entry name" value="COBYRINATE A,C-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43873:SF1">
        <property type="entry name" value="COBYRINATE A,C-DIAMIDE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="7 8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="3 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="9">
        <location>
            <begin position="4"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="domain" description="CobB/CobQ-like glutamine amidotransferase" evidence="10">
        <location>
            <begin position="246"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <feature type="site" description="Increases nucleophilicity of active site Cys" evidence="8">
        <location>
            <position position="421"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96783.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="439" mass="49817" checksum="58DFE44B05A9BC0E" modified="2013-06-26" version="1">MKRVVIAGTSSEVGKTTIATGIMKALSKKYNVQGYKVGPDYIDPTYHTIATGNYSRNLDSFFMNKKQIKYLFQKHSKNKDISIIEGVRGLYEGLSIKDDTGSTASIAKILDAPVILIVNAKSLTKSAIAIIRGFMELDKDVKIKGVIFNFVRGEGHINKLKEAMQYYLPNIEIIGFIPRKEGLKLESRHLGLIPTPENMEFKNKIEKIGEIIEEYLDIDKIIDIADEDFDEVDEVYLWEIEENYKKVAIAYDEAFNFYYYDNFDALKENKAELIFFSPLRDEMPDADVLYIGGGYPEIFKEKLSKNKELIEQIREFDGYIYAECGGLMYLTKAIDDCKMVGLIDAKAIMTDKVQGLSYVKAKILEDCIVGKKDRTIKGHEFHYSKLIGLKDKRFAFKILRGRGIGGYDGIIKKNVLAGYLHNHAVANPYFASSMVNFEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP16</accession>
    <name>N6VP16_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_07117</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000074">
        <property type="protein sequence ID" value="ENN95575.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594971.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP16"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01213"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1377"/>
    <dbReference type="OrthoDB" id="61906at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="2">
        <location>
            <begin position="21"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="195"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="2">
        <location>
            <begin position="29"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95575.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="229" mass="26230" checksum="ED25A3F408652FFF" modified="2013-06-26" version="1">MGDNDLINRIKHLENLAMNLSKKLNSNSNEEKDEAKEEKKENSNEDKIKELREKLDSLKSECASGKIYERLEVILKKLDEGMSIIKNLDEQLKELSKKIEGIKPQEIGEDTINKITERVKEINESTKSEIIEKLNNIEKAYFGCTTKLDDIIDKLNNILDKIQAILESGGVKVVDIINLIKEIHSGISTVKELILKSKEEEIIKAIEIVDNLNNKIDNYIDEIKKEIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP23</accession>
    <name>N6VP23_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">tRNA 2-selenouridine synthase AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_07022</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000068">
        <property type="protein sequence ID" value="ENN95585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594739.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP23"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00920"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1360"/>
    <dbReference type="OrthoDB" id="61220at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043828">
        <property type="term" value="F:tRNA 2-selenouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058840">
        <property type="entry name" value="AAA_SelU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030815">
        <property type="entry name" value="Arch_SelU_Cterm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017582">
        <property type="entry name" value="SelU"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04569">
        <property type="entry name" value="arch_SelU_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30401">
        <property type="entry name" value="TRNA 2-SELENOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30401:SF0">
        <property type="entry name" value="TRNA 2-SELENOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26341">
        <property type="entry name" value="AAA_SelU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="tRNA 2-selenouridine synthase AAA" evidence="1">
        <location>
            <begin position="6"/>
            <end position="126"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF26341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="218" mass="25685" checksum="B6291B46E82E68F5" modified="2013-06-26" version="1">MIIFGLFGKTGCGKTEILRELKKMGHSVIDIEDIAKTKGSVLGDLYHLKMRSQEEFDKIINEEIKKAEKRGYVIVEYEGRKIGGVKKLEIPKIFAEVKNYNYKILIDCPYECQIRRLLELYKPKNEWEKEILKSRFLILKNSLKKEEILGAIDKIIELIDREEYLEAVKLAEERIYRVHYLRAIKKITPDLIVYNKDVKKSAEIIDDFVKKKLREIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPE6</accession>
    <name>N6VPE6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">RNA 2',3'-cyclic phosphodiesterase</fullName>
            <shortName evidence="2">RNA 2',3'-CPDase</shortName>
            <ecNumber evidence="2">3.1.4.58</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_06135</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Hydrolyzes RNA 2',3'-cyclic phosphodiester to an RNA 2'-phosphomonoester.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + H2O = a 3'-end 2'-phospho-ribonucleotide-RNA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11828"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:17353"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:173113"/>
            <dbReference type="EC" id="3.1.4.58"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the 2H phosphoesterase superfamily. ThpR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.58" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593254.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPE6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00546"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1195"/>
    <dbReference type="OrthoDB" id="44091at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004113">
        <property type="term" value="F:2',3'-cyclic-nucleotide 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008664">
        <property type="term" value="F:RNA 2',3'-cyclic 3'-phosphodiesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1140.10">
        <property type="entry name" value="Cyclic phosphodiesterase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01940">
        <property type="entry name" value="RNA_CPDase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009097">
        <property type="entry name" value="Cyclic_Pdiesterase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014051">
        <property type="entry name" value="Phosphoesterase_HXTX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004175">
        <property type="entry name" value="RNA_CPDase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02258">
        <property type="entry name" value="2_5_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561">
        <property type="entry name" value="RNA 2',3'-CYCLIC PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35561:SF1">
        <property type="entry name" value="RNA 2',3'-CYCLIC PHOSPHODIESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02834">
        <property type="entry name" value="LigT_PEase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55144">
        <property type="entry name" value="LigT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoesterase HXTX" evidence="3">
        <location>
            <begin position="7"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Phosphoesterase HXTX" evidence="3">
        <location>
            <begin position="91"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 1" evidence="2">
        <location>
            <begin position="38"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="HXTX 2" evidence="2">
        <location>
            <begin position="118"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="2">
        <location>
            <position position="118"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01940"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02834"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="173" mass="20423" checksum="A57FD3844C1666F8" modified="2013-06-26" version="1">MRLFLAIDIPKDIKNKIYEFQKSFKIKGIKLVEKENLHITVKFLGEVDENLLNKILDLDLSIEPIRIKLKNLGVFPNENFIRVIWVGVEGDKIIDLFKDIDKKLSTLGFKKENNYIPHLTIGRVKFIENKKLLKDRIREFKNVEFGSFTAEKIKLYKSTLTREGPIYEIIKEW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPJ0</accession>
    <name>N6VPJ0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Molybdate/tungstate import ATP-binding protein WtpC</fullName>
            <ecNumber evidence="8">7.3.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">J422_05723</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tungstate(in) + ATP + H2O = tungstate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35027"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.6" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592796.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPJ0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01260"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1116"/>
    <dbReference type="OrthoDB" id="10909at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901238">
        <property type="term" value="F:ABC-type tungstate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03293">
        <property type="entry name" value="ABC_NrtD_SsuB_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000425">
        <property type="entry name" value="Probable ABC transporter, ATP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050166">
        <property type="entry name" value="ABC_transporter_ATP-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42788:SF13">
        <property type="entry name" value="ALIPHATIC SULFONATES IMPORT ATP-BINDING PROTEIN SSUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42788">
        <property type="entry name" value="TAURINE IMPORT ATP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="4"/>
            <end position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN95810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="255" mass="29031" checksum="62FFEA12F6384457" modified="2013-06-26" version="1">MIKLKIEGLTKVFDVDGKKITALDDINLEVYKNEFLTVVGPSGCGKTTLLRIIAGLDKPTKGRILLDGKEIKGPGADRGVVFQQYTLLPWRNVIDNIAFGLELRGVPKKERYEIARKFIKLMGLEGFEKAYPYQLSGGMQQRVAIARTLANDPEIVLMDEPFAALDAQTRAILQNELLKIWQKDKKTIFFITHSIDEAVYLSDRVVVLTARPGKIKDIIEIPLERPRDKTSVEFLEYKKKIFEILKEEVLKTLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPJ3</accession>
    <name>N6VPJ3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Mur ligase middle domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_05748</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592806.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPJ3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01255"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1121"/>
    <dbReference type="OrthoDB" id="52890at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.10">
        <property type="entry name" value="Mur-like, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036565">
        <property type="entry name" value="Mur-like_cat_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051046">
        <property type="entry name" value="MurCDEF_CellWall_CoF430Synth"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033197">
        <property type="entry name" value="F430_CfbE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43024">
        <property type="entry name" value="UDP-N-ACETYLMURAMOYL-TRIPEPTIDE--D-ALANYL-D-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43024:SF1">
        <property type="entry name" value="UDP-N-ACETYLMURAMOYL-TRIPEPTIDE--D-ALANYL-D-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53623">
        <property type="entry name" value="MurD-like peptide ligases, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 4" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="381" mass="43897" checksum="E0C2B6FEF616AB17" modified="2013-06-26" version="1">MLIVDVNHGALILAREYKEMGYDVEVWDIYNKLKDSNNLDVKIVKNPNLNDYEKIVAPIHCPIDKKFISFHEAVSEIILKKYKNLYKKFICITGVKGKTTTTELIFHILKEDYKVFINNSNYGSIAPPAILEKLSNINIDDYDIFIFETSLGLIRCKYGAITNVLENYKIAKGKRDALFAKFSTLKLADVGYINIKDIKRYNLAIEKNNINVINEPTILKKYPLEFEYCGEIFNFNNKVFGSHLAENASFAIEICKNFLSLEDIKEKLKNFKIKNRMEIENVGKHVVIKNINPGLDVKAIKYAINDFLEVFGGDIYIGGDFGVVCEEIDIKKLCNILKSFKVKYFFIGDIGKELNKYLEGEFKDKIEIKDDTLIILRTRIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPP1</accession>
    <name>N6VPP1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Small ribosomal subunit protein eS19</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps19e</name>
        <name evidence="6" type="ORF">J422_05569</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">May be involved in maturation of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eS19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000042">
        <property type="protein sequence ID" value="ENN95865.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592618.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPP1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00755"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1087"/>
    <dbReference type="OrthoDB" id="371836at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000028">
        <property type="term" value="P:ribosomal small subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.10:FF:000449">
        <property type="entry name" value="30S ribosomal protein S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01474">
        <property type="entry name" value="Ribosomal_eS19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001266">
        <property type="entry name" value="Ribosomal_eS19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027548">
        <property type="entry name" value="Ribosomal_eS19_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018277">
        <property type="entry name" value="Ribosomal_eS19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006811">
        <property type="entry name" value="PRK09333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11710:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01090">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01413">
        <property type="entry name" value="Ribosomal_S19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00628">
        <property type="entry name" value="RIBOSOMAL_S19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01474"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95865.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="152" mass="17399" checksum="B085112B3337D1E9" modified="2013-06-26" version="1">MVTVYDVPADKLIQKTAEKLKEIGLKEPEWAKFVKTGVSRERRPQQDDWWYIRCASILRKVYINGPVGVSRLRTAYGGRKNRGHAPEHFYKGSGSIVRKALQELERLGLVEKRPEGRVISPKGRSFLDNLAKEVFDEVVKEKPELAKYGPGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ38</accession>
    <name>N6VQ38_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">ATPase AAA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04625</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96000.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ38"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96000.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="97" mass="11436" checksum="A4F79961B58E1CDC" modified="2013-06-26" version="1">MQLQQKELLEIFFEKGDKKYLLNKLEINLKIFKLGVEKNFDFNNLSLNDIFAKINESINAVIKEGKKPILIIDELQKLKNIYFNGLITIRSFKVLFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ57</accession>
    <name>N6VQ57_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription factor CBF/NF-Y/histone domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_04720</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds and compact DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils. Increases the resistance of DNA to thermal denaturation (in vitro).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96015.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591986.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ57"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00963"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.923"/>
    <dbReference type="OrthoDB" id="7514at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22909">
        <property type="entry name" value="HFD_archaea_histone-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050004">
        <property type="entry name" value="HmfB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF043032">
        <property type="entry name" value="archaea_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="8">
        <location>
            <begin position="2"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96015.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="97" mass="11364" checksum="A9B00786A92E7C53" modified="2013-06-26" version="1">MLPKTRIKKIMKQYTKLNISSEAVDELNNILLEIIKIITETAEKNAKREGRKTIKGRDIKECDDERLKRKIIELSKRTDKMPILIKEILNVITSELE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ98</accession>
    <name>N6VQ98_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_04268</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96055.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591738.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ98"/>
    <dbReference type="SMR" id="N6VQ98"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00669"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.835"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050157">
        <property type="entry name" value="PSI_iron-sulfur_center"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24960:SF79">
        <property type="entry name" value="PHOTOSYSTEM I IRON-SULFUR CENTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24960">
        <property type="entry name" value="PHOTOSYSTEM I IRON-SULFUR CENTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="26"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96055.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="54" mass="5968" checksum="7A53BA72B89089E9" modified="2013-06-26" version="1">MKVLDKCVGCGICVVFCKRRAIRTYGRAIIDKDKCNNCGICVKYCPINAIVLGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQC9</accession>
    <name>N6VQC9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Energy-converting hydrogenase B subunit P</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04343</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96070.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591770.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQC9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00654"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.851"/>
    <dbReference type="OrthoDB" id="73069at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019597">
        <property type="entry name" value="Energy-convert_hydgase-B_suP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10622">
        <property type="entry name" value="Ehbp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96070.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="94" mass="10981" checksum="7FE920686CCD184C" modified="2013-06-26" version="1">MPKMILYPKKVMALGGYIRETAFPYKDVEPFPYRNVIVANPLDEPIKIDVPAYDKDWVERHRKLGLIVVPVTEKDDFISLFLMVKEKIKKSEKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQG9</accession>
    <name>N6VQG9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0216 protein J422_04138</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04138</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0216 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000027">
        <property type="protein sequence ID" value="ENN96120.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQG9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01347"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.810"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00585">
        <property type="entry name" value="UPF0216"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002746">
        <property type="entry name" value="UPF0216"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003153">
        <property type="entry name" value="PRK04115.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01886">
        <property type="entry name" value="DUF61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005264">
        <property type="entry name" value="UCP005264"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96120.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="127" mass="14867" checksum="F9D71A7695DFF60B" modified="2013-06-26" version="1">MEKIIKSVIKDLNPRFKKKSLKELLNEDRPYILVNGKRHRIKRRELELLKEIASEDLKLPIVLEVDPSINAIVVKGKEEVKVVAKILGKEINIFSEENILYIYKPELRIVRRELPTATQLIFKLPLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQH3</accession>
    <name>N6VQH3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04163</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000027">
        <property type="protein sequence ID" value="ENN96125.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQH3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01342"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.815"/>
    <dbReference type="OrthoDB" id="383078at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="86"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="109"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="137"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96125.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="158" mass="18433" checksum="08866BD218282D97" modified="2013-06-26" version="1" fragment="single">PYALPGVEKRFLYLLIILGVIVIISYIQKLNKLLNFIINTLNKIFKPIISLSVGQKFVLLAIIFLIISAIDLILRGEHIANVDAIIAYYFLVIGVLNLLFEYWNESFQKLRIIVSLILLSVLIYYTPEVTKIYPKAYYLPIIILILFLVYQFLRKFYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQK7</accession>
    <name>N6VQK7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Protein translation factor SUI1 homolog</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_03943</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the SUI1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96175.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591397.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQK7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00418"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.773"/>
    <dbReference type="OrthoDB" id="11182at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002188">
        <property type="term" value="P:translation reinitiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11567">
        <property type="entry name" value="YciH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.780.10">
        <property type="entry name" value="SUI1-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00604">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050318">
        <property type="entry name" value="DENR/SUI1_TIF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001950">
        <property type="entry name" value="SUI1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022851">
        <property type="entry name" value="SUI1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005872">
        <property type="entry name" value="SUI1_arc_bac"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036877">
        <property type="entry name" value="SUI1_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002096">
        <property type="entry name" value="PRK00939.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01158">
        <property type="entry name" value="SUI1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789:SF0">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12789">
        <property type="entry name" value="DENSITY-REGULATED PROTEIN HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01253">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037511">
        <property type="entry name" value="Transl_init_SUI1_pro"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55159">
        <property type="entry name" value="eIF1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50296">
        <property type="entry name" value="SUI1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 4 5" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4 5" id="KW-0810">Translation regulation</keyword>
    <feature type="domain" description="SUI1" evidence="6">
        <location>
            <begin position="27"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005422"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRNR" id="PIRNR037511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96175.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="102" mass="11384" checksum="942BC22A66BC35BA" modified="2013-06-26" version="1">MPEICPKCGLPKELCVCEEIAKEEQKIKISVAKRRYGKLMTIIEGFDTSVINLKDLAKKLKDMCACGGTVKGNTIELQGDHRKKVAEILVKMGFSKDSIEIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQN0</accession>
    <name>N6VQN0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">GTP-binding HSR1-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_03598</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96200.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591252.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQN0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01384"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.702"/>
    <dbReference type="OrthoDB" id="372125at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01859">
        <property type="entry name" value="MJ1464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1580.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030378">
        <property type="entry name" value="G_CP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023179">
        <property type="entry name" value="GTP-bd_ortho_bundle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050755">
        <property type="entry name" value="TRAFAC_YlqF/YawG_RiboMat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089">
        <property type="entry name" value="GTP-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11089:SF30">
        <property type="entry name" value="GUANINE NUCLEOTIDE-BINDING PROTEIN-LIKE 3 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51721">
        <property type="entry name" value="G_CP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CP-type G" evidence="3">
        <location>
            <begin position="9"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96200.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="367" mass="42095" checksum="18BDC98C594D35B0" modified="2013-06-26" version="1">MRIKKVPVKKIVNKIINECDIILFVLDARDPELTRNKELEKRILNSGKKIIYVLNKADLVPKEILERWKRVFGENTVFVSAKQRLGTRILRDKIKKLLKEMGKKEGKVGVVGYPNVGKSSIINALTGSRKALTGSIAGLTKGEQWIRLTRNIRLLDTPGILEMKDEDDLIISGALRLEKVDNPIPAALKILKRIDNFDKSIVKDYFKVDYDKIDEKLLEKIGEKRGYIKKGGDVDLIRTSKAIIKEYQDGKLNYYKVNLKKYGQERSNDISFIMEYLKDFPFIEDAKMVITHLKDIEELKKKIKKPILGYEKVNDNVIIISFGEKTKDAGRKKIEKLCETENLEKISEFGGKVGKNNIYVAIAKKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQR4</accession>
    <name>N6VQR4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03438</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000021">
        <property type="protein sequence ID" value="ENN96240.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591068.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQR4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00937"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.672"/>
    <dbReference type="OrthoDB" id="359247at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96240.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="551" mass="64687" checksum="2ADD2C2F580F940C" modified="2013-06-26" version="1">MQIYFWSKSATKPQNKMEDKLYFNEVTTDEGGGILDLIQALEKKIGNWRDFNNDVDPENASVKKWIKKILESYPDRSDDEVEYLIDTFRAALNTKSKEADKFLIGVLQMENILVIVHCRKDPSLAEIKDKLYAVRVLLHPKNIIRADIIKKDGENIILGAFEYSRRMSKGHAKFWGIEPEDVGWESIGTIRLNVELEEFDFPIQIPIEQDDLKNLIDKGIISPTGRIKIGRSEGKVTKVFVHNKAYEYKMFYDMFVALTERLTEYRRRFEKLIPTQQKISMYFSNDRKYQYMEDEKAVYSFTQDREELKFKKEHPKYIVCFFTKSTPGIEPKHSFLLKLYQSIFEDTRELEIFHAGEEISLEPFKIGGLKIYNQVDISEDVVKFSENLMKQIKDTQSRKGKVLLEYLFCKVYSENIRNPHLKSLFEFIIENIIKAEIEYEFRNSGILQAENILEFKSADDVLGNPSKFVKKKLVPTIKKYLDGEVQRHCIIYGIEDNFDIKPIYHLKNDQITWMEKKANEELNEENVKIHILPIPYNNGLILAVYMIPIYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQY6</accession>
    <name>N6VQY6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_03109</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96315.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590772.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQY6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00168"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.610"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="144"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="247"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="281"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96315.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="317" mass="36966" checksum="BC1B3E3594EA2D74" modified="2013-06-26" version="1">MENQKILLQYYYLCKAWELFLENNYLEALYYVNKSLEINEDPLALLLKGIILFCEGKLKEAKEIFESSEKLKNNALILSLIAMIFGAEEGKCRMCIKILEENEDKVRNLIISLLIKSRCLEALGDYYKLLEVCDEILKRYPNFNAIKIKKADVLRKFGKYDEALRYVDEVLENNPNNVNALYLKGVILKRKGDYKSSLECFSRIIDDLKMEFLDAVRHAASVSLILGDLEKAERYINIGLKIRSDDIALWYFKGQLLERLGDEDGAIRCYSKVIELYPNHVKAYICLARIYERKGQLDKAINHYEKAFESKKKDFVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR55</accession>
    <name>N6VR55_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Fibrillarin-like rRNA/tRNA 2'-O-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">flpA</name>
        <name evidence="8" type="ORF">J422_06917</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in pre-rRNA and tRNA processing. Utilizes the methyl donor S-adenosyl-L-methionine to catalyze the site-specific 2'-hydroxyl methylation of ribose moieties in rRNA and tRNA. Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with nop5. Component of box C/D small ribonucleoprotein (sRNP) particles that contain rpl7ae, FlpA and nop5, plus a guide RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the methyltransferase superfamily. Fibrillarin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000064">
        <property type="protein sequence ID" value="ENN95611.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594595.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000064.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR55"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00932"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1341"/>
    <dbReference type="OrthoDB" id="6244at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990259">
        <property type="term" value="F:histone H2AQ104 methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008649">
        <property type="term" value="F:rRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000494">
        <property type="term" value="P:box C/D sno(s)RNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00351">
        <property type="entry name" value="RNA_methyltransf_FlpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000692">
        <property type="entry name" value="Fibrillarin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020813">
        <property type="entry name" value="Fibrillarin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003276">
        <property type="entry name" value="PRK04266.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003277">
        <property type="entry name" value="PRK04266.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003279">
        <property type="entry name" value="PRK04266.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335:SF17">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10335">
        <property type="entry name" value="RRNA 2-O-METHYLTRANSFERASE FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01269">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006540">
        <property type="entry name" value="Nop17p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00052">
        <property type="entry name" value="FIBRILLARIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM01206">
        <property type="entry name" value="Fibrillarin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00566">
        <property type="entry name" value="FIBRILLARIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0819">tRNA processing</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="81"/>
            <end position="82"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="99"/>
            <end position="100"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="124"/>
            <end position="125"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="143"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010632"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95611.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="222" mass="25632" checksum="BA5791096CE9F5FD" modified="2013-06-26" version="1">MIKEIFENVYKIEIDGEERLATKALSSKRVYDERFIKINNVEYRVWNPNKSKLAAAIIKGLKFLPIKKGSKVLYLGASAGTTPSHVSDIVENGIVYAVEYSPRIIRELLDVAEDRKNIIPILGDANKPQEYSYIEKVDVIYEDVAQPNQAEILIKNAKWFLKENGYAMIAIKARSIDVVREPKEIFKEQKEILENNGFKVLEEIDIEPFEKDHIMFLLEWRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRD9</accession>
    <name>N6VRD9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Methyl-coenzyme M reductase subunit alpha</fullName>
            <ecNumber evidence="11">2.8.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">J422_02185</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>coenzyme B + methyl-coenzyme M = methane + coenzyme M-coenzyme B heterodisulfide</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
        <text evidence="11">Binds 2 coenzyme F430 non-covalently per MCR complex. Coenzyme F430 is a yellow nickel porphinoid. Methyl-coenzyme-M reductase is activated when the enzyme-bound coenzyme F430 is reduced to the Ni(I) oxidation state.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the methyl-coenzyme M reductase alpha subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96460.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590263.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRD9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01185"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.429"/>
    <dbReference type="OrthoDB" id="52468at2157"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.840.10">
        <property type="entry name" value="Methyl-coenzyme M reductase, alpha/beta subunit, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.390.10">
        <property type="entry name" value="Methyl-coenzyme M Reductase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016212">
        <property type="entry name" value="Me_CoM_Rdtase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008924">
        <property type="entry name" value="Me_CoM_Rdtase_asu/bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009047">
        <property type="entry name" value="Me_CoM_Rdtase_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003183">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015811">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015823">
        <property type="entry name" value="Me_CoM_Rdtase_asu_N_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03256">
        <property type="entry name" value="met_CoM_red_alp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02249">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02745">
        <property type="entry name" value="MCR_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000262">
        <property type="entry name" value="MCR_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48081">
        <property type="entry name" value="Methyl-coenzyme M reductase alpha and beta chain C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="Methyl-coenzyme M reductase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="5" id="KW-0488">Methylation</keyword>
    <keyword evidence="6 11 12" id="KW-0533">Nickel</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyl-coenzyme M reductase alpha subunit N-terminal" evidence="15">
        <location>
            <begin position="5"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-coenzyme M reductase alpha subunit C-terminal" evidence="14">
        <location>
            <begin position="318"/>
            <end position="444"/>
        </location>
    </feature>
    <feature type="binding site" description="axial binding residue" evidence="12">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </ligand>
        <ligandPart>
            <name>Ni</name>
            <dbReference type="ChEBI" id="CHEBI:28112"/>
        </ligandPart>
    </feature>
    <feature type="modified residue" description="Pros-methylhistidine" evidence="13">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="modified residue" description="5-methylarginine" evidence="13">
        <location>
            <position position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRNR" id="PIRNR000262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR000262-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000262-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02745"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN96460.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="553" mass="61618" checksum="CB560C1CF164C9CB" modified="2013-06-26" version="1">MDAEKRLFLKALKEKFEEDPREKYTKFYIFGGWKQSKRKREFVEWAQKLVEKRGGIPFYNPDIGVPLGQRKLMPYKVSNTDAIVEGDDLHFMNNAAMQQMWDDIRRTVIVGLDTAHTVLEKRLGVEVTPETINEYMEVINHALPGGAVVQEHMVEVHPGLVWDCYAKIFTGDDELADEIDKRFLIDINKLFPEEQAEQIKKAIGKRTYQVSRVPTLVGRCCDGGTIARWSAMQIGMSFITAYKLCAGEAAIADFAYAAKHADVIQMANFLPARRARGPNEPGGIYFGVFADIIQTSRVSDDPVEQSLEVVGAGAMLFDQIWLGGYMSGGVGFTQYATASYTDDILDDFSYYGYDYIQKKYGGCNSVKPTMDVVEDIATEVTLYALEQYDEFPALLEDHFGGSQRAAVTAAAAGISVALATGNSNAGLNGWYLSQIIHKEYHSRLGFYGYDLQDQCGAANSLSFRNDEGSPLELRGPNYPNYAMNVGHQGEYAGITQAAHSARGDAFALNPLIKVAFADPSLIFDFRYPRKEFARGALREFEPAGERDIIIPAH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRN8</accession>
    <name>N6VRN8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH-ubiquinone oxidoreductase chain 49kDa</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_05778</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592818.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRN8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01250"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1126"/>
    <dbReference type="OrthoDB" id="43567at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052197">
        <property type="entry name" value="ComplexI_49kDa-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43485:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43485">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0533">Nickel</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="3">
        <location>
            <begin position="118"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="3">
        <location>
            <begin position="286"/>
            <end position="357"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601501-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="365" mass="41700" checksum="85E249B95537EC48" modified="2013-06-26" version="1">MSLPIGPIHPALKEPIRIKLILEGEKPVDAEIEMGYVHRGIENIMEGKHVNKAVFLAERVCGICSFVHTVTFSECVEKISNIEIPDKARYLRVILIELERIHSHLIAIASYCLSIEHETLAIWLLNVREKILDLIEMITGNRINMGYCIVGGVRRDINKEIINKINENLDDFEEKFKNILDVFETAPLVGLRSKGIGVLTYKEIMRTRAVGPVCRASGLPESDWRLRHTTYKELNFKPVWKNEGDNFARNLVRFYEIFESIKLIRKALELYEESSGDIRVKPEIKAGVGEHRFEAPRGELLYRMELTNGGLIKRVEIRTPTVMNLEAYKYMLKSCPTVADAVSCYTSIDPCISCTERAIIIKKVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRQ3</accession>
    <name>N6VRQ3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02035</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96550.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590187.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRQ3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00252"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.399"/>
    <dbReference type="OrthoDB" id="63782at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="94"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96550.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="152" mass="16241" checksum="2508A731851B987B" modified="2013-06-26" version="1">MRISPLVAGLIGGFSAALLQAFFKVSPPPAYGICIACHTRDLVNWIVNAIFGTTLPMAPVSKVFPVLTVVGIFIGAFIAANVHKEFKIKQTHNPIVGFVLGVLVVIFALLMGGCPLRETIRTAYGDVIALISLIAMFTGVIVASEVYLKRNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRT1</accession>
    <name>N6VRT1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 2">Argininosuccinate lyase</fullName>
            <shortName evidence="1">ASAL</shortName>
            <ecNumber evidence="1 2">4.3.2.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Arginosuccinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">argH</name>
        <name evidence="6" type="ORF">J422_05484</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2-(N(omega)-L-arginino)succinate = fumarate + L-arginine</text>
            <dbReference type="Rhea" id="RHEA:24020"/>
            <dbReference type="ChEBI" id="CHEBI:29806"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:57472"/>
            <dbReference type="EC" id="4.3.2.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the lyase 1 family. Argininosuccinate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.2.1" evidence="1 2"/>
    <dbReference type="EMBL" id="APMM01000041">
        <property type="protein sequence ID" value="ENN95871.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592557.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRT1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01405"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1071"/>
    <dbReference type="OrthoDB" id="27337at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00114"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004056">
        <property type="term" value="F:argininosuccinate lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01359">
        <property type="entry name" value="Argininosuccinate_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.40.30:FF:000001">
        <property type="entry name" value="Argininosuccinate lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.200.10:FF:000015">
        <property type="entry name" value="argininosuccinate lyase isoform X2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.40.30">
        <property type="entry name" value="Fumarase/aspartase (C-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.200.10">
        <property type="entry name" value="Fumarase/aspartase (Central domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.275.10">
        <property type="entry name" value="Fumarase/aspartase (N-terminal domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00006">
        <property type="entry name" value="Arg_succ_lyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029419">
        <property type="entry name" value="Arg_succ_lyase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009049">
        <property type="entry name" value="Argininosuccinate_lyase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024083">
        <property type="entry name" value="Fumarase/histidase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000362">
        <property type="entry name" value="Fumarate_lyase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022761">
        <property type="entry name" value="Fumarate_lyase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008948">
        <property type="entry name" value="L-Aspartase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00838">
        <property type="entry name" value="argH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43814:SF1">
        <property type="entry name" value="ARGININOSUCCINATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14698">
        <property type="entry name" value="ASL_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00206">
        <property type="entry name" value="Lyase_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00145">
        <property type="entry name" value="ARGSUCLYASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00149">
        <property type="entry name" value="FUMRATELYASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48557">
        <property type="entry name" value="L-aspartase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 6" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarate lyase N-terminal" evidence="4">
        <location>
            <begin position="17"/>
            <end position="299"/>
        </location>
    </feature>
    <feature type="domain" description="Argininosuccinate lyase C-terminal" evidence="5">
        <location>
            <begin position="362"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="402"/>
            <end position="476"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="NCBIfam" id="TIGR00838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF14698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95871.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="482" mass="55187" checksum="2D08F9AC654B576E" modified="2013-06-26" version="1">MNILRRGRLGEIKEDVARYTTSLSFDKEIFEADILCDIAHVIMLYKQDILKKEEAEKIISGLKEIYKDGLDKLNLDPSLDDIHMVIENELYKRIDDVAGKLQTARSRNDQVATDLRLALREKIVNILISLIAFLEDLTELSKEYKDVITVGYTHLQHAQPITFGHLLLSYVSAIERDILRLLDSYKRVNISPLGSCALAGTGFNIDREMTKELLGFDGIIENTIDAVSARDFIAEVVANLSILSANLSKICEELILFSTYEFNTVEIDDSYCSTSSIMPQKKNPDVAEIARAKLCSIYGNLITILTILKALPNAYNRDLQEITPYLWDSIYKITDTIKMVHGMLKSLKINKDRMYFLAKANYSTATELADTLVRELNIPFRKAHNIVGEIVRISIKERKDVIKTAKEVLKKYNLEIDEEKIEKALKPEENVKLRKTLGGPNPEEVEKRAESFKKRLEEYKRNVLMKKEKIENVKNMLLNYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRU6</accession>
    <name>N6VRU6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_01620</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96580.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589983.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRU6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00451"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.319"/>
    <dbReference type="OrthoDB" id="67071at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004911">
        <property type="entry name" value="PRK06267.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="BIOTIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976:SF2">
        <property type="entry name" value="BIOTIN SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="6">
        <location>
            <begin position="16"/>
            <end position="234"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96580.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="347" mass="39794" checksum="6E5D6B5D38DBD1C2" modified="2013-06-26" version="1">MIVDEILENVRKAYKITRRHFNYVSFERALFLGWYCNLKHPCKFCYMSTQKNKIKNPEKAKRSLSSILAEAILMKRIGWKLEFISSGYGFSEKEINNIAEMVAYVQKCKQFLNTGVINLDEINLDIIEGVTGAVETVVKNRDEICPGKPLERIKEFLLKAKERDLKTGITIILGLGEKEEDISKLLDLIEELDLDKVIFYSLNPQKGTIYENKPTVTTLEYMNWVSSVRLAFPKIKIATGVWVDKISMISPLICSGSNVITKFPVFSIYGTKYAYWVEKEIKTVAKLLGTFTDLEVLMGAKELKETPYIDEDIKINEKNKEKVEGLKGEIDKKINSYVNSVLKRVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRY7</accession>
    <name>N6VRY7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">tRNA (guanine(10)-N(2))-dimethyltransferase</fullName>
            <ecNumber evidence="13">2.1.1.213</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">tRNA:G10 dimethyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="17" type="ORF">J422_01061</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the adenosylmethionine-dependent methylation of the exocyclic amino group (N(2)) of guanosine at position 10 of various tRNAs. Acts via a two-step process that leads to the formation of either N(2)-monomethyl (m(2)G) or N(2)-dimethylguanosine (m(2)(2)G).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>guanosine(10) in tRNA + 2 S-adenosyl-L-methionine = N(2)-dimethylguanosine(10) in tRNA + 2 S-adenosyl-L-homocysteine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:43124"/>
            <dbReference type="Rhea" id="RHEA-COMP:10355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10358"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74269"/>
            <dbReference type="ChEBI" id="CHEBI:74513"/>
            <dbReference type="EC" id="2.1.1.213"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the methyltransferase superfamily. Trm-G10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.213" evidence="13"/>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589880.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRY7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00831"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.206"/>
    <dbReference type="OrthoDB" id="7080at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160101">
        <property type="term" value="F:tRNA (guanine(10)-N2)-dimethyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160102">
        <property type="term" value="F:tRNA (guanine(10)-N2)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11715">
        <property type="entry name" value="THUMP_AdoMetMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000251">
        <property type="entry name" value="Putative RNA methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053943">
        <property type="entry name" value="RlmKL-like_Mtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005885">
        <property type="entry name" value="TrmG10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01177">
        <property type="entry name" value="TIGR01177 family methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911:SF13">
        <property type="entry name" value="TRNA (GUANINE(6)-N2)-METHYLTRANSFERASE THUMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017259">
        <property type="entry name" value="tRNA_mtfrase_TRM11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01261">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 17" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 15" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="4" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="16">
        <location>
            <begin position="46"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00051883"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00054380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00061338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00066936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00082665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN96640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="335" mass="38523" checksum="F3C0989A8263124C" modified="2013-06-26" version="1">MIGYVLSGDHTDLAFAELNALLDIFGYKGKIERLDRYVLTGESRGEEIVNRAGYINESHKIIYSYNYENFSLEGFLRDIERSLEIKINGSYAVRILKLHKDKSINSQLLEREIGAIIKRKTGAKVNLTNPENLIRVVILKDRIFIGNLIKMRDKKYFYENRPHLRKYFHPGSILPKLARAMVNLARVREGDIVLDPFCGTGGFLIEAGLIGAKLLGCDIDWRMARGSLINLEDYNLLDKVIKIECLDAKYVKDFLKRNNIEKVDAIITDPPYGISTAKKGEIEEIIKILPEVLKENGYFVFAYNKKINLNLNLEGLFKIPIHKQLVRYIHVYKKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS11</accession>
    <name>N6VS11_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA polymerase B1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04870</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95946.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS11"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.954"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006133">
        <property type="entry name" value="DNA-dir_DNA_pol_B_exonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03104">
        <property type="entry name" value="DNA_pol_B_exo1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B exonuclease" evidence="1">
        <location>
            <begin position="106"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95946.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="264" mass="31218" checksum="3FB78DBAA8C0F1F6" modified="2013-06-26" version="1">MIDALIDATYKGNERAIIYLYLINTILKDKEFKPYFYVELNKDEIDEEDIENLKAFLLKKDLLKFVDNIEVVKKRILKKEKNVLKIISTYPKHVPKLRKVKEIAKEIYEHDIPFVKRYMIDNDIPPMTYFDFEKKKIVSNEIPKIKMVAFDIEVSAEGEPDYRKNPIIMASFWDGEGKVITYKDFNHENVIKVKDERELIIKIIETLGKYDIIFTYNGDNFDFPYLKKRAEVYGIKLKLGREGEEVRIKRAANGYQSYIPGSTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS35</accession>
    <name>N6VS35_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein uS9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps9p</name>
        <name evidence="4" type="synonym">rps9</name>
        <name evidence="7" type="ORF">J422_04970</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uS9 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95966.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592103.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS35"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00496"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.974"/>
    <dbReference type="OrthoDB" id="52677at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000462">
        <property type="term" value="P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000051">
        <property type="entry name" value="30S ribosomal protein S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00532_A">
        <property type="entry name" value="Ribosomal_uS9_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000754">
        <property type="entry name" value="Ribosomal_uS9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019958">
        <property type="entry name" value="Ribosomal_uS9_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020574">
        <property type="entry name" value="Ribosomal_uS9_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001749">
        <property type="entry name" value="PRK00474.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03627">
        <property type="entry name" value="uS9_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569:SF16">
        <property type="entry name" value="RIBOSOMAL PROTEIN S16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21569">
        <property type="entry name" value="RIBOSOMAL PROTEIN S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00380">
        <property type="entry name" value="Ribosomal_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00360">
        <property type="entry name" value="RIBOSOMAL_S9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="111"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="120"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005251"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95966.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="136" mass="15342" checksum="22CB22FBD9E85058" modified="2013-06-26" version="1">MEKIVITVGKRKRAIARAVARPGKGRIRINRIPIELIEPKYKRMKLMEPLLLAGEEVISQMDIDVTVRGGGVMGQMDAARTAIGKAIVEFTGSKELRDKFLAYDRTLLVSDARRTEPHKPSRSTKGPRAKRQKSYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS58</accession>
    <name>N6VS58_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">S-layer protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05070</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95986.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592145.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS58"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00475"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.994"/>
    <dbReference type="OrthoDB" id="148350at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022651">
        <property type="entry name" value="S_layer_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05124">
        <property type="entry name" value="S_layer_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-layer protein outer" evidence="1">
        <location>
            <begin position="307"/>
            <end position="379"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF05124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95986.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="394" mass="46332" checksum="7C04D827DEAB86E5" modified="2013-06-26" version="1">MKKVLLIFLILTSVFAMEPIIVANKSTVDYNNSLILMDNYYSSKKIIIENDTVKMISRNILYLPFKNSLDIRLKNDILRVKIERNNDDIIFKDIYYIIKLDKEIEINGEKYNVKNFGNFILLLGDGENVTTKGSFEFNNYRINIKLVSMDHKSLIVDIYKDGVAVEKNVKLNMGEMYYSKGIAVKYKNYSKNLFEFTVYKAIKIEKGKDYPLDKRFTVEDINNYIKLKFKNSFKNRLNLSNCYIYPINKTLFKAIVEYIDSYKKENISFENGFYIMGNKVYYKGKEVRSGEKFYFGSVDIINNDIFNMDRDVILVGGERVNSYVRELVRRGLLKDRITETNPGRFKGYIIKIRNKNHFIYVLAGSDRWGTKAAIEAFLNRYHGEDKLLVNWKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS80</accession>
    <name>N6VS80_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 15">tRNA-splicing ligase RtcB</fullName>
            <ecNumber evidence="15">6.5.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">rtcB</name>
        <name evidence="16" type="ORF">J422_00826</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Essential for tRNA splicing and maturation. Acts by directly joining spliced tRNA halves to mature-sized tRNAs. Joins RNA with 2',3'-cyclic-phosphate or 3'-phosphate ends to RNA with 5'-hydroxy ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP + H2O = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:68080"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + a 5'-end dephospho-ribonucleoside-RNA + GTP = a ribonucleotidyl-ribonucleotide-RNA + GMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:68076"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:17355"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:173118"/>
            <dbReference type="EC" id="6.5.1.8"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14 15">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="14 15">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 15">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 15">Belongs to the RtcB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.-" evidence="15"/>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96735.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589835.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS80"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00869"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.163"/>
    <dbReference type="OrthoDB" id="9887at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003972">
        <property type="term" value="F:RNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0170057">
        <property type="term" value="F:RNA ligase (GTP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1860.10:FF:000001">
        <property type="entry name" value="tRNA-splicing ligase RtcB homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1860.10">
        <property type="entry name" value="tRNA-splicing ligase RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001233">
        <property type="entry name" value="RtcB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036025">
        <property type="entry name" value="RtcB-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11118:SF1">
        <property type="entry name" value="RNA-SPLICING LIGASE RTCB HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01139">
        <property type="entry name" value="RtcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103365">
        <property type="entry name" value="Hypothetical protein PH1602"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01288">
        <property type="entry name" value="UPF0027"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 13" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 15" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 14 15" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 14 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="GMP-histidine intermediate" evidence="12">
        <location>
            <position position="400"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="199"/>
            <end position="203"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="325"/>
            <end position="326"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="325"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="374"/>
            <end position="377"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="381"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="400"/>
            <end position="403"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="476"/>
        </location>
        <ligand>
            <name>GMP</name>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011245"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045316"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR601233-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU371113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN96735.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="477" mass="53219" checksum="93EBDF9B48058C90" modified="2013-06-26" version="1">MLNKISEVVWELPKDYKSCMRVPGRIYLNEILLDELEKEVLEQIANVACLPGIYKYSIAMPDVHYGYGFPIGGVGAFDLREGVISQEVLDFDINCGVRLIRTNLKAEEVKPKLNELIKTLFKNVPSGLGSKGILKFSKGEMDEVLEEGANWAVKNGYGWEKDIEHLEEHGCMKDADASYVSDKAKERGRVQLGSLGSGNHFLEVQFVERIFDEEAAKVYGIFEGQVVIMVHCGSRGLGHQVCTDYLRVLEKASKKYNIQLPDRQLACAPFESEEGQSYYKAMCCSANYAWANRQMITHWVRESFESVFKIHAEDLEMDVVYDVAHNIAKIEEHNIDGKRVKVIVHRKGATRAFPPGHESVPKDYKSIGQPVLLPGDMGTASYLMKGTEKAMEETFGSTAHGAGRKLSRAKALKLWRGAEIKRKLLEKGIIAMSDSKAVLAEEAPEAYKNIDLVADTCHTAGISLKVARMRPLGVIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS98</accession>
    <name>N6VS98_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH hxlR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_04775</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96026.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592008.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS98"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00974"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.934"/>
    <dbReference type="OrthoDB" id="65466at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002577">
        <property type="entry name" value="HTH_HxlR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33204">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, MARR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33204:SF18">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01638">
        <property type="entry name" value="HxlR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51118">
        <property type="entry name" value="HTH_HXLR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH hxlR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96026.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="103" mass="11975" checksum="8A1DA56D250784C7" modified="2013-06-26" version="1">MPIFHVLGKKGTIEIIYKIKDGINTFTKLKNSLDMDGCGVSTRTLAERLNELEQENIIQKEGSKYYLTKKGYELIEIIEQVKEWENKWEESKIPKIVINMLKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSA3</accession>
    <name>N6VSA3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Dihydroorotase</fullName>
            <shortName evidence="4">DHOase</shortName>
            <ecNumber evidence="4">3.5.2.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">pyrC</name>
        <name evidence="6" type="ORF">J422_00521</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the reversible cyclization of carbamoyl aspartate to dihydroorotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(S)-dihydroorotate + H2O = N-carbamoyl-L-aspartate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24296"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="EC" id="3.5.2.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the metallo-dependent hydrolases superfamily. DHOase family. Class I DHOase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.2.3" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96755.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589786.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSA3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00056"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.100"/>
    <dbReference type="OrthoDB" id="50279at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00117"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004038">
        <property type="term" value="F:allantoinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004151">
        <property type="term" value="F:dihydroorotase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006145">
        <property type="term" value="P:purine nucleobase catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01318">
        <property type="entry name" value="DHOase_IIb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00220_A">
        <property type="entry name" value="PyrC_classI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004722">
        <property type="entry name" value="DHOase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050138">
        <property type="entry name" value="DHOase/Allantoinase_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002195">
        <property type="entry name" value="Dihydroorotase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00857">
        <property type="entry name" value="pyrC_multi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43668:SF2">
        <property type="entry name" value="ALLANTOINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00483">
        <property type="entry name" value="DIHYDROOROTASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 4" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="5">
        <location>
            <begin position="46"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="active site" evidence="4">
        <location>
            <position position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <begin position="57"/>
            <end position="59"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="216"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="283"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="287"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-carboxylysine" evidence="4">
        <location>
            <position position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00220"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96755.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="404" mass="46291" checksum="B6ED887BB10FB5BC" modified="2013-06-26" version="1">MILKNCKIVKEDRIVEADIIIDGEKIKKIGKCINENDKCIDLKDYFVFPGVIDAHVHLRWRSKKEGFYSGSLAAINGGVCFVIDMPNDNPPIINKELFYEKLKEAKKQLVNIFLNFGVTDENYKLTLREAKAYKIFMVKSVGELYIRNYSKLKEILREDKLFCIHAEHKDIIEENYKKFSLNDWSDHCKVRCKKAEIEAIKIILNNLSRAKVHFCHVSTKEGLEVLKDKPVTVEVTPHHLYLNCDMANELKGLGKVNPPLRDKEDNIALIKALNDRVDIIASDHAPHLLEEKMKDVKNCPSGIPGLETLIPMTLNLVNSGYISLYTAAKLLSYNPSRIFNIDNGIREGNYANLTIVDIKKEGKIDVDLFKSKAKFSPFEGYKVRGFPIYTIVNGKFYESYGYYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSR4</accession>
    <name>N6VSR4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_03418</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000021">
        <property type="protein sequence ID" value="ENN96236.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591061.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSR4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00941"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.669"/>
    <dbReference type="OrthoDB" id="132045at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004256">
        <property type="entry name" value="DUF234"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34704">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34704:SF1">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03008">
        <property type="entry name" value="DUF234"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="DUF234" evidence="2">
        <location>
            <begin position="298"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96236.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="452" mass="53974" checksum="09C515C2B675344F" modified="2013-06-26" version="1">MFINREEELKALSEKLNSNTFEFVVIYGRRRIGKTKLALKSVENREHIYYLAVEGDNLKHFKRYVSKVVPTIEYAKEDWEAYFNFLKDKIIIIDEFPNLIKENPNILSLFQRIVDVHLKDTKTKLIILGSSISMMGEKILSYKSPLYGRKTGVLKIRPLKFKHLKEFFPNATWKELVEIYGFADGIPYYLEKIKLPFWDYLEEEIKRVDSFLRYEVDFLMKYEFEEPTTYKKILEAISFGNHSLGEIKNYLGVKHSDITPYLKNLIETEFIEREIPITESPKTKRGRYYIKDNFIAFYFRYIFPNLSAIEEGIFEIGEIKNDYNQYLGFVFEKVAKEFLIELNKNNKLPFKFLNIGRWWKKGEEIDLIALNKNEKKALFVEVKWKNLKDRDIKKILNDLKRKSELVELGDYEKYYAIIGRRVENKEDKDCLVFDLDDFDKYFSTIKNPPFCD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSY0</accession>
    <name>N6VSY0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03064</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96306.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSY0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00160"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.601"/>
    <dbReference type="OrthoDB" id="65724at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96306.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="675" mass="80138" checksum="FA9590A6BF327BD3" modified="2013-06-26" version="1">MKIQFVKKPFNVIRVYNMEFMHYFNNGKYDKVAKEIKRNPELLQELKKELDKGDKESLRRGLLVLKRLDNEIVKNFLYYVFLGLDSGNRFISKEAEFIIKKLVGKEVVEEAIVESYKYIDDRVLYYLIEHIDNPLYKKIILKYTNCDSLDKAILKIIDRKVLEILSENIYKNKCIVDILLKIVDKLGDEEKKILKEHLSPIILGKWDKNTYKKTKKLFYKLNVNYNLSEDCIINLLKNYKREALNLIVRENIRLSDECYSKILKTLLYSDEELQFIGVKLISLMDDSKKKVDFLFKFLSHGNGKAKTAAINELKKIIKTNKELADYIENKAICNIKIMNMSVKISSLRLLKEYSKKEYLDFLINEHKKIKNKIYQLDREKFKGGFRHLLFIEEEIRKCKIALNIIESIIAEICLKNRIHYKDLKIAEKLGYEFYKTIEIIGSKDLSLIDFDEFLEDVKKYCLITHLTEIIKNKYKNLDEEKAKKVIEIVENLNIHGDIYDANKIIIYALLGKKDKLGEILKLANNYQTYLAFIFAVKHFLKNKLLSDEEIKLLIPKIAEMLYNKRLRKEALKFFEEFPSKIAFPILISENIDDAVDVITKIILTYPELIYSLKERLYSNRRMVLKVLYNLSKYKREMLEDFIYILIILYNSSTYEERKLIKDIIKNVKPEYIKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTA3</accession>
    <name>N6VTA3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_02335</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SsuE family. Isf subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96426.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTA3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00346"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.459"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051796">
        <property type="entry name" value="ISF_SsuE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43278:SF1">
        <property type="entry name" value="IRON-SULFUR FLAVOPROTEIN MJ1083"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43278">
        <property type="entry name" value="NAD(P)H-DEPENDENT FMN-CONTAINING OXIDOREDUCTASE YWQN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADPH-dependent FMN reductase-like" evidence="6">
        <location>
            <begin position="2"/>
            <end position="151"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96426.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="191" mass="20611" checksum="B2B5BA523179A0E6" modified="2013-06-26" version="1">MILGISGSPREGNTSFLVKEALKAAANEGFDTEFISLAKKEMNPCIACNVCKETGQCQIIDDVEDILNKMREADGIIIGSPVYFGGVSAQLKILMDRSRPLRYGFQLKNKVGGAIAVGASRNGGQETTIQQIHNFFLIHSMIIVGDAEPTAHYGGTGVGKDPEDCKNDDIGLETARNLGRKVAEVVKLIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTQ7</accession>
    <name>N6VTQ7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02040</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96551.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590189.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTQ7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00253"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.400"/>
    <dbReference type="OrthoDB" id="65937at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007272">
        <property type="entry name" value="Sulf_transp_TsuA/YedE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04143">
        <property type="entry name" value="Sulf_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="41"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="131"/>
            <end position="156"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96551.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="157" mass="16910" checksum="6CEE7FA54B3298D6" modified="2013-06-26" version="1">MEYLHAIGTFIFGILLGYLFQRARMCFIGGMRDFYLIRDTWLIKGLFGFFGGALLGYIIFSAMSKIPFFPWIAVKGFTPIPGDPLGKAGSLVGHLIVAIIGGFGVGFFSVIQGGCPLRNYVMAAEGNKTAIAYILGLAVGAVLFHTVVVPIVKAILL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTU9</accession>
    <name>N6VTU9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycoside-hydrolase family GH114 TIM-barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01450</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000010">
        <property type="protein sequence ID" value="ENN96586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589951.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTU9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01024"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.284"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017853">
        <property type="entry name" value="GH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004352">
        <property type="entry name" value="GH114_TIM-barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35882">
        <property type="entry name" value="PELA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35882:SF2">
        <property type="entry name" value="PELA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03537">
        <property type="entry name" value="Glyco_hydro_114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51445">
        <property type="entry name" value="(Trans)glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside-hydrolase family GH114 TIM-barrel" evidence="1">
        <location>
            <begin position="42"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF03537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="217" mass="25741" checksum="B1F524280340E2A1" modified="2013-06-26" version="1">MRKFLLTFLFLILFCGCLNNTKIADDKPNINNKLNINNFWVYYGSDNIEKLSKYDLVIIEPYNYKKEDIQKLKLLNPNIKVIAYLSIGEVSKDRPYFKECQKIIIGKNQNWNSYYINISSPIWQKIILNEVKKFKNMGFDGVFLDTVDSAIYTNQKNEVIELIKKIREENPDIIIIQNRGFEIIEKTAPYIDGVLFEDFTTHYDFKNKKAYYWKVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU28</accession>
    <name>N6VU28_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL21</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl21e</name>
        <name evidence="7" type="ORF">J422_00931</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL21 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589856.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU28"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00588"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.183"/>
    <dbReference type="OrthoDB" id="6295at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.70:FF:000001">
        <property type="entry name" value="60S ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.70">
        <property type="entry name" value="Ribosomal protein L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00369">
        <property type="entry name" value="Ribosomal_eL21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001147">
        <property type="entry name" value="Ribosomal_eL21"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022856">
        <property type="entry name" value="Ribosomal_eL21_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018259">
        <property type="entry name" value="Ribosomal_eL21_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036948">
        <property type="entry name" value="Ribosomal_eL21_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003303">
        <property type="entry name" value="PRK04306.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20981">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01157">
        <property type="entry name" value="Ribosomal_L21e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01171">
        <property type="entry name" value="RIBOSOMAL_L21E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="6">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="6">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008427"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="98" mass="11352" checksum="5F647843BFC95BA9" modified="2013-06-26" version="1">MVEMSEGFRRKTRKKLSKHPRERGLYPITRALKEYKEGEYVHIVIDPSVHKGMPHPRFHGRTGVVVGKQGRAFVVKVRDGGKYKYIIAYPQHLRPAQA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VUA5</accession>
    <name>N6VUA5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hydrogenase subunit F</fullName>
            <ecNumber evidence="8">1.6.5.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_00401</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.6.5.3" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589762.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VUA5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00700"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.77"/>
    <dbReference type="OrthoDB" id="371891at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050586">
        <property type="entry name" value="CPA3_Na-H_Antiporter_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001750">
        <property type="entry name" value="ND/Mrp_TM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004920">
        <property type="entry name" value="PRK06277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT D1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42703">
        <property type="entry name" value="NADH DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00361">
        <property type="entry name" value="Proton_antipo_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="73"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="100"/>
            <end position="119"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="155"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="195"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="228"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="262"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="314"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="349"/>
            <end position="370"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="397"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="437"/>
            <end position="455"/>
        </location>
    </feature>
    <feature type="domain" description="NADH:quinone oxidoreductase/Mrp antiporter transmembrane" evidence="7">
        <location>
            <begin position="119"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00361"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="471" mass="51170" checksum="27056EB4BEDB580D" modified="2013-06-26" version="1">MELLPLIVVFPLIMAIIMNILHGKDKVIEYLTYIIAISLIILPFLANYGYYYFGGHGVIDGLVSGIAYLYNPAKKIIIITLAIVASLILIAGMSEKQKNSMFVTLTLMGFASVSAIILSDDLFNLYVFFEIISIVQSGLTILSGTENAYKAALRYLIIGNAAGALMLLGIGILLANTGTLNITDMKSLLPENITIYMAMLLLTVGLAYGAGLPPFHNIKADLYGRAKGFISAILQTYSKFILVGILLVMLKLFNGLSIFENTHAILIALGVFAMVFGVVMALLQKDYKRLLAYHAISQGGYVATGIAIGTPLGIVAGVFHAINHVIYKSALFLGAYIVSHKKGSNLYKLGGLISVMPFIAFLILCAKLAISGVPPFNGYQSKLLLAQAAMQANYPEIAIIMIIVSIGTFVSMMKAFYLIFLKPNNVEELKDFKCPKLAVFSLTVLVALCIIIGIYPDIVLNYLWKVFGKVL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VUE2</accession>
    <name>N6VUE2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl30e</name>
        <name evidence="7" type="ORF">J422_00165</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589722.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VUE2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01135"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.33"/>
    <dbReference type="OrthoDB" id="10759at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000053">
        <property type="entry name" value="50S ribosomal protein L30e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00481">
        <property type="entry name" value="Ribosomal_eL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000231">
        <property type="entry name" value="Ribosomal_eL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039109">
        <property type="entry name" value="Ribosomal_eL30-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022991">
        <property type="entry name" value="Ribosomal_eL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004038">
        <property type="entry name" value="Ribosomal_eL8/eL30/eS12/Gad45"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002172">
        <property type="entry name" value="PRK01018.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11449">
        <property type="entry name" value="RIBOSOMAL PROTEIN L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01248">
        <property type="entry name" value="Ribosomal_L7Ae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00709">
        <property type="entry name" value="RIBOSOMAL_L30E_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00993">
        <property type="entry name" value="RIBOSOMAL_L30E_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein eL8/eL30/eS12/Gadd45" evidence="6">
        <location>
            <begin position="2"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="101" mass="11067" checksum="208B8E597C583A3B" modified="2013-06-26" version="1">MDVNKAIRTAVDTGKVILGSRRTLKYLKHGEGKLVIIAGNVPKELEEDVKYYAKLSNIPVYQHNLTSLELGAVCGKPFPVSTLLIIDEGLSNIMDVLKGDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VUG1</accession>
    <name>N6VUG1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Bifunctional IPC transferase and DIPP synthase</fullName>
            <ecNumber evidence="6">2.7.7.74</ecNumber>
            <ecNumber evidence="7">2.7.8.34</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">J422_00250</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>1D-myo-inositol 3-phosphate + CTP + H(+) = CDP-1L-myo-inositol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:30647"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:62573"/>
            <dbReference type="EC" id="2.7.7.74"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>CDP-1L-myo-inositol + 1D-myo-inositol 3-phosphate = bis(1L-myo-inositol) 3,1'-phosphate 1-phosphate + CMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31327"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58401"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:62573"/>
            <dbReference type="ChEBI" id="CHEBI:62576"/>
            <dbReference type="EC" id="2.7.8.34"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="17">Belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">In the C-terminal section; belongs to the CDP-alcohol phosphatidyltransferase class-I family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">In the N-terminal section; belongs to the MobA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.74" evidence="6"/>
    <dbReference type="EC" id="2.7.8.34" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589737.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VUG1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01119"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.50"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.1760:FF:000042">
        <property type="entry name" value="Bifunctional IPC transferase and DIPP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.550.10:FF:000282">
        <property type="entry name" value="Bifunctional IPC transferase and DIPP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000462">
        <property type="entry name" value="CDP-OH_P_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043130">
        <property type="entry name" value="CDP-OH_PTrfase_TM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048254">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053433">
        <property type="entry name" value="IPC_transferase/DIPP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041135">
        <property type="entry name" value="IPPtranDIPPsyn_Thcocales"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136:SF84">
        <property type="entry name" value="BIFUNCTIONAL IPC TRANSFERASE AND DIPP SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01066">
        <property type="entry name" value="CDP-OH_P_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00379">
        <property type="entry name" value="CDP_ALCOHOL_P_TRANSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14 18" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="10 18" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 18" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="255"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="333"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="18">
        <location>
            <begin position="381"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="domain" description="MobA-like NTP transferase" evidence="19">
        <location>
            <begin position="3"/>
            <end position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00007897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00018322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00049235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU003750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ENN96831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="419" mass="48163" checksum="D252B841B8F6199C" modified="2013-06-26" version="1">MEAVILSAGYGSRMGNIPKSLLKICGKEILYRTMKLLSECGVNKFYIVVNNIYRKDFEDFLKKHNFNAHLIINNNPERGNGYSLHIVKNHVKDRFILVMSDHIYTKEFIEDAIKGEGLIVDKYPKYVDIDEATKVKVKDGRVEKIGKELENFDAIDTGFFILDSGIFKITEELEKEKEIIEMREVVERAKLRVTFVNGKPWTDVDIPEDIKKAKRMLIETAVKGDKDGFISRYINRKISTKISYFLVDHFSPNTLTIWIFLFGILSALTVLYNPILGAILYQISSILDGVDGEIARARLETSKFGGYLDSILDRYVDGAFLSILAYKTLSSNLWFFIAMVALIGCIMISYTSERFRGAYGKDMYESIEILKKIPGKRDERIFFIMIMVILNLIKPMYLILAIYTNLRVAIMIYLSKKLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>N6VUH2</accession>
    <name>N6VUH2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Phosphoenolpyruvate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00300</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96841.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VUH2"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.60"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96841.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="48" mass="5639" checksum="26D3C4AE89544D65" modified="2013-06-26" version="1">MVRWGIDSVSANIDAVETIRRVVARTEQKVILDYIRDLHEEKTKNKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VUH7</accession>
    <name>N6VUH7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrogenase expression/formation protein HypE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_00325</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589752.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VUH7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01098"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.65"/>
    <dbReference type="OrthoDB" id="31494at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02197">
        <property type="entry name" value="HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011854">
        <property type="entry name" value="HypE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02124">
        <property type="entry name" value="hypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303:SF0">
        <property type="entry name" value="CARBAMOYL DEHYDRATASE HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30303">
        <property type="entry name" value="HYDROGENASE ISOENZYMES FORMATION PROTEIN HYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005644">
        <property type="entry name" value="Hdrgns_mtr_HypE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="2">
        <location>
            <begin position="39"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="3">
        <location>
            <begin position="159"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="329" mass="35426" checksum="02799FE022A10918" modified="2013-06-26" version="1">MITKMHGAGGRLMIEFIKNQILKNIELKKVNGLGLDDLDDAGTIKLDDKHIAFTTDSYTVKPIFFPGGDIGRLAVSGTVNDLSVVGAKPLALSLAFIIPEGYDEEKIERIIKSINKTSKEAEVAIIAGDTKVTDGLDEIIINTSGVGVAEKVIKDSGVKDGDAIIVSGNIAEHGLAILLSREEFEFETNIKSDVAPLNKMIENILDLEISAMKDPTRGGLAEALNELAEKSSVGITIFEDKIPISDEVSSVCEILGLDPLTIANEGKVVIAVDKKDAEECLERLREHPLGKNAEIIGYATKEHKGVLMETGIGKRIIDYPIGDPIPRVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXE5</accession>
    <name>N6VXE5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05873</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000045">
        <property type="protein sequence ID" value="ENN95797.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592896.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXE5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00849"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1145"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="126"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95797.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="146" mass="16963" checksum="ACE89C5C9621F53C" modified="2013-06-26" version="1">MKDDSSYLDFDFEKGKIREKIQEFLGNNTESKRILYEHIDSLEEFVKNTIKELKESEEGLSSEGKNKIWGAFLYYSTLVDVFTFSQLSKFMIDESEILRVEKLKAELEVAIALARSKYQAKSRRDIVRSIMYLSIALGIIFGLKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXG9</accession>
    <name>N6VXG9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05808</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592828.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXG9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01244"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1132"/>
    <dbReference type="OrthoDB" id="65851at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="840" mass="96817" checksum="86A1D92D4B03DCE2" modified="2013-06-26" version="1">MKVRLLLILLLTLTISYGEISIYKIWTPYDPNLVPIMYIDINEPVLYIGIVNKDEYAHNVVVKAELNGETFTTVPIYLPPNDHMEKIVELRIPVYDDKVHNVKVSLIENGKEIVSKNIPIKCYFPIDVKNVSCQDCCEYKDGEVCYSNWFYVVLKSNPLAQSDYNAKVWINVKSGNTILYDGKKDAKTVYIPWGNEVKLSFKIPKISLNNNDKFTIETYVVVMNVTHSVEGYEKTELKRDENGIYHKYNSYKKYFYFPVIIKNIDVYNKIDRDNVDLVKKFYSNVLDEELEEIINKRNYPIYGEIPRVYVKDETTLSFINVTLENRYNKDVIVKFIWEYGNNSYYKTIKINKNSDTSFILPIYSKPGNVNIKFSVNPIEVNSYIYNTSYTLNVNPKLIAPVIIKKIILPLDQSINEDVNGTVLVGKWYNITLIIKNKYYKPLSGYIEISDNFEKGVVNYTKRVEFNISAYGVKKIKIPIIFYREVNGDLKVTLKTKNGVKDDCRIIHFYAYLPIEVKEVKYNNSILHKISVVGNGGIYTYNPIAGYNNTCIVRLKNKLNKNISCKVWVEVYNKNGKLEAKSDIKFVKFKPDEEKTLKFNLYFNEGFEGYTLIHAVPMNLENVDVIFTQGLNYHLVQIPKYYEVGRYSHLSLLGLKVSTASHEITRVVSPISIKSLKFENNKIIAEIESNSFPTNLDVNYWINVNNYKSEKYSTNLPPKSSKVVEIPISLTSGNYTIEFCAEVDNFLSIDGKKCPILIKKYLNISILENKSSIASNQVKINGSYQNNITKINISSYKKDKNNKEIKNITNLTSKKSETSIIDAILSPIKVVIVKILEILPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXJ2</accession>
    <name>N6VXJ2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UDP-N-acetyl-D-mannosamine dehydrogenase</fullName>
            <ecNumber evidence="2">1.1.1.336</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">UDP-ManNAc 6-dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_05648</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>UDP-N-acetyl-alpha-D-mannosamine + 2 NAD(+) + H2O = UDP-N-acetyl-alpha-D-mannosaminouronate + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:25780"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:68623"/>
            <dbReference type="ChEBI" id="CHEBI:70731"/>
            <dbReference type="EC" id="1.1.1.336"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homotetramer; probably dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.336" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95852.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592700.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXJ2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00264"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1102"/>
    <dbReference type="OrthoDB" id="372050at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0089714">
        <property type="term" value="F:UDP-N-acetyl-D-mannosamine dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028359">
        <property type="entry name" value="UDP_ManNAc/GlcNAc_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43491">
        <property type="entry name" value="UDP-N-ACETYL-D-MANNOSAMINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43491:SF2">
        <property type="entry name" value="UDP-N-ACETYL-D-MANNOSAMINE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500136">
        <property type="entry name" value="UDP_ManNAc_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="UDP-glucose/GDP-mannose dehydrogenase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDP-glucose/GDP-mannose dehydrogenase C-terminal" evidence="10">
        <location>
            <begin position="293"/>
            <end position="389"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00034325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95852.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="408" mass="46246" checksum="6C40BE0AA9207999" modified="2013-06-26" version="1">MKICVIGLGYIGLPTASMLAIKGFKVVGVDINKSRVEEIKNLTFKTVEKDLDTLVKGAINSGNLIVKTEPEKADVFIICVPTPIDENKRCDLSYLNKAIEDIKPFLNEDNLIIIESTVPPGTCEELYRKLNKKVFLAYCPERVLPGNLLKELVENDRIIGGINKESAEMAKKIYESFVKGGIYLTDIRTAEMVKLMENIYRDVNIALANEFAKICDELSINVWEAINLANKHPRVNILKPGPGVGGHCISIDPWFIVEKSKHAKLIKTAREINDNMPLYVVEKIKKLKPRNIAIFGVAYKGNVDDVRESPAIKIVDLLLKNYNVKCYDSYVKSFTYPLYSLEEAVKDADLIVILADHDEYKNFSEEDIKIIKKLVRRHIIFDTKNVINKDLWEKEGFKVYLLGDGHIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXL2</accession>
    <name>N6VXL2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_05514</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000041">
        <property type="protein sequence ID" value="ENN95877.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592568.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXL2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00002"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1077"/>
    <dbReference type="OrthoDB" id="358785at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051198">
        <property type="entry name" value="BchE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023979">
        <property type="entry name" value="CHP04014_B12-bd/rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020612">
        <property type="entry name" value="Methylthiotransferase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04014">
        <property type="entry name" value="B12_SAM_MJ_0865"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409">
        <property type="entry name" value="ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409:SF17">
        <property type="entry name" value="METHYLTHIOTRANSFERASE MJ0865-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01217">
        <property type="entry name" value="B12-binding_methylthiotransfer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01278">
        <property type="entry name" value="MTTASE_RADICAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="150"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95877.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="428" mass="49085" checksum="490799C39F7554C6" modified="2013-06-26" version="1">MRVTIYSPHVYTYGAMLIGGILKNKYKVRLLRELDKTVFLKSDVVIFSLYSTLHILDKNIREAIDFLKRAKKKVYIAGCVSMYPEIILNELNVDGVIVGEGEITTPRILEGDKEGLAYKEGDEIVINYPKEKPDLNHPLPLIPKDIGKQSIRGANVYIETHRGCLGNCTFCQVPKFFGKQIRSREIEDIVREVKEFKKYGAKRIAISGGTGSLYGFKKSMEKEKFIELLEKISSILGKNNLSVPDMRVDYVDEDILKAIKDYTIGWVFYGIESGSDKILKDMKKGTNREKNLYAIQLAKEMGVKVAGSFIVAYPTESEKDYLLTKDFIAEAELDDIFVSIAEPIPKTEMCDLILDMNKEDLLLYKTYNHNLSVAEFRCYDLLVYGEMFKPNPRLSPYLYKIYLNEAKQQGEDIRKIIDIIFKYKDIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXM3</accession>
    <name>N6VXM3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 9 10">Diaminopimelate epimerase</fullName>
            <shortName evidence="9">DAP epimerase</shortName>
            <ecNumber evidence="3 9 10">5.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">PLP-independent amino acid racemase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9 12" type="primary">dapF</name>
        <name evidence="12" type="ORF">J422_05379</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the stereoinversion of LL-2,6-diaminopimelate (L,L-DAP) to meso-diaminopimelate (meso-DAP), a precursor of L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>(2S,6S)-2,6-diaminopimelate = meso-2,6-diaminopimelate</text>
            <dbReference type="Rhea" id="RHEA:15393"/>
            <dbReference type="ChEBI" id="CHEBI:57609"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="5.1.1.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the diaminopimelate epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.1.7" evidence="3 9 10"/>
    <dbReference type="EMBL" id="APMM01000038">
        <property type="protein sequence ID" value="ENN95887.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592442.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXM3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00462"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1050"/>
    <dbReference type="OrthoDB" id="358699at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00025"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008837">
        <property type="term" value="F:diaminopimelate epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.310.10:FF:000001">
        <property type="entry name" value="Diaminopimelate epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.10">
        <property type="entry name" value="Diaminopimelate Epimerase, Chain A, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00197">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018510">
        <property type="entry name" value="DAP_epimerase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001653">
        <property type="entry name" value="DAP_epimerase_DapF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00652">
        <property type="entry name" value="DapF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689:SF0">
        <property type="entry name" value="DIAMINOPIMELATE EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31689">
        <property type="entry name" value="DIAMINOPIMELATE EPIMERASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01678">
        <property type="entry name" value="DAP_epimerase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54506">
        <property type="entry name" value="Diaminopimelate epimerase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01326">
        <property type="entry name" value="DAP_EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6 9" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="11">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="74"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="9">
        <location>
            <position position="229"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="75"/>
            <end position="76"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="202"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="220"/>
            <end position="221"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="230"/>
            <end position="231"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="9">
        <location>
            <position position="165"/>
        </location>
    </feature>
    <feature type="site" description="Could be important to modulate the pK values of the two catalytic cysteine residues" evidence="9">
        <location>
            <position position="220"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051712"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00197"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR00652"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95887.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="286" mass="32546" checksum="0479F7ABE25DBF6E" modified="2013-06-26" version="1">MEFTKMQALGNDYIVIDEFNEEKIEESKKKNFVKKICLRKFYVGADGVIFIQKSDKYDVKFRIFNADGSEAEMCGNGIRCFAKYVYERILKKNPLYVETKAGLRICEIFGDNKADKIKVYMGKPSFKPEDIPISYNMEFINKTLKLKNPYIKEIKATAISVGNPHLIVFLEDNNLDEEFIRKHLNIIGEEIEHHNLFPERINVHFVKTINNNEIKIITWERGVGYTMACGTGATASAVVANKLGKTGKKVVAHLDGGKLEIEIKDDGVYIIGDAEMIFDGKLLVDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXN2</accession>
    <name>N6VXN2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tyrosine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tyrosyl-tRNA synthetase</fullName>
            <shortName evidence="8">TyrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">tyrS</name>
        <name evidence="10" type="ORF">J422_05314</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>tRNA(Tyr) + L-tyrosine + ATP = L-tyrosyl-tRNA(Tyr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10220"/>
            <dbReference type="Rhea" id="RHEA-COMP:9706"/>
            <dbReference type="Rhea" id="RHEA-COMP:9707"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78536"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the class-I aminoacyl-tRNA synthetase family. TyrS type 3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.1" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95897.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592376.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXN2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01324"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1037"/>
    <dbReference type="OrthoDB" id="8389at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004831">
        <property type="term" value="F:tyrosine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006437">
        <property type="term" value="P:tyrosyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02008">
        <property type="entry name" value="Tyr_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002307">
        <property type="entry name" value="Tyr-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023684">
        <property type="entry name" value="Tyr-tRNA-ligase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023617">
        <property type="entry name" value="Tyr-tRNA-ligase_arc/euk-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050489">
        <property type="entry name" value="Tyr-tRNA_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006330">
        <property type="entry name" value="PRK08560.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00234">
        <property type="entry name" value="tyrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264:SF4">
        <property type="entry name" value="TYROSINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46264">
        <property type="entry name" value="TYROSINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006588">
        <property type="entry name" value="TyrRS_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01040">
        <property type="entry name" value="TRNASYNTHTYR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="35"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="202"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>L-tyrosine</name>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048248"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95897.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="305" mass="35211" checksum="2F9DC8ED4415BC9A" modified="2013-06-26" version="1">MFERIKRNTVEIITEEELKKVLEKDEKKAYIGFEPSGKIHLGHYLQIRKMQDLKEAGFDIIILLADLHAYLNEKGDLEDIRKIGEYNKKVFKAMGLDAEYIYGSEFQLEKDYTLDVYKLALKTTLKRARRSMEMIAREEENPKVAEVIYPIMQVNDIKYLDVDVAVGGLEQRKIHMLARELLEKKPICIHNPVLTGLDGIGKMSSSKGNYIAVDDEPEVIKEKVKKAYCPIGVVENNPILEIAKYFLNYPLIIKRPEKFGGDIEVGSYVELEEKFKNKEIHPLDLKNTVANELISLLEPIRKRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXQ1</accession>
    <name>N6VXQ1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative molybdopterin-guanine dinucleotide biosynthesis protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_05194</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000034">
        <property type="protein sequence ID" value="ENN95917.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXQ1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00310"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02503">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00316">
        <property type="entry name" value="MobA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013482">
        <property type="entry name" value="Molybde_CF_guanTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136:SF81">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="8">
        <location>
            <begin position="7"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95917.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="195" mass="22316" checksum="AC1370052F482A53" modified="2013-06-26" version="1" fragment="single">MVCIIGAIVLAGGEGKRIGGEKPFKIFNGKCLLDYPVDLLMSLNIPYVIVFAKNPINTKKEREFIRKSYIVSFDLIENKGPLMGLLVGMRVLEKEWFLALPCDAPFITQEAIKKLINFIDNKYNIIIPKHKNGYIEPLFALYRRDCLKEIENIILEGKNLSLRNLIKRSKPLFIDAEIFGNIFININTLDDLNIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXR0</accession>
    <name>N6VXR0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit Rpo1C</fullName>
            <ecNumber evidence="8">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">DNA-directed RNA polymerase subunit A''</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpo1C</name>
        <name evidence="8" type="synonym">rpoA2</name>
        <name evidence="10" type="ORF">J422_05154</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Forms part of the jaw domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the RNA polymerase beta' chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000033">
        <property type="protein sequence ID" value="ENN95927.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592240.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXR0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01226"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1008"/>
    <dbReference type="OrthoDB" id="372142at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06528">
        <property type="entry name" value="RNAP_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.390">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00411">
        <property type="entry name" value="RNApol_arch_Rpo1C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045867">
        <property type="entry name" value="DNA-dir_RpoC_beta_prime"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007081">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012757">
        <property type="entry name" value="RPO1C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02389">
        <property type="entry name" value="RNA_pol_rpoA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19376:SF32">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE III SUBUNIT RPC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04998">
        <property type="entry name" value="RNA_pol_Rpb1_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="1 8 10" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase Rpb1" evidence="9">
        <location>
            <begin position="44"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF04998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95927.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="402" mass="45603" checksum="9937A40654B7E565" modified="2013-06-26" version="1">MDIETLKSKIYSLDLPESLKEELFEKLSKDNDLTDDMIEEIINEVVESYKKALIEPYEAVGIVAAQSIGEPGTQMTMRTFHYAGVAELNVTLGLPRMIEIVDARKEPSTPIMKIYLEKEYNNKEMAERIAKEIESLTLENVSENISIDLWNQAIKVELNEKMLEDRGLTVDEIVEIIRKKLKVKIDVDGYTLYLKIKSPSVKSLRKRMPKVKSIQLKGISGIKRVLVKKEDNEGYVLYTQGSNLREVLKIEGVDKRRTTTNNILEIQEVLGIEAARNAIIEEMKKTLEEQGLEVDIRHLMLVADIMTADGEVKPIGRHGVAGEKGSVLARAAFEETVKHLYSAAEKGEVDRLKGVIENVIVGKPIYVGTGCVELIIDREYEEGKVIDMDETERERKIYYAEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXU7</accession>
    <name>N6VXU7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">rps11p</name>
        <name evidence="7" type="synonym">rps11</name>
        <name evidence="9" type="ORF">J422_04950</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Located on the platform of the 30S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95962.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592095.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXU7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00500"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.970"/>
    <dbReference type="OrthoDB" id="12054at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.80:FF:000007">
        <property type="entry name" value="30S ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.80">
        <property type="entry name" value="Ribosomal protein S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01310">
        <property type="entry name" value="Ribosomal_uS11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001971">
        <property type="entry name" value="Ribosomal_uS11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019961">
        <property type="entry name" value="Ribosomal_uS11_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018102">
        <property type="entry name" value="Ribosomal_uS11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036967">
        <property type="entry name" value="Ribosomal_uS11_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03628">
        <property type="entry name" value="arch_S11P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007176">
        <property type="entry name" value="PRK09607.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11759">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S14/30S RIBOSOMAL PROTEIN S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00411">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002131">
        <property type="entry name" value="Ribosomal_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00054">
        <property type="entry name" value="RIBOSOMAL_S11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95962.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="129" mass="13989" checksum="10B21939403E430E" modified="2013-06-26" version="1">MAEQKKEIWAIVHIFSSYNNTIVHATDITGAETIARVSGGMITRNQRDEGSPYVAMQAAFRVAEILKERGIYNIHIKVRAPGGSGQKNPGPGAQAAIRALARAGLRIGRIEDVTPIPHDGTTPKKAYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXZ4</accession>
    <name>N6VXZ4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Signal transduction protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_04680</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96007.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591971.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXZ4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00954"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.915"/>
    <dbReference type="OrthoDB" id="8919at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051462">
        <property type="entry name" value="CBS_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108:SF6">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="7"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96007.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="118" mass="13653" checksum="8BF5A72C2323FD9F" modified="2013-06-26" version="1">MKVKDVMSKNFIKVSPNDVVGDVISLLYNERLYYAPVVEDDELVGWIDVLSILTSCKHSKVEDVMLYLYEIKVLNEDDEIDENFINEMINKRIIAYPVIKNNKLVGVLSIFDILKVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VY46</accession>
    <name>N6VY46_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF211 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04193</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96042.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591712.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VY46"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00683"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.820"/>
    <dbReference type="OrthoDB" id="201945at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1340">
        <property type="entry name" value="MTH889-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003831">
        <property type="entry name" value="DUF211"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023129">
        <property type="entry name" value="MTH889-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42240">
        <property type="entry name" value="DUF211 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42240:SF1">
        <property type="entry name" value="DUF211 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02680">
        <property type="entry name" value="DUF211"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160363">
        <property type="entry name" value="MTH889-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96042.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="97" mass="10951" checksum="A21BB3370840AB9B" modified="2013-06-26" version="1">MSGIRRLVLDILKPHEPKITDLALKLTAIPNVDGVNITVYEIDKETENVKITIEGKDLDFERIRDVIEMMGGTIHSIDEVVAGKKIIEEVKTPQDRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYN7</accession>
    <name>N6VYN7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0310 protein J422_03448</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_03448</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0310 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000021">
        <property type="protein sequence ID" value="ENN96242.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591072.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYN7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00935"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.674"/>
    <dbReference type="OrthoDB" id="35872at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd21132">
        <property type="entry name" value="EVE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.590.10">
        <property type="entry name" value="ph1033 like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00771">
        <property type="entry name" value="UPF0310"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002740">
        <property type="entry name" value="EVE_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022996">
        <property type="entry name" value="UPF0310"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002008">
        <property type="entry name" value="PRK00809.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39661">
        <property type="entry name" value="UPF0310 PROTEIN MJECL36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39661:SF1">
        <property type="entry name" value="UPF0310 PROTEIN MJECL36"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01878">
        <property type="entry name" value="EVE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="EVE" evidence="2">
        <location>
            <begin position="2"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96242.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="146" mass="17511" checksum="F746CAEEDF6BE89A" modified="2013-06-26" version="1">MAYWLCITNEDNWKIIKEKKIWGVAERHKNTINKVKVEDKLIIYEIQRSGKNYKPPYIRGVYEVVSEVYKDSTKVFKPTPRNPNEKFPWRVKLKEVKVFEPPINFKDLIPKLKFITNKKKWSGHLMGKAMREIPEEDYMLIVNKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ22</accession>
    <name>N6VZ22_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">dolichyl-phosphooligosaccharide-protein glycotransferase</fullName>
            <ecNumber evidence="6">2.4.99.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Oligosaccharyl transferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">J422_02844</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>an archaeal dolichyl phosphooligosaccharide + [protein]-L-asparagine = an archaeal dolichyl phosphate + a glycoprotein with the oligosaccharide chain attached by N-beta-D-glycosyl linkage to a protein L-asparagine.</text>
            <dbReference type="EC" id="2.4.99.21"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Protein modification; protein glycosylation.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cell membrane</location>
            <topology evidence="3">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the STT3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.99.21" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96377.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590634.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ22"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00118"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.558"/>
    <dbReference type="OrthoDB" id="82393at2157"/>
    <dbReference type="UniPathway" id="UPA00378"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004576">
        <property type="term" value="F:oligosaccharyl transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12610">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003674">
        <property type="entry name" value="Oligo_trans_STT3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048307">
        <property type="entry name" value="STT3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13872:SF1">
        <property type="entry name" value="DOLICHYL-DIPHOSPHOOLIGOSACCHARIDE--PROTEIN GLYCOSYLTRANSFERASE SUBUNIT STT3B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02516">
        <property type="entry name" value="STT3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="13 17" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 19" id="KW-0808">Transferase</keyword>
    <keyword evidence="9 17" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="12 17" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="137"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="164"/>
            <end position="181"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="225"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="259"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="284"/>
            <end position="301"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="307"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="345"/>
            <end position="366"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="411"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="445"/>
            <end position="462"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="468"/>
            <end position="485"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="497"/>
            <end position="518"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="530"/>
            <end position="551"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="17">
        <location>
            <begin position="563"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="domain" description="Oligosaccharyl transferase STT3 N-terminal" evidence="18">
        <location>
            <begin position="23"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00010810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00012602"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00030679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00034066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF02516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ENN96377.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="928" mass="105715" checksum="C5CA66F017F7845F" modified="2013-06-26" version="1">MIENISKFFNEKSWIKVLFILIVLMLLSFQLRAQTADMKFAGDNEFLKKLFSDEHGRMYLLALDPYYYLRLTENLYNHGHCGETLKEINGKLLPYDTCQYAPPGHPVSWEPPIICLTNLALYYIWHSIDNTVSIMNAAFWVPAVLSMLLPIPIFFIIRRVTNSYIGSAVGAIALITAPSLLYKTCAGFADTPIFEVLPILYIVWFILEALHYQDKTEIFNRNFKNIFSLFVIVSLIVEAILGAYLNISEGEITVFATEIFYMLSFMLLLFGIVIIIIKKIRNETIPFEIFLTLAIITTALAPKMWGGWWYGFDVIMIFFIIYLIAIKIFERYDFRFILTGNFKNVLYMTAFYIFGSLILLGIIYGANMILSPFLSPVGYQQVLSTYQLSTGWPNVYTTVAELEKPESWEEIFTASVGSPLLAIFGILGIIFSFISLRYGSLKIDIKYSLLLSLWLVVTLYASTKGIRFASLSTPPLVIGFGIFVGQLIRVLERKDILILYTLGSLSIIIGIIGIIKNYNKIYEIVVPSLYVPIAEYTLLIGLLVLFIYKLSDIYNSIRENRELIYKIASILLCLGIVIPQLSAVIPFSEPPTFNNGWKESLDWIKNNTPENAVITCWWDNGHIYTWATRKMVTFDGGSQNSPRAYWVGRAFATSNEELAVGILRMLATSGDEAFKKGSVLMNKTHNNVSKTVEILNEILPLNRSAAYKILTEKYGLTDKEAKEVLNATHPEHPNPDYLITYNRMTDIASVWSMFGFWNFSLPPNTSNDVREKGWFLKLRAKGIKINNSLDIVVILKKTVKYSIANIIEIKGENISTNNVLVDSTGRILKIEKPLIHRLIIYDKNNKKYYNKVINESANTSLIVRIDNNMVYAWLSSKNLEDSIYAKLHFLDGYGLKHIKLVKASIDPTSYGVQPGFKVYKVDYGNYLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ42</accession>
    <name>N6VZ42_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_02419</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">In the N-terminal section; belongs to the PINc/VapC protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96397.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590425.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ42"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00787"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.473"/>
    <dbReference type="OrthoDB" id="7146at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09878">
        <property type="entry name" value="PIN_VapC_VirB11L-ATPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052041">
        <property type="entry name" value="Nucleic_acid_metab_PIN/TRAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010335">
        <property type="entry name" value="PRK13764.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11603">
        <property type="entry name" value="AAA FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11603:SF147">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01850">
        <property type="entry name" value="PIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="PIN" evidence="4">
        <location>
            <begin position="22"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="3">
        <location>
            <begin position="503"/>
            <end position="566"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00046345"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96397.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="636" mass="72153" checksum="91E73C746D1C6172" modified="2013-06-26" version="1">MNLEERKKLEAKKIDELELIGKKVCVDTCVIIDGRISELIDKGKLSEATVIIPEAVISELEYQANMGREIGYQGIEELRKITEKAANYGIKIEYYGERPSKEAIELARSGEIDAIIRKVAKETGAILLTSDWIQYNLAVAQGIEAYYLETLEEEIELILNKYFDEETMSVHLKEGCLPYAKKGKPGNVKLVPIGDKELTKEDMEEIIDNIIKYAEQNNGFFEIQRKGATVIQLGNIRISIARPPFSDALEVTAVRPIIKAKLEDYGISDKLMERLKERAEGIFVSGPPGSGKSTFVAALAEFYRSQGKIVKTMESPRDLQVSKEITQYAPLEGDMEKTCDILLLVRPDYTIYDEVRKTRDFEIFADMRMAGVGMVGVVHASKPIDAIQRLIGRVELGVIPQVVDTVIFIKDGKIEKVYELDFTVKVPHGMFEEDLARPVIEVKDFETGKVEYEIYTYGEQVVVMPIKGEEVRKPIYSYAEEKLEEILKKLLPRKAKPIVKVTGDDSINLYVPERYIGSIIGKGGKEIAKLEDILGLRISVKEKEEEERKEMENIRRKYEYVTELESTKIYETDKHIIIDVGEDYAGENIRIYVDGKLLTTVTVRNDGTVRINKNTVVGKNLLKSIEEGRDIYIDLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZG5</accession>
    <name>N6VZG5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Thiamine biosynthesis protein-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01745</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96502.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590107.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZG5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00200"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.343"/>
    <dbReference type="OrthoDB" id="61764at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005232">
        <property type="entry name" value="LarE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052188">
        <property type="entry name" value="Ni-pincer_cofactor_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00268">
        <property type="entry name" value="ATP-dependent sacrificial sulfur transferase LarE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169">
        <property type="entry name" value="EXSB FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169:SF2">
        <property type="entry name" value="NAD_GMP SYNTHASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006661">
        <property type="entry name" value="PP-lp_UCP006661"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96502.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="242" mass="28611" checksum="9FB547A7F1419B57" modified="2013-06-26" version="1">MIKLDKYKGKKIIVAYSGGLDSLVLAILLNKVADILCVFIKTPYISNYSLNNAIYYAEKFNLKLNIIYVDKIIKNDENRCYYCKKMFFEILNNEKEKLGYDMVADGTNYDDIFEDRKGLKAKEELNIISPFVEAKVRKKDIIEYAKKLNIDIPKDSCLLMRFPINRDIKKEDLKRIEELEEFLRKFLFGVIRVRDFKDFAVIEVDDLNSIIKNREKIINKFKKYYKKVYLDLEGYSHKAPSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZL8</accession>
    <name>N6VZL8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">CoA-substrate-specific enzyme activase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_02020</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96547.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590181.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZL8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00249"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.396"/>
    <dbReference type="OrthoDB" id="114976at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008275">
        <property type="entry name" value="CoA_E_activase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051805">
        <property type="entry name" value="Dehydratase_Activator_Redct"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00241">
        <property type="entry name" value="CoA_E_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32329:SF2">
        <property type="entry name" value="BIFUNCTIONAL PROTEIN [INCLUDES 2-HYDROXYACYL-COA DEHYDRATASE (N-TER) AND ITS ACTIVATOR DOMAIN (C_TERM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32329">
        <property type="entry name" value="BIFUNCTIONAL PROTEIN [INCLUDES 2-HYDROXYACYL-COA DEHYDRATASE (N-TER) AND ITS ACTIVATOR DOMAIN (C_TERM)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase BadF/BadG/BcrA/BcrD type" evidence="5">
        <location>
            <begin position="3"/>
            <end position="241"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96547.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="244" mass="26881" checksum="D8A3B4894E620803" modified="2013-06-26" version="1">MILGIDVGSTTTKMVLMDNKKIVDYKIENIGVVVEEEDILKMVNEFEKEYNINKVVATGYGRHKITFADKIVPEVIALGRGANYFFREADGVVDIGGQDSKVIKIDKNGNVVDFILSDKCAAGTGKFLEKCIEILKIEDDINKYRSNNVAKISSMCAVFAESEIISLLAKKTPKEDILMGVYESICNRIVPMVNKLGISNIVFSGGVAKNRVLAEILKKKLNKSILIPKEPQIVCCVGAILKGF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZW4</accession>
    <name>N6VZW4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Transcriptional regulator NikR, CopG family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_01268</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the transcriptional regulatory CopG/NikR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589917.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZW4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00036"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.248"/>
    <dbReference type="OrthoDB" id="64466at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1220.10">
        <property type="entry name" value="Met repressor-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013321">
        <property type="entry name" value="Arc_rbn_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050192">
        <property type="entry name" value="CopG/NikR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014864">
        <property type="entry name" value="TF_NikR_Ni-bd_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719:SF2">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08753">
        <property type="entry name" value="NikR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="5">
        <location>
            <begin position="3"/>
            <end position="39"/>
        </location>
    </feature>
    <feature type="domain" description="Transcription factor NikR nickel binding C-terminal" evidence="6">
        <location>
            <begin position="50"/>
            <end position="121"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="124" mass="14568" checksum="907271460983A46C" modified="2013-06-26" version="1">MGRISITIEKNLLKEIDKINGENRSKVIREAVEYYIKKYDWLRRIESKLGEVSLIYNPKGKKDIIELESKYKDIIIVSLETRFNNKILRITAIKGDREKIIEFVNKLKGLKNVEMVKLTTISIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6W036</accession>
    <name>N6W036_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Molybdate/tungstate import ATP-binding protein WtpC</fullName>
            <ecNumber evidence="8">7.3.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">J422_00716</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Part of the ABC transporter complex WtpABC involved in molybdate/tungstate import. Responsible for energy coupling to the transport system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tungstate(in) + ATP + H2O = tungstate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35027"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.6" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589817.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W036"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00893"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.138"/>
    <dbReference type="OrthoDB" id="18368at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901238">
        <property type="term" value="F:ABC-type tungstate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03299">
        <property type="entry name" value="ABC_ModC_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000425">
        <property type="entry name" value="Probable ABC transporter, ATP-binding subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050093">
        <property type="entry name" value="ABC_SmlMolc_Importer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008995">
        <property type="entry name" value="Mo/tungstate-bd_C_term_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013611">
        <property type="entry name" value="Transp-assoc_OB_typ2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781:SF7">
        <property type="entry name" value="MOLYBDENUM ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781">
        <property type="entry name" value="SPERMIDINE/PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08402">
        <property type="entry name" value="TOBE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50331">
        <property type="entry name" value="MOP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="12">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00057369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="354" mass="39934" checksum="EBEA29F9F9597F60" modified="2013-06-26" version="1">MSFIQLENVNIKLGEFHLKDCSLSVEEGDYFTIIGPTGSGKTILLETIAGFYKPDSGRIIMNGIDITELPPEKRNISIVYQDCMLFPNMNVFDNIAYGLRKKIKDKETIKNEVIHIAKILDIEHLLDRYPTTLSGGEIQRTAIARALIVKPKLLLMDEPFSALDVKTKEKLRNLLKKVIDEYNTTVIHVTHDLDDVWSLANKVAVMRYGKILQIGTPEEIFNKPLPNFVADFVGTNVLTAEVIGKEGNLTVLNVNGLTIYSTDKLDLNDKLIKISIRPEKIIILNGHNNPNKKNIFTGKVKQVIKKGYFICLVVDINGININITITPNTHLLDFKEGDEIDILIKPEDVHIIKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6W041</accession>
    <name>N6W041_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Diaminopimelate aminotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_00741</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase M20A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589822.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W041"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00888"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.143"/>
    <dbReference type="OrthoDB" id="24854at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.10">
        <property type="entry name" value="Zn peptidases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010182">
        <property type="entry name" value="ArgE/DapE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036264">
        <property type="entry name" value="Bact_exopeptidase_dim_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002933">
        <property type="entry name" value="Peptidase_M20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011650">
        <property type="entry name" value="Peptidase_M20_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050072">
        <property type="entry name" value="Peptidase_M20A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01910">
        <property type="entry name" value="DapE-ArgE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010589">
        <property type="entry name" value="PRK13983.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808">
        <property type="entry name" value="ACETYLORNITHINE DEACETYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43808:SF32">
        <property type="entry name" value="ARGE_DAPE-RELATED DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07687">
        <property type="entry name" value="M20_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01546">
        <property type="entry name" value="Peptidase_M20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55031">
        <property type="entry name" value="Bacterial exopeptidase dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53187">
        <property type="entry name" value="Zn-dependent exopeptidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="7" id="KW-0170">Cobalt</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Peptidase M20 dimerisation" evidence="8">
        <location>
            <begin position="192"/>
            <end position="299"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006247"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF07687"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="405" mass="46410" checksum="8DCB25666E87A5B5" modified="2013-06-26" version="1">MEELAIKIASDLIKINSVNPAFGGNGEKEKAEYVKKIINDFIKIYNIHNYSLKEYNVKDKNGFERPNIVFKINFNREKSLHIISHLDTVPEGDIKLWETPPYEPVIKDGKIYGRGAEDNHKAIVSSLLLIPLIYESGLNPKYNLSLIFVSDEEAGSEYGLKYLLNFENEIFKKDDIIIVPDFSSKNGELIEIGEKGILWVLFSFIGSQCHGSTPEEGLNSNLIAFNFSNLLYNKLYNKYNKRDEIFLPPYSTFEPTIVKNNVTNPNTIPGYTEVAFDCRILPCYKIDDILEDIKKAINEFDFKKYINYFSGEPKIEYKILQRQEPNYTDKQSETIEELKRAIKNVLHVEPKLCGMGGGTVAAFLRYKGYNVAVWGIGEGTAHQPNEHIKIEDLIKMAKVFYEILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6W054</accession>
    <name>N6W054_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00836</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589837.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W054"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00867"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.165"/>
    <dbReference type="OrthoDB" id="64909at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="236" mass="27372" checksum="BD7268F2FE157BC5" modified="2013-06-26" version="1">MRYEIKIDEEKVEIVRYSTVYTDEGIEEIEETYLLTTADDYESVLGKYEPFPKKDVTYLGNLEDLKLVRGTELRTSVPIPLSICRPKYLRRVEEDDERITYLEINSAPIQRGIIVGIVVGVQHGKTSSGKDYTIFRVFDGYGWGKLRLFGIKANPELYTGLFIRGFVRFGLIEFRDEKGELRKAISLTLNDLPVLVQPKEYIVHKKFVDSVILKGIKEESQDNEDYEMENEEIIVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6W085</accession>
    <name>N6W085_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Monovalent cation/H+ antiporter subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_00406</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589763.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W085"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00699"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.78"/>
    <dbReference type="OrthoDB" id="18006at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.3510">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050601">
        <property type="entry name" value="CPA3_antiporter_subunitC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039428">
        <property type="entry name" value="NUOK/Mnh_C1-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005618">
        <property type="entry name" value="PRK07375.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34583">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34583:SF2">
        <property type="entry name" value="ANTIPORTER SUBUNIT MNHC2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00420">
        <property type="entry name" value="Oxidored_q2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="37"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="75"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="117" mass="12857" checksum="18CC22A88ACE8A5D" modified="2013-06-26" version="1">MDLQIASFIVSGLLVIIGLYGVFFIDNVLKKIIALEFLGNGVNLFLIAIGYNKIIPIKLPGISNEFFIKNSAYPLTHALVLTNIVIEASMLAVMLGIAIILYKKYKTLKGSVILKED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6W094</accession>
    <name>N6W094_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">ABC transporter releated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_00456</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589773.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W094"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00689"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.88"/>
    <dbReference type="OrthoDB" id="97750at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015807">
        <property type="term" value="P:L-amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03224">
        <property type="entry name" value="ABC_TM1139_LivF_branched"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052156">
        <property type="entry name" value="BCAA_Transport_ATP-bd_LivF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820:SF7">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43820">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="6">
        <location>
            <begin position="1"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="236" mass="26278" checksum="20F9E31FCAC1B26B" modified="2013-06-26" version="1">MIVKNLNAGYGKLQILFDVNAEIEKGKITTVVGPNGSGKSTFLKTLFGLTTIYSGEIIYNDVNITHLPPYKKARMGIAYLPQTNNVFQNLTVEENLKIAGYVLDKEKLKERIDLALEIFPEIKGILKRKAGSLSGGQRQFLAMAMALVRDAEVLMLDEPTAQLSPKLADLIFNKITEMRDELKLTILLVEQNAKKALEISDYGYMFVSGKVAFKGKAEDLLNHEKFKEYSLGVTAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="56" xmlns="https://uniprot.org/uniprot">
    <accession>N6W0B3</accession>
    <name>N6W0B3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Elongation factor 2</fullName>
            <shortName evidence="10">EF-2</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">fusA</name>
        <name evidence="12" type="ORF">J422_00145</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-G/EF-2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96812.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589718.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W0B3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01139"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.29"/>
    <dbReference type="OrthoDB" id="6290at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01681">
        <property type="entry name" value="aeEF2_snRNP_like_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01885">
        <property type="entry name" value="EF2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16268">
        <property type="entry name" value="EF2_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16261">
        <property type="entry name" value="EF2_snRNP_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01514">
        <property type="entry name" value="Elongation_Factor_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.240:FF:000010">
        <property type="entry name" value="Elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000684">
        <property type="entry name" value="Elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.870:FF:000002">
        <property type="entry name" value="Translation elongation factor 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.870">
        <property type="entry name" value="Elongation Factor G (Translational Gtpase), domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00054_A">
        <property type="entry name" value="EF_G_EF_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053905">
        <property type="entry name" value="EF-G-like_DII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041095">
        <property type="entry name" value="EFG_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000640">
        <property type="entry name" value="EFG_V-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004543">
        <property type="entry name" value="Transl_elong_EFG/EF2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005517">
        <property type="entry name" value="Transl_elong_EFG/EF2_IV"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00490">
        <property type="entry name" value="aEF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908:SF3">
        <property type="entry name" value="ELONGATION FACTOR-LIKE GTPASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42908">
        <property type="entry name" value="TRANSLATION ELONGATION FACTOR-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22042">
        <property type="entry name" value="EF-G_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00679">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14492">
        <property type="entry name" value="EFG_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03764">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SMART" id="SM00838">
        <property type="entry name" value="EFG_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00889">
        <property type="entry name" value="EFG_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10 12" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="8 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="11">
        <location>
            <begin position="19"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="28"/>
            <end position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="148"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Diphthamide" evidence="10">
        <location>
            <position position="602"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005870"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017891"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024731"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96812.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="726" mass="81161" checksum="441ECEBA67465989" modified="2013-06-26" version="1">MGKRAKMIAKIKELMEKYDRIRNIGICAHIDHGKTTLSDNLLAGAGMISKELAGEQLALDFDEEEAKRGITIFAANVSMVHNYEGKDYLINLIDTPGHVDFGGDVTRAMRAIDGAIVVVCAVEGVMPQTETVLRQALRERVKPVLFINKVDRLINELKLTPEELQARFIKIITDVNNLIKKMAPEEFKDKWLVKVEDGSVAFGSAYHKWAISVPFMKKSGITFKDIIEYCNQDRQRELAEKAPLHEVVLDMVIKHLPSPSEAQKYRIPHLWKGDINSDVGKAMLNCDPNGPLAGVVTKIIMDKHAGAVSVCRLFSGRIKQGMEVYMVNAQQKAKVQQVAVFMGPERIPVDSIAAGNICAIVGLKEASAGETICSPDKIIEPFEAIKHISEPVITVAIEAKNTKDLPKLIEVLRQIAREDPTVRVEINEETGEHLLSGMGELHIEIITKLKIERDAGIPVEVGQPIVVYRETVTKPSPIIESKSPNKHNKIYLMVEPLEEGVLQAYKEGRLSNIDFKKKPDDKTVEELIKAGMDPEEARRVISIYEGNVLVNMTRGIVHFDEVRELLIQGFKEAMNNGPLAAEKCQGVKVKIMDLVLHEDAIHRGPAQMIPAMRFGIREAMMQAEPVLLEPMQMVYISTPQDYMGAAMREISNRRGQILDMEQEGDMAIIKAKCPVAEMFGFAGAIRGATQGRCLWSLEFAGYERVPREMQDQLIRQIRERKGLKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTF5</accession>
    <name>N6UTF5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">J422_07017</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.</text>
            <dbReference type="EC" id="5.6.2.4"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the HerA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000068">
        <property type="protein sequence ID" value="ENN95584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594737.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTF5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00921"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1359"/>
    <dbReference type="OrthoDB" id="107033at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043138">
        <property type="term" value="F:3'-5' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043139">
        <property type="term" value="F:5'-3' DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008571">
        <property type="entry name" value="HerA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033186">
        <property type="entry name" value="HerA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002789">
        <property type="entry name" value="HerA_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42957:SF1">
        <property type="entry name" value="HELICASE MJ1565-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01935">
        <property type="entry name" value="DUF87"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05872">
        <property type="entry name" value="HerA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4" id="KW-0347">Helicase</keyword>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase HerA central" evidence="11">
        <location>
            <begin position="99"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase HerA-like C-terminal" evidence="12">
        <location>
            <begin position="324"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="13">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00034617"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF05872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN95584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="472" mass="54269" checksum="947B6C085B49B8B1" modified="2013-06-26" version="1" fragment="single">KKTPEVGDYVKIVYDGYELLGMIESTIQGNLALKDIWDVEHLEKIKEFGDEANYIMGKIKILGDINNDLKLPRVPPKPGLAIYKADNSILKRVFANGHIKIGKLITRDDVDIYLDVNKLCSRHLAILAMTGMGKSNTVAVLLKELNKLNATVLVFDVHGEYRNLECYEFKMDKYIISPYVNIYQISEDELCDLADVDPKATKQRAYIKRAIKEIKEENSENDFSDAKDYIKAIIERLEDYRSSEEYKRDEGSLLTAIFRLENFLNFKKKIITLHYNPVNHIREHCINIMPMEELDENDIDIIISYVAKQVLEDRKRIIIEKGRDFAKPIFLIFEEAHLIIPSNRETRAKYYLSRIAREGRKFGVGLCLVSQRPKTLDPETLSQCSNLIISKLIEPHDQKHVQMASESLSEDLMKHLTSLNIGEAIILGPCIKIPALVKIDKFNGEYGGEDLDLVKLWKKEEINDDIDNDAFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTG1</accession>
    <name>N6UTG1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cobalt ABC transporter, inner membrane subunit CbiQ</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_07007</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000067">
        <property type="protein sequence ID" value="ENN95594.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594703.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTG1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00576"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1357"/>
    <dbReference type="OrthoDB" id="51610at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16914">
        <property type="entry name" value="EcfT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012809">
        <property type="entry name" value="ECF_CbiQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051611">
        <property type="entry name" value="ECF_transporter_component"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02454">
        <property type="entry name" value="ECF_T_CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34857">
        <property type="entry name" value="SLL0384 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34857:SF2">
        <property type="entry name" value="SLL0384 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="126"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="254"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95594.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="276" mass="32175" checksum="2370E97D9DF54B1A" modified="2013-06-26" version="1">MNKLFDKSIEHMITYINEAIFHEKYARVPGVLQNIEPRIKIISLLTLIFFTLFTKNLYILVILNIFAILLAKLSKIPILTYIKRVYIFIPIFAGLIAFPVIFNIVTPGKDLFIILNSPHISITFEGVMYFITFIIRISTCISFAVLIPLTTKWNVILSTLRMFKVPEEIVTIFNLAYRYIFLLLNLALDILYSRKSRTAKKLSLLESWKEGGKIIGCLFINTCLVGEELYYSMLSRGFKEVTSFYDYKIKFKDIIFLIFVLAFILILKVLIWRFLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTK9</accession>
    <name>N6UTK9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methylated-DNA--protein-cysteine methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_06628</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000056">
        <property type="protein sequence ID" value="ENN95664.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593806.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTK9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00365"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1290"/>
    <dbReference type="OrthoDB" id="372118at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06445">
        <property type="entry name" value="ATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.460">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014048">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036217">
        <property type="entry name" value="MethylDNA_cys_MeTrfase_DNAb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055028">
        <property type="entry name" value="OGT_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00589">
        <property type="entry name" value="ogt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10815">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10815:SF13">
        <property type="entry name" value="METHYLATED-DNA--PROTEIN-CYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01035">
        <property type="entry name" value="DNA_binding_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22413">
        <property type="entry name" value="OGT_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46767">
        <property type="entry name" value="Methylated DNA-protein cysteine methyltransferase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0227">DNA damage</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methylated DNA-protein cysteine MeTransferase OGT N-terminal" evidence="3">
        <location>
            <begin position="2"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Methylated-DNA-[protein]-cysteine S-methyltransferase DNA binding" evidence="2">
        <location>
            <begin position="72"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95664.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="154" mass="17455" checksum="3AB8F8BFC2B09A4D" modified="2013-06-26" version="1">MIVEIDGYFIGLKFKDDYLVANTIPLKSKPKGFGELSDEYLDIAEIILKLYFAKMSDKEARKKVKHKLLVSPFIKKVLIFITNIPMGKTVTYGDVAKALNTSPRAVGRALNKNPLPLLYPCHRVVAKDSLGGYAYGLEEKKVILDREIKNFKYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTM1</accession>
    <name>N6UTM1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Glycosyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06513</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000053">
        <property type="protein sequence ID" value="ENN95679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTM1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00995"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1267"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="152" mass="17734" checksum="835D1BA1AEBCC7D8" modified="2013-06-26" version="1" fragment="single">KNKDVDIFYTNVQKYDITLRKKVKDKLADRLTILEDTTAKGFLEYSKKLPPAGGYISNLIFKRKAWNSVPNKNKYIGTAYVHFYIFITLLNRGSKIKFIPYKLVGQRLENDSFIREVGGKYNRLILDFNTLDIFKEVFGNTLGNMRSSKKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTN6</accession>
    <name>N6UTN6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Archaeal glutamate synthase [NADPH]</fullName>
            <ecNumber evidence="6">1.4.1.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_06411</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>2 L-glutamate + NADP(+) = L-glutamine + 2-oxoglutarate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15501"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="1.4.1.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the glutamate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.13" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593531.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTN6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01292"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1248"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004355">
        <property type="term" value="F:glutamate synthase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006537">
        <property type="term" value="P:glutamate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02808">
        <property type="entry name" value="GltS_FMN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024188">
        <property type="entry name" value="GltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043578">
        <property type="entry name" value="GltB_archl_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002932">
        <property type="entry name" value="Glu_synthdom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43819">
        <property type="entry name" value="ARCHAEAL-TYPE GLUTAMATE SYNTHASE [NADPH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43819:SF1">
        <property type="entry name" value="ARCHAEAL-TYPE GLUTAMATE SYNTHASE [NADPH]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01645">
        <property type="entry name" value="Glu_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500061">
        <property type="entry name" value="GOGAT_lg2_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006429">
        <property type="entry name" value="GOGAT_lg_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6" id="KW-0288">FMN</keyword>
    <keyword evidence="4 6" id="KW-0314">Glutamate biosynthesis</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0521">NADP</keyword>
    <keyword evidence="3 6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="10"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="39"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006429"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006429-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="503" mass="55161" checksum="D1ED676D986392BA" modified="2013-06-26" version="1">MIPSYVPPRYKVEVDHNRCMLCERCTIECSWGVFRREGDRIITYSNRCGACHRCITMCPRDAIRITKATEWRNHPVWDIEARTDIYNQAKTGCILLSGMGNALEHPRYFDHLVLDACQVTNPSIDPLREPMELRTYIGKKPKQLEFEFDEKIKKVKLKTKIAPNLKLDTPIMIAHMSYGALSLNAHLAFAKAVKECGTYMGSGEGGLPKELYPYADHVIVQVASGRFGVNPDYLERGAAIEIKIGQGAKPGIGGHLPGEKVTENISKTRMIPEGTDAISPAPHHDIYSIEDLAQLVRSLKEATEWKKPVFVKIAAVHNAPAIAVGIATSDADAVVIDGYRGGTGAAPKVFRDHVGIPLEMAIAKVDERLREEGLRNEISIIASGGIRNSADVFKAIALGADAVYIGTAAMVALGCRVCGRCYTGLCAWGIATQRPELVKRLDPEVGARRVANLIKAWTHELKELLGANGINSIESLRGNRDRLRGVGLNEVELRVLGVKPAGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTP1</accession>
    <name>N6UTP1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Potassium channel protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_06446</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593541.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTP1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01286"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1255"/>
    <dbReference type="OrthoDB" id="43518at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="entry name" value="Regulator of K+ conductance, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013099">
        <property type="entry name" value="K_chnl_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050721">
        <property type="entry name" value="Trk_Ktr_HKT_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833:SF13">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07885">
        <property type="entry name" value="Ion_trans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81324">
        <property type="entry name" value="Voltage-gated potassium channels"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="5" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="64"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="RCK N-terminal" evidence="3">
        <location>
            <begin position="107"/>
            <end position="226"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="4">
        <location>
            <begin position="252"/>
            <end position="337"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="344" mass="38876" checksum="F4C9B28FA9C3F073" modified="2013-06-26" version="1">MKARNKIIIVAILSMLLILTYAYLISVIENVDYFTALYFSVITITTTGYGDFVPKTTLGKAITVIYLCLGVGIVMYLFSLIAEYIAEGKFGEYMRMKIMENKIKKLKDHYIVCGYGRLGRVVGSYLIKENIPFVAIDVREDVLKEEAEKYDNFLYIVGDAKKNETLKKANIENAKCLIACLPTDADNVFLTLTAKELKSDILITAKANEFESIKKLKIAGANKVVSPYLIGGLRMAVLSTKRGVLDFLETFIEVAKGEYGEDINLKKFLIKKDSELSYKTLRDADIRKKTGATILGIKRKDQFFINPFPDFILKPGDVIYAFGTEESIKNLERLLEGKYDRKDN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU34</accession>
    <name>N6UU34_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DNA-binding protein J422_05564</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_05564</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the PDCD5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000042">
        <property type="protein sequence ID" value="ENN95864.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592617.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU34"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00754"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1086"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.140">
        <property type="entry name" value="PDCD5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00026">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022889">
        <property type="entry name" value="DNA_bind_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002836">
        <property type="entry name" value="PDCD5-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036883">
        <property type="entry name" value="PDCD5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003268">
        <property type="entry name" value="PRK04239.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10840:SF0">
        <property type="entry name" value="PROGRAMMED CELL DEATH PROTEIN 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01984">
        <property type="entry name" value="dsDNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015730">
        <property type="entry name" value="TFAR19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46950">
        <property type="entry name" value="Double-stranded DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2 3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="4"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95864.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="109" mass="12840" checksum="21A8CC7405A13F2A" modified="2013-06-26" version="1">MDIEEIRRKKMLELQKKLAEEEEKQKALLEAELQKRALIKRILTAEARERLERIRMARPEFAEAIELQLIQLAQLGRLPIPLTDEQFKALLEKIAAAKKKKSEIKIIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU81</accession>
    <name>N6UU81_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Nicotinamide-nucleotide adenylyltransferase</fullName>
            <ecNumber evidence="5 6">2.7.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">NAD(+) diphosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">NAD(+) pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">NMN adenylyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_05374</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>beta-nicotinamide D-ribonucleotide + ATP + H(+) = diphosphate + NAD(+)</text>
            <dbReference type="Rhea" id="RHEA:21360"/>
            <dbReference type="ChEBI" id="CHEBI:14649"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="EC" id="2.7.7.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from nicotinamide D-ribonucleotide: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the archaeal NMN adenylyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.1" evidence="5 6"/>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95909.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592394.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU81"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01312"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1049"/>
    <dbReference type="OrthoDB" id="264480at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00600"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000309">
        <property type="term" value="F:nicotinamide-nucleotide adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02166">
        <property type="entry name" value="NMNAT_Archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00243">
        <property type="entry name" value="NMN_adenylyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006418">
        <property type="entry name" value="NMN_Atrans_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01527">
        <property type="entry name" value="arch_NMN_Atrans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002243">
        <property type="entry name" value="PRK01153.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342:SF0">
        <property type="entry name" value="BIFUNCTIONAL NMN ADENYLYLTRANSFERASE_NUDIX HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21342">
        <property type="entry name" value="PHOSPHOPANTETHEINE ADENYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="5" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="7">
        <location>
            <begin position="5"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR01527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95909.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="173" mass="20294" checksum="A981AE3D4EF8BCDB" modified="2013-06-26" version="1">MRGFIIGRFQPFHKGHLEVIKELSKDVDEIIIGIGSAQKSHTLEDPFTAGERILMITKTLSKYDIIYYPIPIRDIEFNSIWVAYVKSLTPKFDIVYTGNPLVRVLFEEEGYKVKTPKFYNRKEYSGTEIRRRMLNDEPWEHLVPDEVVKVIKEIKGVERLKKLAKSDKYENKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUT3</accession>
    <name>N6UUT3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04533</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96104.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591842.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUT3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00618"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.889"/>
    <dbReference type="OrthoDB" id="85793at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96104.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="273" mass="32515" checksum="57DF55F1C0A15DCD" modified="2013-06-26" version="1">MKKDELISFIKNKVKTIRKELNLKNTDFDIVDVDIHDDILTIYTKNRSDKSIIIGPGGWVVGKLKEEIGFKVIRVEDFTDKIIFLERVKVIKEFNNDVLNDICNYFLYSVKSKREVYQLIQCQYDIYAYEILSKIFKVKPISYHHSALLPKKYEEKIKKFVKDIKFINIEIDIKRVIKNYPCGFLKEFIKKDFDNYVFTTCSNTDLKNKIINFFELFPIKIKRDEKYLNYCPLAIQSFKYHRKEFISKITLEVYKGFKEPTEAAEEILKMVKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUW7</accession>
    <name>N6UUW7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03783</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96149.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591347.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUW7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00389"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.741"/>
    <dbReference type="OrthoDB" id="358785at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43324">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43324:SF1">
        <property type="entry name" value="RADICAL SAM CORE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="176"/>
            <end position="441"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96149.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="500" mass="57537" checksum="8F846E2E3EDB3237" modified="2013-06-26" version="1">MRALIIDGYTDEPAGLGVPPYIGIYPRYAYGVLDKFGVETDYTTIDKFRNIRGKFNLDKYNIIIAICGFHTPGKYLRANPATLKEFVSILYKYNGIKILAGPAATKFGSSQIGGKIEDEKKYKDFFDIVIEGDVDAVIKDFLEFGLEKVDEERMRNYSELREFAIRGAKVVKQHPEYPYIIAEIETYRGCHRAVCGGCSFCVEPRRYGLPEFREEKDIIDEVKALMKEGVRYFRIGRQPCIYSYKAHDFDNPKPNVEAIERLFRGLNSLNPKVLHIDNANPYVIANYEESVEVTKILVKYCTPGNVAAFGVESFDEKVIKANNLNAYPEDVIKAVEILNKIGGKRGENGLPYLLPGINLLFGLKGERKETFNINYQYLKEIYDRGLMLRRINIRQVVPFFGTDITLKDLKKAEKRKKLFLWFKEKIRNDIDNKMLKRILPKGTVLKDVFIEIKKDNLYYGRQFGSYPILVGIKDNVRLREFVDVEVIDYGRRSITGKVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV95</accession>
    <name>N6UV95_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_02944</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96284.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV95"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.577"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="4">
        <location>
            <begin position="1"/>
            <end position="74"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96284.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="74" mass="8595" checksum="E3C21CBF8FFACAC8" modified="2013-06-26" version="1" fragment="single">PRFSLAGHLYSEEFYRELYRVLKPGGRLFHYVGNPGKKYRGKDLQKGVMERLRKVGFKEVKKVEKALGVVAKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVR6</accession>
    <name>N6UVR6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">3-isopropylmalate dehydratase large subunit</fullName>
            <ecNumber evidence="9">4.2.1.33</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Alpha-IPM isomerase</fullName>
            <shortName evidence="9">IPMI</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Isopropylmalate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">leuC</name>
        <name evidence="11" type="ORF">J422_02350</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate</text>
            <dbReference type="Rhea" id="RHEA:32287"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:35121"/>
            <dbReference type="EC" id="4.2.1.33"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 2/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Heterodimer of LeuC and LeuD.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.33" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96429.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590339.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVR6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00351"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.462"/>
    <dbReference type="OrthoDB" id="255at2157"/>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00071"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003861">
        <property type="term" value="F:3-isopropylmalate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01583">
        <property type="entry name" value="IPMI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.499.10">
        <property type="entry name" value="Aconitase, domain 3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01027">
        <property type="entry name" value="LeuC_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015931">
        <property type="entry name" value="Acnase/IPM_dHydase_lsu_aba_1/3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001030">
        <property type="entry name" value="Acoase/IPM_deHydtase_lsu_aba"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018136">
        <property type="entry name" value="Aconitase_4Fe-4S_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036008">
        <property type="entry name" value="Aconitase_4Fe-4S_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011826">
        <property type="entry name" value="HAcnase/IPMdehydase_lsu_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006251">
        <property type="entry name" value="Homoacnase/IPMdehydase_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050067">
        <property type="entry name" value="IPM_dehydratase_rel_enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033941">
        <property type="entry name" value="IPMI_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01343">
        <property type="entry name" value="hacA_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02086">
        <property type="entry name" value="IPMI_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001614">
        <property type="entry name" value="PRK00402.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822">
        <property type="entry name" value="HOMOACONITASE, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43822:SF22">
        <property type="entry name" value="ISOPROPYLMALATE_CITRAMALATE ISOMERASE LARGE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00330">
        <property type="entry name" value="Aconitase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00415">
        <property type="entry name" value="ACONITASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53732">
        <property type="entry name" value="Aconitase iron-sulfur domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00450">
        <property type="entry name" value="ACONITASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01244">
        <property type="entry name" value="ACONITASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 9" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="7 9 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase/3-isopropylmalate dehydratase large subunit alpha/beta/alpha" evidence="10">
        <location>
            <begin position="18"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="304"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="365"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96429.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="424" mass="46593" checksum="08CB662AAB7A03C6" modified="2013-06-26" version="1">MGMTIVEKILARASGKKEVSPGDIVMAKIDRAMVHDITGPLTVNVLREHNIEKVWDNERIVILFDHQVPADSIKAAENHKLMRNFVKKHNIKYFYDIREGVCHQVLPEKGFVAPGEVIVGADSHTCTHGAFGAFATGIGSTDMAHVFATGELWFKVPETIYFNINGELKEFVTSKDVILYIIGEMGVDGAVYKACQFGGETVRNMSIASRMTMTNMAIEMGGKTGIIEPDEKTINYVKEAMKKHGTEREFKVVKADEDAEYEEIIDIEADKIEPVVACPHNVDNVKPVREVAGKEIDQVFIGSCTNGRFEDLKMAADFIDKHGGIADNVRVIVTPASREEFLKALKAGVIEKFLKHGCVVTNPSCSACMGSLYGVLADYEVCVSTSNRNFRGRQGSLKAEIYLASPITAAACAVKGEIVDPRDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWA7</accession>
    <name>N6UWA7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PHP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01440</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000010">
        <property type="protein sequence ID" value="ENN96584.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589949.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWA7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01023"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.282"/>
    <dbReference type="OrthoDB" id="63337at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07432">
        <property type="entry name" value="PHP_HisPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052018">
        <property type="entry name" value="PHP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924">
        <property type="entry name" value="EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924:SF3">
        <property type="entry name" value="POLYMERASE_HISTIDINOL PHOSPHATASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13263">
        <property type="entry name" value="PHP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96584.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="237" mass="27660" checksum="86B36B65594270B9" modified="2013-06-26" version="1">MKVDIHVHSINSKCSLNPKSLLRKMCKKYNLIPAVCDHNKLTKLDFAIPGEEVATNRGEFLALFIEEEIPGNLDIFEALDQVKEQGGLICIPHPFDSKRKRSLIKFNILEDREFLRYVDIVEVFNSRCRSLEPNIKAFEYAIKYNFPMGFGSDAHFIWELNNAYINFPELNIDKPSEISPKEFLNLLEIRTKEFLKEKIDLFKNPWKGKNHYGALGDRKNIEIYSKLLKKVRKILNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWF3</accession>
    <name>N6UWF3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01106</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96649.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589889.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWF3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00821"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.216"/>
    <dbReference type="OrthoDB" id="65559at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="114"/>
            <end position="153"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96649.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="171" mass="20271" checksum="686390DE493FC2EA" modified="2013-06-26" version="1">MDVAYKIYPEEFLNNEVVDNCLILNNRKLKKIRVMGRVDIVGEGECYLEGVLIKGNLEGVKEGDIIDVIGYPRNKFIEAEIIKRRDEKWLNLRKLEIELTRKYIEFAEPFIEDKEELKRKIIEIINKLESVKFDELKDMLPISEEELEEIINELIEIGEIFEPRPRVYKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWI0</accession>
    <name>N6UWI0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Transcription elongation factor Spt4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="primary">spt4</name>
        <name evidence="4" type="ORF">J422_00896</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterodimer composed of Spt4 and Spt5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeal Spt4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96684.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589849.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWI0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00595"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.176"/>
    <dbReference type="OrthoDB" id="275101at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00949">
        <property type="entry name" value="Spt4_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029040">
        <property type="entry name" value="RPABC4/Spt4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022800">
        <property type="entry name" value="Spt4/RpoE2_Znf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007178">
        <property type="entry name" value="Spt4_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038589">
        <property type="entry name" value="Spt4_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041664">
        <property type="entry name" value="RNAP_arch_Epp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40704:SF1">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06093">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01389">
        <property type="entry name" value="Spt4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63393">
        <property type="entry name" value="RNA polymerase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Spt4/RpoE2 zinc finger" evidence="3">
        <location>
            <begin position="1"/>
            <end position="58"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="4"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM01389"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96684.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="59" mass="6632" checksum="F1923CC56CEBFF0B" modified="2013-06-26" version="1">MRACLKCKYLTTEETCPICGAQTSENWMGLLIIINPEKSEIAKKANIKVKGRYAITVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWN6</accession>
    <name>N6UWN6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 12">Aspartate-semialdehyde dehydrogenase</fullName>
            <shortName evidence="12">ASA dehydrogenase</shortName>
            <shortName evidence="12">ASADH</shortName>
            <ecNumber evidence="4 12">1.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Aspartate-beta-semialdehyde dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">asd</name>
        <name evidence="15" type="ORF">J422_00516</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the NADPH-dependent formation of L-aspartate-semialdehyde (L-ASA) by the reductive dephosphorylation of L-aspartyl-4-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-aspartate 4-semialdehyde + phosphate + NADP(+) = 4-phospho-L-aspartate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24284"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="1.2.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="12">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 2/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 2/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 12">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the aspartate-semialdehyde dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.11" evidence="4 12"/>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589785.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWN6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00055"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.99"/>
    <dbReference type="OrthoDB" id="38238at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00016"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00463"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00464"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004073">
        <property type="term" value="F:aspartate-semialdehyde dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071266">
        <property type="term" value="P:'de novo' L-methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18130">
        <property type="entry name" value="ASADH_C_arch_fung_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02315">
        <property type="entry name" value="ScASADH_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.360.10:FF:000016">
        <property type="entry name" value="Probable aspartate-semialdehyde dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02121">
        <property type="entry name" value="ASADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051823">
        <property type="entry name" value="ASADH-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005676">
        <property type="entry name" value="Asp_semi-ald_DH_pep-lack"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012080">
        <property type="entry name" value="Asp_semialdehyde_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000534">
        <property type="entry name" value="Semialdehyde_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012280">
        <property type="entry name" value="Semialdhyde_DH_dimer_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00978">
        <property type="entry name" value="asd_EA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006416">
        <property type="entry name" value="PRK08664.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46718:SF1">
        <property type="entry name" value="ASPARTATE-SEMIALDEHYDE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01118">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02774">
        <property type="entry name" value="Semialdhyde_dhC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000148">
        <property type="entry name" value="ASA_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00859">
        <property type="entry name" value="Semialdhyde_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="10 12" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="7 12" id="KW-0521">NADP</keyword>
    <keyword evidence="9 12 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0791">Threonine biosynthesis</keyword>
    <feature type="domain" description="Semialdehyde dehydrogenase NAD-binding" evidence="14">
        <location>
            <begin position="4"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="12 13">
        <location>
            <position position="150"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="12 13">
        <location>
            <position position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="11"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="38"/>
            <end position="39"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="177"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="180"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="206"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="236"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="326"/>
            <end position="327"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02121"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR000148-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN96754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="347" mass="38755" checksum="00C3CDFB882830F7" modified="2013-06-26" version="1">MKIKVGVLGATGLVGQRFVQLLADHPMFSLEVLAASERSAGKTYKEACNWYLSEGMPESVKDMTVIKTDPKAKEFEDVDIVFSALPSDLAKELEPKFAEDKLVFSNASAYRMEEDVPLIIPEVNEDHFALIDIQRDNRKWDGAIITNPNCSTICLCITLKPIMDNFGLESVFVSTMQAVSGAGYNGVPSMGILDNIIPYIKKEEEKMETESLKILGKFEDNKVKFADFKVSASCHRVPVIDGHLEAIFVKTSEGAEPEEIKEVMEKFDPLKDLDLPTYAKPIVIKEEEDRPQPRLDRNTGKGMSIVVGRIRKDKIFDVKYLALEHNTIRGAAGASVLNAELYVKKYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWU4</accession>
    <name>N6UWU4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS12</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rps12P</name>
        <name evidence="7" type="synonym">rps12</name>
        <name evidence="10" type="ORF">J422_00155</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">With S4 and S5 plays an important role in translational accuracy. Located at the interface of the 30S and 50S subunits.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7 9">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uS12 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589720.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWU4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01137"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.31"/>
    <dbReference type="OrthoDB" id="45154at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03367">
        <property type="entry name" value="Ribosomal_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000007">
        <property type="entry name" value="40S ribosomal protein S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00403_A">
        <property type="entry name" value="Ribosomal_uS12_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006032">
        <property type="entry name" value="Ribosomal_uS12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022863">
        <property type="entry name" value="Ribosomal_uS12_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005680">
        <property type="entry name" value="Ribosomal_uS12_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003254">
        <property type="entry name" value="PRK04211.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00982">
        <property type="entry name" value="uS12_E_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11652">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S12 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00164">
        <property type="entry name" value="Ribosom_S12_S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002133">
        <property type="entry name" value="Ribosomal_S12/S23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003622"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="148" mass="16807" checksum="2E9B4D18B8488F7A" modified="2013-06-26" version="1">MSGSKSPRGEFAGRKLRLKRKWCRWHDYRYVKRVLRLKEKYDPLEGAPMARGIVIEKVGLEAKQPNSGIRKCVRVQLIKNGRVVTAFCPGNRAITFIDEHDEVIIEGIGGPKGPRAKGDIPGVKYKVIMVGKNSLKELVRGRQEKIKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6UZZ8</accession>
    <name>N6UZZ8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase AAA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06967</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000065">
        <property type="protein sequence ID" value="ENN95608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594641.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UZZ8"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011579">
        <property type="entry name" value="ATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301:SF8">
        <property type="entry name" value="ATPASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34301">
        <property type="entry name" value="DNA-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01637">
        <property type="entry name" value="ATPase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="85" mass="10431" checksum="8A68F13D7F6F326B" modified="2013-06-26" version="1" fragment="single">MEFFNREKEINEILSIIEFEPNFIYFLYGPINSGKTALINEIINNRLDKDKYVVFYINLRRYFISKYNDFIEVLFEEYNENKKPV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6V037</accession>
    <name>N6V037_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Dihydropteroate synthase-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06718</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000057">
        <property type="protein sequence ID" value="ENN95658.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V037"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00708"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1308"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042558">
        <property type="term" value="P:pteridine-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005236">
        <property type="entry name" value="Dihydropt_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000489">
        <property type="entry name" value="Pterin-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025595">
        <property type="entry name" value="PterinBD-DUF4346"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00284">
        <property type="entry name" value="dihydropteroate synthase-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00809">
        <property type="entry name" value="Pterin_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14251">
        <property type="entry name" value="PterinBD-DUF4346"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50972">
        <property type="entry name" value="PTERIN_BINDING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pterin-binding" evidence="1">
        <location>
            <begin position="131"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50972"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95658.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="510" mass="58754" checksum="9D9FE90BB284D477" modified="2013-06-26" version="1">MAEKRVKEAVKKYDFIDIHVANISVAAFLTPNLIIKEIKNLEKKYNKKLNQIYDFVLVTGLIRHDLKKVEEETGIACYKSTREAADIPLLIENLDKIKLSTKDYADLQLIDIIKERAEKEIEAVEKEEFNHIKIGNLKVGDDYPIRVLGEIVHAPWLKKEELEKKVMYYVECGADMIDLGMVSNENNKDKIKEMVSIVKDIEDIPVSIDTMNIEELKEAINYADLILSLDRGNIDTLIPYLKDSDVASVVLPTDYKNNIIPKSPDDKIKILKENIEKLLYNNIGKIIADPILEPINEGFIDSLIAYKKFKEFYDFPTFFGAGNVSELLDADSNGVHALLTAIAYEVRANIIFSPEASAKCKFSIKELKTASKMMFLAKRRNSLPKDLGIDLINYKDKRFEEEITFKNYNIPIIDAKENEKTILDEGSFKIEIDRENKKIIAIYFKNNKPTLIIRGEKAKEIYDTIINLKLIKRLDHACYLGRELSKAEIALKIGKKYNQDFELFYNDFWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>N6V044</accession>
    <name>N6V044_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Enolase</fullName>
            <ecNumber evidence="6">4.2.1.11</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">2-phospho-D-glycerate hydro-lyase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">2-phosphoglycerate dehydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6 12" type="primary">eno</name>
        <name evidence="12" type="ORF">J422_06548</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the reversible conversion of 2-phosphoglycerate (2-PG) into phosphoenolpyruvate (PEP). It is essential for the degradation of carbohydrates via glycolysis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>(2R)-2-phosphoglycerate = phosphoenolpyruvate + H2O</text>
            <dbReference type="Rhea" id="RHEA:10164"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="EC" id="4.2.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="6">Binds a second Mg(2+) ion via substrate during catalysis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Mg(2+) is required for catalysis and for stabilizing the dimer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Secreted</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="6">Cell surface</location>
        </subcellularLocation>
        <text evidence="6">Fractions of enolase are present in both the cytoplasm and on the cell surface.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the enolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.11" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000055">
        <property type="protein sequence ID" value="ENN95668.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V044"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00609"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1274"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00187"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009986">
        <property type="term" value="C:cell surface"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005576">
        <property type="term" value="C:extracellular region"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000015">
        <property type="term" value="C:phosphopyruvate hydratase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004634">
        <property type="term" value="F:phosphopyruvate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03313">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.390.10:FF:000001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.120">
        <property type="entry name" value="Enolase-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.390.10">
        <property type="entry name" value="Enolase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00318">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000941">
        <property type="entry name" value="Enolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036849">
        <property type="entry name" value="Enolase-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020810">
        <property type="entry name" value="Enolase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020809">
        <property type="entry name" value="Enolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01060">
        <property type="entry name" value="eno"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11902:SF1">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00113">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03952">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001400">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00148">
        <property type="entry name" value="ENOLASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00002">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG00178">
        <property type="entry name" value="enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00001">
        <property type="entry name" value="Enolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01192">
        <property type="entry name" value="Enolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51604">
        <property type="entry name" value="Enolase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00164">
        <property type="entry name" value="ENOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="5 6 12" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0964">Secreted</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="11">
        <location>
            <begin position="11"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="domain" description="Enolase C-terminal TIM barrel" evidence="10">
        <location>
            <begin position="145"/>
            <end position="426"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="6 7">
        <location>
            <position position="213"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6 7">
        <location>
            <position position="339"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="289"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6 9">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="314"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="339"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="366"/>
            <end position="369"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="368"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="369"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>(2R)-2-phosphoglycerate</name>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="390"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001400-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95668.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="427" mass="47007" checksum="D9102F3556754DC5" modified="2013-06-26" version="1">MLYNVDERFNIKDVIAREVIDSRGNPTVEVEVITEGHGFGRAIVPSGASTGSHEALELRDKDQRFGGNGVLLAVENVNSIIKPEIIGYDARMQREIDTIMIELDGTENKSKLGANAILAVSLAVAKAAADTAKIPLYKYLGGCNSYIMPVPLMNIINGGKHAGNNLDIQEFMIMPVGANSIAEAVRMGSEVYHVLKQVIVEKYGKSAINVGDEGGFAPPLNETREALNLLLEAIKRANYEDEIVLALDAAATEFYKDGYYQIDGKKLTREQLLEFYKELVDEYPIVSIEDPFHEDDFEGFKIITEELDIQIVGDDIFVTNVKRLKRGIKERAGNALLLKVNQIGTLTEAIDAANLAYRNGYAVIVSHRSGETEDTTIADLSVALNSGQIKTGAPARGERTAKYNQLIRIEQELGMSKYAGRDFRCPF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6V065</accession>
    <name>N6V065_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutamine amidotransferase class-II</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06406</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593529.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V065"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01293"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1247"/>
    <dbReference type="OrthoDB" id="372195at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01907">
        <property type="entry name" value="GlxB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012375">
        <property type="entry name" value="Glu_synth_lsu_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00310">
        <property type="entry name" value="GATase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018774">
        <property type="entry name" value="GOGAT_lg_dom1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="active site" description="For GATase activity" evidence="1">
        <location>
            <position position="2"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR018774-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="362" mass="41476" checksum="82757D8FD6E7623F" modified="2013-06-26" version="1">MCGIIGFMSRKKRMINGEKIALALNSLRERGNGRGSGYVGYGIYPNKYKDCYALHVLIDNTPKFEKIKIAVENVLNECGTIVKDEEIPTEDGVIEKVHIPWRYFYEVDEKYQHMEEDIIVNIVMEINDKIDGAYVISSGKDLGVFKAVGWPDEVAEFYKIDKYDGYLWLAHARYPTNTKAWWGGAHPFNLLNWSVVHNGEITSYGTNRRFVEMYGYKCRLLTDTEVVTYILDLLLRKHKIPVEYGLFALAPKFWNEIDKLPEEEREVYKAIRMTYGGALLNGPFAIAVGTPEGLFFMNGEIEKSTTMIGLTDRIKLRPLVAAEKDDIVFVSSEESAIRRICPDLDRVWMPDAGEPIIVRPYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0B5</accession>
    <name>N6V0B5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker protein 5</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06175</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95763.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593269.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0B5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00554"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1203"/>
    <dbReference type="OrthoDB" id="52584at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012356">
        <property type="entry name" value="Methan_mark_5"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03271">
        <property type="entry name" value="methan_mark_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09885">
        <property type="entry name" value="DUF2112"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018781">
        <property type="entry name" value="UCP018781"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95763.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="160" mass="17638" checksum="11D4C5A0CD944E18" modified="2013-06-26" version="1">MKKVFIFPPNSLILTDLVERFGHKPLTLNIVIGKLVRNAEIDSPPINITPEEPKKGLKYAAIEVPSGVRGRLALIGPLIEEAEAAIIMEDAPIAFGCIGCQRTNELTLYLIRRKNIPILKVKYPTNEEEAEILVNKIVNFLKSLEGDGNGMSEGEKSGEM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0I5</accession>
    <name>N6V0I5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flagellin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05838</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592840.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0I5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01238"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1138"/>
    <dbReference type="OrthoDB" id="63667at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42200:SF2">
        <property type="entry name" value="ARCHAEAL FLAGELLA-RELATED PROTEIN F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42200">
        <property type="entry name" value="ARCHAEAL FLAGELLA-RELATED PROTEIN F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="135" mass="15211" checksum="44975ED33A93108C" modified="2013-06-26" version="1">MGFSSIAGTVIMIIALLICGAYLYSVIDEKTSKILTAQEEHFEYIYNKIHEKLVIVKVSKTPNQTNITVLNNGSTVLEPSKFSVIFDGNIVPSKNISYNPNKQYLFPLENITIIVNWTEPQRICIVSDTGNKYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0J7</accession>
    <name>N6V0J7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpl3p</name>
        <name evidence="6" type="synonym">rpl3</name>
        <name evidence="8" type="ORF">J422_05603</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins, it binds directly near the 3'-end of the 23S rRNA, where it nucleates assembly of the 50S subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Forms a cluster with proteins L14 and L24e.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592684.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0J7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00273"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1093"/>
    <dbReference type="OrthoDB" id="6121at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1430.10:FF:000005">
        <property type="entry name" value="50S ribosomal protein L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1430.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.960.10">
        <property type="entry name" value="Ribosomal protein L3, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01325_A">
        <property type="entry name" value="Ribosomal_uL3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045077">
        <property type="entry name" value="L3_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044892">
        <property type="entry name" value="Ribosomal_L3_dom_3_arc_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000597">
        <property type="entry name" value="Ribosomal_uL3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019928">
        <property type="entry name" value="Ribosomal_uL3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019926">
        <property type="entry name" value="Ribosomal_uL3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03626">
        <property type="entry name" value="L3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003261">
        <property type="entry name" value="PRK04231.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11363:SF5">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00297">
        <property type="entry name" value="Ribosomal_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00474">
        <property type="entry name" value="RIBOSOMAL_L3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="332" mass="38072" checksum="ADA332585F751300" modified="2013-06-26" version="1">MPKTKRPRRGSLAFSPRKRAKRPVPRIRSWPDEERVRLQAYPVYKAGMTHVFIKEDNQKSPRAGQEVFTAATILEAPPINVFGIRVYGRNERNYLTTLTEIWADEFDKELERKIKLPKKEDRKTVDYLDEIKDRIEDVRVLVHTNPKLTCLPKKKPEILEIRIGGKSLEERLNYAKQILGKQLNITDVFQEGELVDTIGVTKGKGFQGVVKRWGVKIQFGKHARQGVGRHVGSIGPWTPKMIMWTVPRPGQMGYHQRTEYNKRILKIGNNGEEITPKGGFPHYGVIRNNYVLLKGSVQGPAKRLIVLRSAIRPSEPLIKVPEITYISTTPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0P2</accession>
    <name>N6V0P2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Fumarate hydratase</fullName>
            <ecNumber evidence="8">4.2.1.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_05424</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the class-I fumarase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.2" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000039">
        <property type="protein sequence ID" value="ENN95883.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592478.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0P2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00840"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1059"/>
    <dbReference type="OrthoDB" id="371925at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004333">
        <property type="term" value="F:fumarate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051208">
        <property type="entry name" value="Class-I_Fumarase/Tartrate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004646">
        <property type="entry name" value="Fe-S_hydro-lyase_TtdA-typ_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004885">
        <property type="entry name" value="PRK06246.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00722">
        <property type="entry name" value="ttdA_fumA_fumB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30389">
        <property type="entry name" value="FUMARATE HYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30389:SF17">
        <property type="entry name" value="L(+)-TARTRATE DEHYDRATASE SUBUNIT ALPHA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05681">
        <property type="entry name" value="Fumerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe-S hydro-lyase tartrate dehydratase alpha-type catalytic" evidence="7">
        <location>
            <begin position="3"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008876"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF05681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95883.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="278" mass="30551" checksum="D92CA5D62665B972" modified="2013-06-26" version="1">MREIIVDLFREAVIYLPDDVKRALKEAYERESSKLAKDTLKAILENIEIAERKRIPLCQDTGVPIVYLKIGKGIESDKIFDIIKEIEKGVEIATKLVPLRPNVVNPITRKNLGNVGLGMPIIDVEFDDNLNKEIEITVFPKGAGSENMSKLKMLKPSEGLEGVKRFILESIAEASGKPCPPIIVGVGIGGTADYAMKLAKKALLRDIGERNKDDMIANLELELLEKINNLGIGAMGLGGDITALDVFIETAGCHTASLPVGLCIQCWAHRKAKRRLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0X2</accession>
    <name>N6V0X2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Ribosomal RNA small subunit methyltransferase Nep1</fullName>
            <ecNumber evidence="10">2.1.1.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">16S rRNA (pseudouridine-N1-)-methyltransferase Nep1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">nep1</name>
        <name evidence="11" type="ORF">J422_04905</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Methyltransferase involved in ribosomal biogenesis. Specifically catalyzes the N1-methylation of the pseudouridine corresponding to position 914 in M.jannaschii 16S rRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>a pseudouridine in rRNA + S-adenosyl-L-methionine = an N(1)-methylpseudouridine in rRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46696"/>
            <dbReference type="Rhea" id="RHEA-COMP:11634"/>
            <dbReference type="Rhea" id="RHEA-COMP:13933"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="2 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase NEP1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.-" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95953.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592077.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0X2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00510"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.961"/>
    <dbReference type="OrthoDB" id="7612at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070037">
        <property type="term" value="F:rRNA (pseudouridine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18088">
        <property type="entry name" value="Nep1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000042">
        <property type="entry name" value="Ribosomal RNA small subunit methyltransferase Nep1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00554">
        <property type="entry name" value="NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005304">
        <property type="entry name" value="Rbsml_bgen_MeTrfase_EMG1/NEP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023503">
        <property type="entry name" value="Ribosome_NEP1_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003204">
        <property type="entry name" value="PRK04171.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636">
        <property type="entry name" value="NEP1/MRA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12636:SF5">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE NEP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03587">
        <property type="entry name" value="EMG1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="9 10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 10" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="8 10" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="7 10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6 10" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <position position="161"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="166"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="182"/>
            <end position="187"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="10">
        <location>
            <position position="53"/>
        </location>
    </feature>
    <feature type="site" description="Stabilizes Arg-xx" evidence="10">
        <location>
            <position position="55"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="10">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="10">
        <location>
            <position position="97"/>
        </location>
    </feature>
    <feature type="site" description="Interaction with substrate rRNA" evidence="10">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95953.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="203" mass="23875" checksum="2B48F6EE35A452F1" modified="2013-06-26" version="1">MYNIILANSALELIPKEIKNKIKPSKVYKYDILDANYHYKAMKFLKDRERRGRPDIVHVSLLTILDSPLNHERMLNTYVHTYDNKVLYIDPKTRLPRNYFRFLGVMEKVLKGYKNPLIKLEKKTLEELLFDINAKNIALLTKKGKVININDMKRYDTFLIGGFPYGEIRINEEGLNIDKISLYNKGLMAWTVCGIVCYSLYFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>N6V128</accession>
    <name>N6V128_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Diaminopimelate epimerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05130</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95998.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V128"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.10">
        <property type="entry name" value="Diaminopimelate Epimerase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54506">
        <property type="entry name" value="Diaminopimelate epimerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95998.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="30" mass="3608" checksum="1535EC446E66928C" modified="2013-06-26" version="1" fragment="single">MEFTKMQALGNDYIVIDEFNEEKIEESKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6V161</accession>
    <name>N6V161_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_04760</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96023.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592001.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V161"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00971"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.931"/>
    <dbReference type="OrthoDB" id="8919at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd17784">
        <property type="entry name" value="CBS_pair_Euryarchaeota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051462">
        <property type="entry name" value="CBS_domain-containing"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48108:SF33">
        <property type="entry name" value="METHYLATED PROTEIN MJ0556"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="13"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="3">
        <location>
            <begin position="77"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96023.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="137" mass="15391" checksum="A9591F669400745B" modified="2013-06-26" version="1">MFNIKNIKVKDIMTRNVITAKKDESVIEAFEKMLKYKISSLPVVENNKVIGIVTTTDIGYNLIKDKYTLTTTVGEVMTKNVVTINEDASILEAIKKMDEYGDKDEIINQLPVVDKDNNLVGIISDGDIIRALSKIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6V180</accession>
    <name>N6V180_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="1">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_04615</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000029">
        <property type="protein sequence ID" value="ENN96038.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591921.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V180"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01414"/>
    <dbReference type="REBASE" id="68615">
        <property type="entry name" value="M.MviT80ORF4615P"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.904"/>
    <dbReference type="OrthoDB" id="45790at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050953">
        <property type="entry name" value="N4_N6_ade-DNA_methylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33841:SF1">
        <property type="entry name" value="DNA METHYLTRANSFERASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33841">
        <property type="entry name" value="DNA METHYLTRANSFERASE YEEA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA methylase adenine-specific" evidence="5">
        <location>
            <begin position="317"/>
            <end position="444"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00047942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96038.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="1166" mass="135703" checksum="98859DF16B3B984B" modified="2013-06-26" version="1">MGTHSEKITERTLYPLLLEIFKDIGFTGVQEIKVAGRRYPDLKISYKDTAFYVQVKIGKLDNIRDILTDISKLVSNKVISDINGFIGIKFDENIRNVKLITGHEEEVLKTAIENSKVYIIVSGSKNKELLFHMIDGNLEFKNAKKELINKIREFIDETKTIVNPESIVRVLKEYIDELAYILRLYYLSSPEALINLVGDISLFKSLLGLSESENLDELLRDEDFRTAVVDLLAFILTNQILFYYLYSNRSNKELPKLERITSLLDLKRYFNEITKIDFRAIYSINIFERLPNDKRILEVINNIIDVLNSNPIWKIPHDLLGRVYHKLLPFKTRKVFATFYTHPIAAEILARLTIDNYQESVIDPACGSGTLLVASYNTKKDLYRKYYGFISVEDDIKLHRQFVERDISGIDIMPFAAHIAAMNLSLQNIDAETNRLRISVDDSINILKRLLENKGRQEYLVLETVSSELIKVVESLKNRQTTLDAFFNGIKRRNFNITIYVYDTEHAKEYIISNKIDRKVIYVNNNENIEDINYNQIAILGISKNNLDKLINILKKLENIKDKFRNLTLNIVVDKNLSEEVISIVNRVFYNLDTDVKIKTIDVFTLTSADSIIMNPPFSDREKLPEEYREKLNQLNYYKFIKENVGGQINLWGYFLALSHFLLKSNCKVGAVIPINIARGKATEKIRKFFLKNYHIRWLVKPVADLAFSEAAAFRDILFIAEKRKPKEDDITGIVFIKKSIRSSEFTTEEAKRIVDELKLLYKKAKEGEINHYEDPDGFYEVYFVNYKTLIEHQENLMPLLKETGLEEDLMEVILERAGEKLERLDESYIRQGFHTSPAGLSELVYITRPLDKSRIGRNVIMVLHSEDSAFIYVVFKDKIKDLLKNFSGNNLEEFIKNHTKPDLKIPKDRVLPGLRTITGIKTIDITNKHDYIVIEDFMGFDKVVLMSKWKDKSLAKDPTTRRRFWDLIKKKLENQLVYTATPEKFNIYSINTSVICVVSKVKTIYNQTFRVNTADNIIFSLTYTLSFNSILGLTQVIRRISEATGEFVHLSTKDLETIYILDSNKLSDQEKQVLLNLFKEIKDIEFPSIMEQMKKGYEYIKHFKDKSWEDLLDYTIKNDPGMYARIKLDYTILKIVGFNEDEIKDLLEKTYKTIYEELIKIKSAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1F0</accession>
    <name>N6V1F0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF5518 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04443</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96088.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591808.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1F0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00635"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.871"/>
    <dbReference type="OrthoDB" id="66019at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040493">
        <property type="entry name" value="DUF5518"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17647">
        <property type="entry name" value="DUF5518"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="55"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="89"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96088.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="125" mass="12718" checksum="6407A60281DD69E0" modified="2013-06-26" version="1">MVNFDKEKMLNPSIIGGIINGILGAICCLGYIIGGAIAAHLYVNAGGSLDYENCGIVGAVSGVIGGIIAAVLNFFVMASLMPMFGGAKAFVIAGSSFIIMLISAIIFGAILGAVGGFIYVIIKNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1U9</accession>
    <name>N6V1U9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cl-channel voltage-gated family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_03378</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96268.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590983.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1U9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01061"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.663"/>
    <dbReference type="OrthoDB" id="89900at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034707">
        <property type="term" value="C:chloride channel complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005254">
        <property type="term" value="F:chloride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00400">
        <property type="entry name" value="Voltage_gated_ClC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3080.10">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014743">
        <property type="entry name" value="Cl-channel_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001807">
        <property type="entry name" value="ClC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050368">
        <property type="entry name" value="ClC-type_chloride_channel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43427">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43427:SF6">
        <property type="entry name" value="CHLORIDE CHANNEL PROTEIN CLC-E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00654">
        <property type="entry name" value="Voltage_CLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00762">
        <property type="entry name" value="CLCHANNEL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81340">
        <property type="entry name" value="Clc chloride channel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="8" id="KW-0868">Chloride</keyword>
    <keyword evidence="7" id="KW-0869">Chloride channel</keyword>
    <keyword evidence="9" id="KW-0407">Ion channel</keyword>
    <keyword evidence="5" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="44"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="132"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="167"/>
            <end position="187"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="207"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="246"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="275"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="306"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="333"/>
            <end position="356"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96268.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="390" mass="42580" checksum="9D01B759D1C0516A" modified="2013-06-26" version="1">MQKIKNILTIIKWTILSAAIGLVGGLSSLLMAIIIKYFPEKNNIFLIPIIFFISGFFVDKIYELKGSGVDRVLKALNYNINLSWKIGILKTILSSLVIAVGGSCGKEGPCVQSSASFADEIHRMLKLKNRELVIITGIAGGLSGAFSAPLGTAILACEIIEKEDFRYVNLIPPMMASVFSYAVFYVITHKKHLFEYLPHYCFDPIDIFWFLFGAIFCSIIASFYIKTYKTISNYFDNITIPYAIKSLLGGIVVATIGYFLPEILGMGVILVKELFMIKFSLIFLLFLLIGKVLATSFTVGSGTPGGLVFPSICVGAISGALFSSIFALNPTPFIVLGMATALSASLNAPLGSAVLCTEMFGFDFAIPSSIGAVIGYQVTRFETVFKYVRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6V255</accession>
    <name>N6V255_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02754</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96363.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590606.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V255"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00102"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.540"/>
    <dbReference type="OrthoDB" id="372228at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96363.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="208" mass="24101" checksum="A06512240B9ABF05" modified="2013-06-26" version="1">MEKLIRLINHEVWIEIEDRGYLKWGHYPKTDGKLDPLAITRAFYVKDGKYYPLVVGKDKISSSKEALFLEFDEADALGIEYNKGIYSLTEDNKWIFGKNIPDKYKIKETREIIGYSKVYLNKKIEPLGFELEIIPDKIDKKIKVDVIFRGKQVPAEITLRNKNGEFKFNSNNEIKLAEGINVLSARYVDTLSNNVDKRHLVSTITINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2C5</accession>
    <name>N6V2C5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Elongation factor 1-beta</fullName>
            <shortName evidence="7">EF-1-beta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">aEF-1beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">ef1B</name>
        <name evidence="7" type="synonym">ef1b</name>
        <name evidence="9" type="ORF">J422_02554</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Promotes the exchange of GDP for GTP in EF-1-alpha/GDP, thus allowing the regeneration of EF-1-alpha/GTP that could then be used to form the ternary complex EF-1-alpha/GTP/AAtRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the EF-1-beta/EF-1-delta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96418.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590469.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2C5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00762"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.500"/>
    <dbReference type="OrthoDB" id="84643at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00292">
        <property type="entry name" value="EF1B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00043">
        <property type="entry name" value="EF1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036219">
        <property type="entry name" value="eEF-1beta-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014038">
        <property type="entry name" value="EF1B_bsu/dsu_GNE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014717">
        <property type="entry name" value="Transl_elong_EF1B/ribsomal_bS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004542">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00489">
        <property type="entry name" value="aEF-1_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001670">
        <property type="entry name" value="PRK00435.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39647:SF1">
        <property type="entry name" value="ELONGATION FACTOR 1-BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00736">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006521">
        <property type="entry name" value="Transl_elong_EF1B_B_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00888">
        <property type="entry name" value="EF1_GNE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54984">
        <property type="entry name" value="eEF-1beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Translation elongation factor EF1B beta/delta subunit guanine nucleotide exchange" evidence="8">
        <location>
            <begin position="3"/>
            <end position="90"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007411"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017600"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96418.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="90" mass="9959" checksum="604F8C7C6E9ED8EC" modified="2013-06-26" version="1">MAKVLARIKIMPKSPEVDKEALKKKIEEVISKLDDVALRGIKDEPVAFGLYAIYIVIEMEEKEGGTEPIENALSEIDDVESVETVEVSLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2D7</accession>
    <name>N6V2D7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Thiamine thiazole synthase</fullName>
            <ecNumber evidence="6">2.4.2.59</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">thi4</name>
        <name evidence="7" type="ORF">J422_02345</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of the thiazole moiety of thiamine. Catalyzes the conversion of NAD and glycine to adenosine diphosphate 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylate (ADT), an adenylated thiazole intermediate, using free sulfide as a source of sulfur.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>hydrogen sulfide + glycine + NAD(+) = ADP-5-ethyl-4-methylthiazole-2-carboxylate + nicotinamide + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55704"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17154"/>
            <dbReference type="ChEBI" id="CHEBI:29919"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:139151"/>
            <dbReference type="EC" id="2.4.2.59"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; thiamine diphosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homooctamer; tetramer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the THI4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.59" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96428.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590337.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2D7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00350"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.461"/>
    <dbReference type="OrthoDB" id="4240at2157"/>
    <dbReference type="UniPathway" id="UPA00060"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00304">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002922">
        <property type="entry name" value="Thi4_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022828">
        <property type="entry name" value="Thi4_prok"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00292">
        <property type="entry name" value="sulfide-dependent adenosine diphosphate thiazole synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43422:SF3">
        <property type="entry name" value="THIAMINE THIAZOLE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01946">
        <property type="entry name" value="Thi4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00419">
        <property type="entry name" value="ADXRDTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <begin position="56"/>
            <end position="57"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="153"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="155"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="6">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <note>ligand shared between two adjacent protomers</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>glycine</name>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022977"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96428.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="254" mass="27609" checksum="154A8564833EC100" modified="2013-06-26" version="1">MLKADEVKITKVILKSSFEMWLSNIEVDVVIVGAGPSGLTCGRYLAKNGYKTLILERHLAFGGGTWGGGMGFPYIVIEPPANEILEECGVKLKEMEGYYVADSVEVPAKLACKAIDDGAKILTGVVVEDLIVRDHEVNGVVINSYAIERAGLHIDPLAIKSKVVVDATGHEANVVNILIRKNKLKLNLEGERSMWAEKGENELLENTKEVYPNLFVCGMAANAVFGGHRMGAIFGGMYLSGKKCAELIMEKLKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2G1</accession>
    <name>N6V2G1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Tetrahydromethanopterin S-methyltransferase subunit G</fullName>
            <ecNumber evidence="10">7.2.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit G</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">mtrG</name>
        <name evidence="11" type="ORF">J422_02150</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="7.2.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="10">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the MtrG family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.1.4" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96453.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590232.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2G1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01178"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.422"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01500">
        <property type="entry name" value="MtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005866">
        <property type="entry name" value="THM_MeTrfase_su_G"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01149">
        <property type="entry name" value="mtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04210">
        <property type="entry name" value="MtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006500">
        <property type="entry name" value="MtrG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="8 10" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3 10 11" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2 10" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="6 10" id="KW-1278">Translocase</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="43"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96453.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="77" mass="8757" checksum="CC2EBB6D021EAD98" modified="2013-06-26" version="1">MAVLTPSKEFKELMKKLDELEEKVENTNAEIFQRAGKKVGRDIGILYGLVLGIIIFSVIPYLLKFILYLTKVTVVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2P4</accession>
    <name>N6V2P4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Collagenase PrtC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_01880</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the peptidase U32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96523.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590133.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2P4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00224"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.370"/>
    <dbReference type="OrthoDB" id="51464at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001539">
        <property type="entry name" value="Peptidase_U32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051454">
        <property type="entry name" value="RNA/ubiquinone_mod_enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30217">
        <property type="entry name" value="PEPTIDASE U32 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30217:SF6">
        <property type="entry name" value="TRNA HYDROXYLATION PROTEIN P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01136">
        <property type="entry name" value="Peptidase_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01276">
        <property type="entry name" value="PEPTIDASE_U32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0645">Protease</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00038374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96523.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="403" mass="46343" checksum="66DE8D8517A0565F" modified="2013-06-26" version="1">MELLSPAGDMTCLRTAIKYGADAVYCGLKEFSMRKTAKNFTRDELKEAVKYVHDNDKKIYLCLNTVIYEDELDKAIDIINFAHSIDIDALILSDISLMEYANSLGIDVHASVQCNITNSNLAKFYSRYCKRIVLSRELTLNQIKIIKENLKRDGVNLELEGFVHGALCVAISGRCFLSAYLFNKHANCGECLQPCRRKWKLINEHHDGTYEILCEGKFLLSPKDLCMIEHIPELIGVLDAFKIEGRAKNADYVMRVTKVYREAIDSYFDGSYYDKLEYFKKELSKVYNRGYDTGFYFRDVSNKIDFQYDREGNISEYKKVEIGRVVNFYKKINVAEIELYHDLKVGDVILIMGKTTGCVEEKVDSMEINHKKVKIAKKGERVGVKLNNLVREGDKVYLLKKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2U4</accession>
    <name>N6V2U4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Reverse gyrase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="19" type="ORF">J422_01650</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process, increasing the linking number in steps of +1. Binds to single-stranded DNA, transiently cleaves and then rejoins the ends, introducing a positive supercoil in the process. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate. Involved in rewinding DNA strands in regions of the chromosome that have opened up to allow replication, transcription, DNA repair and/or for DNA protection.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="21">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">In the N-terminal section; belongs to the DEAD box helicase family. DDVD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000012">
        <property type="protein sequence ID" value="ENN96558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590021.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2U4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00323"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.325"/>
    <dbReference type="OrthoDB" id="30963at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160097">
        <property type="term" value="F:reverse gyrase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18798">
        <property type="entry name" value="SF2_C_reverse_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00186">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03361">
        <property type="entry name" value="TOPRIM_TopoIA_RevGyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.510.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.460.10">
        <property type="entry name" value="Topoisomerase I, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.290.10">
        <property type="entry name" value="Topoisomerase I, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01125">
        <property type="entry name" value="Reverse_gyrase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005736">
        <property type="entry name" value="Reverse_gyrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003601">
        <property type="entry name" value="Topo_IA_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013497">
        <property type="entry name" value="Topo_IA_cen"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013824">
        <property type="entry name" value="Topo_IA_cen_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013826">
        <property type="entry name" value="Topo_IA_cen_sub3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023405">
        <property type="entry name" value="Topo_IA_core_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003602">
        <property type="entry name" value="Topo_IA_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034142">
        <property type="entry name" value="TOPRIM_RevGyr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01054">
        <property type="entry name" value="rgy"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43505">
        <property type="entry name" value="REVERSE GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43505:SF1">
        <property type="entry name" value="REVERSE GYRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01131">
        <property type="entry name" value="Topoisom_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00417">
        <property type="entry name" value="PRTPISMRASEI"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00437">
        <property type="entry name" value="TOP1Ac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00436">
        <property type="entry name" value="TOP1Bc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56712">
        <property type="entry name" value="Prokaryotic type I DNA topoisomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52039">
        <property type="entry name" value="TOPO_IA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="11 14" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="12 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="15" id="KW-0472">Membrane</keyword>
    <keyword evidence="4 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14" id="KW-0799">Topoisomerase</keyword>
    <keyword evidence="15" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="15" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <keyword evidence="6 14" id="KW-0863">Zinc-finger</keyword>
    <feature type="transmembrane region" description="Helical" evidence="15">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase ATP-binding" evidence="17">
        <location>
            <begin position="1"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="Toprim" evidence="16">
        <location>
            <begin position="454"/>
            <end position="616"/>
        </location>
    </feature>
    <feature type="domain" description="Topo IA-type catalytic" evidence="18">
        <location>
            <begin position="637"/>
            <end position="1023"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="19">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00043976"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004026"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS52039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ENN96558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="1023" mass="119617" checksum="0FA038016FD619CF" modified="2013-06-26" version="1" fragment="single">KNKSFSIVAPTGVGKSFFGILMSLYLAKKGKKCYIILPTTLLVKQTYERLRGLDNNIDVAVYHSELSNKDKKEMKEKIEKGEYSILITTSNYLAKNMPKNKFDFIFVDDVDALLKASKNIDRTLMLLGFPEDIINEAAKIIYLIRIGKLEEAMKKREKLKEKIKSIKHGCLVIASATGKSYGDRVKLYRELLDFEVGFGMNKLRDVEDLYDNNFDLNRILNYVKKFGSGGLIFVSLDYGIEKAKKIEELLIKNNIKAKLIHSKDKKGFEDFKEEKIDVLIGVASYYGVLVRGLDMPERVRYTIFYGIPKFKMKLKEYIENLKEKKQIKEDIDIKNKDVKEIKKIIKEKLKIKNFSIRKEDDLYILFPDVKTYIQASGRTSRMTEFGLTKGLSVVLVDDEEIFEYLRKYMLFMYESEFKSVDEVCLDEILKKIDDDREKIKKGRKENKIPDLLKSVLMVVESPNKARTIANFFGNPSVRKINNRNVYEVCVGDLNLIITASGGHVFDLVTKEGFYGVKIEKNLYIPIYCSIKKINGEQYTDQKDIEELIKELLNKGEKVNFMDSKENIKIIREIADEVDAVFIATDIDNEGEKIGYDIALNIMPFNKNIYRIGFNEITKRAILRAVEAYKKGEKLELDKNKVKSQIVRRIEDRWIGFRLSQKLWNVFGKNYLSAGRVQTPVLGWIIERYNEYMERVKYLSLKLEYNLYVGKAIDEDFDKDYVNVDIKIYEKSIPPQPPFTTDTLLEEATKRFNLSVSEVMNIAQELFELGLTTYHRTSSTRVSLDGMRVAKEYLKLNNMEDYIKNREFFMEGAHECIRPTKPMDTEELIEFLKENNIKLTKNHIKVYDLIFRRFMASQMKDVVVDYEEIYIKDLDEKIEGYIEIKFDGWSRIYNLKLKQIPKIEKDKLKVLEKKIRKVPKVPLYDEGDVIKLMKERGIGRPSTYAQIIKKLLDRRYVIKSKDKNKLIPTKLGIEVYNYLISNYPHLISEERTRQLEEVMDKIERGEVNYIKALDELYEEILSIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2X4</accession>
    <name>N6V2X4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein eL20</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl18a</name>
        <name evidence="3" type="synonym">rpl20e</name>
        <name evidence="3" type="synonym">rplX</name>
        <name evidence="5" type="ORF">J422_01610</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="3">Part of the 50S ribosomal subunit. Binds 23S rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL20 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96578.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589981.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2X4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00449"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.317"/>
    <dbReference type="OrthoDB" id="191241at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00273">
        <property type="entry name" value="Ribosomal_eL20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028877">
        <property type="entry name" value="Ribosomal_eL20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023573">
        <property type="entry name" value="Ribosomal_eL20_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001981">
        <property type="entry name" value="PRK00773.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01775">
        <property type="entry name" value="Ribosomal_L18A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160374">
        <property type="entry name" value="RplX-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL20" evidence="4">
        <location>
            <begin position="3"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96578.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="72" mass="8543" checksum="26779D37FC9AE5E3" modified="2013-06-26" version="1">MPFYRITGKVLKKEPMFFRKEYKALKPEHAIELLYSEFGGLYKVKRSKIKIEKVEEIPPEEVTDPILKALIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6V311</accession>
    <name>N6V311_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Photosystem I assembly BtpA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01273</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the BtpA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96628.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589918.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V311"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00035"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.249"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04722">
        <property type="entry name" value="TIM_phosphate_binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005137">
        <property type="entry name" value="BtpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00259">
        <property type="entry name" value="thylakoid_BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21381:SF3">
        <property type="entry name" value="SGC REGION PROTEIN SGCQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21381">
        <property type="entry name" value="ZGC:162297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03437">
        <property type="entry name" value="BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005956">
        <property type="entry name" value="BtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96628.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="252" mass="28348" checksum="A51A221A31F4CF77" modified="2013-06-26" version="1">MLIGMVHLKPLPGSYLYEDNLDDVIDFAIKEAKKLEEANFDAVLIENFNDKPFKKEIDKITLASFTVIAKAIKEEVGMKIGINVLRNDSIASYSIAYAIKADFIRVNVLNGVAITDQGIIEGNAHELFRLKKLVPSKIKIYADVHVKHSYQFIDFETSLLDTYERALADAIIISGRRTGEEVNIEDVKLAKKYNIPTIIGSGVNEENIKKFINLADGFIIGSYIKRNGNVNNEIDINRAKRIAELFKSLRGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6V314</accession>
    <name>N6V314_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">PP-loop domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_01298</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96633.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589923.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V314"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00030"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.254"/>
    <dbReference type="OrthoDB" id="33422at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF27">
        <property type="entry name" value="TRNA-5-METHYLURIDINE(54) 2-SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <keyword evidence="2" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Rhodanese" evidence="3">
        <location>
            <begin position="39"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="3"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="16"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="278"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96633.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="293" mass="34364" checksum="F10B8EC5FB8C9597" modified="2013-06-26" version="1">MGCKLCGKEEFYKGLCKEHYINYFENLVKKSIKKYSMLKDSEKILVSVSGGKDSHAVAWVLKKLGYDIELFHINLGIKNFSEISLKEVKKLSNILDLPLHVVNLKDITGKTIENVKKKHCSICGITKRYLMNRFGYENGFDVIVTGHNLDDEVSFMINNILNWDIVHLSKHDPVLPAHDKFLKKVKIFYEIEEELILKYALAENIPFTTAECKYRKDAITLRHRMYFNELKKYRENIKLQFLHGYLKHKDIFKVDDNFKFRECEICGMTSAGKICSFCRVWKIKKNFKLQSQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6V349</accession>
    <name>N6V349_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01031</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000006">
        <property type="protein sequence ID" value="ENN96678.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V349"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01012"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.201"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96678.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="78" mass="9428" checksum="9864B72DCF09F2B9" modified="2013-06-26" version="1">MVIIYLYKLKSTKGDVMINEEKFKEIIKKMRDKYVNMWEKEKDEIIKNIEDAKKILLDSLEFNTILTDEEIEKNQKIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6V376</accession>
    <name>N6V376_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Large ribosomal subunit protein eL37</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11 13" type="primary">rpl37e</name>
        <name evidence="13" type="ORF">J422_00651</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Component of the large ribosomal subunit. The ribosome is a large ribonucleoprotein complex responsible for the synthesis of proteins in the cell.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11 12">Belongs to the eukaryotic ribosomal protein eL37 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96708.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589808.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V376"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00904"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.125"/>
    <dbReference type="OrthoDB" id="5619at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.30:FF:000003">
        <property type="entry name" value="50S ribosomal protein L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00547">
        <property type="entry name" value="Ribosomal_eL37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001569">
        <property type="entry name" value="Ribosomal_eL37"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011331">
        <property type="entry name" value="Ribosomal_eL37/eL43"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018267">
        <property type="entry name" value="Ribosomal_eL37_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003214">
        <property type="entry name" value="PRK04179.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10768:SF0">
        <property type="entry name" value="RIBOSOMAL PROTEIN L37"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01907">
        <property type="entry name" value="Ribosomal_L37e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01077">
        <property type="entry name" value="RIBOSOMAL_L37E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 11 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11 12" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 11 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7 11 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 11 12" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="11">
        <location>
            <begin position="20"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009805"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00035225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU000576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96708.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="61" mass="7257" checksum="547002F9959D4C1E" modified="2013-06-26" version="1">MSKGTPSMGKRNKGSYHIRCRRCGRRAYHVRKKRCAACGYPNPRMRKYSWQNKKVNGKRVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6V384</accession>
    <name>N6V384_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NifC-like ABC-type porter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_00721</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cell membrane</location>
            <topology evidence="7">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the binding-protein-dependent transport system permease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96718.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589818.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V384"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00892"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.139"/>
    <dbReference type="OrthoDB" id="11163at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015419">
        <property type="term" value="F:ABC-type sulfate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006469">
        <property type="entry name" value="NifC_ABC_porter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005667">
        <property type="entry name" value="Sulph_transpt2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01581">
        <property type="entry name" value="Mo_ABC_porter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406">
        <property type="entry name" value="SULFATE TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30406:SF8">
        <property type="entry name" value="SULFATE TRANSPORT SYSTEM PERMEASE PROTEIN CYST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0764">Sulfate transport</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 7" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="9"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="52"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="88"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="208"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="228"/>
            <end position="252"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="8">
        <location>
            <begin position="50"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96718.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="266" mass="29387" checksum="3A0E08C6831134B0" modified="2013-06-26" version="1">MQVISFRKFCIFIALLLTSLLFIAIISLFLVPNPKDVLSSIRTKEMIYSLKLSIITSLISTFTVMLLSIPAGYALSRYSFKGKNMVKSILDLPIAFPELVLGLALLLLFGQTYIGKFLESIGIHVVFTKLGIIVAQVFTALPYGIRIIYSTFEEISPRYEFVSRALGYNELETFINVTLPLAKNGLFASTIITFARCMGAFGAVLILAGGSYCYTETLPITLYLNISYGNIGMAITSGIVLVIISFIAIFAFEKLESSMRKGENKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3L6</accession>
    <name>N6V3L6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Acetolactate synthase small subunit</fullName>
            <shortName evidence="6">AHAS</shortName>
            <shortName evidence="6">ALS</shortName>
            <ecNumber evidence="6">2.2.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acetohydroxy-acid synthase small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ilvH</name>
        <name evidence="8" type="ORF">J422_00335</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of 2 pyruvate molecules into acetolactate in the first common step of the biosynthetic pathway of the branched-amino acids such as leucine, isoleucine, and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2 pyruvate + H(+) = (2S)-2-acetolactate + CO2</text>
            <dbReference type="Rhea" id="RHEA:25249"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58476"/>
            <dbReference type="EC" id="2.2.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 6">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Dimer of large and small chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 6">Belongs to the acetolactate synthase small subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.6" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589754.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3L6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01096"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.67"/>
    <dbReference type="OrthoDB" id="85792at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00055"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00059"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003984">
        <property type="term" value="F:acetolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990610">
        <property type="term" value="F:acetolactate synthase regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04878">
        <property type="entry name" value="ACT_AHAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1150:FF:000001">
        <property type="entry name" value="Acetolactate synthase small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000001">
        <property type="entry name" value="Acetolactate synthase, small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004789">
        <property type="entry name" value="Acetalactate_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019455">
        <property type="entry name" value="Acetolactate_synth_ssu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039557">
        <property type="entry name" value="AHAS_ACT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054480">
        <property type="entry name" value="AHAS_small-like_ACT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00119">
        <property type="entry name" value="acolac_sm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008864">
        <property type="entry name" value="PRK11895.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30239:SF0">
        <property type="entry name" value="ACETOLACTATE SYNTHASE SMALL SUBUNIT 1, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22629">
        <property type="entry name" value="ACT_AHAS_ss"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10369">
        <property type="entry name" value="ALS_ss_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="4"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU368092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="166" mass="18373" checksum="2D3D416A1EA3F988" modified="2013-06-26" version="1">MKHVISALVLNKPGVLQRISGLFSRRGFNISSITVGTTENPNISRMTIVVNGDDKVLEQVVKQLNKLIDVIKVSELQEKKSVQRELCLIKIYAPTESAKSQVIQYTNIFRGNIVDISHESLIVEITGNEDKINAFIDLVKPLGIKEIARTGITALTRGPKVLKPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPI7</accession>
    <name>N6VPI7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Dimethylmenaquinone methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05683</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95805.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592788.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPI7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01266"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1108"/>
    <dbReference type="OrthoDB" id="15246at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047443">
        <property type="term" value="F:4-hydroxy-4-methyl-2-oxoglutarate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008948">
        <property type="term" value="F:oxaloacetate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16841">
        <property type="entry name" value="RraA_family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.40">
        <property type="entry name" value="Ribonuclease E inhibitor RraA/RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005493">
        <property type="entry name" value="RraA/RraA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036704">
        <property type="entry name" value="RraA/RraA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33254">
        <property type="entry name" value="4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33254:SF4">
        <property type="entry name" value="4-HYDROXY-4-METHYL-2-OXOGLUTARATE ALDOLASE 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03737">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89562">
        <property type="entry name" value="RraA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95805.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="186" mass="20718" checksum="4A5A95BDB4A303C2" modified="2013-06-26" version="1">MFSVPNLCDAGALYIKNLKPVLNNQKVIIGEVVTVRTNDDWGCVVKAISYAKGKIIFVKNEGEKAVWGGLASLNAKIKGVKAVVIYGAIRDIEDIISLKFPVFYKEVNAEAGKPLNVGEINIPIDIYNITVSPGDYIACDMNGVCLIKRRCYNEIMERVKKVKSKENKIKERIMKGQDLSEILNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPJ8</accession>
    <name>N6VPJ8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH ubiquinone oxidoreductase 20 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_05773</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592815.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPJ8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01251"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1125"/>
    <dbReference type="OrthoDB" id="5740at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008137">
        <property type="term" value="F:NADH dehydrogenase (ubiquinone) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052375">
        <property type="entry name" value="Complex_I_20kDa-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006138">
        <property type="entry name" value="NADH_UQ_OxRdtase_20Kd_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005012">
        <property type="entry name" value="PRK06411.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42989:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42989">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01150">
        <property type="entry name" value="COMPLEX1_20K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="7">
        <location>
            <begin position="20"/>
            <end position="129"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="142" mass="15643" checksum="52EBBBA1CBA15700" modified="2013-06-26" version="1">MKNLVRKRSVHLCVINTGGCNGCDVEILSCICQRYDIEQYGIYFHNNPREADILVITGPVTLPWKERLKEIYEKTPEPKIVVAVGACALTGGIFKEGHVVGGVDKVIPVDAKVPGCPPRPSEIVEALLKVIPLIALKEKRLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPT7</accession>
    <name>N6VPT7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system Cms protein Csm2</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">CRISPR type III A-associated protein Csm2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_05274</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">This subunit may be involved in monitoring complementarity of crRNA and target RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Csm2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000035">
        <property type="protein sequence ID" value="ENN95915.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592279.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPT7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00742"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1030"/>
    <dbReference type="OrthoDB" id="65911at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09647">
        <property type="entry name" value="Csm2_III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010149">
        <property type="entry name" value="CRISPR-assoc_prot_Csm2_III-A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01870">
        <property type="entry name" value="cas_TM1810_Csm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03750">
        <property type="entry name" value="Csm2_III-A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0694">RNA-binding</keyword>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="10"/>
            <end position="21"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00016118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00031723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95915.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="150" mass="17696" checksum="BF81FB852E0EA9F6" modified="2013-06-26" version="1">MGYSRYQNLKGERGEGNRKKEEKEIVLNDKEIDIILNITSENANKMVEIAEKFANEILSIPNTKIREFYDYVLKIKENEDWYKKLVLLKPKMAYTYGKETNKGKKIALKKLHDTFSEIIDRIDNLNKFNNFKTFFEAVIAYHKLHGGKEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPW4</accession>
    <name>N6VPW4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA repair protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_04840</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95940.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592051.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPW4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00522"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.948"/>
    <dbReference type="OrthoDB" id="145577at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020209">
        <property type="entry name" value="Cas6b_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041528">
        <property type="entry name" value="Cas6b_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17262">
        <property type="entry name" value="Cas6b_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17955">
        <property type="entry name" value="Cas6b_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cas6b N-terminal" evidence="2">
        <location>
            <begin position="1"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="Cas6b C-terminal" evidence="1">
        <location>
            <begin position="108"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF17262"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF17955"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95940.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="218" mass="25583" checksum="E2562F4C38AC7685" modified="2013-06-26" version="1">MKLPMIICRLKTNKPLKKIHTPYLRGYILKKFPNYVELHHHSNGGFLYMYPKIQYKVINGDAVILGIKEGINILKDIIFDIDKLELNKEVYYVVNGYIKAVFEEFGESEEMITYKFISPWIALNEKNYLKYKNMDNESRKELLEKILIGNILSMSKYLNYTVKEKLEAEILRLENLTVKYKGNKFIGFFGEFKVNFNIPNYLGIGRKVSKGFGTVIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ14</accession>
    <name>N6VQ14_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2-isopropylmalate synthase</fullName>
            <ecNumber evidence="7">2.3.3.13</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Alpha-IPM synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Alpha-isopropylmalate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">J422_05040</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>3-methyl-2-oxobutanoate + acetyl-CoA + H2O = (2S)-2-isopropylmalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21524"/>
            <dbReference type="ChEBI" id="CHEBI:1178"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 14">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.13" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95980.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592133.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ14"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00481"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.988"/>
    <dbReference type="OrthoDB" id="6555at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019298">
        <property type="term" value="P:coenzyme B biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07940">
        <property type="entry name" value="DRE_TIM_IPMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.260:FF:000001">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000010">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.270:FF:000003">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050073">
        <property type="entry name" value="2-IPM_HCS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002085">
        <property type="entry name" value="PRK00915.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002086">
        <property type="entry name" value="PRK00915.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277:SF9">
        <property type="entry name" value="2-ISOPROPYLMALATE SYNTHASE 1, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10277">
        <property type="entry name" value="HOMOCITRATE SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="16" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0432">Leucine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 14 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="15">
        <location>
            <begin position="24"/>
            <end position="274"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00003715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00012973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022430"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN95980.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="511" mass="56101" checksum="77EDDB969BE1389F" modified="2013-06-26" version="1">MIIYREENEIIREALKNINLPKNVYIFDTTLRDGEQTPGVSLTVDEKVEIAVKLDELGVDVIEAGFPISSLGEREAIKKICSLNLNAEICGLARARKEDIDAAIDCGVDRIHTFIATSPLHRKYKLKMDKEEIIERAIEAIEYIKEHGIKVEFSAEDATRTELNYLKEVYKKAIEAGADIINVPDTVGVMIPRAMNYLIKYLNDLDVTISVHCHNDFGLAVANSLAAVEAGAKQIHCTINGLGERGGNAALEEVVMSLHCIYGIKTNIKTEKLYEVSKLVAKYTEINVQPNKAIVGENAFAHESGIHAHGVLAHALTYEPIPPELVGQKRKIILGKHTGTHAIKAKLKELGYNNLTEEQFKEIVKRIKSLGDKGKRITDRDVEAIVEDVLGRVAKKERVVDLEQIAVMTGNIIPTASVALRINKDVKKVSSIGVGPVDAAIKAIQKAIGEKIKLKEYHLSAITGGTDALAEVVVKLESDGKEVITKAASEDIVRASVEAVIDGINRLLKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQC2</accession>
    <name>N6VQC2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Glutamate--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Glutamyl-tRNA synthetase</fullName>
            <shortName evidence="10">GluRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 16" type="primary">gltX</name>
        <name evidence="16" type="ORF">J422_04318</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>tRNA(Glu) + L-glutamate + ATP = L-glutamyl-tRNA(Glu) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9663"/>
            <dbReference type="Rhea" id="RHEA-COMP:9680"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.17"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.17" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96065.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591758.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQC2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00660"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.846"/>
    <dbReference type="OrthoDB" id="10470at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004818">
        <property type="term" value="F:glutamate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043604">
        <property type="term" value="P:amide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006424">
        <property type="term" value="P:glutamyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09287">
        <property type="entry name" value="GluRS_non_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.240.10:FF:000033">
        <property type="entry name" value="Glutamate--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.240.10">
        <property type="entry name" value="Ribosomal Protein L25, Chain P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02076">
        <property type="entry name" value="Glu_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050132">
        <property type="entry name" value="Gln/Glu-tRNA_Ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004526">
        <property type="entry name" value="Glu-tRNA-synth_arc/euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000924">
        <property type="entry name" value="Glu/Gln-tRNA-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020058">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_cat-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020059">
        <property type="entry name" value="Glu/Gln-tRNA-synth_Ib_codon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020056">
        <property type="entry name" value="Rbsml_bL25/Gln-tRNA_synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011035">
        <property type="entry name" value="Ribosomal_bL25/Gln-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049437">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00463">
        <property type="entry name" value="gltX_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003169">
        <property type="entry name" value="PRK04156.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097:SF5">
        <property type="entry name" value="GLUTAMATE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43097">
        <property type="entry name" value="GLUTAMINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00749">
        <property type="entry name" value="tRNA-synt_1c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03950">
        <property type="entry name" value="tRNA-synt_1c_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20974">
        <property type="entry name" value="tRNA-synt_1c_C2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00987">
        <property type="entry name" value="TRNASYNTHGLU"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50715">
        <property type="entry name" value="Ribosomal protein L25-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib catalytic" evidence="13">
        <location>
            <begin position="81"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="domain" description="Glutamyl/glutaminyl-tRNA synthetase class Ib anti-codon binding" evidence="14">
        <location>
            <begin position="383"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA synthetases class I (E and Q) anti-codon binding" evidence="15">
        <location>
            <begin position="477"/>
            <end position="525"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="46"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="10">
        <location>
            <begin position="88"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048351"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU363037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF03950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF20974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN96065.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="539" mass="63670" checksum="11880E555832D089" modified="2013-06-26" version="1">MENKIREIAIKNAAKYGKANVKSVLGAFLSEFPEYRSKVKDILPIVEKIVNDINNQKINIKIEENKKRKDKELELDNVKDKVVLRFAPNPSGPLHIGHARAAVLNDYFAKKYNGKFILRLEDTDPKRVLPEAYDMIVEDLEWLGVKIDEIVIQSDRLDIYYKYGEMLIKDGKAYVCECEAEKFRELRNRGIACSCRDREIEDNLYLWEKMLNGEIKAVVRLKTDLKHKNPSIRDFPIFRVEKEKHPRVDAYVYPLMNFSVPVDDHLLGITHVLRGKDHIVNTEKQRYIYKYFNWEEPEFIHYGILKIEDSVLSTSQIYEGIKKGIYEGWDDVRLGTLRALKRRGIRPEAIYKIMLNIGLKKADIKFSWENLYAINRELIDKEAKRYFFVWKPKLMKIEGAEKRIVRLRKHPDVKEFGYRELVFDGEVYVVSSEVEEGRMYRLMELFNVVVEKIEDNFIKARYHSDDFKVARKNRAKIIHWVPKDKVDVVILTPNGVIEGFAEYNVKEINVDEIIQFERFGFARLDKKEENKLIFCYAHR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQI1</accession>
    <name>N6VQI1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Methionine--tRNA ligase</fullName>
            <ecNumber evidence="15">6.1.1.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="15">Methionyl-tRNA synthetase</fullName>
            <shortName evidence="15">MetRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15 19" type="primary">metG</name>
        <name evidence="19" type="ORF">J422_04018</name>
    </gene>
    <organism evidence="19 20">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="19 20" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="19 20">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 15">Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>tRNA(Met) + L-methionine + ATP = L-methionyl-tRNA(Met) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:13481"/>
            <dbReference type="Rhea" id="RHEA-COMP:9667"/>
            <dbReference type="Rhea" id="RHEA-COMP:9698"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78530"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="15">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="15">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 15">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="15">Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="19">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.10" evidence="15"/>
    <dbReference type="EMBL" id="APMM01000025">
        <property type="protein sequence ID" value="ENN96135.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591459.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQI1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00734"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.787"/>
    <dbReference type="OrthoDB" id="371856at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004825">
        <property type="term" value="F:methionine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006431">
        <property type="term" value="P:methionyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07957">
        <property type="entry name" value="Anticodon_Ia_Met"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00814">
        <property type="entry name" value="MetRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02800">
        <property type="entry name" value="tRNA_bind_EcMetRS_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.20:FF:000001">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000042">
        <property type="entry name" value="Methionine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.20">
        <property type="entry name" value="Methionyl-tRNA synthetase, Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00098">
        <property type="entry name" value="Met_tRNA_synth_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041872">
        <property type="entry name" value="Anticodon_Met"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004495">
        <property type="entry name" value="Met-tRNA-synth_bsu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023458">
        <property type="entry name" value="Met-tRNA_ligase_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014758">
        <property type="entry name" value="Met-tRNA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015413">
        <property type="entry name" value="Methionyl/Leucyl_tRNA_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033911">
        <property type="entry name" value="MetRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029038">
        <property type="entry name" value="MetRS_Zn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002547">
        <property type="entry name" value="tRNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00398">
        <property type="entry name" value="metG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00399">
        <property type="entry name" value="metG_C_term"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001100">
        <property type="entry name" value="PRK00133.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765">
        <property type="entry name" value="METHIONINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45765:SF1">
        <property type="entry name" value="METHIONINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19303">
        <property type="entry name" value="Anticodon_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09334">
        <property type="entry name" value="tRNA-synt_1g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01588">
        <property type="entry name" value="tRNA_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01041">
        <property type="entry name" value="TRNASYNTHMET"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57770">
        <property type="entry name" value="Methionyl-tRNA synthetase (MetRS), Zn-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50886">
        <property type="entry name" value="TRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15 17" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="10 15 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 15 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8 15 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 15 17" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="20" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15 16" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 15 16" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="9 15" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="TRNA-binding" evidence="18">
        <location>
            <begin position="537"/>
            <end position="637"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="15">
        <location>
            <begin position="10"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="15">
        <location>
            <begin position="318"/>
            <end position="322"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="142"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="145"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="157"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="321"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00047364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="RuleBase" id="RU363039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="EMBL" id="ENN96135.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="637" mass="74344" checksum="7059FFB395C2C8B6" modified="2013-06-26" version="1">MKYLITTALAYTNGPLHLGHVRSTYIPADILYKYLKLKGEEVIHVGGTDNHGVPILLIAEKEGKNPEEIVEKYHNEIKRDLESLNIEFDVFGKTHSDIHIKTAQEFFLKLKENGYIYEKEIEQFYCENCKKFLPDRYVEGTCPYCGGEARGDHCESCGRHIEPIELKDPYCVICGNRPVIKKTIHYFFKLSALKEELERYIKNAEEMPEHVRNLALNWINELRDWDVSRDISWGVPIPGSDQVMYVWLEAPIGYISFTKMINKENYWVDKDTKIYHFIGKDIIVHHAVFWPGMLIAHKGYNLPTSVIAGGYLTLEGKKMSTSKRWVIWVKDFVEVFEPDYLRYYLIIASPLFKDSDFSFEEFKNKINNELINIIGNFTHRVLTFTYRKFKSVPVVDSLEDEDKKILDMIEKTLDEYDKNIRSFKFREALVNVLHLAIEGNSYFQKMEPWNIESEDRLKQILYVCCKLVKTIAYLLYPYMPKKSKEILDIMNEELDIEIRGNELKKPKIVFKKVEDDDIERLKNKLKGDNMEFIDIQDFQKIDLRVGKIVEAEEVKGSKKLLRLTIDLGNEKRQIVSGIKEYYKPEELINKKVIVICNLKPAKIFGITSEGMILAAEDDKTVSLLTVDRDVKEGSKVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQI6</accession>
    <name>N6VQI6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">HTH lysR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_03738</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the LysR transcriptional regulatory family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96140.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591329.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQI6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00380"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.730"/>
    <dbReference type="OrthoDB" id="62169at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000976">
        <property type="term" value="F:transcription cis-regulatory region binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000847">
        <property type="entry name" value="LysR_HTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30126">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30126:SF40">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR GLTR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00126">
        <property type="entry name" value="HTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50931">
        <property type="entry name" value="HTH_LYSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH lysR-type" evidence="5">
        <location>
            <begin position="14"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96140.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="258" mass="30267" checksum="E489EB45D052B906" modified="2013-06-26" version="1">MKADIILKDKDKFVTSNQILLLLALYKTKSQNSAAKLLNISPSSFNIQLKRLEKKLGFKLYYSSPNGTVLTEKAINLLEEYLIKKSRLESERFTVSGYISGELAKKLFDDPIITSFNNAIKLLKMDLVDILGLDDSYWLYRLEDDRFLKSEVGNKKIDIIHTYEDHFVMVYKEEFNYKNLIGIRYSPHRILYNILKSNNINFRVRIRVNNPFKAIELVNKGYSLFLNESLIKYAEGLNIEYPKFYEKTIHTITFIKLY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQK4</accession>
    <name>N6VQK4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03908</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96170.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591389.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQK4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00412"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.766"/>
    <dbReference type="OrthoDB" id="64172at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043941">
        <property type="entry name" value="EMC6-arch"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19094">
        <property type="entry name" value="EMC6_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="66"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96170.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="91" mass="10280" checksum="54925998DF59236B" modified="2013-06-26" version="1">MDIEGKCCTIHTFGGIIFGYLANYIYLMGLGIFSGIVTLLFLFFGAVIFGHITAKIFGEKCLNQKQWFSCGLLPFYLVAIIVWILKYNNII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQN8</accession>
    <name>N6VQN8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker protein 17</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03658</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96210.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591270.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQN8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01373"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.714"/>
    <dbReference type="OrthoDB" id="52971at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016762">
        <property type="entry name" value="Methan_mark_17"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03291">
        <property type="entry name" value="methan_mark_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09886">
        <property type="entry name" value="DUF2113"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019464">
        <property type="entry name" value="UCP019464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96210.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="184" mass="21574" checksum="4A484ED3CA470C99" modified="2013-06-26" version="1">MAEIYVDCEDEAGKKIYTKVIQTALEDLVLGKSIVRVEFIAREKEPYFILAVLPKQTRKKIKVRDVAEIVEEKKVDGKKLYKLKIVDETYAPHLLKNIKVLDQPSRFEIITDEVDLDMDIYDTSKEFIDKIMDFMNRVFPEGMRIRKTFIDKAIVSIASERPLKEEEIKKTLEVKEKLESYKII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQQ0</accession>
    <name>N6VQQ0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Membrane protein 6-pyruvoyl-tetrahydropterin synthase-related domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03473</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000022">
        <property type="protein sequence ID" value="ENN96225.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591141.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQQ0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01339"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.677"/>
    <dbReference type="OrthoDB" id="41895at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02795">
        <property type="entry name" value="CBM6-CBM35-CBM36_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.260">
        <property type="entry name" value="Galactose-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="23"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="108"/>
            <end position="128"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="140"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="195"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="240"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="306"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="335"/>
            <end position="362"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="382"/>
            <end position="402"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="414"/>
            <end position="435"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="1030"/>
            <end position="1051"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96225.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="1075" mass="126576" checksum="5062B478EEABE0CA" modified="2013-06-26" version="1">MKGLNNILRMCVTLFKKKELKEFLVVFLVFMILFLVPTFKLFIKDGYIILSEHLEAISYEKFESIFKYSWDYQIGIFPFAEFPKYYLYSLLVFIGKSIHLNFKYFEILLLGLQFPLGFISAYILNRYIVRKILNIQINSLEILMLIVASLIYVINPWVASYQHNISLKFQHALLPLIFLYFLKTIQEEKNTIKNSIIGAFLLSLVASYRYTIVILPLFILTICVYLILNKKSIKKLFIKISAYTFFAYMTMLGKVITPVLHALYTQKVKWAEEFTASYLHLFPLINVFSLKMETYLSIFLVYCDNYIYLFLPVTIVSFLYLLIWRKKITKQNWIYFLPIIIFITYIPLCVKEISLFSLDFIWKIPFSDDILRLLRSGRINAMPILLALSISTLLTLFSFSKTLLLIRNKKLQKVLLYSLTLMIIIFSSISAWPIFTGNMNNYWEPAKIPKDYYIANKILSNFSEDENYALWMPPFSCSKPIWSNVSDTPAGIFDWRNSELPVYSYREMYFFDYYNPLHSFPFVRALDGYKGLDWDKIFYPLNIRFIVVHYDLDNPYVSNINHIIKNKLLKINQELNKIYEGNYLAVYKVFDEKVSKVRITTPILLDDSLKIYGSIVNVLDFKTLNISSMVFYNPFHSTLNISKVNSIVVNSNLLKAIFSYYTPSIIKIQTDKRLEDPSKNWIFSPNPRYKFQTYIKKSSIYEWSWQSDYGYGLVFTWAKNTKLTISFKADKPDKYKLFIRYFKNQKGGKIAVYLDGKPITIKTKDQLNKFVWEDLGTLYLEKGEHKIVLENIEGFNAVNLFALIPEKEYYKAKKEVEKLLQNKTIIYVFEGESDLYYSNAKIIKDYNTSNGEELLFNKNGKAWQDVEIVKNGTYRIALRGEGIFKVKIGTKEFISKPNNNFIYTPPFNLTEGKYKLEIIPLKEKSKLDVIWLYSSNKTLKDLFEVKEKPAKIISYEKINPTLWKVKVNATKPFMLSFAEAYDPLWEARVYKDGKLVEKVKSIPLYSVINGFWINETGNLDIVIRYTPQDWFDIGLAISGITFFCCLAYLFYDWRREDKWAKNLEGKIKNLIKFRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQR8</accession>
    <name>N6VQR8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Phosphate ABC transporter ATP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_03243</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell inner membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96245.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590941.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQR8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01036"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.635"/>
    <dbReference type="OrthoDB" id="31298at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03260">
        <property type="entry name" value="ABC_PstB_phosphate_transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000132">
        <property type="entry name" value="Phosphate import ATP-binding protein PstB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005670">
        <property type="entry name" value="PstB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00972">
        <property type="entry name" value="3a0107s01c2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43423:SF1">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="9">
        <location>
            <begin position="6"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96245.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="252" mass="28990" checksum="D25DA46031948AB8" modified="2013-06-26" version="1">MEKIKMETKNLNLWYGEKQALFDISLPIYENRITALIGPSGCGKSTFLRCLNRLNDLIENVRIEGKVFLDGKNIYDKDVDVYELRRRVGMVFQKPNPFAMSIYDNVAFGPRIHGIKNKKELDKIVEWALKKAALWDEVKDELHKNALKLSGGQQQRLCIARAIAVKPEVLLMDEPTSALDPISTLKIEELITELAKDYTVVIVTHNMQQASRISDYTAFFLMGKLIEFDKTEEIFLNPKKKETDDYIRGRFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR05</accession>
    <name>N6VR05_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0288 protein J422_02604</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02604</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0288 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96335.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590549.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR05"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00073"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.510"/>
    <dbReference type="OrthoDB" id="140355at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01089">
        <property type="entry name" value="UPF0288"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058492">
        <property type="entry name" value="DUF8179"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016466">
        <property type="entry name" value="Methan_mark_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03268">
        <property type="entry name" value="methan_mark_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26548">
        <property type="entry name" value="DUF8179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005852">
        <property type="entry name" value="UCP005852"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54184">
        <property type="entry name" value="Penicillin-binding protein 2x (pbp-2x), c-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative peptidyl-prolyl cis-trans isomerase" evidence="2">
        <location>
            <begin position="366"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01089"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26548"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96335.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="494" mass="56286" checksum="483CCDCF64E2E81E" modified="2013-06-26" version="1">MAKVIVNGKEKEGEFLKDVIKDEFYKDGANIVIIKGIKKSAETTRKFLIKTTKGNITIAITENNEAAEFFLNNYKSFAKKLKWVSGVDVAFGSVRIDLPISTESQKFNKWDVLLSLSGFDVEEGHLVFIKKKTEGIYGLKNPKIGRVVGGKWVIDRLKLNDRIIDIEPITEEKESVDYVVTNDLNIKLEDGWKIYSYFIAEFDGKPSAVEHCLALMEDNIFEISEYTNTYIADCRLQTLKIEESELKDRDRGVITVRNFGVGEGKVYIYKESRTSSPSHTVVGRVKEGFELINFSNSGFLTVKTIPERLCAIGMDVNKAEEMFNKYDIDIEVEGKGIVVDQQPEYTLDVLKEKKVKLKAIDDKKIISIELYEDKAPISTWYFRKVSGLTTKRVGKLKVYFKHKDVIMFKGLQEYAKGLLPENTPKDKVEANSIGISNMVSRYKGMIGIRLEENDKYGPTGEGFDKTNIVGKVIENVEHLKKVKMNDEVYFLRKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR76</accession>
    <name>N6VR76_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Nmd3 N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06728</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000058">
        <property type="protein sequence ID" value="ENN95641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593985.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000058.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR76"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01360"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1309"/>
    <dbReference type="OrthoDB" id="15051at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043023">
        <property type="term" value="F:ribosomal large subunit binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039768">
        <property type="entry name" value="Nmd3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007064">
        <property type="entry name" value="Nmd3_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746:SF2">
        <property type="entry name" value="60S RIBOSOMAL EXPORT PROTEIN NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12746">
        <property type="entry name" value="NONSENSE-MEDIATED MRNA DECAY PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04981">
        <property type="entry name" value="NMD3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nmd3 N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="361" mass="42358" checksum="1AB45B6CB8612CD3" modified="2013-06-26" version="1">MRGICYRCGAEDELIEGLCPLCYAQEHPLFEIDNIIQIEVCHMCGSYKRKVWQTPKSEDPYEIVEEIVYYAIKDNIKKKGEIELEIIPKISQLPGGKRSKLIIPVEIIAYGKLPKEKEKREYRKEVEVHLNMVQCPRCSRYMSNYYEATLQVRAMNRYLTEKEKEELDKFVREEVYKRLKKDRMAFISKFIPQKEGLDYQLGSIGAARYLAHRIKEKYGGKITESSTLVGVDSTGKELHRLTISVRIPEYRVGDVVKYRDKYYKVSSITEHKVYLKSLDKDDKLGLSWHVAEKETKLEKKWEELSRGTVIATTPNLMVMDDENYEIYEFDKKEGIKEGDKVRLLKKDIVYFVEKEGENGDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRG0</accession>
    <name>N6VRG0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Putative arsenical pump-driving ATPase</fullName>
            <ecNumber evidence="8">7.3.2.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Arsenical resistance ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Arsenite-translocating ATPase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Arsenite-transporting ATPase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">J422_06080</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Anion-transporting ATPase. Catalyzes the extrusion of arsenite.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>arsenite(in) + ATP + H2O = arsenite(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11348"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29242"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the arsA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.7" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95746.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593233.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRG0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00535"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1184"/>
    <dbReference type="OrthoDB" id="46198at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015446">
        <property type="term" value="F:ATPase-coupled arsenite transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd02035">
        <property type="entry name" value="ArsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001801">
        <property type="entry name" value="Putative arsenical pump-driving ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025723">
        <property type="entry name" value="ArsA/GET3_ATPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016300">
        <property type="entry name" value="ATPase_ArsA/GET3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00345">
        <property type="entry name" value="GET3_arsA_TRC40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803">
        <property type="entry name" value="ARSENICAL PUMP-DRIVING ATPASE ARSENITE-TRANSLOCATING ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10803:SF3">
        <property type="entry name" value="ATPASE GET3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02374">
        <property type="entry name" value="ArsA_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0059">Arsenical resistance</keyword>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <feature type="domain" description="ArsA/GET3 Anion-transporting ATPase-like" evidence="13">
        <location>
            <begin position="19"/>
            <end position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00052296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00059736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066752"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00074681"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00075317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00078871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00080604"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02374"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN95746.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="327" mass="37520" checksum="B391254B6AF28427" modified="2013-06-26" version="1">MISKIKNSLKALTENKGETKYILFGGKGGVGKTTMSAATGIYLAEQGYKVCIVSTDPAHSLRDIFEQEFSHEPTKVKGYDNLYVVEIDPEKAMEEYKEKLKAQIEENPLLGEMLEEQLEIASLSPGTDESAAFDTFLKYMESNEFDYIIFDTAPTGHTLRFLSMPEVMDKYLTRMIKLKKQISGFMKMFKKFLPFSSSDNDIDYDKMLKELEEMKKRIEKAKKILSDPEKTSFRLVVIPEEMSILESERAMKALQKYNINVDAVIVNQLIPENVSCEFCRARRELQLKRLEMIKEKFGDKVIAYVPLLKKEAKGIETLKEISKILYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRP6</accession>
    <name>N6VRP6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flagellar accessory protein FlaH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_05828</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95831.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592836.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRP6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01240"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1136"/>
    <dbReference type="OrthoDB" id="63735at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19475">
        <property type="entry name" value="FlaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056568">
        <property type="entry name" value="ArlH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006320">
        <property type="entry name" value="PRK08533.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF3">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN H-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="3">
        <location>
            <begin position="23"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95831.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="230" mass="25741" checksum="0473A43656161ACA" modified="2013-06-26" version="1">MQIAKIELARDDLDKRIGGGIPFNSLVIIEGEESTGKSVLCQRLAYGFLQNRHSVTYVSTQLTTTEFIKQMMSLEYNINKKLLSGALLYIPVYPLIADNKRRDGFLKKIMETKAFYEKDVIIFDSLSSLIANDASDVNVNDLMAFFKRIVALKKIIICTINPKELSEEVLTMIRTSSTMLIKTDIITFAGSIKNAAKILKYNMAPGTYQKTIVFRVEPKIGLAVEIASVA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRW0</accession>
    <name>N6VRW0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01328</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589929.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRW0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00023"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.260"/>
    <dbReference type="OrthoDB" id="62339at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="131" mass="15204" checksum="59A9C92C5A475813" modified="2013-06-26" version="1">MKKIIVKNKKEFLNNAKEYGQEDEIYINLELDRDVAIALLENIDPKKIYLPESKYKRTSKKIIKALNGVGVEVIAVKPKTGRPTNIDEIIKKYLHLKPKEIAEKTGINLKTVEYHYYKLKRAVKCSDTKKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRZ7</accession>
    <name>N6VRZ7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hypoxanthine/guanine phosphoribosyltransferase</fullName>
            <shortName evidence="5">HGPRTase</shortName>
            <ecNumber evidence="5">2.4.2.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hpt</name>
        <name evidence="7" type="ORF">J422_01111</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes a salvage reaction resulting in the formation of IMP that is energically less costly than de novo synthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25424"/>
            <dbReference type="ChEBI" id="CHEBI:16235"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58115"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:17973"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="2.4.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Purine metabolism; IMP biosynthesis via salvage pathway; IMP from hypoxanthine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the purine/pyrimidine phosphoribosyltransferase family. Archaeal HPRT subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.8" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589890.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRZ7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00820"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.217"/>
    <dbReference type="OrthoDB" id="8323at2157"/>
    <dbReference type="UniPathway" id="UPA00591">
        <property type="reaction ID" value="UER00648"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052657">
        <property type="term" value="F:guanine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004422">
        <property type="term" value="F:hypoxanthine phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032264">
        <property type="term" value="P:IMP salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01467">
        <property type="entry name" value="Hypx_phosphoribosyltr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026597">
        <property type="entry name" value="HGPRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050118">
        <property type="entry name" value="Pur/Pyrimidine_PRTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040646">
        <property type="entry name" value="HPT_Archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002635">
        <property type="entry name" value="PRK02304.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43864">
        <property type="entry name" value="HYPOXANTHINE/GUANINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43864:SF1">
        <property type="entry name" value="XANTHINE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 5 7" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4 5" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="6">
        <location>
            <begin position="51"/>
            <end position="161"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022726"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="183" mass="20352" checksum="DBE8C5C613AF1116" modified="2013-06-26" version="1">MLLEETLKSCPIVKRGNYDYFIHPITDGVPVVKPELLREVAVRILKVGNFDEVDKIVTAEAMGIHLATILSIYTDIPFVIMRKRQYNLPNEIPVFQKTGYSKGQLYLNGINKGDKVVIVDDVISTGGTMMAIISALEKAGAEIKDIICVIERGNGKEIVEKKTGYKIKTLVKVDVVDGKLIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS20</accession>
    <name>N6VS20_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Beta-lactamase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01016</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000006">
        <property type="protein sequence ID" value="ENN96675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589871.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS20"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01015"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.198"/>
    <dbReference type="OrthoDB" id="40950at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43694">
        <property type="entry name" value="RIBONUCLEASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43694:SF1">
        <property type="entry name" value="RIBONUCLEASE J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="82"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="domain" description="Zn-dependent metallo-hydrolase RNA specificity" evidence="2">
        <location>
            <begin position="459"/>
            <end position="488"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF07521"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="511" mass="59310" checksum="E602B1147ABCA3FF" modified="2013-06-26" version="1">MKITIYDGANTIGGNKIYIKEGYNGIFLDFGMNFANYSKYYEEYLSERSARGIYDLWHLNLIPKLNIYRSDLIPKDLGIYRYPKIPINAVLISHAHLDHVGNVAILNEEIPMVGSSITMTILKALRDTSTGKHLGIDLPYYSIKKPKDEKGYVLESDRKSPYCSRKIILTDDNKNIEEFIFYRPGQNSSNKVKKINQNEVKMLYEEDFGFEIKAFEVDHSIYGAVGYIVEGDVSVAYTGDFREHGKNKDKTKKFIKEAKNASVLITEGTRVNRDNDVNVSEEDVYNNSLKIIENAKGLVIADFSPRNFERLEIFKEIAKKTGRELVITTKDAYFLWALKEVDKIDIIDDDVKIYENLKATQYKWEEEILDIYCNYIISPFEIRKNCENYILCFSFYDMPHLLDVNPDGGIYIYSSSEAFGEEQEFSFLRLWNWLKHFNFEIYGFKVDENGKPIFEKGFHTSGHISKEGLRRVIEKIDPDYIIPVHTENPELFRWAFGERVVLLNNGESLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS28</accession>
    <name>N6VS28_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit Rpo7</fullName>
            <ecNumber evidence="4">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">DNA-directed RNA polymerase subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpo7</name>
        <name evidence="4" type="synonym">rpoE</name>
        <name evidence="6" type="ORF">J422_00901</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the RNA polymerase complex. Forms a stalk with Rpo4 that extends from the main structure.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="4">Forms 2 domains with an elongated structure; Rpo4 packs into the hinge region between the 2 domains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS28"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00594"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.177"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04331">
        <property type="entry name" value="RNAP_E_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04460">
        <property type="entry name" value="S1_RpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.120:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerase II subunit RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.120">
        <property type="entry name" value="RNA polymerase Rpb7-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00865">
        <property type="entry name" value="RNApol_arch_Rpo7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036898">
        <property type="entry name" value="RNA_pol_Rpb7-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004519">
        <property type="entry name" value="RNAP_E/RPC8"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046399">
        <property type="entry name" value="RNApol_Rpo7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045113">
        <property type="entry name" value="Rpb7-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005576">
        <property type="entry name" value="Rpb7-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006333">
        <property type="entry name" value="PRK08563.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00448">
        <property type="entry name" value="rpoE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709:SF4">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II SUBUNIT RPB7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12709">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE II, III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03876">
        <property type="entry name" value="SHS2_Rpb7-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88798">
        <property type="entry name" value="N-terminal, heterodimerisation domain of RBP7 (RpoE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 4 6" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="4 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="S1 motif" evidence="5">
        <location>
            <begin position="82"/>
            <end position="166"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00865"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="172" mass="19490" checksum="5D324EA594E388E9" modified="2013-06-26" version="1">MYKILEIMDVVRVPPDEFGKDLKDTIKKILIEKYEGRLDKDIGFILAITDVKKIGEGKVVHGDGSAYHPVIFETLVYMPEMYELIEGEAVDVVEFGCFVRLGPLDGLIHVSQIMDDYVVYDPKRQAIIGKETGKVLEIGDYVRARIVAISLKSERKRGSKIALTMRQPYLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS52</accession>
    <name>N6VS52_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Hydroxymethylglutaryl-CoA synthase</fullName>
            <shortName evidence="4">HMG-CoA synthase</shortName>
            <shortName evidence="4">HMGCS</shortName>
            <ecNumber evidence="4">2.3.3.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_05045</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Catalyzes the condensation of acetyl-CoA with acetoacetyl-CoA to form 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA). Functions in the mevalonate (MVA) pathway leading to isopentenyl diphosphate (IPP), a key precursor for the biosynthesis of isoprenoid compounds that are building blocks of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>acetoacetyl-CoA + acetyl-CoA + H2O = (3S)-3-hydroxy-3-methylglutaryl-CoA + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10188"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
            <dbReference type="ChEBI" id="CHEBI:57286"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="4">Metabolic intermediate biosynthesis; (R)-mevalonate biosynthesis; (R)-mevalonate from acetyl-CoA: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Interacts with acetoacetyl-CoA thiolase that catalyzes the precedent step in the pathway and with a DUF35 protein. The acetoacetyl-CoA thiolase/HMG-CoA synthase complex channels the intermediate via a fused CoA-binding site, which allows for efficient coupling of the endergonic thiolase reaction with the exergonic HMGCS reaction.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the thiolase-like superfamily. Archaeal HMG-CoA synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.10" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95981.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592135.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS52"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00480"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.989"/>
    <dbReference type="OrthoDB" id="5812at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004421">
        <property type="term" value="F:hydroxymethylglutaryl-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010142">
        <property type="term" value="P:farnesyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019287">
        <property type="term" value="P:isopentenyl diphosphate biosynthetic process, mevalonate pathway"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00827">
        <property type="entry name" value="init_cond_enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.47.10:FF:000046">
        <property type="entry name" value="UPF0219 protein M1627_1703"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01409">
        <property type="entry name" value="HMG_CoA_synth_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013747">
        <property type="entry name" value="ACP_syn_III_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004656">
        <property type="entry name" value="HMG_CoA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00748">
        <property type="entry name" value="HMG_CoA_syn_Arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003274">
        <property type="entry name" value="PRK04262.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323">
        <property type="entry name" value="3-HYDROXY-3-METHYLGLUTARYL COENZYME A SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43323:SF2">
        <property type="entry name" value="HYDROXYMETHYLGLUTARYL-COA SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08541">
        <property type="entry name" value="ACP_syn_III_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="2 4" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Beta-ketoacyl-[acyl-carrier-protein] synthase III C-terminal" evidence="5">
        <location>
            <begin position="218"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-thioester intermediate" evidence="4">
        <location>
            <position position="112"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor" evidence="4">
        <location>
            <position position="234"/>
        </location>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="112"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="153"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <note>ligand shared with acetoacetyl-CoA thiolase</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="201"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>CoA</name>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <note>ligand shared with acetoacetyl-CoA thiolase</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="243"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="4">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>(3S)-hydroxy-3-methylglutaryl-CoA</name>
            <dbReference type="ChEBI" id="CHEBI:43074"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF08541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95981.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="345" mass="37591" checksum="79905ABDD2E433B0" modified="2013-06-26" version="1">MAGIVGYGSYIPKYRIKVEEIARVWNKDADAIKKGLLVYEKSVPALDEDTATIAVEAARNALKRAEINGKEIEAIYVGSESHPYAVKPTATIVAEAIDATPNLTAADLEFACKAGTAGIQICMGLVDSGIIKYGLAIGADTAQSAPGDALEFTASAGGAAYIIGNKKEIAVFNGTFSYTTDTPDFWRREGRPYPKHGGRFTGEPAYFRHVLNAAKGLMEKLGTSPKDYDYCVFHQPNGKFYLRVAKILGFKEEQYKLGLLTPYIGNTYSGAVPLGLSNILDHCNGGERILAVSYGSGAGSDAFDITVKERINKVKDKAKKTAEYINNKEYIDYAIYIKFRKKIKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS91</accession>
    <name>N6VS91_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">phosphomevalonate dehydratase</fullName>
            <ecNumber evidence="7">4.2.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_00471</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Component of a hydro-lyase that catalyzes the dehydration of mevalonate 5-phosphate (MVA5P) to form trans-anhydromevalonate 5-phosphate (tAHMP). Involved in the archaeal mevalonate (MVA) pathway, which provides fundamental precursors for isoprenoid biosynthesis, such as isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>(R)-5-phosphomevalonate = (2E)-3-methyl-5-phosphooxypent-2-enoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:78975"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:229665"/>
            <dbReference type="EC" id="4.2.1.182"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="4">
            <dbReference type="Rhea" id="RHEA:78976"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer composed of a large subunit (PMDh-L) and a small subunit (PMDh-S).</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.182" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589776.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS91"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00046"/>
    <dbReference type="OrthoDB" id="18062at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01356">
        <property type="entry name" value="AcnX_swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.10">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012016">
        <property type="entry name" value="PMDh-S-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002840">
        <property type="entry name" value="PMDh-S-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020794">
        <property type="entry name" value="PMDh_S"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003046">
        <property type="entry name" value="PRK03955.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36577">
        <property type="entry name" value="DUF521 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G00490)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36577:SF3">
        <property type="entry name" value="DUF521 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G00490)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01989">
        <property type="entry name" value="AcnX_swivel_put"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004966">
        <property type="entry name" value="UCP004966"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphomevalonate dehydratase small subunit-like" evidence="8">
        <location>
            <begin position="24"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="110"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00045120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00046520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="110" mass="11687" checksum="8A45789F51EE520C" modified="2013-06-26" version="1" fragment="single">MELKGRAISKGRAEGVALVSKKPFSFLGGVDKDGKIIDKNSDVYGESLKGKIFVFPYGRGSTVGSYVIYSLAKKGILKGIVNKECEPIIASGAILAGIPLVDKVDIDKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSB3</accession>
    <name>N6VSB3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable GTP-binding protein EngB</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">engB</name>
        <name evidence="12" type="ORF">J422_04188</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Necessary for normal cell division and for the maintenance of normal septation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. EngB GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96041.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591711.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSB3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00684"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.819"/>
    <dbReference type="OrthoDB" id="65113at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01876">
        <property type="entry name" value="YihA_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00321">
        <property type="entry name" value="GTPase_EngB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030393">
        <property type="entry name" value="G_ENGB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019987">
        <property type="entry name" value="GTP-bd_ribosome_bio_YsxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003255">
        <property type="entry name" value="PRK04213.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11649:SF13">
        <property type="entry name" value="ENGB-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11649">
        <property type="entry name" value="MSS1/TRME-RELATED GTP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51706">
        <property type="entry name" value="G_ENGB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 10" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="3 10" id="KW-0132">Cell division</keyword>
    <keyword evidence="7 10" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10" id="KW-0717">Septation</keyword>
    <feature type="domain" description="EngB-type G" evidence="11">
        <location>
            <begin position="17"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009638"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023210"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00321"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96041.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="210" mass="24720" checksum="7AC4D32FF819E75F" modified="2013-06-26" version="1">MDFFEKYKILKQEKIKEKPKVIVVGRSNVGKSTLIRLLTGNKKVRVGKRPGVTLKISEYDMGGYILVDMPGFGYMAGIPKKVQEKIKDEIVHYIENNYKNIAAAVHIIDAKSFCEIVERWKDKEIPIDIEMFDFITELKISPILVVNKMDKIKKNKWNETLDKICSYFKCSPPWRQWKFIIPAILKRRYGLEEIKKMIYERVELFNKLRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSD4</accession>
    <name>N6VSD4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">MFS transporter family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00446</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589771.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSD4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00691"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.86"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17370">
        <property type="entry name" value="MFS_MJ1317_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518">
        <property type="entry name" value="C-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23518:SF2">
        <property type="entry name" value="MAJOR FACILITATOR SUPERFAMILY TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="167"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="232"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="244"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="277"/>
            <end position="294"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="337"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="2">
        <location>
            <begin position="12"/>
            <end position="393"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="393" mass="44402" checksum="C99EA5A000B847A0" modified="2013-06-26" version="1">MKKKKIFGIPKNVFLLGIVSFLNDMSSEMITSITPSYLIEVLNVDKLLSGTIMGAIESLSSLFKVIFGYISDKIRNRKVFVSFGYFLSTISKGALAFTHSWLDFLFLRVLDRLGKGIRTAPRDALIAESTKKTGKSFGFHRMMDTLGAVVGPILSIIILELLSNYSANFSYRMIFLLSFIPGILSVLLIIFFIKDTDKNLKKIIMGKSSLNSESLKLFLFVVAVASLGRYSYAFTIWKVYDVGYTVIQGLYFYIIFNIFYAITSYPIGTFSDKFDKRMFITLGFGIAALASLIFAFADSLWMLLLGFIFYSVYLAIEETIPRAYLAEIAEGFEKGTVIGAYHTVFGVFVFPASVIAGWLWQSYSLEYSFLYATVMNSLAMFLMILEWVLKWKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSE0</accession>
    <name>N6VSE0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Nitrogenase iron protein</fullName>
            <ecNumber evidence="14">1.18.6.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Nitrogenase Fe protein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Nitrogenase component II</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14">Nitrogenase reductase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">nifH</name>
        <name evidence="17" type="ORF">J422_04298</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 14">The key enzymatic reactions in nitrogen fixation are catalyzed by the nitrogenase complex, which has 2 components: the iron protein and the molybdenum-iron protein.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>N2 + 8 reduced [2Fe-2S]-[ferredoxin] + 16 ATP + 16 H2O = H2 + 8 oxidized [2Fe-2S]-[ferredoxin] + 2 NH4(+) + 16 ADP + 16 phosphate + 6 H(+)</text>
            <dbReference type="Rhea" id="RHEA:21448"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17997"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="1.18.6.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="14">Binds 1 [4Fe-4S] cluster per dimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 14">Homodimer.</text>
    </comment>
    <comment type="PTM">
        <text evidence="15">The reversible ADP-ribosylation of Arg inactivates the nitrogenase reductase and regulates nitrogenase activity.</text>
    </comment>
    <comment type="PTM">
        <text evidence="14">The reversible ADP-ribosylation of Arg-95 inactivates the nitrogenase reductase and regulates nitrogenase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14 16">Belongs to the NifH/BchL/ChlL family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.18.6.1" evidence="14"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96061.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591750.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSE0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00664"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.842"/>
    <dbReference type="OrthoDB" id="145464at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016163">
        <property type="term" value="F:nitrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02040">
        <property type="entry name" value="NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00533">
        <property type="entry name" value="NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030655">
        <property type="entry name" value="NifH/chlL_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000392">
        <property type="entry name" value="NifH/frxC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005977">
        <property type="entry name" value="Nitrogenase_Fe_NifH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01287">
        <property type="entry name" value="nifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009701">
        <property type="entry name" value="PRK13230.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42864">
        <property type="entry name" value="LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE IRON-SULFUR ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42864:SF2">
        <property type="entry name" value="LIGHT-INDEPENDENT PROTOCHLOROPHYLLIDE REDUCTASE IRON-SULFUR ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00142">
        <property type="entry name" value="Fer4_NifH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000363">
        <property type="entry name" value="Nitrogenase_iron"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00091">
        <property type="entry name" value="NITROGNASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00746">
        <property type="entry name" value="NIFH_FRXC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00692">
        <property type="entry name" value="NIFH_FRXC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51026">
        <property type="entry name" value="NIFH_FRXC_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 14 16" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7 14 15" id="KW-0013">ADP-ribosylation</keyword>
    <keyword evidence="8 14 16" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 14 16" id="KW-0408">Iron</keyword>
    <keyword evidence="11 14 16" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5 14 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12 14" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="6 14 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 14 16" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="8"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="modified residue" description="ADP-ribosylarginine; by dinitrogenase reductase ADP-ribosyltransferase" evidence="14 15">
        <location>
            <position position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR605977-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU003688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN96061.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="274" mass="29973" checksum="5E6EC7E083252C94" modified="2013-06-26" version="1">MKQIAFYGKGGIGKSTVVCNVAAALADNFKVMVVGCDPKHDCTISLRHGEEIPTVLDVLREKKDVDINDIVYKGYKGVYCVEAGGPKPGYGCAGRGVIVAIELLKKLDVFKRLGVDIVLYDILGDVVCGGFAMPLRLGLVKQIYIVTSSDYMAMYAANNICKGIKEFAKKGCRLAGLIYNVRGSLDAEDIVNEFAKRLGTKILEKIPNSLLISEAEIEGKTVIEYAPDSEIATIIKNLAEKIYKNNSGVIPEPLSNKELIEIGKSIKERIRRRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSV6</accession>
    <name>N6VSV6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Translation initiation factor 1A</fullName>
            <shortName evidence="5">aIF-1A</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">eif1a</name>
        <name evidence="10" type="ORF">J422_02954</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5 8">Seems to be required for maximal rate of protein biosynthesis. Enhances ribosome dissociation into subunits and stabilizes the binding of the initiator Met-tRNA(I) to 40 S ribosomal subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 7">Belongs to the eIF-1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96286.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590737.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSV6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00138"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.579"/>
    <dbReference type="OrthoDB" id="2586at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05793">
        <property type="entry name" value="S1_IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00216">
        <property type="entry name" value="aIF_1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006196">
        <property type="entry name" value="RNA-binding_domain_S1_IF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001253">
        <property type="entry name" value="TIF_eIF-1A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018104">
        <property type="entry name" value="TIF_eIF-1A_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00523">
        <property type="entry name" value="eIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003084">
        <property type="entry name" value="PRK04012.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003085">
        <property type="entry name" value="PRK04012.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21668">
        <property type="entry name" value="EIF-1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01176">
        <property type="entry name" value="eIF-1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00652">
        <property type="entry name" value="eIF1a"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01262">
        <property type="entry name" value="IF1A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50832">
        <property type="entry name" value="S1_IF1_TYPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 5 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S1-like" evidence="9">
        <location>
            <begin position="6"/>
            <end position="81"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007392"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00216"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96286.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="98" mass="11764" checksum="73BD2BBF3140AB0C" modified="2013-06-26" version="1">MEEQQIRVRIPNKEENEILAIVEQMLGASRMRVRCMDGKVRLARIPGRLKRKIWVKEGDIVIIKPWEVQGDEKCDIIWRYTKTQVEWLKRKGYLDELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSZ0</accession>
    <name>N6VSZ0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">RNA methylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_03114</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96316.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590774.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSZ0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00169"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.611"/>
    <dbReference type="OrthoDB" id="7080at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016423">
        <property type="term" value="F:tRNA (guanine) methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11715">
        <property type="entry name" value="THUMP_AdoMetMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000409">
        <property type="entry name" value="RNA methylase, PF01170 family"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000241">
        <property type="entry name" value="RlmKL-like_Mtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053943">
        <property type="entry name" value="RlmKL-like_Mtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054170">
        <property type="entry name" value="RlmL_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053485">
        <property type="entry name" value="tRNA_guanine-N2-MTase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040721">
        <property type="entry name" value="Trm14_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911">
        <property type="entry name" value="THUMP DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14911:SF13">
        <property type="entry name" value="TRNA (GUANINE(6)-N2)-METHYLTRANSFERASE THUMP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22020">
        <property type="entry name" value="RlmL_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01170">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01261">
        <property type="entry name" value="UPF0020"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="THUMP" evidence="8">
        <location>
            <begin position="43"/>
            <end position="157"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96316.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="381" mass="43624" checksum="23B6260283EDC3A5" modified="2013-06-26" version="1">MKFYSTLSPGLEDIAANELESLGAKILEKREGKGRIFLEGNLELIAKINYFSRTIERLNILLHREVLEDISLDEIYKRVYEINWEDWIKEEQSFAVRPLRVGEHNFTSIDIGRVAGEAVIKRYLSEKGVRLKVNLDEPDIIVRVELIFNELLVAIDTTGDEGLHKRGYRVYNHPAHLNATIAASLIYLSGWKDEEFLLDPMCGSGTIPIEGALIKRNIPIGKFRERRYGFSFVKIFGHELLDKIKNCYNENYRSYKIVGMDINSEYLEGAKKNAINANVIDTVKFLNGSAEELDKFFNSLDVIVTNPPYGIRMGKKRLIGKLYNNFLSSAKNLMHGDSRIVVITAESKLFKDAVIKNNFNIIKHFDVMFGGLLTKVYYIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT24</accession>
    <name>N6VT24_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Transcription elongation factor Spt5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nusG</name>
        <name evidence="4" type="synonym">spt5</name>
        <name evidence="8" type="ORF">J422_02659</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Stimulates transcription elongation.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer composed of Spt4 and Spt5. Interacts with RNA polymerase (RNAP).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SPT5 family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal Spt5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96346.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590571.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT24"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00084"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.521"/>
    <dbReference type="OrthoDB" id="371863at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032784">
        <property type="term" value="P:regulation of DNA-templated transcription elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006357">
        <property type="term" value="P:regulation of transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006368">
        <property type="term" value="P:transcription elongation by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06091">
        <property type="entry name" value="KOW_NusG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd09887">
        <property type="entry name" value="NGN_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.940">
        <property type="entry name" value="NusG, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00950">
        <property type="entry name" value="Spt5_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005100">
        <property type="entry name" value="NGN-domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006645">
        <property type="entry name" value="NGN-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036735">
        <property type="entry name" value="NGN_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005825">
        <property type="entry name" value="Ribosomal_uL24_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039659">
        <property type="entry name" value="SPT5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011590">
        <property type="entry name" value="Spt5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00405">
        <property type="entry name" value="KOW_elon_Spt5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125">
        <property type="entry name" value="SUPPRESSOR OF TY 5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11125:SF7">
        <property type="entry name" value="TRANSCRIPTION ELONGATION FACTOR SPT5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03439">
        <property type="entry name" value="Spt5-NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00738">
        <property type="entry name" value="NGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01108">
        <property type="entry name" value="RIBOSOMAL_L24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="NusG-like N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="7">
        <location>
            <begin position="88"/>
            <end position="115"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96346.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="146" mass="16228" checksum="040F63F33AC36206" modified="2013-06-26" version="1">MIFAVRTMVGQEKNIAELMYGRVEKENLDVYSILASEALKGYVLVEAESEGVVKELIKGMPRVKGLVKGTINIEEIEQLLTPKKIIENIEKGDVVEIIAGPFKGERAKVIKVDKNKEEITLELENATVPIPITLPVESVKIISKQK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT34</accession>
    <name>N6VT34_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Putative mRNA 3'-end processing factor 3</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_02709</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96356.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590592.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT34"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00094"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.531"/>
    <dbReference type="OrthoDB" id="40950at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd16295">
        <property type="entry name" value="TTHA0252-CPSF-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10890">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022712">
        <property type="entry name" value="Beta_Casp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050698">
        <property type="entry name" value="MBL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011108">
        <property type="entry name" value="RMMBL"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203:SF51">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11203">
        <property type="entry name" value="CLEAVAGE AND POLYADENYLATION SPECIFICITY FACTOR FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10996">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07521">
        <property type="entry name" value="RMMBL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01027">
        <property type="entry name" value="Beta-Casp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="7">
        <location>
            <begin position="14"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="domain" description="Beta-Casp" evidence="8">
        <location>
            <begin position="219"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96356.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="417" mass="46704" checksum="9EBF4EC10B2CFD4A" modified="2013-06-26" version="1">MVLLKFHGGCQEVGKSCVEVIGKEKRILLDCGASPNSGEIPNIENNLEVIVSHAHYDHCGAIPFYKFKKIYCTHPTADLMYITWKETLNISKAYKEEDIYRSLKSVETLNYYEEKDLGSLKFKLYNAGHILGSSSIYLEIDGKKILYTGDINEGPSRTVRSADTDIDEIDVLIIESTYGSPLDIKPSRKVLEKQLIEEVSETIEGGGKVIIPVFSVGRAQEILVVLNNYIRSGQLDAKIYTDGSLIHATGIYLSYKDWLNPKLKNTIESGINPFGDIKKADEKKVFSNEPCIIVSTSGMLQGGPVLKYLKLLKDEKNKLILTGHQAEGTLGKALEEGIREIKPFKNTIKIRGKVIKLEFSAHSDYNSLVRYIKKIPKPEKAIVMHGERYQSLSFAMTIWKTLKIPAYVPVEGTVIPL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTB0</accession>
    <name>N6VTB0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Geranylgeranyl reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_02360</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96431.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590344.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTB0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00353"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.464"/>
    <dbReference type="OrthoDB" id="46008at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016628">
        <property type="term" value="F:oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008654">
        <property type="term" value="P:phospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.9.10">
        <property type="entry name" value="D-Amino Acid Oxidase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011777">
        <property type="entry name" value="Geranylgeranyl_Rdtase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050407">
        <property type="entry name" value="Geranylgeranyl_reductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054715">
        <property type="entry name" value="GGR_cat"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02032">
        <property type="entry name" value="GG-red-SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685:SF18">
        <property type="entry name" value="DIGERANYLGERANYLGLYCEROPHOSPHOLIPID REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42685">
        <property type="entry name" value="GERANYLGERANYL DIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22578">
        <property type="entry name" value="GGR_cat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0274">FAD</keyword>
    <keyword evidence="2" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="1" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="5" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="7" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Digeranylgeranylglycerophospholipid reductase catalytic" evidence="8">
        <location>
            <begin position="158"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF22578"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96431.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="374" mass="41957" checksum="820EA11DA9520828" modified="2013-06-26" version="1">MEYDVVVIGGGIIGNITAKEIKGDVLIVEEHQSIGFPLQCAGIISKKCYESLEKPKGVVNKVRGAYIHTIKNTLKIESEKDMAYVMERKVMDKDIAIKAAKKCDILLKSYAEVEENKVIIKHGGEVIEIQPEVIVSAEGVRARTGMKLGLLKGREVLSACQFEMVNVKNIDDDFVHIFLDKRYSENFFVWIIPLEEDRVRVGLLDKGGGYNKLLKFINNKSEMFKNTSIVEFSVGALPIGYLKKTVKDNILLVGDSACHVKPLSGGGIYYGSLGGRIAGRVINEYLNGSGDLRRYDKEWRKEFGEELKTSLMIRKLFLKMGNETIDEIIKRIKDSDLVDYINKYGDMEKQSIIALKVLKSLGLSLGLKLIKEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTE6</accession>
    <name>N6VTE6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Methyl-coenzyme M reductase subunit gamma</fullName>
            <ecNumber evidence="9">2.8.4.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_02190</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Component of the methyl-coenzyme M reductase (MCR) I that catalyzes the reductive cleavage of methyl-coenzyme M (CoM-S-CH3 or 2-(methylthio)ethanesulfonate) using coenzyme B (CoB or 7-mercaptoheptanoylthreonine phosphate) as reductant which results in the production of methane and the mixed heterodisulfide of CoB and CoM (CoM-S-S-CoB). This is the final step in methanogenesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>coenzyme B + methyl-coenzyme M = methane + coenzyme M-coenzyme B heterodisulfide</text>
            <dbReference type="Rhea" id="RHEA:12532"/>
            <dbReference type="ChEBI" id="CHEBI:16183"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58411"/>
            <dbReference type="ChEBI" id="CHEBI:58596"/>
            <dbReference type="EC" id="2.8.4.1"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="8">
            <dbReference type="Rhea" id="RHEA:12533"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>coenzyme F430</name>
            <dbReference type="ChEBI" id="CHEBI:60540"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 9">One-carbon metabolism; methyl-coenzyme M reduction; methane from methyl-coenzyme M: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Hexamer of two alpha, two beta, and two gamma chains.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">MCR is a hexamer of two alpha, two beta, and two gamma chains, forming a dimer of heterotrimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the methyl-coenzyme M reductase gamma subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.4.1" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96461.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590264.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTE6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01186"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.430"/>
    <dbReference type="OrthoDB" id="52520at2157"/>
    <dbReference type="UniPathway" id="UPA00646">
        <property type="reaction ID" value="UER00699"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050524">
        <property type="term" value="F:coenzyme-B sulfoethylthiotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00539">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.320.20">
        <property type="entry name" value="Methyl-coenzyme M reductase, gamma subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009024">
        <property type="entry name" value="Me_CoM_Rdtase_Fd-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003178">
        <property type="entry name" value="Me_CoM_Rdtase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036994">
        <property type="entry name" value="Me_CoM_Rdtase_gsu_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03259">
        <property type="entry name" value="met_CoM_red_gam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02240">
        <property type="entry name" value="MCR_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000264">
        <property type="entry name" value="Meth_CoM_rd_gama"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55088">
        <property type="entry name" value="Methyl-coenzyme M reductase subunits"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001952"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR000264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96461.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="260" mass="30204" checksum="10F63386A19E7151" modified="2013-06-26" version="1">MAYKPQFYPGSTKIAQNRRNHMNPEVQLEKLRDIPDDDVVKIMGHRQPGEDYKTVHPPLEEMDLPEDYVRDLVEPINGAKEGHRIRYIQFTDSMYNAPAQPYDRARTYMWRFRGVDTGTLSGRQVIEMRESDLEAIAKNFLIDTAFFDPARCGIRGATVHGHSLRLDENGLMFDALQRYIYDEKTGHVLYVKDQVGRPLDEPVDVGEPLPEEKLREITTIYRKDNIGLRQDEELLTVIKRIHRARTFGGFLPVEDVFEKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTS4</accession>
    <name>N6VTS4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Small ribosomal subunit protein uS5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rps5p</name>
        <name evidence="8" type="synonym">rps5</name>
        <name evidence="12" type="ORF">J422_01515</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">With S4 and S12 plays an important role in translational accuracy.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6 8">Part of the 30S ribosomal subunit. Contacts protein S4.</text>
    </comment>
    <comment type="domain">
        <text evidence="8">The N-terminal domain interacts with the head of the 30S subunit; the C-terminal domain interacts with the body and contacts protein S4. The interaction surface between S4 and S5 is involved in control of translational fidelity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 10">Belongs to the universal ribosomal protein uS5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589964.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTS4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00431"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.298"/>
    <dbReference type="OrthoDB" id="38155at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.160.20:FF:000002">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.230.10:FF:000004">
        <property type="entry name" value="40S ribosomal protein S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01307_A">
        <property type="entry name" value="Ribosomal_uS5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000851">
        <property type="entry name" value="Ribosomal_uS5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047866">
        <property type="entry name" value="Ribosomal_uS5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005324">
        <property type="entry name" value="Ribosomal_uS5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005711">
        <property type="entry name" value="Ribosomal_uS5_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013810">
        <property type="entry name" value="Ribosomal_uS5_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018192">
        <property type="entry name" value="Ribosomal_uS5_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003125">
        <property type="entry name" value="PRK04044.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01020">
        <property type="entry name" value="uS5_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718:SF4">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13718">
        <property type="entry name" value="RIBOSOMAL S SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00333">
        <property type="entry name" value="Ribosomal_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03719">
        <property type="entry name" value="Ribosomal_S5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54768">
        <property type="entry name" value="dsRNA-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00585">
        <property type="entry name" value="RIBOSOMAL_S5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50881">
        <property type="entry name" value="S5_DSRBD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 8 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="S5 DRBM" evidence="11">
        <location>
            <begin position="49"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025844"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035255"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="217" mass="23917" checksum="8B919DEC256813EC" modified="2013-06-26" version="1">MRFNIDEWEPKTTVGRMVKEGEITDIDYILDNNLPILEPEIVDVLLPDLEEQVLDVKLVQRMHKSGRRARFRATVVVGNKNGYVGVGKGKAKEVGPAIRKAIAQAKKNIIRVRRGCGSWECGCGTAHSVPYRGYGKCGSTAIILLPAPKGVGLVAGDVAKAVLRLAGIKDVWTKTFGETRTTYNFAMATFEALKSLNFTRTMDKHKKVLGITEGRVF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU58</accession>
    <name>N6VU58_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DNA replication complex GINS family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00806</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589831.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU58"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00874"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.159"/>
    <dbReference type="OrthoDB" id="62268at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd11714">
        <property type="entry name" value="GINS_A_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="26"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="145" mass="17923" checksum="C84A79C81CC9D8C8" modified="2013-06-26" version="1">MYKELKNYFFNEIRSDTLLKLPEDFYEKVREYIKKLEDEKEKERAKYYYRELRKLRIYKAMYFDRENLLKEEEEILNKIEFIEEEPKGIYTIKDIEVVKVNKQFPQFTDGNFIYNLNKEEVITLDKKIATILEKHGIISKLRVRM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6VUB0</accession>
    <name>N6VUB0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Heavy-metal chelation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00426</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96781.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VUB0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00695"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.82"/>
    <dbReference type="OrthoDB" id="40399at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007161">
        <property type="entry name" value="DUF364"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025251">
        <property type="entry name" value="DUF4213"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04016">
        <property type="entry name" value="DUF364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13938">
        <property type="entry name" value="DUF4213"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159713">
        <property type="entry name" value="Dhaf3308-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF4213" evidence="2">
        <location>
            <begin position="22"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Putative heavy-metal chelation" evidence="1">
        <location>
            <begin position="120"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13938"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96781.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="29463" checksum="C9EA2CD4ED803969" modified="2013-06-26" version="1">MEKKDLISKVDIMLVKELKKKVLKRIDEDFEIVDFSFALPYTYVLIKGKKGNSIGLAMTLIEDIQFCESEFDEIDVRTFIEKVDSVNIIERTIGLATINAVSQYYLNLEGAKFDDATGIIKDVKKVAIIGNMKPIVNKLKNRGYEVYVFERNPRLKSIDTLSDCFEYNLLPNMDAVLISGTSLINDSIDMILERSKKAKIKVLVGATAQALPEFFKGYLTHIASVKIINIKKVLLNLKLGSSSKLFSNEYSRKYYYNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VUD6</accession>
    <name>N6VUD6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATP binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00140</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96811.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589717.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VUD6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01140"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.28"/>
    <dbReference type="OrthoDB" id="372052at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017178">
        <property type="term" value="F:diphthine-ammonia ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017183">
        <property type="term" value="P:protein histidyl modification to diphthamide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01994">
        <property type="entry name" value="AANH_PF0828-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1490.10:FF:000006">
        <property type="entry name" value="ATP binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000145">
        <property type="entry name" value="ATP-binding domain containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1490.10">
        <property type="entry name" value="putative n-type atp pyrophosphatase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002761">
        <property type="entry name" value="Diphthami_syn_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030662">
        <property type="entry name" value="DPH6/MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005237">
        <property type="entry name" value="MJ0570"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022427">
        <property type="entry name" value="MJ0570_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03679">
        <property type="entry name" value="arCOG00187"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00290">
        <property type="entry name" value="MJ0570_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00289">
        <property type="entry name" value="TIGR00289 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196:SF2">
        <property type="entry name" value="DIPHTHINE--AMMONIA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12196">
        <property type="entry name" value="DOMAIN OF UNKNOWN FUNCTION 71 DUF71 -CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01902">
        <property type="entry name" value="Diphthami_syn_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF039123">
        <property type="entry name" value="Diphthamide_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Diphthamide synthase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96811.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="223" mass="25406" checksum="64819E9E669175F7" modified="2013-06-26" version="1">MDVAVLYSGGKDSNYALYWAIKMGHNVKYLVNVESENKESYMFHIPNVHLTNLSSKASGIPLIKIYTKGEKEKEVEDLKEGLKELDIDAVVTGAVASVYQKSRIDKICEELKIKSLAPLWGKDPEWILRETSKLFEVIIVGVYAYGLGKEWLGKVINEDNIDKLIDICNKYGIHKAFEGGEAETFVLDSPVFKKRIVIEEAEIEWHETWGVYHIKKAKLVKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VWW3</accession>
    <name>N6VWW3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Haloacid dehalogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_07062</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000070">
        <property type="protein sequence ID" value="ENN95582.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594798.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VWW3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00851"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1367"/>
    <dbReference type="OrthoDB" id="63793at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95582.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="150" mass="16624" checksum="8D4E1B83837253BF" modified="2013-06-26" version="1">MSIILLDLNGTIAVDGKIKEGVKDRLNKLKNKAKIFILSADTYGTLNNILNDLNVEGIRINKENHEKESLAKLKILKELKKKFPNEKIIAIGNGSNDELMLKYSDLGICVINEEGASVKAILSADVVVKDINDALDLILKENRLKATLRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX88</accession>
    <name>N6VX88_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transcription factor CBF/NF-Y/histone domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_06254</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Binds and compact DNA (95 to 150 base pairs) to form nucleosome-like structures that contain positive DNA supercoils. Increases the resistance of DNA to thermal denaturation (in vitro).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Chromosome</location>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the archaeal histone HMF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95732.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593351.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX88"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00288"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1218"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046982">
        <property type="term" value="F:protein heterodimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22909">
        <property type="entry name" value="HFD_archaea_histone-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.20.10">
        <property type="entry name" value="Histone, subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050947">
        <property type="entry name" value="Archaeal_histone_HMF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003958">
        <property type="entry name" value="CBFA_NFYB_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009072">
        <property type="entry name" value="Histone-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050004">
        <property type="entry name" value="HmfB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF043032">
        <property type="entry name" value="archaea_histone"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47828">
        <property type="entry name" value="ARCHAEAL HISTONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47828:SF1">
        <property type="entry name" value="ARCHAEAL HISTONE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00808">
        <property type="entry name" value="CBFD_NFYB_HMF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47113">
        <property type="entry name" value="Histone-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0158">Chromosome</keyword>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription factor CBF/NF-Y/archaeal histone" evidence="8">
        <location>
            <begin position="3"/>
            <end position="65"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00008264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95732.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="70" mass="7760" checksum="A448F63AC33DDD4C" modified="2013-06-26" version="1">MGELPIAPCVRILKKAGATRVSEEAQKYFAEAIEELALEIAKKAVEIAKEAGRKTVKEEDVKEAVRQYTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXI8</accession>
    <name>N6VXI8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Lipoprotein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05623</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95847.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592691.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXI8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00269"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1097"/>
    <dbReference type="OrthoDB" id="65967at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95847.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="188" mass="22479" checksum="CD44F2EB31F547D0" modified="2013-06-26" version="1">MKKLLIILLIVIITGCINSEKSNYSKVDKNYKNNISDNINKFNKSFFNKSPINTSKVLSKENSKINYNFEKNIDIHKMFLPLNYDKELDFEYEIIKKYGNITFVISKEPLDISRAHIYNDVNIHPQKDKFGNYIIYEFIPKYGNLTKSYCYYRKIGDYYIILQSSNLSNLTEKLWKNWSYYLFSLFKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXL1</accession>
    <name>N6VXL1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Probable cobalt-precorrin-6B C(15)-methyltransferase (decarboxylating)</fullName>
            <ecNumber evidence="5">2.1.1.196</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cbiT</name>
        <name evidence="7" type="ORF">J422_05489</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the methylation of C-15 in cobalt-precorrin-6B followed by the decarboxylation of C-12 to form cobalt-precorrin-7.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Co-precorrin-6B + S-adenosyl-L-methionine = Co-precorrin-7 + S-adenosyl-L-homocysteine + CO2</text>
            <dbReference type="Rhea" id="RHEA:36067"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:70791"/>
            <dbReference type="ChEBI" id="CHEBI:72780"/>
            <dbReference type="EC" id="2.1.1.196"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 8/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the methyltransferase superfamily. Archaeal-type CbiT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.196" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000041">
        <property type="protein sequence ID" value="ENN95872.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592559.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXL1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01406"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1072"/>
    <dbReference type="OrthoDB" id="6027at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00229"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043776">
        <property type="term" value="F:cobalt-precorrin-6B C5-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00786">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023475">
        <property type="entry name" value="CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014008">
        <property type="entry name" value="Cbl_synth_MTase_CbiT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050714">
        <property type="entry name" value="Cobalamin_biosynth_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025714">
        <property type="entry name" value="Methyltranfer_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02469">
        <property type="entry name" value="CbiT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182">
        <property type="entry name" value="COBALT-PRECORRIN-6B C(15)-METHYLTRANSFERASE (DECARBOXYLATING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43182:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-7 C(5)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13847">
        <property type="entry name" value="Methyltransf_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase" evidence="6">
        <location>
            <begin position="27"/>
            <end position="106"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="36"/>
            <end position="40"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="57"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95872.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="177" mass="19988" checksum="FBAE4E2E4A24AA0A" modified="2013-06-26" version="1">MEFITEKDIPITKEEIRAISLYKLDLKESDTVVDIGCGSGAMTVDIAKRVKKVYAIDFNEKAIELTKKNLNKFNIRNCEVILAKAEDVLDKLEYNKAFIGGTKNLEKIIKILSNKEVKLIVANTILLESTYKAINLFEQMGYSVEAVNAFISYSKKISSGHMFLARNPITIIKAYKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXR4</accession>
    <name>N6VXR4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05179</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000033">
        <property type="protein sequence ID" value="ENN95932.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592245.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXR4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01221"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1013"/>
    <dbReference type="OrthoDB" id="65665at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95932.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="182" mass="21672" checksum="151B3E63D1A59082" modified="2013-06-26" version="1">MMKVYKISGNKAIILFIIIILFLLILFLLALPIIIFLFILILGYIFFKYKFKGFLKHIYYRLRRKKIKIIDNSNNGEIKINFRKSLENVDDDTKMFINYLKNIGAKVDKRYVYINGYKAYPIYKSSYPVNEIITLNYPEDVEAVILGLKGSPDNPKFLFLIPKEFLKRRMHIDEIKRFILNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXT6</accession>
    <name>N6VXT6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PUA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04900</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95952.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXT6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00511"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.960"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23947">
        <property type="entry name" value="PAPS_reductase-like_YbdN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050128">
        <property type="entry name" value="Sulfate_adenylyltrnsfr_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43196:SF2">
        <property type="entry name" value="PHOSPHOADENOSINE PHOSPHOSULFATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43196">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="136"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95952.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="483" mass="57016" checksum="DF55D4157AC72C1C" modified="2013-06-26" version="1">MSVIHYILSENMKTYIGKIHLKWCYNCNLPILGKKCDICNTEALPVKLTPPGDARLGFRYDIDFINECLEREFGAKSIEDGIILLNKLPGNLEAYEVIVDGIVKYILYYNEEKETWKIKLKLHGAYDLIEKGANKRIVKIKNDLLNILKDKSTLLRPGIIEITNDIERGDDVIIVNEDERVVGVGLAMLSSNEIEKIERGKIIKVRFFNKNFQNRKLERYKLEEALNLMIEANNSVIYNYEKNAIGFIKNSYEKIKKPVIVAYSGGKDSLVTLILTLKALKKENVEVIFIDTGLEFPETLKNIEEVENFFNIEIKRLEAENFWEKIKEYGIPSRDYRWCSEVCKLNPLKEYLKDREVLSFVGIRKYESFSRAKKRLIYRNTYIKNQINALPIFHWTALHVWIYILKEGAPYNKLYEKGFDRVGCYICPAMELGEMDRVRKLYPELWEKWENVLKEYANKYKLPEDWIRKGLWRWKKFHPKDIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VY69</accession>
    <name>N6VY69_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Periplasmic binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04278</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96057.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VY69"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00667"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.837"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01147">
        <property type="entry name" value="HemV-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050902">
        <property type="entry name" value="ABC_Transporter_SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535:SF34">
        <property type="entry name" value="MOLYBDATE-BINDING PROTEIN MOLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="50"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96057.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="284" mass="31700" checksum="021DA305C3F048D6" modified="2013-06-26" version="1">MNNKAILIISILLISSLALAGCMNENKVGEKRVIKDILGREVEIPKEVNRIVCIGPGCLRLIVYLNATDKVVGVESSEKLWTPYGRPYRLAHPELANLPIIGQGGPSPKPNIEKIIEVHPNVIFYTFATKDAADELQKKTGIPVVVLDYGVLGTFKNDKLYQSIRIAGKVLNKEDRAEEVINFIDKTLEDLNKRTMDINNKPSVYVGGVGYKGMRGIESTFSNFPPLMAINTKSVVDNEVSGYKWVSISKEKLLEWNPDYIFIDEGGFLLIKKIIKKIQISINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VY94</accession>
    <name>N6VY94_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 15">Fructose-1,6-bisphosphate aldolase/phosphatase</fullName>
            <shortName evidence="15">FBP A/P</shortName>
            <shortName evidence="15">FBP aldolase/phosphatase</shortName>
            <ecNumber evidence="6 15">3.1.3.11</ecNumber>
            <ecNumber evidence="15">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="primary">fbp</name>
        <name evidence="16" type="ORF">J422_04388</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="15">Catalyzes two subsequent steps in gluconeogenesis: the aldol condensation of dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (GA3P) to fructose-1,6-bisphosphate (FBP), and the dephosphorylation of FBP to fructose-6-phosphate (F6P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 15">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>beta-D-fructose 1,6-bisphosphate = D-glyceraldehyde 3-phosphate + dihydroxyacetone phosphate</text>
            <dbReference type="Rhea" id="RHEA:14729"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.1.2.13"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 15">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 15">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 15">Homooctamer; dimer of tetramers.</text>
    </comment>
    <comment type="domain">
        <text evidence="15">Consists of a single catalytic domain, but remodels its active-site architecture via a large structural change to exhibit dual activities.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 15">Belongs to the FBP aldolase/phosphatase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="6 15"/>
    <dbReference type="EC" id="4.1.2.13" evidence="15"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96077.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591783.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VY94"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00646"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.860"/>
    <dbReference type="OrthoDB" id="5829at2157"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02067">
        <property type="entry name" value="FBP_aldolase_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002803">
        <property type="entry name" value="FBPase_V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036076">
        <property type="entry name" value="FBPase_V_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041126">
        <property type="entry name" value="FBP_aldo_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE/PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38341:SF1">
        <property type="entry name" value="FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE_PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01950">
        <property type="entry name" value="FBPase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015647">
        <property type="entry name" value="FBPtase_archl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111249">
        <property type="entry name" value="Sulfolobus fructose-1,6-bisphosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="14 15" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="8 15" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="10 15" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="12 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="11 15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13 15" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton acceptor; for FBP phosphatase activity" evidence="15">
        <location>
            <position position="14"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor/acceptor; for FBP aldolase activity" evidence="15">
        <location>
            <position position="230"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with DHAP; for FBP aldolase activity" evidence="15">
        <location>
            <position position="233"/>
        </location>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="55"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="96"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <begin position="105"/>
            <end position="106"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="133"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="233"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="234"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <begin position="243"/>
            <end position="244"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>dihydroxyacetone phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="15">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>beta-D-fructose 1,6-bisphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00018635"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN96077.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="382" mass="42429" checksum="03E27A5B1C6B8A33" modified="2013-06-26" version="1">MESEKITISVIKADVGGLCGHTIAPDELLEACESVLEKAVDEIILDYYVTRCGDDIDLIMSHKLGCDNEKVHGLAWNAFEEATKVAKELKLYGAGQDLLADSFSGNVRGLGPGVAEMEFVERKSEPIVVFCCDKTDPTAFNYPLFKMFADPFNTAGLVFDPSMISGFRFEVHDVIEHKKVLLDCPEEMYMLLALIGDYEKYAIKRIYRRKDGEIAAVVSTEKINFIAGEYVGKDDPVAIVRAQSGFPAVGEVLEPFANPHFVPGWMRGSHWGPLMPVGEDDAIPTRFDGPPRIVALGFQVCNGMLIGPNDLFADKGFDKAREKALEMADIIRRMGPFQPHRLPATMMEYTTVPKVLKALEDRFIPLEGLELIEEGGKEEKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYA2</accession>
    <name>N6VYA2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF2085 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04438</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96087.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591806.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYA2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00636"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.870"/>
    <dbReference type="OrthoDB" id="65798at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019206">
        <property type="entry name" value="DUF2085_TM"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09858">
        <property type="entry name" value="DUF2085"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="121"/>
            <end position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96087.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="17217" checksum="24C309D493BFF927" modified="2013-06-26" version="1">MRYYKILFLFSIFYIGIFLAPYTAYLGESSDIFKYISFLIYAIYSTICHQLPSRSYFIFGHKMAVCARCFGIYTGVLIGMIVYPFIKINKLPKYYLIIALTPMAIDGITQIIGLRESFNELRFITGFIAGFTIVFYILPIVLTKFHKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYB7</accession>
    <name>N6VYB7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Molybdenum cofactor biosynthesis protein A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">moaA</name>
        <name evidence="9" type="ORF">J422_04558</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96107.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYB7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00614"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.894"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061798">
        <property type="term" value="F:GTP 3',8'-cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013485">
        <property type="entry name" value="MoaA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010505">
        <property type="entry name" value="MoaA_twitch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02668">
        <property type="entry name" value="moaA_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001199">
        <property type="entry name" value="PRK00164.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960:SF0">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06463">
        <property type="entry name" value="Mob_synth_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="1"/>
            <end position="189"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96107.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="257" mass="29738" checksum="0F4E9E5066DD11CE" modified="2013-06-26" version="1">MTPEEIGKIVDVAKKFGIRKVKISGGEPLIRRDITDIVREVSSRKLKDVSLTTNGILLEKLADKLKNSGLDRVNVSLDALNPELYKKITGGDVNKVINGIKRAIDVGLTPVKINFLAMKINICELEKIMDFCRELGAILQIIEFIPIKKELKNYYYDISKIEKELEEKADKIIVRKFQNRKKYFIDTLEVEVVKPMDNSKFCMNCSRIRLTYDGYLKPCLLRDDNLVDILTPLRRGENIEKCFLECINRREPYFKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYE2</accession>
    <name>N6VYE2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7 8">6,7-dimethyl-8-ribityllumazine synthase</fullName>
            <shortName evidence="7">DMRL synthase</shortName>
            <shortName evidence="7">LS</shortName>
            <shortName evidence="7">Lumazine synthase</shortName>
            <ecNumber evidence="3 7 8">2.5.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">ribH</name>
        <name evidence="9" type="ORF">J422_04038</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of 6,7-dimethyl-8-ribityllumazine by condensation of 5-amino-6-(D-ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>(2S)-2-hydroxy-3-oxobutyl phosphate + 5-amino-6-(D-ribitylamino)uracil = 6,7-dimethyl-8-(1-D-ribityl)lumazine + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:26152"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
            <dbReference type="EC" id="2.5.1.78"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Forms an icosahedral capsid composed of 60 subunits, arranged as a dodecamer of pentamers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the DMRL synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.78" evidence="3 7 8"/>
    <dbReference type="EMBL" id="APMM01000025">
        <property type="protein sequence ID" value="ENN96137.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591463.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYE2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00737"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.791"/>
    <dbReference type="OrthoDB" id="7610at2157"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00404"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000906">
        <property type="term" value="F:6,7-dimethyl-8-ribityllumazine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09211">
        <property type="entry name" value="Lumazine_synthase_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.960:FF:000003">
        <property type="entry name" value="6,7-dimethyl-8-ribityllumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="entry name" value="Lumazine/riboflavin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00178">
        <property type="entry name" value="Lumazine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034964">
        <property type="entry name" value="LS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00114">
        <property type="entry name" value="lumazine-synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058:SF0">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21058">
        <property type="entry name" value="6,7-DIMETHYL-8-RIBITYLLUMAZINE SYNTHASE DMRL SYNTHASE LUMAZINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="Lumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="75"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="43"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="67"/>
            <end position="69"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="72"/>
            <end position="73"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>5-amino-6-(D-ribitylamino)uracil</name>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="115"/>
        </location>
        <ligand>
            <name>(2S)-2-hydroxy-3-oxobutyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58830"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012664"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00178"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR00114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96137.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="138" mass="15411" checksum="F3D522C92B8B8367" modified="2013-06-26" version="1">MVKLALVVAEFNRDITYMMEKLAEEHAEFLGAEIKYKLYVPGVFDMPLAIKKLLEKDDVDAVVAIGCVIEGETEHDEIVVHNAARKIADLSVQYDKPVTLGISGPGMTRLQAHERVDYAKRAVEAAVKMVKRLKALEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYG4</accession>
    <name>N6VYG4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit Rpo11</fullName>
            <ecNumber evidence="5">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit L</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpo11</name>
        <name evidence="5" type="synonym">rpoL</name>
        <name evidence="7" type="ORF">J422_03868</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the archaeal Rpo11/eukaryotic RPB11/RPC19 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96162.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591372.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYG4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00404"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.758"/>
    <dbReference type="OrthoDB" id="24205at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06927">
        <property type="entry name" value="RNAP_L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.10">
        <property type="entry name" value="RNA polymerase, RBP11-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00261">
        <property type="entry name" value="RNApol_arch_Rpo11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036603">
        <property type="entry name" value="RBP11-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009025">
        <property type="entry name" value="RBP11-like_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008193">
        <property type="entry name" value="RNA_pol_Rpb11_13-16kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022905">
        <property type="entry name" value="Rpo11-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002234">
        <property type="entry name" value="PRK01146.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I,II,III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13946:SF28">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I AND III SUBUNIT RPAC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13656">
        <property type="entry name" value="RNA_pol_L_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55257">
        <property type="entry name" value="RBP11-like subunits of RNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01154">
        <property type="entry name" value="RNA_POL_L_13KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed RNA polymerase RBP11-like dimerisation" evidence="6">
        <location>
            <begin position="13"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF13656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96162.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="96" mass="10896" checksum="A2996F42364451C3" modified="2013-06-26" version="1">MEIKILERKDNLIEIELIGEDHSLPNLLKDILLTKEGVKMASYSIEHPLLDPETGRYISNPVITVITEGGRDPLEVLKEALKDIIKMCDTLLEELK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYQ6</accession>
    <name>N6VYQ6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease J</fullName>
            <shortName evidence="8">RNase J</shortName>
            <ecNumber evidence="8">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnj</name>
        <name evidence="10" type="ORF">J422_03338</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">An RNase that has 5'-3' exonuclease activity. May be involved in RNA degradation.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 2 Zn(2+) ions per subunit. It is not clear if Zn(2+) or Mg(2+) is physiologically important.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the metallo-beta-lactamase superfamily. RNA-metabolizing metallo-beta-lactamase-like family. Archaeal RNase J subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="8"/>
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        <property type="protein sequence ID" value="ENN96262.1"/>
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        <property type="entry name" value="RIBONUCLEASE J"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
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        <property type="match status" value="1"/>
    </dbReference>
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        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
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        <property type="entry name" value="UPF0036"/>
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    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 8" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="4 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="9">
        <location>
            <begin position="15"/>
            <end position="224"/>
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            <position position="81"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
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            <name>Zn(2+)</name>
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        <ligand>
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            <name>Zn(2+)</name>
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        <location>
            <position position="151"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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            <position position="173"/>
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        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
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            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <label>1</label>
            <note>catalytic</note>
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            <name>substrate</name>
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            <name>Zn(2+)</name>
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        <source>
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        <source>
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    </evidence>
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        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
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    </evidence>
    <sequence length="448" mass="49974" checksum="482EC1128788EEF4" modified="2013-06-26" version="1">MKLEIVAIGGYEEVGRNMTAINVDGEIIIIDMGIRLDRVLIHEDTDISKLHSLELIEKGIIPNDTVIKNLDGEVKAIVLSHGHLDHIGAVPKLAHRYNAPIIGTPYTIELVKREILSEKKFDVRNPLVVLNAGEAIDLTPNITLEFVRITHSIPDSVLPVLHTPYGTIVYGNDFKLDNFPVVGERPDYRAIKRLGKNGVLCFISETTRVNHEGKTPPEIIASGLLKNDLLAADNDKNGVIVTTFSSHIARIKSITEIAEKMGRTPVLLGRSMLRYCGIAQEIGIVKFPEDLRIYGDPSSIEMALKMIMKEGKENYLIIATGHQGEEGAVLSRMATNKTSYKFDKYDCVIFSADPIPNPINAAQRYLLESRLKLLGVRIFKGAHVSGHASKEDHRDMLRWLNPEHLIPSHGDFNLTAEYTKLAEEEGYRLGEDVHLLRNGQCLSFERVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZB2</accession>
    <name>N6VZB2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 19">Tetrahydromethanopterin S-methyltransferase subunit C</fullName>
            <ecNumber evidence="18 19">7.2.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 19">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">mtrC</name>
        <name evidence="20" type="ORF">J422_02170</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 19">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="7.2.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 19">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 19">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Cell membrane</location>
            <topology evidence="2 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the MtrC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.1.4" evidence="18 19"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96457.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590240.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZB2"/>
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    <dbReference type="PATRIC" id="fig|1069083.5.peg.426"/>
    <dbReference type="OrthoDB" id="60591at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01096">
        <property type="entry name" value="MtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005865">
        <property type="entry name" value="THM_MeTrfase_su_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01148">
        <property type="entry name" value="mtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04211">
        <property type="entry name" value="MtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006530">
        <property type="entry name" value="MtrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 19" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="9 19 20" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 19" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 19" id="KW-1278">Translocase</keyword>
    <keyword evidence="11 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="18"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="73"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="100"/>
            <end position="120"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="141"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="173"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="206"/>
            <end position="238"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002533"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00004839"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00007607"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00011616"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00015131"/>
        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
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        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
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        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029817"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ENN96457.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="264" mass="27467" checksum="9567CAC66DFA5F3E" modified="2013-06-26" version="1">MAHGGGGHAAELYPEEQIFGIGIALALIGCYLAHFLSGYGLSMLIGGLLVSASCVAGANTVRKVAAYGLGTGVPSIGMLSLGMGTVSAIAGVLITKSLNIPYLAGPILALVISAIVGYIVGKLTVKPVGMKIPIMVRSMTFLSIAGAMALLGYSVAYAGSLDPNVFINALNDGIMAIAFIASGMAILHPFNACLGPNESHKRTLTLAIACGLLTWFIFSVIKLDIVSVVVSIILWAIVYVKFVKMSFNDACCVLYIPEIPKKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZL0</accession>
    <name>N6VZL0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Signal recognition particle 19 kDa protein</fullName>
            <shortName evidence="5">SRP19</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">srp19</name>
        <name evidence="6" type="ORF">J422_01995</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in targeting and insertion of nascent membrane proteins into the cytoplasmic membrane. Binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the signal recognition particle protein translocation system, which is composed of SRP and FtsY. Archaeal SRP consists of a 7S RNA molecule of 300 nucleotides and two protein subunits: SRP54 and SRP19.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SRP19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96542.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590166.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZL0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00244"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.391"/>
    <dbReference type="OrthoDB" id="56356at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048500">
        <property type="term" value="C:signal recognition particle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008312">
        <property type="term" value="F:7S RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006617">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.30">
        <property type="entry name" value="Signal recognition particle, SRP19-like subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00305">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002778">
        <property type="entry name" value="Signal_recog_particle_SRP19"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036521">
        <property type="entry name" value="SRP19-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022938">
        <property type="entry name" value="SRP19_arc-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KD PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR17453:SF0">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE 19 KDA PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01922">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69695">
        <property type="entry name" value="SRP19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 5" id="KW-0733">Signal recognition particle</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96542.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="86" mass="10235" checksum="89AC105E829BA9F1" modified="2013-06-26" version="1">MIIWPAYIDKNKTRKEGRRVPKNLAIENPNLKDIEKALKKLGLEGKIIRDKRYPRQHWEVCGCIELDYKGNKLKLLKEICKIIKNY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZM6</accession>
    <name>N6VZM6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02045</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96552.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590191.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZM6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00254"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.401"/>
    <dbReference type="OrthoDB" id="65224at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058495">
        <property type="entry name" value="DUF8182"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021778">
        <property type="entry name" value="Se/S_carrier-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26554">
        <property type="entry name" value="DUF8182"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11823">
        <property type="entry name" value="Se_S_carrier"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative Se/S carrier protein-like" evidence="1">
        <location>
            <begin position="24"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="domain" description="DUF8182" evidence="2">
        <location>
            <begin position="100"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF26554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96552.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="188" mass="21421" checksum="BB718D2274561651" modified="2013-06-26" version="1">MILKKIKKLLRKKGNEEKEFEGRGLIIFKSVKDAMKAESILKNYNVRVVAPPYEIREGCDLALEYDLVDELGIKRELEKNNINPLKFIPLNDYSLKPLELIKIKEIDGFIMVRCGNMKITIDKEGNIVNISGGGCPDVPYLALKLKGKNIKDIKEDETPKSLGYTLCAYTLNKAFEKAKELINLKNKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZQ3</accession>
    <name>N6VZQ3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL30</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl30p</name>
        <name evidence="4" type="synonym">rpl30</name>
        <name evidence="6" type="ORF">J422_01520</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the universal ribosomal protein uL30 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96562.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589965.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZQ3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00432"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.299"/>
    <dbReference type="OrthoDB" id="6379at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000463">
        <property type="term" value="P:maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01657">
        <property type="entry name" value="Ribosomal_L7_archeal_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.15.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1390.20">
        <property type="entry name" value="Ribosomal protein L30, ferredoxin-like fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01371_A">
        <property type="entry name" value="Ribosomal_uL30_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036919">
        <property type="entry name" value="Ribo_uL30_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039699">
        <property type="entry name" value="Ribosomal_uL30"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005997">
        <property type="entry name" value="Ribosomal_uL30_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018038">
        <property type="entry name" value="Ribosomal_uL30_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035808">
        <property type="entry name" value="Ribosomal_uL30_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016082">
        <property type="entry name" value="Ribosomal_uL30_ferredoxin-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004711">
        <property type="entry name" value="PRK06049.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01309">
        <property type="entry name" value="uL30_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11524:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00327">
        <property type="entry name" value="Ribosomal_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55129">
        <property type="entry name" value="Ribosomal protein L30p/L7e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00634">
        <property type="entry name" value="RIBOSOMAL_L30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL30-like ferredoxin-like fold" evidence="5">
        <location>
            <begin position="3"/>
            <end position="53"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00327"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96562.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="154" mass="17912" checksum="286BE8F0C2E551E7" modified="2013-06-26" version="1">MAYAVIRVRGRIGVRGDIADTLKMLRLHKVNHCVIIPETDTYKGMLRKAKDYITWGEINKETLKKLILKRGRLPGNKRVNEEIIKELTGMDIDELCEKIINGEIKIKDLPIKPVFRLHPPKRGWERRGIKKHFKEGGALGYRGEKINELLERMM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZR4</accession>
    <name>N6VZR4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01555</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96567.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589970.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZR4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00438"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.306"/>
    <dbReference type="OrthoDB" id="65227at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96567.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="248" mass="28862" checksum="F1ACE5A7BBA92704" modified="2013-06-26" version="1">MIKIRALIEVKGNIKNIVDKEFERIKEDIKNYNPKLVFEETEEGEFYTKIGEFEISFNNLLEYLEFCLKYGADIEVVSPDKLVLDVDEINKALAFVISFFKKIYEKYNVVYKMDLKEKDINVEEYKLGRFDEDEILELSEEYIRVKCVFEGVGKDEETVIKKLISTLDSENIIINKVLTQNFDKDKFNGLVAIDMFCKPFELFEIAYKFLPIAISVQKNKVEINLSELQDIGNELSGAVFEIAHIIKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZV7</accession>
    <name>N6VZV7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CopG family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01423</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589946.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZV7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00004"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.279"/>
    <dbReference type="OrthoDB" id="25654at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd22231">
        <property type="entry name" value="RHH_NikR_HicB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1150">
        <property type="entry name" value="ACT-like. Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027271">
        <property type="entry name" value="Acetolactate_synth/TF_NikR_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002145">
        <property type="entry name" value="CopG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050192">
        <property type="entry name" value="CopG/NikR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010985">
        <property type="entry name" value="Ribbon_hlx_hlx"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34719:SF2">
        <property type="entry name" value="NICKEL-RESPONSIVE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01402">
        <property type="entry name" value="RHH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47598">
        <property type="entry name" value="Ribbon-helix-helix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribbon-helix-helix protein CopG" evidence="1">
        <location>
            <begin position="5"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="135" mass="15823" checksum="87D30AFB679160AB" modified="2013-06-26" version="1">MVNVERVSLSLPKFLLGEIDRLMKRKGYSSRSELIRDAIRKYILESNLPLEDKEVGGIIILVYSPNKENIEFLEKLFLEYKDIINSINQSYIKTSCGKNKKIETYIVEGNAKKILEFYENIIKIPEKIYDKIIIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6W001</accession>
    <name>N6W001_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01001</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000006">
        <property type="protein sequence ID" value="ENN96672.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589868.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W001"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01018"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.195"/>
    <dbReference type="OrthoDB" id="9837at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001270">
        <property type="entry name" value="ClpA/B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045427">
        <property type="entry name" value="MoxR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041538">
        <property type="entry name" value="RavA-like_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050513">
        <property type="entry name" value="RavA_ATPases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32204">
        <property type="entry name" value="ATPASE RAVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32204:SF0">
        <property type="entry name" value="ATPASE RAVA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17868">
        <property type="entry name" value="AAA_lid_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20030">
        <property type="entry name" value="bpMoxR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00300">
        <property type="entry name" value="CLPPROTEASEA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="1">
        <location>
            <begin position="29"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96672.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="371" mass="43393" checksum="6DA6CAC03F320703" modified="2013-06-26" version="1">MLQKIREELNSYFLERREEIDIALTSILAGEHTLFLGPPGTAKSQLIRAIAEHINANYFEKLITRFTTEDEIFGPLSIKELKENDRYVRKTEGYLPKAEVAFLDEIFKANSSILNSLLSIINERIYHNGDKIEKVPLISLFSASNELPEESELLAFYDRFLFRKIVRGIRGYNNLIRLLDLEDYKAKTKIEIDELKELQKEAMDVKIDNIKEDIIKIKLSLEGEGINISDRRLKKAVKAIKCYSYLNGKEKAEIEDLEILRFIFWNEPEEFYKTSIEIFKISNHFSGYAFEQREILNSLVNELKNIDKERIKLGGVEYRKCLEILGKLNSMVLTLKEVRAKAIDENKPHQYIDDTIEEIYSFKKYVEELLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6W0C2</accession>
    <name>N6W0C2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Transketolase central region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_00195</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>thiamine diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the transketolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96822.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589728.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W0C2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01129"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.39"/>
    <dbReference type="OrthoDB" id="6779at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07033">
        <property type="entry name" value="TPP_PYR_DXS_TK_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000129">
        <property type="entry name" value="Transketolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051157">
        <property type="entry name" value="PDH/Transketolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005475">
        <property type="entry name" value="Transketolase-like_Pyr-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020826">
        <property type="entry name" value="Transketolase_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033248">
        <property type="entry name" value="Transketolase_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43825">
        <property type="entry name" value="PYRUVATE DEHYDROGENASE E1 COMPONENT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43825:SF1">
        <property type="entry name" value="TRANSKETOLASE-LIKE PYRIMIDINE-BINDING DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02779">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02780">
        <property type="entry name" value="Transketolase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00861">
        <property type="entry name" value="Transket_pyr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00802">
        <property type="entry name" value="TRANSKETOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0786">Thiamine pyrophosphate</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Transketolase-like pyrimidine-binding" evidence="5">
        <location>
            <begin position="8"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023052"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96822.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="316" mass="34841" checksum="7ECB0A25F50EED0C" modified="2013-06-26" version="1">MKLTGVYKGMRKGYGETLIELGRKYENLVVLDADLSSSTQTAMFAKEFPDRFFNAGVAEQNLMGMAAGLATTGKIVFASSFAMFAAGRPWEIIRNLIAYPKLNVKIVATHAGITVGEDGASHQMCEDIAIMRAIPNMVVIAPTDYYHTKNVIREIAKYNGPVYVRMPRRDTEIIYESEEEASFKIGEGKILFDGDDITIIATGEEVPEALRAREILKEHGISAQIIEMATIKPLDEKILKKSNNFIVTVEDHNIIGGLGSAVAEAIVNDDKKLLRIGINDVFGKSGKAEELLKYYGLDGESVAKRILKVIKNEKGY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTC1</accession>
    <name>N6UTC1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_07217</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000081">
        <property type="protein sequence ID" value="ENN95554.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004595311.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000081.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTC1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00994"/>
    <dbReference type="OrthoDB" id="46222at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016758">
        <property type="term" value="F:hexosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22916:SF3">
        <property type="entry name" value="UDP-GLCNAC:BETAGAL BETA-1,3-N-ACETYLGLUCOSAMINYLTRANSFERASE-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="305"/>
            <end position="323"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="2">
        <location>
            <begin position="4"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95554.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="331" mass="39172" checksum="A9E40D2BB27FF65D" modified="2013-06-26" version="1" fragment="single">PLLSICIPTYNRARYLKDALDSIIKQINENNKDKVEICISDNASEDNTEELIKEYQKKSPIPIIYHRNKKNMGADYNYLKVVEIANGEYCWLLGSDDIIEDGGIDTVLSEIEKNKDVDIFTFDRYIYSKNLNNKHTKEVDEPKTRKLGKDYIFNNKIECLKYSSGNWGYISVIVVNRKKWLSVKGYEKWIGSAYVHVYIIYSLIKQGSKVKFINKHLVGWRSDNDSFLHELGEYRRFKIDINYVYIAEDVFGKDSEEYKIVKDMLINRSGFWGRITGAKFKGLSYKFYVNAFKDLFPIYKDHPKFWLWYVPLMIIPSFVYRFIRFIIKGKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTI8</accession>
    <name>N6UTI8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">TRK system potassium uptake protein TrkA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_06812</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a potassium transport system.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000062">
        <property type="protein sequence ID" value="ENN95634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594213.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTI8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01089"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1322"/>
    <dbReference type="OrthoDB" id="24929at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015079">
        <property type="term" value="F:potassium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1450">
        <property type="entry name" value="Regulator of K+ conductance, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006036">
        <property type="entry name" value="K_uptake_TrkA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006037">
        <property type="entry name" value="RCK_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036721">
        <property type="entry name" value="RCK_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050721">
        <property type="entry name" value="Trk_Ktr_HKT_K-transport"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833">
        <property type="entry name" value="POTASSIUM CHANNEL PROTEIN 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43833:SF5">
        <property type="entry name" value="TRK SYSTEM POTASSIUM UPTAKE PROTEIN TRKA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02080">
        <property type="entry name" value="TrkA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00335">
        <property type="entry name" value="KUPTAKETRKA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116726">
        <property type="entry name" value="TrkA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51202">
        <property type="entry name" value="RCK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="4" id="KW-0630">Potassium</keyword>
    <keyword evidence="3" id="KW-0633">Potassium transport</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="7">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="domain" description="RCK C-terminal" evidence="8">
        <location>
            <begin position="136"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003660"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022538"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="219" mass="24524" checksum="666809E2CA50D4C6" modified="2013-06-26" version="1">MYIIIAGIGRIGYTLGKSLSERYDVVLIDINKKICDKVAKEIDALVINGDCTKTKTLEDAGIDDADIYIAVTGKDEVNLISCLLAKKYYGVEKTISRISEIEYKDIFERLGVDIVISPELIAAHYIEKLIERPGVLDLTIIGRGEAEIIELIIPEDSEVANKKIKELKKPEDYLIIAVYEGNNLKIPSGETVLKPGDRVLVLVKKDKSEIIRRMFRGEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTJ2</accession>
    <name>N6UTJ2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">UDP-glucose 6-dehydrogenase</fullName>
            <ecNumber evidence="3 7">1.1.1.22</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_06763</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>UDP-alpha-D-glucose + 2 NAD(+) + H2O = UDP-alpha-D-glucuronate + 2 NADH + 3 H(+)</text>
            <dbReference type="Rhea" id="RHEA:23596"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58052"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="1.1.1.22"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.22" evidence="3 7"/>
    <dbReference type="EMBL" id="APMM01000059">
        <property type="protein sequence ID" value="ENN95639.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594060.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTJ2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00983"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1314"/>
    <dbReference type="OrthoDB" id="59839at2157"/>
    <dbReference type="UniPathway" id="UPA00038">
        <property type="reaction ID" value="UER00491"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003979">
        <property type="term" value="F:UDP-glucose 6-dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006065">
        <property type="term" value="P:UDP-glucuronate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.100">
        <property type="entry name" value="Cytochrome c1, transmembrane anchor, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017476">
        <property type="entry name" value="UDP-Glc/GDP-Man"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014027">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036220">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014026">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001732">
        <property type="entry name" value="UDP-Glc/GDP-Man_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028357">
        <property type="entry name" value="UDPglc_DH_bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03026">
        <property type="entry name" value="NDP-sugDHase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43750">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE TUAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43750:SF3">
        <property type="entry name" value="UDP-GLUCOSE 6-DEHYDROGENASE TUAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00984">
        <property type="entry name" value="UDPG_MGDP_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03720">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03721">
        <property type="entry name" value="UDPG_MGDP_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500134">
        <property type="entry name" value="UDPglc_DH_bac"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000124">
        <property type="entry name" value="UDPglc_GDPman_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00984">
        <property type="entry name" value="UDPG_MGDP_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52413">
        <property type="entry name" value="UDP-glucose/GDP-mannose dehydrogenase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 10" id="KW-0520">NAD</keyword>
    <keyword evidence="4 7" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UDP-glucose/GDP-mannose dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="313"/>
            <end position="425"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="260"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="146"/>
            <end position="149"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="149"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="249"/>
            <end position="253"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="257"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="263"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="327"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047473"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000124"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR500134-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95639.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="441" mass="50135" checksum="3426965F3A287EC2" modified="2013-06-26" version="1">MKISVVGTGYVGLIQAVGLAELGFEVVGIDIDEMKIKQLNKGKSPLYEEDLEELLKKHINKNLTFTTSYEPIKDSDVVFLCVGTPQDAEGNADLRFLFSAVEKMKDYLDEKYRVIVIKSTVPVGTNRKVKEILKGYNVDVVSNPEFLREGIAVYDFFNPERIVLGFEDLSNKKPIEIMETIYGYFKEKNIPFIITNWETSELIKYASNAFLATKISFINELAKLSDKVGADIKTISYAMGLDKRIGDKFLNAGIGYGGSCFPKDVKALIKQFENNNIEPILIKATDKVNEEQIKWFFEKIKGYYGNLNGKTFAVLGLAFKPNTDDLRESRAIKLIDMLLDSGAIVKGFDYVEKARENTINMYKLNKSKIVYGYNLYVLDDIYEAVKDVDGIIIATEYDYNKEDWKKIGKLVKEKVIFDGRNVLDVKKVKKLGFKYYGVGRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTR1</accession>
    <name>N6UTR1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12 13">Pyruvate kinase</fullName>
            <ecNumber evidence="3 12 13">2.7.1.40</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">J422_06229</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>pyruvate + ATP = phosphoenolpyruvate + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:18157"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.40"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the pyruvate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.40" evidence="3 12 13"/>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95729.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593345.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTR1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00292"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1213"/>
    <dbReference type="OrthoDB" id="56298at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00188"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030955">
        <property type="term" value="F:potassium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004743">
        <property type="term" value="F:pyruvate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.33.10">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001697">
        <property type="entry name" value="Pyr_Knase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011037">
        <property type="entry name" value="Pyrv_Knase-like_insert_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015793">
        <property type="entry name" value="Pyrv_Knase_brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015806">
        <property type="entry name" value="Pyrv_Knase_insert_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01064">
        <property type="entry name" value="pyruv_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11817">
        <property type="entry name" value="PYRUVATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00224">
        <property type="entry name" value="PK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01050">
        <property type="entry name" value="PYRUVTKNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50800">
        <property type="entry name" value="PK beta-barrel domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 13" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="7 13 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="9 13" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 16" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate kinase barrel" evidence="14">
        <location>
            <begin position="1"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="15">
        <location>
            <begin position="327"/>
            <end position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008663"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012142"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR01064"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN95729.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="434" mass="49079" checksum="942E1E95B2DE57E6" modified="2013-06-26" version="1">MRKTKILITLGPSIENCLDKAVNLIDGVRFNMSHANINYCMKFLKFLNKHNIAKLMDLKGIKIRIKRTKKKFLKENEIVEIGEDLEFNYDVNMKSGDRIIINDGKVVLEVIEDNKAVVIVGGEVRENMGANFPDSDIEAEIISDEDRENIRFACDEEFEYIALSFVRDKEDVKLLRDYINDFKGDCDIIAKIETKKALKNLENIAKNSDGLMVARGDLGVEIDIEYIPIEQKRIIETANRFGIISITATQILDSMVNNPYPTRAEVTDIANAIYDGTDCLMLSNETAIGKYPLKAIEVLNKVAEVADKHEEFIRKTDLDVFNVEEGLAYAVSSLYKKLPIKLTITPTYSGRTAKIISKLRKRIIAPTPNINTLRKLKLVYGVESCLMEEFNNIDNIISACREIAKKEIGSGLYLLVFGHPIGERKSNTIKVESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTT6</accession>
    <name>N6UTT6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL10</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Acidic ribosomal protein P0 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl10</name>
        <name evidence="6" type="synonym">rplP0</name>
        <name evidence="9" type="ORF">J422_06155</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593261.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTT6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00550"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1199"/>
    <dbReference type="OrthoDB" id="30930at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000027">
        <property type="term" value="P:ribosomal large subunit assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05795">
        <property type="entry name" value="Ribosomal_P0_L10e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.105.20:FF:000001">
        <property type="entry name" value="60S acidic ribosomal protein P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1730">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.760">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00280">
        <property type="entry name" value="Ribosomal_uL10_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050323">
        <property type="entry name" value="Ribosomal_protein_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001790">
        <property type="entry name" value="Ribosomal_uL10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040637">
        <property type="entry name" value="Ribosomal_uL10-like_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043164">
        <property type="entry name" value="Ribosomal_uL10-like_insert_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043141">
        <property type="entry name" value="Ribosomal_uL10-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022909">
        <property type="entry name" value="Ribosomal_uL10_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003096">
        <property type="entry name" value="PRK04019.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003098">
        <property type="entry name" value="PRK04019.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P0"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45699:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00466">
        <property type="entry name" value="Ribosomal_L10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17777">
        <property type="entry name" value="RL10P_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF160369">
        <property type="entry name" value="Ribosomal protein L10-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 9" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL10-like insertion" evidence="8">
        <location>
            <begin position="117"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="302"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="7">
        <location>
            <begin position="308"/>
            <end position="327"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF17777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="337" mass="36833" checksum="EA5A4CC1740FFA43" modified="2013-06-26" version="1">MTTKVKPHVAPWKIEEVKTLKSLIKSKPVVAIVDMMDVPAPQLQEIRAKIRDKVKLRMSRNTLIKRAIKEASEELNNPKLAELANYVERGAAILVTDMNPFKLYKLLEENKSPAPVKGGQIAPCDIKVEKGSTGMPPGPFLSELKAVGIPAAIEKGKIAIKEDKVVVRKGEVVSPQLAAVLNKLGIKPIKVGLDILAVYEDGIIYTPEVLKIDESKLLSDIQTAYQHAMNLAFNIAYPAKEVLPMLIQKAFMEAKALALESCYVTKDTAGDILAKAQAQALALAKLLPDEALDEEIKNLMSSAPAPAVEEKKEEAKEEKKEEKKEEETAAAGLALLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUB6</accession>
    <name>N6UUB6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_04835</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95939.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592050.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUB6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00523"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.947"/>
    <dbReference type="OrthoDB" id="2305at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031419">
        <property type="term" value="F:cobalamin binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.280">
        <property type="entry name" value="Cobalamin-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051198">
        <property type="entry name" value="BchE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006158">
        <property type="entry name" value="Cobalamin-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409">
        <property type="entry name" value="ANAEROBIC MAGNESIUM-PROTOPORPHYRIN IX MONOMETHYL ESTER CYCLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43409:SF17">
        <property type="entry name" value="METHYLTHIOTRANSFERASE MJ0865-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02310">
        <property type="entry name" value="B12-binding"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="190"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="7">
        <location>
            <begin position="429"/>
            <end position="492"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95939.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="492" mass="56031" checksum="FEADDE234AA91F37" modified="2013-06-26" version="1">MKALIIDCLAVNDGKRVLARDVIGAGPRTVKGILESEGLEAKIIPYEDFNIKKVRGFDIIFISAMTSDFKAVKKLVKELKLKENKKVIIGGPIASDIYLLNKIEADISIIGEGEITIRELIKKDFNPEGIKGTTYWDEELKINPFREILKDLSLITPSKDIKDYKNYFSARVYVEVVRGCSNFKRALLLCKNKKCSLCKEGSLYCPSNIKPGCGFCSVPSLFGYARSRDEEVILEELEELFKQGVKKAVLSAPDFLDYKRDGYNPYKPEPNYEAIESLIDNAKDLADKYDANVMVENIKANLFNEKIAKILSKLNTTIYIGCESGDKKHCELLGRPSYPEDVLKAVKIAKKYNLKAQVYFIYGLPGENKKTVENTIKFMHKIKPYIDKITVYKFRPLPMSAFQEFKPKITEYSLKIKKEAKKINYEIKKRYIGKILDVIIAEKHLKNDDYAVGYLKDSGLVVFVKNGNKYIGRVKKVKIIKAHEKYLEGVII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUK2</accession>
    <name>N6UUK2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Indole-3-glycerol phosphate synthase</fullName>
            <shortName evidence="11">IGPS</shortName>
            <ecNumber evidence="4 11">4.1.1.48</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">trpC</name>
        <name evidence="13" type="ORF">J422_04740</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 11">
            <text>1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate + H(+) = (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:23476"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58613"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="EC" id="4.1.1.48"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the TrpC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.48" evidence="4 11"/>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96019.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUK2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00967"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.927"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00043"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004425">
        <property type="term" value="F:indole-3-glycerol-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004640">
        <property type="term" value="F:phosphoribosylanthranilate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00331">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00134_A">
        <property type="entry name" value="IGPS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045186">
        <property type="entry name" value="Indole-3-glycerol_P_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013798">
        <property type="entry name" value="Indole-3-glycerol_P_synth_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001468">
        <property type="entry name" value="Indole-3-GlycerolPSynthase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854:SF2">
        <property type="entry name" value="INDOLE-3-GLYCEROL-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22854">
        <property type="entry name" value="TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00218">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00614">
        <property type="entry name" value="IGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="10 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Indole-3-glycerol phosphate synthase" evidence="12">
        <location>
            <begin position="7"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001633"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012362"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00018080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96019.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="215" mass="24155" checksum="AFA0A6414B8D3E7B" modified="2013-06-26" version="1">MNKNYLNPIIAEIKPSSPSKGKIKEINENNIKDIAKELIEGNATALSVLTEPKYFNGSYKNIILLRDINIPILMKDFVIDKYQIEIANEIGADAVLLIVSALGEELYKFLDYVDDYNLEALVEVSNEEEIDLAVDAGAKIIGINNRDLRTLNIDLKRTEMLSKFIPKGKIKVSESGVKSREDLLYVLKYTDAALVGTSIMESDNIKAKMLWLTKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUS6</accession>
    <name>N6UUS6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">HisA/hisF family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_04448</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96089.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591810.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUS6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00634"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.872"/>
    <dbReference type="OrthoDB" id="146815at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04723">
        <property type="entry name" value="HisA_HisF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004650">
        <property type="entry name" value="HisA/F-archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00734">
        <property type="entry name" value="hisAF_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090:SF2">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96089.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="219" mass="24699" checksum="D28AED9BE89D3418" modified="2013-06-26" version="1">MEVIPVIDIKDGKAVSGKSGKRDDYKPLKSIFCPIPNPIKISEALSEYFNRIYIADLNAIMGRGNNFDIIKDIKMKKIVDFGIRRREDFNICKKFLKENDKIILATETLKDLSLLKENVFVSLDFKDNKLLNYDLDFIISLINEKTPLIILDISSVGTKRGINKELINMVMRKVNNPIYVGGGVRNKEDLKVCVDLGIDGVLVGTALHSGELKLDELLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV09</accession>
    <name>N6UV09_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96199.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591249.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV09"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01385"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.701"/>
    <dbReference type="OrthoDB" id="65656at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96199.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="52" mass="5948" checksum="F9411CD96D2F8391" modified="2013-06-26" version="1">MENKLENMGGLEEIEEIKKDITRPYTGSKSSYIMGYFLAILLIVATIIHILF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV18</accession>
    <name>N6UV18_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Hydrogenase isoenzymes formation protein HypD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_03653</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HypD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96209.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591268.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV18"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01374"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.713"/>
    <dbReference type="OrthoDB" id="372075at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070025">
        <property type="term" value="F:carbon monoxide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11750">
        <property type="entry name" value="HypD, alpha/beta domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002780">
        <property type="entry name" value="Hyd_form_HypD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042243">
        <property type="entry name" value="HypD_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042244">
        <property type="entry name" value="HypD_2_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00075">
        <property type="entry name" value="hypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30149:SF0">
        <property type="entry name" value="HYDROGENASE MATURATION FACTOR HYPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30149">
        <property type="entry name" value="HYDROGENASE PROTEIN ASSEMBLY PROTEIN HYPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01924">
        <property type="entry name" value="HypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005622">
        <property type="entry name" value="Hydrgn_mat_hypD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96209.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="354" mass="40241" checksum="C89EDCAA0DD1431A" modified="2013-06-26" version="1">MIKKIIEKIHDLSRDVGDIKIMHVCGSHEHTICKYGIRDVLPNNISLIPGPGCPVCVTTQKEIDTAIYLSENYSIITLGDLYRVPGSYKSLAEAKAEGKDIRIVYSIADAVRIAKKEKDKNFLFVAIGFETTAPTTAAELISLKNRDINNFFILNCHRQIPPVMEFLLEDSKIDAFICPGHVSTIIGLKPYYKLLEKYKVPMVVAGFEAIDILLSILMILRQIVKNDIKVENEYKRAVRKEGNRIAQKVISKVFEDEDKPWRGFPIIKNGGYKLKEEFKKFDIFEVEDIPEIKERIPKGCICDKILRGEKLPTDCPLFGKKCTPLNPIGSCMVSEEGTCRIFYKYKFTFSHHSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV38</accession>
    <name>N6UV38_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glycosyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03493</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000022">
        <property type="protein sequence ID" value="ENN96229.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591150.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV38"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01335"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.681"/>
    <dbReference type="OrthoDB" id="64615at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630:SF2">
        <property type="entry name" value="GLYCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43630">
        <property type="entry name" value="POLY-BETA-1,6-N-ACETYL-D-GLUCOSAMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="12"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96229.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="287" mass="33114" checksum="FA1DF986912E2C43" modified="2013-06-26" version="1">MSLSKEECPLVSVVIPTYNSEKTIGICLESIKNQTYKNIEIIVVDKFSDDNTVKIAKKYTDKVFVINAKERSEQLNFGVKMAKGKYIYRVDSDFVLEPTVIEEAVKKCEEEGYDAVCIHNTSDPTISFWAKVRKLERDCYEDDKLNVAARFIRKDAFENVGGFDENLVAAEDYDLHNRLLRAGYKIGRIKSKEIHIGEPKSLWEIIKKHYYYGKTLPKFVEKNKGRGIKQLSPIRPAYLKHWKKFVKHPVLTLGFIIYQISRYGAAGIGYIVGKMKFIFFNRANNVD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV44</accession>
    <name>N6UV44_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">tRNA (cytidine(56)-2'-O)-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.206</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">tRNA ribose 2'-O-methyltransferase aTrm56</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">J422_03518</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Specifically catalyzes the AdoMet-dependent 2'-O-ribose methylation of cytidine at position 56 in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 16">
            <text>cytidine(56) in tRNA + S-adenosyl-L-methionine = 2'-O-methylcytidine(56) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:42968"/>
            <dbReference type="Rhea" id="RHEA-COMP:10308"/>
            <dbReference type="Rhea" id="RHEA-COMP:10309"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:74495"/>
            <dbReference type="ChEBI" id="CHEBI:82748"/>
            <dbReference type="EC" id="2.1.1.206"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the aTrm56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.206" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000022">
        <property type="protein sequence ID" value="ENN96234.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591157.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV44"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01330"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.686"/>
    <dbReference type="OrthoDB" id="14397at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106059">
        <property type="term" value="F:tRNA (cytidine(56)-2'-O)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002128">
        <property type="term" value="P:tRNA nucleoside ribose methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002845">
        <property type="entry name" value="tRNA_mtfrase_aTrm56"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42197">
        <property type="entry name" value="TRNA (CYTIDINE(56)-2'-O)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42197:SF1">
        <property type="entry name" value="TRNA (CYTIDINE(56)-2'-O)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01994">
        <property type="entry name" value="Trm56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016123">
        <property type="entry name" value="UCP016123"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 14" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <keyword evidence="11" id="KW-0819">tRNA processing</keyword>
    <feature type="non-terminal residue" evidence="14">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010324"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00013709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047792"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN96234.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="147" mass="17017" checksum="1128B9B4702770E9" modified="2013-06-26" version="1" fragment="single">AEKIVFTVEDDHVRESVEKVVKTWGGNFKFTVIKNWRNYIKEFKKKGGIVVHLTMYGANVNEIMPEIKKLNRDILIIVGAEKVPRDVYELADYNISIGNQPHSEVAALAIFLDRLFEGKTLYREFDNAKMKIIPSLKEKIVLKENKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV70</accession>
    <name>N6UV70_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">tRNA (Adenine-N(1)-)-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_03323</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96259.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590964.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV70"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01051"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.651"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031515">
        <property type="term" value="C:tRNA (m1A) methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160107">
        <property type="term" value="F:tRNA (adenine(58)-N1)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049470">
        <property type="entry name" value="TRM61_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014816">
        <property type="entry name" value="tRNA_MeTrfase_Gcd14"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12133:SF1">
        <property type="entry name" value="TRNA (ADENINE(58)-N(1))-METHYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08704">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017269">
        <property type="entry name" value="GCD14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51620">
        <property type="entry name" value="SAM_TRM61"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRM61 C-terminal" evidence="6">
        <location>
            <begin position="66"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="167"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR017269-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF08704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96259.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="269" mass="30635" checksum="FC84EACF1BB41F6F" modified="2013-06-26" version="1">MDYKLLVDERGKKYLLKKSVEKFCTDLGVVETKEIGKRLESHLGHKFYLVEPTLYDVLKRMKRTVTALLPKDIGFIIARGGIREGETIVEAGTGAGVLTMYLANAVGRGGKVISYDIKPEAIKVARKNLLRVGLIKKNQKILGLDEDFEDDIEVEDGLFNVILKFGDVREKIDEKNVDVIVLDMPDPWNVIENAKEVLNKKRGRIITYLPYIDQVKKTVSKLKEEGFWDIETYELMERMIEVSDKGVRPSTRMIGHTGYITFARAPLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVI2</accession>
    <name>N6UVI2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">1-(5-phosphoribosyl)-5-amino-4-imidazole-carboxylate (AIR) carboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02724</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96359.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590598.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVI2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00097"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.534"/>
    <dbReference type="OrthoDB" id="372165at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039476">
        <property type="entry name" value="P2CMN_synthase_LarB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033503">
        <property type="entry name" value="LarB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43064">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43064:SF1">
        <property type="entry name" value="SLL1489 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurE" evidence="1">
        <location>
            <begin position="123"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96359.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="261" mass="28757" checksum="DA2B50D1BFCC7820" modified="2013-06-26" version="1">MDLKKILLMFKEGKISLEEAEKFIKLNYYENLEGILRLDVNRKFRTGIPEVVFGRGKELEDIIKATLKLAERNNIALATKIDNINELEKKIKEYDLKGYIVKINKKAKTLTIIKEGYKVKKIGKVAILTGGTADIPIAEEAKETLNILGVEAITFYDVGVAGIHRLFPALKIILEEKIPCAIVIAGMEGALPSVVASLIDIPIIAVPTSVNYGVKITPLLAMLHSCSPGVAVVNIDNGFGAATFAYLIVSVMNRWKHDKNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVL5</accession>
    <name>N6UVL5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02914</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96389.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590659.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVL5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00131"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.572"/>
    <dbReference type="OrthoDB" id="73420at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07741">
        <property type="entry name" value="metallo-hydrolase-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42663:SF6">
        <property type="entry name" value="HYDROLASE C777.06C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42663">
        <property type="entry name" value="HYDROLASE C777.06C-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="37"/>
            <end position="222"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96389.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="269" mass="30376" checksum="A41F09BF573FE824" modified="2013-06-26" version="1">MRVEIIFLSSGGGRWATITQKKATGGFRIHTEKLKMHIDPGPGAVVRMSQIKINPWNTNTLFISHCHPDHYTDGEIIVEAITYGMTKKRGVFIGSLTAVEGFGEYERVISNYHKSKLELAKPLYPGEEIKIYDCKIKATHTKHGDPFGIGFRLETVFGEIGYTSDTEFFPKLIEDFDGVRILIANIVREKNKKVRGHLNSNNAIDLINSMSKKPELLIMNHMGLNMKNPKKEAEYISENTGIDVIPAELGLKIELLNGKYIYKLKKVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVT4</accession>
    <name>N6UVT4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rps6e</name>
        <name evidence="5" type="ORF">J422_02105</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96444.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVT4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01170"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.414"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00512">
        <property type="entry name" value="Ribosomal_eS6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001377">
        <property type="entry name" value="Ribosomal_eS6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020924">
        <property type="entry name" value="Ribosomal_eS6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018282">
        <property type="entry name" value="Ribosomal_eS6_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003294">
        <property type="entry name" value="PRK04290.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11502">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01092">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01405">
        <property type="entry name" value="Ribosomal_S6e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00578">
        <property type="entry name" value="RIBOSOMAL_S6E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00512"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96444.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="142" mass="15553" checksum="88010DB6A96CC74E" modified="2013-06-26" version="1">MRLLKPLGIEVKGEIMPIAKFVVADPKTGRCYQIEADNTPLVGKKIGDIFDGSIIGLNGYKLQITGGTDDSGFPMRPDIHGSGKVRVLLSGPPGFWPKRKGERRRKTVRGNTIAPDIVQINCKVIEYGEKPIPEILGLESKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWE1</accession>
    <name>N6UWE1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">N-acetylglucosamine-1-phosphate transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_01278</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96629.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589919.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWE1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00034"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.250"/>
    <dbReference type="OrthoDB" id="34534at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016780">
        <property type="term" value="F:phosphotransferase activity, for other substituted phosphate groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044038">
        <property type="term" value="P:cell wall macromolecule biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071555">
        <property type="term" value="P:cell wall organization"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000715">
        <property type="entry name" value="Glycosyl_transferase_4"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926">
        <property type="entry name" value="PHOSPHO-N-ACETYLMURAMOYL-PENTAPEPTIDE-TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22926:SF3">
        <property type="entry name" value="UNDECAPRENYL-PHOSPHATE ALPHA-N-ACETYLGLUCOSAMINYL 1-PHOSPHATE TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00953">
        <property type="entry name" value="Glycos_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="48"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="83"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="133"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="187"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="265"/>
            <end position="287"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96629.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="292" mass="33370" checksum="7582EBF728D55263" modified="2013-06-26" version="1">MEIFAFLFSLGLTYFLIKRINMFGHDLHKKDKKKIPEMGGIAPLLSNILFIPFIKVFYIIPIVFSGLLGIIDDISKLKPKEKLIFLFIFSLIFGYFLYINGFISLWEVLLLAVSITVFSNLTNMLAGFNGLEIGLGIISSFFLAIFCYIYGYYDAYHALLIFSASYFGLFIYNKYPAKVFPGDVGTLPIGAFLSTLAVIYHQYIPFLIIMTPYIVDASLKFITAGVMSRDEHKPTVLGEDNKLYYISGYLSLPRLILKFKPMKEWQLVVVLYIIEIIFCIIALIFVINSKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6UZY4</accession>
    <name>N6UZY4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_07037</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000068">
        <property type="protein sequence ID" value="ENN95588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594743.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UZY4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00917"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1363"/>
    <dbReference type="OrthoDB" id="63460at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="101" mass="12095" checksum="7B5EF00CCA8181FC" modified="2013-06-26" version="1">MRAKEIWKELLKIAKDYYDEDKKFYSKTKRGVYKIKGFTKDKIIIKKLSGKLDDVLTKKRFINLFDELLYGADVKPISIKSFLKLHPKIEEENNKLYYRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6V010</accession>
    <name>N6V010_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase</fullName>
            <ecNumber evidence="9">4.1.3.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">tRNA wyosine derivatives biosynthesis protein Taw1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">taw1</name>
        <name evidence="11" type="ORF">J422_06872</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the wyosine derivatives biosynthesis pathway that catalyzes the condensation of N-methylguanine with 2 carbon atoms from pyruvate to form the tricyclic 4-demethylwyosine (imG-14) on guanosine-37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>N(1)-methylguanosine(37) in tRNA(Phe) + pyruvate + S-adenosyl-L-methionine = 4-demethylwyosine(37) in tRNA(Phe) + 5'-deoxyadenosine + L-methionine + CO2 + H2O</text>
            <dbReference type="Rhea" id="RHEA:36347"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10165"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73542"/>
            <dbReference type="EC" id="4.1.3.44"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the TYW1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.44" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594253.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V010"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01073"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1333"/>
    <dbReference type="OrthoDB" id="68499at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102521">
        <property type="term" value="F:tRNA-4-demethylwyosine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01921">
        <property type="entry name" value="TYW1_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013917">
        <property type="entry name" value="tRNA_wybutosine-synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034556">
        <property type="entry name" value="tRNA_wybutosine-synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023993">
        <property type="entry name" value="TYW1_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03972">
        <property type="entry name" value="rSAM_TYW1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13930:SF0">
        <property type="entry name" value="S-ADENOSYL-L-METHIONINE-DEPENDENT TRNA 4-DEMETHYLWYOSINE SYNTHASE TYW1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08608">
        <property type="entry name" value="Wyosine_form"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00284">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01071">
        <property type="entry name" value="tRNA_wybutosine-synthesizing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0408">Iron</keyword>
    <keyword evidence="6 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 9" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 9" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Radical SAM core" evidence="10">
        <location>
            <begin position="42"/>
            <end position="287"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00049466"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="306" mass="36318" checksum="66C490E897384A0D" modified="2013-06-26" version="1">MKMIELLRKQRYQIDNHTAVKLCGWVRKKMLEDKNCYKSKFYGIETHRCIQCSPAFMWCQQSCIFCWRVLPSDIDAKIEEPNWEEPEVVYEKILKLHRRAIMGYKGILNRVGEKKFNEALNPKHVAISLSGEPTLYPYLDELIKIFHKNGFTTFVVSNGILTDVIEKIEPTQLYISLDAYDLESYKKICRGKEEYWENILETLDILKKKKRTCIRTTLVRGYNDDILKFVELYERADVHFIELKSYMHVGYSQRRLEKKHMLKHDEILKLAKMLEEESKYKLIDDAEDSRVALLKNTDRDISVKIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6V047</accession>
    <name>N6V047_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06573</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000055">
        <property type="protein sequence ID" value="ENN95673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593721.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V047"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00604"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1279"/>
    <dbReference type="OrthoDB" id="15347at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="29"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="59" mass="6682" checksum="1669773C4B090FD3" modified="2013-06-26" version="1">MVRVNYKRCGYCGACVGVCKNLAITLEEFIIRIDREKCINCNSCVIVCPLNALQREENV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0C5</accession>
    <name>N6V0C5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 11 12">Methenyltetrahydromethanopterin cyclohydrolase</fullName>
            <ecNumber evidence="3 11 12">3.5.4.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">Methenyl-H4MPT cyclohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">mch</name>
        <name evidence="13" type="ORF">J422_06045</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the reversible interconversion of 5-formyl-H(4)MPT to methenyl-H(4)MPT(+).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2O = N(5)-formyl-5,6,7,8-tetrahydromethanopterin + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19053"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58018"/>
            <dbReference type="ChEBI" id="CHEBI:58337"/>
            <dbReference type="EC" id="3.5.4.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="11">One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 3/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the MCH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.27" evidence="3 11 12"/>
    <dbReference type="EMBL" id="APMM01000048">
        <property type="protein sequence ID" value="ENN95773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593152.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0C5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00929"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1177"/>
    <dbReference type="OrthoDB" id="105468at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00694"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018759">
        <property type="term" value="F:methenyltetrahydromethanopterin cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00545">
        <property type="entry name" value="MCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.340.11">
        <property type="entry name" value="Methenyltetrahydromethanopterin Cyclohydrolase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1030.10">
        <property type="entry name" value="Methenyltetrahydromethanopterin Cyclohydrolase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00486">
        <property type="entry name" value="McH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003209">
        <property type="entry name" value="METHMP_CycHdrlase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03120">
        <property type="entry name" value="one_C_mch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02289">
        <property type="entry name" value="MCH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56199">
        <property type="entry name" value="Methenyltetrahydromethanopterin cyclohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 11 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 11" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="6 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020597"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00030468"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00486"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR03120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN95773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="321" mass="35087" checksum="EA8C128A772D1557" modified="2013-06-26" version="1">MLSINKRACKIVKEMIAKKEELNIDVKKLENGTTVLDCGVNVKGSWEAGKRFTRVCLGGLAKVGISLQVNKELNLPYVKVKTSHPAIATLASQKAGWAIKVGKYFAMGSGPARALAKKPKKTFEEIGYEDNADVAVLCLESSKLPNEEVADYIAKECNVEPENLYLLVAPTASLVGSIQISGRVVENGTYKMLEVLGFDVNKVEFAAGLAPIAPIVGDDFTMMGATNDMVMYGGIVYYYIKSDDDDLLSLCKALPSCSSKDYGKPFLEIFKAADYDFYKIDKGIFAPAKVIINDISKGKVYEAGEVNYEIIKKSLNWESVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0E3</accession>
    <name>N6V0E3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_05908</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000046">
        <property type="protein sequence ID" value="ENN95793.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592964.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0E3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00990"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1152"/>
    <dbReference type="OrthoDB" id="64496at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="5"/>
            <end position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95793.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="299" mass="35140" checksum="3F2F253DE6CB0808" modified="2013-06-26" version="1">MSKTILLTGATGFLGSHLLESLLKENYNVIILKRSFSNTWRIQHLLDNVIYYDIDKVELEKPFKENQIDVVIHTATSYGRKNEKISEIVDTNLMFPLKLLELCTFFNIDTFFNTDTILPKNLNYYVLSKKQFLEYGKRMCDEYNLRFINIKLEHMYGPKDDNTKFIPYIIEKILKNEKEIDLTKGEQERDFIYISDVVSAYITVLNKLDNFEKKFYNIEVGVGNPIKIRDLVILIKKLCNSNIKLNFGAIPYRKNEIMKSYANIDFLKSLGWEPKISIEEGLKKTIDYYKKLMVEKNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0R4</accession>
    <name>N6V0R4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05344</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95903.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592382.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0R4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01318"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1043"/>
    <dbReference type="OrthoDB" id="64462at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014515">
        <property type="entry name" value="UCP921964"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09893">
        <property type="entry name" value="DUF2120"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF021964">
        <property type="entry name" value="UCP921964"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95903.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="140" mass="16407" checksum="341F49D4A5BCE0AD" modified="2013-06-26" version="1">MIWKANIGKLMHAFKAFRGSKPIFENDKLLIVKSVCKDEEIDNYENLKDYIIKKLKNEGYDIIENPEEIDEFVDKINENFMKEKDITPFGFEKMKESFKIIGCECDYAIGKKNNIMVGVTIYYDKNMKKPKFIEVVGLCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0W6</accession>
    <name>N6V0W6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0254 protein J422_04880</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04880</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0254 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95948.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592068.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0W6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00515"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.956"/>
    <dbReference type="OrthoDB" id="59686at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00673">
        <property type="entry name" value="UPF0254"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009625">
        <property type="entry name" value="HcgF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002122">
        <property type="entry name" value="PRK00962.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06787">
        <property type="entry name" value="HcgF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95948.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="168" mass="18975" checksum="C648F8BE61BB3AD9" modified="2013-06-26" version="1">MITLATAECFTHGKIGLSIHKAASGYEEFEYKYLFSEEDLKIVKNVKVLCSMFLPSIYAIEKLFNISLPKPDYYYKYAKAYSEKNDLAVAKKIAESLKDKLNPDIALASTAGVGRGAICIIADKCYLFTTDVYANLLTFENVKERQKDGIEKGIKKVLEIVKKCLEKY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6V195</accession>
    <name>N6V195_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04233</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96048.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591724.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V195"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00676"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.828"/>
    <dbReference type="OrthoDB" id="65909at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96048.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="103" mass="12082" checksum="AF57FAEC9E35F628" modified="2013-06-26" version="1">MKLAIDGVFYVREGFDFEKVFKEILTMLKDVRILSVEYPELALIAPDGYYYRCGFMLDKPLEEKISEEEIKEIKRKIEELFKGEIIYTLTCEILGEEDEGNSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1F9</accession>
    <name>N6V1F9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0285 protein J422_04503</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0285 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96098.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591830.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1F9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00624"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.883"/>
    <dbReference type="OrthoDB" id="235676at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01087">
        <property type="entry name" value="UPF0285"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016735">
        <property type="entry name" value="Methan_mark_12"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03281">
        <property type="entry name" value="methan_mark_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018783">
        <property type="entry name" value="UCP018783"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01087"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96098.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="325" mass="35711" checksum="0680DACC246D3613" modified="2013-06-26" version="1">MITVGIDHGTSGITTCIKEGSKKIIFKLKRSEVKERSYLEELKRYVDLDKIDMITLTYSMGDGINKILPIDKVKNRGVKSLEGAGEKIGGGTKVFDEIKESSLPAIVLPGLHRDIECLDERFRALFSHIASPEKISIAYYAYKLFKFNNFVLSDISSNTVTLLIKNGKFFGGFDACIGAIGILHGPIDLEMIRKIDNNEISANEAFSKAGAVKIAKIYKGPEETKDEIIKNYYTNKNCKLAIDSLILSVSLEINSLLSLTDKKRVVLAGSVGTLRKPIDIPKEIKKFVKAKIHVLYGESGAIGGALIGEDVLKGVDEILGIKVEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1N4</accession>
    <name>N6V1N4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03588</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96198.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591247.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1N4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01386"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.700"/>
    <dbReference type="OrthoDB" id="6433at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07709">
        <property type="entry name" value="flavodiiron_proteins_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008254">
        <property type="entry name" value="Flavodoxin/NO_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001226">
        <property type="entry name" value="Flavodoxin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045761">
        <property type="entry name" value="ODP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016440">
        <property type="entry name" value="Rubredoxin-O_OxRdtase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43717">
        <property type="entry name" value="ANAEROBIC NITRIC OXIDE REDUCTASE FLAVORUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43717:SF1">
        <property type="entry name" value="ANAEROBIC NITRIC OXIDE REDUCTASE FLAVORUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00258">
        <property type="entry name" value="Flavodoxin_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19583">
        <property type="entry name" value="ODP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005243">
        <property type="entry name" value="ROO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00201">
        <property type="entry name" value="FLAVODOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50902">
        <property type="entry name" value="FLAVODOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavodoxin-like" evidence="1">
        <location>
            <begin position="245"/>
            <end position="384"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50902"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96198.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="386" mass="44544" checksum="741172494D141902" modified="2013-06-26" version="1">MAIKVKDGIYWLGTVDWESRDFHGYPVSSGTSYNSYLIVDEKKVLIDTTECHLYNELMKNLSTIIDPKDLDYIVLNHTEKDHSEALPKIVEKTNATIITNANAKEYLSLYYNTEGWDFIIVEDGDEIEIGKRTLTFIKTPMLHWPDNMMVYTDNTLFSNDAFGQHIATNKIFDYQYGKKALNAAKEYFATILMPYRKLIPNAIEKIKDLEIDYICPSHGVIWKEYKEDIIKSYLDWSNDKVKEKAVIVYDTMYGSTKKIAHALANAFIDLGIEVKVYRISTSSISEILAEIVDAKYLLIGSPTMNMDIYPEVSKFLNYLKNLRVGNKISAVFGSYGWREGASDKIKNILKELNFKIVDDECLRVRFTPKDEELNKIIRFVKDIVEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6V205</accession>
    <name>N6V205_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="3">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">J422_03124</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96318.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V205"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00171"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.613"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd05144">
        <property type="entry name" value="RIO2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.200.20:FF:000052">
        <property type="entry name" value="Serine/threonine-protein kinase RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000719">
        <property type="entry name" value="Prot_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030484">
        <property type="entry name" value="Rio2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015285">
        <property type="entry name" value="RIO2_wHTH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852">
        <property type="entry name" value="SER/THR-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45852:SF1">
        <property type="entry name" value="SERINE_THREONINE-PROTEIN KINASE RIO2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09202">
        <property type="entry name" value="Rio2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50011">
        <property type="entry name" value="PROTEIN_KINASE_DOM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 14" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Protein kinase" evidence="13">
        <location>
            <begin position="65"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50011"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN96318.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="266" mass="31231" checksum="0AD69A73B9E9EFE0" modified="2013-06-26" version="1">MRHHEWVPIDEIVKKAKMPEKDVLYRLKRLNKFEFVVRSIYGYAVSFGGYDALAIDALYKKGILKAIGNKLGVGKEGDVYNVLLSDDREAVLKFHKHGRTCFTRGKRYREFVSNKHHISWLYMSRLTAEREFEILNELFPTVKVPEPIAQNRHAIIMGKITGEELKVLDLEKLMSKEEIEELFNKIIEEIKKAYKIGYIHGDLSEFNILLDENLDFVIIDWPQAITKGHPDAEFYLKRDINNIVRYFKKYKIKADPDEIYKYVVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6V218</accession>
    <name>N6V218_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2118 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03174</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96328.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590797.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V218"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00181"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.623"/>
    <dbReference type="OrthoDB" id="84532at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.128.400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019217">
        <property type="entry name" value="DUF2118"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09891">
        <property type="entry name" value="DUF2118"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96328.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="158" mass="18432" checksum="6A0D208F0742BF4E" modified="2013-06-26" version="1">MRFPKLFVENPKKPEKDRVVVERDGKVIKFLEEGEEYSGDGKVLYEVIYDDFDEYVLMGTVVCDMLMYYEVGEVKQITYIKKGTKLLEIPAEGYKVYPIVDFGCRVLEGHRLAALQSRKGDIRFVNTPETGIVLFLKEVPSKRENYIFYILPEGKKWD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6V226</accession>
    <name>N6V226_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_02619</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96338.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590555.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V226"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00076"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.513"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051685">
        <property type="entry name" value="Ycf3/AcsC/BcsC/TPR_MFPF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943">
        <property type="entry name" value="CELLULOSE SYNTHASE OPERON PROTEIN C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44943:SF8">
        <property type="entry name" value="TPR REPEAT-CONTAINING PROTEIN MJ0263"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13424">
        <property type="entry name" value="TPR_12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14559">
        <property type="entry name" value="TPR_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="43"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="145"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="248"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="282"/>
            <end position="315"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96338.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="319" mass="36998" checksum="82E04C5740EF2C97" modified="2013-06-26" version="1">MPQIKELDSILNILYAWRNLSEGNSLEALYYINKALETNPDNTIALFLKGIILLGMGDVEGSKETLEKLVKLSPKNYMALSLLSLVNSLLGDFKKSIELNEEALSIESQFLMGWLLKIINLDYLYDYDELLKIYKKFSKKCPKFSIIDIKSADILRKYGRYHEALEFLDKALSLNPENIGAIYLKGVILKRLGRYEEALECFKKLIDEMNIRWLDAIRHAASTCLVLDKLDDAERYVNMGLKIKNRDISLWYFKALIHEYRGNLDEALKCFQKVVNIYPNHVKAYLSMAKIYEKKGDYDKAIKYYETALLKRKEDLRGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6V232</accession>
    <name>N6V232_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Type 2 DNA topoisomerase 6 subunit A</fullName>
            <ecNumber evidence="11">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Type II DNA topoisomerase VI subunit A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">top6A</name>
        <name evidence="16" type="ORF">J422_02644</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="18">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11 12">Belongs to the TOP6A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96343.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590565.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V232"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00081"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.518"/>
    <dbReference type="OrthoDB" id="5866at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00223">
        <property type="entry name" value="TOPRIM_TopoIIB_SPO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00132">
        <property type="entry name" value="Top6A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002815">
        <property type="entry name" value="Spo11/TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013049">
        <property type="entry name" value="Spo11/TopoVI_A_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036078">
        <property type="entry name" value="Spo11/TopoVI_A_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049333">
        <property type="entry name" value="Topo_VI_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004085">
        <property type="entry name" value="TopoVI_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034136">
        <property type="entry name" value="TOPRIM_Topo6A/Spo11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003337">
        <property type="entry name" value="PRK04342.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10848:SF0">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN SPO11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21180">
        <property type="entry name" value="TOP6A-Spo11_Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20768">
        <property type="entry name" value="Topo_VI_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04406">
        <property type="entry name" value="TP6A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01550">
        <property type="entry name" value="TOP6AFAMILY"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01552">
        <property type="entry name" value="TPISMRASE6A"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56726">
        <property type="entry name" value="DNA topoisomerase IV, alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52041">
        <property type="entry name" value="TOPO_IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9 11 12" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10 11 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11 12" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Spo11/DNA topoisomerase VI subunit A N-terminal" evidence="13">
        <location>
            <begin position="76"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="Type II DNA topoisomerase VI subunit A all-beta" evidence="14">
        <location>
            <begin position="142"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="domain" description="Topoisomerase 6 subunit A/Spo11 TOPRIM" evidence="15">
        <location>
            <begin position="192"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="active site" description="O-(5'-phospho-DNA)-tyrosine intermediate" evidence="11 12">
        <location>
            <position position="103"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="197"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01385"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF20768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN96343.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="369" mass="41818" checksum="CAC6B311DC4DB6A9" modified="2013-06-26" version="1">MANIPKIPKKPREIAKQKIMELAKKMYEDLKKGIRPKIKMPIRSLSNALFDRDKGSFTLAGKEKTRTLTVSQAKIFAQTAKMLELAKQLLDTDDFATLREAYYVSKNWGEARFDDQQVSNNVIEDLEAALGVLREHLGFIPEEDGSAVVGPLKIIEETSEGELVVDCRKLGTGAYNIPNDVTKLNLETNADFVLAIETSGMFARLNAERFWDKHNCILVSLKGVPARATRRFIKRLNEEFDLPVLVFTDGDPYGYLNIYRTLKVGSGKSVHLADKLSIPDARLIGVTPQDIIDYDLPTHPLKEQDIKRIKDGLNNDDFVRSFPKWQEALKQMLKMGVRAEQQSLAKYGLKYVVNTYLPEKIKDDSTWLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6V282</accession>
    <name>N6V282_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NUDIX hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02874</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96383.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590646.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V282"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00124"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.564"/>
    <dbReference type="OrthoDB" id="40462at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18873">
        <property type="entry name" value="NUDIX_NadM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.79.10">
        <property type="entry name" value="Nucleoside Triphosphate Pyrophosphohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020476">
        <property type="entry name" value="Nudix_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015797">
        <property type="entry name" value="NUDIX_hydrolase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020084">
        <property type="entry name" value="NUDIX_hydrolase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000086">
        <property type="entry name" value="NUDIX_hydrolase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43736">
        <property type="entry name" value="ADP-RIBOSE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43736:SF1">
        <property type="entry name" value="DIHYDRONEOPTERIN TRIPHOSPHATE DIPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00293">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00502">
        <property type="entry name" value="NUDIXFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55811">
        <property type="entry name" value="Nudix"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51462">
        <property type="entry name" value="NUDIX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00893">
        <property type="entry name" value="NUDIX_BOX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nudix hydrolase" evidence="2">
        <location>
            <begin position="36"/>
            <end position="165"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96383.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="167" mass="19000" checksum="6737EBA281D1DD6C" modified="2013-06-26" version="1">MKCLSISGKLIALNLFGKRYCKKIIRKRDLKRYKLYTTPAIAVDGIVVKDNKILLIKRKNDPFKGYYALPGGFVECGETVENAIIREVREETGLEVEVKRLFGVYSSPNRDPRGHVISIVFILEIVGGKLKAGDDAKDVKFFELNNLPKLAFDHNNILEDFLSHKFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2K3</accession>
    <name>N6V2K3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Ribosomal L11 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01665</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96488.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590093.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2K3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00185"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.327"/>
    <dbReference type="OrthoDB" id="31271at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051720">
        <property type="entry name" value="rRNA_MeTrfase/Polyamine_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290:SF0">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96488.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="207" mass="24035" checksum="3946467BFB30D396" modified="2013-06-26" version="1">MKMKHLEMILDSLKTHTKKPELEQYTIPGKLASEILFFAKDDFYNNIALDFGCGSGRLAIGCKLLGAKRVIGIDIDKEAIEVAKKNAEYLNLDIDFYCMDIREVDEEFLNAILKEDKDLKRVVIQNPPFGAQKRHADRIFIDKALELGDVIYTIHNYPTKDFIINYVKGRGRITHIYETNFKIPAIYKFHKKRLVEIPVVIFRIERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2M3</accession>
    <name>N6V2M3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01775</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96508.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590114.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2M3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00206"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.349"/>
    <dbReference type="OrthoDB" id="65974at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96508.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="174" mass="20427" checksum="DEA7F377756AF611" modified="2013-06-26" version="1">MTYPTLVEIKLEKDKLFEIGENKVNELLKIRTKLETLRKNNGDIDEIIALEDKENQLISEISKIDLMIKILEIVEFIIESGLFEKYLDILEKNIEYDELLDIVVKNNLCVKKTCLEIYKRLGLNDKNILKNIEALEECEEDHEEPTYIKNIIRRINNLKSKICDEGNNEKGRES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2X1</accession>
    <name>N6V2X1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">RNA-associated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_01585</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SDO1/SBDS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2X1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00444"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.312"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042256">
        <property type="term" value="P:cytosolic ribosome assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1250.10">
        <property type="entry name" value="Ribosome maturation protein SBDS, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.900">
        <property type="entry name" value="SBDS protein C-terminal domain, subdomain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035647">
        <property type="entry name" value="EFG_III/V"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036786">
        <property type="entry name" value="Ribosome_mat_SBDS_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002140">
        <property type="entry name" value="Sdo1/SBDS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039100">
        <property type="entry name" value="Sdo1/SBDS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046928">
        <property type="entry name" value="SDO1/SBDS_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018978">
        <property type="entry name" value="SDO1/SBDS_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037188">
        <property type="entry name" value="Sdo1/SBDS_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019783">
        <property type="entry name" value="SDO1/SBDS_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00291">
        <property type="entry name" value="RNA_SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SBDS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10927:SF4">
        <property type="entry name" value="RIBOSOME MATURATION PROTEIN SDO1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20268">
        <property type="entry name" value="SBDS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09377">
        <property type="entry name" value="SBDS_domain_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01172">
        <property type="entry name" value="SBDS_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54980">
        <property type="entry name" value="EF-G C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89895">
        <property type="entry name" value="FYSH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109728">
        <property type="entry name" value="Hypothetical protein AF0491, middle domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS N-terminal" evidence="2">
        <location>
            <begin position="7"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS central" evidence="3">
        <location>
            <begin position="101"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Ribosome maturation protein SDO1/SBDS C-terminal" evidence="4">
        <location>
            <begin position="166"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007433"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF09377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF20268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="208" mass="23556" checksum="8EBFDA8A118BBD06" modified="2013-06-26" version="1">MVSLEEAVIARLTAHGEKFEILVDPYLAAKLKEGLNVDMDELLAIDVVFKDANKGEKAPEELLMKVFGTTDVKEIAKKIILKGHVQLTAKQREEIREQKKKQIITIICKNTINPQTDTPHPPHRIEKALEELKINIDIYKSAEEQVPEIIKKLKRVLPIKFEKRDIAVKIPPEFAGKAYNVLHHFGSVKQEEWANDGSLIVLIEIPSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2Z2</accession>
    <name>N6V2Z2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Putative signal transduction protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_01343</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96603.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589932.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2Z2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00020"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.263"/>
    <dbReference type="OrthoDB" id="64432at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd02205">
        <property type="entry name" value="CBS_pair_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="10"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="5">
        <location>
            <begin position="76"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="4">
        <location>
            <begin position="150"/>
            <end position="171"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="4">
        <location>
            <begin position="150"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96603.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="171" mass="19333" checksum="D9AAB634AF4DDBEF" modified="2013-06-26" version="1">MIDDVPVVIAMSEPVFVRDNTSIYNTLKEMLKKNKKYCIVINSNNDIVGVATFSDIIKAIILEKRDGEKTSIKDVADSKVITVKPTTSIKEVLELMEKEKISKLPVVDNNKIVGIITKEELVDILPYIIGPLKDLVEYLLKIIDEELKSDDNKEEKKENKETVKVKAITTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6V303</accession>
    <name>N6V303_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Adenosylcobinamide amidohydrolase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01428</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V303"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00003"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.280"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002808">
        <property type="entry name" value="AdoCbi_amidolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052209">
        <property type="entry name" value="CbiZ"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35336">
        <property type="entry name" value="ADENOSYLCOBINAMIDE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35336:SF5">
        <property type="entry name" value="ADENOSYLCOBINAMIDE AMIDOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01955">
        <property type="entry name" value="CbiZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="255" mass="29065" checksum="A8A1D69A404E20C4" modified="2013-06-26" version="1">MLKMDDFEAKVVRHNVDVFGKVMEAKTLLVEFERRRRVLSTKEGFKNVKYVANHSLPDWRRIHRYNYKDYLKRVLDSLKIDEKDIAILTTAADMDNLAIAIEKFDEFYVVALTTAGAKDNAIRLGDEEADYIEKDFRTYKIEGDKLIPLEKYGTVNIIVITNADLTDGAMARAIITITEAKTNAFQELDVRSTKHPELLATGTGTDNVIVVKGFGRKVDYTGGHTKMGEMIAKAVKRSVIEALIKQDKIYKYKDF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6V365</accession>
    <name>N6V365_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Sm domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00976</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96698.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589863.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V365"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00580"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.192"/>
    <dbReference type="OrthoDB" id="63952at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005001">
        <property type="entry name" value="Hfq"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047575">
        <property type="entry name" value="Sm"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17209">
        <property type="entry name" value="Hfq"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS52002">
        <property type="entry name" value="SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Sm" evidence="1">
        <location>
            <begin position="16"/>
            <end position="74"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS52002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96698.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="74" mass="8880" checksum="FCA7291A96F807D8" modified="2013-06-26" version="1">MQKKQEQRRPKRPQQQNFEYVRRLNGKKVKIFLRNGEVLDAEITGISNYEIVVKVGERNLLIYKHAVDYIEFSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6V399</accession>
    <name>N6V399_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein eS27</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">rps27e</name>
        <name evidence="9" type="ORF">J422_00816</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7 8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the eukaryotic ribosomal protein eS27 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V399"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00872"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.161"/>
    <dbReference type="OrthoDB" id="5718at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.100:FF:000002">
        <property type="entry name" value="30S ribosomal protein S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.100">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00371">
        <property type="entry name" value="Ribosomal_eS27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000592">
        <property type="entry name" value="Ribosomal_eS27"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023407">
        <property type="entry name" value="Ribosomal_eS27_Zn-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001629">
        <property type="entry name" value="PRK00415.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11594">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01667">
        <property type="entry name" value="Ribosomal_S27e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01168">
        <property type="entry name" value="RIBOSOMAL_S27E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7 8" id="KW-0862">Zinc</keyword>
    <keyword evidence="3 7 8" id="KW-0863">Zinc-finger</keyword>
    <feature type="zinc finger region" description="C4-type" evidence="7">
        <location>
            <begin position="19"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="64" mass="7037" checksum="C9403C35083C9BC8" modified="2013-06-26" version="1">MDLAKIIPQPRSRFIKVQCPECNNEQIIFDRPATLVKCVVCGTVLAEPTGGKGKIKAKVLEVLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3C9</accession>
    <name>N6V3C9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00586</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3C9"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="34" mass="4019" checksum="12236D1547621B50" modified="2013-06-26" version="1" fragment="single">MFGKLKEKLFNAVSKISEKIYKKGEESEEEIKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3G9</accession>
    <name>N6V3G9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2098 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00125</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589714.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3G9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01143"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.25"/>
    <dbReference type="OrthoDB" id="52973at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019209">
        <property type="entry name" value="DUF2098"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017099">
        <property type="entry name" value="UCP037053"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09871">
        <property type="entry name" value="DUF2098"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037053">
        <property type="entry name" value="UCP037053"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9468" checksum="F8CE9559193FDF24" modified="2013-06-26" version="1">MNIKIGDYVMYINTGTKGKVVDIVEDYDGLWVVLDNGLMYRPHLLKVIDEKDVKKEEKKKIEIEEEEDIWEDKDYGDACGAG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VNZ1</accession>
    <name>N6VNZ1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Probable phosphoheptose isomerase</fullName>
            <ecNumber evidence="9">5.3.1.28</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Sedoheptulose 7-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">gmhA</name>
        <name evidence="11" type="ORF">J422_07187</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the isomerization of sedoheptulose 7-phosphate in D-glycero-D-manno-heptose 7-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>2 D-sedoheptulose 7-phosphate = D-glycero-alpha-D-manno-heptose 7-phosphate + D-glycero-beta-D-manno-heptose 7-phosphate</text>
            <dbReference type="Rhea" id="RHEA:27489"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:60203"/>
            <dbReference type="ChEBI" id="CHEBI:60204"/>
            <dbReference type="EC" id="5.3.1.28"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="9">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="9">Carbohydrate biosynthesis; D-glycero-D-manno-heptose 7-phosphate biosynthesis; D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate from sedoheptulose 7-phosphate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="9">The reaction produces a racemic mixture of D-glycero-alpha-D-manno-heptose 7-phosphate and D-glycero-beta-D-manno-heptose 7-phosphate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the SIS family. GmhA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.28" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000078">
        <property type="protein sequence ID" value="ENN95560.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004595167.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VNZ1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01008"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1388"/>
    <dbReference type="OrthoDB" id="64625at2157"/>
    <dbReference type="UniPathway" id="UPA00041">
        <property type="reaction ID" value="UER00436"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008968">
        <property type="term" value="F:D-sedoheptulose 7-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001061">
        <property type="term" value="P:D-glycero-D-manno-heptose 7-phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd05006">
        <property type="entry name" value="SIS_GmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00067">
        <property type="entry name" value="GmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035461">
        <property type="entry name" value="GmhA/DiaA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004515">
        <property type="entry name" value="Phosphoheptose_Isoase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050099">
        <property type="entry name" value="SIS_GmhA/DiaA_subfam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00441">
        <property type="entry name" value="gmhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30390:SF6">
        <property type="entry name" value="DNAA INITIATOR-ASSOCIATING PROTEIN DIAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30390">
        <property type="entry name" value="SEDOHEPTULOSE 7-PHOSPHATE ISOMERASE / DNAA INITIATOR-ASSOCIATING FACTOR FOR REPLICATION INITIATION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13580">
        <property type="entry name" value="SIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 9" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9 11" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="SIS" evidence="10">
        <location>
            <begin position="30"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="45"/>
            <end position="47"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="87"/>
            <end position="88"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="113"/>
            <end position="115"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95560.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="185" mass="20314" checksum="287BC7ED46CC48EF" modified="2013-06-26" version="1">MKEYFEESAKVKLKFIEENEENLKKSIEIIVRVLKNGNKILICGNGGSAADAQHFAAEIVGRFKLERKGLPAIALTTDTSILTAIGNDYGFEMIFERQVEALGKKGDVLVGISTSGNSKNVIRAVNKAKEMGIYTIGLLGKGGGKLKDIVDLALVVPSNDTARIQECHLTIYHVICEEVEKRLFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP24</accession>
    <name>N6VP24_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_07047</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000068">
        <property type="protein sequence ID" value="ENN95590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP24"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00915"/>
    <dbReference type="OrthoDB" id="65613at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="164" mass="19330" checksum="93A5E7599D4791B1" modified="2013-06-26" version="1" fragment="single">MKKYIKIGLIIFILINILPLIPFVVYLYENIGGAKDIGEVFKNSPFENFIYFDHNALMLMKSNNNSIYKELLIFINGIYIWEDNIAIKAPLTFIARYYPIDDFEYYNGLLIKCNNRSLLKKPKSDLINYVPKDYKDVVIYNGYSLIAIKYDVKDNKTLIYYVFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP32</accession>
    <name>N6VP32_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">DEAD/DEAH box helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06927</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000065">
        <property type="protein sequence ID" value="ENN95600.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP32"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0347">Helicase</keyword>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95600.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="96" mass="11395" checksum="02CAB62BF6F22674" modified="2013-06-26" version="1" fragment="single">MLIIRKIKKKDEIEIVKVENIDEGVEVRNSNKIFANYKKVGDRYKLYRCRLGDKLIQPSKVLELLKKEKIAIVKDKSLEELLKSYHLKFDYINLCP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP68</accession>
    <name>N6VP68_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0284 protein J422_06693</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06693</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0284 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000057">
        <property type="protein sequence ID" value="ENN95655.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593912.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP68"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00712"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1303"/>
    <dbReference type="OrthoDB" id="9136at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008939">
        <property type="term" value="F:nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02439">
        <property type="entry name" value="DMB-PRT_CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01086">
        <property type="entry name" value="UPF0284"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003200">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002805">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036087">
        <property type="entry name" value="Nict_dMeBzImd_PRibTrfase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00303">
        <property type="entry name" value="nicotinate mononucleotide-dependent phosphoribosyltransferase CobT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003372">
        <property type="entry name" value="PRK04447.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003374">
        <property type="entry name" value="PRK04447.1-7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38811:SF1">
        <property type="entry name" value="UPF0284 PROTEIN SLL1500"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02277">
        <property type="entry name" value="DBI_PRT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52733">
        <property type="entry name" value="Nicotinate mononucleotide:5,6-dimethylbenzimidazole phosphoribosyltransferase (CobT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95655.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="341" mass="37355" checksum="458BD6D3EE8BB75A" modified="2013-06-26" version="1">MIRINDNGFIEEIKKDALFICVISSIETTLHYPISGVHKDFIKYTPALDVELVFFGKTLTLKTPPVDSSFSPSPAVITRACVELKNIKNLHIDAGAFIKPKIPYIEIDKKPTGRIEEGKAMENSKLLYNKAYNLANNLDAELLIIGESVPGGTTTALGVLLGLGYDAEGKVSSGSINNPHELKLEIVRKGLKRAKSNNVFDILNAVGDKMMPVAAALAVNFLKRRKPVILAGGTQMAAVLAVIKEIYNDLDKIAIATTEFVINDKNSDLIGLVEQIADIPIYAAKFDFENSRLLGLRKYCEGAVKEGVGAGGVSVYSIANNLNPKRIREFIENNFKKWYGQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP80</accession>
    <name>N6VP80_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucose-methanol-choline oxidoreductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06593</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000055">
        <property type="protein sequence ID" value="ENN95675.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593725.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP80"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00601"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1283"/>
    <dbReference type="OrthoDB" id="346033at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="108"/>
            <end position="138"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95675.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="368" mass="41715" checksum="8C534D47C614271C" modified="2013-06-26" version="1">MYHYAIIGSGVAGATIAKELNDNVVILEKGDVAKYISEGDHVEVSYVCGLGGSGVFSLGNAMKVKLKGYKINKEIYKEIWEELKINVPNDDFLSKIDRELLSMGFKKMEKFIDFKKCNRCGKCAKKFCKAKWNPLNYLKGSKADIIKNFEIREIIREKDYFIINSKNRKIKAKNIIISAGGINSPRILRKLTKDDFGNGFFVDTFITVGGVLENSYLNENIPMLVYRDYKDFIISPHYTNLLYEEIKKENDVKEKDIMGLMIKIRDSLEGEILENDIKKEIAVNDIKLLAKGVSKATKFLNKLGIDEIYSTRARGSHPGGSLREIKDFEVMDNVYVCDASILKEPLGKPPIISIIALSKIFVRNYLMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPE9</accession>
    <name>N6VPE9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Large ribosomal subunit protein uL1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">rpl1P</name>
        <name evidence="11" type="synonym">rpl1</name>
        <name evidence="12" type="ORF">J422_06160</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Binds directly to 23S rRNA. Probably involved in E site tRNA release.</text>
    </comment>
    <comment type="function">
        <text evidence="10">Probably involved in E site tRNA release. Binds directly to 23S rRNA.</text>
    </comment>
    <comment type="function">
        <text evidence="11">Protein L1 is also a translational repressor protein, it controls the translation of its operon by binding to its mRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 11">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the universal ribosomal protein uL1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95760.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593263.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPE9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00551"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1200"/>
    <dbReference type="OrthoDB" id="10382at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006417">
        <property type="term" value="P:regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00403">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.790:FF:000005">
        <property type="entry name" value="50S ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01318_A">
        <property type="entry name" value="Ribosomal_uL1_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002143">
        <property type="entry name" value="Ribosomal_uL1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023674">
        <property type="entry name" value="Ribosomal_uL1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028364">
        <property type="entry name" value="Ribosomal_uL1/biogenesis"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016095">
        <property type="entry name" value="Ribosomal_uL1_3-a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023669">
        <property type="entry name" value="Ribosomal_uL1_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003244">
        <property type="entry name" value="PRK04203.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427">
        <property type="entry name" value="54S RIBOSOMAL PROTEIN L1, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36427:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL1M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00687">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002155">
        <property type="entry name" value="Ribosomal_L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56808">
        <property type="entry name" value="Ribosomal protein L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0678">Repressor</keyword>
    <keyword evidence="9 11" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="8 11 12" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="7 11" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 11" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="6 11" id="KW-0810">Translation regulation</keyword>
    <keyword evidence="4 11" id="KW-0820">tRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022491"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00045545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01318"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95760.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="221" mass="25034" checksum="1A38548F4ACBCB8F" modified="2013-06-26" version="1">MDREAILKAVKEARSLAKPRNFTQSFEFIATLKELDMRKPENRIKAEVVLPHGRGKEAKIAVIAEGPLAQQAEELGLTVIRKDQIEELGKNKRQLRKIAKAHDFFIAQADLMPLIGRYFGVILGPRGKMPKPVPANADIKPLVERLKKTVVINTRDKPYFQVLVGNEKMSDEQIVDNIEAVLNVVAKKYEKGLYHIKDAYVKLTMGPAVKIEKEKEKVKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPH0</accession>
    <name>N6VPH0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="9">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_05983</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95785.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593050.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPH0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00562"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1165"/>
    <dbReference type="OrthoDB" id="8615at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042026">
        <property type="term" value="P:protein refolding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861:SF3">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0143">Chaperone</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="10">
        <location>
            <begin position="5"/>
            <end position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95785.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="152" mass="17111" checksum="59747F5772BD2188" modified="2013-06-26" version="1">MIKKGDIVKIDYVLEVDGKVLDTSIEDVAREYGIYFEGKEYEPLEFIVGNNEVITGLEEAVLGMNVGEEKLVKIPPEKAYGFRDESLIQKVPKDLFKDADFEPEKGLLILANGIPAKIVDVDDEYVTLDFNHELAGKELTLKIIVKDVKSMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPH9</accession>
    <name>N6VPH9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transposase zinc-ribbon domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05918</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000046">
        <property type="protein sequence ID" value="ENN95795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592966.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPH9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00988"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024442">
        <property type="entry name" value="Transposase_Zn_ribbon"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12760">
        <property type="entry name" value="Zn_ribbon_IS1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transposase zinc-ribbon" evidence="1">
        <location>
            <begin position="17"/>
            <end position="66"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF12760"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="96" mass="11257" checksum="8918269D19348F27" modified="2013-06-26" version="1">MWVVFISAGIYKLFSPKDEERIEVIMRIRWDKGYICPFCGSRDVILKGKKKGRPHIKRYKCNSCGKNFNDLTGTIFAKKRMSLGEMFYIIKNLKNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ03</accession>
    <name>N6VQ03_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SirA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04990</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95970.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592111.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ03"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00491"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.978"/>
    <dbReference type="OrthoDB" id="45650at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00291">
        <property type="entry name" value="SirA_YedF_YeeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="entry name" value="TusA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279:SF18">
        <property type="entry name" value="SULFUR CARRIER PROTEIN MJ0990-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="1">
        <location>
            <begin position="4"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95970.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="71" mass="8104" checksum="1B22955FDBA9EE14" modified="2013-06-26" version="1">MKRLDVTGDICPIPVLKTKKALEELKEGEELEVIGDYKPALENIRRFAENNGYTVILAEETDNGFRIVIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ51</accession>
    <name>N6VQ51_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Preprotein translocase subunit SecG</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_04695</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Endomembrane system</location>
            <topology evidence="8">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the SEC61-beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96010.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591976.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ51"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00957"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.918"/>
    <dbReference type="OrthoDB" id="43651at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016482">
        <property type="entry name" value="SecG/Sec61-beta/Sbh"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002318">
        <property type="entry name" value="PRK01253.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03911">
        <property type="entry name" value="Sec61_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103460">
        <property type="entry name" value="Sec-beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0811">Translocation</keyword>
    <keyword evidence="3 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="33"/>
            <end position="52"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037847"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96010.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="12">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="53" mass="5948" checksum="6B06CFD3A2AB57D4" modified="2013-06-26" version="1">MSKREERGLATSAGLVRYMDESLSKIKVKPEHVIGLTVAFIIIETILTYGRLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQI7</accession>
    <name>N6VQI7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coenzyme F420 hydrogenase subunit alpha</fullName>
            <ecNumber evidence="2">1.12.98.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_03763</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.1" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96145.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQI7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00385"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050454">
        <property type="term" value="F:coenzyme F420 hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017682">
        <property type="entry name" value="Coenz_F420_hydrogenase_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03295">
        <property type="entry name" value="frhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43600:SF1">
        <property type="entry name" value="COENZYME F420 HYDROGENASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43600">
        <property type="entry name" value="COENZYME F420 HYDROGENASE, SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0533">Nickel</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="3">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="60"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="348"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="NCBIfam" id="TIGR03295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR601501-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96145.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="380" mass="43004" checksum="65934C9091337A0C" modified="2013-06-26" version="1">MRIELSPTTRHEGHSKLILEVEGEIVKRAYYINTTPVRGFESMLRGKPIEFAPIAVMRICGICQATHGIASCEAIENALNCEVPKDGLLLRELIGLGNRMHSHPLHHLLTLDDFIKEGEEELKIELIKIIQKMRKIGQMIVDIVGGEGIHPPNLVVGGVRNNITERAKAKIYYGLREYEKLAYELYEKYVPLVERFLEESGIPNLGMHNCPFLATSPTYGDREKIDWDQFIEIPPQRYYGNEIGLETSVMIPLYNGSPVEVGPRARMITFGKFKPKSALDINIARAKENFGAVYRAYEILDEVNINGKTRAEIEYKEGFGIGIHEAPRGTNVHMAETTKDGKIKRYSITAASTWNFPVVEKAIEGYPHQYAEVIMRAYDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQL6</accession>
    <name>N6VQL6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL32</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rpl32e</name>
        <name evidence="6" type="ORF">J422_03993</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96185.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591415.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQL6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00428"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.783"/>
    <dbReference type="OrthoDB" id="372100at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00513">
        <property type="entry name" value="Ribosomal_L32_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00810">
        <property type="entry name" value="Ribosomal_eL32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001515">
        <property type="entry name" value="Ribosomal_eL32"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023654">
        <property type="entry name" value="Ribosomal_eL32_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018263">
        <property type="entry name" value="Ribosomal_eL32_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036351">
        <property type="entry name" value="Ribosomal_eL32_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006332">
        <property type="entry name" value="PRK08562.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L32 AND DNA-DIRECTED RNA POLYMERASE II, SUBUNIT N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23413:SF1">
        <property type="entry name" value="RIBOSOMAL PROTEIN L32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01655">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01393">
        <property type="entry name" value="Ribosomal_L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52042">
        <property type="entry name" value="Ribosomal protein L32e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00580">
        <property type="entry name" value="RIBOSOMAL_L32E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96185.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="148" mass="17387" checksum="284A94636B91261C" modified="2013-06-26" version="1">MDRLLRLRFKLKRKKPDFIRTEAHRHKRLGEKWRRPRGRHNKMRLKWKEKPKVVSIGYRSPKAVRGLHPSGYEDVLVYNVKDLEKINPEKQAIRIASSVGMRKKIEIIKKAKELGIKILNISEEKQEELLKSLKGGNNEPNSTEEVSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQL9</accession>
    <name>N6VQL9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methanogenesis marker protein 15</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_03538</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96190.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591233.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQL9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01395"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.690"/>
    <dbReference type="OrthoDB" id="52668at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd24108">
        <property type="entry name" value="ASKHA_NBD_MJ0800-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002731">
        <property type="entry name" value="ATPase_BadF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008275">
        <property type="entry name" value="CoA_E_activase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051805">
        <property type="entry name" value="Dehydratase_Activator_Redct"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017676">
        <property type="entry name" value="Methan_mark_15"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00241">
        <property type="entry name" value="CoA_E_activ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03286">
        <property type="entry name" value="methan_mark_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32329:SF2">
        <property type="entry name" value="BIFUNCTIONAL PROTEIN [INCLUDES 2-HYDROXYACYL-COA DEHYDRATASE (N-TER) AND ITS ACTIVATOR DOMAIN (C_TERM)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32329">
        <property type="entry name" value="BIFUNCTIONAL PROTEIN [INCLUDES 2-HYDROXYACYL-COA DEHYDRATASE (N-TER) AND ITS ACTIVATOR DOMAIN (C_TERM)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01869">
        <property type="entry name" value="BcrAD_BadFG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase BadF/BadG/BcrA/BcrD type" evidence="5">
        <location>
            <begin position="148"/>
            <end position="401"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96190.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="412" mass="44436" checksum="84A3AC8FAFA46FA8" modified="2013-06-26" version="1">MVKIALLTCGAEWSGVYPEIEKAIKSVGGEVVFPEVELDYIEEVEEKLGFKVGSTNLKLLFARTMSIIEGKTEVDAAFVATCFRCAEGALVRNEVRKLIQENTNIPVVMYSFTERTKASELLTRMEALVTIVEKKALLARKKQEGVSLGIDSGSTTTKAVVMIDNEVVGTGWVYTRDVIGSAKEAVDNALKEAGISLDQVETIGTTGYGRFTVGEYYKADLIQEEITVNSKGAAYLADKQEGEATVIDIGGMDNKAISLYDAIPDSFTMGGICAGASGRFFEITARRLGVSIQELGELAAEGDWRKINMNSYCIVFGIQDLVTALAEGAKAEDVAAAACHSVAEQVYEQQLQEVDVREPVILVGGSSLLKGLVIAMEDILGKKIIVPKYSQFIGAVGAALLSSGYRYKKIKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQN3</accession>
    <name>N6VQN3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Putative heavy-metal chelation domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03623</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96205.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591260.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQN3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01379"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.707"/>
    <dbReference type="OrthoDB" id="147804at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11590">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007161">
        <property type="entry name" value="DUF364"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04016">
        <property type="entry name" value="DUF364"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159713">
        <property type="entry name" value="Dhaf3308-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Putative heavy-metal chelation" evidence="1">
        <location>
            <begin position="130"/>
            <end position="224"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96205.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="241" mass="27453" checksum="2A1E74256BE17CC5" modified="2013-06-26" version="1">MDYQELIDIIKKELKKLLEENELMDEEIKITPANVKLDSSRIKDYPLLNRREVLLRAYFKNAIGEAFTADIREFNGCVKDIFLLNHNPSLIATLNAVMRYLNLIDKTEHCQNSEPEECAKKLCEYLIDNYGEDIKVGIIGYQPAMIKCLAETFKNVIATDLNFDNVGRIRYGVPIYHGDMNEYLIKNSDVVLATGSTAVNGTICEILKLVRNYNKDIIFYGTSIAGVAKILNLKRFCLLGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQW8</accession>
    <name>N6VQW8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF169 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03009</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96295.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590746.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQW8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00149"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.590"/>
    <dbReference type="OrthoDB" id="89232at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003748">
        <property type="entry name" value="DUF169"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37954">
        <property type="entry name" value="BLL4979 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37954:SF3">
        <property type="entry name" value="DUF169 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02596">
        <property type="entry name" value="DUF169"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96295.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="230" mass="25675" checksum="329D1F3C577189D8" modified="2013-06-26" version="1">MEIEKIREYAKKIYELLGEDKPFVGVKLAKSKEEIPEGYSEIESEKRHCEMVQLARLERKKFYATLNKHLCKGGAYAIGLLKNPPEPLATGKLYVKLGNFKDENAARKTVDAIPKVKDEVYATVYAPLDDIDFIPDSIIAIVKPKKALRLVQASLYRDGGRFSADFSGIQSVCADAVAAVKVRGVSNMTLGCNGSRKYAGIKDEELVFAFPVDELEKIVEALEHFKEVWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR30</accession>
    <name>N6VR30_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_02764</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cell membrane</location>
            <topology evidence="1 10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the binding-protein-dependent transport system permease family. OppBC subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96365.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590611.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR30"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00104"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.542"/>
    <dbReference type="OrthoDB" id="44105at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015099">
        <property type="term" value="F:nickel cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045621">
        <property type="entry name" value="BPD_transp_1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050045">
        <property type="entry name" value="Opp2B"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045470">
        <property type="entry name" value="Opp2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43163:SF6">
        <property type="entry name" value="DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19300">
        <property type="entry name" value="BPD_transp_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0533">Nickel</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="105"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="138"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="165"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="235"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="277"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="11">
        <location>
            <begin position="99"/>
            <end position="300"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00024202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU363032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96365.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="311" mass="35346" checksum="55FA9661667D235B" modified="2013-06-26" version="1">MIKYILKRISYAFPVLIGISLISFSLLYVFPGDPAELIVSITSGTEPSKEMIEKFRKESGLDKPIYIQYINWLSHAIRGDFGKSWKSGEPVMDEIIKRLKPSLELFSLTFIISLEFAFILGTLSAIYKDKFIDNISRLFTLLGISIPSFWLGLILIWIFSVKLRILPAFGYGSIKHLILPVITWSFSFMAIKTRFIRSSILEVLNEDYILTARAKGLPERVILLKHALRNALIPIITYLGLSFHHLIIGSVIVESVFSWPGLGRYLVESVISRDFPVVQALVFLSGILMITVNLIIDILYAIIDPRIRYEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR35</accession>
    <name>N6VR35_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">DEAD/DEAH box helicase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_07057</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000070">
        <property type="protein sequence ID" value="ENN95581.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594796.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000070.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR35"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00852"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1366"/>
    <dbReference type="OrthoDB" id="39583at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18795">
        <property type="entry name" value="SF2_C_Ski2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3380.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043852">
        <property type="entry name" value="DUF5814"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050474">
        <property type="entry name" value="Hel308_SKI2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961:SF1">
        <property type="entry name" value="ATP-DEPENDENT HELICASE MJ1401-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47961">
        <property type="entry name" value="DNA POLYMERASE THETA, PUTATIVE (AFU_ORTHOLOGUE AFUA_1G05260)-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19131">
        <property type="entry name" value="DUF5814"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="71"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="242"/>
            <end position="417"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95581.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="656" mass="75329" checksum="6AE690B57E0CAE30" modified="2013-06-26" version="1" fragment="single">KKFKDVDKVLSLLRIKNPLNHPELTRYDIIESSEEDIECYKIDDLNIPEKLKKILKERYNTLLPVQTLAIKAGLLEGKDLLIVSATSSGKTLIGEIAGIKNLLEGKGKMIYLTPLIALANQKYFEFKEKYNFKVSLRVGFGRIGKKLDVDRDINADIIVGTYEGIDYLIRTNKLKDVGTVVVDEIHSLNMPERGARLDGLIGRLRYLFNAQMIYLSATIGNPKELAKRLNANLVLYNKRPVPLERHIIFCKNEFHKLSIIREIVKREWNNISKYGYRGQSLIFTFSRKRAEYIARSLKAKGIKAEFYHGGMDYFRRRRVEEDFLNQKIQCVVTTAALSAGVDFPASTVILESLAMGGDWLSPSDFQQMCGRAGRKGMHELGKVYLLVEIGKKYHAKMQETEDEVAVKLLNAEPEKIDVEYSEDEEEEQILATVSAGVNNIYDIDRVPYLGRSHHLNAILNNLKSYGMINLEGKEVKLTKYGRAVAISFLYPKVAEKIKEAIKKEKELIKLIKEIYPFENVYLSQNLQKSLSKILKINVPSRFFDALEVIKEALKKVEDKKLKDILTMIIIEFEHNPEEKIIEELINLRSLGKTPGQIAKYLYENYKILTYAGDIHYYLEQLLNILDAVERIALIYNKRYANKVKELKDKIENKSVS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR50</accession>
    <name>N6VR50_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02894</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96385.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590651.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR50"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00127"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.568"/>
    <dbReference type="OrthoDB" id="61889at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96385.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="263" mass="31344" checksum="4FD9EE61D5FF4C51" modified="2013-06-26" version="1">MKAKELAKKLLFDIYKNLDEFSKDIIRCDLADIEFKGFYLKGKNGEKVYIRTLEDFENLEDFEVEERKYKLKNINLKHFEDGLMIINLSSKKSKNYKFEADYTITYPSYDVTAEFRERMIKWKEMDEEEMDKAIAEFDNKVNDILSDILDEVKIGKRVSAHLDVFVDSHKLENFVDEGEDIIIIWIHPAFLYSDDKILKGLLAYELSKFNKKFLEKYYKDILLYCKEIKNLTNKTPKIIEKIRNIALKYNDTLTLNLINEMEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR67</accession>
    <name>N6VR67_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11">Probable cobalamin biosynthesis protein CobD</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">cobD</name>
        <name evidence="12" type="ORF">J422_02454</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 11">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 11">Cell membrane</location>
            <topology evidence="2 11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 11">Belongs to the CobD/CbiB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96400.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590431.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR67"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00782"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.480"/>
    <dbReference type="OrthoDB" id="46105at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048472">
        <property type="term" value="F:threonine-phosphate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00024">
        <property type="entry name" value="CobD_CbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004485">
        <property type="entry name" value="Cobalamin_biosynth_CobD/CbiB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00380">
        <property type="entry name" value="cobal_cbiB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002281">
        <property type="entry name" value="PRK01209.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308">
        <property type="entry name" value="COBALAMIN BIOSYNTHESIS PROTEIN CBIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34308:SF1">
        <property type="entry name" value="COBALAMIN BIOSYNTHESIS PROTEIN CBIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03186">
        <property type="entry name" value="CobD_Cbib"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 11" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="9 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="53"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="158"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="288"/>
            <end position="307"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006263"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00016185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96400.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="311" mass="35200" checksum="33B38102480B231E" modified="2013-06-26" version="1">MINPIILYITIIVDVIFGEPNEKVHPTVLIGRLISLLERLLPSTYSNNKIRDFLFGSVVTVTTILIVGYISYIVEKLILALPKHLSYLLYAILLSTTIGYRSLKEFCIKPIEALIEGDLEKARELVKHVVSRNTEDLDKEHILSASIESLSENLTDSVISPLFYAIFFGLVGAFIYRTVNTLDAMIGYKNSKYYYYGKLAARLDDILNFIPSKISGLLLIITAPFYGGDWKRALYGFLFESLKTPSPNSGFTMATLANALNMSLEKIGYYKLGNGRIDIDKSLRAFKAIDYSIIAFLLIYTLLWWLFEGFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR70</accession>
    <name>N6VR70_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Ser/Thr phosphatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06797</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000062">
        <property type="protein sequence ID" value="ENN95631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594210.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR70"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01092"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1319"/>
    <dbReference type="OrthoDB" id="198002at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004722">
        <property type="term" value="F:protein serine/threonine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00143">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.40.10">
        <property type="entry name" value="PPM-type phosphatase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015655">
        <property type="entry name" value="PP2C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036457">
        <property type="entry name" value="PPM-type-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001932">
        <property type="entry name" value="PPM-type_phosphatase-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47992">
        <property type="entry name" value="PROTEIN PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13672">
        <property type="entry name" value="PP2C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00331">
        <property type="entry name" value="PP2C_SIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00332">
        <property type="entry name" value="PP2Cc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81606">
        <property type="entry name" value="PP2C-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51746">
        <property type="entry name" value="PPM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PPM-type phosphatase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="237" mass="27004" checksum="2F3DD52A168AB050" modified="2013-06-26" version="1">MKVFGITHRGGRDKNEDHILIKKIGDIYLLAVADGLGGHNAGDIASKIAIEELEKFFEKNCYKSLLYEEIETILKEAFKKVHNAILNQSYGDREEMGTTLTSVVIKDNKAIIANCGDSRAYIIRDGKIIFKTKDHTYVQELIDKGYLSEEEAMYHPYKHVLKHALGIDFAVDIYKKELKSGDILLLSSDGLHDYVREKEILEVINKYEEPKDIVENLLKIALKKTMDNVSIIVFKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR73</accession>
    <name>N6VR73_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Major facilitator superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_02479</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96405.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
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        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR73"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00777"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.485"/>
    <dbReference type="OrthoDB" id="117970at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17325">
        <property type="entry name" value="MFS_MdtG_SLC18_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1250.20">
        <property type="entry name" value="MFS general substrate transporter like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011701">
        <property type="entry name" value="MFS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020846">
        <property type="entry name" value="MFS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052714">
        <property type="entry name" value="MFS_Exporter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036259">
        <property type="entry name" value="MFS_trans_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001958">
        <property type="entry name" value="Tet-R_TetA/multi-R_MdtG-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531:SF2">
        <property type="entry name" value="PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23531">
        <property type="entry name" value="QUINOLENE RESISTANCE PROTEIN NORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07690">
        <property type="entry name" value="MFS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01035">
        <property type="entry name" value="TCRTETA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103473">
        <property type="entry name" value="MFS general substrate transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50850">
        <property type="entry name" value="MFS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="30"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="42"/>
            <end position="62"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="69"/>
            <end position="88"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="128"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="159"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="229"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="261"/>
            <end position="280"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="286"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="324"/>
            <end position="345"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="351"/>
            <end position="371"/>
        </location>
    </feature>
    <feature type="domain" description="Major facilitator superfamily (MFS) profile" evidence="6">
        <location>
            <begin position="4"/>
            <end position="375"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96405.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="375" mass="40760" checksum="01BBEF2991933D00" modified="2013-06-26" version="1">MDKNLFIIWVTTFVTMLGVGFIAPIIPIYAQTLGATNFEIGLIFGSFALARTLTQVPVGILSDIYGKKFFIVSGTFFYGLFTLLYNFVNTVFDLLIVRALTGAFSSFVTPVAGSYVAAIAPKDRLGEYMGIFNSAITLGFGVGPFIGGTLADIYGIKTPFYFCGFLGILAAIISYTKLEDIKFKKENCDKISLEFLKNKNFLYSFIINTSNVMMNAGIITYLGIYTIKYGISIAQIGSMIAGTNLLMAALQRSFGKLYDKLGKVVIIFGICIASCGVYFLSLSSSFWKIMFSLATVAIGISMCSPATTSIAVKDIPPYRKGGAMGIFTTSINIGMFLGSVFFGFLADIFGISGMFKASAMFSIIVGFICYLKIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR95</accession>
    <name>N6VR95_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF112 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06563</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000055">
        <property type="protein sequence ID" value="ENN95671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593716.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR95"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00606"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1277"/>
    <dbReference type="OrthoDB" id="53365at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002823">
        <property type="entry name" value="DUF112_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42204">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42204:SF1">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01970">
        <property type="entry name" value="TctA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="119"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="145"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="171"/>
            <end position="190"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="317"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
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            <end position="342"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="349"/>
            <end position="367"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="373"/>
            <end position="390"/>
        </location>
    </feature>
    <feature type="domain" description="DUF112" evidence="2">
        <location>
            <begin position="2"/>
            <end position="377"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="391" mass="43708" checksum="6CA52CF2F9042DE9" modified="2013-06-26" version="1">MIYLILGIICGIFTGLFPGIHPNNIAALSFLLYPYFGEHFINFLIGLVVTHYFINFIPAAFLGIPDDETAVSVLPMHRLSIIGEGFKAILLSGIGSYLGVLFSLLISFLIIFLKLNINYIFSNIKPFIPYILILFVIYQIISAKNVWEVLVILLSGLFGIAVLYYHTSYNITLTAIFTGMYGIPILLNNLNSKFKKQRITYPNFSLKYIKITFFASLIGFFRIFLPAIGGAQLNYFLSKIIRENEIESFLVSQGAIVLSNEIFSLLALVFIGTGRSGVANTIKELNLNFSLDQLSLSIILSATLAFIILIYLSKFILSILERVNIRVLSILLIFICTLIIIVGSYNFGVVYHLIVYFSSVFLGLLAVRSGSNLTNLMNVLVFPTILIYLGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRE7</accession>
    <name>N6VRE7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Radical SAM core domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_02210</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96465.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590269.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRE7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01190"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.434"/>
    <dbReference type="OrthoDB" id="63821at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017672">
        <property type="entry name" value="MA_4551-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03278">
        <property type="entry name" value="methan_mark_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF35">
        <property type="entry name" value="MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="1"/>
            <end position="250"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96465.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="407" mass="46193" checksum="D4528C70ADC20B6C" modified="2013-06-26" version="1">MNLLVDLKGEPGKNCNGFCKFCYFRKINKNNIEPLGCKYCQFTIGCDYCLYSVREMNGDFLPLPFVISQIQSGLLFKRYEKVNITAGGDVSCYPYLEELCKWINKLGLNIHLGYTSGKGFYDKEIAKRLVDYGVDEVTFSVFSTNPQLRKEWMNDKNAETALKCLEYFCEHCEVHCAILVIPGINDKDLEKTVSDLVNFGAKAVILMRFANREDQGLILGNAPIIEGVEPHSLEEFKNIVKQVHDEFGDKIRVTGTPLYDPIAGTPFAIASEDKILERLRNKVEGEATIITGRVAYPYLRKIFNDTDVNIVKVEKDIADLITAKDLEKINLKELKDTVIFPGKAFVHDEVAKKLLSRDRERIVVRGVDQLTLDGEVSGSYTREEAIKFEIEAFNELIGIINFFGMKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRH1</accession>
    <name>N6VRH1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methyl-accepting chemotaxis sensory transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02320</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96485.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRH1"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.456"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96485.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="43" mass="5025" checksum="BC88CF13F1CC6916" modified="2013-06-26" version="1" fragment="single">TASTEELNSTISEFRKIIIDLKETSKIAEELSLRFKINNEFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRH4</accession>
    <name>N6VRH4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">PfkB domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_06165</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the carbohydrate kinase PfkB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593265.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRH4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00552"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1201"/>
    <dbReference type="OrthoDB" id="26949at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006796">
        <property type="term" value="P:phosphate-containing compound metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01942">
        <property type="entry name" value="ribokinase_group_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002173">
        <property type="entry name" value="Carboh/pur_kinase_PfkB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011611">
        <property type="entry name" value="PfkB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002139">
        <property type="entry name" value="Ribo/fructo_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584:SF166">
        <property type="entry name" value="RIBOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10584">
        <property type="entry name" value="SUGAR KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00294">
        <property type="entry name" value="PfkB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00990">
        <property type="entry name" value="RIBOKINASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00583">
        <property type="entry name" value="PFKB_KINASES_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00584">
        <property type="entry name" value="PFKB_KINASES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Carbohydrate kinase PfkB" evidence="5">
        <location>
            <begin position="2"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010688"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="298" mass="33664" checksum="271E501B63C8CF00" modified="2013-06-26" version="1">MITCIGHIALDYIFNIDRFPEPNSSVQIPSAKLYYGGAAANTAVAIKKLGVDCELISCVGYDFKDSRYEKYLKKLGVNISKLYYSEDEETPKAWIFTDKENNQITFFLWGAAKHYKELNPPNFDTNIVHIATGDPDYNLRAAKKAYGKAIVSFDPGQDLPQYSENSLRELIKHTNFLFLNKYEFDRISKMLNFNLDDFLKYVDILVVTKGEKGSTIYTEENKKIEIPVIKAKVVDPTGAGDAYRAGFLVAYLKKYPLEKCGLIASAAASFVVEAKGSQTNLPTWDMVLNRLKRHGFNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRU5</accession>
    <name>N6VRU5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Magnesium chelatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_05439</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunits D/I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000039">
        <property type="protein sequence ID" value="ENN95886.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592482.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRU5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00837"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1062"/>
    <dbReference type="OrthoDB" id="25914at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.80">
        <property type="entry name" value="Magnesium chelatase subunit I, C-Terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045006">
        <property type="entry name" value="CHLI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041628">
        <property type="entry name" value="ChlI/MoxR_AAA_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000523">
        <property type="entry name" value="Mg_chelatse_chII-like_cat_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32039:SF9">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLI-2, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17863">
        <property type="entry name" value="AAA_lid_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01078">
        <property type="entry name" value="Mg_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="27"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="225"/>
            <end position="252"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95886.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="341" mass="38724" checksum="E2F44FE046CED282" modified="2013-06-26" version="1">MKVYPFTAIVGQEKMKLALILNAINPKIGGVLIRGEKGTAKSTAVRALADLLPEIEVIEGCPFNCDPKEPCDLCKERDNIRVIKRKMKVVNLPIGATEDRVVGTLDIEKAIKEGIKALEPGILAEANRNILYIDEVNLLDDHIIDLLLDAAASGWNIIEREGIKIKHPSRFILVGTMNPEEGELRPQILDRFGLMVEVEGLKDPKQRVEVVKRVEEFSKDPEGFYKKFEKEQEKLRERILKARKILDKVEIDEDLLELISKTCVELGIFTNRADITVVRTAKAIAAYDNREKVNLEDVKLAMELALPHRMRRKPFEPPRIDKNRLEEIINKNLDGLKKNIT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRZ4</accession>
    <name>N6VRZ4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit Rpo5</fullName>
            <ecNumber evidence="5">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">DNA-directed RNA polymerase subunit H</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">rpoH</name>
        <name evidence="5" type="synonym">rpo5</name>
        <name evidence="7" type="ORF">J422_05174</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5">Belongs to the archaeal Rpo5/eukaryotic RPB5 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000033">
        <property type="protein sequence ID" value="ENN95931.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592244.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRZ4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01222"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1012"/>
    <dbReference type="OrthoDB" id="30537at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006362">
        <property type="term" value="P:transcription elongation by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.940.20:FF:000001">
        <property type="entry name" value="DNA-directed RNA polymerases I, II, and III subunit RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.20">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00025">
        <property type="entry name" value="RNApol_Rpo5_RPB5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014381">
        <property type="entry name" value="Arch_Rpo5/euc_Rpb5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000783">
        <property type="entry name" value="RNA_pol_subH/Rpb5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020608">
        <property type="entry name" value="RNA_pol_subH/Rpb5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035913">
        <property type="entry name" value="RPB5-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007129">
        <property type="entry name" value="PRK09570.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10535:SF0">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01191">
        <property type="entry name" value="RNA_pol_Rpb5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55287">
        <property type="entry name" value="RPB5-like RNA polymerase subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01110">
        <property type="entry name" value="RNA_POL_H_23KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 5 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="2 5 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0804">Transcription</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase subunit H/Rpb5 C-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="73"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01191"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95931.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="78" mass="9025" checksum="AB2794FE4DABF7CF" modified="2013-06-26" version="1">MKVTDHILVPKHEIVPKEEVEKILKMYNIKLQQLPKIYEDDPVIQEIGAKEGDVIKITRKSPTAGTFVVYRLVIKRIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS17</accession>
    <name>N6VS17_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">3-keto-alpha-glucoside-1,2-lyase/3-keto-2-hydroxy-glucal hydratase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04895</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95951.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592073.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS17"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00512"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.959"/>
    <dbReference type="OrthoDB" id="64717at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.560">
        <property type="entry name" value="Exo-inulinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010496">
        <property type="entry name" value="AL/BT2_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06439">
        <property type="entry name" value="3keto-disac_hyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-keto-alpha-glucoside-1,2-lyase/3-keto-2-hydroxy-glucal hydratase" evidence="1">
        <location>
            <begin position="44"/>
            <end position="200"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95951.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="205" mass="23427" checksum="E35028768E3EECD9" modified="2013-06-26" version="1">MRRFIVILIIALMSGCINIQFMPPPLNDDFSSYNLGEKAPFGPWKVKGNGTFTITPIFSEDNKVMNKVVEAEGDGIMYVDRNYTNFVYYIDIKRKKLIDSPKIYFRLNGDATAGYCIVVEPFDRGYKLYKFNGTKWVLLNQTYNAAPAEVSFFRYMVIANGNKITFKVNAYTYITYIDKNPILEGGIGFGGQGYFDNVRVEPLEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS71</accession>
    <name>N6VS71_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NADH ubiquinone oxidoreductase 20 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00766</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589825.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS71"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00883"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.149"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.700">
        <property type="entry name" value="NADH:ubiquinone oxidoreductase-like, 20kDa subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051349">
        <property type="entry name" value="Hydrogenase_assoc-protein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037024">
        <property type="entry name" value="NiFe_Hase_small_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045875">
        <property type="entry name" value="F420_non_VhuG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42845">
        <property type="entry name" value="COENZYME F420-REDUCING HYDROGENASE, GAMMA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42845:SF2">
        <property type="entry name" value="F420-NON-REDUCING HYDROGENASE VHU SUBUNIT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="2">
        <location>
            <begin position="12"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="283" mass="31012" checksum="C4D50D19E738B559" modified="2013-06-26" version="1">MAKIGMIQLCGCSGCHISLLDLHDQLLDLIPNIEIVYAPIIADPKEIPDGIDVFLVEGGVRNEHDEHLLEEIREKSKIVVAFGTCAAYGGIPGLGNLYTKEDLLNTVYSTDTTENKEIPNEEIPELTEVVKPISHVIKVDYILPGCPPCPNIIKDALLAILNGEEPKIPQKIVCDECPRKKENVYPKEFKRPFEGKPDPERCLFEQGYPCLGFATRAGCGAKCPKSGMPCRGCYGKTDAVLDFGAKAANVLANSGEHALKLPDKLGLLYRFCLPAALINRKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS78</accession>
    <name>N6VS78_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">N5-carboxyaminoimidazole ribonucleotide mutase</fullName>
            <shortName evidence="3 4">N5-CAIR mutase</shortName>
            <ecNumber evidence="3 4">5.4.99.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5-(carboxyamino)imidazole ribonucleotide mutase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purE</name>
        <name evidence="7" type="ORF">J422_04675</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 4">Catalyzes the conversion of N5-carboxyaminoimidazole ribonucleotide (N5-CAIR) to 4-carboxy-5-aminoimidazole ribonucleotide (CAIR).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3 4">
            <text>5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole + H(+) = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate</text>
            <dbReference type="Rhea" id="RHEA:13193"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58730"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="EC" id="5.4.99.18"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 4">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (N5-CAIR route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the AIR carboxylase family. Class I subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.18" evidence="3 4"/>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96006.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591961.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS78"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00953"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.914"/>
    <dbReference type="OrthoDB" id="9473at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00943"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034023">
        <property type="term" value="F:5-(carboxyamino)imidazole ribonucleotide mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01929">
        <property type="entry name" value="PurE_classI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033747">
        <property type="entry name" value="PurE_ClassI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000031">
        <property type="entry name" value="PurE_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024694">
        <property type="entry name" value="PurE_prokaryotes"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01162">
        <property type="entry name" value="purE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046:SF2">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23046">
        <property type="entry name" value="PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00731">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001338">
        <property type="entry name" value="AIR_carboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01001">
        <property type="entry name" value="AIRC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52255">
        <property type="entry name" value="N5-CAIR mutase (phosphoribosylaminoimidazole carboxylase, PurE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 3 4" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurE" evidence="6">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3 5">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01929"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR001338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR001338-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96006.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="157" mass="17252" checksum="5DD4A6A5BF951226" modified="2013-06-26" version="1">MICIIMGSESDLKIAEKAINVLKEFNVKYEVRVASAHRTPELIEEIVKNPEIEVFIAIAGLAAHLPGVIASLTLKPVIAVPVDVKLCGLDALLSSVQMPPGVPVATVGVDRGENAAILALQILALKDKELYNKLLEYRNKMKEKVYNSDEKIKKMFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS85</accession>
    <name>N6VS85_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="16">Branched-chain-amino-acid aminotransferase</fullName>
            <shortName evidence="16">BCAT</shortName>
            <ecNumber evidence="16">2.6.1.42</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ilvE</name>
        <name evidence="17" type="ORF">J422_00851</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 16">Acts on leucine, isoleucine and valine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 16">
            <text>L-isoleucine + 2-oxoglutarate = (S)-3-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24801"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:35146"/>
            <dbReference type="ChEBI" id="CHEBI:58045"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14 16">
            <text>L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18321"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:17865"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57427"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 16">
            <text>L-valine + 2-oxoglutarate = 3-methyl-2-oxobutanoate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:24813"/>
            <dbReference type="ChEBI" id="CHEBI:11851"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="EC" id="2.6.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 16">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 16">Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5 16">Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 4/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="4 16">Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 4/4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 15">Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.42" evidence="16"/>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589840.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS85"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00864"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.168"/>
    <dbReference type="OrthoDB" id="6469at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00058"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00048">
        <property type="reaction ID" value="UER00073"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00049">
        <property type="reaction ID" value="UER00062"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052656">
        <property type="term" value="F:L-isoleucine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052654">
        <property type="term" value="F:L-leucine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052655">
        <property type="term" value="F:L-valine-2-oxoglutarate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009099">
        <property type="term" value="P:L-valine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd01558">
        <property type="entry name" value="D-AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.10:FF:000006">
        <property type="entry name" value="Branched-chain-amino-acid aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.10.10:FF:000002">
        <property type="entry name" value="D-alanine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.10.10">
        <property type="entry name" value="D-amino Acid Aminotransferase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001544">
        <property type="entry name" value="Aminotrans_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018300">
        <property type="entry name" value="Aminotrans_IV_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036038">
        <property type="entry name" value="Aminotransferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005785">
        <property type="entry name" value="B_amino_transI"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043132">
        <property type="entry name" value="BCAT-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043131">
        <property type="entry name" value="BCAT-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050571">
        <property type="entry name" value="Class-IV_PLP-Dep_Aminotrnsfr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01122">
        <property type="entry name" value="ilvE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005146">
        <property type="entry name" value="PRK06606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006185">
        <property type="entry name" value="PRK08320.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743">
        <property type="entry name" value="AMINO-ACID AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42743:SF11">
        <property type="entry name" value="AMINODEOXYCHORISMATE LYASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01063">
        <property type="entry name" value="Aminotran_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56752">
        <property type="entry name" value="D-aminoacid aminotransferase-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00770">
        <property type="entry name" value="AA_TRANSFER_CLASS_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 16" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 16 17" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="11 16" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="10 16" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 16 17" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00005072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048212"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004106"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="RuleBase" id="RU364094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN96740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="285" mass="31955" checksum="A04B8D46B441A48E" modified="2013-06-26" version="1">MKIYLNGKFVDKEEAKISVFDHGLLYGDGVFEGIRAYDGVVFMLKEHIDRLYDSAKAICLDIPLTKEEMIEVVLNTLRVNGLKDAYIRLVITRGEGDLGLDPRKCKNPNIICIAVPMDPLLGEDGIRVITASVRRLPVDVLPPAVKSLNYLNSILAKLQANYANVDEAFLLDENGFIVEGTGDNIFVVKNNILKTPPVYYSVLKGITRDVVIKLAKEEGIEVREEPLTLYDLYTADEVFITGTAAEIVPVFEIDGRKINNGKIGEITKRLKEKFKEVRKKWGIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSP9</accession>
    <name>N6VSP9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_03698</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96216.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591282.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSP9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01366"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.722"/>
    <dbReference type="OrthoDB" id="114744at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031125">
        <property type="term" value="P:rRNA 3'-end processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050798">
        <property type="entry name" value="YhaM_exoribonuc/phosphodiest"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37294">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE YHAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37294:SF1">
        <property type="entry name" value="3'-5' EXORIBONUCLEASE YHAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="2">
        <location>
            <begin position="59"/>
            <end position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96216.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="233" mass="26831" checksum="84C5A3A0CD11638E" modified="2013-06-26" version="1">MVERLIELANLIKDEELRKKVIDFIMNPKATHPEIKDTGVSLEESPASINWHHKYEGGLIEHTVSVTKLAIKIAEVLEEVYKVEINKDYVIAGALLHDIMKPYNYIKTDEGFDHYDLFNLDHLTLAVAELYKRNFPLKVIKIVASHHGEFSPTKPSSIEEYIVHYADELDAKINDAAIRVCSARSRDLGIDESEIYKVITPLKVYEIRSKEGREKCIKFIKELLSLDFNFDDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSS1</accession>
    <name>N6VSS1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphate transport system permease protein PstA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_03248</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cell membrane</location>
            <topology evidence="1 9">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the binding-protein-dependent transport system permease family. CysTW subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96246.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590942.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSS1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01037"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.636"/>
    <dbReference type="OrthoDB" id="11402at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005315">
        <property type="term" value="F:phosphate transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06261">
        <property type="entry name" value="TM_PBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3720.10">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000515">
        <property type="entry name" value="MetI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035906">
        <property type="entry name" value="MetI-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005672">
        <property type="entry name" value="Phosphate_PstA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051408">
        <property type="entry name" value="Phosphate_transprt_permease"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00974">
        <property type="entry name" value="3a0107s02c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42922">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42922:SF1">
        <property type="entry name" value="PHOSPHATE TRANSPORT SYSTEM PERMEASE PROTEIN PSTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00528">
        <property type="entry name" value="BPD_transp_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF161098">
        <property type="entry name" value="MetI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50928">
        <property type="entry name" value="ABC_TM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="12"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="61"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="104"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="132"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="191"/>
            <end position="217"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="251"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transmembrane type-1" evidence="10">
        <location>
            <begin position="65"/>
            <end position="270"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363043"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96246.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="280" mass="30458" checksum="633B2AF8757BA2FA" modified="2013-06-26" version="1">MSHKRIRMIKDKLFLIVIGCLTFLAVLPLFHIIFSIILKGLPVVLEGGLKFLTATKSEGGIGPAIIGTLYLTFLASLIGLPLAFLAGTYAYEFPKSFIGKATRVLLQIMLEFPTILVGTFIMAVVVVPLGSFSAIAGALALALIMTPYVAVYTEEAMSEIPRIYKEGAYALGCTRAQVIFKVITKMAKKGILTGMLIGMAKVAGETAPLIFTIGGLYESYPDLLNPLKPIGAIPMLIYELINSPSPEDHKMAWGTALIMLIIFLSIFIPIRYALREDIKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSU6</accession>
    <name>N6VSU6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_03196</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000019">
        <property type="protein sequence ID" value="ENN96276.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590891.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSU6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00316"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.627"/>
    <dbReference type="OrthoDB" id="2837at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000813">
        <property type="entry name" value="7Fe_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050954">
        <property type="entry name" value="ET_IronSulfur_Cluster-Binding"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177">
        <property type="entry name" value="PROTEIN NRFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43177:SF3">
        <property type="entry name" value="PROTEIN NRFC HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13247">
        <property type="entry name" value="Fer4_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00354">
        <property type="entry name" value="7FE8SFRDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="4"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="64"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96276.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="146" mass="16449" checksum="D14F5DA40909E45A" modified="2013-06-26" version="1">MNPKIIVINPEKCSKCNDCVNVCREIHGISRVRKYNNVPMFCMQCEKAPCKEVCPVGAIYLKENIPIVNKEKCIGCGMCVIACPIGAIFIEDKVAHKCTLCLDTDRVSPACVEACKDRALILVTDETLELYKEERRKKILDVLKQE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT29</accession>
    <name>N6VT29_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Chromosome assembly protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02684</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96351.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590581.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT29"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00089"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.526"/>
    <dbReference type="OrthoDB" id="66058at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96351.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="106" mass="12238" checksum="44D2C0D267B2E8DF" modified="2013-06-26" version="1">MSEKDVKEIKIEVPSELIEKIKKVSEFLTSLKEKKVELSKEKIEEMIKIIDEIKNKLPEIKIDIEKILDIIEGSGSEVKLVFEDLTFSIDGEVGLKIIPLKREKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT60</accession>
    <name>N6VT60_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">NYN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02864</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96381.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590642.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT60"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00122"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.562"/>
    <dbReference type="OrthoDB" id="85234at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004540">
        <property type="term" value="F:RNA nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18726">
        <property type="entry name" value="PIN_LabA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002790">
        <property type="entry name" value="CHP00288"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021139">
        <property type="entry name" value="NYN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00288">
        <property type="entry name" value="TIGR00288 family NYN domain-containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35811:SF1">
        <property type="entry name" value="HTH OST-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35811">
        <property type="entry name" value="SLR1870 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01936">
        <property type="entry name" value="NYN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NYN" evidence="1">
        <location>
            <begin position="24"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96381.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="174" mass="19898" checksum="D8670ED63B0852B5" modified="2013-06-26" version="1">MWKKLESLTNKLYEKARRKRGHHKIALLIDGPNMLRKEFNIDLDKIRELLSEFGDIIIGRVYLNQYASDKLIEAVINQGFEPKISAGDVDVEMAVDATELVFNPNIDTIAYVTRDADFLPAIRKAKERGKKVIVIGAEPGFSTALQNIADIVIKINEEFQLDKEKIKKKKEEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTH2</accession>
    <name>N6VTH2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02290</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96481.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTH2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01206"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.450"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.400.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016437">
        <property type="entry name" value="MCT-1/Tma20"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798:SF0">
        <property type="entry name" value="MALIGNANT T-CELL-AMPLIFIED SEQUENCE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22798">
        <property type="entry name" value="MCT-1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PUA" evidence="1">
        <location>
            <begin position="1"/>
            <end position="54"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96481.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="64" mass="7208" checksum="5E6A688AC786EA69" modified="2013-06-26" version="1">MVPGIKEVDNFNKGDVVFVLDEVHKKPICVGIALMSHEEIKNSEKGKAIKNIHYVGDKIWNFKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTI5</accession>
    <name>N6VTI5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">AIR synthase related protein domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01690</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96491.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590096.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTI5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00189"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.332"/>
    <dbReference type="OrthoDB" id="42306at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02691">
        <property type="entry name" value="PurM-like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009186">
        <property type="entry name" value="Ni_metllenz_mat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270:SF2">
        <property type="entry name" value="HYDROGENASE EXPRESSION_FORMATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006346">
        <property type="entry name" value="Ni_metllenz_mat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="1">
        <location>
            <begin position="114"/>
            <end position="228"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="2">
        <location>
            <begin position="240"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96491.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="447" mass="49678" checksum="F71FB02203A67731" modified="2013-06-26" version="1">MDIEGYVRRCLRKNIPEEKIIEDGFKRIVEIKEDEEFAKKLIKAVLEEVKISEKALNVEDKNLKIILNYPKANVKMGEFGVGSRGEGDFFVHREIARIIESTNTVSYTSAKDQDDAGIVKVDKAKYIVAAVDGTHSRLSEFPFLAGFHVARAALRDVYVKGARGIALLSDIHLADDGDIGKIFDFTAGVCCVSEALNIPLIGGSTLRVGGDMVIGDRMVSSVTAIGVIDDYEPTVRKRAKEGDVILMTKGSGGGTITTTAIFYGYYDVIYETLNVDFIKACEKLIETGLIKEIDAMTDVTNGGVRGDAYEISKSSNVSLVFYKDRLYNTINEKVLNMLLELDIDPLGVSTDSLMVICNEDIADEVKKLINAIEVGYVEKGNESYIIEDNKKKPLKPMFRESAYTPVKKVVGEKKPKNFEKMKEMIRSACDEAIKKKDYVLELIRKRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTT4</accession>
    <name>N6VTT4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein TIM barrel</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01550</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589969.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTT4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00437"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.305"/>
    <dbReference type="OrthoDB" id="59344at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050312">
        <property type="entry name" value="IolE/XylAMocC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110">
        <property type="entry name" value="HYDROXYPYRUVATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110:SF21">
        <property type="entry name" value="XYLOSE ISOMERASE-LIKE TIM BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="1">
        <location>
            <begin position="21"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="265" mass="31840" checksum="F49E52518999FCEE" modified="2013-06-26" version="1">MRIGLSTLFFWEYPMFEIVDIFREIGLKCMEFFPENPDFWDNRFDLDYISELRREFKKFDIGLHAPHIELNPSSLNPYVREASIKETLWSLELAKFYRCDVITIHPGKRPTTREPTKEEYDHFYNYLNICLKEAKKKKITFCMENLPKKINRIGWHPKDVENILKNFEGLYLTLDFPHAKGYLDEFLELYDYIKHTHISGVINNRDHCSLIKSDIDFSKYILELLHRGYKGMFNLELDDRRFGKNLSKEEKINIVIKEVEYLESI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VUB7</accession>
    <name>N6VUB7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">molybdopterin molybdotransferase</fullName>
            <ecNumber evidence="4">2.10.1.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_00020</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>adenylyl-molybdopterin + molybdate = Mo-molybdopterin + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35047"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:36264"/>
            <dbReference type="ChEBI" id="CHEBI:62727"/>
            <dbReference type="ChEBI" id="CHEBI:71302"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.10.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MoeA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.10.1.1" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589697.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VUB7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01163"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.4"/>
    <dbReference type="OrthoDB" id="31371at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061599">
        <property type="term" value="F:molybdopterin molybdotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00887">
        <property type="entry name" value="MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.170.190.11:FF:000001">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.980.10:FF:000004">
        <property type="entry name" value="Molybdopterin molybdenumtransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.340.10:FF:000005">
        <property type="entry name" value="Molybdopterin molybdenumtransferase MoeA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.980.10">
        <property type="entry name" value="MoaB/Mog-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.340.10">
        <property type="entry name" value="MoeA, C-terminal, domain IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.105.10">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.190.11">
        <property type="entry name" value="Molybdopterin biosynthesis moea protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036425">
        <property type="entry name" value="MoaB/Mog-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001453">
        <property type="entry name" value="MoaB/Mog_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008284">
        <property type="entry name" value="MoCF_biosynth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038987">
        <property type="entry name" value="MoeA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005111">
        <property type="entry name" value="MoeA_C_domain_IV"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036688">
        <property type="entry name" value="MoeA_C_domain_IV_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005110">
        <property type="entry name" value="MoeA_linker/N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036135">
        <property type="entry name" value="MoeA_linker/N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045515">
        <property type="entry name" value="Glp_gephyrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00177">
        <property type="entry name" value="molyb_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011068">
        <property type="entry name" value="PRK14498.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192:SF5">
        <property type="entry name" value="GEPHYRIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10192">
        <property type="entry name" value="MOLYBDOPTERIN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00994">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03454">
        <property type="entry name" value="MoeA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03453">
        <property type="entry name" value="MoeA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12727">
        <property type="entry name" value="PBP_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00852">
        <property type="entry name" value="MoCF_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63867">
        <property type="entry name" value="MoeA C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63882">
        <property type="entry name" value="MoeA N-terminal region -like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53218">
        <property type="entry name" value="Molybdenum cofactor biosynthesis proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01079">
        <property type="entry name" value="MOCF_BIOSYNTHESIS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="9" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MoaB/Mog" evidence="11">
        <location>
            <begin position="183"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="610" mass="67878" checksum="98CC5B2D53F5FD1A" modified="2013-06-26" version="1">MRYLTLKNIEEAKKIVRDYLMGDVETVSIEEAVNRVLAEDVFSDIDIPPFDRATVDGYAVIAEDTFNADEDKPVELKVIDEIRAGEIKNIEIKRGECVEIATGAMIPKNANAVVMVEYTEREGDKVKIYKPIAPMENIQFAGSDVMVGELVLRKNTLLTPKDIGVLAAIGRKKVRVYKKLKFAIISTGNELVSPGEVLEDGKIYDINSYTLMSYIKNLGYDYVFLGIVRDNVEDIKEKINKGLGLADIILLSGGTSAGRGDLVEKAIEMLGGEILIHGLKIKPGKPTIIGKVKNKLVIGLPGYPTSCLTVFDVLFGNEKNLIKAKFRGRYISAKGRVEYLPVMLVKGKEYSCYPITKGSGAITTLSEADGYVVIDENREILEEEDVDVHLFGYTSALNIIGSHCIGIDIILKDANLQAKVINTGSLGGILAIKRGEADIAGIHLLDEKEGYNIPYLKKYNVKNAVLIRGYIREQGFILREDLSLDEIIKNIHNFEIISRNKGAGTRILFENFLKKHNINYNELKIVGEAKTHSSVAKAVAMGRADIGIGIKTVADYYKLKFIKIGEENFDFLIRKERFGDDDVQKFIRSLKRAKLPFKKPENCGEIIWEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VWX1</accession>
    <name>N6VWX1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Cobalamin (Vitamin B12) biosynthesis CbiM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_06997</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000067">
        <property type="protein sequence ID" value="ENN95592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594699.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VWX1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00578"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1355"/>
    <dbReference type="OrthoDB" id="71235at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000041">
        <property type="term" value="P:transition metal ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1760.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002751">
        <property type="entry name" value="CbiM/NikMN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008873">
        <property type="entry name" value="PRK11909.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34229">
        <property type="entry name" value="METAL TRANSPORT PROTEIN HI_1621-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34229:SF1">
        <property type="entry name" value="METAL TRANSPORT PROTEIN HI_1621-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01891">
        <property type="entry name" value="CbiM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="39"/>
            <end position="59"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="79"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="137"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="220" mass="23966" checksum="668298DA7A104D97" modified="2013-06-26" version="1">MHIPDGYLGPITCIFFYLLMIPFWYKGLKELRKLDPKKLPLLGVLTAFSFLVMMFNLPVPDGTTCHMVGGTLIAVLMDNPWVATIAISIVLIIQAIFFGDGGVTCIGANCFNMGVVLPFVGYYIYKFLATKVKIDEAVAAGIGAYFGLVIASVVAGFEFGIQPFIEPGYCPYPFTVSIPAMALAHMLVAGPVAAIVTGVIVWYVKKVRPELFAKKEVEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX54</accession>
    <name>N6VX54_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">SAM-dependent methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06366</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95692.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593518.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX54"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01300"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1239"/>
    <dbReference type="OrthoDB" id="30774at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052907">
        <property type="term" value="F:23S rRNA (adenine(1618)-N(6))-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070475">
        <property type="term" value="P:rRNA base methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010286">
        <property type="entry name" value="METTL16/RlmF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393:SF0">
        <property type="entry name" value="RNA N6-ADENOSINE-METHYLTRANSFERASE METTL16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13393">
        <property type="entry name" value="SAM-DEPENDENT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05971">
        <property type="entry name" value="Methyltransf_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95692.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="261" mass="30293" checksum="E39851198B617574" modified="2013-06-26" version="1">MLGLKIEEALKYNENLKKYVFEDKGKLKINFKDKKALMEYNRTVIKVLFGLDIEFHEKGLIPPVMSRYLFVKKSFETLERLGIKEAEVLEIGTGHSAIIAMLIKKLYNANVYATEVDKEFLEYARKNILKNNLKIELIDSKGKILKGIDEIKNKRFDLIISYPPYYSSNSKPSGRKFGGALAKEVELIGGGRFGEKFSFRLIEESYNFLKDKGVLSIMLPKKPDKRREIIIEKMNEYYEVEVDKIKLGNRERYVIKGIKDV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX85</accession>
    <name>N6VX85_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Zinc finger C2H2-type domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06219</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593342.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX85"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00294"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1211"/>
    <dbReference type="OrthoDB" id="63170at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036236">
        <property type="entry name" value="Znf_C2H2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013087">
        <property type="entry name" value="Znf_C2H2_type"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00096">
        <property type="entry name" value="zf-C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00355">
        <property type="entry name" value="ZnF_C2H2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57667">
        <property type="entry name" value="beta-beta-alpha zinc fingers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00028">
        <property type="entry name" value="ZINC_FINGER_C2H2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50157">
        <property type="entry name" value="ZINC_FINGER_C2H2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0862">Zinc</keyword>
    <keyword evidence="1" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="C2H2-type" evidence="2">
        <location>
            <begin position="16"/>
            <end position="39"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00042"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="46" mass="5480" checksum="0DFF9A03D8083759" modified="2013-06-26" version="1">MRLKAKKIESRYGKFLVCPRCGRVFKDSKAYTKHVNKSHRHLFKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX93</accession>
    <name>N6VX93_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Putative methylthioribose-1-phosphate isomerase</fullName>
            <shortName evidence="2">M1Pi</shortName>
            <shortName evidence="2">MTR-1-P isomerase</shortName>
            <ecNumber evidence="2">5.3.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="2">MTNA-like protein</fullName>
            <shortName evidence="2">aMTNA</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="2">S-methyl-5-thioribose-1-phosphate isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06279</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the interconversion of methylthioribose-1-phosphate (MTR-1-P) into methylthioribulose-1-phosphate (MTRu-1-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-(methylsulfanyl)-alpha-D-ribose 1-phosphate = 5-(methylsulfanyl)-D-ribulose 1-phosphate</text>
            <dbReference type="Rhea" id="RHEA:19989"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="ChEBI" id="CHEBI:58548"/>
            <dbReference type="EC" id="5.3.1.23"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the EIF-2B alpha/beta/delta subunits family. MtnA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.23" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95737.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593361.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX93"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00282"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1223"/>
    <dbReference type="OrthoDB" id="45195at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046523">
        <property type="term" value="F:S-methyl-5-thioribose-1-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.120.420:FF:000003">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.10470:FF:000006">
        <property type="entry name" value="Methylthioribose-1-phosphate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.420">
        <property type="entry name" value="translation initiation factor eif-2b, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10470">
        <property type="entry name" value="Translation initiation factor eif-2b, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01678">
        <property type="entry name" value="Salvage_MtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000649">
        <property type="entry name" value="IF-2B-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005251">
        <property type="entry name" value="IF-M1Pi"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042529">
        <property type="entry name" value="IF_2B-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011559">
        <property type="entry name" value="Initiation_fac_2B_a/b/d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027363">
        <property type="entry name" value="M1Pi_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00524">
        <property type="entry name" value="eIF-2B_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004326">
        <property type="entry name" value="PRK05720.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00512">
        <property type="entry name" value="salvage_mtnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43475:SF1">
        <property type="entry name" value="METHYLTHIORIBOSE-1-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01008">
        <property type="entry name" value="IF-2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="1 2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0486">Methionine biosynthesis</keyword>
    <keyword evidence="3" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton donor" evidence="2">
        <location>
            <position position="228"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="56"/>
            <end position="58"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="238"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="2">
        <location>
            <position position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95737.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="332" mass="37478" checksum="7237CAFF3BE88E2E" modified="2013-06-26" version="1">MKIKKKIKSEILLKPIIWEDNKLILIDQRKLPHKLEYYVCHNYKDVAFAIKEMVVRGAPAIGVAAAYGLALAEINEDDIEEAYKTLKNTRPTAINLFYALDRCMKAYKEGKSILEEAKRIHEEDIHVCKKIGEVGEKLIDDGDVILTHCNAGALATSMYGTALSVIRHAFWNGKKIIVIVDETRPRLQGALTAFELKYEGIPYKVITDNTAGFLMQKGEVDKIIVGADRILKNGTVYNKIGTYSLAVLAKYHDIPFYVAAPLSTFDLKSKEEDVIIEERDKKEVLYLKDILLFPEGSDCYNFAFDKTPAELITGIITEKGVVKPNEIMKLFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXV8</accession>
    <name>N6VXV8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Phosphoesterase RecJ domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_05000</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95972.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXV8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00489"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.980"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051319">
        <property type="entry name" value="Oligoribo/pAp-PDE_c-di-AMP_PDE"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47618">
        <property type="entry name" value="BIFUNCTIONAL OLIGORIBONUCLEASE AND PAP PHOSPHATASE NRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47618:SF1">
        <property type="entry name" value="BIFUNCTIONAL OLIGORIBONUCLEASE AND PAP PHOSPHATASE NRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DDH" evidence="1">
        <location>
            <begin position="11"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="DHHA1" evidence="2">
        <location>
            <begin position="235"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01368"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95972.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="36565" checksum="8FED731A5F70F736" modified="2013-06-26" version="1">MIEEYLKRDSILFLCHHNADPDAIGSAVALKYLSKYLNPNANVRIVADSISKISKNILNEINEKVDIELYPSLPETVFIVDTASINQLKVDFEELKKRDVILIDHHKRTELADICKYTIIKEDYPSTSEIVTEIFKELNIYPPKNIRIALLCGIVYDTKHLKLATQKTFEIISYLIKDISFQKILSMLTHESDISKRVAHLKACSRMRIKEYNGIKIALSHVSSYEASCAKTIVSIGADVAFVVAVRKKEGEIRVSARCRKHVAKKVHLGKLMEKIGKELKGSGGGHSEAGGLNAPYDGKDKEKTINRVLDLCYKRFIEEFKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYA9</accession>
    <name>N6VYA9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methanogen marker protein 4</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_04498</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MtxX family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96097.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591828.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYA9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00625"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.882"/>
    <dbReference type="OrthoDB" id="53227at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016764">
        <property type="entry name" value="MeTrfase_MtxX_xsu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03270">
        <property type="entry name" value="methan_mark_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019709">
        <property type="entry name" value="Methyltransf_MtxX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96097.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="239" mass="27325" checksum="50D9E070541E13DF" modified="2013-06-26" version="1">MIGIGLGKNKEEVIKAYNKLKEENIEVVLIEDYKTLIDKLLNKELSGAIRGSLPSSEVIPYLREKIGKFYRASILKNPFNNKLFLLSPVGIDDISIDKKERIDDKIRIIKYASNLLKKYEIEPKIGLLSGGRLEDRGRNIIVDETIEEAEEIKKNLKEYNILHYGILIERYLKEDCNIIVAIDGITGNLIFRSLALVAGLEGYGAILLSNKEIKFIDTSREANWKRYYNAVKFLLGEFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYD0</accession>
    <name>N6VYD0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Multiple resistance and pH regulation protein F</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04148</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000027">
        <property type="protein sequence ID" value="ENN96122.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591635.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYD0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01345"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.812"/>
    <dbReference type="OrthoDB" id="64746at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="32"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96122.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="87" mass="9199" checksum="C201B5406A1A529C" modified="2013-06-26" version="1">MNYFIWLFAIISLTFSALAGLRLAFKKGTANVLIGESIITVVIGTMIVVISEQYNIAFADTIALSLFISGIVGALAFAKIIGGDNGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYI7</accession>
    <name>N6VYI7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 11 12">5,10-methenyltetrahydromethanopterin hydrogenase</fullName>
            <ecNumber evidence="4 11 12">1.12.98.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 11">H(2)-dependent methylene-H(4)MPT dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 11">H(2)-forming N(5),N(10)-methylenetetrahydromethanopterin dehydrogenase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">N(5),N(10)-methenyltetrahydromethanopterin hydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">hmd</name>
        <name evidence="15" type="ORF">J422_03523</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to methylene-H(4)MPT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>5,10-methenyl-5,6,7,8-tetrahydromethanopterin + H2 = 5,10-methylenetetrahydromethanopterin + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20017"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:18276"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58337"/>
            <dbReference type="EC" id="1.12.98.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">One-carbon metabolism; methanogenesis from CO(2); 5,10-methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-tetrahydromethanopterin (hydrogen route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 11">Belongs to the HMD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.2" evidence="4 11 12"/>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96187.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591226.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYI7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01398"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.687"/>
    <dbReference type="OrthoDB" id="113982at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00696"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047068">
        <property type="term" value="F:N5,N10-methenyltetrahydromethanopterin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004735">
        <property type="term" value="F:pyrroline-5-carboxylate reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055129">
        <property type="term" value="P:L-proline biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1300">
        <property type="entry name" value="Hmd, C-terminal helical subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01090">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010062">
        <property type="entry name" value="HMD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004889">
        <property type="entry name" value="HMD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038182">
        <property type="entry name" value="HMD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055205">
        <property type="entry name" value="HMD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024190">
        <property type="entry name" value="METHMP_Hmd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01723">
        <property type="entry name" value="hmd_TIGR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11645:SF0">
        <property type="entry name" value="PYRROLINE-5-CARBOXYLATE REDUCTASE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03201">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22616">
        <property type="entry name" value="HMD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016158">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500165">
        <property type="entry name" value="HMDI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="6 11" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="8 11 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="5,10-methenyltetrahydromethanopterin hydrogenase N-terminal" evidence="14">
        <location>
            <begin position="1"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="domain" description="H2-forming N5,N10-methylenetetrahydromethanopterin dehydrogenase C-terminal" evidence="13">
        <location>
            <begin position="243"/>
            <end position="339"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00017649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR01723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF03201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF22616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN96187.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="343" mass="37727" checksum="31119A8EAC4B95E6" modified="2013-06-26" version="1">MKVAILGIGCYRTHAAAGITNFMRASEVANKVNMPEIALTHSSITMGAELLHLVDDVKEVIVSDPCFQENPGLVIIDEFDPKEVMEAHLRGEPESVMPKIREVVKQKAKELPKPPKACIHFVHPEDIGFKVAENDREAVEDADWVLTWLPKGKKQPDIIKNFVDAIPEGAIVTHACTIPTTKFAQIFKNFGREDLNITSYHPGCVPEMKGQVYIAEGYAKEDVLNKLFEMAKIARGNAYKMPANLIGPVCDMCSAVTAVIYAGLLAYRDSVTKILGAPADFAQMMAEESLKNILELMREKGITNMEDALDPGALLGTADSMCFGPLADILPQALKVLEKYKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYR0</accession>
    <name>N6VYR0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 14">S-adenosylmethionine synthase</fullName>
            <shortName evidence="14">AdoMet synthase</shortName>
            <ecNumber evidence="5 14">2.5.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 14">Methionine adenosyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">mat</name>
        <name evidence="16" type="ORF">J422_03373</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 14">Catalyzes the formation of S-adenosylmethionine from methionine and ATP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13 14">
            <text>L-methionine + ATP + H2O = S-adenosyl-L-methionine + phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21080"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="EC" id="2.5.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 14">Belongs to the AdoMet synthase 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.6" evidence="5 14"/>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96267.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590981.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYR0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01060"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.662"/>
    <dbReference type="OrthoDB" id="204488at2157"/>
    <dbReference type="UniPathway" id="UPA00315">
        <property type="reaction ID" value="UER00080"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004478">
        <property type="term" value="F:methionine adenosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.280">
        <property type="entry name" value="S-adenosylmethionine synthetase, C-terminal domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00136">
        <property type="entry name" value="S_AdoMet_synth2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027790">
        <property type="entry name" value="AdoMet_synthase_2_family"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042544">
        <property type="entry name" value="AdoMet_synthase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002795">
        <property type="entry name" value="S-AdoMet_synthetase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003364">
        <property type="entry name" value="PRK04439.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003366">
        <property type="entry name" value="PRK04439.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36697:SF1">
        <property type="entry name" value="S-ADENOSYLMETHIONINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01941">
        <property type="entry name" value="AdoMet_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="15" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="11 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 14" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 14 16" id="KW-0808">Transferase</keyword>
    <feature type="coiled-coil region" evidence="15">
        <location>
            <begin position="223"/>
            <end position="257"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="141"/>
            <end position="146"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00020319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00048344"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN96267.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="406" mass="45187" checksum="584E6DC3BF95684F" modified="2013-06-26" version="1">MRNIIVKELKDVPIEEKPIEIVERKGLGHPDSLADGIAEAVSKALCKMYMEKFGTILHHNTDQVEVVGGHAYPKFGGGEMVNPIYILLSGRATMQILDKEKNEIIKLPVGTVAVKAAKEYLRKVLRNIDVDKDVIIDCRIGQGSVDLVDVFERQKNKIPLANDTSFGVGYAPLSITEKLVLETERLLNSEETKKEIPALGEDIKVMGLRNGKKISLTIAAAIIDKYVRDIDEYKKVIEKIREKVEKLAEEIAKGYEVEIFINTADDYDRGVVYLTVTGTSAEMGDDGSVGRGNRVNGLITPFRPMSMEAASGKNPVNHVGKIYNILANLIANDISKLEGVKECYVRILSQIGKPINEPKALDIELILEEGYKIEDIEKKAKEIANKWLDNILEVQKMIIEGKVNTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYU1</accession>
    <name>N6VYU1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">TatD-related deoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03019</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96297.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590748.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYU1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00151"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.592"/>
    <dbReference type="OrthoDB" id="52767at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011589">
        <property type="entry name" value="UCP004961"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42206:SF1">
        <property type="entry name" value="METAL-DEPENDENT HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42206">
        <property type="entry name" value="METAL-DEPENDENT HYDROLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004961">
        <property type="entry name" value="UCP004961_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96297.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="266" mass="30360" checksum="88DEA2C05CD67A2B" modified="2013-06-26" version="1">MIITDNHIHVDNEKGYGAEKVAKIFYNAGGKVMIVLNKPTFDGDLTRSMDQLVRDVERINKNTEVKAFGLVGVHPAELTYLIKFMSLEEAKERIIKAMDYAKSLVENYDFIVGLGEVGRPHYKVSKEVWDASNDILKYCMTLAKDLSCPIQVHAESSERVFDDLYNMAKEVGLDTDKVIKHFCTDINDKIFPSILANKVNENIIKKSLRFVMETDYIDDLSRPGAVLGIKTVPRITKKLLEKGILDEEKVYKIHKENIEKLYDINL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYV6</accession>
    <name>N6VYV6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Probable cobyric acid synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cobQ</name>
        <name evidence="10" type="ORF">J422_03094</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the CobB/CobQ family. CobQ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96312.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590766.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYV6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00165"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.607"/>
    <dbReference type="OrthoDB" id="53136at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015420">
        <property type="term" value="F:ABC-type vitamin B12 transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05389">
        <property type="entry name" value="CobQ_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01750">
        <property type="entry name" value="GATase1_CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00028">
        <property type="entry name" value="CobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033949">
        <property type="entry name" value="CobQ_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047045">
        <property type="entry name" value="CobQ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004459">
        <property type="entry name" value="CobQ_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011698">
        <property type="entry name" value="GATase_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00313">
        <property type="entry name" value="cobQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001989">
        <property type="entry name" value="PRK00784.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343:SF1">
        <property type="entry name" value="COBYRIC ACID SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21343">
        <property type="entry name" value="DETHIOBIOTIN SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07685">
        <property type="entry name" value="GATase_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51274">
        <property type="entry name" value="GATASE_COBBQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="8">
        <location>
            <begin position="5"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="domain" description="CobB/CobQ-like glutamine amidotransferase" evidence="9">
        <location>
            <begin position="255"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="7">
        <location>
            <position position="330"/>
        </location>
    </feature>
    <feature type="active site" evidence="7">
        <location>
            <position position="431"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006205"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014921"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF07685"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96312.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="486" mass="55037" checksum="F23EF17F3D7730ED" modified="2013-06-26" version="1">MAEFIMVVGTSSNAGKSVLTAGICRLLANKGYKVAPFKSQNMSLNSRVAKEDGEIAVAQYTQSIAARVEPSVHFNPILLKPKGNYTSQVIVHGRPYGDMSYDEYRNNKNFFFKKIKESLEILNENYEYVVMEGAGSCCEINLLKDDIANLRVAEAVNAKAILVADIDRGGVFASIYGTIKLLPKNWRRLIKGIVINKFRGNKEVLKEGIDKIEELTGVPVIGILPYDEHFYLPEEDSLALQRLKSFGNKKSGVEVNVIRFKKISNFTDIDPLKYDSFIRLIDFCEEITGDILILPGSRGVTTEVKILKEYNFDEKIREFLKDGGIVIGICGGYQTLGRKLLDKNLKEGDVKDINGLNLLDIKTIFGNEKITKNSSGYYKDFLIKGYEIHEGYTYSKEKPFIKIVRGFGNCGDGYDGAIKKDRGLIVGTYFHGIFENSDFRNYILNIIRERKGLERIEGDKYKDEIERNLNYLAKTIDKHVNLRIIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ17</accession>
    <name>N6VZ17_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Isopropylmalate/isohomocitrate dehydrogenase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_02819</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the isocitrate and isopropylmalate dehydrogenases family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96372.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590624.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ17"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00113"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.553"/>
    <dbReference type="OrthoDB" id="6813at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003862">
        <property type="term" value="F:3-isopropylmalate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004449">
        <property type="term" value="F:isocitrate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019298">
        <property type="term" value="P:coenzyme B biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006102">
        <property type="term" value="P:isocitrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.718.10:FF:000019">
        <property type="entry name" value="Homoisocitrate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.718.10">
        <property type="entry name" value="Isopropylmalate Dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054960">
        <property type="entry name" value="HICDH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019818">
        <property type="entry name" value="IsoCit/isopropylmalate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024084">
        <property type="entry name" value="IsoPropMal-DH-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011828">
        <property type="entry name" value="LEU3_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040619">
        <property type="entry name" value="AksF_Meth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02088">
        <property type="entry name" value="LEU3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835">
        <property type="entry name" value="DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11835:SF34">
        <property type="entry name" value="ISOCITRATE DEHYDROGENASE [NAD] SUBUNIT ALPHA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00180">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01329">
        <property type="entry name" value="Iso_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53659">
        <property type="entry name" value="Isocitrate/Isopropylmalate dehydrogenase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00470">
        <property type="entry name" value="IDH_IMDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0520">NAD</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Isopropylmalate dehydrogenase-like" evidence="7">
        <location>
            <begin position="2"/>
            <end position="333"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96372.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="341" mass="37824" checksum="883E79597A7C44F6" modified="2013-06-26" version="1">MKICVIEGDGIGKEVIPEALKVLNLLGNFEIIKAEAGLECYKKYGYYLPKETIEKAKEADIILFGAVTSNPKDKNYKSPIITLRKLFDLYANVRPINNFGLGEIVGKIAGYKFLDIKSIDIVIIRENTEDLYVGREKENADKAVAERIITKKGAERIVRFAFEYAKDNNRKKVSCIHKANVLKITDGLFLETFYKISKNYNIEANDYLVDATAMHLIKNPNIFDVIVTTNMFGDILSDEASALIGGLGLVPSANIGENKALFEPVHGSAPDIAGKGIANPMAAILCVSMIFDYINENKKANLIREAIKYCLINGKTTPDLGGNLKTKEVGDEVIKYIKEMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ61</accession>
    <name>N6VZ61_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL22</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpl22</name>
        <name evidence="10" type="ORF">J422_02549</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">The globular domain of the protein is located near the polypeptide exit tunnel on the outside of the subunit, while an extended beta-hairpin is found that lines the wall of the exit tunnel in the center of the 70S ribosome.</text>
    </comment>
    <comment type="function">
        <text evidence="7 9">This protein binds specifically to 23S rRNA. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7 9">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uL22 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96417.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590467.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ61"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00763"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.499"/>
    <dbReference type="OrthoDB" id="314984at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00336">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.470.10:FF:000015">
        <property type="entry name" value="50S ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.470.10">
        <property type="entry name" value="Ribosomal protein L22/L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01331_A">
        <property type="entry name" value="Ribosomal_uL22_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001063">
        <property type="entry name" value="Ribosomal_uL22"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057265">
        <property type="entry name" value="Ribosomal_uL22_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018260">
        <property type="entry name" value="Ribosomal_uL22_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005721">
        <property type="entry name" value="Ribosomal_uL22_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036394">
        <property type="entry name" value="Ribosomal_uL22_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003260">
        <property type="entry name" value="PRK04223.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01038">
        <property type="entry name" value="uL22_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11593:SF10">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00237">
        <property type="entry name" value="Ribosomal_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54843">
        <property type="entry name" value="Ribosomal protein L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00464">
        <property type="entry name" value="RIBOSOMAL_L22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7 9" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009451"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96417.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="152" mass="17401" checksum="B93F0CCC910929BF" modified="2013-06-26" version="1">MGKIGYKIKVDDEKTAKAMGRNIPISRKHAREICREINGMPLDKAIKFLEDVIAMKRPVLFRRHCKKVGHRKGKLGWPAGRYPVKAAKAILKILLNAKSNAEYKGLNTDRLRIKHISTNKGITLKRYMPRAFGRATPKNQETVHVQVILEEY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZC7</accession>
    <name>N6VZC7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">F420-0--gamma-glutamyl ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_02245</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96472.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590280.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZC7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01197"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.441"/>
    <dbReference type="OrthoDB" id="11383at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052618">
        <property type="term" value="F:coenzyme F420-0:L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.100">
        <property type="entry name" value="CofE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1660.10">
        <property type="entry name" value="CofE-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008225">
        <property type="entry name" value="F420-0_g-glutamyl_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002847">
        <property type="entry name" value="F420-0_gamma-glut_ligase-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01916">
        <property type="entry name" value="F420_cofE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009809">
        <property type="entry name" value="PRK13293.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47917">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47917:SF1">
        <property type="entry name" value="COENZYME F420:L-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01996">
        <property type="entry name" value="F420_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144010">
        <property type="entry name" value="CofE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="1 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0464">Manganese</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-0630">Potassium</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Coenzyme F420:L-glutamate ligase-like" evidence="8">
        <location>
            <begin position="14"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96472.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="249" mass="27294" checksum="80D3FDF22C209E39" modified="2013-06-26" version="1">MIKEKNKIEIIGLELPIFKGNDITLEKLADIISQYPIKDYDIIVIAETLISKAEGNIIERDKIIPTKEAIEIAKKINKDPKVVQVILNEAKEIVKIGKNFIITETKHGFVCANSGVDESNVLGIKTLPKDPDKSAEVIRKYIEKKTKKKIAVIISDSVGRPFRKGAIGIAIGVSGIIALWDRRGEKDLFGRELKSTEVAVADLIASAANLAMGEANEGVPVVIIRGAKVPFGNGKASDLIRNKEEDVFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZD3</accession>
    <name>N6VZD3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase</fullName>
            <ecNumber evidence="7">2.1.1.282</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA wyosine derivatives biosynthesis protein Taw3</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">taw3</name>
        <name evidence="9" type="ORF">J422_02270</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">S-adenosyl-L-methionine-dependent methyltransferase that acts as a component of the wyosine derivatives biosynthesis pathway. Probably methylates N-4 position of wybutosine-86 to produce wybutosine-72.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36635"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="Rhea" id="RHEA-COMP:10379"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:73543"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.1.1.282"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the TYW3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.282" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96477.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590286.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZD3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01202"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.446"/>
    <dbReference type="OrthoDB" id="19299at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031591">
        <property type="term" value="P:wybutosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1960.10:FF:000010">
        <property type="entry name" value="tRNA(Phe) 7-((3-amino-3-carboxypropyl)-4-demethylwyosine(37)-N(4))-methyltransferase 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1960.10">
        <property type="entry name" value="tRNA wybutosine-synthesizing-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00266">
        <property type="entry name" value="TYW3_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022908">
        <property type="entry name" value="Taw3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003827">
        <property type="entry name" value="tRNA_yW-synthesising"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036602">
        <property type="entry name" value="tRNA_yW-synthesising-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003265">
        <property type="entry name" value="PRK04235.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047731">
        <property type="entry name" value="tRNAMtaseTaw3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48418:SF1">
        <property type="entry name" value="TRNA WYBUTOSINE-SYNTHESIZING PROTEIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02676">
        <property type="entry name" value="TYW3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111278">
        <property type="entry name" value="SSo0622-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="tRNA wybutosine-synthesizing protein" evidence="8">
        <location>
            <begin position="5"/>
            <end position="187"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008569"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00030554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96477.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="193" mass="22608" checksum="5515EF364CC3368C" modified="2013-06-26" version="1">MFSEDKRRVLMELNLAIEEGLVDREVIPILEKINNLKNYYTTSSCIGRIGILEIPKDKNPKIFSRWLGKWHDYPKIEDIINSLNKAKEGYILFVMNSPILHIACKDIESAKKMLELAIHNGLKASSIKSISKRRVIVEILTTYKIDTPIGVDGKVYVNYQYLKFLLDYAIKKLKMSRELIYRWADKLWEIERD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZT9</accession>
    <name>N6VZT9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01480</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000010">
        <property type="protein sequence ID" value="ENN96592.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589957.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZT9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01030"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.291"/>
    <dbReference type="OrthoDB" id="65298at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="47"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="81"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96592.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="101" mass="11569" checksum="94FCC62DA3E5E576" modified="2013-06-26" version="1">MDYDKLLKRLKDKDGITVGAYYTGDWSLYLMFIFGSIIVGRIYGNTMMVIISLMSLALLFSVPSLIFHFKKENSNNINTQLFWFSIFLGALALIIFIAKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>N6W044</accession>
    <name>N6W044_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Thermosome subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Chaperonin subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_00786</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Molecular chaperone; binds unfolded polypeptides in vitro, and has a weak ATPase activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Forms an oligomeric complex of eight-membered rings.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the TCP-1 chaperonin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96727.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589827.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W044"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00879"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.154"/>
    <dbReference type="OrthoDB" id="9362at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032991">
        <property type="term" value="C:protein-containing complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140662">
        <property type="term" value="F:ATP-dependent protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03343">
        <property type="entry name" value="cpn60"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.560.10:FF:000017">
        <property type="entry name" value="T-complex protein 1 subunit eta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.7.10:FF:000014">
        <property type="entry name" value="Thermosome subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.7.10">
        <property type="entry name" value="GroEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.560.10">
        <property type="entry name" value="GroEL-like equatorial domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.260.10">
        <property type="entry name" value="TCP-1-like chaperonin intermediate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017998">
        <property type="entry name" value="Chaperone_TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002194">
        <property type="entry name" value="Chaperonin_TCP-1_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002423">
        <property type="entry name" value="Cpn60/GroEL/TCP-1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027409">
        <property type="entry name" value="GroEL-like_apical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027413">
        <property type="entry name" value="GROEL-like_equatorial_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027410">
        <property type="entry name" value="TCP-1-like_intermed_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053374">
        <property type="entry name" value="TCP-1_chaperonin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054827">
        <property type="entry name" value="thermosome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012714">
        <property type="entry name" value="Thermosome_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041082">
        <property type="entry name" value="thermosome_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02339">
        <property type="entry name" value="thermosome_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041083">
        <property type="entry name" value="thermosome_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11353">
        <property type="entry name" value="CHAPERONIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00118">
        <property type="entry name" value="Cpn60_TCP1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00304">
        <property type="entry name" value="TCOMPLEXTCP1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52029">
        <property type="entry name" value="GroEL apical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48592">
        <property type="entry name" value="GroEL equatorial domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54849">
        <property type="entry name" value="GroEL-intermediate domain like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00750">
        <property type="entry name" value="TCP1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00751">
        <property type="entry name" value="TCP1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00995">
        <property type="entry name" value="TCP1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="3 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="10">
        <location>
            <begin position="524"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="10">
        <location>
            <begin position="524"/>
            <end position="534"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00063704"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00069477"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00083919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU004187"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96727.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="543" mass="59218" checksum="FC215B0DDE975B16" modified="2013-06-26" version="1">MSMAGTPIVVLPQNVKRYVGRDAQRMNILAGRIIAETVRTTLGPKGMDKMLVDELGDIVVTNDGVTILKEMSVEHPAAKMLIEVAKTQEKEVGDGTTTAVVIAGELLRKAEELLDQNIHPSVIINGYEIARNKAIEELKKIAKEVKPDDKEMLKKIAMTAITGKGAEKAREKLAEIVVEAVSTVIDDETGKVEKDLIKVEKKEGAPIEETTLIRGVVIDKERVHPQMPKRVENAKIALLNCPIEVKETEIDAEIRITDPAKIMEFVEQEEKMIKEMVDKIAATGANVVFCQKGIDDLAQHYLAKKGILAVRRVKKSDMEKLAKATGARIVTKIDDLTPEDLGEAGLVEERKVAGDAMIFVENCKHPKAVTILARGSTEHIVEEVARAIDDAIGVVKCALEDGRIVVGGGATEIELAKRISKFAETVAGREQLAVKAFAEALEIIPRTLAENSGLDPIDMLVKLRAKHEEEGGEVYGLDVFEGEVVNMLERGVVEPLKVKTQAIDSATEASIMLLRIDDVIAAEKSEKEKEKGPGGEEEGGDEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6W058</accession>
    <name>N6W058_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">THUMP domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00861</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96742.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589842.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W058"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00862"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.170"/>
    <dbReference type="OrthoDB" id="64018at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002937">
        <property type="term" value="P:tRNA 4-thiouridine biosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd11718">
        <property type="entry name" value="THUMP_SPOUT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025849">
        <property type="entry name" value="MJ0421-like_SPOUT_MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041730">
        <property type="entry name" value="MJ0421-like_THUMP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050102">
        <property type="entry name" value="tRNA_sulfurtransferase_ThiI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209:SF1">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14419">
        <property type="entry name" value="SPOUT_MTase_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="45"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96742.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="359" mass="41720" checksum="85B0175B2EF6823D" modified="2013-06-26" version="1">MKFIIKTERGLENIVVNHLKDLVENFNYITSPDGYQGIVIVEAEEDIRDKILQIPEVDSVLKVDYEVETDFEKIVNMAEKIKEHINENETFAVETKKRGKQDFNSVDVNIVLGAKLKDLTNAEVNLTNPDKVIYVEVFKHKTYICIGKKDEYKGKKYTKEKINARTLFKKISIVQLPYLEDIKACRKFGEAIGRSAQAFEVKELIIAPKDKVDAYELMEFIKGVKIGQISRYEIQKKAYPFEIKKVPVYVHDLYQTVRDKRRNNRLLIITDPKGEEIIKIKDKLKEDLKKKKEIVVFCGSREGIPKGVFRFADYIIDLSPHMTFATEHAIPAALIALWTVFRESFDDDYEREDDKNIDQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6W0E1</accession>
    <name>N6W0E1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00305</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589748.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W0E1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01103"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.61"/>
    <dbReference type="OrthoDB" id="18264at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07391">
        <property type="entry name" value="MPP_PF1019"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004376">
        <property type="entry name" value="Pesterase_MJ0037"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024173">
        <property type="entry name" value="Pesterase_MJ0037-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00024">
        <property type="entry name" value="SbcD_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39323:SF1">
        <property type="entry name" value="BLR1149 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000887">
        <property type="entry name" value="Pesterase_MJ0037"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="37"/>
            <end position="194"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="241" mass="28007" checksum="339E447D0119CAD0" modified="2013-06-26" version="1">MKYPGFPRADTNFFRESIMKIKNLTLTEDRAIIYKKYGIIADTHIGFDISFNESGANFPLIQKDRVINNILKLIENYKIKSLIINGDLKHNFYPIKREIEFVNEFLNLLNEHVELILIKGNHDTYLSKITETYDYFELGKYTVTHGHKVVEGDFLILAHEHPSIKIRDEITTYKFPIYLVNKNYIVLPAFNPLSPGNDLVNNYPSSPIIKKDYLDGEVFGITEIGLLEFGKLKDLLEFNRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTS2</accession>
    <name>N6UTS2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cobalamin biosynthesis precorrin-3 methylase CbiF</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_06070</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the precorrin methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95744.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593229.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTS2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00533"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1182"/>
    <dbReference type="OrthoDB" id="6633at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046026">
        <property type="term" value="F:precorrin-4 C11-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd11641">
        <property type="entry name" value="Precorrin-4_C11-MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006362">
        <property type="entry name" value="Cbl_synth_CobM/CibF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006366">
        <property type="entry name" value="CobA/CysG_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050161">
        <property type="entry name" value="Siro_Cobalamin_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003043">
        <property type="entry name" value="Uropor_MeTrfase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01469">
        <property type="entry name" value="cobA_cysG_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01465">
        <property type="entry name" value="cobM_cbiF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790:SF4">
        <property type="entry name" value="COBALT-PRECORRIN-4 C(11)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45790">
        <property type="entry name" value="SIROHEME SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00839">
        <property type="entry name" value="SUMT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00840">
        <property type="entry name" value="SUMT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4 7 9" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="8">
        <location>
            <begin position="3"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95744.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="249" mass="27557" checksum="B06372DBA5CEFB58" modified="2013-06-26" version="1">MKKVTIVGAGPGDEELITVKGLKAIKEADVIIYAGSLINKKLLEYNKKAELYDSSKMSLDEIIDVMVKAVNEGKKVVRLHSGDPSIYGAIKEQIDELKKYNIDVEIIPGVSSLFAAAAALKIELTLPNVSQTVIITRPEGRTKVPEKLRELAKHRATMAIFLGVSMIDKVVEELLNGYDPSTPVAVVYHASWEDEKIIRGTLGDIVEKVKKENITRSALILVGDVLDPKNYEYSKLYDKTFSHGFRKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTX2</accession>
    <name>N6UTX2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05883</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000045">
        <property type="protein sequence ID" value="ENN95799.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592900.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTX2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00847"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1147"/>
    <dbReference type="OrthoDB" id="87890at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95799.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="152" mass="18092" checksum="2046586B4DDB55C5" modified="2013-06-26" version="1">MESKNTQYMVKLKYIKPIKLPIVKEQKLVEFIPIIPLEDDKYIKGKIEGAARVFLPEFINFAKHLGFEVSGKKRITLKTDDDYAYLRLFVYAMVMQALKDNTMWGKLEDHILSMEPLALRYWASTFRNNYWEHNNRRKLLKIAKCFLDVEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU65</accession>
    <name>N6UU65_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Xylose isomerase-like TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05299</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95894.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592373.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU65"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01327"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1034"/>
    <dbReference type="OrthoDB" id="372143at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050312">
        <property type="entry name" value="IolE/XylAMocC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110">
        <property type="entry name" value="HYDROXYPYRUVATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110:SF21">
        <property type="entry name" value="XYLOSE ISOMERASE-LIKE TIM BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="1">
        <location>
            <begin position="25"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95894.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="265" mass="30063" checksum="E10848E2905186FE" modified="2013-06-26" version="1">MKIAVSSLVFLPESLESVLEKIESFEAWEIVCEGTHYLNSKNIKYLLEIKDSYDISLLVHAPFSDLNPASMNAKVRELTVECVRDAIEGAFELDAELVVVHPGYVPELWKEYKDEILDNNFSTLLKIVEIAEDYNIKIGLENMPNFTGVLGKTPGEMLGIIKDIDSKVLGITFDVGHANTVSDPIKLLEDFAKLNIIHIHAHDNNGYSDDHLKIGEGNIDYFSVIKKLHEMRYDGYITIENKNLRDAMKSKEILEEYISMVNKIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU70</accession>
    <name>N6UU70_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">polynucleotide 5'-hydroxyl-kinase</fullName>
            <ecNumber evidence="2">2.7.1.78</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_05324</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of both single-stranded RNA (ssRNA) and single-stranded DNA (ssDNA). Exhibits a strong preference for ssRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>a 5'-end dephospho-2'-deoxyribonucleoside-DNA + ATP = a 5'-end 5'-phospho-2'-deoxyribonucleoside-DNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15669"/>
            <dbReference type="Rhea" id="RHEA-COMP:13180"/>
            <dbReference type="Rhea" id="RHEA-COMP:13184"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:136412"/>
            <dbReference type="ChEBI" id="CHEBI:136416"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>a 5'-end dephospho-ribonucleoside-RNA + ATP = a 5'-end 5'-phospho-ribonucleoside-RNA + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:54580"/>
            <dbReference type="Rhea" id="RHEA-COMP:13936"/>
            <dbReference type="Rhea" id="RHEA-COMP:15179"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:138282"/>
            <dbReference type="ChEBI" id="CHEBI:138284"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.78"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.78" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95899.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592378.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU70"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01322"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1039"/>
    <dbReference type="OrthoDB" id="359472at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051734">
        <property type="term" value="F:ATP-dependent polynucleotide 5'-hydroxyl-kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045116">
        <property type="entry name" value="Clp1/Grc3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032319">
        <property type="entry name" value="CLP1_P"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755">
        <property type="entry name" value="CLEAVAGE/POLYADENYLATION FACTOR IA SUBUNIT CLP1P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12755:SF3">
        <property type="entry name" value="POLYNUCLEOTIDE 5'-HYDROXYL-KINASE NOL9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16575">
        <property type="entry name" value="CLP1_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0418">Kinase</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Clp1 P-loop" evidence="10">
        <location>
            <begin position="34"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012157"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00024737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00044641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00044673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF16575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95899.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="341" mass="39357" checksum="10764B5EF835B830" modified="2013-06-26" version="1">MINKASYPESVLEDRFELLDYIKEHDKRKIILVGGVDTGKTTLATFLIKNLKYNLIDADVGQKSILPPATVSLGNYKEGFLCLSEIKEEKSYFIGSTNPTQFIGEMLTGVKKLADYAKEYFIIDTTGLINGAGEYLKKMKIELVEPDLIVALQKSNELSHILKPFIDKVDIFYIKPYIGKKYSREERKDIRENKWKTYFNNAKEIEINFEDYIISGSKAFQGERITDDEKLLLENIYKWEILYGSKCEGKFFIVKKDVKVVKRSVDKNLIFYMEPERFNNLVVGLIDNEGFCSGLAIIKEIDFKNESLKLITPSSLKNVREIRLGRVKLENGERLIDKDYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUC2</accession>
    <name>N6UUC2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Lysine--tRNA ligase</fullName>
            <ecNumber evidence="10">6.1.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Lysyl-tRNA synthetase</fullName>
            <shortName evidence="10">LysRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">lysK</name>
        <name evidence="10" type="synonym">lysS</name>
        <name evidence="12" type="ORF">J422_04860</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>tRNA(Lys) + L-lysine + ATP = L-lysyl-tRNA(Lys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20792"/>
            <dbReference type="Rhea" id="RHEA-COMP:9696"/>
            <dbReference type="Rhea" id="RHEA-COMP:9697"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78529"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.6"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.6" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95944.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592060.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUC2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00518"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.952"/>
    <dbReference type="OrthoDB" id="6838at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004824">
        <property type="term" value="F:lysine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006430">
        <property type="term" value="P:lysyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00674">
        <property type="entry name" value="LysRS_core_class_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.350">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.20.10">
        <property type="entry name" value="Lysine tRNA ligase, stem contact fold domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00177">
        <property type="entry name" value="Lys_tRNA_synth_class1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045462">
        <property type="entry name" value="aa-tRNA-synth_I_cd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020751">
        <property type="entry name" value="aa-tRNA-synth_I_codon-bd_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008925">
        <property type="entry name" value="aa_tRNA-synth_I_cd-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002904">
        <property type="entry name" value="Lys-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042078">
        <property type="entry name" value="Lys-tRNA-ligase_SC_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00467">
        <property type="entry name" value="lysS_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37940:SF1">
        <property type="entry name" value="LYSINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19269">
        <property type="entry name" value="Anticodon_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01921">
        <property type="entry name" value="tRNA-synt_1f"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48163">
        <property type="entry name" value="An anticodon-binding domain of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 10" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="6 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 10 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class I anticodon-binding" evidence="11">
        <location>
            <begin position="433"/>
            <end position="520"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="10">
        <location>
            <begin position="28"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="10">
        <location>
            <begin position="275"/>
            <end position="279"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF19269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95944.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="523" mass="61270" checksum="C2BCAB476B1E02A5" modified="2013-06-26" version="1">MFWADVIADKLLKDRKEEKYVIATGITPSGHIHIGNARETLTGDAIYKALKEKADAELIFIADTYDPLRKRYPFLPEEFEEYVGMPLSEIPCPEGCCKSYADHFLNPYLESLDDLGVELKVYKADENYKKGLYDEKIRIALEKRDKIREILNKFRDNPLDDSWFPINVVCEKCKNLKTKVIGYEGEEVYYRCEKCGHEGKVKPYKGKAKLPWRVDWPARWSIFNVSIEPMGKDHAAAGGSYDTGYLIAKEVFNYNPPMKVVYEWIQLKVGDKAIPMSSSKGVVFAVKDWTYVAHPEILRFLILRSKPSKHIDFDMKKIPDLVDEYDRLEEFYFENRDKDLDEDGKEKIRIYEFSTPRIPEKKPFVIPYRFCSIIGQLAYEDNNINMNKVFEILKRNNYNIDEIDEFSLKRLKDRLIMARNWALKYGEKLIIISDDEAKKEFETLKDKQKEWIRYFAERLENVNFDALTIHELIYETAKEIGLNPRDAFQAAYKILLGKKYGPRLGAFLASLGRDFVIKRFSMF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUH6</accession>
    <name>N6UUH6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Methyltransferase type 11 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05110</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95994.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592156.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUH6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00467"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1002"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050508">
        <property type="entry name" value="Methyltransf_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="46"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95994.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="216" mass="26180" checksum="E56A202A77D0AB9A" modified="2013-06-26" version="1">MDIEEFYDNWNPEEFPDYIKILMEFADRLIKNEIKKLLVEEPKFVLDCGCGFGAFYELTKDHNTLYLDISFYQLKKFKIKEKKICSNMLYLPFKNEVFDLILCINVLEHVNYKRALEEMFRVLKKGGRIIVVVINKNRLINEEIFTEFKIYHQPLSIKDFYNINFYTSVYFLPSFFKILPSRILKVFLKYWIFVDRFLSKLFKDKGLFLIIEMVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUL9</accession>
    <name>N6UUL9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04595</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000029">
        <property type="protein sequence ID" value="ENN96034.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591915.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUL9"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.900"/>
    <dbReference type="OrthoDB" id="386214at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96034.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="1073" mass="126597" checksum="DE497EACDF87E837" modified="2013-06-26" version="1">MLSYVKNSIKNLPPRKYTIEELKELNKQKLEALRKYHNSNLFVWLAKGFKGKNEDDAKRPITLTLDKEDLKSDELKLLRDLNIDTTRDEIKITHGIIKKLINKGYSKLPQFVFDALYRVLEQNPEYNICVEPIGEVVAYDLDSDDYFEIFKQAYYEVFGEDVLKDTFVEKTKQGYHIFLRLTNFIPNGRTIEFITKKEGKEFARYYNGKYEIVYPSIRVTNPEELEGIPYTKLSTKGLDELKTISLDKLKELEIKIVELLEKKGYKVRNEVKRLLGIPIGENGRLHIGVEGTNSKATIEVMPKNKEELLNTILEDVKKLQIEKGERSDWIFALTLLFKWCGLSCEDAFNILTSLEGIKTKFEDSSHNLDWYKTYEWNAAENVNLAGIIGAFQQCPKIDKTNILEILESYKEKGGVLYNKEFYREVKELKKKIIKSIYYMVIAKEKRHYVFKFNIYKIVIDLKKKKELMAEGKEEKEALEEATTKEIIEYPIFKFMDLNQFRDIILNKNYVVVKGITTIRNNNLKNEYELRFEDITGLLNYVSTDATREVIKKVKNALDVLIDAIEWFANNKTYESSCLFRVLERDGKLTIPKVEHHPILHQYESYPTPEEYYKMLMETTYTEEEKKQLLETLYKLNKPHNVLVMAYFLASLLVGVVDYEITPFLILYGKSGKGKSKTARIFNFVDIKTDRLTEYQYKVYANGWGCGFGLLDECKELRNELIQLLKENATSPIYDKKHGQKYRHIYKLCGILTCNDIFDLKTNNPDDLAGFLRRQLTYKVRDEDIIEGIGKDIKYLLKNRLKLQKIFIDWILKQDPQELREVYETIEEEEQYKFIYFGLGLLFKFWADNGFDVNKEYYKEILRMLKAGEQEFKKDVMNDEGIIDILHEVVYNRLLELVKSIPWGDKKVSIEELEDWDILNHYAIELMKKEGYTIYRLEREDKIRIAIQQRGLIKLLSVLKKRFKMNYPDKITLEWFANKLREDHEDHNIEIKQIKISGKKFYKCLVIDLKEEIEEQDLKEEVKKIVEEAGIRGMTTTEVCDRLGLSEDIVERVLKELHREGEIDNPRPNIWIRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUR9</accession>
    <name>N6UUR9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9 10">A-type ATP synthase subunit I</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_04398</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the V-ATPase 116 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96079.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUR9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00644"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.862"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016471">
        <property type="term" value="C:vacuolar proton-transporting V-type ATPase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051117">
        <property type="term" value="F:ATPase binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007035">
        <property type="term" value="P:vacuolar acidification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1460.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2750">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002490">
        <property type="entry name" value="V-ATPase_116kDa_su"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004428">
        <property type="entry name" value="PRK05771.2-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629:SF63">
        <property type="entry name" value="V-TYPE PROTON ATPASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11629">
        <property type="entry name" value="VACUOLAR PROTON ATPASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01496">
        <property type="entry name" value="V_ATPase_I"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="379"/>
            <end position="403"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="415"/>
            <end position="438"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="467"/>
            <end position="490"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="502"/>
            <end position="523"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="529"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="553"/>
            <end position="571"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="602"/>
            <end position="624"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="110"/>
            <end position="140"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="237"/>
            <end position="272"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00059506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00068671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU361189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96079.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="665" mass="75183" checksum="22E31918EC710083" modified="2013-06-26" version="1">MKKLKMVILDEKVDDVVRALHDHGLVEICDISEKLENSEWKNLLSVSHSADYVRDVMVMLIKSSRLLDLFSSVKEEKSSIKELLNPEPPEKKKVNFKTYKEVLDYAKKVLDEISKEADPLANKLNEIENKKSKMIQLKEQISYLKGLDFDLKYLGEGAFAYIGAGNVPKEKLDELKENLEKVTDGYVEVFSGEVFEKEGKQRVPIVFITLKEYLDTVLSELRKFEFERYDIKAEGYVKDVLANIERELNNIEKEKENIVREIRNLANKYEKELLVVHELLTIEKERGEVYNHFGKTERTYYLEAWVPEKYAKKAKDIIEKSSNGFCFVEITDPDEPEERIPVMLDNPKPIKPFEMLTEMYAMPKYNEVDPTLLIVPGFLLFYGIMLTDAVYGFLLTIVGLWVWKRIGKVSEGARNLGYILTLAGISTIIMGIITGGYLGDFLWEFLKIDIYKEGLALVNPLGGNGPIMILTFAIAVGVLHLFIGLLVGFIENIKKGKIGDAIFHQGIWLFLIIALVIGLLFPIMPIYKIPAIILIALIIVIIMCAIKGFKENGLMGALLGALDITGFLGNVLSYARLLALCLATGGLAMAVNIMAKLIGGAVPILGIILTIIVLFIGHIFNFVMNGLGAFIHSLRLHYVEFFSQFYEGGGKKFKPFKAQREYTTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUS2</accession>
    <name>N6UUS2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">A-type ATP synthase subunit A</fullName>
            <ecNumber evidence="13">7.1.2.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">atpA</name>
        <name evidence="18" type="ORF">J422_04423</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane. The A chain is the catalytic subunit.</text>
    </comment>
    <comment type="function">
        <text evidence="1">Produces ATP from ADP in the presence of a proton gradient across the membrane. The archaeal alpha chain is a catalytic subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>ATP + H2O + 4 H(+)(in) = ADP + phosphate + 5 H(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:57720"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.1.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="13">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 13">Cell membrane</location>
            <topology evidence="2 13">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 13">Belongs to the ATPase alpha/beta chains family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.1.2.2" evidence="13"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96084.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591800.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUS2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00639"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.867"/>
    <dbReference type="OrthoDB" id="115235at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033178">
        <property type="term" value="C:proton-transporting two-sector ATPase complex, catalytic domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18111">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18119">
        <property type="entry name" value="ATP-synt_V_A-type_alpha_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01134">
        <property type="entry name" value="V_A-ATPase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000675">
        <property type="entry name" value="V-type ATP synthase alpha chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1140.10:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.20:FF:000002">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.100:FF:000008">
        <property type="entry name" value="V-type proton ATPase catalytic subunit A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1140.10">
        <property type="entry name" value="Bovine Mitochondrial F1-atpase, Atp Synthase Beta Chain, Chain D, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00309">
        <property type="entry name" value="ATP_synth_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055190">
        <property type="entry name" value="ATP-synt_VA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031686">
        <property type="entry name" value="ATP-synth_a_Xtn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023366">
        <property type="entry name" value="ATP_synth_asu-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005726">
        <property type="entry name" value="ATP_synth_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020003">
        <property type="entry name" value="ATPase_a/bsu_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004100">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036121">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000194">
        <property type="entry name" value="ATPase_F1/V1/A1_a/bsu_nucl-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024034">
        <property type="entry name" value="ATPase_F1/V1_b/a_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022878">
        <property type="entry name" value="V-ATPase_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01043">
        <property type="entry name" value="ATP_syn_A_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003220">
        <property type="entry name" value="PRK04192.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607:SF1">
        <property type="entry name" value="H(+)-TRANSPORTING TWO-SECTOR ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43607">
        <property type="entry name" value="V-TYPE PROTON ATPASE CATALYTIC SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00006">
        <property type="entry name" value="ATP-synt_ab"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02874">
        <property type="entry name" value="ATP-synt_ab_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16886">
        <property type="entry name" value="ATP-synt_ab_Xtn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22919">
        <property type="entry name" value="ATP-synt_VA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47917">
        <property type="entry name" value="C-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50615">
        <property type="entry name" value="N-terminal domain of alpha and beta subunits of F1 ATP synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00152">
        <property type="entry name" value="ATPASE_ALPHA_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="8 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 13" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 13" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="18" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 13" id="KW-0406">Ion transport</keyword>
    <keyword evidence="11 13" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 13" id="KW-1278">Translocase</keyword>
    <keyword evidence="4 13" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit N-terminal" evidence="15">
        <location>
            <begin position="5"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="ATPsynthase alpha/beta subunit barrel-sandwich" evidence="16">
        <location>
            <begin position="108"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase F1/V1/A1 complex alpha/beta subunit nucleotide-binding" evidence="14">
        <location>
            <begin position="211"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="ATP synthase A/B type C-terminal" evidence="17">
        <location>
            <begin position="442"/>
            <end position="543"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="231"/>
            <end position="238"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003912"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF00006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF02874"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF16886"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF22919"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ENN96084.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="584" mass="65417" checksum="7B87861E9E054DFE" modified="2013-06-26" version="1">MKGKIVKIAGPVVVAEGMKGAQMYEVVKVGEEKLTGEIIQLHGDRVTIQVYEETTGVKPGEPVIGTGAPLSVELGPGMLKAMYDGIQRPLTAIEEKTGSIFIPRGVDVPALPRDIKWEFKPIVNEGAYVEEGDILGIVDETPSIKHKILVPIGIRGKVIEIKEGKFTVDETVAIIETENGEEEIKMMQKWPVRKPRPYKEKLPPRIPMITGQRVEDTFFPIAKGGTAAIPGPFGSGKTVTQHQLAKWSDADVVVYIGCGERGNEMTEVIEEFPHLEDIRTGNKLMDRTVLIANTSNMPVAAREASVYTGITIAEYFRDMGYGVLLTADSTSRWAEAMREISGRLEEMPGEEGYPAYLASRLAQFYERAGRVICLGQDNREGFVSIVGAVSPPGGDFSEPVTSNTLRIVKVFWALDANLARRRHFPAINWLLSYSLYVDEVTDWWHKNVGNDWRLLRDTAMSLLQKEAELQEIVQLVGPDALPDRERVILEVARMLREDFLQQDAFDEVDTYCPPKKQYLMLKIIMTFYEEALKAVERGVEPKKIISVPVKQEIARMKYMPHDEFINVKANEILNKVKEQLSSLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUT7</accession>
    <name>N6UUT7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Crossover junction endodeoxyribonuclease Hjc</fullName>
            <shortName evidence="11">Hjc</shortName>
            <ecNumber evidence="11">3.1.21.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Holliday junction resolvase Hjc</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">hjc</name>
        <name evidence="12" type="ORF">J422_04568</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">A structure-specific endonuclease that resolves Holliday junction (HJ) intermediates during genetic recombination. Cleaves 4-way DNA junctions introducing paired nicks in opposing strands, leaving a 5'-terminal phosphate and a 3'-terminal hydroxyl group that are subsequently ligated to produce recombinant products.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).</text>
            <dbReference type="EC" id="3.1.21.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the Holliday junction resolvase Hjc family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.21.10" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96109.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591852.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUT7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00612"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.896"/>
    <dbReference type="OrthoDB" id="34330at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008821">
        <property type="term" value="F:crossover junction DNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00523">
        <property type="entry name" value="Holliday_junction_resolvase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1350.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01490">
        <property type="entry name" value="HJ_Resolv_Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002732">
        <property type="entry name" value="Hjc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014428">
        <property type="entry name" value="Hjc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011856">
        <property type="entry name" value="tRNA_endonuc-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040854">
        <property type="entry name" value="Hol_resolv_Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE HJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39651:SF1">
        <property type="entry name" value="HOLLIDAY JUNCTION RESOLVASE HJC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01870">
        <property type="entry name" value="Hjc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004985">
        <property type="entry name" value="Hlld_jn_rslvs_ar"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8 11" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="9 11" id="KW-0234">DNA repair</keyword>
    <keyword evidence="7 11" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" evidence="11">
        <location>
            <position position="30"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="47"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="11">
        <location>
            <position position="49"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00029354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96109.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="120" mass="13934" checksum="E4E51FF07CA727B5" modified="2013-06-26" version="1">MSYKKGSSFERKLKELLEKEGFAVIRSAGSKGIDLIAGKNGKILIFECKSSNKNRFYINREDVEKLIKFSEIFGGKPYLAVKMQRKIFFIDPYLLSTNKKSFVIDKNIENFSLTFYDIIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUY0</accession>
    <name>N6UUY0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">AsnC family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_03878</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96164.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591376.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUY0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00406"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.760"/>
    <dbReference type="OrthoDB" id="6995at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000485">
        <property type="entry name" value="AsnC-type_HTH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011008">
        <property type="entry name" value="Dimeric_a/b-barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050684">
        <property type="entry name" value="HTH-Siroheme_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019888">
        <property type="entry name" value="Tscrpt_reg_AsnC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019887">
        <property type="entry name" value="Tscrpt_reg_AsnC/Lrp_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019885">
        <property type="entry name" value="Tscrpt_reg_HTH_AsnC-type_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413:SF7">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR PTR2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43413">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ASNC FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01037">
        <property type="entry name" value="AsnC_trans_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13404">
        <property type="entry name" value="HTH_AsnC-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00033">
        <property type="entry name" value="HTHASNC"/>
    </dbReference>
    <dbReference type="SMART" id="SM00344">
        <property type="entry name" value="HTH_ASNC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54909">
        <property type="entry name" value="Dimeric alpha+beta barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00519">
        <property type="entry name" value="HTH_ASNC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50956">
        <property type="entry name" value="HTH_ASNC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH asnC-type" evidence="4">
        <location>
            <begin position="2"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50956"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96164.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="141" mass="16127" checksum="2D909BA83CF2F0D7" modified="2013-06-26" version="1">MLDEKDRKILEILMKDARKPFTEIAKELNTSESSIRKRIKKMEENGIIKGYTVVIEPAKIGYNVIALTGFDTDPQDVLDVAKELCKFEEVKKVFTSTGDHMIMTEIWARDGSELKEILFNKIGKIKGIKKICPAIILERLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVC6</accession>
    <name>N6UVC6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">SMC domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03079</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96309.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVC6"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.604"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96309.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="47" mass="5641" checksum="590B55A1580C7CA2" modified="2013-06-26" version="1">MAKLIEIFRKIRNIPQMIVITHHKEIEEVADNIIRVYKEDYSKVSVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVZ1</accession>
    <name>N6UVZ1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Pyruvate synthase subunit PorC</fullName>
            <ecNumber evidence="2">1.2.7.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Pyruvate oxidoreductase gamma chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">Pyruvic-ferredoxin oxidoreductase subunit gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_01705</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>2 oxidized [2Fe-2S]-[ferredoxin] + pyruvate + CoA = 2 reduced [2Fe-2S]-[ferredoxin] + acetyl-CoA + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12765"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="1.2.7.1"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.1" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96494.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590099.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVZ1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00192"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.335"/>
    <dbReference type="OrthoDB" id="372091at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019164">
        <property type="term" value="F:pyruvate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051626">
        <property type="entry name" value="Oxidoreductase_gamma_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053412">
        <property type="entry name" value="Pyruvate_synthase_PorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040683">
        <property type="entry name" value="PorC_Meth_Thtga"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006321">
        <property type="entry name" value="PRK08534.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43366">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43366:SF1">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="8">
        <location>
            <begin position="10"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00019586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00044813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00044815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049357"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96494.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="178" mass="19524" checksum="1798B9B0409B9466" modified="2013-06-26" version="1">MIEIRFHGRGGQGAVTAARILAKAAFYDGKYCQAFPFFGVERRGAPVKAFTRIDDKPIRLRCQIYEPDYIVVQDSTLLDVVNVFEGLKKDGAVIINTVKDMEFDYKTYCIDATGIALEVLGVPIVNTAMVGAFAGATKLVSLESIKKAILETFSGKLGEKNALAAEKAYNEIIKKYGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWF1</accession>
    <name>N6UWF1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell division ATPase MinD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_01081</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96644.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589884.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWF1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00826"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.211"/>
    <dbReference type="OrthoDB" id="31168at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02036">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000285">
        <property type="entry name" value="Sporulation initiation inhibitor Soj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002586">
        <property type="entry name" value="CobQ/CobB/MinD/ParA_Nub-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010224">
        <property type="entry name" value="MinD_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01969">
        <property type="entry name" value="minD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF10">
        <property type="entry name" value="ATPASE INVOLVED IN CHROMOSOME PARTITIONING, PARA_MIND FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01656">
        <property type="entry name" value="CbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobQ/CobB/MinD/ParA nucleotide binding" evidence="4">
        <location>
            <begin position="5"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="11"/>
            <end position="18"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR003092-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01656"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96644.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="261" mass="27494" checksum="7868DF67C0A5E458" modified="2013-06-26" version="1">MAIAIAIASGKGGTGKTTIAANLAVALAKFGKKVAVLDADIAMANLELIMGLEGKPITLNDVLAGKADIREAIYKGPEGVLVIPAGVSLEKFRRANPEKLKDVLKEIHDLVEILIIDCPAGIGKETLIAISSADGLIVVVNPEISSISDALKLIAITKRLGTEIIGAIVNRVSNESTELSIKAIETILEVKVIGVVPEDPHVRKAAAFGTPLVVMYPDSPAAQAIMEIAAKLIGVEYTPKIKESKKESFITRFLKGLFGGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWJ5</accession>
    <name>N6UWJ5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Cell wall binding repeat 2-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00981</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96699.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWJ5"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="87"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96699.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="87" mass="9521" checksum="9C631BEF313E2E09" modified="2013-06-26" version="1" fragment="single">MVKKLVLIILSLLIPTVAATNVILVSDNQADYLTALNIASLFNDTKVVVTPWGIYNESVVNEILKMKPEQVIIIGGPIAVPDEYVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWQ5</accession>
    <name>N6UWQ5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Monovalent cation/H+ antiporter subunit G</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589760.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWQ5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00702"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.75"/>
    <dbReference type="OrthoDB" id="65729at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004928">
        <property type="entry name" value="PRK06286.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="38"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="88" mass="10101" checksum="632172078BC71593" modified="2013-06-26" version="1">MIELIKDILIFISSLGILIASYRLWIERDRKNIIYARIHILGVIDCACFIIFIALGKILLAIVYLILAPFLAHAIANAYYKDKYKDSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWS0</accession>
    <name>N6UWS0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">High-affinity branched-chain amino acid transport protein BraE</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_00466</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589775.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWS0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00687"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.90"/>
    <dbReference type="OrthoDB" id="15394at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015658">
        <property type="term" value="F:branched-chain amino acid transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06581">
        <property type="entry name" value="TM_PBP1_LivM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043428">
        <property type="entry name" value="LivM-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482:SF1">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT PERMEASE PROTEIN LIVM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30482">
        <property type="entry name" value="HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="87"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="125"/>
            <end position="152"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="220"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="246"/>
            <end position="264"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="271"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="302"/>
            <end position="320"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="341" mass="37718" checksum="CFDA743F353F8F42" modified="2013-06-26" version="1">MDVISIISLILFWFGIYYIVSLSLNIEFGYAGIPNFGKALSVLIGAIAVGGVLDRLLMLYFNVNGDLINGSSIAVSKINYIIAHDPIVGFLILIISIVLAVIIGLLAGAVFILPSAKLKEDYLGVTLLAISETVFLISMFNLDIVGGYYGIATPDILAFISGEYRGLAFTLLTLFIAFLIFLFVERLLNTPFGRILRAMRENEVTVKAFGRNIMLLRIKAMAIGTAIGAIAGVLYVLYSANIVAQAFTRAEWTFFPFLMVLLGGKGNNKGVALGVFCYVVIKIFLDVYKYQIKYLLNIPFEPVWISYILFGMLMLLILYYKPSGLIPEKPILTPPIKKRFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWU9</accession>
    <name>N6UWU9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">glutamine--fructose-6-phosphate transaminase (isomerizing)</fullName>
            <ecNumber evidence="2">2.6.1.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_00180</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>D-fructose 6-phosphate + L-glutamine = D-glucosamine 6-phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:13237"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="ChEBI" id="CHEBI:61527"/>
            <dbReference type="EC" id="2.6.1.16"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.16" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96819.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589725.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWU9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01132"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.36"/>
    <dbReference type="OrthoDB" id="372195at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004360">
        <property type="term" value="F:glutamine-fructose-6-phosphate transaminase (isomerizing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006002">
        <property type="term" value="P:fructose 6-phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006047">
        <property type="term" value="P:UDP-N-acetylglucosamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00081">
        <property type="entry name" value="Hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05008">
        <property type="entry name" value="SIS_GlmS_GlmD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd05009">
        <property type="entry name" value="SIS_GlmS_GlmD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.16.10">
        <property type="entry name" value="Hedgehog/Intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.28.10">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005855">
        <property type="entry name" value="GFAT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035466">
        <property type="entry name" value="GlmS/AgaS_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035490">
        <property type="entry name" value="GlmS/FrlB_SIS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027434">
        <property type="entry name" value="Homing_endonucl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004042">
        <property type="entry name" value="Intein_endonuc_central"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004860">
        <property type="entry name" value="LAGLIDADG_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01135">
        <property type="entry name" value="glmS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001484">
        <property type="entry name" value="PRK00331.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937">
        <property type="entry name" value="GLUCOSAMINE--FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE, ISOMERIZING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10937:SF0">
        <property type="entry name" value="GLUTAMINE--FRUCTOSE-6-PHOSPHATE TRANSAMINASE (ISOMERIZING)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13537">
        <property type="entry name" value="GATase_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14890">
        <property type="entry name" value="Intein_splicing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14528">
        <property type="entry name" value="LAGLIDADG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="Hedgehog/intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55608">
        <property type="entry name" value="Homing endonucleases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50819">
        <property type="entry name" value="INTEIN_ENDONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 12" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="7" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="8" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="10">
        <location>
            <begin position="2"/>
            <end position="552"/>
        </location>
    </feature>
    <feature type="domain" description="DOD-type homing endonuclease" evidence="9">
        <location>
            <begin position="222"/>
            <end position="311"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="11">
        <location>
            <begin position="615"/>
            <end position="752"/>
        </location>
    </feature>
    <feature type="domain" description="SIS" evidence="11">
        <location>
            <begin position="777"/>
            <end position="919"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012916"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96819.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="929" mass="105814" checksum="45D1546D5B6666FC" modified="2013-06-26" version="1">MCGIIGYIGNKKASEVLLNGLRRLEYRGYDSCGIGVIDEDLIIKKDVGKVEEVAKKEDFLSVNGNIGVGHCLHPDTYVVLSDGRIKKISEIDEDEVLSVNFNDFKLYKKKIIKFKHKSPNILYKIKTDFSELLTTGEHKIFVLEEGKIVEKCVKDLNGNELVGVVRKIDYKFDNNEMNPELLQIIGYTIIKKHIKNGTLFLRDREREVLEKYRELFTKIFNINGIIKKEEDYYLLEINSKELINWFKNNVPELFHQNEKTPSFVFKLSNSLLASYLKGVFDSSYVDDKIYLEINSEEFLRETQFLLLRFGILASYSKNGKFKIIIDDKISLESFKKYIGFTSKSKLEKLNKIEGNENLKYFNGNIVWTKFKIEKVESDVEYVYDLEVEDFQNFIGNLIINHNSRWATHGRVCKENAHPHTDCKDEIAVVHNGIISNYRELKDELIKKGHKFKSETDSEIIAHLIEDLIEDNSEEGYIKAVKEAIKRLEGSYAVAIINKRFPNILIGAKNESPLIVGIGEDEAFLGSDITAFLEYTNKAIPLYDGDIVIIKKGENGLDIKIENNGKDVKREVIEINWDINQAEKKGYPHFMLKEIMEQPEILKISSKISSEEIKKLAKLIKDSEKVYFIGMGTSYHACIYAEYLFSKLGKLVIACDASEFLNKGVVDEKTLVIALTQSGETYDTLKAMRYAKERGAKVGAIVNILGSTATREADITVLMGAGLEIAVCATKTFTSQLMIIYRLFIEFGKLLNKDMAVYERAIEKIPKYVKEVLNKKDYIERVTKDLKVNNYIFISRGINIVSALEGALKFKEITYLHAEGMSAGMLKHGTISLIDERMDTIAIVPPKNSRVFNSIISNIEEVKARNGKVIAITPVEIETADYNIITPNVIEEISPIIYAPAYQLLAYYKAVQLNRDVDKPRGLAKSVTVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6UZX2</accession>
    <name>N6UZX2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Protein-glutamate methylesterase/protein-glutamine glutaminase</fullName>
            <ecNumber evidence="3">3.1.1.61</ecNumber>
            <ecNumber evidence="3">3.5.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cheB</name>
        <name evidence="8" type="ORF">J422_07107</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Involved in chemotaxis. Part of a chemotaxis signal transduction system that modulates chemotaxis in response to various stimuli. Catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins or MCP) by CheR. Also mediates the irreversible deamidation of specific glutamine residues to glutamic acid.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-glutaminyl-[protein] + H2O = L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16441"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="EC" id="3.5.1.44"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2 3">
            <text>[protein]-L-glutamate 5-O-methyl ester + H2O = L-glutamyl-[protein] + methanol + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23236"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="Rhea" id="RHEA-COMP:10311"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17790"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:82795"/>
            <dbReference type="EC" id="3.1.1.61"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="3">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="3">Contains a C-terminal catalytic domain, and an N-terminal region which modulates catalytic activity.</text>
    </comment>
    <comment type="PTM">
        <text evidence="3">Phosphorylated by CheA. Phosphorylation of the N-terminal regulatory domain activates the methylesterase activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CheB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.61" evidence="3"/>
    <dbReference type="EC" id="3.5.1.44" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000074">
        <property type="protein sequence ID" value="ENN95573.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594968.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UZX2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01215"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1375"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000156">
        <property type="term" value="F:phosphorelay response regulator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008984">
        <property type="term" value="F:protein-glutamate methylesterase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050568">
        <property type="term" value="F:protein-glutamine glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd16432">
        <property type="entry name" value="CheB_Rec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd17541">
        <property type="entry name" value="REC_CheB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.180">
        <property type="entry name" value="Methylesterase CheB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00099">
        <property type="entry name" value="CheB_chemtxs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008248">
        <property type="entry name" value="CheB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035909">
        <property type="entry name" value="CheB_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000673">
        <property type="entry name" value="Sig_transdc_resp-reg_Me-estase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001965">
        <property type="entry name" value="PRK00742.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42872">
        <property type="entry name" value="PROTEIN-GLUTAMATE METHYLESTERASE/PROTEIN-GLUTAMINE GLUTAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42872:SF3">
        <property type="entry name" value="PROTEIN-GLUTAMATE METHYLESTERASE_PROTEIN-GLUTAMINE GLUTAMINASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01339">
        <property type="entry name" value="CheB_methylest"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000876">
        <property type="entry name" value="RR_chemtxs_CheB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52738">
        <property type="entry name" value="Methylesterase CheB, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50122">
        <property type="entry name" value="CHEB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="3" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 3 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3 5" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Response regulatory" evidence="6">
        <location>
            <begin position="1"/>
            <end position="108"/>
        </location>
    </feature>
    <feature type="domain" description="CheB-type methylesterase" evidence="7">
        <location>
            <begin position="157"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="173"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="199"/>
        </location>
    </feature>
    <feature type="active site" evidence="3 4">
        <location>
            <position position="296"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="3 5">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00048267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95573.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="357" mass="38621" checksum="715A69F37DFE8442" modified="2013-06-26" version="1">MRKVLSDILNSDPEIEVVGTAKDGVEAVEMTLKLKPDVITMDVEMPRMNGLEAVKKIMELCPTPIVMVSALTTEGSRITFEALEAGAVDFIPKPSGSISLDIRKIGDELIRKVKAAAKAKVVKITPKVAKPEVEKKPEAKSEVVSIPKAQEVIEDLPYPDDILKTMCVIIGSSTGGPPVVTEIISKLPKRMPPIFVVQHMPEGFTKLFADRIDRVSKLKAKEAEHGERVQPGYVYVAPGDYHMLLKKRGSEVYIILDNKMPKVNGTRPAVDVTADSVANVYGGRAVGVILTGIGRDGAYGFKKIKEKGGKIIAQNKETCVVFGMPKAVIEMGIADAILPPSEIPPTIVKFCKEILGG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6V029</accession>
    <name>N6V029_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL34</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rpl34e</name>
        <name evidence="6" type="ORF">J422_06658</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL34 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000057">
        <property type="protein sequence ID" value="ENN95648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593898.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V029"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00719"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1296"/>
    <dbReference type="OrthoDB" id="43096at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00349">
        <property type="entry name" value="Ribosomal_eL34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008195">
        <property type="entry name" value="Ribosomal_eL34"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038562">
        <property type="entry name" value="Ribosomal_eL34_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018065">
        <property type="entry name" value="Ribosomal_eL34_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047868">
        <property type="entry name" value="Ribosomal_L34e_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003143">
        <property type="entry name" value="PRK04059.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10759">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L34"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01199">
        <property type="entry name" value="Ribosomal_L34e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01250">
        <property type="entry name" value="RIBOSOMALL34"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01145">
        <property type="entry name" value="RIBOSOMAL_L34E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="89" mass="10526" checksum="3DAC8B8D21E6A614" modified="2013-06-26" version="1">MPAPRYRSRSYRRVYKRTPGGRLVIHYKRKKPNKPKCALCGAELHGVPRGRPVEIRKLPKSQRRPERPYGGYLCPKCLRRLMIEKARKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6V032</accession>
    <name>N6V032_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UPF0273 protein J422_06683</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_06683</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0273 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000057">
        <property type="protein sequence ID" value="ENN95653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593908.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V032"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00714"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1301"/>
    <dbReference type="OrthoDB" id="27015at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd19486">
        <property type="entry name" value="KaiC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01076">
        <property type="entry name" value="UPF0273"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014774">
        <property type="entry name" value="KaiC-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010624">
        <property type="entry name" value="KaiC_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022475">
        <property type="entry name" value="UPF0273_KaiC-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03877">
        <property type="entry name" value="thermo_KaiC_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43637:SF1">
        <property type="entry name" value="UPF0273 PROTEIN TM_0370"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06745">
        <property type="entry name" value="ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01874">
        <property type="entry name" value="DNAREPAIRADA"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51146">
        <property type="entry name" value="KAIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="KaiC" evidence="4">
        <location>
            <begin position="2"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="29"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01076"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="242" mass="26972" checksum="EE3DA7967C27E8E8" modified="2013-06-26" version="1">MKRVKTGIPGMDEILHGGIPERNVVLLSGGPGTGKSIFCQQFLYKGVTEFNEPAVLIALEEHPVQVRENMKQFGWDVKKLEEEGKFAIIDAFTYGIGAAAKRERYVVNDPTDERELIDVLKQAINDISAKRIGIDSVTTLYINKPMMARRTVFLLKRVIAGLGCTAIFTSQISVGERGFGGPGVEHAVDGIIRLDLDEVDGELKRSLIVWKMRGTSHSLRRHPFEITDNGIIVYHDKVLKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6V056</accession>
    <name>N6V056_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Thymidylate synthase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06321</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593500.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V056"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1230"/>
    <dbReference type="OrthoDB" id="146618at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003745">
        <property type="entry name" value="DUF166"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02593">
        <property type="entry name" value="DUF166"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="208" mass="24411" checksum="97371C454397C571" modified="2013-06-26" version="1">MKVAILSEGKYGYRAYKNINRIFNCDFLKISYVGDLDNIIIDENLLNSLKHDIYIIYANQDVTYELIRNIKDGFVFVGIWRGDGFKKQVEKFDNVYSPRFLCEIDEEVLKDKLNKYPQLKEFLKYFGKPKVNLYVKDNKIVDIKIFRRSICGSTEAIYEFLNKEPNLKNIGLRVQHFCVAGKPNVFKNFCPKSEIAKILIDNIRVIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="55" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0F8</accession>
    <name>N6V0F8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1 6">Shikimate dehydrogenase (NADP(+))</fullName>
            <shortName evidence="6">SDH</shortName>
            <ecNumber evidence="1 6">1.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6 10" type="primary">aroE</name>
        <name evidence="10" type="ORF">J422_05698</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the biosynthesis of the chorismate, which leads to the biosynthesis of aromatic amino acids. Catalyzes the reversible NADPH linked reduction of 3-dehydroshikimate (DHSA) to yield shikimate (SA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>shikimate + NADP(+) = 3-dehydroshikimate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17737"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16630"/>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="EC" id="1.1.1.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the shikimate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.25" evidence="1 6"/>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95808.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592793.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0F8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01263"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1111"/>
    <dbReference type="OrthoDB" id="8744at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00087"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004764">
        <property type="term" value="F:shikimate 3-dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019632">
        <property type="term" value="P:shikimate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01065">
        <property type="entry name" value="NAD_bind_Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000086">
        <property type="entry name" value="Quinate/shikimate dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10860">
        <property type="entry name" value="Leucine Dehydrogenase, chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00222">
        <property type="entry name" value="Shikimate_DH_AroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046346">
        <property type="entry name" value="Aminoacid_DH-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041121">
        <property type="entry name" value="SDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011342">
        <property type="entry name" value="Shikimate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013708">
        <property type="entry name" value="Shikimate_DH-bd_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022893">
        <property type="entry name" value="Shikimate_DH_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006151">
        <property type="entry name" value="Shikm_DH/Glu-tRNA_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00507">
        <property type="entry name" value="aroE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001319">
        <property type="entry name" value="PRK00258.3-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089:SF1">
        <property type="entry name" value="BIFUNCTIONAL 3-DEHYDROQUINATE DEHYDRATASE_SHIKIMATE DEHYDROGENASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21089">
        <property type="entry name" value="SHIKIMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18317">
        <property type="entry name" value="SDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01488">
        <property type="entry name" value="Shikimate_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08501">
        <property type="entry name" value="Shikimate_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53223">
        <property type="entry name" value="Aminoacid dehydrogenase-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="3 6" id="KW-0521">NADP</keyword>
    <keyword evidence="4 6 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Shikimate dehydrogenase substrate binding N-terminal" evidence="8">
        <location>
            <begin position="11"/>
            <end position="93"/>
        </location>
    </feature>
    <feature type="domain" description="Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase" evidence="7">
        <location>
            <begin position="114"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="SDH C-terminal" evidence="9">
        <location>
            <begin position="242"/>
            <end position="272"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="19"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="129"/>
            <end position="133"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="151"/>
            <end position="156"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="242"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="249"/>
        </location>
        <ligand>
            <name>shikimate</name>
            <dbReference type="ChEBI" id="CHEBI:36208"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01488"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF08501"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF18317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95808.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="279" mass="30674" checksum="A3F391ECC6C3138E" modified="2013-06-26" version="1">MIDSKTKLVALIGHPVEHSLSPIMQNAAFRDKNLNYVYLAFDVLPEKLKYVIDGAKALGIVGFNVTIPHKIEIIKYLDKLDKSAELIGAVNTIKVDKEAIGYNTDGIGARKALEEKIGKVENKNILIFGAGGAARAVAFELAKNNNIIIANRTLDKAKKLAEEIIEKLGKNSDEVKYVPLNTDLKDVDIVINATSVGMKKDDKPLATAEQLRGKVVMDLVYNPLETPLLKEAKKVNAIAIDGLGMLIHQGAEAFKIWTGVEPNINVMREAIMKYLNYSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0N8</accession>
    <name>N6V0N8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05444</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000040">
        <property type="protein sequence ID" value="ENN95878.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592510.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000040.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0N8"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1063"/>
    <dbReference type="OrthoDB" id="60268at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95878.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="174" mass="20436" checksum="619384952B38AAA8" modified="2013-06-26" version="1">MEYLPKEIVSTIYRKAILHYMLITGSTFPQKLSEDLNISKGLACSFLRLCSALGFMERKRLGHRVYYNLTKVGIAKLKRMAAEIFDLSFSNVFEILSKKKFTLKHYPLSKVGFSIKWEKDFLGGYTFYFYDSNGELIGKVFRNTFGEWWCVPCQSKNCKHIEYLKKLYEKIKDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0V5</accession>
    <name>N6V0V5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">precorrin-2 dehydrogenase</fullName>
            <ecNumber evidence="2">1.3.1.76</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_04830</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>precorrin-2 + NAD(+) = sirohydrochlorin + NADH + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:15613"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:58827"/>
            <dbReference type="EC" id="1.3.1.76"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; siroheme biosynthesis; sirohydrochlorin from precorrin-2: step 1/1.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.76" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95938.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592048.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0V5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00524"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.946"/>
    <dbReference type="OrthoDB" id="10510at2157"/>
    <dbReference type="UniPathway" id="UPA00262">
        <property type="reaction ID" value="UER00222"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004325">
        <property type="term" value="F:ferrochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043115">
        <property type="term" value="F:precorrin-2 dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019354">
        <property type="term" value="P:siroheme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.610">
        <property type="entry name" value="SirC, precorrin-2 dehydrogenase, C-terminal helical domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028161">
        <property type="entry name" value="Met8-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042518">
        <property type="entry name" value="SirC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006367">
        <property type="entry name" value="Sirohaem_synthase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01470">
        <property type="entry name" value="cysG_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35330:SF1">
        <property type="entry name" value="SIROHEME BIOSYNTHESIS PROTEIN MET8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13241">
        <property type="entry name" value="NAD_binding_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75615">
        <property type="entry name" value="Siroheme synthase middle domains-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95938.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="207" mass="24020" checksum="768A4D25F7AC6703" modified="2013-06-26" version="1">MLPILLSFENKKVAVFGCGEVGKRRALKILKNGGNVDIYSKNFDNEIKKIKNDRLKLINVDLNKLSDEELREIIKDYDFIVAAVNEEINRRIVKIANELNKFVNASNLMSGVNFILPAYTEVNGVIFSIYTRGKSPMLAKKIRILVENIIRDQDISFLSEFREFLKEIVPNQKDRKKVIEELFNNKEFREELKKIVMRVLNDHSSKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6V105</accession>
    <name>N6V105_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Group 1 glycosyl transferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_05030</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95978.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592129.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V105"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00483"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.986"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="2">
        <location>
            <begin position="13"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="167"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95978.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="336" mass="38669" checksum="E621A0F802C33AFB" modified="2013-06-26" version="1">MKVLMPTIFYPHIGGITVHVENLVKNLEDIEFYLITYDYSKPKYKNIHIYNVPYLKRFRGVSYLINAYRVSKKIIDKEKIDIIHSHYAFPQGCLGGLLKKRYGIPHILTLHGSDALILKNSLLGKKFFNFAIENADEIICVSNYIKETVGRGRVIYNGVDREMLYNEGDHNFALFVGSFVKQKGVDILIKEIEDIDYNFILIGDGPLYKEVYNYISKKGLGHIMLLGKKKFNEVAYYMRKCSFLIVPSRSEGFGLTAIEAMACGKPVVARNVGGLREIVINNYNGFLTNNLKEKIELLINDKNLRETLGKNAYKFSKKFSWEKTAEEVRKVYEEIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1A2</accession>
    <name>N6V1A2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04258</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96053.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1A2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00671"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.833"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005266">
        <property type="entry name" value="UPF0128"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00703">
        <property type="entry name" value="TIGR00703 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03673">
        <property type="entry name" value="UPF0128"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96053.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="210" mass="24161" checksum="16E9BF52345CF801" modified="2013-06-26" version="1">MVLEPKELEVVNTLVFETLGNPEREREFKLKTLKKWGFDLIFGKINGKETYFTAEIDTKKAGDKYTIDEKEYEVIEVLDELPKNTELYAHIEMEMGMAYIVCQLRDEDSKNTEILRVPAGSLLMAFFKKNKLGNLIKAIKNIGISSEFFMQNGAGGEPYSYENLPNIARRFIRSVRKVEKETGFGRLAFAYYGETKDGEPDIGYLGYYLQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1J5</accession>
    <name>N6V1J5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Xanthine/uracil/vitamin C permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_03778</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the nucleobase:cation symporter-2 (NCS2) (TC 2.A.40) family. Azg-like subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96148.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591345.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1J5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00388"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.740"/>
    <dbReference type="OrthoDB" id="27788at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005345">
        <property type="term" value="F:purine nucleobase transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045018">
        <property type="entry name" value="Azg-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026033">
        <property type="entry name" value="Azg-like_bact_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006043">
        <property type="entry name" value="NCS2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337">
        <property type="entry name" value="XANTHINE/URACIL PERMEASE C887.17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43337:SF1">
        <property type="entry name" value="XANTHINE_URACIL PERMEASE C887.17-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00860">
        <property type="entry name" value="Xan_ur_permease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005353">
        <property type="entry name" value="PbuG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="21"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="49"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="77"/>
            <end position="97"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="133"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="169"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="195"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="235"/>
            <end position="253"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="319"/>
            <end position="337"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="343"/>
            <end position="363"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="375"/>
            <end position="404"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="416"/>
            <end position="433"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR005353"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96148.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="434" mass="46542" checksum="00FD85D4C6659A35" modified="2013-06-26" version="1">MKIIEDYFEFKKYGTNIKIEILAGITTFMTMAYIIFVNPQILSQTGMDFGAVMVATCIASAIATLIMGLYAKYPFALAPGMGLNAYFTYGVCLGMGIDWRVALGAVFISGILFIILTLTKVRTWIFNVIPNAIKYGTAVGIGLFIAFIGLKNAGIIVESKATLVTLGNILNPEPLLSLFGIFLTSILVSRNVIGGILIGIIITSLIGMIFGISQFPSGIVSLPPSIEPTFLKLDIFGALNLGLLTIVMSFFFVDMFDTIGTLSALSSQAGYLDKDGKLPRVEKALMSDATGTVLGSLLGTSTVTTYIESASGIAMGGKTGFVSVVVALLFLLSLFFYPLVKAIPTYATASALVIVGALMMKAIKNINFDDYTESIPAFITILTIPLTFSIATGLALGFITYPILKIFTGRAKEVHWLVYILAAIFALRFVYLSG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1L1</accession>
    <name>N6V1L1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Probable manganese-dependent inorganic pyrophosphatase</fullName>
            <ecNumber evidence="8">3.6.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pyrophosphate phospho-hydrolase</fullName>
            <shortName evidence="8">PPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">ppaC</name>
        <name evidence="10" type="ORF">J422_03898</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>diphosphate + H2O = 2 phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24576"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="EC" id="3.6.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="8">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the PPase class C family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.1" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96168.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591383.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1L1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00410"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.764"/>
    <dbReference type="OrthoDB" id="114945at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004427">
        <property type="term" value="F:inorganic diphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.310.20:FF:000001">
        <property type="entry name" value="Probable manganese-dependent inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.1640.10:FF:000001">
        <property type="entry name" value="Probable manganese-dependent inorganic pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.20">
        <property type="entry name" value="DHHA2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00207">
        <property type="entry name" value="PPase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004097">
        <property type="entry name" value="DHHA2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038222">
        <property type="entry name" value="DHHA2_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022934">
        <property type="entry name" value="Mn-dep_inorganic_PyrPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003877">
        <property type="entry name" value="PRK05427.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112">
        <property type="entry name" value="BNIP - RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12112:SF22">
        <property type="entry name" value="MANGANESE-DEPENDENT INORGANIC PYROPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02833">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01131">
        <property type="entry name" value="DHHA2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DHHA2" evidence="9">
        <location>
            <begin position="180"/>
            <end position="305"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="11"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047820"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00207"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96168.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="307" mass="34359" checksum="C5FA4EF51B28A348" modified="2013-06-26" version="1">MRYVVGHKNPDTDSIASAIVLAYFLDCYPARLGNLNPETEFVLRKFGIMEPELIESAKGKELILVDHSEKSQSLDDLDEGKLLAIIDHHKVGLTTVEPILYYAKPVGSTATIIAELYFKKCLDLIGGKNKELKPDLAGLLLSAIISDTVLFKSPTTTELDKEMAKKLAEIAGIEDIEKFGLEILKAKSSVSKLSANEIINMDFKDYEFNGKKVGVGQVEVIDISEIEDKKEEIYKLLKEKLEKEGYDLIVFMITDIMKEGSEMLIIGNKEMFEKAFNIKLDGDSIFLEGVMSRKKQVIPPLERAYKG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1M7</accession>
    <name>N6V1M7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methanogenesis marker domain 9</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03528</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96188.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591229.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1M7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01397"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.688"/>
    <dbReference type="OrthoDB" id="145053at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017671">
        <property type="entry name" value="Methan_mark_9"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03277">
        <property type="entry name" value="methan_mark_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96188.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="151" mass="17194" checksum="A3F7301B00A58AFE" modified="2013-06-26" version="1">MWKNAPSHICRGGDLRGIAFCCPPVKPCPLLKALKILKLSPEEYVRIKEEFAKKTKLGLGENTCFGSLVWCCKITKPCPLRDYELRRNNISPEEYMMLKKLLAEEILKNSPLIKEAIELFVKKGIPRDIAEKCLLETGDIKKAYEKAKTIV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1N1</accession>
    <name>N6V1N1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Rubredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_03553</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Rubredoxin is a small nonheme, iron protein lacking acid-labile sulfide. Its single Fe, chelated to 4 Cys, functions as an electron acceptor and may also stabilize the conformation of the molecule.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 8">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
        <text evidence="7 8">Binds 1 Fe(3+) ion per subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the rubredoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96193.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591238.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1N1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01392"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.693"/>
    <dbReference type="OrthoDB" id="371635at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043448">
        <property type="term" value="P:alkane catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00730">
        <property type="entry name" value="rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000001">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024922">
        <property type="entry name" value="Rubredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024935">
        <property type="entry name" value="Rubredoxin_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050526">
        <property type="entry name" value="Rubredoxin_ET"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47627">
        <property type="entry name" value="RUBREDOXIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47627:SF1">
        <property type="entry name" value="RUBREDOXIN-1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00301">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000071">
        <property type="entry name" value="Rubredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00163">
        <property type="entry name" value="RUBREDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 7 8" id="KW-0408">Iron</keyword>
    <keyword evidence="4 7 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="9">
        <location>
            <begin position="1"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002360"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005337"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR000071"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000071-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96193.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="52" mass="5917" checksum="87A9C65B1B833390" modified="2013-06-26" version="1">MAKYQCMCGWIYDEDKGEPSQNIPPGTKFEDLPDDFRCPQCGLGKNAFTKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1Q2</accession>
    <name>N6V1Q2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Aspartokinase</fullName>
            <ecNumber evidence="13">2.7.2.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">J422_03708</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13">
            <text>L-aspartate + ATP = 4-phospho-L-aspartate + ADP</text>
            <dbReference type="Rhea" id="RHEA:23776"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57535"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 14">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 1/4.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 14">Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3.</text>
    </comment>
    <comment type="pathway">
        <text evidence="3 14">Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 13">Belongs to the aspartokinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.4" evidence="13"/>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96218.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591285.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1Q2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01364"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.724"/>
    <dbReference type="OrthoDB" id="8904at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00015"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00050">
        <property type="reaction ID" value="UER00461"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00051">
        <property type="reaction ID" value="UER00462"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004072">
        <property type="term" value="F:aspartate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009090">
        <property type="term" value="P:homoserine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009088">
        <property type="term" value="P:threonine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd04892">
        <property type="entry name" value="ACT_AK-like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04921">
        <property type="entry name" value="ACT_AKi-HSDH-ThrA-like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000017">
        <property type="entry name" value="Bifunctional aspartokinase/homoserine dehydrogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.10">
        <property type="entry name" value="VC0802-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054352">
        <property type="entry name" value="ACT_Aspartokinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005260">
        <property type="entry name" value="Asp_kin_monofn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001341">
        <property type="entry name" value="Asp_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018042">
        <property type="entry name" value="Aspartate_kinase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00656">
        <property type="entry name" value="asp_kin_monofn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00657">
        <property type="entry name" value="asp_kinases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004938">
        <property type="entry name" value="PRK06291.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499">
        <property type="entry name" value="ASPARTATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21499:SF59">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22468">
        <property type="entry name" value="ACT_9"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000726">
        <property type="entry name" value="Asp_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00324">
        <property type="entry name" value="ASPARTOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 14" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10 13 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0677">Repeat</keyword>
    <keyword evidence="7" id="KW-0791">Threonine biosynthesis</keyword>
    <keyword evidence="6 13 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ACT" evidence="15">
        <location>
            <begin position="406"/>
            <end position="468"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004986"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005139"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022697"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00047872"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN96218.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="468" mass="50909" checksum="AF8464B5A67A5D7E" modified="2013-06-26" version="1">MVVVMKFGGTSVGDGKKIRKVAEIVRDRKKDDKVVVVVSAMSEVTNALIDISQQALDVRDLEKISDFISFIKDKHYSAIEDAIKDENIKKHVKKIIESRIEELEKVLIGVAYLGELTKRSKDYILSFGERLSAPILAGALNDLGEKSIALEGGEAGIITDNNFGNARVVKLKVKEMLTPLIERDIIPVVTGFIGLSEDGYITTLGRGGSDYSAALIGYGLDASIIEIWTDVSGVYTADPKLVPNARRIPKLSYIEAMELAYFGAKVLHPRTIEPAMEKHIPILVKNTFKPEDEGTLITNNIEYSDRVVKAITTIKDVALINIFGAGMVGVSGTAARIFKALEDVNVILISQGSSETNISIVVSKEDADRAVKSLKNEFGVFYKKSFLNNSVIRGVDVDKDVSVVSVVGAGMRGAKGIAGKIFTTVAESGANIKMIAQGSSEVNISFVIDKKDLINCVRKLHEKFIEAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6V200</accession>
    <name>N6V200_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">sulfopyruvate decarboxylase</fullName>
            <ecNumber evidence="7">4.1.1.79</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_03099</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the biosynthesis of the coenzyme M (2-mercaptoethanesulfonic acid). Catalyzes the decarboxylation of sulfopyruvate to sulfoacetaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>3-sulfopyruvate + H(+) = sulfoacetaldehyde + CO2</text>
            <dbReference type="Rhea" id="RHEA:20948"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57940"/>
            <dbReference type="ChEBI" id="CHEBI:58246"/>
            <dbReference type="EC" id="4.1.1.79"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; coenzyme M biosynthesis; sulfoacetaldehyde from phosphoenolpyruvate and sulfite: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterododecamer composed of 6 subunits alpha and 6 subunits beta.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.79" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96313.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590768.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V200"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00166"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.608"/>
    <dbReference type="OrthoDB" id="77140at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050545">
        <property type="term" value="F:sulfopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019295">
        <property type="term" value="P:coenzyme M biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03372">
        <property type="entry name" value="TPP_ComE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022494">
        <property type="entry name" value="Sulfopyruvate_deCO2ase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051818">
        <property type="entry name" value="TPP_dependent_decarboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03846">
        <property type="entry name" value="sulfopy_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42818:SF1">
        <property type="entry name" value="SULFOPYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42818">
        <property type="entry name" value="SULFOPYRUVATE DECARBOXYLASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0174">Coenzyme M biosynthesis</keyword>
    <keyword evidence="2" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="9">
        <location>
            <begin position="39"/>
            <end position="154"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96313.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="184" mass="20391" checksum="113DD15DFBA136B6" modified="2013-06-26" version="1">MKRIDVIKKIVDNVDKELIVCNIGIPSKELYSVKDRERNFYMLGSMGLASSIGLGLALNIKEKVVVIDGDGSILMNLGSLSTIGYLKPKNLILVIIDNSSYGSTGNQKTHTAKNTNLKRVAEACGLCAVEVDNIDNFEEVFKCALKRDETFVIIAKTIPYNEPCEDINIPPVVIKYRFMKAIHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6V273</accession>
    <name>N6V273_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02849</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0166 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96378.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590636.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V273"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00119"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.559"/>
    <dbReference type="OrthoDB" id="8505at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015867">
        <property type="entry name" value="N-reg_PII/ATP_PRibTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003793">
        <property type="entry name" value="UPF0166"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35983">
        <property type="entry name" value="UPF0166 PROTEIN TM_0021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35983:SF1">
        <property type="entry name" value="UPF0166 PROTEIN TM_0021"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02641">
        <property type="entry name" value="DUF190"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96378.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="107" mass="12196" checksum="36452CB66C465179" modified="2013-06-26" version="1">MLRAKILRIYLKEGDKYNDEPAYKYILKLLKKEGISGATVFRGVAGYGVRGISQMDIFRLSINLPIVIECVDLEENINRVLPKIHKVINNNGLIVVMKCKVYLGEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2A0</accession>
    <name>N6V2A0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">HTH arsR-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_02424</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96398.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590427.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2A0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00786"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.474"/>
    <dbReference type="OrthoDB" id="85881at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.450">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052362">
        <property type="entry name" value="HTH-GbsR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026282">
        <property type="entry name" value="MJ1563"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38465">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR MJ1563-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38465:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR MJ1563-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006707">
        <property type="entry name" value="MJ1563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="4">
        <location>
            <begin position="31"/>
            <end position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96398.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="144" mass="16702" checksum="34F387990EDE30D0" modified="2013-06-26" version="1">MEDYEKKIIEMFSEISKLHGLSKSIGAIYAVLFLSDEPLSIPDIVEKLKISKGNVSMGLKKLEELGFIRKVMVIGKRRNYYVVEDRFSSIKDIAKKKYEIISKTYESVKDSKLPKDKLKKLEKMKNLSEKILKVLEEINNEENI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2H9</accession>
    <name>N6V2H9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Hydrogenase nickel incorporation protein HypB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_02225</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIMIBI class G3E GTPase family. HypB/HupM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96468.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590272.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2H9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01193"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.437"/>
    <dbReference type="OrthoDB" id="812at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003495">
        <property type="entry name" value="CobW/HypB/UreG_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004392">
        <property type="entry name" value="Hyd_mat_HypB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00073">
        <property type="entry name" value="hypB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30134:SF2">
        <property type="entry name" value="HYDROGENASE MATURATION FACTOR HYPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30134">
        <property type="entry name" value="HYDROGENASE PROTEIN ASSEMBLY PROTEIN, NICKEL CHAPERONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02492">
        <property type="entry name" value="cobW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005624">
        <property type="entry name" value="Ni-bind_GTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0533">Nickel</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="CobW/HypB/UreG nucleotide-binding" evidence="8">
        <location>
            <begin position="38"/>
            <end position="196"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96468.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="218" mass="24198" checksum="73C701C17E51A7AE" modified="2013-06-26" version="1">MHKIGTLEIAKDILKANKRLADENRKLLKKHGIVAFDISGAIGSGKTLLIEKLIEKLKDNYKIACIAGDVIAKYDAERLERHNVKVIPINTGKECHLDAHLIKHALEDLDLNDIDILFIENVGNLICPADFDLGADKRIVVVSTTEGDDIIAKHPEIMRTADLIIINKVDLAKYVGADVNKMIEDAKKINPNAKVITLSLKTMEGFDEVVNYILSNLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2S4</accession>
    <name>N6V2S4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Magnesium transport protein CorA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">corA</name>
        <name evidence="13" type="ORF">J422_02000</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Mediates influx of magnesium ions. Alternates between open and closed states. Activated by low cytoplasmic Mg(2+) levels. Inactive when cytoplasmic Mg(2+) levels are high.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>Mg(2+)(in) = Mg(2+)(out)</text>
            <dbReference type="Rhea" id="RHEA:29827"/>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="12">Membrane</location>
            <topology evidence="12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the CorA metal ion transporter (MIT) (TC 1.A.35) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96543.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590168.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2S4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00245"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.392"/>
    <dbReference type="OrthoDB" id="28779at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015095">
        <property type="term" value="F:magnesium ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd12822">
        <property type="entry name" value="TmCorA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.340:FF:000004">
        <property type="entry name" value="Magnesium transport protein CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.20">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.340">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047199">
        <property type="entry name" value="CorA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045861">
        <property type="entry name" value="CorA_cytoplasmic_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045863">
        <property type="entry name" value="CorA_TM1_TM2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004488">
        <property type="entry name" value="Mg/Co-transport_prot_CorA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002523">
        <property type="entry name" value="MgTranspt_CorA/ZnTranspt_ZntB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00383">
        <property type="entry name" value="corA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47891:SF2">
        <property type="entry name" value="MAGNESIUM AND COBALT TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47891">
        <property type="entry name" value="TRANSPORTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01544">
        <property type="entry name" value="CorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143865">
        <property type="entry name" value="CorA soluble domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144083">
        <property type="entry name" value="Magnesium transport protein CorA, transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8 12" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="256"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="288"/>
            <end position="308"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00034269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00045497"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU362010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96543.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="314" mass="37166" checksum="F98975C46CA99F26" modified="2013-06-26" version="1">MISIVGYKDKVVELKLDDDFKDYKIVWIDCYNPKDDELYKLSKKVNIPINDLKVGLDEQEFPRLEEEEDYYLIIYKAPLYEEDIITTSLGIFIKGNIILTMHTDKIKAIGRLFNIVITKKPKIIFEKGIGFLLYNILNEITRGYSRIILGLEDELEKLEDYLTTGYNREMIEEILSLRKTLVYFHKSLMANRDVLIQLKRRLLPITTKEDRENFEDLYYDTLQLIDTLSTYREVLTSMMDISLSLENMKMNQIMKILTMVTTIFAIPMWITGIYGMNFEYLPLSDNPYGFWLVLTLMIMLIMLFTYIFKRAGWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6V322</accession>
    <name>N6V322_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01076</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96643.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589883.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V322"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00827"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.210"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="17"/>
            <end position="58"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96643.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="59" mass="6876" checksum="81A948E95F34B5D7" modified="2013-06-26" version="1">MDLIVLYVVVFISIILNIALGIKVIELQKELNELKQATRLTKEEVDKLNERLRRLKLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6V330</accession>
    <name>N6V330_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">2-amino-5-formylamino-6-ribosylaminopyrimidin-4(3H)-one 5'-monophosphate deformylase</fullName>
            <shortName evidence="6">FAPy deformylase</shortName>
            <ecNumber evidence="6">3.5.1.102</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Formamide hydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">arfB</name>
        <name evidence="7" type="ORF">J422_01126</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the hydrolysis of the formamide of 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-monophosphate (FAPy) to form 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate (APy).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>2-amino-5-formylamino-6-(5-phospho-D-ribosylamino)pyrimidin-4(3H)-one + H2O = 2,5-diamino-6-(1-D-ribosylamino)pyrimidin-4(3H)-one 5'-phosphate + formate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27282"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:57258"/>
            <dbReference type="ChEBI" id="CHEBI:59545"/>
            <dbReference type="EC" id="3.5.1.102"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Requires an additional second metal ion that could be Fe(2+) or Zn(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="6">Requires one Fe(2+) ion for activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; riboflavin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the creatininase superfamily. FAPy deformylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.102" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96653.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589893.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V330"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00817"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.220"/>
    <dbReference type="OrthoDB" id="46121at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="UniPathway" id="UPA00275"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043729">
        <property type="term" value="F:2-amino-5-formylamino-6-(5-phosphoribosylamino)pyrimidin-4(3H)-one formate-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10310">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02116">
        <property type="entry name" value="FAPy_deform"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024087">
        <property type="entry name" value="Creatininase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003785">
        <property type="entry name" value="Creatininase/forma_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024901">
        <property type="entry name" value="FAPy_deformylase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033501">
        <property type="entry name" value="ArfB_arch_rifla"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005:SF1">
        <property type="entry name" value="2-AMINO-5-FORMYLAMINO-6-RIBOSYLAMINOPYRIMIDIN-4(3H)-ONE 5'-MONOPHOSPHATE DEFORMYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35005">
        <property type="entry name" value="3-DEHYDRO-SCYLLO-INOSOSE HYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02633">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102215">
        <property type="entry name" value="Creatininase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 6" id="KW-0408">Iron</keyword>
    <keyword evidence="2 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="29"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="31"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="40"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96653.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="222" mass="24929" checksum="B8009C73A7C4349E" modified="2013-06-26" version="1">MQKLRLNSGKVLNERVHKVGILAMGSFLENHGAVLPIDTDIKIASYIALKASILTGAKFLGVVIPSTEFSYVKHGIHNKPEDVVNYLKFIINWGKKIGIEEFIIVNCHGGNVIINDYIKDLEKELKVKIWKIDFFFTHAATEEVSIGYVIGIVEGSIEEHNKFNKYPEVGMVGLEEARKMNKDIDEEAKKVEKNGVIIDENLGKKLLDKAIEKVVNKIKSLV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP35</accession>
    <name>N6VP35_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Sodium/hydrogen exchanger</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_06952</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000065">
        <property type="protein sequence ID" value="ENN95605.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594635.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP35"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00857"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1347"/>
    <dbReference type="OrthoDB" id="12029at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006814">
        <property type="term" value="P:sodium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1530.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038770">
        <property type="entry name" value="Na+/solute_symporter_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562">
        <property type="entry name" value="NAPA-TYPE SODIUM/HYDROGEN ANTIPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43562:SF3">
        <property type="entry name" value="SODIUM ION_PROTON EXCHANGER (EUROFUNG)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0915">Sodium</keyword>
    <keyword evidence="9" id="KW-0739">Sodium transport</keyword>
    <keyword evidence="4 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="6"/>
            <end position="21"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="28"/>
            <end position="47"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="53"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="84"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="114"/>
            <end position="132"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="144"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="212"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="263"/>
            <end position="286"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="293"/>
            <end position="312"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="324"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="355"/>
            <end position="373"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="11">
        <location>
            <begin position="13"/>
            <end position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95605.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="380" mass="42173" checksum="78D68341991CC162" modified="2013-06-26" version="1">MDFYYIFFIVLSIIFIIPNLLKRFNIPAITSIMIAGMIIGDYGLKIIEVNETLKVLSELGAIMLMFLAGLEVDNETLKKEFKNSLFLTLLSILIPGIGGYYIGNLIGLNFSGSLLMAVIFASHSVAIVYAVLNELNLTNNRLGIIILSSTIIIDLITLLLLSVIIKINNGDGNLLIFFLKIFVYISILLISIPIISKYILNIFEKLHAQRIHYVLFIIFIVIIIGEYLNIHPIVGAFICGVAVSEALTKKECDEILNKNLNAIGYGFFIPIFFLVLGMETNFWVIFNLQNLKQILLILSSAVLLKVVSSYIPLKLLRFNNKECLFGSLLTVPKISASLVAATIGKEMGIIDNSIFVAIIAMSVITSTLVPIIAKNLKSSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP83</accession>
    <name>N6VP83_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06518</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000053">
        <property type="protein sequence ID" value="ENN95680.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593622.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000053.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP83"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00997"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1268"/>
    <dbReference type="OrthoDB" id="65677at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008713">
        <property type="term" value="F:ADP-heptose-lipopolysaccharide heptosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd03789">
        <property type="entry name" value="GT9_LPS_heptosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002201">
        <property type="entry name" value="Glyco_trans_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051199">
        <property type="entry name" value="LPS_LOS_Heptosyltrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30160:SF1">
        <property type="entry name" value="LIPOPOLYSACCHARIDE 1,2-N-ACETYLGLUCOSAMINETRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30160">
        <property type="entry name" value="TETRAACYLDISACCHARIDE 4'-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01075">
        <property type="entry name" value="Glyco_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95680.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="351" mass="41182" checksum="2D15E7EC632E826A" modified="2013-06-26" version="1">MKILIIALSGIGNLIMAFPMINILKKNYPNSKIDFLVALRGTREVLENQPFVNKIFVLKNQSIRSLLLDNYTKAIIKLLKTNNYDIAITIYPSQGIFSALLMKLIKAKIRIQHKYNFKIFENIDWFLTYSPKIENKHSVYINLDLIKYLNVKYKDEDIRYSYYLTQKEIKFANKFLDKQNLKDEFIIGIHPGGKSDMVWKRWGIKKWKKLIDLLNSEYKNKIKFLIFLGPDEIIYEKYFRNHDNVIIIKNIPLKYVISLISKCNYFISNDSGLSHCASLFNIPQSVIFGGTSYIHTAPFSNKVNIITPPNYEIYYIPYYGFIKKPKNLLMNLEPEDAFESIKTHIKTLNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPA0</accession>
    <name>N6VPA0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glutamate synthase subunit alpha</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06416</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593533.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPA0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01291"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1249"/>
    <dbReference type="OrthoDB" id="2513at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00981">
        <property type="entry name" value="arch_gltB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="entry name" value="Glutamate synthase, alpha subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035710">
        <property type="entry name" value="Archaeal_gltB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002489">
        <property type="entry name" value="Glu_synth_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012061">
        <property type="entry name" value="Glu_synth_lsu_3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39673:SF8">
        <property type="entry name" value="GLUTAMATE SYNTHASE ALPHA SUBUNIT C-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39673">
        <property type="entry name" value="TUNGSTEN FORMYLMETHANOFURAN DEHYDROGENASE, SUBUNIT C (FWDC)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01493">
        <property type="entry name" value="GXGXG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006519">
        <property type="entry name" value="GOGAT_dom3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="Alpha subunit of glutamate synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glutamate synthase alpha subunit C-terminal" evidence="1">
        <location>
            <begin position="14"/>
            <end position="193"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01493"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="257" mass="28559" checksum="680F4183858AC504" modified="2013-06-26" version="1">MEIEIDAKGMHYRELNEKIRKIIKENPDIKKIILKNVLGQRFIGNSLRKKDLTIEIYGTPGGDLGMFMSGPTIIVHGNCEFAPGNTMDDGLIVIHGDSGDVTAHSMRGGKVFVKGDVGYRSGIHMKAYKDKVPILVIGGRAKDFLGEYMAGGKIIVLNIDKEGNDLGKVKGRMIGTGIHGGAIYIRGDVEKDQLGVAADIKEFTEEDLKEIEPYIEEFCKHFNFSEDVKEMLINSKWTKIAPISKRPFGKLYTPDLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPF3</accession>
    <name>N6VPF3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Peptide chain release factor subunit 1</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Translation termination factor aRF1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_06185</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterodimer of two subunits, one of which binds GTP.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic release factor 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593273.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPF3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00556"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1205"/>
    <dbReference type="OrthoDB" id="1011at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.30:FF:000032">
        <property type="entry name" value="Eukaryotic peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.420.60:FF:000003">
        <property type="entry name" value="Peptide chain release factor subunit 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03676">
        <property type="entry name" value="aRF1_eRF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1" evidence="9">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="domain" description="eRF1" evidence="10">
        <location>
            <begin position="128"/>
            <end position="270"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002832"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00019723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031168"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF03465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="270" mass="30475" checksum="FC560EF82AD840EC" modified="2013-06-26" version="1" fragment="single">NEATIALVKGRNIQILKRLTSGVPGKFKAGGQSARRLERLIDLAAHEFLQRVGQKANEQFLPLLQEKKLKGILVGGPGHTKLEFVQKDYLHHELKKLVLDTFDLCYTEEFGIRELLEKAAPLLKDVELMKERQAVQRFLKELVKEDGGLACYGEKEVLNALMMGAVDTLIVSEDIERYKVKIACNKCDYFEERTVDKIEKMKLDEEIKNMSCPKCGSALSIVEEKDYIEYLSELCEQSGAKLITVSSETEEGAMISKAFKGIAAILRYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPF6</accession>
    <name>N6VPF6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Ribosomal-protein-alanine acetyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_06030</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000048">
        <property type="protein sequence ID" value="ENN95770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593146.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPF6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00926"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1174"/>
    <dbReference type="OrthoDB" id="43754at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031415">
        <property type="term" value="C:NatA complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004596">
        <property type="term" value="F:protein-N-terminal amino-acid acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04301">
        <property type="entry name" value="NAT_SF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006464">
        <property type="entry name" value="AcTrfase_RimI/Ard1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045047">
        <property type="entry name" value="Ard1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000182">
        <property type="entry name" value="GNAT_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01575">
        <property type="entry name" value="rimI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091">
        <property type="entry name" value="N-TERMINAL ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23091:SF4">
        <property type="entry name" value="N-TERMINAL AMINO-ACID N(ALPHA)-ACETYLTRANSFERASE NATA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00583">
        <property type="entry name" value="Acetyltransf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51186">
        <property type="entry name" value="GNAT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N-acetyltransferase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="156" mass="18329" checksum="F8709651868D08FA" modified="2013-06-26" version="1">MIIRKFTSKDLEDVEAIEKEAFKHPYPTQLLLGLWINYPDCFYVAEVNNKVVGYIVGMMDWGNGHIVSLAVKKEYRRRGIGSLLLKTLENYYFNVAKCNYIVLEVRVSNIAARKFYYKMGYRDRKLIPRYYEDGEDAILMIKKRKNAKGVLIVSLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPR2</accession>
    <name>N6VPR2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Magnesium chelatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_05434</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunits D/I family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000039">
        <property type="protein sequence ID" value="ENN95885.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592481.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPR2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00838"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1061"/>
    <dbReference type="OrthoDB" id="78267at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd01451">
        <property type="entry name" value="vWA_Magnesium_chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041702">
        <property type="entry name" value="BchD/ChlD_VWA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052989">
        <property type="entry name" value="Mg-chelatase_DI-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35023">
        <property type="entry name" value="CHELATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35023:SF1">
        <property type="entry name" value="MG-PROTOPORPHYRIN IX CHELATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="2">
        <location>
            <begin position="97"/>
            <end position="269"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005799"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95885.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="269" mass="30566" checksum="4774F45E6C544A10" modified="2013-06-26" version="1">MFGIDKNITVNPNILNLKCKSYISESRGKHIKAMAKRGRYIKYKPTSDFEDLALDASIRRAAIYQKDRKKRYKKGLAIYLEKEDLLKKVRQRKIRSYVLFVVDASGSMAAKKRMEAAKGAVLSLLLNAYQKRNKVGMIVFRNDKADLVLPFTNSVDLAEKLLKDIPTGGKTPLGKALVKAYETIIKEMKKPIIPIMVIISDFKPTVGDIKEIDKICEMIVEKGINVILIDTETSFVKVGIGDRLAKKFGFKYYNIKDITKENILEKVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPT0</accession>
    <name>N6VPT0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05354</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95905.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592386.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPT0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01316"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1045"/>
    <dbReference type="OrthoDB" id="103508at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039967">
        <property type="entry name" value="MJ1020-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40517">
        <property type="entry name" value="METAL-DEPENDENT PHOSPHOHYDROLASE, HD SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40517:SF1">
        <property type="entry name" value="METAL-DEPENDENT PHOSPHOHYDROLASE, HD SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="47"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95905.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="255" mass="28793" checksum="666EEAB9D487B5E9" modified="2013-06-26" version="1">MVYNELENLNGVAKKVYNDLLSNKKVNTFLKMSNIMAVGRLGYNDHGKTHAKIVANNAIKMLKILYKRGIKPSFMEDCKGSFEDSVVITLLGAYLHDIGNAIHRDVHHLHSSYLAKDIIEEILMKYYSEEKVYQMLTEILHIIYSHSETIMSLTLEAGVVAVADGTDMTKGRSRVPINKGCYDIHSISSASVEKVIIKEGKEKPIKIEVILSNEAGIFQIQEVLGEKIKWSGIKQYISVYAKVMKEKPVFREISL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPT4</accession>
    <name>N6VPT4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Fe3+-hydroxamate ABC transporter periplasmic protein-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05284</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000036">
        <property type="protein sequence ID" value="ENN95910.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPT4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00454"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1032"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000272">
        <property type="term" value="P:polysaccharide catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1330.10">
        <property type="entry name" value="Dockerin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050902">
        <property type="entry name" value="ABC_Transporter_SBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002491">
        <property type="entry name" value="ABC_transptr_periplasmic_BD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036439">
        <property type="entry name" value="Dockerin_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018247">
        <property type="entry name" value="EF_Hand_1_Ca_BS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535:SF34">
        <property type="entry name" value="MOLYBDATE-BINDING PROTEIN MOLA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30535">
        <property type="entry name" value="VITAMIN B12-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01497">
        <property type="entry name" value="Peripla_BP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53807">
        <property type="entry name" value="Helical backbone' metal receptor"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63446">
        <property type="entry name" value="Type I dockerin domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00018">
        <property type="entry name" value="EF_HAND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50983">
        <property type="entry name" value="FE_B12_PBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fe/B12 periplasmic-binding" evidence="1">
        <location>
            <begin position="128"/>
            <end position="400"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50983"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95910.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="425" mass="48879" checksum="9BCE4725F79663FF" modified="2013-06-26" version="1">MNKDGYIDISDVIYLFKHRDVPLTDGDLNCDNSVDIADVVYLFRNYDKFREPVKYAKNIKIKYYDPYGHEVNPYEGDKYAYKVLIDAKDQKFVLINRSTPQTEAENYIKLAKSQYGNDVKVLYVPLKRVVIMSSTHIALMEPLNNDGSVISSVKGIMWGGGYTWYFNDIKKGLENGSIIDVGSSWNPNWDKIINLTPEAIIVYPGYSGDAIIEKCKELNLTYIADSEYLENSFLARFEWIKFFAALYNKEDVAKEYFTKVEKNALNVRRITRNGDYVLVAWGKNYPSYGGTYVPKAQSYVAKGIMEDCHADYIFKDIPGTGSACINYETFADRAKNADIWVIPSNTKWLTTFKNDHPGYKDFKAVKNGRVFCISDDYWQIGLMKTDELLYDLATIIHPELFKGRTTHFFLKYNPEDNTAKPYTAN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPV3</accession>
    <name>N6VPV3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DNA-directed RNA polymerase</fullName>
            <ecNumber evidence="2">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">J422_05169</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The Rpo2 subunit (Rpo2N and Rpo2C in this organism) is implicated in DNA promoter recognition and in nucleotide binding.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000033">
        <property type="protein sequence ID" value="ENN95930.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592243.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPV3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01223"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1011"/>
    <dbReference type="OrthoDB" id="371768at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1100.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1110.10">
        <property type="entry name" value="RNA polymerase Rpb2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007644">
        <property type="entry name" value="RNA_pol_bsu_protrusion"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007642">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037034">
        <property type="entry name" value="RNA_pol_Rpb2_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007645">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007175">
        <property type="entry name" value="PRK09606.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04563">
        <property type="entry name" value="RNA_pol_Rpb2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04561">
        <property type="entry name" value="RNA_pol_Rpb2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04565">
        <property type="entry name" value="RNA_pol_Rpb2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 15" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="5 15" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 15" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RNA polymerase beta subunit protrusion" evidence="13">
        <location>
            <begin position="14"/>
            <end position="385"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="12">
        <location>
            <begin position="162"/>
            <end position="336"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="14">
        <location>
            <begin position="406"/>
            <end position="470"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00025096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF04561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF04563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF04565"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN95930.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="493" mass="56518" checksum="FB9A8973E332D9D3" modified="2013-06-26" version="1">MKALIDAYFKEHSLIDHQIESYNDFIERRLQEIVDEVGEIDTEIPGGYKVKLGKIRLGKPIIKEADGSVREITPMEARIRDLTYSVPIYLEMTPIIGGKEGETKEIYIGELPVMLGSKICLLYGKSREELIKLGEDPEDPFGYFIINGTERVLITQEDLIPNRIICEKDERSGKIVDVAKVFSTRHGFRALCTVERHPNGLLYVTFPSFPGQIPLVILMKALGAETDKEILEAINDERFFMEIVLNIQEIREEYNINTQEEALDYIGKRVAPGQARDYRLKRAETVLCNYLLPHLGVNKEDFKKKIKFLGIMTRNCLELYFGYRGEDDKDHYAFKRAKLAGDLMEDLFRYAFQQLVKDIKYQLERQALRNKSPSIQAAVRADVLTERIKHAMATGTWVTGKTGVSQLLDRTSYLATYSQLRRIVSPLSRSQPHFEARELHGTHWGKVCPSETPEGPNCGLVKNFALMCKITREEDDSKIIELLKSFNIKEIEN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ20</accession>
    <name>N6VQ20_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Ribose-5-phosphate isomerase A</fullName>
            <ecNumber evidence="5">5.3.1.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Phosphoriboisomerase A</fullName>
            <shortName evidence="5">PRI</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpiA</name>
        <name evidence="6" type="ORF">J422_05065</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the reversible conversion of ribose-5-phosphate to ribulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 5">
            <text>aldehydo-D-ribose 5-phosphate = D-ribulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:14657"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="EC" id="5.3.1.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Carbohydrate degradation; pentose phosphate pathway; D-ribose 5-phosphate from D-ribulose 5-phosphate (non-oxidative stage): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 5">Belongs to the ribose 5-phosphate isomerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.6" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95985.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ20"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00476"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.993"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00412"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004751">
        <property type="term" value="F:ribose-5-phosphate isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006014">
        <property type="term" value="P:D-ribose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009052">
        <property type="term" value="P:pentose-phosphate shunt, non-oxidative branch"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01398">
        <property type="entry name" value="RPI_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000018">
        <property type="entry name" value="Ribose-5-phosphate isomerase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1360:FF:000001">
        <property type="entry name" value="Ribose-5-phosphate isomerase A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00170">
        <property type="entry name" value="Rib_5P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037171">
        <property type="entry name" value="NagB/RpiA_transferase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020672">
        <property type="entry name" value="Ribose5P_isomerase_typA_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004788">
        <property type="entry name" value="Ribose5P_isomerase_type_A"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001924">
        <property type="entry name" value="PRK00702.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00021">
        <property type="entry name" value="rpiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11934:SF0">
        <property type="entry name" value="RIBOSE-5-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06026">
        <property type="entry name" value="Rib_5-P_isom_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75445">
        <property type="entry name" value="D-ribose-5-phosphate isomerase (RpiA), lid domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF100950">
        <property type="entry name" value="NagB/RpiA/CoA transferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="101"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="23"/>
            <end position="26"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="78"/>
            <end position="81"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="92"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001713"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008088"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95985.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="219" mass="23988" checksum="D730CB3508D086CC" modified="2013-06-26" version="1">MKLKVAKEAAKLIKDGMVVGLGSGSTAAMFIRELGSRIEEEELTVFGIPTSFESKMLALQYGIPLVSLDEYDVDIAIDGADEVEKETLFLIKGGGGCHTMEKIVDYNAKEFVVIVDESKIKNKLSYPIPVEVLPQAYRVVMKKLKDMGGEPIIRLAERKRGPVITDNGNMIIDVSMEIDDAVSLESEINNIPGVVENGIFTKVDKVLVATKKGIKTLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ72</accession>
    <name>N6VQ72_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 15">Phosphoribosylformylglycinamidine cyclo-ligase</fullName>
            <ecNumber evidence="4 15">6.3.3.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12 15">AIR synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13 15">AIRS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11 15">Phosphoribosyl-aminoimidazole synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="primary">purM</name>
        <name evidence="18" type="ORF">J422_04805</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="14 15">
            <text>2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + ATP = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:23032"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:137981"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.3.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 15">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the AIR synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.3.1" evidence="4 15"/>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96030.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592017.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ72"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00979"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.940"/>
    <dbReference type="OrthoDB" id="6605at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00129"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004637">
        <property type="term" value="F:phosphoribosylamine-glycine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004641">
        <property type="term" value="F:phosphoribosylformylglycinamidine cyclo-ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046084">
        <property type="term" value="P:adenine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02196">
        <property type="entry name" value="PurM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000020">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.650.10:FF:000011">
        <property type="entry name" value="Phosphoribosylformylglycinamidine cyclo-ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00741">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004733">
        <property type="entry name" value="PurM_cligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00878">
        <property type="entry name" value="purM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520:SF12">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10520">
        <property type="entry name" value="TRIFUNCTIONAL PURINE BIOSYNTHETIC PROTEIN ADENOSINE-3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 15" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 15 18" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 15" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 15" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="16">
        <location>
            <begin position="41"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="17">
        <location>
            <begin position="165"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00020367"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00031908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00033093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00049057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ENN96030.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="346" mass="38230" checksum="0DFFC5E7D6E5826D" modified="2013-06-26" version="1">MITYKDAGVDIEFEDKVIKALTSKIKFKRNDFKPVLKYHYAGAVEFGDYYLVLTTDGVGSKMIVAEMANKFDTVAIDMIAMNVNDAICIGAEPIALVDYIACGRITEEIAEQIGEGLNRGAEEANINIIGGETATLPEMVNGIDLAGTVLAIVKKDKIITGEKVKEGDVIIGLRSSGIHSNGLTLARKVLFDIAKLQVFDKLSYGKTVAEELLTPTRIYVKPVLEMVNSLDIHGLAHITGGTYRKLRRLNSDVVYYINNLPEPQPIFKEIQRLGDIEIGEMFKTFNMGIGFCVITSEDNAKDVIKISKRYGIDAQIIGEVLKEFKINNKTINGKSVIEYKDNIIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQQ8</accession>
    <name>N6VQQ8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03413</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000021">
        <property type="protein sequence ID" value="ENN96235.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQQ8"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96235.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="29" mass="3424" checksum="6B4DE24417A2E527" modified="2013-06-26" version="1" fragment="single">EILFYDLVNGVIKPTSVKKWYAIRELLKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQS4</accession>
    <name>N6VQS4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MCP methylation inhibitor CheC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_03268</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96250.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590948.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQS4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01041"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.640"/>
    <dbReference type="OrthoDB" id="182374at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17911">
        <property type="entry name" value="CheC_ClassIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="entry name" value="CheC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007597">
        <property type="entry name" value="CheC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050992">
        <property type="entry name" value="CheZ_family_phosphatases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43693">
        <property type="entry name" value="PROTEIN PHOSPHATASE CHEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43693:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE CHEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04509">
        <property type="entry name" value="CheC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="CheC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CheC-like protein" evidence="3">
        <location>
            <begin position="109"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04509"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96250.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="206" mass="23319" checksum="C3BABCD95BF7B9C9" modified="2013-06-26" version="1">MGFIKPINRLIKLGKEATENMAHSFMELTGENVEVNFLGIRFALIEYIPEQFGDENYKAARIDFEGVLSGKGLILIPEKDAIKLEKLLLIDILWDSLVSKSELPDYEEMERSLIGEVGNIVITSFLNVFANRLNGVINFTPPEFIKDIGFTIVESLITEVAEKTDVVMLYDTKVEIIGRFPLKCYLIIIVDPESIKKLDKVVDMES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQT8</accession>
    <name>N6VQT8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12 13">Ferrous iron transport protein B</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">J422_03363</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96265.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590977.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQT8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01058"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.659"/>
    <dbReference type="OrthoDB" id="85305at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015093">
        <property type="term" value="F:ferrous iron transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01879">
        <property type="entry name" value="FeoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1770">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003373">
        <property type="entry name" value="Fe2_transport_prot-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011640">
        <property type="entry name" value="Fe2_transport_prot_B_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041069">
        <property type="entry name" value="FeoB_Cyto"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050860">
        <property type="entry name" value="FeoB_GTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030389">
        <property type="entry name" value="G_FEOB_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011642">
        <property type="entry name" value="Gate_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00437">
        <property type="entry name" value="feoB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43185:SF1">
        <property type="entry name" value="FE(2+) TRANSPORTER FEOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43185">
        <property type="entry name" value="FERROUS IRON TRANSPORT PROTEIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07664">
        <property type="entry name" value="FeoB_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17910">
        <property type="entry name" value="FeoB_Cyto"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02421">
        <property type="entry name" value="FeoB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07670">
        <property type="entry name" value="Gate"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51711">
        <property type="entry name" value="G_FEOB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="10 14" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="9" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0410">Iron transport</keyword>
    <keyword evidence="15" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11 16" id="KW-0472">Membrane</keyword>
    <keyword evidence="15" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 16" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 16" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="262"/>
            <end position="281"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="318"/>
            <end position="347"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="367"/>
            <end position="389"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="401"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="433"/>
            <end position="453"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="491"/>
            <end position="510"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="522"/>
            <end position="542"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="549"/>
            <end position="568"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="588"/>
            <end position="607"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="16">
        <location>
            <begin position="619"/>
            <end position="641"/>
        </location>
    </feature>
    <feature type="domain" description="FeoB-type G" evidence="17">
        <location>
            <begin position="1"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="32"/>
            <end position="36"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="53"/>
            <end position="56"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <begin position="113"/>
            <end position="116"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00031200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="NCBIfam" id="TIGR00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR603373-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR603373-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="PROSITE" id="PS51711"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ENN96265.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="642" mass="72130" checksum="E384583E8935D2E3" modified="2013-06-26" version="1">MRIALIGNPNVGKSTLFNLLTGGRVYVGNWPGVTVEKKEGVFICGDEKIEIIDLPGVYNLSGSAMDEIVVKKYFLTEKPDLIVNIADINTLDRDLYLTLELLDTGYNVLLVLNKIDSTNVIVDEKKLKELLGIDVVKISAKKNIGIENLKKKILENLKNKRKPKIRYNKEIEDIINKIIEILKRDENLKNYNLRFLAVKLLENDQIVKEIVKNSKVSKDIEELLKNIKVDISKERYKIIDKIVKECIKREEKRDITDLLDRIFTHEVYGILALIPIVWILFKITFDFAQPFVDIIDYFISYLADYASKSISDKVLASLVADGIIGGVGSVLTFLPNIAFLFISLSILGETGLLARLPLLTEKILNKFGLPGLAILPIIMSFGCNVPGILATRIIENERDRLVTILINPFITCPARLPALIILSALLFPKNPGLVLLLMYFVGIAAALFSALLFRKAIFKDQPEPLIIELPKYHFPNIKMALKNAWENIYSFIRKAGTVILFGSILIWFLSSFGPSGYVSDISLSYSAIIGKYLSYIFQIFGWDWKLSSALLYGFIAKENVVSTLAILYGVGEESLKATLSTLPKASLLSYMVFFTLYIPCLATIAVIKQEAGWKWAIFSVIYSTFVAFIAALIVYILGIIIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQU4</accession>
    <name>N6VQU4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 6">UTP--glucose-1-phosphate uridylyltransferase</fullName>
            <ecNumber evidence="2 6">2.7.7.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">UDP-glucose pyrophosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_03388</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5 6">
            <text>alpha-D-glucose 1-phosphate + UTP + H(+) = UDP-alpha-D-glucose + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19889"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46398"/>
            <dbReference type="ChEBI" id="CHEBI:58601"/>
            <dbReference type="ChEBI" id="CHEBI:58885"/>
            <dbReference type="EC" id="2.7.7.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the UDPGP type 2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.9" evidence="2 6"/>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96270.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590987.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQU4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01063"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.665"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003983">
        <property type="term" value="F:UTP:glucose-1-phosphate uridylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006011">
        <property type="term" value="P:UDP-alpha-D-glucose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02541">
        <property type="entry name" value="UGPase_prokaryotic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005771">
        <property type="entry name" value="GalU_uridylyltTrfase_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01099">
        <property type="entry name" value="galU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197">
        <property type="entry name" value="UTP--GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43197:SF1">
        <property type="entry name" value="UTP--GLUCOSE-1-PHOSPHATE URIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="7">
        <location>
            <begin position="4"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012415"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00048128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU361259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96270.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="283" mass="31836" checksum="47CD384FF52FF976" modified="2013-06-26" version="1">MVKKAIIPVAGFGTRLLPITKAQPKEMLPVVNKPIVQYVVEDLVEAGVKNILFVTGKGKQAIENHFDINYELECKLKASGKNSLLKILKEIDNLGNIYYVRQKEPKGLGDAILYGENFVGNEYFIVMVGDTIYSENIVFDLLKAYKKYGCSVIALERVPKEEVYKYGVIEGEKIEDGIYKIKSMVEKPSVEEAPSNLIITGAYLFSPKIFEKIKETSPGKGGEIQITDAMNLLLKEEDIVGVEIKCKRYDIGDVLGWLKANVEIASEKIPEFREFLKEFVKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQZ1</accession>
    <name>N6VQZ1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">CCA-adding enzyme</fullName>
            <ecNumber evidence="12">2.7.7.72</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">CCA tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">tRNA CCA-pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">tRNA adenylyl-/cytidylyl- transferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">tRNA nucleotidyltransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">tRNA-NT</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">cca</name>
        <name evidence="16" type="ORF">J422_03134</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the addition and repair of the essential 3'-terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing inorganic pyrophosphate. tRNA 3'-terminal CCA addition is required both for tRNA processing and repair. Also involved in tRNA surveillance by mediating tandem CCA addition to generate a CCACCA at the 3' terminus of unstable tRNAs. While stable tRNAs receive only 3'-terminal CCA, unstable tRNAs are marked with CCACCA and rapidly degraded.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-tyrosyl-[protein] + ATP = O-(5'-adenylyl)-L-tyrosyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:54288"/>
            <dbReference type="Rhea" id="RHEA-COMP:10136"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:46858"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="EC" id="2.7.7.108"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>O-(5'-adenylyl)-L-tyrosyl-[protein] + ATP = O-[5'-(adenylyl-(5'-&gt;3')-adenylyl)]-L-tyrosyl-[protein] + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:66528"/>
            <dbReference type="Rhea" id="RHEA-COMP:13846"/>
            <dbReference type="Rhea" id="RHEA-COMP:17046"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83624"/>
            <dbReference type="ChEBI" id="CHEBI:167160"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a tRNA precursor + 2 CTP + ATP = a tRNA with a 3' CCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14433"/>
            <dbReference type="Rhea" id="RHEA-COMP:10465"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:74896"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="EC" id="2.7.7.72"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>a tRNA with a 3' CCA end + 2 CTP + ATP = a tRNA with a 3' CCACCA end + 3 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:76235"/>
            <dbReference type="Rhea" id="RHEA-COMP:10468"/>
            <dbReference type="Rhea" id="RHEA-COMP:18655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:83071"/>
            <dbReference type="ChEBI" id="CHEBI:195187"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="12">A single active site specifically recognizes both ATP and CTP and is responsible for their addition.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Archaeal CCA-adding enzyme subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.72" evidence="12"/>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96320.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590782.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQZ1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00173"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.615"/>
    <dbReference type="OrthoDB" id="7378at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070733">
        <property type="term" value="F:AMPylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004810">
        <property type="term" value="F:CCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160016">
        <property type="term" value="F:CCACCA tRNA nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042245">
        <property type="term" value="P:RNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001680">
        <property type="term" value="P:tRNA 3'-terminal CCA addition"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd05400">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1550">
        <property type="entry name" value="Archaeal tRNA CCA-adding enzyme catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.460.10">
        <property type="entry name" value="Beta Polymerase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1410.30">
        <property type="entry name" value="CCA tRNA nucleotidyltransferase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.590">
        <property type="entry name" value="Poly(A) polymerase predicted RNA binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01264">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048833">
        <property type="entry name" value="CAA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008229">
        <property type="entry name" value="CCA-adding_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042090">
        <property type="entry name" value="CCA_tRNA_nucleotrans_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006116">
        <property type="entry name" value="NT_2-5OAS_ClassI-CCAase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043519">
        <property type="entry name" value="NT_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011068">
        <property type="entry name" value="NuclTrfase_I-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002934">
        <property type="entry name" value="Polymerase_NTP_transf_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015329">
        <property type="entry name" value="tRNA_NucTransf2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03671">
        <property type="entry name" value="cca_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643">
        <property type="entry name" value="CCA-ADDING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39643:SF1">
        <property type="entry name" value="CCA-ADDING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21133">
        <property type="entry name" value="CAA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01909">
        <property type="entry name" value="NTP_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09249">
        <property type="entry name" value="tRNA_NucTransf2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005335">
        <property type="entry name" value="CCA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81301">
        <property type="entry name" value="Nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55003">
        <property type="entry name" value="PAP/Archaeal CCA-adding enzyme, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81631">
        <property type="entry name" value="PAP/OAS1 substrate-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50152">
        <property type="entry name" value="25A_SYNTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 12 16" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12" id="KW-0692">RNA repair</keyword>
    <keyword evidence="9 12" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 12 16" id="KW-0808">Transferase</keyword>
    <keyword evidence="2 12" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Polymerase nucleotidyl transferase" evidence="13">
        <location>
            <begin position="36"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA nucleotidyltransferase substrate binding" evidence="14">
        <location>
            <begin position="153"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="domain" description="CCA-adding enzyme C-terminal" evidence="15">
        <location>
            <begin position="288"/>
            <end position="401"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="52"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="63"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="139"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF01909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF09249"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF21133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN96320.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="426" mass="50390" checksum="1089E0BA95A21D4C" modified="2013-06-26" version="1">MLEEILKRIRPTEEEIKEISNLAENVIKNIYDVANEKGYNILEALWLGSSARNTHLKGDHDIDIFILFDKSVSIDELEVIALDIGKEVIKRLNGKCQINYASHPYIQGIVGKYKVDIVPCYKINFGEKIISAVDRTPLHHKFLIERLDEKLCDEVRLLKAFLKSLNLYGSDVKTKGFSGYLCELLILHYKSFLNLLNDAKDWGERKKIILNDIFELYKNPKFREFDEPLIVYDPVDLNRNVAAPLSRENYCKFIFFAREFLKNMNEEFFNDYYKKLEEKLENRDKGFILTLKFPKTEVDDIIYPQLEKFQKSLNKLIEQNEFKILNCKCYTDSDYAYIEWEFLVYQLPKIKLRDGPEVFEKERVEKFIKKYDKVFIKGCKLYAFTERKYCHILELFKDIIEGKIKNISKPKHIDIKSGVILWKQAT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR16</accession>
    <name>N6VR16_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Heat shock protein Hsp20</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_02679</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the small heat shock protein (HSP20) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96350.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590579.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR16"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00088"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.525"/>
    <dbReference type="OrthoDB" id="198277at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06464">
        <property type="entry name" value="ACD_sHsps-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.790">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002068">
        <property type="entry name" value="A-crystallin/Hsp20_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008978">
        <property type="entry name" value="HSP20-like_chaperone"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031107">
        <property type="entry name" value="Small_HSP"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11527">
        <property type="entry name" value="HEAT-SHOCK PROTEIN 20 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00011">
        <property type="entry name" value="HSP20"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49764">
        <property type="entry name" value="HSP20-like chaperones"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01031">
        <property type="entry name" value="SHSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0346">Stress response</keyword>
    <feature type="domain" description="SHSP" evidence="3">
        <location>
            <begin position="37"/>
            <end position="148"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="RuleBase" id="RU003616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS01031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96350.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="148" mass="16710" checksum="C6387F6F5CE84143" modified="2013-06-26" version="1">MFGRDPFDSLFERMFREFFSMPMGSSTTIMQTSRGIQISGSGFMPISIIEGDDHLKVIAWLPGVNKEDIVLNAVGDSLEIRAKRSPLMITESERIIYSEIPEEEEIYRNIKLPAVVKEDKASAKFENGVLTVILPKAESSRRKGIRID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR23</accession>
    <name>N6VR23_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_07142</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000076">
        <property type="protein sequence ID" value="ENN95566.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR23"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1380"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008713">
        <property type="term" value="F:ADP-heptose-lipopolysaccharide heptosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002201">
        <property type="entry name" value="Glyco_trans_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051199">
        <property type="entry name" value="LPS_LOS_Heptosyltrfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30160">
        <property type="entry name" value="TETRAACYLDISACCHARIDE 4'-KINASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01075">
        <property type="entry name" value="Glyco_transf_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95566.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="77" mass="8949" checksum="FF28E44846963F4D" modified="2013-06-26" version="1">MHLAAAVNEKIISIFGPTHPSEKAPLHKESIYLWKQVGCNPCYDLYGRHPNPCPYKKKCMREIKVEEVLDFIFKMTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR79</accession>
    <name>N6VR79_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein eL14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl14e</name>
        <name evidence="5" type="ORF">J422_06648</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL14 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000057">
        <property type="protein sequence ID" value="ENN95646.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593894.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR79"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00721"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1294"/>
    <dbReference type="OrthoDB" id="63594at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042273">
        <property type="term" value="P:ribosomal large subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06088">
        <property type="entry name" value="KOW_RPL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000045">
        <property type="entry name" value="50S ribosomal protein L14e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00721">
        <property type="entry name" value="Ribosomal_eL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039660">
        <property type="entry name" value="Ribosomal_eL14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023651">
        <property type="entry name" value="Ribosomal_eL14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041985">
        <property type="entry name" value="Ribosomal_eL14_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003320">
        <property type="entry name" value="PRK04333.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11127:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="KOW" evidence="4">
        <location>
            <begin position="6"/>
            <end position="36"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00721"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95646.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="80" mass="8761" checksum="0B5F7C1D3C60E9C4" modified="2013-06-26" version="1">MAAIEVGRVCLKTAGREAGKICVIVDILDKNFVVVDGMVKRRRCNIKHLEPTEKKVDIKPGATTEEVKMALEAAGLLKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR96</accession>
    <name>N6VR96_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YjbQ family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02330</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0047 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96425.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR96"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00345"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.458"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.460">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001602">
        <property type="entry name" value="UPF0047_YjbQ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035917">
        <property type="entry name" value="YjbQ-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00149">
        <property type="entry name" value="TIGR00149_YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN YJBQ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30615:SF8">
        <property type="entry name" value="UPF0047 PROTEIN C4A8.02C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01894">
        <property type="entry name" value="YjbQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004681">
        <property type="entry name" value="UCP004681"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111038">
        <property type="entry name" value="YjbQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96425.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="126" mass="14319" checksum="FCEFB05396392E64" modified="2013-06-26" version="1">MLFTYTVRSKKREELIDITNLLLDAIKRSGVNEGFAIIYAPHTTAAITINENADPTVKGDIINYLSKLIPKNYNYKHLEGNSDAHIKSSIIGCSEMIIIKDGKPILGTWQVYFLQNLMDQEKEKFI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR97</accession>
    <name>N6VR97_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker protein 14</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06598</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000055">
        <property type="protein sequence ID" value="ENN95676.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593727.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR97"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00600"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1284"/>
    <dbReference type="OrthoDB" id="114017at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008303">
        <property type="entry name" value="Methan_mark_14"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03285">
        <property type="entry name" value="methan_mark_14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09887">
        <property type="entry name" value="DUF2114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016937">
        <property type="entry name" value="UCP016937"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95676.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="482" mass="52048" checksum="6B25BA13A065C7E1" modified="2013-06-26" version="1">MLDKILSIFKRKPKIAYAKSQNVDLFELKRGPYYIVASVELGNTTTKSIITATNLETGKSYIISKAVRMTRDVRKPKKGEKVFGKTLWGVELTREAIAEMVSEVLLESLDKANLTINDLDFVVRSTGVTAGFASPEEVSEVIIALAQGCMKAGVPPAKMTPAMSKDQLPEKVRKYSFLDKIIFDGAVTGVLPPTGKEVVANEMEGELVTAGVKVGSKWTFVDFRNPCMSIDFGTTLAGRITNDTLPYAKVVGNLCGLAGAIADAIARGTGKIDEKTGAVLDLKADKGKADEKLAKEYADKMHKYIIIDLVPDGVSRFGTVPVNTEAAKKAGVKLLGCDVGENGSDLTKLEELGKELYEKSNLSTLMLCLDYVMAEIVRRLVDLAHREGLISEKTAIGITGRAGITGKKPEIIIEKLKSLGIWENVEENVVFVEDGLALGAAVMARCMNCLGTPKVPIGGNRGGGCILGLRIKWQKEKGMIRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRA9</accession>
    <name>N6VRA9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Tryptophan--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tryptophanyl-tRNA synthetase</fullName>
            <shortName evidence="8">TrpRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trpS</name>
        <name evidence="10" type="ORF">J422_06361</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the attachment of tryptophan to tRNA(Trp).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>tRNA(Trp) + L-tryptophan + ATP = L-tryptophyl-tRNA(Trp) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24080"/>
            <dbReference type="Rhea" id="RHEA-COMP:9671"/>
            <dbReference type="Rhea" id="RHEA-COMP:9705"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78535"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.2"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.2" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95691.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593516.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRA9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01301"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1238"/>
    <dbReference type="OrthoDB" id="371821at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004830">
        <property type="term" value="F:tryptophan-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006436">
        <property type="term" value="P:tryptophanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00806">
        <property type="entry name" value="TrpRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000207">
        <property type="entry name" value="Tryptophan--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.240.10">
        <property type="entry name" value="Tyrosyl-Transfer RNA Synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00140_A">
        <property type="entry name" value="Trp_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002305">
        <property type="entry name" value="aa-tRNA-synth_Ic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002306">
        <property type="entry name" value="Trp-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020653">
        <property type="entry name" value="Tryptophan-tRNA-ligase_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008925">
        <property type="entry name" value="PRK12285.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00233">
        <property type="entry name" value="trpS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055:SF5">
        <property type="entry name" value="TRYPTOPHAN--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10055">
        <property type="entry name" value="TRYPTOPHANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00579">
        <property type="entry name" value="tRNA-synt_1b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01039">
        <property type="entry name" value="TRNASYNTHTRP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="68"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="8">
        <location>
            <begin position="244"/>
            <end position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00140"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95691.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="358" mass="41570" checksum="A672275844F435C7" modified="2013-06-26" version="1">MLTPWDVPEIIDYKKTMEQFGIKEITDEMKDLHHFFRRNIILGHRDFDKVLEAIKNKNDFAVVSGMMPSGKMHFGHKMVVDMLKFYQKYTDEIYIPIADLEAYWARNISFEKAKDIAIDYIANYIALGLDVERVNIYLQSKNWKVKDLALILAKKTNWNELKSIYGFGGDTNIGHLFAPIVQVADILQAQFDNNFEKIPKITLVPVGLDQDPHIRLTRDIANRFKITPPSATYHRFMRGLLGGKMSSSKPETAIFLSDDNETVKKKIFSAKTGGRESLEEQRKFGGRPEECIVYELFLYHLILDDEELKNIYEKCKSGELMCKECKKMAYNLITEFLNDLKEKRDIELAKKIVEKCWG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRM2</accession>
    <name>N6VRM2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">TIGR00267 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_05688</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRM2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01265"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1109"/>
    <dbReference type="OrthoDB" id="60696at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005384">
        <property type="term" value="F:manganese ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030026">
        <property type="term" value="P:intracellular manganese ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02437">
        <property type="entry name" value="CCC1_like_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008217">
        <property type="entry name" value="Ccc1_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006682">
        <property type="entry name" value="CHP00267"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00267">
        <property type="entry name" value="TIGR00267 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31851">
        <property type="entry name" value="FE(2+)/MN(2+) TRANSPORTER PCL1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01988">
        <property type="entry name" value="VIT1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="38"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="98"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="127"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="161"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="180" mass="19157" checksum="BC9CD75984AFCFD9" modified="2013-06-26" version="1">MEKIADKPMVRYIIRGLIDGTLSTLGVVIGASGADSHIIISAGLGGGLANGLSNILGAFTAEKASLERERINMERKLVKNHGYLKKSLIYKNALKDTMICGVIDGISTTLGSALPVLPFFFLKPNVALYVAIGITVGLLFLLGIFIGKISKENLLISGTKMVVGALLVSFLCFIIENIFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRN3</accession>
    <name>N6VRN3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">TIGR02757 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05753</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95816.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592808.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRN3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01254"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1122"/>
    <dbReference type="OrthoDB" id="382774at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014127">
        <property type="entry name" value="CHP02757"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09674">
        <property type="entry name" value="DUF2400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95816.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="265" mass="31453" checksum="3403A11C75B590AF" modified="2013-06-26" version="1">MLIKDYIERYSKEIDLLLNPNGNPPLPFNPYKDEKNFVAMYYLQLASIDQAQIVGTAENVRKLMVNLYNIFGEKLFNIKEESIFLKTLKSIKEKEKIRLGKLWGRIPKILVDVNNYLSDIDYDLLTHTKNFEKPINFVNDLANKIYMMGKSKESAKKKAWMYMRWMVREYPDLKIFHFDPADLYIPVDRHVAKVGYCLSVLDNYNSNWKNVVKMTRFAKSLYKKDPAKIDYPFFLLGREIGKDILLKEKKERRKMLENIILSKLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRS6</accession>
    <name>N6VRS6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">fructose-bisphosphate aldolase</fullName>
            <ecNumber evidence="2">4.1.2.13</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_05449</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DeoC/FbaB aldolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.13" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000041">
        <property type="protein sequence ID" value="ENN95866.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592533.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRS6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01399"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1064"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd00958">
        <property type="entry name" value="DhnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050456">
        <property type="entry name" value="DeoC/FbaB_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005321">
        <property type="entry name" value="PRK06852.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE CLASS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916:SF4">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE CLASS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor" evidence="5">
        <location>
            <position position="170"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with dihydroxyacetone-P" evidence="5">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="6">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013068"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PIRSR" id="PIRSR038992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95866.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="292" mass="32369" checksum="73C8575C7F16171D" modified="2013-06-26" version="1" fragment="single">KKDIKVPLTVPEYAKKEYIENYLELTKGTGNLMIFAGDQKIEHMNDDFFGEGIAKDDADPEHLFRIAEGGKICAFATQLGLIARYGMDYDVPYIVKINSKTHLVKTRDPISRALVDMEDVISLKERGVNILGVGYTIYPGSEYEHIMFREASKIILEAHKHGLIAILWSYPRGKNVKNEFDPHIIAGAAGIACCLGADFVKVNYPGDPESFKEAVLAAGRTGVLCAGGKSKDPKEFLKDIWEQINISGARGNATGRNIHQKPLEKAIRMCNAIYAITIEGKSLEEALKIYYG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRV7</accession>
    <name>N6VRV7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_05309</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95896.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592375.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRV7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01325"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1036"/>
    <dbReference type="OrthoDB" id="359310at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012022">
        <property type="entry name" value="UCP005295"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42658">
        <property type="entry name" value="HYDROLASE TATD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42658:SF1">
        <property type="entry name" value="HYDROLASE TATD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005295">
        <property type="entry name" value="UCP005295_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01137">
        <property type="entry name" value="TATD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR005295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95896.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="250" mass="28376" checksum="8F0AAFB98120E794" modified="2013-06-26" version="1">MIIDTHTHLDTRGYEDLELLAMTLDKAITLAHDPFPMRTVEVFITHIEKVINEIERAKKAGLMVYCALGMHPRALTKNIDEALERLKSYLDKAIAIGEIGLEKATKEEKEAFEKQLLLANDIEKPAIIHTPRRDKREVTKEIIDILSSIDLKVPVIVDHCNKDIINIVLDNNYYAGLTVQPGKLTPMEAVEIIKEYKDFSDRIVLNSDLSSNPSDVLAVPRTVLKLKINNIEKEIIEKVSYKNAQSIFSF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRW3</accession>
    <name>N6VRW3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Translation-associated GTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_05334</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the RelA/SpoT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95901.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592380.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRW3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01320"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1041"/>
    <dbReference type="OrthoDB" id="5875at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01669">
        <property type="entry name" value="TGS_MJ1332_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01899">
        <property type="entry name" value="Ygr210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.30:FF:000002">
        <property type="entry name" value="GTP pyrophosphokinase (RelA/SpoT)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.470">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031167">
        <property type="entry name" value="G_OBG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004095">
        <property type="entry name" value="TGS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012676">
        <property type="entry name" value="TGS-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013646">
        <property type="entry name" value="YGR210-like_G4"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007171">
        <property type="entry name" value="PRK09602.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305">
        <property type="entry name" value="OBG GTPASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23305:SF1">
        <property type="entry name" value="OBG-TYPE G DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02824">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08438">
        <property type="entry name" value="YGR210-like_G4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81271">
        <property type="entry name" value="TGS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51710">
        <property type="entry name" value="G_OBG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51880">
        <property type="entry name" value="TGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="OBG-type G" evidence="4">
        <location>
            <begin position="2"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="domain" description="TGS" evidence="5">
        <location>
            <begin position="317"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="157"/>
            <end position="184"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007476"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95901.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="395" mass="45035" checksum="2004223A8EC4FB93" modified="2013-06-26" version="1">MSMIGLVGKPNVGKSTMFTALTEKDAEIGNYPFTTIEPNKGVAYLTKECPCKELGVKCNPRNSKCIDGIRYIPVEVIDVAGLVPKAYEGRGMGNKFLDDLRKADALILVVDASGRTDKEGNIGEGDPIEDVKFLLNEIDMWIYKILTRNWDRLSRRAQQEKNIVKVLKEQLSGLNIKEEDIKEAIRDFDESPIKWKDEDLLLLAKRLRLISKPIIIAANKADLPEAKKNIERMKKEFKEYIIVPTSAEIELALKRAEKAGLIKRIFREGKEDFIILDESKLTNEMRKAFEYIKNFLEKYGSTGVREVLNRAYFDLLNMIVVYPVEDENRFSDKQGNVLPDAYLMKKGSTARDLAYKIHTDLGEGFIYAIDARRKIRVGADYELKDNDIIKIVSAR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRX3</accession>
    <name>N6VRX3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05254</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000035">
        <property type="protein sequence ID" value="ENN95911.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592274.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRX3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00738"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1026"/>
    <dbReference type="OrthoDB" id="258845at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10770">
        <property type="entry name" value="Hypothetical protein VC1899 like domain (Restriction endonuclease-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95911.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="496" mass="58213" checksum="5A65BA5825A0F536" modified="2013-06-26" version="1">MRVWISTVGSSPLAVFNTLWMAIVKEGYIPDKVYLLWNYKVEKNKEIIKNYIKSLNESYNFNIDVDESYKVSEDDFKDFSNALKEIIVKEKKRGNEVAIDMTPGRKFMSALSMFAGIEGVSDGKNKYKCDKVYYLHLKDMGYINYPLFLIPFSIQELHEFISELSDGKEDNKVLKKEELEDLAISRKEILAIINQYFLFGKSNFEIKVENQKFAAISLYENDNKAKVEINKDLKLSYYVGDCESIETILNASGLKEIKFIVYDDEKEEFNEEEFYKWLFKKLKEKETYYITFDTNALINQIPRKIIDYLDKQREKISPNFVISQCVINELTNERGKIKDCEDRDFINQPSSIDRIFKLGLSQIRLLNNRNAIIIPGKGVWDNNIKESFIKFSDEHKGKLLILTSDKNFTHNLQYLPDIIPIYIEFGELKKLVKWENVRDLIYISSIVFGKIHVKPISQVLGIWRGKNPIDWDEEKILFRKINVMCKHAIDVLRNIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS00</accession>
    <name>N6VS00_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">ADP-specific phosphofructokinase</fullName>
            <ecNumber evidence="7">2.7.1.146</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ADP-dependent phosphofructokinase</fullName>
            <shortName evidence="7">ADP-Pfk</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pfkC</name>
        <name evidence="8" type="ORF">J422_04820</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the phosphorylation of fructose 6-phosphate to fructose 1,6-bisphosphate using ADP as the phosphate donor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>beta-D-fructose 6-phosphate + ADP = beta-D-fructose 1,6-bisphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20105"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.1.146"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="7">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="7">Carbohydrate degradation; glycolysis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the carbohydrate kinase PfkC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.146" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95936.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592044.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS00"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00526"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.944"/>
    <dbReference type="OrthoDB" id="85200at2157"/>
    <dbReference type="UniPathway" id="UPA00109"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043844">
        <property type="term" value="F:ADP-specific phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008443">
        <property type="term" value="F:phosphofructokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006000">
        <property type="term" value="P:fructose metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1190.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00561">
        <property type="entry name" value="ADP_PFKinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007666">
        <property type="entry name" value="ADP_PFK/GK"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015990">
        <property type="entry name" value="ADP_PFK/GK_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011790">
        <property type="entry name" value="ADP_PFK_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029056">
        <property type="entry name" value="Ribokinase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02045">
        <property type="entry name" value="P_fruct_ADP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21208">
        <property type="entry name" value="ADP-DEPENDENT GLUCOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21208:SF1">
        <property type="entry name" value="ADP-DEPENDENT GLUCOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04587">
        <property type="entry name" value="ADP_PFK_GK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015883">
        <property type="entry name" value="ADP-Pfk_glckin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53613">
        <property type="entry name" value="Ribokinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51255">
        <property type="entry name" value="ADPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="4 7 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 8" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="7">
        <location>
            <position position="425"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="425"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00561"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95936.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="443" mass="51391" checksum="41AE27B93C6DCBF3" modified="2013-06-26" version="1">MKMLEKIRKVKLFTAYNANVDAIKYLNSNDVQRLVDMFDHKEIIRRMEEYPRVIEEPLDFVARLVYSIKTGKPAEVPIVEEMNEWFNKLKYDEERMGGQAGIVANLMATLNIDRVITYVPLLSKKQAEMFHENVLYPKLENNKLVFKKIRESYEDVPTKINRIFEFKKGLKFKLGNEEIVAKESTRFIAASRPESYRIGIRDELRPYLPQFGVDCAFLSGYQGIKEKYKDGKTAEYYFEKAKEDIKLLKEGNIMTHLEFASISNIKIREMVVNYILSEVNSVGMDETEIANVLHILGYEDLSDKIIKNSFVEDIVEGSKILLDKFNNLEIIQVHTIYFILFVCRKDNPLDLKELKECLDFSTILAATRAKLGKIESVDDLKEGLKISYNKYRDMYDKCMIEDDYKIAITPSRYVENPKSTVGLGDTISSGAFVYYNALIKNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS46</accession>
    <name>N6VS46_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0215 protein J422_05020</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05020</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0215 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95976.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592125.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS46"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00485"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.984"/>
    <dbReference type="OrthoDB" id="15207at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2170.10">
        <property type="entry name" value="archaeoglobus fulgidus dsm 4304 superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00582">
        <property type="entry name" value="UPF0215"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002802">
        <property type="entry name" value="Endo_dU"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518">
        <property type="entry name" value="UPF0215 PROTEIN MJ1150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39518:SF2">
        <property type="entry name" value="UPF0215 PROTEIN MJ1150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01949">
        <property type="entry name" value="Endo_dU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006380">
        <property type="entry name" value="UCP006380"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95976.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="182" mass="21015" checksum="0778A2BC40400BAF" modified="2013-06-26" version="1">MKDQIEVIGFDDAPFKREDREGILISVYMRGNKIIDGIYFKRIKKDGLDVTEKIIEIVKGKHYKKIKAIFLSGVTFAGFNIANINEIYSKTGKPVIVVIDKNPNKDKIFLALKKHFCDAEIRIRIINSLPNPEKLENIYVQYIGCDRNFVKNMIKKTKLKSKIPECLRIAHLIGRGFLELME</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS53</accession>
    <name>N6VS53_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0235 protein J422_00671</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00671</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0235 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589810.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS53"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00901"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.129"/>
    <dbReference type="OrthoDB" id="53248at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1200.10">
        <property type="entry name" value="YggU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00634">
        <property type="entry name" value="UPF0235"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003746">
        <property type="entry name" value="DUF167"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036591">
        <property type="entry name" value="YggU-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00251">
        <property type="entry name" value="DUF167 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13420">
        <property type="entry name" value="UPF0235 PROTEIN C15ORF40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13420:SF7">
        <property type="entry name" value="UPF0235 PROTEIN C15ORF40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02594">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01152">
        <property type="entry name" value="DUF167"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69786">
        <property type="entry name" value="YggU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="97" mass="11151" checksum="E2662E8CB879DAF4" modified="2013-06-26" version="1">MIRECKDGVIVDIEVQPNANKNEIFGINEWRKRVMVKIKAPPVEGKANKEIIKFFRKLFNSDIEILSGTTSSKKSVLIKGVKKEDVERVIKCSYQKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSC1</accession>
    <name>N6VSC1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Probable thymidylate kinase</fullName>
            <ecNumber evidence="2 11">2.7.4.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9 11">dTMP kinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tmk</name>
        <name evidence="13" type="ORF">J422_00396</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>dTMP + ATP = dTDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:13517"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58369"/>
            <dbReference type="ChEBI" id="CHEBI:63528"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.9"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the thymidylate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.9" evidence="2 11"/>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589761.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSC1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00701"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.76"/>
    <dbReference type="OrthoDB" id="43083at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004798">
        <property type="term" value="F:dTMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006233">
        <property type="term" value="P:dTDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006235">
        <property type="term" value="P:dTTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006227">
        <property type="term" value="P:dUDP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01672">
        <property type="entry name" value="TMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00165">
        <property type="entry name" value="Thymidylate_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039430">
        <property type="entry name" value="Thymidylate_kin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018095">
        <property type="entry name" value="Thymidylate_kin_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018094">
        <property type="entry name" value="Thymidylate_kinase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00041">
        <property type="entry name" value="DTMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344">
        <property type="entry name" value="THYMIDYLATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10344:SF4">
        <property type="entry name" value="UMP-CMP KINASE 2, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02223">
        <property type="entry name" value="Thymidylate_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01331">
        <property type="entry name" value="THYMIDYLATE_KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 11 13" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 11" id="KW-0545">Nucleotide biosynthesis</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 11 13" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thymidylate kinase-like" evidence="12">
        <location>
            <begin position="5"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="7"/>
            <end position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013355"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022727"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00029962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048743"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="182" mass="21373" checksum="EA7B264F2D82F1BB" modified="2013-06-26" version="1">MFIVFEGIDGSGKTTQAKLLAEKLKAFFTYEPTDGDVGKLIRAILREKRDVDNRTLALLFAADRIEHNKIIKKELKKRDVVCDRYFYSSLAYQSVSGVDEDFIKNINRYIIEPDITFLLIIDIEKALERKNKDMFENKDFLEKVQNKYLELAKEYNFIVIDANRDVEEIHRDILKHLSKHLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSH5</accession>
    <name>N6VSH5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 14">DNA ligase</fullName>
            <ecNumber evidence="14">6.5.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Polydeoxyribonucleotide synthase [ATP]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">lig</name>
        <name evidence="17" type="ORF">J422_00220</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="14">DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="14">
            <text>ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.</text>
            <dbReference type="EC" id="6.5.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1 14 15">Belongs to the ATP-dependent DNA ligase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.1" evidence="14"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96825.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589731.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSH5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01125"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.44"/>
    <dbReference type="OrthoDB" id="31274at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003910">
        <property type="term" value="F:DNA ligase (ATP) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071897">
        <property type="term" value="P:DNA biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006273">
        <property type="term" value="P:lagging strand elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07901">
        <property type="entry name" value="Adenylation_DNA_ligase_Arch_LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd07972">
        <property type="entry name" value="OBF_DNA_ligase_Arch_LigB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3260.10:FF:000007">
        <property type="entry name" value="DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.30:FF:000012">
        <property type="entry name" value="Probable DNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3260.10">
        <property type="entry name" value="DNA ligase, ATP-dependent, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00407">
        <property type="entry name" value="DNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050191">
        <property type="entry name" value="ATP-dep_DNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022865">
        <property type="entry name" value="DNA_ligae_ATP-dep_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000977">
        <property type="entry name" value="DNA_ligase_ATP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012309">
        <property type="entry name" value="DNA_ligase_ATP-dep_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012310">
        <property type="entry name" value="DNA_ligase_ATP-dep_cent"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016059">
        <property type="entry name" value="DNA_ligase_ATP-dep_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012308">
        <property type="entry name" value="DNA_ligase_ATP-dep_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036599">
        <property type="entry name" value="DNA_ligase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00574">
        <property type="entry name" value="dnl1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674:SF7">
        <property type="entry name" value="DNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45674">
        <property type="entry name" value="DNA LIGASE 1/3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04679">
        <property type="entry name" value="DNA_ligase_A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01068">
        <property type="entry name" value="DNA_ligase_A_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04675">
        <property type="entry name" value="DNA_ligase_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117018">
        <property type="entry name" value="ATP-dependent DNA ligase DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00697">
        <property type="entry name" value="DNA_LIGASE_A1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00333">
        <property type="entry name" value="DNA_LIGASE_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50160">
        <property type="entry name" value="DNA_LIGASE_A3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13 14" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="4 14" id="KW-0132">Cell division</keyword>
    <keyword evidence="8 14" id="KW-0227">DNA damage</keyword>
    <keyword evidence="11 14" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="12 14" id="KW-0234">DNA repair</keyword>
    <keyword evidence="5 14" id="KW-0235">DNA replication</keyword>
    <keyword evidence="3 14 17" id="KW-0436">Ligase</keyword>
    <keyword evidence="10 14" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-dependent DNA ligase family profile" evidence="16">
        <location>
            <begin position="325"/>
            <end position="470"/>
        </location>
    </feature>
    <feature type="active site" description="N6-AMP-lysine intermediate" evidence="14">
        <location>
            <position position="247"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="245"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="252"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="296"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="429"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="435"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007572"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="PROSITE" id="PS50160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN96825.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="570" mass="66304" checksum="75A7A1D6FD3A61C9" modified="2013-06-26" version="1">MLWKDVCKIFDNIEKTTKRLEKRDYFIKLIEMVKDPEDLKKICYIAIGRVYPEYDERELGIGEKLLINSVVSIGINKNELLEEIKKTGDIGFAIERLIGKVKHGYLLYQPLTVDEVYKTLRRVGEIEGEGSQKKKIRLISGLFLRATPIEARYLARLILEDMRIGMNVPTILEALSIYFNIPKERLEKIYAITNDIGLLAEKLLKNELDSDELKLKLFRPIKPMLAQIAPSIEQAILEMGKAQFETKFDGARVQIHKSGNNVKIYSRRLEDVTNALPEIVNAIKGINVDKLIVEGECVAIDKNTGKPRPFQDILRRFRRKYDISKFMKEINLRVYLFDILYKDGVSYIDEPFKKRREVLESIFGYENDFKKDRKRIEEELKADKIIDLSYKLVTDNPEEAREFYNWSLSIGHEGVMIKNLNAPYTPGSRVRTMYKFKPTLESLDVVITKAKRGMGKRKDWYGSFEIAVRDEEGNLYPIGHVGTGLTEEDLEYLKQRIDKIIIRDLGEEVEVKPEIVVEVAYEEIQKSDKYPCGFALRFPRVVRFREDKGVDDINTLDDVKEIYMIQRGRN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSK0</accession>
    <name>N6VSK0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">OsmC family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03768</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96146.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591342.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSK0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00386"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.737"/>
    <dbReference type="OrthoDB" id="62722at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003718">
        <property type="entry name" value="OsmC/Ohr_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036102">
        <property type="entry name" value="OsmC/Ohrsf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02566">
        <property type="entry name" value="OsmC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82784">
        <property type="entry name" value="OsmC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96146.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="179" mass="20032" checksum="EDB795EF02F92394" modified="2013-06-26" version="1">MEEICKEIAPKIMPIMLPKALENFLMKIPEKERDKLIMDIVDIIVSKTDNQEISSKFVKEFESILNIKGLKIHSRGGKGAIKEKISPMDTFLSGLCGCIAIAVGNTLLEKNIEADINVKGSVEKNFEKGCIEKIKLDIYVKPKKDVNKEELKTLVLKGSNKCLISNSLKCEVIKNVIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSL9</accession>
    <name>N6VSL9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">ABC transporter permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_03923</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0014 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96171.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591390.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSL9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00414"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.769"/>
    <dbReference type="OrthoDB" id="148038at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005226">
        <property type="entry name" value="UPF0014_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30028:SF0">
        <property type="entry name" value="PROTEIN ALUMINUM SENSITIVE 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30028">
        <property type="entry name" value="UPF0014 INNER MEMBRANE PROTEIN YBBM-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03649">
        <property type="entry name" value="UPF0014"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="26"/>
            <end position="44"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="50"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="147"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="185"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005268"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96171.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="238" mass="26310" checksum="F43979704842CDFC" modified="2013-06-26" version="1">MLYNFLFIVISAIISYIEKIGLEKEIILVSILALIQLIILGLTLKIIFSLGILFSFLLLTLMISAASYLVSKEVKIKNKSKLFLSLFLSYILTTTTVLFFLIISKTVSLTPSQIIPLAGMVIGNSMNTAHQTLETLIDKVKTEKEVFLGYIALGAREIIALKPFIRTAIRIALIPQINRTKSVGIIFIPGAMVGLILAGADPLYAAKIQVVIMWMIIYSSLVVSISICYLMHKDLAKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSM3</accession>
    <name>N6VSM3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Ribonuclease P protein component 1</fullName>
            <shortName evidence="7">RNase P component 1</shortName>
            <ecNumber evidence="7">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Rpp29</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rnp1</name>
        <name evidence="8" type="ORF">J422_03948</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic/archaeal RNase P protein component 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96176.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591398.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSM3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00419"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.774"/>
    <dbReference type="OrthoDB" id="39019at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000172">
        <property type="term" value="C:ribonuclease MRP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033204">
        <property type="term" value="F:ribonuclease P RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.210">
        <property type="entry name" value="Ribonuclease P/MRP, subunit p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00754">
        <property type="entry name" value="RNase_P_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016848">
        <property type="entry name" value="RNase_P/MRP_Rpp29-subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036980">
        <property type="entry name" value="RNase_P/MRP_Rpp29_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023538">
        <property type="entry name" value="RNP1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023534">
        <property type="entry name" value="Rof/RNase_P-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002730">
        <property type="entry name" value="Rpp29/RNP1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF046110">
        <property type="entry name" value="RNaseP1Mthb"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13348">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01868">
        <property type="entry name" value="RNase_P-MRP_p29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00538">
        <property type="entry name" value="POP4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101744">
        <property type="entry name" value="Rof/RNase P subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 7 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006181"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96176.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="94" mass="10829" checksum="EF78E08FEF16AB79" modified="2013-06-26" version="1">MNPKDILRHELIGLKVEIVHAKNKSMIGIKGKVVDETRKTLIIEKDSGKEVVIPKDIAIFLFHLDNCKVKVDGRLLIGRPEERLKKKIKILYPY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSS9</accession>
    <name>N6VSS9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Histidinol-phosphate aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Imidazole acetol-phosphate transaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">hisC</name>
        <name evidence="9" type="ORF">J422_03308</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>L-histidinol phosphate + 2-oxoglutarate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:23744"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57766"/>
            <dbReference type="ChEBI" id="CHEBI:57980"/>
            <dbReference type="EC" id="2.6.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 7/9.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. Histidinol-phosphate aminotransferase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.9" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96256.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590958.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSS9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01048"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.648"/>
    <dbReference type="OrthoDB" id="9929at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00012"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004400">
        <property type="term" value="F:histidinol-phosphate transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01023">
        <property type="entry name" value="HisC_aminotrans_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005861">
        <property type="entry name" value="HisP_aminotrans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01141">
        <property type="entry name" value="hisC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885:SF2">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42885">
        <property type="entry name" value="HISTIDINOL-PHOSPHATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 7 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6 7" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="5 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="29"/>
            <end position="353"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="7">
        <location>
            <position position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96256.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="365" mass="41873" checksum="2EA08AD2831B484A" modified="2013-06-26" version="1">MIREVVKKLKPYVPGKSIEELKRMYGLKEIIKLGSNENPWGCSPKVKEAILKEINNIHHYPEPINPILMEELSMFLNVDKNNIIVGGDGADEVIDTLFKTFVDKGDEVIIPIPTFTQYRVSAQIYDAKIKFAKFDKKNGFKLDVESVLNNITNRTKLIFLCSPNNPTGNVIENRDIERILKETEAIVVIDHAYIEYADKKYDWSRKVLKYNNAIVLRTFSKVFGLAGMRIGYGIANEEIINYMMRVKPVFSLTRLSQVAAIEALRDKEFLDFSIKNGIKSREMLYSGLKRLGIKVYPSQANYVLAEIENGKMVYEELLKRGVIVRSCSSFDGLDERYLRISIGKFEEIEKFLSILEGILYDIGNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VST4</accession>
    <name>N6VST4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Diadenylate cyclase</fullName>
            <shortName evidence="6">DAC</shortName>
            <ecNumber evidence="6">2.7.7.85</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Cyclic-di-AMP synthase</fullName>
            <shortName evidence="6">c-di-AMP synthase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">dacZ</name>
        <name evidence="8" type="ORF">J422_03333</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Diadenylate cyclase that catalyzes the condensation of 2 ATP molecules into cyclic di-AMP (c-di-AMP). c-di-AMP is a second messenger for intracellular signal transduction involved in the control of important regulatory processes such as osmoregulation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 6">
            <text>2 ATP = 3',3'-c-di-AMP + 2 diphosphate</text>
            <dbReference type="Rhea" id="RHEA:35655"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:71500"/>
            <dbReference type="EC" id="2.7.7.85"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the adenylate cyclase family. DacZ subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.85" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96261.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590969.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VST4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01053"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.653"/>
    <dbReference type="OrthoDB" id="85944at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004016">
        <property type="term" value="F:adenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106408">
        <property type="term" value="F:diadenylate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1700.10">
        <property type="entry name" value="DNA integrity scanning protein, DisA, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00840">
        <property type="entry name" value="DacZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014499">
        <property type="entry name" value="DAC_DacZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050338">
        <property type="entry name" value="DisA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036888">
        <property type="entry name" value="DNA_integrity_DisA_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003390">
        <property type="entry name" value="DNA_integrity_scan_DisA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34185">
        <property type="entry name" value="DIADENYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34185:SF1">
        <property type="entry name" value="DIADENYLATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02457">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019073">
        <property type="entry name" value="UCP019073"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143597">
        <property type="entry name" value="YojJ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51794">
        <property type="entry name" value="DAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0464">Manganese</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 6" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DAC" evidence="7">
        <location>
            <begin position="138"/>
            <end position="295"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51794"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96261.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="303" mass="33898" checksum="13E9D9E7AB769A81" modified="2013-06-26" version="1">MISKYIIKHGIELAYDIKAKAFLLFTESGKSYEILKSIKVEKSSKSIFKFLNIDKLKIIVITPNISTYNKLKNEKDIIPLYVRYKEENRQMIITSGVLQAIEQDLLKEKDTVVAVLGDPKLKMLDTIMVINIREHIKSLKIYKLLETLDEKLRKTFKEVLKLAIEIGREGREGKQVGTIFVIGDTLNVMNNSKPLILNPFAGHNAKIFDKDVKGTIKELCSIDGAFIITDDGKVVSAGRYLETKGNVKLPSGLGARHMAAADITKNTNAIAITVSQSGGIVRVFKDGEIVFELDPKKNAILFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT07</accession>
    <name>N6VT07_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Cell division protein FtsY</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_02584</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
            <orientation evidence="1">Cytoplasmic side</orientation>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the GTP-binding SRP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96331.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590542.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT07"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00069"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.506"/>
    <dbReference type="OrthoDB" id="372188at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005047">
        <property type="term" value="F:signal recognition particle binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006614">
        <property type="term" value="P:SRP-dependent cotranslational protein targeting to membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd17874">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000053">
        <property type="entry name" value="Signal recognition particle receptor FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.140">
        <property type="entry name" value="Signal recognition particle SRP54, nucleotide-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00920">
        <property type="entry name" value="FtsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013822">
        <property type="entry name" value="Signal_recog_particl_SRP54_hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004390">
        <property type="entry name" value="SR_rcpt_FtsY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036225">
        <property type="entry name" value="SRP/SRP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000897">
        <property type="entry name" value="SRP54_GTPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042101">
        <property type="entry name" value="SRP54_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00064">
        <property type="entry name" value="ftsY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43134:SF1">
        <property type="entry name" value="SIGNAL RECOGNITION PARTICLE RECEPTOR SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00448">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02881">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00962">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00963">
        <property type="entry name" value="SRP54_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47364">
        <property type="entry name" value="Domain of the SRP/SRP receptor G-proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00300">
        <property type="entry name" value="SRP54"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="11" id="KW-0132">Cell division</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-0675">Receptor</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SRP54-type proteins GTP-binding" evidence="10">
        <location>
            <begin position="258"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="11">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004413"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023170"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96331.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="297" mass="32907" checksum="ED65D2F525208A3A" modified="2013-06-26" version="1" fragment="single">KKEIVIVEEDIEDILEELRIELLEADVAYDVVEKLIENIKNELIGKKITPEDNIEEITLMAIKNAIKNILSQKKIDIYKIIEENRKKGKPTVIVFVGINGTGKTTTIAKLAYKLKNKGYSVVLAAGDTFRAGAIEQLEKHGKNIGVRVIKHKHGADAAAVIYDAIEHAKAKKIDVVLADTAGRQATNINLMDEMKKIVRVTKPDLVIFVGDALTGNDAIYQAEEFDKAVGIDGVILTKVDADAKGGAALSIGYAIGKPILFLGVGQRYEDLIEFDPEWFVKKLFGEEEIRFSTEREF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT12</accession>
    <name>N6VT12_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Mechanosensitive ion channel MscS</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_02609</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the MscS (TC 1.A.23) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96336.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590551.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT12"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00074"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.511"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1260">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010920">
        <property type="entry name" value="LSM_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023408">
        <property type="entry name" value="MscS_beta-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006685">
        <property type="entry name" value="MscS_channel_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011014">
        <property type="entry name" value="MscS_channel_TM-2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30566:SF5">
        <property type="entry name" value="MECHANOSENSITIVE ION CHANNEL PROTEIN 1, MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30566">
        <property type="entry name" value="YNAI-RELATED MECHANOSENSITIVE ION CHANNEL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00924">
        <property type="entry name" value="MS_channel_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82861">
        <property type="entry name" value="Mechanosensitive channel protein MscS (YggB), transmembrane region"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50182">
        <property type="entry name" value="Sm-like ribonucleoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="85"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="139"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="domain" description="Mechanosensitive ion channel MscS" evidence="7">
        <location>
            <begin position="184"/>
            <end position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00924"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96336.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="251" mass="28256" checksum="A750D61C3D424CC8" modified="2013-06-26" version="1">MVDMGYYLNILVNLAIGLIICIVIIVLGFILASIVSSMFKKYLRKWAEKTETKIDDIIIEAIESPIKIIIIVYAIYFGLSFLPLPYIITLAIGNFVKFVVILAITWFVAKIGEGSLEHYILPYIKKEIYEGLDENQSKLFKNLTTITICIIGLLIALDSVGFDISILLLLIAIFGLGFALALKDIFSHFLSGVLLFWNKIMKVNNNIKVGSVIGRVKEMHLTYTKLETPEGEEVIIPNAKLINEIIIKKKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT41</accession>
    <name>N6VT41_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Nickel-transporting ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_02744</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96361.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590602.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT41"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00100"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.538"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0055085">
        <property type="term" value="P:transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050319">
        <property type="entry name" value="ABC_transp_ATP-bind"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776:SF7">
        <property type="entry name" value="D,D-DIPEPTIDE TRANSPORT ATP-BINDING PROTEIN DDPF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43776">
        <property type="entry name" value="TRANSPORT ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="2"/>
            <end position="245"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96361.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="293" mass="34099" checksum="CEC6D8C445A6B42E" modified="2013-06-26" version="1">MIIGKNLTKIFYHGYIRKTKIVAVNNVSLKINENETVCLIGESGSGKSTLGHLLLRLIKPDKGDIYFYNSNITKMKEKEIRKIRKYLQLIPQHPDLSLNPRWKIKKSLLEPLILNKIENKDEVLRRVLYDVGIKEELLDRYPHEVSGGELQRVIIARAISLKPKFIVCDEPTSMLDISTQASILNLLKKIKEEYKLSYLFITHDLDVAKIMGDKFLVMYRGEIIEENDNLEKPLHPYTEMLLNRKRLLENNGNVNNGCKFFYHCPYAEEICFKEEPPTVEVNGKKVKCFKYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT72</accession>
    <name>N6VT72_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Pyridine nucleotide-disulfide oxidoreductase dimerization region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_02414</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96396.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590424.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT72"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00788"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.472"/>
    <dbReference type="OrthoDB" id="27922at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050260">
        <property type="entry name" value="FAD-bd_OxRdtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016156">
        <property type="entry name" value="FAD/NAD-linked_Rdtase_dimer_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004099">
        <property type="entry name" value="Pyr_nucl-diS_OxRdtase_dimer"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429:SF3">
        <property type="entry name" value="NITRITE REDUCTASE [NAD(P)H]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43429">
        <property type="entry name" value="PYRIDINE NUCLEOTIDE-DISULFIDE OXIDOREDUCTASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02852">
        <property type="entry name" value="Pyr_redox_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00411">
        <property type="entry name" value="PNDRDTASEI"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55424">
        <property type="entry name" value="FAD/NAD-linked reductases, dimerisation (C-terminal) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0274">FAD</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="6">
        <location>
            <begin position="1"/>
            <end position="288"/>
        </location>
    </feature>
    <feature type="domain" description="Pyridine nucleotide-disulphide oxidoreductase dimerisation" evidence="5">
        <location>
            <begin position="326"/>
            <end position="423"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001974"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96396.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="443" mass="48298" checksum="D94D8D16A1234DD7" modified="2013-06-26" version="1">MRVVIVGSGAAGLTAASTVRKYDKEKEIIVITKERDIAYSPCAIPYVIEGTIKSFEDIIMHKPEDYKKRKNIDVLTETEVIDIDSRGNKVVLDNGEKIDYDYLVLSTGSTPFIPPIKGVNLDGVFTVKTIDDGRRILKYIEENNCKRAVIAGAGAIGLEMGYALKKRGLDVLIVEMAPQILPRFLDPDMAEIVQKYLESEGIRFILSKPLEMIIGDDKVKGVVVGGKEFDADIVILSTGVRPNIDLAKKAGCKVDKAIVVDEYMRTSIKNIYAAGDCVEVVDFITGEKTLSPFGSTAVRQGKVVGLNIVGKNVEFPPVLNSAVSKIGSLEIGGTGLTAFSANLKRIPIVIGKAKALTRARYYPGGKDIYVKLVFGEDMKILGCQIIGGERVAERIDLVSLAIYNGVKAEELCKMEHCYAPPVAMVNEPISLAAEEALNYFKNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT91</accession>
    <name>N6VT91_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein uS3</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rps3p</name>
        <name evidence="6" type="synonym">rps3</name>
        <name evidence="10" type="ORF">J422_02544</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Binds the lower part of the 30S subunit head.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8">Belongs to the universal ribosomal protein uS3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96416.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590465.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT91"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00764"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.498"/>
    <dbReference type="OrthoDB" id="9126at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02411">
        <property type="entry name" value="KH-II_30S_S3_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.300.20:FF:000001">
        <property type="entry name" value="30S ribosomal protein S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1140.32">
        <property type="entry name" value="Ribosomal protein S3, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01309_A">
        <property type="entry name" value="Ribosomal_uS3_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036419">
        <property type="entry name" value="Ribosomal_S3_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057258">
        <property type="entry name" value="Ribosomal_uS3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027488">
        <property type="entry name" value="Ribosomal_uS3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001351">
        <property type="entry name" value="Ribosomal_uS3_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018280">
        <property type="entry name" value="Ribosomal_uS3_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005703">
        <property type="entry name" value="Ribosomal_uS3_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003219">
        <property type="entry name" value="PRK04191.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01008">
        <property type="entry name" value="uS3_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11760:SF32">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN US3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00189">
        <property type="entry name" value="Ribosomal_S3_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54821">
        <property type="entry name" value="Ribosomal protein S3 C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50823">
        <property type="entry name" value="KH_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00548">
        <property type="entry name" value="RIBOSOMAL_S3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="KH type-2" evidence="9">
        <location>
            <begin position="16"/>
            <end position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01309"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50823"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96416.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="203" mass="22727" checksum="7407CF7D89849F82" modified="2013-06-26" version="1">MIERVFVDENKKKVLIDEYFKKELIRAGYSHCEIRKTPIGTKITIYAEKPGLVIGRRGAKIRELTDTLAKEFNVEKPQIDVKPIENPDLDAQVVAEKIARALERGLHFRRVGHTAVRRVMNAGAKGVLVVISGKLTGERARTEKFMAGYMKHCGEPAAELVDEGRAIAMTKPGVIGVTVKIMRPDVLLPDEIIIKEDAEKEKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTN0</accession>
    <name>N6VTN0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Methionine synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_01895</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96526.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTN0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00227"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.373"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003871">
        <property type="term" value="F:5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009086">
        <property type="term" value="P:methionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03311">
        <property type="entry name" value="CIMS_C_terminal_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002629">
        <property type="entry name" value="Met_Synth_C/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038071">
        <property type="entry name" value="UROD/MetE-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002119">
        <property type="entry name" value="PRK00957.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30519">
        <property type="entry name" value="5-METHYLTETRAHYDROPTEROYLTRIGLUTAMATE--HOMOCYSTEINE METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01717">
        <property type="entry name" value="Meth_synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51726">
        <property type="entry name" value="UROD/MetE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Cobalamin-independent methionine synthase MetE C-terminal/archaeal" evidence="4">
        <location>
            <begin position="1"/>
            <end position="265"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01717"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96526.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="271" mass="30827" checksum="105EDA10EF27F266" modified="2013-06-26" version="1">MITTVVGSYPVVFKENEDFFDKIKKIFGIYDPYKYAIEVAVKDQINAGIDIISDGQVRGDMVEIFTSKLFGFEDRRVVNRVEFVEPITLKDILYAKKIAKKLNSNVEVKGIITGPNTIAYSVKDEYYSNKEELIFDIAKALRNEVLAIKNHVSYIQIDEPILSVGIADFEVAKRAIDIIVRGINKSFGMHVCGKVYNIIDELNEFNVSILDHEFASNRKNLEILDRIKKKVGFGCVNTKVNKVESVEEIKGLIKEGLEIIKNNNFISEKIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTX5</accession>
    <name>N6VTX5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Dihydroneopterin aldolase</fullName>
            <shortName evidence="1">DHNA</shortName>
            <ecNumber evidence="1">4.1.2.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">7,8-dihydroneopterin aldolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">mptD</name>
        <name evidence="3" type="ORF">J422_01228</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the conversion of 7,8-dihydroneopterin (H2Neo) to 6-hydroxymethyl-7,8-dihydropterin (6-HMD).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>7,8-dihydroneopterin = 6-hydroxymethyl-7,8-dihydropterin + glycolaldehyde</text>
            <dbReference type="Rhea" id="RHEA:10540"/>
            <dbReference type="ChEBI" id="CHEBI:17001"/>
            <dbReference type="ChEBI" id="CHEBI:17071"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="EC" id="4.1.2.25"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the archaeal dihydroneopterin aldolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.2.25" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589911.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTX5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00043"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.240"/>
    <dbReference type="OrthoDB" id="132689at2157"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004150">
        <property type="term" value="F:dihydroneopterin aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001118">
        <property type="term" value="P:tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1300.20">
        <property type="entry name" value="7,8-dihydroneopterin aldolase (MptD)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02130">
        <property type="entry name" value="DHNA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027508">
        <property type="entry name" value="DHN_aldolase_MptD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036839">
        <property type="entry name" value="MptD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007181">
        <property type="entry name" value="MtpD_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04038">
        <property type="entry name" value="DHNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143560">
        <property type="entry name" value="MK0786-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0456">Lyase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroneopterin aldolase MtpD C-terminal" evidence="2">
        <location>
            <begin position="18"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="114"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02130"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="121" mass="14281" checksum="6614C93522E66CEF" modified="2013-06-26" version="1">MRVEEEELFNKYFKNLSQRERAIFEGGIKLGAIFHQFIGTPVSKKNKDLLERAIEESIKNQPFVYDIKVKIKNVSERYKALSEDMLDVDLIIKVSNVKAYLKLEYVKELSYPLMYVKKVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTY6</accession>
    <name>N6VTY6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Phosphomannomutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_01041</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96636.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589876.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTY6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00836"/>
    <dbReference type="OrthoDB" id="10363at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016868">
        <property type="term" value="F:intramolecular phosphotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03089">
        <property type="entry name" value="PMM_PGM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771:SF1">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="9">
        <location>
            <begin position="2"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="10">
        <location>
            <begin position="150"/>
            <end position="245"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="11">
        <location>
            <begin position="251"/>
            <end position="360"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="8">
        <location>
            <begin position="365"/>
            <end position="427"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="12">
        <location>
            <position position="428"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96636.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="428" mass="49492" checksum="1FC32FCE78F0BEC5" modified="2013-06-26" version="1" fragment="single">MVFKAYDIRGIYGKEIDERFAYSLGKCLGEKFKDKDVLVGNDVRFGSKNLLPYFILGLSENANVHYAGLISTPLLYFGTKGKYDLGVIITASHNPPEYTGFKMCDKKAIPLSPKEEIKPIFKFYDIDRSVKEEAEKLDLDNYKVDVIEEYKKFFMKKFDKDFDIKIAVDFANGATTVAEKEILKELFDDIVLINDYPDGSFPAHLPDTLKEECLKDIKKAVFENKCDLGLIFDGDGDRLGMVDEREETLRGDIITAIIAKEILKENKGVKIIYDLRCSKIVPEIIEKFGGVAIKSRVGHYFIKKLMHEIDGYFAGELSNHFYFKEIGYFESPLLALYYILKIMDEEDKKLSELNREFNKYYHSGEINFKVSDQKKVLEKIKEIYKNCKIEELDGVSVYCKDFWFNLRPSNTEPLIRLNLEAEKEEILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VWX5</accession>
    <name>N6VWX5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06977</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000066">
        <property type="protein sequence ID" value="ENN95597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594664.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VWX5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01230"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1351"/>
    <dbReference type="OrthoDB" id="65038at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="139" mass="15774" checksum="7662E6072EFA923E" modified="2013-06-26" version="1">MINIIDYPYKKLTIEHNNGLILIKLRSKDDEDYIKMNLEAASLLRLFLDKSVIIAERMTYNFKKNNMKTDKITDIGTVFGINGRMSVGVLPADCERIACILLGLHKENKDMSIVLKPKKAALMSLIISKIVADNYLKSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX67</accession>
    <name>N6VX67_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06461</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95707.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593548.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX67"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01283"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1258"/>
    <dbReference type="OrthoDB" id="66072at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="3"/>
            <end position="56"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95707.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="81" mass="9794" checksum="17A1236F0323B081" modified="2013-06-26" version="1">MEKIIIKSMKKVFNEELKNLEEELNNILKKYEVRSVRELKEKLANSKIKVEETDLKRAEELEKYIERVMKCLREINIKAIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXT0</accession>
    <name>N6VXT0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">RCK N-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04875</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95947.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592065.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXT0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00516"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.955"/>
    <dbReference type="OrthoDB" id="350705at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006813">
        <property type="term" value="P:potassium ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1640.10">
        <property type="entry name" value="inorganic pyrophosphatase (n-terminal core)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001667">
        <property type="entry name" value="DDH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038763">
        <property type="entry name" value="DHH_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003156">
        <property type="entry name" value="DHHA1_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051319">
        <property type="entry name" value="Oligoribo/pAp-PDE_c-di-AMP_PDE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003148">
        <property type="entry name" value="RCK_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47618">
        <property type="entry name" value="BIFUNCTIONAL OLIGORIBONUCLEASE AND PAP PHOSPHATASE NRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47618:SF1">
        <property type="entry name" value="BIFUNCTIONAL OLIGORIBONUCLEASE AND PAP PHOSPHATASE NRNA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01368">
        <property type="entry name" value="DHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02272">
        <property type="entry name" value="DHHA1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02254">
        <property type="entry name" value="TrkA_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64182">
        <property type="entry name" value="DHH phosphoesterases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51201">
        <property type="entry name" value="RCK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RCK N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95947.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="463" mass="51294" checksum="6FBB831D76304434" modified="2013-06-26" version="1">MIVVIGFGSFGRKVVNFIKNKEPITIIDKQIEDIDDLIKENIKVVVGDATEEETLKKANIENADIVLILTNSEVNRNIAEKVCELSPNSYKIARAVQGYPELYSGLNIDKIVNILESGAKDIANEVDNAKLKRKLMKLKSIILEKKKKCNGKKMLILTHINPDPDAIASAMALKTICEKWDVEADIGYGGNIGYDENKTMVNLLEVNLLSIDQINIDDYCIIAAVDFSSSKQLPMEVEKLDIIIDHHANGDLEADYKDILNVGATSSILTNYLKELNMEPSRKLATALFYGIESDTNNFKRGVTRFDFECAAYLQSYIDTTILEMIENPEISTEIMEVIAKAIINRQVVKGNIALSYVGEITNRDALPKAADFLLKMEGISTTFVFGIVGDYIEISARTKDVRLNLGEILKKAFGGGGHQTAAGARIELGIFKAVSDKEALRKLVEEAIKAKILEVIGIKEKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXU2</accession>
    <name>N6VXU2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Dihydroorotate dehydrogenase</fullName>
            <shortName evidence="10">DHOD</shortName>
            <shortName evidence="10">DHODase</shortName>
            <shortName evidence="10">DHOdehase</shortName>
            <ecNumber evidence="10">1.3.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pyrD</name>
        <name evidence="12" type="ORF">J422_04925</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the conversion of dihydroorotate to orotate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>(S)-dihydroorotate + A = orotate + AH2</text>
            <dbReference type="Rhea" id="RHEA:18073"/>
            <dbReference type="ChEBI" id="CHEBI:13193"/>
            <dbReference type="ChEBI" id="CHEBI:17499"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:30864"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="10">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Pyrimidine metabolism; UMP biosynthesis via de novo pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Heterotetramer of 2 PyrK and 2 PyrD type B subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.-.-" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95957.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592085.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXU2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00506"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.965"/>
    <dbReference type="OrthoDB" id="36608at2157"/>
    <dbReference type="UniPathway" id="UPA00070"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004152">
        <property type="term" value="F:dihydroorotate dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04740">
        <property type="entry name" value="DHOD_1B_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000027">
        <property type="entry name" value="Dihydropyrimidine dehydrogenase [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.26.10">
        <property type="entry name" value="Dihydroorotate Dehydrogenase A, chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00224">
        <property type="entry name" value="DHO_dh_type1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050074">
        <property type="entry name" value="DHO_dehydrogenase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033888">
        <property type="entry name" value="DHOD_1B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023359">
        <property type="entry name" value="Dihydro_DH_chainA_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024920">
        <property type="entry name" value="Dihydroorotate_DH_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012135">
        <property type="entry name" value="Dihydroorotate_DH_1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005720">
        <property type="entry name" value="Dihydroorotate_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001295">
        <property type="entry name" value="Dihydroorotate_DH_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049622">
        <property type="entry name" value="Dihydroorotate_DH_I"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005574">
        <property type="entry name" value="PRK07259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01037">
        <property type="entry name" value="pyrD_sub1_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109:SF1">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (FUMARATE)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48109">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE (QUINONE), MITOCHONDRIAL-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01180">
        <property type="entry name" value="DHO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000164">
        <property type="entry name" value="DHO_oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51395">
        <property type="entry name" value="FMN-linked oxidoreductases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00911">
        <property type="entry name" value="DHODEHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00912">
        <property type="entry name" value="DHODEHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7 10" id="KW-0288">FMN</keyword>
    <keyword evidence="9 10 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 10" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Dihydroorotate dehydrogenase catalytic" evidence="11">
        <location>
            <begin position="2"/>
            <end position="282"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="10">
        <location>
            <position position="128"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="18"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="42"/>
            <end position="43"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="66"/>
            <end position="70"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="187"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="188"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="238"/>
            <end position="239"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="260"/>
            <end position="261"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00008008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01180"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95957.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="298" mass="32590" checksum="CE52B499BD85F863" modified="2013-06-26" version="1">MEIKLCGIKFKNPIFLASGIMGDNAGALKRIAINGAGAVTTKSIGLKPNMGHKNPVIVELDEGFLNAVGLANPGVDNYIEELKKIRGFLDKIGVRIIASIYGKDEEEFKEVAKKIEPYVDMIELNISCPHAKGYGLEIGQDVDLSYNVCKSVKSAVNIPVFAKISPMVNIREIAEALCNVVDGFTAINTVKGMVIDINMKKPILSNKFGGLSGKYIKPIGVRVVYELYEYKKPIIGVGGIFNGRDALEYFMAGASAVQIGSAVYFRGYDVFNKIYKEIKEFLDKEGLELKDIIGIAHE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXV3</accession>
    <name>N6VXV3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit Rpo10</fullName>
            <ecNumber evidence="6">2.7.7.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">DNA-directed RNA polymerase subunit N</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpo10</name>
        <name evidence="6" type="synonym">rpoN</name>
        <name evidence="7" type="ORF">J422_04975</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the archaeal Rpo10/eukaryotic RPB10 RNA polymerase subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95967.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592105.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXV3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00495"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.975"/>
    <dbReference type="OrthoDB" id="371754at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00250">
        <property type="entry name" value="RNApol_arch_Rpo10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023580">
        <property type="entry name" value="RNA_pol_su_RPB10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020789">
        <property type="entry name" value="RNA_pol_suN_Zn-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000268">
        <property type="entry name" value="RPABC5/Rpb10"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003089">
        <property type="entry name" value="PRK04016.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431:SF3">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23431">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC5 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01194">
        <property type="entry name" value="RNA_pol_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005653">
        <property type="entry name" value="RNA_pol_N/8_sub"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46924">
        <property type="entry name" value="RNA polymerase subunit RPB10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01112">
        <property type="entry name" value="RNA_POL_N_8KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1 6 7" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 6 7" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 6" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95967.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="73" mass="8716" checksum="96B966D14F3A88D8" modified="2013-06-26" version="1">MMFPIRCFSCGKVIGEVFEEYKRRILNGEHPKDVLDDLGIKKYCCRRMFISYRISKDGKEIFDEIIEHDIARL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYI2</accession>
    <name>N6VYI2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Small ribosomal subunit protein uS14</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rps14P</name>
        <name evidence="7" type="synonym">rps14</name>
        <name evidence="8" type="ORF">J422_03978</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Binds 16S rRNA, required for the assembly of 30S particles.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="7">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the universal ribosomal protein uS14 family. Zinc-binding uS14 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96182.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591410.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYI2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00425"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.780"/>
    <dbReference type="OrthoDB" id="5615at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.830.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.830.10">
        <property type="entry name" value="30s Ribosomal Protein S14, Chain N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01364_A">
        <property type="entry name" value="Ribosomal_uS14_2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001209">
        <property type="entry name" value="Ribosomal_uS14"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023676">
        <property type="entry name" value="Ribosomal_uS14_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018271">
        <property type="entry name" value="Ribosomal_uS14_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039744">
        <property type="entry name" value="RIbosomal_uS14_euk_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043140">
        <property type="entry name" value="Ribosomal_uS14_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004424">
        <property type="entry name" value="PRK05766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12010:SF2">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00253">
        <property type="entry name" value="Ribosomal_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00527">
        <property type="entry name" value="RIBOSOMAL_S14"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="17"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="20"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96182.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="53" mass="6215" checksum="6FFEEC4D22FB59FC" modified="2013-06-26" version="1">MAKKPWKKKYGYGVRPCERCGHVGFGLIRKYGLNLCRQCFREVAHKLGFKKLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYV1</accession>
    <name>N6VYV1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03069</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96307.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590758.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYV1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00161"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.602"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96307.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="74" mass="8820" checksum="E2DCFC7FE70F8000" modified="2013-06-26" version="1">MKKVEEVIRDIHLINRRARFEGIKIFMTKNILKKCKEKGILDEVLISTKNTEIEDLVRKSYLFMDENSVCKKTF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYX7</accession>
    <name>N6VYX7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chorismate lyase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02589</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96332.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590544.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYX7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00070"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.507"/>
    <dbReference type="OrthoDB" id="145449at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1410.10">
        <property type="entry name" value="Chorismate lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028978">
        <property type="entry name" value="Chorismate_lyase_/UTRA_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002800">
        <property type="entry name" value="Rv2949c-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01947">
        <property type="entry name" value="Rv2949c-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64288">
        <property type="entry name" value="Chorismate lyase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96332.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="164" mass="19196" checksum="BE8A80A15E35E263" modified="2013-06-26" version="1">MKIYEKISELNKKFPLLNEEKILLGTDGSVTNILEILFEGECVVKTLSQKVINNVNYREVILSVKEPLVYAVSKTPFKNIEYGLREEIKRDLLSADIPIGKILRKYNLETRREIINIDIAKLDNRLKTLLNTEYDELPKRTYHIIYKNRILMIITEIFAIRGKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ53</accession>
    <name>N6VZ53_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">anthranilate synthase</fullName>
            <ecNumber evidence="3">4.1.3.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Anthranilate synthase, GATase component</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">J422_02489</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of a heterotetrameric complex that catalyzes the two-step biosynthesis of anthranilate, an intermediate in the biosynthesis of L-tryptophan. In the first step, the glutamine-binding beta subunit (TrpG) of anthranilate synthase (AS) provides the glutamine amidotransferase activity which generates ammonia as a substrate that, along with chorismate, is used in the second step, catalyzed by the large alpha subunit of AS (TrpE) to produce anthranilate. In the absence of TrpG, TrpE can synthesize anthranilate directly from chorismate and high concentrations of ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21732"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="4.1.3.27"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer consisting of two non-identical subunits: a beta subunit (TrpG) and a large alpha subunit (TrpE).</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.3.27" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96407.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ53"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00775"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.487"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004049">
        <property type="term" value="F:anthranilate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01743">
        <property type="entry name" value="GATase1_Anthranilate_Synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.880:FF:000003">
        <property type="entry name" value="Anthranilate synthase component II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006221">
        <property type="entry name" value="TrpG/PapA_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00566">
        <property type="entry name" value="trpG_papA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF4">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="6 13" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glutamine amidotransferase" evidence="12">
        <location>
            <begin position="15"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004873"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011575"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00025634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00079115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96407.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="203" mass="23095" checksum="F86C39014C52358E" modified="2013-06-26" version="1">MYWKIIYKVIKMKVLIIDNIDSFVWNLVQYVGTLGHKVILRDNKITVNDIKKLDPDCIIISPGPKRPKDAGNCIDIILNVDLPILGVCLGHQCIVEAFGGRVDRAERVMHGKASLIEHDGKNIYRNLPKRFYGGRYHSLIAKEVPNCLKITAVSLDDNYIMGVRHKKLPIEGVQFHPESILTESDELKFPYLGLKMIKNFIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZH5</accession>
    <name>N6VZH5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Carbamoyl phosphate synthase small chain</fullName>
            <ecNumber evidence="11">6.3.5.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Carbamoyl phosphate synthetase glutamine chain</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">carA</name>
        <name evidence="13" type="ORF">J422_01805</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Small subunit of the glutamine-dependent carbamoyl phosphate synthetase (CPSase). CPSase catalyzes the formation of carbamoyl phosphate from the ammonia moiety of glutamine, carbonate, and phosphate donated by ATP, constituting the first step of 2 biosynthetic pathways, one leading to arginine and/or urea and the other to pyrimidine nucleotides. The small subunit (glutamine amidotransferase) binds and cleaves glutamine to supply the large subunit with the substrate ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>hydrogencarbonate + L-glutamine + 2 ATP + H2O = carbamoyl phosphate + L-glutamate + 2 ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:18633"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.</text>
    </comment>
    <comment type="pathway">
        <text evidence="11">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate. Tetramer of heterodimers (alpha,beta)4.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the CarA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.5" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96512.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590118.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZH5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00211"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.355"/>
    <dbReference type="OrthoDB" id="7675at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00171"/>
    </dbReference>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00115"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004088">
        <property type="term" value="F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006541">
        <property type="term" value="P:glutamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01744">
        <property type="entry name" value="GATase1_CPSase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.20">
        <property type="entry name" value="Carbamoyl-phosphate synthase small subunit, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01209">
        <property type="entry name" value="CPSase_S_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050472">
        <property type="entry name" value="Anth_synth/Amidotransfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006274">
        <property type="entry name" value="CarbamoylP_synth_ssu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002474">
        <property type="entry name" value="CarbamoylP_synth_ssu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036480">
        <property type="entry name" value="CarbP_synth_ssu_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035686">
        <property type="entry name" value="CPSase_GATase1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017926">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01368">
        <property type="entry name" value="CPSaseIIsmall"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009475">
        <property type="entry name" value="PRK12838.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418:SF7">
        <property type="entry name" value="CARBAMOYL-PHOSPHATE SYNTHASE SMALL CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43418">
        <property type="entry name" value="MULTIFUNCTIONAL TRYPTOPHAN BIOSYNTHESIS PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00988">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00117">
        <property type="entry name" value="GATase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00097">
        <property type="entry name" value="ANTSNTHASEII"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00099">
        <property type="entry name" value="CPSGATASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00096">
        <property type="entry name" value="GATASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01097">
        <property type="entry name" value="CPSase_sm_chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52021">
        <property type="entry name" value="Carbamoyl phosphate synthetase, small subunit N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 11" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8 11" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carbamoyl-phosphate synthase small subunit N-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="region of interest" description="CPSase" evidence="11">
        <location>
            <begin position="1"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="324"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="221"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="247"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="290"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="291"/>
        </location>
        <ligand>
            <name>L-glutamine</name>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007800"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96512.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="347" mass="38915" checksum="C977B9D26D32B5AE" modified="2013-06-26" version="1">MEAVLVLEDGTVLKGYGFGAEKEVYGELVFTTVMTGYVEVLTDPSYKGQIVVMTYPLIGNYGVSERWFESDGIKVEGYVVREATSDSIKEFLKSYDIPGIEGIDTRFLTKKIRSGGVVKACLKTGEKIGKDEIEEIKEKVKDYKDISEIDLVPLVSTKKVIIHKPDKVKYRCVLIDCGVKKNIINNLLKRNCEVIQVPYNTSYDKILEYKPDFVLISNGPGDPKRLKEVIKTIKCLFGHLPLTGICLGNQLLALALGGDTYKMKFGHRGGNHPVKDLRDGKVYITSQNHGFAVKDVDADIIFKNLNDNTVEGIADKDILSVQFHPEARPGPRDTEFIFDQMVKLIRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZJ1</accession>
    <name>N6VZJ1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Exosortase EpsH-related protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_01900</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96527.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590138.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZJ1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00228"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.374"/>
    <dbReference type="OrthoDB" id="66119at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008233">
        <property type="term" value="F:peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026432">
        <property type="entry name" value="Archaeo_ArtD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026392">
        <property type="entry name" value="Exo/Archaeosortase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04175">
        <property type="entry name" value="archaeo_artD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04178">
        <property type="entry name" value="exo_archaeo"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-0645">Protease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="35"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="55"/>
            <end position="75"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="87"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="121"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96527.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="11">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="149" mass="17445" checksum="FB435DD071CFA4B2" modified="2013-06-26" version="1">MRKDYVKNIAFVFIISYSILSILENFLSNILSSIIGHLMHIYTYNNLLFFNNKIFIIDRACTCSLEMTIFLSFVFATPKVKITYKILYSIFGLLVINVVNILRIIMIITYKTDNFQLAHDVISFILFPVALFLNVFWIVILKKLNILRC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZN4</accession>
    <name>N6VZN4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">4Fe-4S ferredoxin iron-sulfur binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_01645</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000012">
        <property type="protein sequence ID" value="ENN96557.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590019.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000012.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZN4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00324"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.324"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF2">
        <property type="entry name" value="FERREDOXIN 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12837">
        <property type="entry name" value="Fer4_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="74"/>
            <end position="103"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96557.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="137" mass="16548" checksum="1B28D5173C4F17EF" modified="2013-06-26" version="1">MEKEKEVLKKVREFMILNLEIKKLLKELNFEDIYEEYERVTKILRKPNIELYRRLYNAVLELEYNGKKRKEIPWFPRIDYDKCKNCKKCVEFCPRGVYDIEDGKVKVKYPYNCIVNCNACAEMCCDNKAIIFPNKTI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZY4</accession>
    <name>N6VZY4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7">Ribosome biogenesis protein Nop10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">nop10</name>
        <name evidence="8" type="ORF">J422_01121</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 7">Involved in ribosome biogenesis; more specifically in 18S rRNA pseudouridylation and in cleavage of pre-rRNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the NOP10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589892.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZY4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00818"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.219"/>
    <dbReference type="OrthoDB" id="7259at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.40">
        <property type="entry name" value="H/ACA ribonucleoprotein complex, subunit Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00803">
        <property type="entry name" value="Nop10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007264">
        <property type="entry name" value="H/ACA_rnp_Nop10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036756">
        <property type="entry name" value="H/ACA_rnp_Nop10_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023532">
        <property type="entry name" value="Nop10_arc-typ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009623">
        <property type="entry name" value="PRK13130.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04135">
        <property type="entry name" value="Nop10p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144210">
        <property type="entry name" value="Nop10-like SnoRNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5 7" id="KW-0698">rRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002325"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009462"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="56" mass="6689" checksum="64D47AD5C2F59EEC" modified="2013-06-26" version="1">MKIRKCPKCGKYTLKDICIYCGEKTFVPKPPKFSLEDKWGKYRRMLKRSLKCKNSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6W004</accession>
    <name>N6W004_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01026</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000006">
        <property type="protein sequence ID" value="ENN96677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589873.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W004"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01013"/>
    <dbReference type="REBASE" id="68594">
        <property type="entry name" value="MviT80McrBCP"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.200"/>
    <dbReference type="OrthoDB" id="9837at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011704">
        <property type="entry name" value="ATPase_dyneun-rel_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052934">
        <property type="entry name" value="Methyl-DNA_Rec/Restrict_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291">
        <property type="entry name" value="5-METHYLCYTOSINE-SPECIFIC RESTRICTION ENZYME B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37291:SF1">
        <property type="entry name" value="TYPE IV METHYL-DIRECTED RESTRICTION ENZYME ECOKMCRB SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07728">
        <property type="entry name" value="AAA_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase dynein-related AAA" evidence="1">
        <location>
            <begin position="805"/>
            <end position="891"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07728"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="1002" mass="118137" checksum="822B8F40BF8E46BF" modified="2013-06-26" version="1">MKKIKKSVKSIDLFKSPILKSYLNSESVNNELIRKLLKEIVLKTNNDNFNNMIDNFGNKIKEILNNSEIKEVGTTILSILATILNYKCYMPIHKKTVSEALRKDININIFWGIHCKNIEDFKQFMKTTNKIREELGIDSMLEVAYYLSKFEGGENMTVKVIENKIPELEKRLRLWKNFVNFVGKRYLPHYDLTSESNWLKIIEKIKNIVEKLEVGEENTEELIKKLFIYQDKDCGWKLIFAGGNVGVFKGFISNAPEEQLKKFVDIIIEVKNSNEFKDEWINKTYNLICEFNRQGVENYGESKLKKQLYNIFGELYGKLHIEREPIMNTCSRNILKEFYDFGEYNYNEFKEAFEMLKQKYKEIVGKLSEFSNGFINLEIDIMFNFFDKTKVWLLAPGKNAKYLEEFYKNNKIFIGWGDIGNLSKQDLNNPDDIKKLIMEKYSEEYNKEPRAAHKMLFDFYKNMNIGDIVVLRRGLKEVVGICKITGDYKYLTTDVPSDYNHSRNVEFIWFSKKGVKLDFSFTQSDTLQKIDNWDTFIKIFEKILNEDSDKVKNSEENIISKELKEKIDKILEKKGQIILYGVPGTGKTYSALKYVEGKGYYKFITFHQSYSYEDFIEGFKPKTTEDGNITYEVEDGIFKRLCILAIWEVLKDKEDIKKEIDLDRLLDKALKEFEDRYPVGSVLKTKMGKEFEIIDYKYEEDRIKSIKIKIESKNEKSLDKSDLEEIFKKALIEGYEIKGPGDIKKIKDTGLNSYYFAMYSELKEIINNIGYIQDENSQYKLSYDEIKEKVIKKLKEHEETKNVFKKEDFQNAKKYYLIIDEINRGNISNILGELITLLEKDKRLSEDNEIIVELPYSKELFAVPPNLYIIGTMNTADRSIALLDIALRRRFGFLEIEPNYDLINKEIEGINLKKLLESLNEKLMKLKDRDHRIGHSYFLNIKDLEDLEFVWYHEIIPLLEEYFYGDVDGLKEVLKDFIEKKENSYEIKRLTGEEFKNAINKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6W0D1</accession>
    <name>N6W0D1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF434 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00255</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589738.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W0D1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01118"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.51"/>
    <dbReference type="OrthoDB" id="60095at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007368">
        <property type="entry name" value="DUF434"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041652">
        <property type="entry name" value="DUF5616"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42252:SF1">
        <property type="entry name" value="DUF434 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42252">
        <property type="entry name" value="DUF5616 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04256">
        <property type="entry name" value="DUF434"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18481">
        <property type="entry name" value="DUF5616"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF434" evidence="1">
        <location>
            <begin position="5"/>
            <end position="60"/>
        </location>
    </feature>
    <feature type="domain" description="DUF5616" evidence="2">
        <location>
            <begin position="68"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04256"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="200" mass="23446" checksum="68455C69578CD2F7" modified="2013-06-26" version="1">MSNKKIEEAKKDFKYLINRGYKKEVAINFVGNHYKLTKLERLRILREVHSDKEIEIVKNKLTPLKELKRVYVDGFNVLITIEAFLKNRVFLCDDGIYRDLERVYGKYKIGEYTEKAIKLLLNVLKDKEFCIYLDAQVSKSGELARRIREKIEKMNLKGKVLCVKNCDSILKTKPAVMTSDLYIILSEDVKHVVDIIKEVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTF9</accession>
    <name>N6UTF9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Histidine triad (HIT) protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_07042</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000068">
        <property type="protein sequence ID" value="ENN95589.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594744.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTF9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00916"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1364"/>
    <dbReference type="OrthoDB" id="26806at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01277">
        <property type="entry name" value="HINT_subgroup"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.428.10">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039384">
        <property type="entry name" value="HINT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019808">
        <property type="entry name" value="Histidine_triad_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001310">
        <property type="entry name" value="Histidine_triad_HIT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011146">
        <property type="entry name" value="HIT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036265">
        <property type="entry name" value="HIT-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648:SF1">
        <property type="entry name" value="ADENOSINE 5'-MONOPHOSPHORAMIDASE HNT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46648">
        <property type="entry name" value="HIT FAMILY PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01230">
        <property type="entry name" value="HIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00332">
        <property type="entry name" value="HISTRIAD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54197">
        <property type="entry name" value="HIT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00892">
        <property type="entry name" value="HIT_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51084">
        <property type="entry name" value="HIT_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HIT" evidence="4">
        <location>
            <begin position="3"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="Histidine triad motif" evidence="2 3">
        <location>
            <begin position="94"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="1">
        <location>
            <position position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601310-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51084"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95589.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="128" mass="14866" checksum="DF8F54429038EA59" modified="2013-06-26" version="1">MCIFCKIANKEIPAKIVYEDDYVVSFLDINPRSKGHTLVIPKRHYKTFDEMDDEYLCHLMKGVKETIKILKKLNFDGYNIVNNNGRAAGQEIEHVHIHIIPRYEGDEAIKFGEVKKIDLDEVYKILIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTQ3</accession>
    <name>N6UTQ3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">ATP-grasp fold PylC-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06306</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000051">
        <property type="protein sequence ID" value="ENN95719.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593435.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000051.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTQ3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01272"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1227"/>
    <dbReference type="OrthoDB" id="11959at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.36.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003806">
        <property type="entry name" value="ATP-grasp_PylC-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016677">
        <property type="entry name" value="UCP016817_carboligase"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02655">
        <property type="entry name" value="ATP-grasp_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016817">
        <property type="entry name" value="UCP016817_carboligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp fold PylC-type" evidence="1">
        <location>
            <begin position="116"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02655"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95719.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="349" mass="40444" checksum="414DBE506E8E0ABD" modified="2013-06-26" version="1">MSQSLGDLGCYKALVLGVNTRAVANSLKSLGFYVYSVSYYAPEDLKADEKFFFVNPYKHGKLKDNYDPKKLLEKAKEIADSVDYIFITSGVFEYIKKAPKWENVVGNEPKKIVKLSNKYKVYKKLENLGYNIPTYKIIYNRHQLEKFREEFKKIVLKPISGCGGLAKNLEFPILAQEFIKSESFSINFIDNTFITFNKQIIINGQYAGNITPYKLSKTYVNELRDIIEVFELEGMNGLDFLINNNLLYIIDLNPRILGTFDTIEISSRKNLVKALLNKEYAKTVKPNRVYEKRIIFANRRLKVRIKNYRCLYDIPKNGAIIEKDEPIFTVISDSNIKNIIRESVKYEDT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTW3</accession>
    <name>N6UTW3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Molybdate ABC transporter periplasmic substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06003</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the bacterial solute-binding protein 1 family. WtpA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95789.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593059.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTW3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00558"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1169"/>
    <dbReference type="OrthoDB" id="7820at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030973">
        <property type="term" value="F:molybdate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901359">
        <property type="term" value="F:tungstate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015689">
        <property type="term" value="P:molybdate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd13540">
        <property type="entry name" value="PBP2_ModA_WtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.10:FF:000440">
        <property type="entry name" value="Uncharacterized solute-binding protein MA_0280"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022498">
        <property type="entry name" value="ABC_trnspt_W-bd_WtpA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050682">
        <property type="entry name" value="ModA/WtpA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003196">
        <property type="entry name" value="PRK04168.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03730">
        <property type="entry name" value="tungstate_WtpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30632">
        <property type="entry name" value="MOLYBDATE-BINDING PERIPLASMIC PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30632:SF16">
        <property type="entry name" value="MOLYBDATE_TUNGSTATE-BINDING PROTEIN WTPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13531">
        <property type="entry name" value="SBP_bac_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0732">Signal</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95789.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="330" mass="37724" checksum="0A5DBABBDF320A96" modified="2013-06-26" version="1">MKKLLLSIMVLSCLILAAGCVSSEKSKKEIIIFHAGSLSVPLEKYEKLFEKEYNVDVVREPAGSVKCVRKVTELNKKADILALADYSLIPKMLMPKYTDWYVMFARNELVLAYTNKSKYSNEINKDNWYEILNRDGVKFGFSNPNDDPCGYRAVMVIKLASMYYNKPIFKNLIENNSNIKAEGNNIIVPKEVKVNTNKIFMRSKETDLLAPLEVGAFDYLFIYKSVAKQHNLRYIELPKEINLGYYEFKDYYKKVSIQFLANNKTIYGKPIVYGLTILNNAPNKKDAILFVKFMLEHPEVLKECGQPPITPAIAVGNVPEEIKSLVVVKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTW8</accession>
    <name>N6UTW8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Glucose-1-phosphate cytidylyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05913</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000046">
        <property type="protein sequence ID" value="ENN95794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592965.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTW8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00989"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1153"/>
    <dbReference type="OrthoDB" id="15372at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047343">
        <property type="term" value="F:glucose-1-phosphate cytidylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02524">
        <property type="entry name" value="G1P_cytidylyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046981">
        <property type="entry name" value="G1P_cyt_trans"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013446">
        <property type="entry name" value="G1P_cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005835">
        <property type="entry name" value="NTP_transferase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02623">
        <property type="entry name" value="G1P_cyt_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47183:SF1">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE CYTIDYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47183">
        <property type="entry name" value="GLUCOSE-1-PHOSPHATE CYTIDYLYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00483">
        <property type="entry name" value="NTP_transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleotidyl transferase" evidence="1">
        <location>
            <begin position="2"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="257" mass="29759" checksum="A576B09383BCC7FE" modified="2013-06-26" version="1">MKVVILAGGFGTRLSEETHRIPKPMVEIGGKPILWHIMKIYSTYGYNDFIICLGYKGYIIKEYFANYFLHNSDVTIDLKTNEIEVHNNYSEPWRVTLVDTGLHTQTGGRIKRVAKYIGDETFMMTYGDGVGNINIRELVEFHKKHGKLATVTAVQPEGRFGVLKLNGNQVIKFAEKMDNKDSWINAGFFVLEPEVIDYIKGDDTIWEKEPLERLAEDGQLMAYFHRGFWKPMDKLKDKMELEKLWNSGNAPWKIWKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTY6</accession>
    <name>N6UTY6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">30S ribosomal protein S6 modification protein RimK</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_05743</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95814.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592804.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTY6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01256"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1120"/>
    <dbReference type="OrthoDB" id="33241at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="Rp_bS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053432">
        <property type="entry name" value="THMPT_Glu_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040720">
        <property type="entry name" value="MptN_Meth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF0">
        <property type="entry name" value="BETA-CITRYLGLUTAMATE SYNTHASE B-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="5">
        <location>
            <begin position="101"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95814.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="289" mass="33424" checksum="A0E8DE0E480192F3" modified="2013-06-26" version="1">MKIGIISIERDNVVNDIIESCKKLNVDYKLVNPENIAIGINMDFNIKYFKSSLLNIPIYFVRNLGYDNIFNFDILRYLENFSDIVNPPKGIEFAGNKFLTSMMLEKYNLPQPKTVVTSSISEAIYWIDCFEDVVIKPLIGCRGEGIIRIKKDMTITNKLEKLNKFKEKYKTFYIQEFIQPISNEYKDIRAFVIDDEVFAYYRIGGDNWKNNVSCGAKIEMCRINDELEDLAIKAKEALNLFYAGVDVIESKDGYKVLEVNATPSWEGLKKLNINISDILVKKMIRFSKC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU18</accession>
    <name>N6UU18_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Nicotinate-nucleotide pyrophosphorylase [carboxylating]</fullName>
            <ecNumber evidence="6">2.4.2.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Quinolinate phosphoribosyltransferase [decarboxylating]</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_05633</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Involved in the catabolism of quinolinic acid (QA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>nicotinate beta-D-ribonucleotide + CO2 + diphosphate = quinolinate + 5-phospho-alpha-D-ribose 1-diphosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:12733"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57502"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.19"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 6">Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from quinolinate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Hexamer formed by 3 homodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the NadC/ModD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.19" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95849.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592694.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU18"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00267"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1099"/>
    <dbReference type="OrthoDB" id="115072at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00331"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004514">
        <property type="term" value="F:nicotinate-nucleotide diphosphorylase (carboxylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034213">
        <property type="term" value="P:quinolinate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01572">
        <property type="entry name" value="QPRTase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000030">
        <property type="entry name" value="Nicotinate-nucleotide pyrophosphorylase, carboxylating"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.20">
        <property type="entry name" value="Quinolinate phosphoribosyl transferase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004393">
        <property type="entry name" value="NadC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027277">
        <property type="entry name" value="NadC/ModD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036068">
        <property type="entry name" value="Nicotinate_pribotase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037128">
        <property type="entry name" value="Quinolinate_PRibosylTase_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002638">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022412">
        <property type="entry name" value="Quinolinate_PRibosylTrfase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00078">
        <property type="entry name" value="nadC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32179:SF3">
        <property type="entry name" value="NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE [CARBOXYLATING]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01729">
        <property type="entry name" value="QRPTase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02749">
        <property type="entry name" value="QRPTase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006250">
        <property type="entry name" value="NadC_ModD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51690">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54675">
        <property type="entry name" value="Nicotinate/Quinolinate PRTase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3 6" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase N-terminal" evidence="9">
        <location>
            <begin position="22"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="Quinolinate phosphoribosyl transferase C-terminal" evidence="8">
        <location>
            <begin position="103"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="126"/>
            <end position="128"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="210"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="240"/>
            <end position="242"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="261"/>
            <end position="263"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRNR" id="PIRNR006250"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006250-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95849.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="280" mass="31450" checksum="8F9633B782474355" modified="2013-06-26" version="1">MLKDYALKLLKKSLEIDVGFGDITSSILPKVNVEAVIVAKEECLLCGINFIEEFFNSYGIKTKKLANDGDLVNGEILILNGDAETILTLERTALNFLSHLSGVATYTYRIIKIAKEVNKKVKIACTRKTLPLLSPLQKYAVYIAGGDTHRFRLDDCILIKDNHIDIMGIKEAIRKAKKNASFTKKIEVEVRNLEELKLALEEKPDIIMLDNFSTDMVGKALKIINEFKEKNKFKPIIEVSGGINEKNIKEYAKYDVDVISIGALTHSAKSIDMSLKIKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU43</accession>
    <name>N6UU43_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Signal peptide peptidase SppA, 36K type</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_05499</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase S49 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000041">
        <property type="protein sequence ID" value="ENN95874.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592563.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU43"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01408"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1074"/>
    <dbReference type="OrthoDB" id="31107at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008236">
        <property type="term" value="F:serine-type peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07023">
        <property type="entry name" value="S49_Sppa_N_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004635">
        <property type="entry name" value="Pept_S49_SppA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002142">
        <property type="entry name" value="Peptidase_S49"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047272">
        <property type="entry name" value="S49_SppA_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00706">
        <property type="entry name" value="SppA_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42987">
        <property type="entry name" value="PEPTIDASE S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42987:SF4">
        <property type="entry name" value="PROTEASE SOHB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01343">
        <property type="entry name" value="Peptidase_S49"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0720">Serine protease</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase S49" evidence="6">
        <location>
            <begin position="104"/>
            <end position="255"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008683"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022825"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01343"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95874.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="300" mass="34206" checksum="836F84998F4B15FE" modified="2013-06-26" version="1">MRKIYIFIIALIVILIILFGLSLIIIENFNFGKPKIAKVYLYNEIYFDYSKKEFPWEERRDARYYINLLDKLMKDNNTKGVLLVINSPGGEVVASEELAEKVAELSKKKPVVVYIETLACSGAYMVAAPANYIVSQRHSIVGSIGVRMDIIHYYNLMKKLGVNVTTIKAGKYKDIGSPFRPITKEEREYLEKIINETYNYFVEWVAKYRHIPLNKVYKIADGKIYTGEDAVKLGLVDQVGSEEDAMKKLKELANVTNVEIEEYGLEGGYSLFGLAYYLGRGIGDSLKEINLGEFYGYINS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU76</accession>
    <name>N6UU76_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 13">Delta-aminolevulinic acid dehydratase</fullName>
            <ecNumber evidence="3 13">4.2.1.24</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">J422_05349</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="8 13">
            <text>2 5-aminolevulinate = porphobilinogen + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:58126"/>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <dbReference type="EC" id="4.2.1.24"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Porphyrin-containing compound metabolism; protoporphyrin-IX biosynthesis; coproporphyrinogen-III from 5-aminolevulinate: step 1/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="13">Homooctamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 14">Belongs to the ALAD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.24" evidence="3 13"/>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95904.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592384.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU76"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01317"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1044"/>
    <dbReference type="OrthoDB" id="8493at2157"/>
    <dbReference type="UniPathway" id="UPA00251">
        <property type="reaction ID" value="UER00318"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004655">
        <property type="term" value="F:porphobilinogen synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006782">
        <property type="term" value="P:protoporphyrinogen IX biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00384">
        <property type="entry name" value="ALAD_PBGS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000019">
        <property type="entry name" value="Delta-aminolevulinic acid dehydratase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001731">
        <property type="entry name" value="ALAD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030656">
        <property type="entry name" value="ALAD_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006762">
        <property type="entry name" value="PRK09283.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11458:SF0">
        <property type="entry name" value="DELTA-AMINOLEVULINIC ACID DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00490">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001415">
        <property type="entry name" value="Porphbilin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00144">
        <property type="entry name" value="DALDHYDRTASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01004">
        <property type="entry name" value="ALAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00169">
        <property type="entry name" value="D_ALA_DEHYDRATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0350">Heme biosynthesis</keyword>
    <keyword evidence="6 13 15" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0627">Porphyrin biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="193"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="9">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="128"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="272"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>5-aminolevulinate</name>
            <dbReference type="ChEBI" id="CHEBI:356416"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008055"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012053"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00020771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR001415-5"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU000515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU004161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN95904.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="320" mass="36076" checksum="EFED76B4BC5E7542" modified="2013-06-26" version="1">MLIRPRRLRKNKKIRDLVRESKLSKDDLIMPIFVDENIKDKKEITSMPNQYRFSIEKAIEEAIEIADLKIPAVILFGIPKYKDPIGSSAFKKDGVIQRTIRGIKKELGDELLVIADCCLCEYTSHGHCGIVKDGKVLNDETLDILTRIALSYAEAGADIIAPSDMMDGRVRAIREALEREGFEDTLIMSYSAKYASSFYGPFRDAADSKPKFGDRKSYQMDIGNAKEAMKEIELDIKEGADLILIKPALAYLDIIKMASEKFYVPIGGYSVSGEYAMVEAAAKLGYINREEVIYEILLSIKRAGADFIITYWAKEMAERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUC7</accession>
    <name>N6UUC7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">8-amino-7-ketopelargonate synthase</fullName>
            <ecNumber evidence="10">2.3.1.47</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_04885</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the decarboxylative condensation of pimeloyl-[acyl-carrier protein] and L-alanine to produce 8-amino-7-oxononanoate (AON), [acyl-carrier protein], and carbon dioxide.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>6-carboxyhexanoyl-[ACP] + L-alanine + H(+) = (8S)-8-amino-7-oxononanoate + holo-[ACP] + CO2</text>
            <dbReference type="Rhea" id="RHEA:42288"/>
            <dbReference type="Rhea" id="RHEA-COMP:9685"/>
            <dbReference type="Rhea" id="RHEA-COMP:9955"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:64479"/>
            <dbReference type="ChEBI" id="CHEBI:78846"/>
            <dbReference type="ChEBI" id="CHEBI:149468"/>
            <dbReference type="EC" id="2.3.1.47"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9 10">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Cofactor biosynthesis; biotin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 10">Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.47" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95949.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592069.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUC7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00514"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.957"/>
    <dbReference type="OrthoDB" id="9071at2157"/>
    <dbReference type="UniPathway" id="UPA00078"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008710">
        <property type="term" value="F:8-amino-7-oxononanoate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06454">
        <property type="entry name" value="KBL_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001917">
        <property type="entry name" value="Aminotrans_II_pyridoxalP_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050087">
        <property type="entry name" value="AON_synthase_class-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004723">
        <property type="entry name" value="AONS_Archaea/Proteobacteria"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00858">
        <property type="entry name" value="bioF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693:SF77">
        <property type="entry name" value="8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13693">
        <property type="entry name" value="CLASS II AMINOTRANSFERASE/8-AMINO-7-OXONONANOATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00599">
        <property type="entry name" value="AA_TRANSFER_CLASS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="7 9 10" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="11">
        <location>
            <begin position="26"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="9">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004746"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010008"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047715"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR604723-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95949.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="366" mass="41265" checksum="3DB257F39D8FA7B8" modified="2013-06-26" version="1">MFREVLKKEIDKIKDNNLYRELRKVKGINFSSNDYLCLSKHPEVIEALKNGAKYGVGSTGSRLTSGNINHEKLEEKMAKFKGTESSLLYPTGYMANLSISAICKKGDLILSDELNHASIIDGCRLSKADRLIYKHCDTNHLLDLLEKNYKNYNNIFIITDSVFSMDGDIAPLNELKKIADDFGAVLILDDAHATGVLGNGKGALEYFNIKPDDNIIIIVTLSKAIGCLGGCVCGVEELKDYLINTSRSFIFTTALPPAIVEAAIKAIEIIEEGKVVKKLKKNINLANKIFKSYNFINRENETPIYPFIFRDKTVKIAEYLIKNNIFCIPIRYPTVPKGSERIRISINVEHMKEDFIILCEKIKELW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUF6</accession>
    <name>N6UUF6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_05010</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95974.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592120.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUF6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00487"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.982"/>
    <dbReference type="OrthoDB" id="43333at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04631">
        <property type="entry name" value="CBS_archAMPK_gamma-repeat2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="8"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="87"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="151"/>
            <end position="206"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="226"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95974.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="276" mass="31543" checksum="1286FB4C032CDF5D" modified="2013-06-26" version="1">MRALDVVKNKEIVKIYPTTTIREALKIMNENRFRRLPIVYPNNKLVGIITAMDIVDFIGGGSKYNLIREKFNRNFVAAINQPVREIMTKEVITLDENDDIDMAIETFLNKNVGGVPILDKEGHFLSLITERDVIKALINKIDENETIEDYITRKIIYATPGERLKDVARTMVRNGFRRLPVVSEDKLVGIITATDFIKLLGSNWTFKKMEMGNVREITNVRIEEIMIKDVITANNNDKLKDVAKIMVEKDIGALPVVEDSKLIGIITEKDVIKYFT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUL3</accession>
    <name>N6UUL3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Cobalt-precorrin-5B C(1)-methyltransferase</fullName>
            <ecNumber evidence="5">2.1.1.195</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Cobalt-precorrin-6A synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5 7" type="primary">cbiD</name>
        <name evidence="7" type="ORF">J422_04800</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the methylation of C-1 in cobalt-precorrin-5B to form cobalt-precorrin-6A.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>Co-precorrin-5B + S-adenosyl-L-methionine = Co-precorrin-6A + S-adenosyl-L-homocysteine</text>
            <dbReference type="Rhea" id="RHEA:26285"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:60063"/>
            <dbReference type="ChEBI" id="CHEBI:60064"/>
            <dbReference type="EC" id="2.1.1.195"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 6/10.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CbiD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.195" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96029.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592015.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUL3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00978"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.939"/>
    <dbReference type="OrthoDB" id="10423at2157"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00227"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043780">
        <property type="term" value="F:cobalt-precorrin-5B C1-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2110.10">
        <property type="entry name" value="CbiD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00787">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002748">
        <property type="entry name" value="CbiD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036074">
        <property type="entry name" value="CbiD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00312">
        <property type="entry name" value="cbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863">
        <property type="entry name" value="COBALT-PRECORRIN-5B C(1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35863:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-5B C(1)-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01888">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026782">
        <property type="entry name" value="CbiD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111342">
        <property type="entry name" value="CbiD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="6" id="KW-0472">Membrane</keyword>
    <keyword evidence="2 5" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5" id="KW-0808">Transferase</keyword>
    <keyword evidence="6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="30"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96029.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="356" mass="39803" checksum="13FD59C7F5E8F45D" modified="2013-06-26" version="1">MIYDFRKKLKYGYTTGSCAAAGAFAALYFLKYNKKLDYVLIENSSGDKLIIPIEKLEKDKNRAKAVVRKYAGEDIDITDGIEVEVEVFLRNDGEIEIKGGKGIGIVTKDGLQIKKGEYAINPKPKEMIIKNILRLLDKGEGATVIISIPKGEELAKKTLNPKLGIVGGLSILGTTGIVKPMSNEAYIESLKLQIDVALANNYKDLVFVPGNIGRVYAKRILNVEEDRIVEVSNFWKELLDYANNKVEKITIFGHAGKIIKLAAGIYNTHSKVADARNEILAAYSSLFINNKDDIKKILYANTTEEVINILKKYNIVNDVFNLIAKRVIERAEERWNIKFNCIIIDMKGNILGKVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUU9</accession>
    <name>N6UUU9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">PGF-pre-PGF domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_04158</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000027">
        <property type="protein sequence ID" value="ENN96124.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591639.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUU9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01343"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.814"/>
    <dbReference type="OrthoDB" id="103676at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026453">
        <property type="entry name" value="PGF_pre_PGF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04213">
        <property type="entry name" value="PGF_pre_PGF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="440"/>
            <end position="459"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="203"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="1">
        <location>
            <begin position="393"/>
            <end position="432"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="1">
        <location>
            <begin position="393"/>
            <end position="420"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96124.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="463" mass="51204" checksum="B41D8AAB0CED0FA6" modified="2013-06-26" version="1">MIKIKNKTIVSFFITAILASMLVLTQTAAVSVSLVPSTQECDVGDVVNIDATVTILSSKGEWANIKNITLKVEKDGKVDTVPIPLTLEPGKTVLSNKSLSNTNSEVIEVKLLNTTAVYGYGYGYSYVNDSSYGYLNWHYGYGYGYGYSGYGYNVLNSPSNAIIKYNIKWKPNEAGTYKFTLIVSLKSGEMFASSCEVTVKGIEETTTGGGGKRTTTGEETSEERVKSLLESKPEKYIVKEIKVEPGKKVEISIDKEISSLAGLEKLIIELNKEMNLQVIVAIVKSLPSKVTAPPSGTPYSIFEIVFVDKDTGELVEPKGEVEFKVPKSWLKEKGFGKDEVVLERWDDIEDKWEDLPTELVSETGDGYKYKAKLERFCILSVVAKAATTTEKEIKEENKTTTTKEIKEETENKTEIKEENKTTSVEKQPSTEKPAKGANNMLIIAVILAIIILVAAYLIFMKKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUZ6</accession>
    <name>N6UUZ6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Large ribosomal subunit protein uL6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rpl6p</name>
        <name evidence="5" type="synonym">rpl6</name>
        <name evidence="7" type="ORF">J422_03988</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">This protein binds to the 23S rRNA, and is important in its secondary structure. It is located near the subunit interface in the base of the L7/L12 stalk, and near the tRNA binding site of the peptidyltransferase center.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the universal ribosomal protein uL6 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96184.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591413.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUZ6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00427"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.782"/>
    <dbReference type="OrthoDB" id="7144at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.930.12:FF:000008">
        <property type="entry name" value="50S ribosomal protein L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.930.12">
        <property type="entry name" value="Ribosomal protein L6, alpha-beta domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01365_A">
        <property type="entry name" value="Ribosomal_uL6_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000702">
        <property type="entry name" value="Ribosomal_uL6-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036789">
        <property type="entry name" value="Ribosomal_uL6-like_a/b-dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020040">
        <property type="entry name" value="Ribosomal_uL6_a/b-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019907">
        <property type="entry name" value="Ribosomal_uL6_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002359">
        <property type="entry name" value="Ribosomal_uL6_CS2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004037">
        <property type="entry name" value="PRK05518.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03653">
        <property type="entry name" value="uL6_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655:SF16">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11655">
        <property type="entry name" value="60S/50S RIBOSOMAL PROTEIN L6/L9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00347">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002162">
        <property type="entry name" value="Ribosomal_L6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56053">
        <property type="entry name" value="Ribosomal protein L6"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00700">
        <property type="entry name" value="RIBOSOMAL_L6_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="3 5 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="2 5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="6">
        <location>
            <begin position="13"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL6 alpha-beta" evidence="6">
        <location>
            <begin position="99"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01365"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00347"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96184.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="184" mass="21078" checksum="83F46A7AF37A3304" modified="2013-06-26" version="1">MPVAAYLEERIKIPENVEVEINGNEVIVRCGNKELRRVFTTPNNNIFIKKEGNEIVIYCHYPRRKDKAMLGTIRAHINNMIKGVTEGFTYKLKIRYAHFPMKVYVKDNEVIIENFLGEKHPRRAKILDGVTVKIQGEDVIVTGIDKEKVGQTAANIEQATKIKDRDPRVFQDGIYIVEKAGKPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV65</accession>
    <name>N6UV65_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_03298</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96254.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590955.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV65"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01046"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.646"/>
    <dbReference type="OrthoDB" id="30736at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023867">
        <property type="entry name" value="Sulphatase_maturase_rSAM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43273">
        <property type="entry name" value="ANAEROBIC SULFATASE-MATURATING ENZYME HOMOLOG ASLB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43273:SF3">
        <property type="entry name" value="ANAEROBIC SULFATASE-MATURATING ENZYME HOMOLOG ASLB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01384">
        <property type="entry name" value="thioether_bond_formation_requi"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="1"/>
            <end position="213"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023601"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96254.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="286" mass="33308" checksum="8BC0326CBD11FF92" modified="2013-06-26" version="1">MKFLILKVTDRCNLNCKYCYSLNKNNEDMKFRIAKKAINYLLNFDDNIKIQFTGGEPLLNFKLIEKVVNYTEDKGNFRYAIQTNGTLLGNYVEEIKELGINVGVSIDGLEVNDELRPYKDGRCSTLDTIKGIYLLEKYDIPFGVTIVVTNKNLPYLEEFVGYLIAVGVKSFSFDLLKPKKREDLKLMPNIREFEKVLNNLSKYPVYIKNLYQRKDNYCFLNKGDLLFVNEIGDIYPCPTLEGYFYMGNIKNFNGELFKAKSKGCFAREYLYKKLLREEKLWAGLNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVF9</accession>
    <name>N6UVF9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">NAD-dependent epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02624</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NAD(P)-dependent epimerase/dehydratase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96339.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590557.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVF9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00077"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.514"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05256">
        <property type="entry name" value="UDP_AE_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001509">
        <property type="entry name" value="Epimerase_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01370">
        <property type="entry name" value="Epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD-dependent epimerase/dehydratase" evidence="2">
        <location>
            <begin position="2"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007637"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96339.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="304" mass="34578" checksum="9024E53D489E37DF" modified="2013-06-26" version="1">MILITGGAGFIGSHVVDLFVNNGYDVIILDNLKTGNLNNVNEKAKFIKGDIRDKINLRDIEVVIHQAAQINVRYSVEDPKYDADVNILGTINLLEAIRKYEIEKIIYASSGGAVYGEPKYLPVDENHPISPISPYGLSKYVGEEYIKLYNRLYGIEFVILRYGNVYGERQDPKGEAGVISIFIDKMLRGERPVIFGNGKQTRDFVYVKDVAKANLLAMKWKNEIVNIGSGKETSVNELYKIISEELKFKEKPIYDKPREGEVYRIYLNIDKAKSLGWEPETGLKEGIRKVINWMRGSYDKLNKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVH5</accession>
    <name>N6UVH5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DsrE family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02699</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96354.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590588.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVH5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00092"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.529"/>
    <dbReference type="OrthoDB" id="45650at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00291">
        <property type="entry name" value="SirA_YedF_YeeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1260.10">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.40">
        <property type="entry name" value="TusA-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027396">
        <property type="entry name" value="DsrEFH-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003787">
        <property type="entry name" value="Sulphur_relay_DsrE/F-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001455">
        <property type="entry name" value="TusA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036868">
        <property type="entry name" value="TusA-like_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279:SF18">
        <property type="entry name" value="SULFUR CARRIER PROTEIN MJ0990-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33279">
        <property type="entry name" value="SULFUR CARRIER PROTEIN YEDF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02635">
        <property type="entry name" value="DsrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01206">
        <property type="entry name" value="TusA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75169">
        <property type="entry name" value="DsrEFH-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64307">
        <property type="entry name" value="SirA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01148">
        <property type="entry name" value="UPF0033"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0033" evidence="1">
        <location>
            <begin position="72"/>
            <end position="96"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS01148"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96354.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="271" mass="29971" checksum="183EABCAEF844C5E" modified="2013-06-26" version="1">MIREISVKGIKIPELLVERVLNKMKEGMLIIEAEDWQIDKIKDVAKKYNYKVNVEGNKIIIHIGKIAANRTINVVGATCPGPIMMVSDVLEKMNVGEVLEIIAGKNALTDLTEGLKGAGHEVLSIEELEDGNYRILIKKGEKKVEGVTTITIDELFIINTTGTGNAEKAYATFLMADVAKKMNLKPTIFLMMDGASLALKGECDKVKHPDFPKLGDLVRKAIKEGVKVYVCELSAKFRGINEENLEEGFEIAGAPTFLNYLSKPNVRPVWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVI9</accession>
    <name>N6UVI9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter substrate-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_02759</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell envelope</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the bacterial solute-binding protein 5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96364.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVI9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00103"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.541"/>
    <dbReference type="OrthoDB" id="194307at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042597">
        <property type="term" value="C:periplasmic space"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:1904680">
        <property type="term" value="F:peptide transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00995">
        <property type="entry name" value="PBP2_NikA_DppA_OppA_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.105.10">
        <property type="entry name" value="Dipeptide-binding Protein, Domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030678">
        <property type="entry name" value="Peptide/Ni-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039424">
        <property type="entry name" value="SBP_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023765">
        <property type="entry name" value="SBP_5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000914">
        <property type="entry name" value="SBP_5_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290">
        <property type="entry name" value="PERIPLASMIC BINDING COMPONENT OF ABC TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30290:SF10">
        <property type="entry name" value="PERIPLASMIC OLIGOPEPTIDE-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00496">
        <property type="entry name" value="SBP_bac_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002741">
        <property type="entry name" value="MppA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01040">
        <property type="entry name" value="SBP_BACTERIAL_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0732">Signal</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Solute-binding protein family 5" evidence="5">
        <location>
            <begin position="81"/>
            <end position="427"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96364.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="523" mass="59994" checksum="9E4666ACF30E4750" modified="2013-06-26" version="1">MRKILVLFLLIGFSCLFLGCTEKSENVGKSVESIKEVKYKDTVIIGYGKDPRLKGGKWGVGFFPKSEVLEKLVEYNLKNDEILPNLAESWEIKDGGKEIIFHLKKGVKFSDGTELDANAVAFTMKRLIVKGHSLAPKDVKVIDKYTVAIYFDKPGFFNLAKMAEYHLSIMSPTSVKPIGDPNGTLIKPIGTGPLAVKNYVEDQYSTYTPNIYWYERKKIKPKFKEFVLKVIPDENTRIMALRSGDVDGICDYVHGGSAYTPRNQLPILEKEGFKVYKRNIPLTWVIAFNYKKPPFNDPEVRKAVSLAINRDDIVKIFGNQVRPAWHGMFPPEAPGMKEANITYEYNPEKAREILKKKGLLGAKVNLIVDKSQSDQILVGQLIQSQLNKAGFNVNLEILEPGAYRQKRNSGDYDMRIYYIGGTDRRFYLRLYWRFYPGGKWDAYESEKVCSLCKDILENPENFDKEKRKQKLIEFYKALYDEMGVVPLYHDIMTVVLRPNVEVNESDFFKYAEPQFYSIGVKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVQ2</accession>
    <name>N6UVQ2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Zn-ribbon RNA-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02559</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96419.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590471.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVQ2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00761"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.501"/>
    <dbReference type="OrthoDB" id="35104at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044720">
        <property type="entry name" value="HVO_2753-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011668">
        <property type="entry name" value="HVO_2753-like_ZBP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011481">
        <property type="entry name" value="PRK14890.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40733:SF1">
        <property type="entry name" value="SMALL ZINC FINGER PROTEIN HVO-2753-LIKE ZINC-BINDING POCKET DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40733">
        <property type="entry name" value="ZINC-RIBBON RNA-BINDING PROTEIN INVOLVED IN TRANSLATION-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07754">
        <property type="entry name" value="HVO_2753_ZBP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Small zinc finger protein HVO-2753-like zinc-binding pocket" evidence="1">
        <location>
            <begin position="6"/>
            <end position="51"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF07754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96419.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="55" mass="6240" checksum="8EBE8D02C6103F69" modified="2013-06-26" version="1">MGKYICISCNAEIAPREKATRFLCPNCGEVEIVRCERCRKLNNPYKCPKCGFEGP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6UW85</accession>
    <name>N6UW85_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10 11">Protein translocase subunit SecY</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Protein transport protein SEC61 subunit alpha homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">secY</name>
        <name evidence="14" type="ORF">J422_01530</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10 11">The central subunit of the protein translocation channel SecYEG. Consists of two halves formed by TMs 1-5 and 6-10. These two domains form a lateral gate at the front which open onto the bilayer between TMs 2 and 7, and are clamped together by SecE at the back. The channel is closed by both a pore ring composed of hydrophobic SecY resides and a short helix (helix 2A) on the extracellular side of the membrane which forms a plug. The plug probably moves laterally to allow the channel to open. The ring and the pore may move independently.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Component of the Sec protein translocase complex. Heterotrimer consisting of alpha (SecY), beta (SecG) and gamma (SecE) subunits. The heterotrimers can form oligomers, although 1 heterotrimer is thought to be able to translocate proteins. Interacts with the ribosome. May interact with SecDF, and other proteins may be involved.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
        <subcellularLocation>
            <location evidence="1 12">Membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10 13">Belongs to the SecY/SEC61-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96564.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UW85"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00434"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.301"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0065002">
        <property type="term" value="P:intracellular protein transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006605">
        <property type="term" value="P:protein targeting"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3370.10:FF:000013">
        <property type="entry name" value="Protein translocase subunit SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3370.10">
        <property type="entry name" value="SecY subunit domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01465">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026593">
        <property type="entry name" value="SecY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002208">
        <property type="entry name" value="SecY/SEC61-alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030659">
        <property type="entry name" value="SecY_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023201">
        <property type="entry name" value="SecY_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00967">
        <property type="entry name" value="3a0501s007"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006341">
        <property type="entry name" value="PRK08568.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10906">
        <property type="entry name" value="SECY/SEC61-ALPHA FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00344">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004557">
        <property type="entry name" value="SecY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00303">
        <property type="entry name" value="SECYTRNLCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103491">
        <property type="entry name" value="Preprotein translocase SecY subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00755">
        <property type="entry name" value="SECY_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00756">
        <property type="entry name" value="SECY_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="6 10 12" id="KW-0653">Protein transport</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 10 12" id="KW-0811">Translocation</keyword>
    <keyword evidence="5 10 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 10 12" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="33"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="71"/>
            <end position="95"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="142"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="213"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="255"/>
            <end position="276"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="318"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="377"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="401"/>
            <end position="419"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005751"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01465"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU000537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU003484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU004349"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN96564.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="441" mass="48291" checksum="FABA720F68493F84" modified="2013-06-26" version="1">MENIMKSLIPILEKIPDVELPLREPSFKEKLKWTGLVLVLYFIMGTIDVYTAGAQIPAIFEFWQTITASRIGTLITLGIGPIVTAGIIMQLLVGAKIINIDLSIPENRALFQSTQKFLAILMSFVEATLFVGTGAFGSLSPLIALLVILQIALGSIVLIYLDEIVSKYGIGSGIGLFIAAGVSQTIFVGAFGPEGYLWKFFSSLLQGAPNIEYILPILGTIIVFLMVVYSECLRVEIPLAHGRIRGAIAKYPIKFVYVSNMPVILTAALFANIQLWGLALQKVGLPILGHFQNGKAVDGIAYYLSTPYSILSVLSDPIHAIIYLIAMIICCIFFGIFWVETTGLDPKTMAKRISSLDMAIKGFRKSEKAIEQRLKRYIPAITVMSSAFVGFLAAIADFIGALGGGTGVLLTVSIVYRMYEQLLREKITELHPVISKLIKMR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWQ1</accession>
    <name>N6UWQ1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Aspartate dehydrogenase</fullName>
            <ecNumber evidence="6">1.4.1.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_00366</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the L-aspartate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.21" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589755.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWQ1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00707"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.70"/>
    <dbReference type="OrthoDB" id="15415at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033735">
        <property type="term" value="F:aspartate dehydrogenase [NAD(P)+] activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01265">
        <property type="entry name" value="NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005106">
        <property type="entry name" value="Asp/hSer_DH_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002811">
        <property type="entry name" value="Asp_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022487">
        <property type="entry name" value="Asp_DH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020626">
        <property type="entry name" value="Asp_DH_prok"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03855">
        <property type="entry name" value="NAD_NadX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009829">
        <property type="entry name" value="PRK13303.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009830">
        <property type="entry name" value="PRK13304.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31873:SF6">
        <property type="entry name" value="ASPARTATE DEHYDROGENASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31873">
        <property type="entry name" value="L-ASPARTATE DEHYDROGENASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01958">
        <property type="entry name" value="Asp_DH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03447">
        <property type="entry name" value="NAD_binding_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4 9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="2" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aspartate/homoserine dehydrogenase NAD-binding" evidence="8">
        <location>
            <begin position="3"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate dehydrogenase" evidence="7">
        <location>
            <begin position="116"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008331"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR03855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF03447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="214" mass="23495" checksum="538224798AE5C21F" modified="2013-06-26" version="1" fragment="single">KLAELVGAKVCNSLDELVSEDLDLIVEAASVEAVEEVAEKALKSRKDVLIMSVGAFADKNVFLKLKRLAKENNRNIYIPSGAIGGLDIVKSLKYGEIEELILKTTKHPKALGLDINEKKKVFEGDVFEAIKRFPQNINISVTLSLASKKVAKVIIIADPNVKENIHEIFIKSSIGECYIKINNVPLEENPKTSALAAYSAVRLINEIKEVVKIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWT5</accession>
    <name>N6UWT5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">DNA primase large subunit PriL</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">priL</name>
        <name evidence="9" type="ORF">J422_00105</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Regulatory subunit of DNA primase, an RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication. Stabilizes and modulates the activity of the small subunit, increasing the rate of DNA synthesis, and conferring RNA synthesis capability. The DNA polymerase activity may enable DNA primase to also catalyze primer extension after primer synthesis. May also play a role in DNA repair.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="7">Binds 1 [4Fe-4S] cluster.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterodimer of a small subunit (PriS) and a large subunit (PriL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eukaryotic-type primase large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96804.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589710.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWT5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01147"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.21"/>
    <dbReference type="OrthoDB" id="46081at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00701">
        <property type="entry name" value="DNA_primase_lrg_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058560">
        <property type="entry name" value="DNA_primase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023642">
        <property type="entry name" value="DNA_primase_lsu_PriL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04104">
        <property type="entry name" value="DNA_primase_lrg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 7" id="KW-0408">Iron</keyword>
    <keyword evidence="6 7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 7" id="KW-0639">Primosome</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA primase large subunit C-terminal" evidence="8">
        <location>
            <begin position="187"/>
            <end position="316"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="193"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="293"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="311"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="317"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00701"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF04104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96804.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="340" mass="40640" checksum="D578BE1D90F73D1F" modified="2013-06-26" version="1">MILEYLEKFKDRDFLNDLKKYKKDILKKLKFIIDKDKEGLEVLKKVYGEFYFLIDYLLIILLSHTPYYNFFIKSYSELMKDYVEISRKVRVWEFIKVAGKANNNDLFLERLDVNNGYVDISEVKDIYAKEMIRVELKELGEMARKIKLPNEEIIKELLDEISIYLKDKVKYSFGGVKVDKVVKDIPLEWHPPCIRKILEDILSGGSPSHYARRSFVVYWFAAKFNPNLRPLDKDGNLVNLSALDIAKEEEIEKFIDEMIEIFKNVDDFNEEKTRYYIMHNIGYKVGHQRFTHCEYCKNWKDDKGKGLKEYCNPDEICMKKFIIHPLDYLCYKMKSEKHDK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>N6V041</accession>
    <name>N6V041_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="13">Alanine--tRNA ligase</fullName>
            <ecNumber evidence="13">6.1.1.7</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13">Alanyl-tRNA synthetase</fullName>
            <shortName evidence="13">AlaRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13 16" type="primary">alaS</name>
        <name evidence="16" type="ORF">J422_06623</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editing domain.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>tRNA(Ala) + L-alanine + ATP = L-alanyl-tRNA(Ala) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:12540"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:9923"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57972"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78497"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.7"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="13">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 13">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="13">Consists of three domains; the N-terminal catalytic domain, the editing domain and the C-terminal C-Ala domain. The editing domain removes incorrectly charged amino acids, while the C-Ala domain, along with tRNA(Ala), serves as a bridge to cooperatively bring together the editing and aminoacylation centers thus stimulating deacylation of misacylated tRNAs.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13">Belongs to the class-II aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.7" evidence="13"/>
    <dbReference type="EMBL" id="APMM01000056">
        <property type="protein sequence ID" value="ENN95663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V041"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00364"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1289"/>
    <dbReference type="OrthoDB" id="7506at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004813">
        <property type="term" value="F:alanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006419">
        <property type="term" value="P:alanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00673">
        <property type="entry name" value="AlaRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.130:FF:000010">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.54.20:FF:000005">
        <property type="entry name" value="Alanine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.980.10:FF:000004">
        <property type="entry name" value="Alanine--tRNA ligase, cytoplasmic"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.310.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.54.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.550">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.980.10">
        <property type="entry name" value="Threonyl-trna Synthetase, Chain A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00036_A">
        <property type="entry name" value="Ala_tRNA_synth_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002318">
        <property type="entry name" value="Ala-tRNA-lgiase_IIc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018162">
        <property type="entry name" value="Ala-tRNA-ligase_IIc_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018165">
        <property type="entry name" value="Ala-tRNA-synth_IIc_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018164">
        <property type="entry name" value="Ala-tRNA-synth_IIc_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022429">
        <property type="entry name" value="Ala-tRNA_lgiase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050058">
        <property type="entry name" value="Ala-tRNA_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018163">
        <property type="entry name" value="Thr/Ala-tRNA-synth_IIc_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012947">
        <property type="entry name" value="tRNA_SAD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03683">
        <property type="entry name" value="A-tRNA_syn_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00344">
        <property type="entry name" value="alaS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777:SF9">
        <property type="entry name" value="ALANINE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11777">
        <property type="entry name" value="ALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01411">
        <property type="entry name" value="tRNA-synt_2c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07973">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00980">
        <property type="entry name" value="TRNASYNTHALA"/>
    </dbReference>
    <dbReference type="SMART" id="SM00863">
        <property type="entry name" value="tRNA_SAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101353">
        <property type="entry name" value="Putative anticodon-binding domain of alanyl-tRNA synthetase (AlaRS)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55186">
        <property type="entry name" value="ThrRS/AlaRS common domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50860">
        <property type="entry name" value="AA_TRNA_LIGASE_II_ALA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12 13" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="9 13" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="14" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 13" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 13 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 13" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11 13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 13" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 13" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="8 13" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Alanyl-transfer RNA synthetases family profile" evidence="15">
        <location>
            <begin position="50"/>
            <end position="731"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="14">
        <location>
            <begin position="754"/>
            <end position="784"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="581"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="585"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="688"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="692"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN95663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="866" mass="100221" checksum="852EA3C1D05480BD" modified="2013-06-26" version="1">MKVKLFEDLGFKRQKCEKCGKYFWSIEEKDICDDCDKYSFIGKKVTKKSFDYDEMVKEFIKFFEEHGHRHIKRAPVVARRWRDDILLTIASIAVFQPWVTKGLIKPKANPLVIAQPCIRLNDIDNVGRTGRHLTCFTMGGHHAFNTPENYIYWQDETVELCYKFFKRLGIDEKKIIFVESWWEGGGNAGPCFEVLTHGVELATLVFMQYEKIENDYKEIPLKIVDTGYGIERFVWISQGKETIYDAIFGNIVEKLKKEVGVEEIERGILKNITELSGIMDISDAKDLRELRKKVAEKVGMDVEELTRLLEPYENIYAIADHTRALAFMLGDGIVPSNVQEGYLVRMLIRKTLRHMDKLNLSLPITEIVRMQLNSLKSLYPELLEMEDYIMDVLQIEERRYRETLKRGEGIVRRLLKKKDISLDDLIELYDSHGLPVEIAKEILNKYGKDIDIPDNFYTLIAERHEHKKIEKKKEKLPEVDVQETELLFYKYPNDKEFEAEILKIVDNYVILDRTLFYPEGGGQKADKGKIIKGDKEYEVIDVKKENNIVYHKLKSVEGLKEGDKVKGVIDFERRLTLMRHHTATHIVNAACQRVLGKHVYQAGSDVDVDKARLDITHYKRISKEELKEIEKLANEIVLGNYKVESVFMDRNEAEEKFGFRIYQGGVVPGDVLRIVMIKNDELIDVQACGGTHCQNTGEVGMIKIIKTERIQDGVERLIYVSGLRALEYANTLEDILDKSSKILRCSYEDLPKSLEKIFEENKELNKKLEEMREKLAELKKYELLDKFEKIGDYEVLIECVDAEPKDLLSLADNLAIGNRIVILTNNKGFILCKRGEKVDVSMNELIKAIAKGGGKEHLAQEDVREN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6V062</accession>
    <name>N6V062_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Dihydroorotate oxidase B, electron transfer subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">pyrK</name>
        <name evidence="13" type="ORF">J422_06371</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the PyrD type B subunit to the ultimate electron acceptor NAD(+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>FAD</name>
            <dbReference type="ChEBI" id="CHEBI:57692"/>
        </cofactor>
        <text evidence="10">Binds 1 FAD per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="10">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="11">Binds 1 [2Fe-2S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD(+) route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Heterotetramer of 2 PyrK and 2 PyrD type B subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the PyrK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593520.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V062"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01299"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1240"/>
    <dbReference type="OrthoDB" id="35401at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00945"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050660">
        <property type="term" value="F:flavin adenine dinucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06220">
        <property type="entry name" value="DHOD_e_trans_like2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.80">
        <property type="entry name" value="Nucleotide-binding domain of ferredoxin-NADP reductase (FNR) module"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01211">
        <property type="entry name" value="DHODB_Fe_S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012165">
        <property type="entry name" value="Cyt_c3_hydrogenase_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019480">
        <property type="entry name" value="Dihydroorotate_DH_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023455">
        <property type="entry name" value="Dihydroorotate_DHASE_ETsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017927">
        <property type="entry name" value="FAD-bd_FR_type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039261">
        <property type="entry name" value="FNR_nucleotide-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050353">
        <property type="entry name" value="PyrK_electron_transfer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017938">
        <property type="entry name" value="Riboflavin_synthase-like_b-brl"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000796">
        <property type="entry name" value="PRK00054.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43513:SF3">
        <property type="entry name" value="DIHYDROOROTATE DEHYDROGENASE B (NAD(+)), ELECTRON TRANSFER SUBUNIT-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10418">
        <property type="entry name" value="DHODB_Fe-S_bind"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006816">
        <property type="entry name" value="Cyc3_hyd_g"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52343">
        <property type="entry name" value="Ferredoxin reductase-like, C-terminal NADP-linked domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63380">
        <property type="entry name" value="Riboflavin synthase domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51384">
        <property type="entry name" value="FAD_FR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10 11" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="7 10" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5 10" id="KW-0274">FAD</keyword>
    <keyword evidence="2 10" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="8 10 11" id="KW-0408">Iron</keyword>
    <keyword evidence="9 10 11" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 10 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6 10" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 10" id="KW-0813">Transport</keyword>
    <feature type="domain" description="FAD-binding FR-type" evidence="12">
        <location>
            <begin position="1"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="208"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="211"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 11">
        <location>
            <position position="220"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR006816-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN95693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="238" mass="26515" checksum="563C700E1DD8E1E2" modified="2013-06-26" version="1">MYEVCKIINIIDETPTVKTFVLDKSFKFKPGQFAMIWLPEVNEKPFSFASENSFTIAKVGPFTDKMHKLKIGDKIAVRGPYGSYFKQYGEKILAVAGGVGAVPIVTAVEAFNAEITTILGARNKDELLFIDRFKKHSKLLICTDDGSLGFKGYTTDLMEKVLKEESFDSIIACGPEVMLKKVVKIAYEHNIPVQVSMERYMKCGIGVCGHCCLDNGLCVCKDGPVFTGEQLRKIIIYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1E3</accession>
    <name>N6V1E3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">A-ATP synthase subunit H N-terminal archaeal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_04393</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96078.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591786.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1E3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00645"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.861"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.2950">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049501">
        <property type="entry name" value="A-ATP_synth_H_N_arc"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21585">
        <property type="entry name" value="A-ATP_synth_H_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="A-ATP synthase subunit H N-terminal archaeal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="43"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="7"/>
            <end position="86"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF21585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96078.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="102" mass="11594" checksum="22CE14D2AA1210D6" modified="2013-06-26" version="1">MNVMEAIKEVRLAEENAIKEIETAKKRAEEIKLEAIEEGKMIIKSAEEEAKNIVEEKIKKAEEEGNDIAKKIKDEAEQKVEELISIAKARIFSLKLSDVLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1I0</accession>
    <name>N6V1I0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Rubrerythrin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_04053</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Fe(3+)</name>
            <dbReference type="ChEBI" id="CHEBI:29034"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000026">
        <property type="protein sequence ID" value="ENN96128.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591527.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1I0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00724"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.794"/>
    <dbReference type="OrthoDB" id="45654at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00729">
        <property type="entry name" value="rubredoxin_SM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd01041">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003251">
        <property type="entry name" value="Rr_diiron-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052364">
        <property type="entry name" value="Rubrerythrin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048574">
        <property type="entry name" value="RUBY_RBDX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045767">
        <property type="entry name" value="RuberyRbr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43865">
        <property type="entry name" value="RUBRERYTHRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43865:SF1">
        <property type="entry name" value="RUBRERYTHRIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02915">
        <property type="entry name" value="Rubrerythrin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21349">
        <property type="entry name" value="RUBY_RBDX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="6">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001965"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96128.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="189" mass="21894" checksum="6470E40F2CECB577" modified="2013-06-26" version="1">MKTLENLVKAFIGECVARNRYTFYAKIAKKEGYVFVSKVFLETAENEKEHAENLYKAIQELRDGDVFSVNVEIPIALGNTRDVLKIAIEGESYEHNKMYPEFAKVAEEEGFDDISKMLNAISIAEKHHEDRYRKILSVIENGTMFKRDKEVEWVCLECGYVHHGYEPPEKCPSCKHDRGYYVSRDLLSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1T5</accession>
    <name>N6V1T5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03283</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96253.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590954.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1T5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01044"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.643"/>
    <dbReference type="OrthoDB" id="227825at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007381">
        <property type="entry name" value="CheF1/F2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42201">
        <property type="entry name" value="TAXIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42201:SF1">
        <property type="entry name" value="TAXIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04283">
        <property type="entry name" value="CheF-arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96253.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="343" mass="39396" checksum="98F5A3A10145D1C5" modified="2013-06-26" version="1">MAKKELAKFQGKGILLNQYTIKSPVLKWEKLEITLYEDSLLFEFKDKKIEVELQYVEDVGAGLPRRAIEVAKTSLEDITYHSSITYKLPESDKTMIGFAPETSIYGRKPIENFLRKLFYALLNKKEVKVQYGAIKGGTVDPNVKWEDGYLVFAKVKSLLSPGEVLAVAVLEDGKPKVYNLFTNLESIRIKKKMVGEEEKDVLEIKQIKGNENITSYLYVEPRERLFILRYIENLTKYKNVVKDLLPKTEDELTSEFAAEEFSGERIKSELEKLTPQEQEILMALYTGISSLDLPNMLNMDVDEVERILDDLIEKGLVTLVRVRKEVELSEKGRAITNYIVSNF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1V8</accession>
    <name>N6V1V8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Acetyl-CoA decarbonylase/synthase complex subunit alpha</fullName>
            <shortName evidence="8">ACDS complex subunit alpha</shortName>
            <ecNumber evidence="8">1.2.7.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">ACDS complex carbon monoxide dehydrogenase subunit alpha</fullName>
            <shortName evidence="8">ACDS CODH subunit alpha</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">cdhA</name>
        <name evidence="10" type="ORF">J422_03206</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the ACDS complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). The alpha-epsilon subcomponent functions as a carbon monoxide dehydrogenase.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>CO + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O = 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:21040"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="EC" id="1.2.7.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="8">Binds 7 [4Fe-4S] clusters per heterotetramer.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </cofactor>
        <text evidence="8">Binds 2 [Ni-4Fe-4S] clusters per heterotetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterotetramer of two alpha and two epsilon subunits. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta subunits with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="domain">
        <text evidence="8">Cluster B is an all-cysteinyl-liganded 4Fe-4S cluster; cluster C is a mixed Ni-Fe-S cluster which is the active site of CO oxidation. Cluster D is also an all-cysteinyl-liganded 4Fe-4S cluster that bridges the two subunits of the CODH dimer. Contains two additional 4Fe-4S clusters, dubbed E and F, that probably transport electrons from ferredoxin to the B cluster.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Ni-containing carbon monoxide dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.4" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000019">
        <property type="protein sequence ID" value="ENN96278.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1V8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00318"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.629"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043885">
        <property type="term" value="F:anaerobic carbon-monoxide dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050418">
        <property type="term" value="F:hydroxylamine reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004601">
        <property type="term" value="F:peroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006084">
        <property type="term" value="P:acetyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042542">
        <property type="term" value="P:response to hydrogen peroxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2030">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.190">
        <property type="entry name" value="Carbon monoxide dehydrogenase alpha subunit. Chain M, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01137">
        <property type="entry name" value="CdhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004460">
        <property type="entry name" value="CdhA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004137">
        <property type="entry name" value="HCP/CODH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016099">
        <property type="entry name" value="Prismane-like_a/b-sand"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011254">
        <property type="entry name" value="Prismane-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00314">
        <property type="entry name" value="cdhA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109:SF6">
        <property type="entry name" value="ACETYL-COA DECARBONYLASE_SYNTHASE COMPLEX SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30109">
        <property type="entry name" value="HYDROXYLAMINE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03063">
        <property type="entry name" value="Prismane"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56821">
        <property type="entry name" value="Prismane protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0533">Nickel</keyword>
    <keyword evidence="5 8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="385"/>
            <end position="414"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="66"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>1</label>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="68"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="73"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="106"/>
        </location>
        <ligand>
            <name>CO</name>
            <dbReference type="ChEBI" id="CHEBI:17245"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="239"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="267"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="395"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="398"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="401"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
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        <location>
            <position position="405"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
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        <location>
            <position position="433"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
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        <location>
            <position position="436"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="439"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>4</label>
        </ligand>
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        <location>
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        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <label>3</label>
        </ligand>
    </feature>
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        <location>
            <position position="501"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
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    <feature type="binding site" evidence="8">
        <location>
            <position position="530"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
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        <location>
            <position position="565"/>
        </location>
        <ligand>
            <name>[Ni-4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:47739"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96278.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="761" mass="84723" checksum="3B0791D7B988ADD6" modified="2013-06-26" version="1">MGMVGDLVIPSVKMKKANISFSINIGEKEEPKGKTPKPGLLTLRSWDFKLLERYKPFYMPICDLCCLCTFGKCDLSKGKRGACGIDIRAQQARIVLLACCIGTACHTAHARHLVDHLIEELGEDYEINLGNEIDVKMPITRVVMGYEPKTLGDLRDVISYCEEQLTHGLSATHTGQEGSYLDFESKALHMGMIDDLAREVGDIAQIISYNMPKGEETDLADLGFDAIDKKKPVILCIGHNVIPGSYIIDYLEEKDLEDEIEVCGICCTAIDINRVSNKTKIVGPLSKQIMFVRAGVADVVIVDEQCINTNILTETLNTGAIFIATNDKACHGLEDISHLSPEEMVAYLLKHRAALITDMEKLAEVAVELAKIVAKNRKKDILPTVKEIVELAKQCTECGWCNRNCPCAFEIKEAMILAKKGDLKGLAEIYKKCYSCGRCEAICERNLPIVSMITKAGIYHFTNQRYKIRIGRGPVTDVEIRKVGAPIVFGDIPGVVGFAGCSALPNGEEEVALMAKELLERKYIVVATGCSAMAIAMWKDKDGKTLYEKYPSEFKAGGLLNLGPCLANAHISGAAIKIANIFAKKPLEGNYEEIADYILNKVGAVGVAWGAMSQKAAAIATGFNRWGIPVIIGPHGAKYRRLYLSDGNRFKVRDKNTKEILEIDPCPEHLAITAENYKEALCLIPKLCMRPNDTPKGRANKIYHYVTMYEKYFNRMPPDLEKFVRTEQDIPFMLKDKIKEYLDEKNWKPLEKYPKEPTLLW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6V221</accession>
    <name>N6V221_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_02594</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="6">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96333.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590546.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V221"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00071"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.508"/>
    <dbReference type="OrthoDB" id="5682at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027596">
        <property type="entry name" value="AmmeMemoSam_rS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034457">
        <property type="entry name" value="Organic_radical-activating"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016431">
        <property type="entry name" value="Pyrv-formate_lyase-activ_prd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04337">
        <property type="entry name" value="AmmeMemoSam_rS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352:SF5">
        <property type="entry name" value="PYRUVATE FORMATE-LYASE 1-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30352">
        <property type="entry name" value="PYRUVATE FORMATE-LYASE-ACTIVATING ENZYME"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004869">
        <property type="entry name" value="PflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01101">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="4 6" id="KW-0408">Iron</keyword>
    <keyword evidence="5 6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="66"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR004869-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96333.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="330" mass="37671" checksum="493C4BB3C9E4A802" modified="2013-06-26" version="1">MKEAMFYKNINGDVICLLCPRHCVIKEGKRGFCYNRENINGKLYAVGYGKVCSLAIDPIEKKPLYHFYPGSKVLSLAIGGCNFRCLHCQNWTISQVPPDKTVYREMSPEDVIEIALEYNLPGLSYTYTEPTVFYEFMYDCSILAKKYGLFNTMVTNGYIEKEPLKALPIDAMNIDIKGNERFYKEVCKAELEPVLKTCVLAKKLNIWVEITNLIIPGYNDSEDDILFIIEFVRDKLGRETPLHFSRFHPDYKLTNVPPTPIETLIKARELALEEGLKYVYIGNVPGLGENTYCPNCGALLIERSLFSVRFVNLEGDRCSVCGEKIDIITR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6V243</accession>
    <name>N6V243_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Sugar phosphate permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02694</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96353.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590586.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V243"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00091"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.528"/>
    <dbReference type="OrthoDB" id="65971at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035350">
        <property type="entry name" value="DUF5400"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17379">
        <property type="entry name" value="DUF5400"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="91"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96353.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12237" checksum="49317C153771D45D" modified="2013-06-26" version="1">MNTVLMIMSLSVIFGAMLSGFATFRMTGMRFMPHFVSLILAFILTLASLFINNILLFYLAIAFQIITPITICGTICNILKTQFQNTGIYSSHLALMGMLFILAIGNLLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6V261</accession>
    <name>N6V261_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SagB-type dehydrogenase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02779</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96368.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590616.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V261"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00107"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.545"/>
    <dbReference type="OrthoDB" id="10206at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02142">
        <property type="entry name" value="McbC_SagB-like_oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.109.10">
        <property type="entry name" value="NADH Oxidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052544">
        <property type="entry name" value="Bacteriocin_Proc_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029479">
        <property type="entry name" value="Nitroreductase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000415">
        <property type="entry name" value="Nitroreductase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020051">
        <property type="entry name" value="SagB-type_dehydrogenase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03605">
        <property type="entry name" value="antibiot_sagB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43745">
        <property type="entry name" value="NITROREDUCTASE MJ1384-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43745:SF2">
        <property type="entry name" value="NITROREDUCTASE MJ1384-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00881">
        <property type="entry name" value="Nitroreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55469">
        <property type="entry name" value="FMN-dependent nitroreductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Nitroreductase" evidence="1">
        <location>
            <begin position="19"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96368.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="196" mass="22194" checksum="4D4ED057984369B0" modified="2013-06-26" version="1">MEILLPEIKNIKLNQLLIKRRSIRSFRDDPLTLEDLSNLLFAAYGITNNFGFRTVPSAGATFPLEIYVNIKNVIDVDEGVYKYIPERHSIVLHLSRDVSYDLAIASLNQMFIAEAPVVIIITADFYRTVKVYGNRGYRYVYMEAGSSYQNVYLMATALNLGTVAVGAFYDDKVKEILMIDEDPLILLPVGKPFKTV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2B3</accession>
    <name>N6V2B3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02494</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96408.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590449.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2B3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00774"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.488"/>
    <dbReference type="OrthoDB" id="63593at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96408.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="158" mass="18638" checksum="7959924483BDE072" modified="2013-06-26" version="1">MDVYEVLYQACLEYEVVLDGKRVPLWKVKKEDLEKVDFRLPWNSLRELAVHLYELKSKQQKSKELIRVNLVEILIGIAFLKVEDEFGSICNVEDLCLTYLSELITARINCIAKYYYLIKKPNNTDLFDEIILKFPQNKNIKAGNLNDLKELIFKLKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2B9</accession>
    <name>N6V2B9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Small ribosomal subunit protein eS17</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rps17E</name>
        <name evidence="4" type="synonym">rps17e</name>
        <name evidence="5" type="ORF">J422_02519</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein eS17 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96413.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590459.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2B9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00768"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.493"/>
    <dbReference type="OrthoDB" id="52479at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.20">
        <property type="entry name" value="Ribosomal protein S17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00511">
        <property type="entry name" value="Ribosomal_eS17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001210">
        <property type="entry name" value="Ribosomal_eS17"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036401">
        <property type="entry name" value="Ribosomal_eS17_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002242">
        <property type="entry name" value="PRK01151.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10732:SF0">
        <property type="entry name" value="40S RIBOSOMAL PROTEIN S17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00833">
        <property type="entry name" value="Ribosomal_S17e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116820">
        <property type="entry name" value="Rps17e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010444"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00511"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96413.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="63" mass="7587" checksum="8225A80D58F4DEE8" modified="2013-06-26" version="1">MGRIRQALIKRTAMELIKRFKDKFTTDFETNKKVLEEVAYVSTKRLRNRLAGYITHKMRQLSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2Y1</accession>
    <name>N6V2Y1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">NADH ubiquinone oxidoreductase 20 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_01460</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the complex I 20 kDa subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000010">
        <property type="protein sequence ID" value="ENN96588.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2Y1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01026"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.286"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12280">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052375">
        <property type="entry name" value="Complex_I_20kDa-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006137">
        <property type="entry name" value="NADH_UbQ_OxRdtase-like_20kDa"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42989:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42989">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01058">
        <property type="entry name" value="Oxidored_q6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56770">
        <property type="entry name" value="HydA/Nqo6-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NADH:ubiquinone oxidoreductase-like 20kDa subunit" evidence="7">
        <location>
            <begin position="20"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009173"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96588.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="131" mass="14176" checksum="AC3DE322D4BC1A07" modified="2013-06-26" version="1">MKTLARKKCIHVFLAYTGGCNGCDIEVVNAVLSPYYDAEQYNVFLTFNPREADILIVSGCVTLANLKPLKEIYKKIPEPKAVVAVGSCALMGGVYKNIYGDLGPSEFVVAPVDKVIPVDVKVPGCAPRLKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2Y4</accession>
    <name>N6V2Y4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Monovalent cation/H+ antiporter subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_01485</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000010">
        <property type="protein sequence ID" value="ENN96593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589958.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2Y4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01031"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.292"/>
    <dbReference type="OrthoDB" id="19265at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050622">
        <property type="entry name" value="CPA3_antiporter_subunitB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007182">
        <property type="entry name" value="MnhB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009229">
        <property type="entry name" value="PRK12579.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33932">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33932:SF4">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04039">
        <property type="entry name" value="MnhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="112"/>
            <end position="134"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="140"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="178"/>
            <end position="197"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="217"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="domain" description="Na+/H+ antiporter MnhB subunit-related protein" evidence="7">
        <location>
            <begin position="109"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF04039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="259" mass="28690" checksum="506ED6BC36E600A5" modified="2013-06-26" version="1">MSREIIVSISFLIFGVSLLLALWNIHVSPGVNLIYLKNYIIPNYVCAVVFDWRAYDTLGEALVLVVAVMISWVIFGKSLYDNSFLKELFKIPESDDYITLQKWGEFTNIIEFLAFPMSILMVGLGIITILGGHITPGGGFQGGALIAGAFILSVVAFGSKTPLWFDHKYLEKLEALGALGYLLIGLLGMVFGGYYLFNFYSVNGFIIFPSPKEIVTAGIIPYLNIFVGLKVLAGLSTASFLLSCEKVIIERIEKSEEKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2Y8</accession>
    <name>N6V2Y8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">Prefoldin subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GimC subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">pfdA</name>
        <name evidence="8" type="ORF">J422_01318</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 5">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 5">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the prefoldin alpha subunit family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the prefoldin subunit alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96598.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589927.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2Y8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00026"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.258"/>
    <dbReference type="OrthoDB" id="10045at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006457">
        <property type="term" value="P:protein folding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23160">
        <property type="entry name" value="Prefoldin_alpha_GimC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00308">
        <property type="entry name" value="PfdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011599">
        <property type="entry name" value="PFD_alpha_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004127">
        <property type="entry name" value="Prefoldin_subunit_alpha"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00293">
        <property type="entry name" value="prefoldin subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02996">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0143">Chaperone</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="4"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="84"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00025077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00308"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR00293"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96598.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="136" mass="15842" checksum="FA6B33E53489BA7F" modified="2013-06-26" version="1">MKNLEQKAMAYEMYSQQLQAIQNEIASIRALKNEINKTIETIENIKLDEETLIPVGPGVFLKAKIIEDKALLGIKSDLFIEKPFNETIEKLKNKLDELDKVEKEGIKRIEELSKIILELRKELEEAMKKEKEKKEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6V307</accession>
    <name>N6V307_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">AAA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01238</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96623.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589913.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V307"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00041"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.242"/>
    <dbReference type="OrthoDB" id="31168at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF10">
        <property type="entry name" value="ATPASE INVOLVED IN CHROMOSOME PARTITIONING, PARA_MIND FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="3"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96623.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="271" mass="30685" checksum="D287E35C936861DB" modified="2013-06-26" version="1">MKVGFYNIQGGTGKTTVAANFAYIVSQSTKTIFIDCDIYGGTSSIIFNLEDKEHNLASYLAGESVLEDIIYTYDDLSVIPSDMSSKVFAYKIDVSRLDHLLDTIEDEYDIIVYDFPPNITEDNPLLTYVGESELINKVIIVGEDSLPSIVNSLKTMELVKDLNIPTIGIIVNKYRGIVDLEDIIDDVIGVLPYDQKVERQWVESLPIAKLKTKFSKELQLLSKDIAKIYLEKDLASLRALKLAKTIYGLHEDEEEKEEEFSFNLNEKFKLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6V339</accession>
    <name>N6V339_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DegT/DnrJ/EryC1/StrS aminotransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01186</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96663.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589903.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V339"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.232"/>
    <dbReference type="OrthoDB" id="82426at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96663.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="295" mass="33657" checksum="921EB313046F18F3" modified="2013-06-26" version="1">MILRYRRVRLANKEGDKREVIRIINHLLNNEYDIIFLPSGSSGVFLSMFLADKFYIPDMGGWEGFLKYPKVLNKSFEILKTNFGIIDLDILSKIKGGALLVTSLAGYLAEQPLKEIRKICNENEILFIEDISGKIGGDCGYGDVIICSTGSPKILNCGYGGFLGIAKNSNLDLEKIKEIAKTYKTINYFGLLKEELIDAEKVYKTYIKANYKIKKEIDCYFKEHEGLSIFIECDNPKEKAKEINSKIMLDNFKSLTTICPNYNRLLKKGIVFETKKIDYKELEEDVINYIITTLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6V345</accession>
    <name>N6V345_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Precorrin-3B C17-methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_01006</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000006">
        <property type="protein sequence ID" value="ENN96673.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589869.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V345"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01017"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.196"/>
    <dbReference type="OrthoDB" id="35891at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11646">
        <property type="entry name" value="Precorrin_3B_C17_MT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1010.10">
        <property type="entry name" value="Cobalt-precorrin-4 Transmethylase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.950.10">
        <property type="entry name" value="Methyltransferase, Cobalt-precorrin-4 Transmethylase, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000878">
        <property type="entry name" value="4pyrrol_Mease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035996">
        <property type="entry name" value="4pyrrol_Methylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014777">
        <property type="entry name" value="4pyrrole_Mease_sub1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014776">
        <property type="entry name" value="4pyrrole_Mease_sub2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006363">
        <property type="entry name" value="Cbl_synth_CobJ/CibH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051810">
        <property type="entry name" value="Precorrin_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01466">
        <property type="entry name" value="cobJ_cbiH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47036:SF1">
        <property type="entry name" value="COBALT-FACTOR III C(17)-METHYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00590">
        <property type="entry name" value="TP_methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53790">
        <property type="entry name" value="Tetrapyrrole methylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="3 7" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="4 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Tetrapyrrole methylase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96673.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="251" mass="28822" checksum="C63ADA302DEC202A" modified="2013-06-26" version="1">MLYIVGLGSGERYLTKEAEEILNNVDLIVCYKGYKKYIEKFNKPTYTSGMTKEIDRVEKALSEAKNKDVALVSTGDPNVYGLASLAYELNYIKNYNVKIKVIPGITACLLAASILGSPLHDFVVISFSDLLTPLDKILKRFKLALEGDYVICIYNPLSKRRKEPFLKAVEIMREFNKNYIIGIVKNAGRENEEKRITDFKDLYKNLNEYLEYIDMNTIIIIGNSETYVIDNKMITPRGYLNKYKVIPCYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6V362</accession>
    <name>N6V362_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0179 protein J422_00941</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00941</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0179 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96693.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589858.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V362"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00586"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.185"/>
    <dbReference type="OrthoDB" id="24613at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00498">
        <property type="entry name" value="UPF0179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005369">
        <property type="entry name" value="UPF0179"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40699">
        <property type="entry name" value="UPF0179 PROTEIN MJ1627"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40699:SF1">
        <property type="entry name" value="UPF0179 PROTEIN MJ1627"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03684">
        <property type="entry name" value="UPF0179"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006595">
        <property type="entry name" value="UCP006595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00498"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96693.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="147" mass="16684" checksum="E5008EA409A241EF" modified="2013-06-26" version="1">MDKKITLIGSKLANIGEEFVYLGEVEECKTCKFRKLCHNNLEVGRKYKIVSVRSAEYSCPIHEGGVKVVEVELADIILNIDAKKALEGVVLNYEPINCGNFNCKNYKFCHPEGIYEGDKFRIKNVLNEKINCENGKTLKKVIVELIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6V390</accession>
    <name>N6V390_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">4Fe-4S ferredoxin iron-sulfur binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_00756</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96723.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589823.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V390"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00885"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.147"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10549">
        <property type="entry name" value="MtMvhB_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050157">
        <property type="entry name" value="PSI_iron-sulfur_center"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045874">
        <property type="entry name" value="Vhu_polyferre"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24960">
        <property type="entry name" value="PHOTOSYSTEM I IRON-SULFUR CENTER-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR24960:SF85">
        <property type="entry name" value="POLYFERREDOXIN PROTEIN VHUB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12800">
        <property type="entry name" value="Fer4_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="10"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="23"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="61"/>
            <end position="90"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="93"/>
            <end position="118"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="130"/>
            <end position="159"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="160"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="198"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="232"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="294"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="336"/>
            <end position="365"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96723.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="394" mass="43281" checksum="CC2B38E7624DAA91" modified="2013-06-26" version="1">MPIKIQKDACLVCYACQAECPTKAIDIDSFKVCTLCLKCAEVCPTGALTVEETEINGKKLKRISYNPSKCKKCGACAEACSVGIKKVDDEFPYSKGHCVLCLKCIETCPIEIISLPGVIDKPERKIPVVKEPIYVTDDCVGCSLCVPECPVNAITIEDGKAVIDKNKCIYCSICAQTCPWNAIIVAGKIPKKRRKEIKSFTVDKEKCIYCLKCVEVCPGNKDKIKLIKPNPEEFVVEPPKACPACQLCVNICPVDALHLDVKFSSPHPITDEGLVIIEEDYDILKKCASVCPTDAIVVDPEKKEVRMCIVCGACTVACPTGALKLGKIEHNGKEYNRIEYSPYLCNKCGKCVEVCPMKTLKLTKSRIPLKGYCVMCLLCLSVAEAEKKKVLMLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6V394</accession>
    <name>N6V394_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Peptidase U62 modulator of DNA gyrase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_00791</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase U62 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96728.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589828.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V394"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00878"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.155"/>
    <dbReference type="OrthoDB" id="98233at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008237">
        <property type="term" value="F:metallopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2290.10">
        <property type="entry name" value="PmbA/TldD superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045569">
        <property type="entry name" value="Metalloprtase-TldD/E_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045570">
        <property type="entry name" value="Metalloprtase-TldD/E_cen_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002510">
        <property type="entry name" value="Metalloprtase-TldD/E_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051463">
        <property type="entry name" value="Peptidase_U62_metallo"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025502">
        <property type="entry name" value="TldD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035068">
        <property type="entry name" value="TldD/PmbA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036059">
        <property type="entry name" value="TldD/PmbA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624:SF0">
        <property type="entry name" value="METALLOPROTEASE SLR0863"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30624">
        <property type="entry name" value="UNCHARACTERIZED PROTEIN TLDD AND PMBA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01523">
        <property type="entry name" value="PmbA_TldD_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19290">
        <property type="entry name" value="PmbA_TldD_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19289">
        <property type="entry name" value="PmbA_TldD_3rd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004919">
        <property type="entry name" value="TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111283">
        <property type="entry name" value="Putative modulator of DNA gyrase, PmbA/TldD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloprotease TldD/E N-terminal" evidence="5">
        <location>
            <begin position="27"/>
            <end position="89"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E central" evidence="7">
        <location>
            <begin position="120"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="Metalloprotease TldD/E C-terminal" evidence="6">
        <location>
            <begin position="231"/>
            <end position="435"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF01523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF19289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF19290"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96728.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="459" mass="50823" checksum="EDBD7D8EA16D6D57" modified="2013-06-26" version="1">MFIQRCGKGEILDIDKIIKLLDIGDYADVRINSGYSNSIVLKDGVFEEISFGMGKGIAVRVLYKNGWGFCKSKNIEDLEELIKKAYKMAKLANDNTKKEVILKDYKAINDKYKLIGKIAPYDVDIEEKKRIAIESYKAMRDEKIKSISVSYSDICGKKIFINSEGSFIEGEVGRAIMYMSCVAKENGRLEYASERIGGFGFENIKDNYLNKSKEAKDRALRMLKAKPCPKGEFKVILDHELAGVFIHEAVGHAAEADLVLQNDSVFKGKLNQKVGSEIVNVIDDPTLENGFGSYKYDDEGVEGKRTVIIENGILKSYLHSRETAGRLDMELTGNGRAEGLNRPIVRMSNTYIEPGDWSLDELIEDTKEGLFLKGSRGGQVDTGKGLFQFSAVEAYKIENGELKEVIKDAGLSGEILDILFKIDAITKDLKLSVGYCGKNGQSVPVGDGGGCVRTKTVIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3D4</accession>
    <name>N6V3D4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Proliferating cell nuclear antigen</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_00386</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB/NOP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96773.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589759.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3D4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00703"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.74"/>
    <dbReference type="OrthoDB" id="14725at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016428">
        <property type="term" value="F:tRNA (cytidine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1170">
        <property type="entry name" value="Sun protein, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049560">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001678">
        <property type="entry name" value="MeTrfase_RsmB-F_NOP2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011023">
        <property type="entry name" value="Nop2p"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023267">
        <property type="entry name" value="RCMT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018314">
        <property type="entry name" value="RsmB/NOL1/NOP2-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00446">
        <property type="entry name" value="nop2p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807">
        <property type="entry name" value="NOP2 YEAST -RELATED NOL1/NOP2/FMU SUN DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22807:SF74">
        <property type="entry name" value="TRNA (CYTOSINE(48)-C(5))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01189">
        <property type="entry name" value="Methyltr_RsmB-F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR02008">
        <property type="entry name" value="RCMTFAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01153">
        <property type="entry name" value="NOL1_NOP2_SUN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51686">
        <property type="entry name" value="SAM_MT_RSMB_NOP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="4 6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="SAM-dependent MTase RsmB/NOP-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="262"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="6">
        <location>
            <position position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="80"/>
            <end position="86"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51686"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96773.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="263" mass="30278" checksum="EBC3DE7B47E77EC2" modified="2013-06-26" version="1">MIRVNTLKISPEEVKERLEKKGVILEETFLDYAFKVKKSPFSIGATPEYLFGYYYLQSLSSMIPPIILNPKKRDFALDMCSAPGGKTTHMAQIMENQGVIVAVEISRERIKALKSNINRLGVLNTIVINADMREYKNYLLENNIFFDKILLDAPCSGNFIKDKNRNVSKKDILYCSIRQKELLNIGLDLLKKGGYLVYSTCSMEKEENEDVISYILNKREDIKLIKLKPKFKGINIKEGYIRGTLRVFPPDEPFFIAKIKKLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP53</accession>
    <name>N6VP53_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Flap endonuclease 1</fullName>
            <shortName evidence="12">FEN-1</shortName>
            <ecNumber evidence="12">3.1.-.-</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Flap structure-specific endonuclease 1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">fen</name>
        <name evidence="16" type="ORF">J422_06782</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.</text>
    </comment>
    <comment type="function">
        <text evidence="12">Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="12">Binds 2 magnesium ions per subunit. They probably participate in the reaction catalyzed by the enzyme. May bind an additional third magnesium ion after substrate binding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11 12">Interacts with PCNA. PCNA stimulates the nuclease activity without altering cleavage specificity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the XPG/RAD2 endonuclease family. FEN1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="12"/>
    <dbReference type="EMBL" id="APMM01000062">
        <property type="protein sequence ID" value="ENN95630.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594209.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000062.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP53"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01094"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1316"/>
    <dbReference type="OrthoDB" id="9593at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008409">
        <property type="term" value="F:5'-3' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017108">
        <property type="term" value="F:5'-flap endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09867">
        <property type="entry name" value="PIN_FEN1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000016">
        <property type="entry name" value="Flap endonuclease 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00614">
        <property type="entry name" value="Fen"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036279">
        <property type="entry name" value="5-3_exonuclease_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023426">
        <property type="entry name" value="Flap_endonuc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019973">
        <property type="entry name" value="Flap_endonuc_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008918">
        <property type="entry name" value="HhH2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006086">
        <property type="entry name" value="XPG-I_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006084">
        <property type="entry name" value="XPG/Rad2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019974">
        <property type="entry name" value="XPG_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006085">
        <property type="entry name" value="XPG_DNA_repair_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03674">
        <property type="entry name" value="fen_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081:SF9">
        <property type="entry name" value="FLAP ENDONUCLEASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11081">
        <property type="entry name" value="FLAP ENDONUCLEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00867">
        <property type="entry name" value="XPG_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00752">
        <property type="entry name" value="XPG_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00853">
        <property type="entry name" value="XPGRADSUPER"/>
    </dbReference>
    <dbReference type="SMART" id="SM00279">
        <property type="entry name" value="HhH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00484">
        <property type="entry name" value="XPGI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00485">
        <property type="entry name" value="XPGN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47807">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00841">
        <property type="entry name" value="XPG_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 12" id="KW-0227">DNA damage</keyword>
    <keyword evidence="9 12" id="KW-0234">DNA repair</keyword>
    <keyword evidence="1 12" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 12 16" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7 12" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 12" id="KW-0540">Nuclease</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="XPG N-terminal" evidence="15">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="domain" description="XPG-I" evidence="14">
        <location>
            <begin position="136"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="region of interest" description="N-domain" evidence="12">
        <location>
            <begin position="1"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Interaction with PCNA" evidence="12">
        <location>
            <begin position="312"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="83"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="150"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="219"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00024702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00065981"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="SMART" id="SM00484"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="SMART" id="SM00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN95630.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="320" mass="36718" checksum="F9DF615634AA193F" modified="2013-06-26" version="1">MGVPIGDYIPKKPISFEDLKGKKIAVDAMNILYQFLTSIRLKDGSPLKNKKGEITSAYNGLFYKTINLLEKGIIPIYVFDGEAPKLKERIREIRREMKEKAKEKLKEASDEEKVKYAKRIAYLNNNMVENCKYLLKLMGIPYVEAPSEGEAQASYMAKKGDVYAVLSQDYDSLLYGAPRVVRNLTTNDQPEIVELNEVLNTLRISLDDLIDLAIFLGTDYNPGGVKGIGFKRALEIIRSGMAKELLKKEVKEYDEIVNIFKNPKVTDNYSLNLRLPNKEGIIKFLVDEHDFNYERVKKHADRLYRIIEGMTKQKTLDAWF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP77</accession>
    <name>N6VP77_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Beta-ribofuranosylaminobenzene 5'-phosphate synthase</fullName>
            <shortName evidence="2">Beta-RFA-P synthase</shortName>
            <ecNumber evidence="2">2.4.2.54</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_06558</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the condensation of 4-aminobenzoate (pABA) with 5-phospho-alpha-D-ribose 1-diphosphate (PRPP) to produce beta-ribofuranosylaminobenzene 5'-phosphate (beta-RFA-P).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>5-phospho-alpha-D-ribose 1-diphosphate + 4-hydroxybenzoate + H(+) = 4-(beta-D-ribofuranosyl)phenol 5'-phosphate + CO2 + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:48556"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:17879"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="ChEBI" id="CHEBI:82767"/>
            <dbReference type="EC" id="2.4.2.54"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the beta-RFA-P synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.54" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000055">
        <property type="protein sequence ID" value="ENN95670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593714.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP77"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00607"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1276"/>
    <dbReference type="OrthoDB" id="85156at2157"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043793">
        <property type="term" value="F:beta-ribofuranosylaminobenzene 5'-phosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053442">
        <property type="entry name" value="Beta-RFA-P_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013750">
        <property type="entry name" value="GHMP_kinase_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006204">
        <property type="entry name" value="GHMP_kinase_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004422">
        <property type="entry name" value="RFAP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00144">
        <property type="entry name" value="beta_RFAP_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040726">
        <property type="entry name" value="BetaRFA-P_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861:SF6">
        <property type="entry name" value="BETA-RIBOFURANOSYLPHENOL 5'-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20861">
        <property type="entry name" value="HOMOSERINE/4-DIPHOSPHOCYTIDYL-2-C-METHYL-D-ERYTHRITOL KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08544">
        <property type="entry name" value="GHMP_kinases_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00288">
        <property type="entry name" value="GHMP_kinases_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004884">
        <property type="entry name" value="Sugar_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GHMP kinase N-terminal" evidence="3">
        <location>
            <begin position="68"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="domain" description="GHMP kinase C-terminal" evidence="4">
        <location>
            <begin position="228"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR004884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00288"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="325" mass="36330" checksum="8A0A1E7A9D69007D" modified="2013-06-26" version="1">MIIESPSRIHITLIDLNASIGRVDGGVGIALEKPNLKIEGDIERDIIIEFDNTLYNKFPEDFLNKIKNRAYNIAKDILNYIGKEGIYLKFLSLFPSHSGLGSGTQLSLAIGKLITKLYNYNIDIYEIAKLLGRGGTSGIGIGAFKYGGFLLDGGHSWKEKKDFKPSSASKGIRPAPILFRHNFDFDITLIIPEGEHVYGKREVDIFKKYCPIPLNEVEKLSHLILMKMLPAVVEKNLEDFGDVINKIQYLGFKKVEVGLQSEVVKELIQSLQKSAFSGLSSFGPTVYALGDKRFIIETANEVFDKFGVDGEIIFTKANNIGYKIW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP87</accession>
    <name>N6VP87_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Phosphosulfolactate synthase</fullName>
            <ecNumber evidence="2">4.4.1.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06331</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the phosphosulfolactate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.4.1.19" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95685.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593504.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP87"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01307"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1232"/>
    <dbReference type="OrthoDB" id="6405at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043817">
        <property type="term" value="F:phosphosulfolactate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019295">
        <property type="term" value="P:coenzyme M biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022370">
        <property type="entry name" value="Arch_ComA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003830">
        <property type="entry name" value="ComA_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036112">
        <property type="entry name" value="ComA_synth_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03849">
        <property type="entry name" value="arch_ComA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48413">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48413:SF1">
        <property type="entry name" value="PROTEIN HEAT-STRESS-ASSOCIATED 32"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02679">
        <property type="entry name" value="ComA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102110">
        <property type="entry name" value="(2r)-phospho-3-sulfolactate synthase ComA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="NCBIfam" id="TIGR03849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95685.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="253" mass="28775" checksum="75C6C7883906EB89" modified="2013-06-26" version="1">MKAFNFLLDEIERGLTVMLDKGLPPKFIESYLKVCGDYIDFVKFGWGTSAIMNKDVVKEKISYYNDYNIDVYPGGTLFECAVLKNKLDQFFEEALNLGFSAIEISDGSLDISLDLRKEAIKKARSYGLKVLTEVGKKLPEKDKELNINKRVEMIKFDLSAGADYVIIEGRESGRGIGIYDEKGNVKEDLLKAIEENVDINKLIFEAPNKNQQVYFILRYGPSVNLANISYEDVVSLETLRRGLRGDTFNKLQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPB2</accession>
    <name>N6VPB2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Type II secretion system F domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_06501</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95715.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593561.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPB2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01275"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1265"/>
    <dbReference type="OrthoDB" id="60490at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35007">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35007:SF2">
        <property type="entry name" value="PILUS ASSEMBLE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="30"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="56"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="198"/>
            <end position="222"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="242"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="272"/>
            <end position="290"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="93"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95715.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="299" mass="33618" checksum="16DAB49BB20B4C56" modified="2013-06-26" version="1">MNKYIRGFYYLFIKRNVLILRKLGKRINEIQFIFLLFILIIIFILLGSLLHLTSRSILLLIIVYFGAILHLPKILYENRIELLEKNIPKALYIMVISLESGRSIPEALQEVVKNNIKEVSDVFRKVLYLMENQKLTFEESISIVATIYDSKVLSMLSRIMIENKKYGGDLAESLKNLAKTLEDLQLYKRQLLSVTAQGLAIGFIILCGVMPAVGGLLGAYLLFITKSMGGMARTPPVSAEDIARGFEIIQLGTLMVGSLFSIPIYGFNIARMFLIAAITMTAGMGAYYIILKLAPSLFS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPG1</accession>
    <name>N6VPG1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05933</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95775.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPG1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00573"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1155"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009573">
        <property type="entry name" value="HcgC"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06690">
        <property type="entry name" value="HcgC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95775.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="260" mass="29660" checksum="94EE6B805D3A10F5" modified="2013-06-26" version="1">MTGITENIKTLETKTRVIDVINSISKKKYNAMKMFLEGEEFKEAVVFGVYLWGNYVAQNIKNFCDKVYLVDIYEFLKDLIPNDNIEFLTLNDFKLKLIKDKINPDLVIDITGLGGIDPNFLKKFSPKIAIIEDPRGVFDPDIYELDNTYKRAYSINSEKVGILKTFRKAKISKTSGTMTLTIDTIVDASKEISSLDGVLYAIPNLKYYEGILFHNGNIKRFLLELSKPAIIISSINDVINEAEKIIENNLNLIYSFIDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPZ4</accession>
    <name>N6VPZ4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein uL13</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">rpl13</name>
        <name evidence="6" type="ORF">J422_04965</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">This protein is one of the early assembly proteins of the 50S ribosomal subunit, although it is not seen to bind rRNA by itself. It is important during the early stages of 50S assembly.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4 5">Belongs to the universal ribosomal protein uL13 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95965.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592101.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPZ4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00497"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.973"/>
    <dbReference type="OrthoDB" id="7668at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003729">
        <property type="term" value="F:mRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017148">
        <property type="term" value="P:negative regulation of translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00392">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1180.10">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01366">
        <property type="entry name" value="Ribosomal_uL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005822">
        <property type="entry name" value="Ribosomal_uL13"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005823">
        <property type="entry name" value="Ribosomal_uL13_bac-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023563">
        <property type="entry name" value="Ribosomal_uL13_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005755">
        <property type="entry name" value="Ribosomal_uL13_euk/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036899">
        <property type="entry name" value="Ribosomal_uL13_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01077">
        <property type="entry name" value="L13_A_E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005004">
        <property type="entry name" value="PRK06394.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11545">
        <property type="entry name" value="RIBOSOMAL PROTEIN L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00572">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002181">
        <property type="entry name" value="Ribosomal_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52161">
        <property type="entry name" value="Ribosomal protein L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00783">
        <property type="entry name" value="RIBOSOMAL_L13"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 5" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01366"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="RuleBase" id="RU003877"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95965.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="137" mass="15799" checksum="A2BA9ED8C4D3144C" modified="2013-06-26" version="1">MTVIDAEGAILGRLASTVAKRVLRGEEITIINAEKVIITGNRDWIIKHYQEERAKKNVANPRRFGPKFPRRPDDILRRTIRKMLPYKKPKGREAFKRVKVYVGNPLNLQPDEKISHELKTNKFITLGELSKHLGAKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQG4</accession>
    <name>N6VQG4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Aspartate--tRNA(Asp/Asn) ligase</fullName>
            <ecNumber evidence="11">6.1.1.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Aspartyl-tRNA synthetase</fullName>
            <shortName evidence="11">AspRS</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Non-discriminating aspartyl-tRNA synthetase</fullName>
            <shortName evidence="11">ND-AspRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">aspC</name>
        <name evidence="11" type="synonym">aspS</name>
        <name evidence="13" type="ORF">J422_04113</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>tRNA(Asx) + L-aspartate + ATP = L-aspartyl-tRNA(Asx) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18349"/>
            <dbReference type="Rhea" id="RHEA-COMP:9710"/>
            <dbReference type="Rhea" id="RHEA-COMP:9711"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78516"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 3 Mg(2+) cations per subunit. The strongest magnesium site (Mg1) is bound to the beta- and gamma-phosphates of ATP and four water molecules complete its coordination sphere.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-II aminoacyl-tRNA synthetase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.23" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000027">
        <property type="protein sequence ID" value="ENN96115.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591618.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQG4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01352"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.805"/>
    <dbReference type="OrthoDB" id="5908at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004815">
        <property type="term" value="F:aspartate-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050560">
        <property type="term" value="F:aspartate-tRNA(Asn) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006422">
        <property type="term" value="P:aspartyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00776">
        <property type="entry name" value="AsxRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd04316">
        <property type="entry name" value="ND_PkAspRS_like_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000038">
        <property type="entry name" value="Aspartate--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000324">
        <property type="entry name" value="Aspartate--tRNA(Asp/Asn) ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02075">
        <property type="entry name" value="Asp_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004364">
        <property type="entry name" value="Aa-tRNA-synt_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004523">
        <property type="entry name" value="Asp-tRNA_synthase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002312">
        <property type="entry name" value="Asp/Asn-tRNA-synth_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00458">
        <property type="entry name" value="aspS_nondisc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003483">
        <property type="entry name" value="PRK05159.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450:SF1">
        <property type="entry name" value="ASPARTATE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43450">
        <property type="entry name" value="ASPARTYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00152">
        <property type="entry name" value="tRNA-synt_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01042">
        <property type="entry name" value="TRNASYNTHASP"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="12">
        <location>
            <begin position="103"/>
            <end position="428"/>
        </location>
    </feature>
    <feature type="region of interest" description="Aspartate" evidence="11">
        <location>
            <begin position="191"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="169"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="213"/>
            <end position="215"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="221"/>
            <end position="223"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="351"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="354"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="399"/>
            <end position="402"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Important for tRNA non-discrimination" evidence="11">
        <location>
            <position position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005312"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02075"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96115.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="428" mass="49110" checksum="2A00F1F576AEED14" modified="2013-06-26" version="1">MRTHYSSELSANMDGEIVKVAGWVHSIRALGKLIFVILRDREGFIQIVVPKQKVGEDLFKKIKSLGVEDVISVKGKVVANEKAPNGFEILPLEVDVLNTAKRPLPLDPAEKVPAELDTRLENRFLDLRKPKIQAIFKIRHEMLKSVRNTFYEEGFIEVHTPKLVASCTEGGTELFPISYFEKEAFLSQSPQLYKQMLMATGLDRVFEIAPIFRAEEHNTRRHLNEAISIDVEMAFADDVEAIKMLEKVVHNAIKDVLKNREKELELLGVELELPKKFDKITYDEAIEIAKKKGVEIDWGEDLSREAEKAIGEEMGGFYFIVDWPSDIRPFYTMPKDDRYCKAFDLMYKDLELASGAQRIHLYDLLVENIKKKGLNPDAFTYYLEAFKYGMPPHAGWGLGAERFTMVLTGRENIRECALFPRDRHRLTP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQK0</accession>
    <name>N6VQK0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Damage-control phosphatase ARMT1-like metal-binding domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03883</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96165.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591378.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQK0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00407"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.761"/>
    <dbReference type="OrthoDB" id="359165at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.380">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.285.20">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10880">
        <property type="entry name" value="Uncharacterised protein PF01937, DUF89, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036075">
        <property type="entry name" value="ARMT-1-like_metal-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002791">
        <property type="entry name" value="ARMT1-like_metal-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014444">
        <property type="entry name" value="PH1575-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01937">
        <property type="entry name" value="ARMT1-like_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006593">
        <property type="entry name" value="UCP006593"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111321">
        <property type="entry name" value="AF1104-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Damage-control phosphatase ARMT1-like metal-binding" evidence="1">
        <location>
            <begin position="4"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96165.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="287" mass="32462" checksum="185687D353682120" modified="2013-06-26" version="1">MKIKPECAICIVRQVVDASKEITDIEEKQFYLIKETLKVIQEVYSPSTVPAWMGTVVHRYLKKISGCKDPYKRLKEKANDVALKYLKYAKEFCNVDNDLERLKRKIILSIAGNVIDYGAYSKDINVEEMLKKTLDEGLKIDHTLNLMEDLKNNKINNVLYICDNAGEIVFDKLLIDEIKSYGKNVVAVVKGEPILNDATLEDAKIAGIENVITTGSDVIGVILEECSREFLDHFNKADIIISKGMGNYESLTEYKLDKPIYFILKAKCKPVADHLGVNVGDNVCKKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQT3</accession>
    <name>N6VQT3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Coenzyme gamma-F420-2:alpha-L-glutamate ligase</fullName>
            <ecNumber evidence="4">6.3.2.32</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_03328</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.32" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96260.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590967.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQT3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01052"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.652"/>
    <dbReference type="OrthoDB" id="33241at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043774">
        <property type="term" value="F:coenzyme F420-2 alpha-glutamyl ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013651">
        <property type="entry name" value="ATP-grasp_RimK-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031039">
        <property type="entry name" value="F420_CofF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004666">
        <property type="entry name" value="Rp_bS6_RimK/Lys_biosynth_LsyX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04443">
        <property type="entry name" value="F420_CofF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00768">
        <property type="entry name" value="rimK_fam"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621:SF2">
        <property type="entry name" value="COENZYME GAMMA-F420-2:ALPHA-L-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21621">
        <property type="entry name" value="RIBOSOMAL PROTEIN S6 MODIFICATION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08443">
        <property type="entry name" value="RimK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0436">Ligase</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="6">
        <location>
            <begin position="98"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="NCBIfam" id="TIGR04443"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96260.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="285" mass="32655" checksum="0CC7DC13C8F727EE" modified="2013-06-26" version="1">MVKITILSPEAKGRTVWALKEEIEKLNAKAEIFLLAENDNLITYDFSLETDLIHSRCGIGFYNDKLTLYSWQVMNSLDAKFVNSLETVYLTSDKFKTIRILHKNNILTPKTALIRDYDDAIKFMEKYGLEFPVVIKKSFSKCGVSVFLAKNEEELWRLTKDAIYECKIIQEFINFKNNGYYKDIRVLVVDDEIVGGYSRVSKDFRTNIHLGNNVEKINLNDEVKEIALKCAEISKAKILGVDILPKMGKYYVIELNSAPGTEGFRKLGVNADKKIAELLVREAKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQX2</accession>
    <name>N6VQX2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">6-carboxyhexanoate--CoA ligase</fullName>
            <ecNumber evidence="8 9">6.2.1.14</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Pimeloyl-CoA synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">bioW</name>
        <name evidence="10" type="ORF">J422_03034</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the transformation of pimelate into pimeloyl-CoA with concomitant hydrolysis of ATP to AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>heptanedioate + ATP + CoA = 6-carboxyhexanoyl-CoA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14781"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:36165"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57360"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.2.1.14"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="8">Metabolic intermediate metabolism; pimeloyl-CoA biosynthesis; pimeloyl-CoA from pimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the BioW family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.14" evidence="8 9"/>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96300.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590751.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQX2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00154"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.595"/>
    <dbReference type="OrthoDB" id="65815at2157"/>
    <dbReference type="UniPathway" id="UPA00999">
        <property type="reaction ID" value="UER00351"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042410">
        <property type="term" value="F:6-carboxyhexanoate-CoA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00668">
        <property type="entry name" value="BioW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005499">
        <property type="entry name" value="BioW"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01204">
        <property type="entry name" value="bioW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002360">
        <property type="entry name" value="PRK01322.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03744">
        <property type="entry name" value="BioW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 8" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="3 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="7 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="NCBIfam" id="TIGR01204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96300.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="228" mass="26343" checksum="580C2C6DAF0B8F4C" modified="2013-06-26" version="1">MFSIKMRASLNNKHISGAERIVKKNKIKEVSLELINRALNHENGEPDFINIKIEKLKEVRYIKHLPIVTIECKNKIEARSRAKEILLKEKIPEHVIDKAFNIIDKGGMRGAAILDFEGNRLDPDKERGVRVKNIDVTDKLREEIIRKYTERTADAIAIATKVIHLGIIAELCTSDNRSYTTGYVATKNGYFRITNLKNKNEPGGRVFFVKNFDYELIRRLENEAYIIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR54</accession>
    <name>N6VR54_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Orotate phosphoribosyltransferase</fullName>
            <shortName evidence="7">OPRT</shortName>
            <shortName evidence="7">OPRTase</shortName>
            <ecNumber evidence="2 7">2.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pyrE</name>
        <name evidence="9" type="ORF">J422_02919</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10380"/>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57538"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.10" evidence="2 7"/>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96390.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590661.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR54"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00132"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.573"/>
    <dbReference type="OrthoDB" id="9089at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00119"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.2020:FF:000029">
        <property type="entry name" value="Orotate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01208">
        <property type="entry name" value="PyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023031">
        <property type="entry name" value="OPRT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004467">
        <property type="entry name" value="Or_phspho_trans_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00336">
        <property type="entry name" value="pyrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF9">
        <property type="entry name" value="URIDINE 5'-MONOPHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7 9" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="5 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="8">
        <location>
            <begin position="40"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="7">
        <location>
            <begin position="112"/>
            <end position="120"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="116"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="144"/>
        </location>
        <ligand>
            <name>orotate</name>
            <dbReference type="ChEBI" id="CHEBI:30839"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004889"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01208"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96390.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="176" mass="19508" checksum="82F4F96E751DF18A" modified="2013-06-26" version="1">MKEELIRLLKSEGCIKFGDFILSSGKRSNYYVDIKKAISNPKILKIIGKMIAEKIEDEKVAGIELGSVPIATAVSLYAEKPLIIIRKKPKDYGTKSKIEGHVEKGDKVVIVEDVTTTGNSVLKAVKELRNAGAIVEKVFVVVDRNEGAKENLKKEGVELIPLITVEELYDKQKNNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRA1</accession>
    <name>N6VRA1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Formate dehydrogenase family accessory protein FdhD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02355</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96430.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590342.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRA1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00352"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.463"/>
    <dbReference type="OrthoDB" id="57189at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016783">
        <property type="term" value="F:sulfurtransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.140.10">
        <property type="entry name" value="Cytidine Deaminase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016193">
        <property type="entry name" value="Cytidine_deaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003786">
        <property type="entry name" value="FdhD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592">
        <property type="entry name" value="FORMATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30592:SF1">
        <property type="entry name" value="SULFUR CARRIER PROTEIN FDHD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02634">
        <property type="entry name" value="FdhD-NarQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53927">
        <property type="entry name" value="Cytidine deaminase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96430.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="210" mass="23984" checksum="09308B031708638A" modified="2013-06-26" version="1">MITKIKIINSNLNKEEDIIAIEEEYELFINNKFIGTFSISPSHIKEFAIGHAISEGYVNEIDKVLVDNEKILVFGSKKIRDLCIKKPNIDVIKRVINYNLELKYWKLTGCFHWASIFNNNGKFITLIEDISRSCAVDKVIGYAYLNNYNNIILKFSGRIFKSIVKKAANSGIKVIISKSPATDKAIKLAKERDITLIGFAREGRFNIYNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRH7</accession>
    <name>N6VRH7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA double-strand break repair protein Mre11</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">mre11</name>
        <name evidence="12" type="ORF">J422_01685</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Part of the Rad50/Mre11 complex, which is involved in the early steps of DNA double-strand break (DSB) repair. The complex may facilitate opening of the processed DNA ends to aid in the recruitment of HerA and NurA. Mre11 binds to DSB ends and has both double-stranded 3'-5' exonuclease activity and single-stranded endonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="9">Binds 2 manganese ions per subunit.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">Nuclease activity is regulated by Rad50.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer. Forms a heterotetramer composed of two Mre11 subunits and two Rad50 subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the MRE11/RAD32 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96490.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590095.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRH7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00188"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.331"/>
    <dbReference type="OrthoDB" id="11638at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008408">
        <property type="term" value="F:3'-5' exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045027">
        <property type="term" value="F:DNA end binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030145">
        <property type="term" value="F:manganese ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000403">
        <property type="term" value="F:Y-form DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00840">
        <property type="entry name" value="MPP_Mre11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.600">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.10.50">
        <property type="entry name" value="Mre11, C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02044">
        <property type="entry name" value="Mre11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050535">
        <property type="entry name" value="DNA_Repair-Maintenance_Comp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032885">
        <property type="entry name" value="Mre11_archaea-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055244">
        <property type="entry name" value="Mre11_C_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041796">
        <property type="entry name" value="Mre11_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337">
        <property type="entry name" value="COMPONENT OF ATP-DEPENDENT DSDNA EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30337:SF0">
        <property type="entry name" value="NUCLEASE SBCCD SUBUNIT D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22226">
        <property type="entry name" value="Mre11_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7 9" id="KW-0234">DNA repair</keyword>
    <keyword evidence="3 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8 9" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="10">
        <location>
            <begin position="1"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="DNA double-strand break repair protein Mre11 C-terminal Rad50-binding" evidence="11">
        <location>
            <begin position="315"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="9">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="49"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="184"/>
        </location>
        <ligand>
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF22226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96490.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="358" mass="42374" checksum="E3BB912DFE9B4192" modified="2013-06-26" version="1">MQFVHIADNHLGYRHYNLDEREMDIYENFNECIDKIIEIKPDFVIHSGDLFNDLRPQIEALRIAIEGFRKLKDMGIRVYIVAGNHEMPRRRNRKSPIILLKDYVKILNGYVLEGDIFIAGTYYHNLNKKEELRNKIKEFEIKGAGYKKKILALHQGINKYLPYDYELEINELPSFSYYALGHVHNRVLERFNDGILAYAGSTEIIRIDEYESYKKDGKGFYLVDFSKGDIDISDVDKINLECRDIIKAEIKTEKDLLEVIKEINNAKKKPILIGKIKEELRHRLDIIKDKILINKLIPVEEKIFDEIDTVETLDIKELLIKYANNNKLNGEFVYLLYRKLINNEDWRDIVDNFYKNFM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRJ0</accession>
    <name>N6VRJ0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">PP-loop domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05938</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95776.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593033.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRJ0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00572"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1156"/>
    <dbReference type="OrthoDB" id="10500at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012121">
        <property type="entry name" value="ATPase_PP-loop_MJ1599"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052188">
        <property type="entry name" value="Ni-pincer_cofactor_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169:SF3">
        <property type="entry name" value="ATPASE, PP-LOOP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169">
        <property type="entry name" value="EXSB FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036668">
        <property type="entry name" value="ATPase_UCP036668"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="1">
        <location>
            <begin position="59"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95776.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="280" mass="32962" checksum="A85C3A4B15841941" modified="2013-06-26" version="1">MLECSICVHTEKTKKILFEDKPICVDCLTYKKYPIDFNKLKKEVEETLEKIKKENKKYQCILAFSGGKDSVVALKLLKEEFKINPICVLVDNNYLSEDVIKNAINVTKYYNTDLIIINRDFTWLFNEVLNRCESPCRRCSRLILREVWRVAKLLGIKYIITGHELPFGHSAIRDMKDGIKMIRLLAPYKIREEEKYELIKDLPWKKPNLYGYTTNCLILGVALERFYQKYGFSFEIDRIAAMVRLGLLNKEKAKEFLKKPNIPEELYEELKSRGLKLINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRK5</accession>
    <name>N6VRK5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Lipopolysaccharide biosynthesis protein RfbH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_05898</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3 4">Belongs to the DegT/DnrJ/EryC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000046">
        <property type="protein sequence ID" value="ENN95791.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592962.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRK5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00992"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1150"/>
    <dbReference type="OrthoDB" id="10355at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000271">
        <property type="term" value="P:polysaccharide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00616">
        <property type="entry name" value="AHBA_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000079">
        <property type="entry name" value="LPS biosynthesis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000653">
        <property type="entry name" value="DegT/StrS_aminotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011936">
        <property type="entry name" value="PRK15407.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244:SF34">
        <property type="entry name" value="DTDP-4-AMINO-4,6-DIDEOXYGALACTOSE TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30244">
        <property type="entry name" value="TRANSAMINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01041">
        <property type="entry name" value="DegT_DnrJ_EryC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000390">
        <property type="entry name" value="PLP_StrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00037999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU004508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95791.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="434" mass="49709" checksum="0FCB0A0FB0C69335" modified="2013-06-26" version="1">MPEGIKNKILELVKEYYYKTYKKLPYNKVPVSGKVYDEKELQNIVEAALEGWWTEGKWCSIFEEKLKNFLNIDFALLVNSGSSANFIAIKTLTSIKLGDRKIKPGDEVITVAAGFPTTVNPIIEVGAIPVFVDVELGTYNAIVEQIQEAVSPKTKAIFLAHTLGNPFDVKAIKEICEDYNLWLIEDNCDALGSKYDGRYTGTFGDLATLSFYPAHHITTAEGGAVLTNDPQLYRIARSIRDWGRDCWCPTGKDNTCGRRFSWQLGKLPYGYDHKYIYSEIGYNLKMTDLQAAIGVAQMDKLGYFIKKRKENFEYLYNKLKEFEDYFILPKATENSEPSWFGFLLTIKDSSNINRTKFMQYLNDNGIGTRLLFGGNLIKQPYFVDYGIKYKQIRNLENTDIVMNNTFWIGVYPALEKGHLDYVYEVFKKYLDGVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRM9</accession>
    <name>N6VRM9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01915</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96530.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590141.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRM9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00231"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.377"/>
    <dbReference type="OrthoDB" id="65838at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96530.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="145" mass="16228" checksum="D0BB63BFA294B55B" modified="2013-06-26" version="1">MRGQISIDLILAFMMIMVISIFLYNNIINFSDSLISGVLADKVYEVANKIENYALLAYSNNNSLAVVLKPIGEENYVIYVANYTINVTHPITIILTPTKDGVMIKGNINYSVNNTIYGFNLSKGNIILIEYSEGYIAKNTSILFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRN4</accession>
    <name>N6VRN4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Glutamyl-tRNA(Gln) amidotransferase subunit E</fullName>
            <shortName evidence="6">Glu-ADT subunit E</shortName>
            <ecNumber evidence="6">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">gatE</name>
        <name evidence="8" type="ORF">J422_01940</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GatB/GatE family. GatE subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96535.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590152.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRN4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00236"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.382"/>
    <dbReference type="OrthoDB" id="7316at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004812">
        <property type="term" value="F:aminoacyl-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070681">
        <property type="term" value="P:glutaminyl-tRNAGln biosynthesis via transamidation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.410:FF:000003">
        <property type="entry name" value="Glutamyl-tRNA(Gln) amidotransferase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1360.30:FF:000003">
        <property type="entry name" value="Glutamyl-tRNA(Gln) amidotransferase subunit E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.30">
        <property type="entry name" value="GAD-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.380">
        <property type="entry name" value="GatB domain, N-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00588">
        <property type="entry name" value="GatE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017959">
        <property type="entry name" value="Asn/Gln-tRNA_amidoTrfase_suB/E"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006075">
        <property type="entry name" value="Asn/Gln-tRNA_Trfase_suB/E_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018027">
        <property type="entry name" value="Asn/Gln_amidotransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003789">
        <property type="entry name" value="Asn/Gln_tRNA_amidoTrase-B-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004115">
        <property type="entry name" value="GAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029351">
        <property type="entry name" value="GAD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042114">
        <property type="entry name" value="GatB_C_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023168">
        <property type="entry name" value="GatB_Yqey_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004414">
        <property type="entry name" value="GatE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017958">
        <property type="entry name" value="Gln-tRNA_amidoTrfase_suB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014746">
        <property type="entry name" value="Gln_synth/guanido_kin_cat_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00134">
        <property type="entry name" value="gatE_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003107">
        <property type="entry name" value="PRK04028.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659">
        <property type="entry name" value="GLUTAMYL-TRNA GLN AMIDOTRANSFERASE SUBUNIT B MITOCHONDRIAL AND PROKARYOTIC PET112-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11659:SF2">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02938">
        <property type="entry name" value="GAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02934">
        <property type="entry name" value="GatB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02637">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00845">
        <property type="entry name" value="GatB_Yqey"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55261">
        <property type="entry name" value="GAD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89095">
        <property type="entry name" value="GatB/YqeY motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55931">
        <property type="entry name" value="Glutamine synthetase/guanido kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01234">
        <property type="entry name" value="GATB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Asn/Gln amidotransferase" evidence="7">
        <location>
            <begin position="472"/>
            <end position="615"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00047913"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00588"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00845"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96535.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="619" mass="70721" checksum="F924E704E86BD8A5" modified="2013-06-26" version="1">MDYNEIGLKVGLEIHQQLNTERKLFCHCPTELTNEVKGEVERILRATLSELGEIDRAALLEMRKGKKFIYQYNNSSCLVELDEEPPHMPSEEALKIALQIAMLLNMKTVDVAYVMRKIVIDGSNPSGFQRTIFLAYDGYVETEDGKVRIESLCLEEDAARKVEERGDKVVYNLDRLGIPLVEISTAPDINSPKMARETAKRIGEILRMCKVKRGIGTIRQDINISIKDGARVEIKGVSELYLIEKVVEYEVLRQLNLLKIRDELKERNAEVIEEIYDVTDIFKNCNSKIIKRALKDGKVKAIVLKKFGGLVGKEIQPGRRLGTEFADRAKVIAGVSGIIHTDELPKYGITEEDVKRLREFLKVDEDDAIVLVADKEEKANKALQAVIERAKEALIGVPEETRKANEDGTTSYLRPLPGKARMYPETDIPPIFIKDIEVERPELPEEKIERFKREYMLSDELAKKMVFSYYVDLFEELCKRFKNIKPTLIAKTLEESLKEIKREGYNIENIKKQHLYSLFDALSSGKLAKEGIIEVLKGFCEMPDKDIDEILEIKGLKGLSREEVEKIVEEIINENINIIKEKKDKALGYLMGRCMAKLRGKADGKMVNEILKEKIRNIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRX0</accession>
    <name>N6VRX0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TPR repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_01413</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96615.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589944.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRX0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00006"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.277"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13432">
        <property type="entry name" value="TPR_16"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="3"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="149"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96615.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="285" mass="33085" checksum="7DA9E8FF8C9F7229" modified="2013-06-26" version="1">MNINELILNAIKSIDYGDTESAKKFIEKALELDPNNTLAKYIRDKIYDLEPHMGCCNFMLGNFERAAYTKDTFPEETHICFLQPYRRAMALVELGEYDKALKALDDLIKVKKDFVPAWRQKAVLLETLGRYEEALKCIDVILSLNSFDIFALYLKGKILKQLGRFKEALEYLYKAVNLNKDLILAYKDIAYCELIIGNYRNALEWLNKYLEKVPNDNEALFYLALAYEGLKEDEKAIKILDKVIKRAKNKNLLLYKNAILLKAKILERNGKLDEAIELYNKALEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRX6</accession>
    <name>N6VRX6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01258</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589915.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRX6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00038"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.246"/>
    <dbReference type="OrthoDB" id="358798at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1360">
        <property type="entry name" value="mj0159-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002846">
        <property type="entry name" value="NRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038982">
        <property type="entry name" value="NrpR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036984">
        <property type="entry name" value="NrpR_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013668">
        <property type="entry name" value="RNase_R_HTH_12"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41964">
        <property type="entry name" value="GLOBAL NITROGEN REGULATOR NRPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41964:SF1">
        <property type="entry name" value="GLOBAL NITROGEN REGULATOR NRPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01995">
        <property type="entry name" value="NRD1_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08461">
        <property type="entry name" value="WHD_RNase_R"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ribonuclease R winged-helix" evidence="2">
        <location>
            <begin position="8"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="NrpR regulatory" evidence="1">
        <location>
            <begin position="83"/>
            <end position="300"/>
        </location>
    </feature>
    <feature type="domain" description="NrpR regulatory" evidence="1">
        <location>
            <begin position="318"/>
            <end position="532"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01995"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF08461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="538" mass="61425" checksum="386836EE0F1DE82D" modified="2013-06-26" version="1">MDLDRKLIEILDILSKYKEPVGATIIAKELSKRGYKIGERAVRYHLKILDSLGLTKKVGYVGRIITEKGLEELRKADVFYRLGSIYSNILEKMLSFNYYDRYAIVNRAYIYADFNVVIKEIKKIYNSGFAVGDRVGIVDKGKYVELYTLCSLNFDNILIKNGILPTHVCGGIVKYEDGEPVKFEEIIYYKATSIDPLKVFIKEKKTDVVGILEEGNGYLPVNFRYIPKSSMEKFERILKNDELKCVISYGEENVLGLKVDDDSIGIALIGGLSPIAVFTEKGYYADIQPMELLVKIDSLHKLEKNERKIVKKKSNYKIKTIFYKMVNAMSMVDYDIDKHDGKVIVNICYIDKKYADDALDLLKEAYKNNLGIGDKFGFIEEKDKIKIQTLCSITIDGILLKHSLPTIPKYGGVLEIKNEKKRFIDIIAYNGSSLDPHIVFFDAVDCESTILAGFREFHRIIKDDIEEVLDRLNWNCVIGIGEPNNEIFGINVEKDMCGIVYNGGLNPIVLLKENEIPVEIKQMHEVVDYSKLISYKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS06</accession>
    <name>N6VS06_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Lon proteolytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_04845</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Endomembrane system</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the peptidase S16 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95941.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592053.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS06"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00521"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.949"/>
    <dbReference type="OrthoDB" id="15525at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012505">
        <property type="term" value="C:endomembrane system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004252">
        <property type="term" value="F:serine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030163">
        <property type="term" value="P:protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008269">
        <property type="entry name" value="Lon_proteolytic"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027065">
        <property type="entry name" value="Lon_Prtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10046">
        <property type="entry name" value="ATP DEPENDENT LON PROTEASE FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05362">
        <property type="entry name" value="Lon_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00830">
        <property type="entry name" value="ENDOLAPTASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51786">
        <property type="entry name" value="LON_PROTEOLYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0720">Serine protease</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="558"/>
            <end position="577"/>
        </location>
    </feature>
    <feature type="domain" description="Lon proteolytic" evidence="4">
        <location>
            <begin position="15"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="100"/>
        </location>
    </feature>
    <feature type="active site" evidence="2">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95941.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="582" mass="65626" checksum="18A3AFF90B34602C" modified="2013-06-26" version="1">MRKLILLLLLINPVLAVVIKAPAVSITDHGFVGVPITFEIHAQKGDGHVFIDTMPLTQLDMQSSARIAAKVAGEIANKDIDNYNVYIIVRSDVPIVGGPSAGGTMTIGILAELLNLTINKNVMMTGTINPDGSIGPVGGILEKIEAAKEANCSIFLIPKGQRYIKEGDREVDAVEFGKRLGIEVVEVGSIYEAFPYFFNKKITIKSYPPNPIAEEKYKRIMKSLAEKILNMSEEKYRNISTILEQDYFGYEYNYLLNCKLNEAKEMIEKANREYINKKYYSATSTAFNALIKLEDIENKIKFLSGVSVKSILLKIQDDISKDKEIVYSKNLTTKNFEEVLSARLRIKKAESLLDEAWKEYYLGNYDKAIEYGSFADMRGKSAIWWISLAEEGGEKIDNNKLKVLAQQYLDNAQTVLTYLETLYPNINFDDLENELKNAKEAYNEGDYILSIAQSIDLSVKAEVPLVIFSDIEYLKNYAKNKINLAERYITPISALSYYEYAETLEDNVSKIMYYKYSSYCAQMDLDILKILNKSVIVKNPEGNLYKEVKEKPELDETAIIITAVLSGLIGFSAGYIFRKVTA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS10</accession>
    <name>N6VS10_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">H(2)-dependent methylenetetrahydromethanopterin dehydrogenase-related protein</fullName>
            <ecNumber evidence="5">1.12.98.2</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_01196</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the HMD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.12.98.2" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589905.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS10"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00805"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.234"/>
    <dbReference type="OrthoDB" id="70340at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047068">
        <property type="term" value="F:N5,N10-methenyltetrahydromethanopterin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1300">
        <property type="entry name" value="Hmd, C-terminal helical subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004889">
        <property type="entry name" value="HMD_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038182">
        <property type="entry name" value="HMD_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055205">
        <property type="entry name" value="HMD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010063">
        <property type="entry name" value="HMDII/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024190">
        <property type="entry name" value="METHMP_Hmd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01724">
        <property type="entry name" value="hmd_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009208">
        <property type="entry name" value="PRK12557.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03201">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22616">
        <property type="entry name" value="HMD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016158">
        <property type="entry name" value="HMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500166">
        <property type="entry name" value="HMDII_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="5,10-methenyltetrahydromethanopterin hydrogenase N-terminal" evidence="4">
        <location>
            <begin position="65"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="H2-forming N5,N10-methylenetetrahydromethanopterin dehydrogenase C-terminal" evidence="3">
        <location>
            <begin position="194"/>
            <end position="289"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008890"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03201"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="342" mass="37642" checksum="7C8DDC9476936D24" modified="2013-06-26" version="1">MKISVYGAGNQRLYIDKLKLHKKFNGKPPYGGAKIAMEFAEVGHDVILSEPNRNVMKEELWKCVKDVGVKVVNDDIEAAKHGEVHILFTPFGNHTIKIAEKIVRNVPKDAIICTTCTLSPLVLYYSLEKILKFERRDVGISSMHPASVPGTELHKHYVISGKTLDGKELCREEQINKLVELCKSVNKKPYLIPAGVSSALADMGSLVTAVALAGILDYYTVGRKIINAPKKMIEHQVMMSLYVLASIVETSGIDGLLKAIDLDLLINSASSMKLIDDQKDLLSALEILKNLKNDILEESKNAELKETCFVASQSLVKEIKSLIGGAAAEGAIKRSTIKLFKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS44</accession>
    <name>N6VS44_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 7">Replication factor C large subunit</fullName>
            <shortName evidence="7">RFC large subunit</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6 7">Clamp loader large subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rfcL</name>
        <name evidence="10" type="ORF">J422_00601</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heteromultimer composed of small subunits (RfcS) and large subunits (RfcL).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the activator 1 small subunits family. RfcL subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96700.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589800.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS44"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00913"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.115"/>
    <dbReference type="OrthoDB" id="8658at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01508">
        <property type="entry name" value="RfcL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023935">
        <property type="entry name" value="Rep_factor-C_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003229">
        <property type="entry name" value="PRK04195.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003230">
        <property type="entry name" value="PRK04195.1-6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389">
        <property type="entry name" value="CHROMOSOME TRANSMISSION FIDELITY FACTOR 18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23389:SF6">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21960">
        <property type="entry name" value="RCF1-5-like_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="9">
        <location>
            <begin position="37"/>
            <end position="157"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="414"/>
            <end position="465"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="8">
        <location>
            <begin position="418"/>
            <end position="456"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="45"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00032141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96700.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="465" mass="53302" checksum="870E0BD2404EE6AA" modified="2013-06-26" version="1">MNWIEKYRPKSLKDVVGHEKVKERLRNWIESYIRGEKQKPILLYGPPGCGKTTMAYALANDYNFDVIELNASDNRSASIIKRIVGQSAVSSSIFGKRFLVILDEVDGISGKEDAGGVGEIIKIIKKAKNPIILTANDAYAPSLRNLLQHVEVIELKPLTTTQILNVLKKIAEKENLNVDEKTLRFIAQRSAGDLRSAINDLEALALSNDLSYEAAQTLADRNREANIFDALRVILKTTKYGIASMALMNVDETPDVIIEWIAENVPKEYEKIEEIEKAFDYLSKADMFLGRAVRKQNYSFWKYAGTLMTAGVALAKDEKYRKFTPYSYPKIFRLLSKTKADREIIKKILKKIGEKTHCSTKRARFDLEALKIIAKENKELAADLVKYFEIKEDELKALLGEELASEIIKIIKKRKKEDKKIKKKEEVKKEKEAKKETKEEKKEENKKEKEKKEKPKQMTLDVFFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS73</accession>
    <name>N6VS73_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04640</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96001.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591953.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS73"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00946"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.907"/>
    <dbReference type="OrthoDB" id="359190at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96001.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="125" mass="14745" checksum="AF40B02C93280F37" modified="2013-06-26" version="1">MERNITTKILDNTVISAGLKEIKTIDLIERCLKRYKLATSKHVFEETLKGFSNSTIKNCYSDIKVHETDEETLNFLLDYLEKRFPYLHRGELSSFLVALLKYAEKGKPYYYVTDDNRIRNKIPKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSB7</accession>
    <name>N6VSB7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00371</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96770.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589756.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSB7"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.71"/>
    <dbReference type="OrthoDB" id="102288at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96770.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="45" mass="5393" checksum="34EB57D85CBA7012" modified="2013-06-26" version="1">MVRIDLKELDKYVKEFKCKCGYTFRYVGSSIICPKCKTLWENVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSG7</accession>
    <name>N6VSG7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0147 protein J422_00185</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00185</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0147 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96820.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589726.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSG7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01131"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.37"/>
    <dbReference type="OrthoDB" id="65304at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1440.50">
        <property type="entry name" value="Ta0600-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00342">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023130">
        <property type="entry name" value="Ta0600-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005354">
        <property type="entry name" value="UPF0147"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003319">
        <property type="entry name" value="PRK04330.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03685">
        <property type="entry name" value="UPF0147"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158436">
        <property type="entry name" value="Ta0600-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96820.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="94" mass="10578" checksum="5ED9B9FC47600966" modified="2013-06-26" version="1">MVFGTVAKDERSPEEILKGVAMMLDEIINDTTVPRNIRAAAEKAKNVVLNEKEEPIVRSATAIHILDEISNDPNMPLHTRTQIWSIVSELERVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSJ0</accession>
    <name>N6VSJ0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Na+/H+ antiporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_04068</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000026">
        <property type="protein sequence ID" value="ENN96131.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591533.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSJ0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00727"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.797"/>
    <dbReference type="OrthoDB" id="11709at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015297">
        <property type="term" value="F:antiporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1902600">
        <property type="term" value="P:proton transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.1330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006153">
        <property type="entry name" value="Cation/H_exchanger_TM"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32507">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32507:SF0">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER 2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00999">
        <property type="entry name" value="Na_H_Exchanger"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0050">Antiport</keyword>
    <keyword evidence="6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="35"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="60"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="94"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="217"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="245"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="275"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="307"/>
            <end position="332"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="352"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="389"/>
            <end position="412"/>
        </location>
    </feature>
    <feature type="domain" description="Cation/H+ exchanger transmembrane" evidence="9">
        <location>
            <begin position="15"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022449"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00999"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96131.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="426" mass="46549" checksum="8298957A0322DD5D" modified="2013-06-26" version="1">MELMSAIGFLGLSLVLGSIMAKISEKLNIPDIPLLLMLGLILGPILQIIPQGGALEIFEYVGPIGLVFILLGGAFTMRISLLKKVIKTVIRLDTITLMVTLIISGIFFNFILNLPITSPVGYLFGAITCATDPATLIPVFSKVKVDPEIAITLEAESIFNDPLGIVATSTILGMLGLAYVKNPILYFLSLSLGAIIAGVIFAKIYKYIIYRCEFHEYVSPLVLGGAMLLLYLGEHLIPKYINYGYSGYMAIAVMALYLGNELYKLMGDKHKNYEYIVNFSNDLSLLARIFIFVFLGACINMEMLIKYLIPGTIVALVSIFIARPLGVFIGLLGSNISFKKCLYFALEGPRGVVPAALAATVGVMILKHADKLPKYVITHISPKDFSGMIIIGTFLTILLSVILEASWAGILARKLIGEYKGKKYDH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSK5</accession>
    <name>N6VSK5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">2-oxoglutarate ferredoxin oxidoreductase subunit gamma</fullName>
            <ecNumber evidence="4">1.2.7.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_03803</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.3" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96151.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591351.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSK5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00392"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.745"/>
    <dbReference type="OrthoDB" id="18183at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.920.10">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052554">
        <property type="entry name" value="2-oxoglutarate_synth_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011894">
        <property type="entry name" value="PorC_KorC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019752">
        <property type="entry name" value="Pyrv/ketoisovalerate_OxRed_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002869">
        <property type="entry name" value="Pyrv_flavodox_OxRed_cen"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02175">
        <property type="entry name" value="PorC_KorC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006323">
        <property type="entry name" value="PRK08537.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42730">
        <property type="entry name" value="2-OXOGLUTARATE SYNTHASE SUBUNIT KORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42730:SF1">
        <property type="entry name" value="2-OXOGLUTARATE SYNTHASE SUBUNIT KORC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01558">
        <property type="entry name" value="POR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53323">
        <property type="entry name" value="Pyruvate-ferredoxin oxidoreductase, PFOR, domain III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="1 4" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate/ketoisovalerate oxidoreductase catalytic" evidence="3">
        <location>
            <begin position="11"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96151.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="181" mass="19824" checksum="BA412DCC3FEEDE62" modified="2013-06-26" version="1">MRREIRLSGFGGQGIILAGVILGKAAALYDNKEAVQTQSYGPEARGGYSKSEVVISDEPIDYPKVIKPDILVCMSQQAYDKYKDDIKENGTLIVDEDLVSLNELPKVKVNIYKIPFTRIASEKIGLPIVANIVMLGSLVRITNIVSKEAMEKAILNSVPKGTEEKNLLAFKLGYEVAGDVH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSN7</accession>
    <name>N6VSN7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rubredoxin-like non-heme iron protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03568</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96196.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591243.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSN7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01389"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.696"/>
    <dbReference type="OrthoDB" id="255599at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024934">
        <property type="entry name" value="Rubredoxin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048574">
        <property type="entry name" value="RUBY_RBDX"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21349">
        <property type="entry name" value="RUBY_RBDX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57802">
        <property type="entry name" value="Rubredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50903">
        <property type="entry name" value="RUBREDOXIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rubredoxin-like" evidence="1">
        <location>
            <begin position="1"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50903"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96196.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="61" mass="7096" checksum="3B43FC9A931004D0" modified="2013-06-26" version="1">MPWWKCSNCGYVFEADKPPERCPNCGEKCTFYDVTCYTPECGFTGYDPKLVARKPNEESRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSR7</accession>
    <name>N6VSR7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">site-specific DNA-methyltransferase (adenine-specific)</fullName>
            <ecNumber evidence="1">2.1.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_03443</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N(6)-methyl-2'-deoxyadenosine in DNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15197"/>
            <dbReference type="Rhea" id="RHEA-COMP:12418"/>
            <dbReference type="Rhea" id="RHEA-COMP:12419"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:90615"/>
            <dbReference type="ChEBI" id="CHEBI:90616"/>
            <dbReference type="EC" id="2.1.1.72"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.72" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000021">
        <property type="protein sequence ID" value="ENN96241.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591070.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSR7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00936"/>
    <dbReference type="REBASE" id="68613">
        <property type="entry name" value="M.MviT80ORF3443P"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.673"/>
    <dbReference type="OrthoDB" id="45790at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008170">
        <property type="term" value="F:N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009007">
        <property type="term" value="F:site-specific DNA-methyltransferase (adenine-specific) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022749">
        <property type="entry name" value="D12N6_MeTrfase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003356">
        <property type="entry name" value="DNA_methylase_A-5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038333">
        <property type="entry name" value="T1MK-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052916">
        <property type="entry name" value="Type-I_RE_MTase_Subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42998:SF1">
        <property type="entry name" value="TYPE I RESTRICTION ENZYME HINDI METHYLASE SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42998">
        <property type="entry name" value="TYPE I RESTRICTION ENZYME HINDVIIP M PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12161">
        <property type="entry name" value="HsdM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02384">
        <property type="entry name" value="N6_Mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00507">
        <property type="entry name" value="N12N6MTFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0680">Restriction system</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N6 adenine-specific DNA methyltransferase N-terminal" evidence="9">
        <location>
            <begin position="22"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="domain" description="DNA methylase adenine-specific" evidence="8">
        <location>
            <begin position="175"/>
            <end position="488"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="494"/>
            <end position="528"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF02384"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF12161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96241.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="536" mass="62626" checksum="E783933EA04DCC50" modified="2013-06-26" version="1">MTKIVKLDNFIETSYELPPDFENKLWKAADKLRKKVEVHEYKYVVLGLIFLRYLSYAFEERKNELKKLFSDKNSPYYIEDEEIRKEALEDKDYYLEAGVLYIPEKARWDYIHKNANQPNIAEIIENAIEILENEYPEELKDVIPKVYLKSNLDSLDLSYLINVFSEIDFGYNHKAKDIFGRIYEYFLGKFTEVEGKRGGKFYTPRCLTRLIVEILDVKEGTIFDPACGSGGFFVSAIEKLEREDLDKYKLSIYGQDADVMAWRLTKMNLVIRGVSGDIRVGDSYHDDKFFDMKFDYVVSNPPFNDSEWDSKRIKPDDPRLKVGEYRVPVPPESNANYMWILHFIYHLNQNGKAGFVMANGALSAGGVEGKIREAIIKADLVYGIVSCPAKLFYNVSLPVSLWFIRKEKPEHMKGKILFINAKDLYKQVSRRLNVMTDEHINKIVEKFRLFEKGELDKIDEVGFARVASIEDVAKNNYVLTPGRYVGVKLDIDDRESFEEKMRIYSEELERLLKEEEDLTKKVREVFKEIGFKIWGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSS3</accession>
    <name>N6VSS3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">MCP methylation inhibitor CheC</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_03273</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96251.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590950.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSS3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01042"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.641"/>
    <dbReference type="OrthoDB" id="182374at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17911">
        <property type="entry name" value="CheC_ClassIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1550.10">
        <property type="entry name" value="CheC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028976">
        <property type="entry name" value="CheC-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028051">
        <property type="entry name" value="CheX-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050992">
        <property type="entry name" value="CheZ_family_phosphatases"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43693">
        <property type="entry name" value="PROTEIN PHOSPHATASE CHEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43693:SF1">
        <property type="entry name" value="PROTEIN PHOSPHATASE CHEZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13690">
        <property type="entry name" value="CheX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103039">
        <property type="entry name" value="CheC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Chemotaxis phosphatase CheX-like" evidence="3">
        <location>
            <begin position="97"/>
            <end position="183"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF13690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96251.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="248" mass="27646" checksum="42C0F1B8F44FD0C9" modified="2013-06-26" version="1">MRFGNVEISPKSWDIIVKAIIESTLGDSKMSANALTTLDPRYFKKIEDIGKFASEKAMRFITDMTGYSAELKVFNIRLITPPEIISEYGDEIKIFTKINFSGEISGTGVLIFSEKGALEISKAMLLGMGMECDNDEMDDMKISAINEICNILISAYVDSFANFMDTTLTMTPPEFITGMGGEILNKIFKDNNIKDDDIIMAFKSTMHICEVGESFEVLIVLPNESVDKLFKAITEGKVKDKLSVKFES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSY6</accession>
    <name>N6VSY6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_03089</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96311.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSY6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00164"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.606"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040088">
        <property type="entry name" value="MJ0103-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787:SF3">
        <property type="entry name" value="ARYLSULFATASE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787">
        <property type="entry name" value="FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01110">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="8">
        <location>
            <begin position="103"/>
            <end position="339"/>
        </location>
    </feature>
    <feature type="domain" description="TRAM" evidence="7">
        <location>
            <begin position="367"/>
            <end position="429"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96311.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="429" mass="49736" checksum="58ED1B506F0EB8EA" modified="2013-06-26" version="1">MTLDLSKYRMITNIKSKGNRLILEINKIYEVSIDIPYEEVEIEESVIKINTHPKRAENLKMGILNLISYHIANNLRSKITKRRVIYINEPFPLIGHTAFGLIERGRNIIQVRGHCGCNLNCIFCSVDEGEFSKTRKNDYYVDIDYLIKEYKKLAEYKGIKKLEAHLDGQGEPSLYYPLVDLVQALSDINVEGIVSMQSNGTVLDYKLIDELEEAGLHRINLSINAIDEKMAKMLAGRKDYNINKILDIAEYIKNSKIHLLIAPILLPNINDTEFKRVIDFAVDLDLRVKQNIINPLTNKRDPILGCQLCRVYQFGRKPKKMKVWNFEKFYELLKKYEIEYKKRGLDVKLILRPEDFGIHRRKRLPYPFKVGEVIRTKAILDGRIKGEVLAAERGRVIQIINTNEDIIGKRVKVRILRNKDNIIVGEVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT17</accession>
    <name>N6VT17_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Hydrogenase maturation factor HypA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hypA</name>
        <name evidence="6" type="ORF">J422_02634</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the maturation of [NiFe] hydrogenases. Required for nickel insertion into the metal center of the hydrogenase.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the HypA/HybF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96341.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590561.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT17"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00079"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.516"/>
    <dbReference type="OrthoDB" id="36835at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2320.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00213">
        <property type="entry name" value="HypA_HybF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020538">
        <property type="entry name" value="Hydgase_Ni_incorp_HypA/HybF_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000688">
        <property type="entry name" value="HypA/HybF"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00100">
        <property type="entry name" value="hypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34535">
        <property type="entry name" value="HYDROGENASE MATURATION FACTOR HYPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34535:SF3">
        <property type="entry name" value="HYDROGENASE MATURATION FACTOR HYPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01155">
        <property type="entry name" value="HypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004761">
        <property type="entry name" value="Hydrgn_mat_HypA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01249">
        <property type="entry name" value="HYPA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5" id="KW-0533">Nickel</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="2"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010748"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00213"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96341.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="123" mass="13713" checksum="FC2162BAD2E74D97" modified="2013-06-26" version="1">MHELSLANSILDAILSAIESEEKKGRKVKKVKEIYLEIGELVFVNVEQLKFAFEVISEGTACEGAKVNIEILKSKCRCVNCGYEGEPKIVDDFIISCPKCGGFRLEIIGGKELNIKNVIIEYM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTC0</accession>
    <name>N6VTC0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">Iron-sulfur cluster carrier protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_02090</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 8">Binds and transfers iron-sulfur (Fe-S) clusters to target apoproteins. Can hydrolyze ATP.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the Mrp/NBP35 ATP-binding proteins family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96441.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590218.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTC0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01167"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.411"/>
    <dbReference type="OrthoDB" id="8297at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140663">
        <property type="term" value="F:ATP-dependent FeS chaperone activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02037">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001119">
        <property type="entry name" value="Iron-sulfur cluster carrier protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02040">
        <property type="entry name" value="Mrp_NBP35"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000808">
        <property type="entry name" value="Mrp-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019591">
        <property type="entry name" value="Mrp/NBP35_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033756">
        <property type="entry name" value="YlxH/NBP35"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264:SF19">
        <property type="entry name" value="CYTOSOLIC FE-S CLUSTER ASSEMBLY FACTOR NUBP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23264">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN NBP35 YEAST -RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10609">
        <property type="entry name" value="ParA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01215">
        <property type="entry name" value="MRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0408">Iron</keyword>
    <keyword evidence="5 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="1 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="35"/>
            <end position="42"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00074706"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02040"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96441.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="274" mass="29574" checksum="C91F05BFF513151C" modified="2013-06-26" version="1">MECDRKDAKKLLEQQNAKIRERMSKIKHKIAILSGKGGVGKSTVTANLAVGLSLLNKKVGVLDGDIHGPNIPKILGANGEPMVAGDAILPIEVSGIKTISISYLLPDEATPVIWRGPRVSGAIRQFLADVAWGELDYLLIDTPPGTGDVQLTIMQSIPNIDGAIIVTTPDEISLLDVKKSISMCKMLNIPIIGIIENMSGFVCPYCNKEIDIFGKGAGEKIAKELGLRFLGRIPLDAKAREAQEKGKPMVLIDCRAGEAFKEIVNKIVEIVENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTP0</accession>
    <name>N6VTP0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8">Glutamyl-tRNA(Gln) amidotransferase subunit D</fullName>
            <shortName evidence="5">Glu-ADT subunit D</shortName>
            <ecNumber evidence="5 8">6.3.5.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">gatD</name>
        <name evidence="12" type="ORF">J422_01945</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 8">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln). The GatDE system is specific for glutamate and does not act on aspartate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5 8">Heterodimer of GatD and GatE.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5 8">Belongs to the asparaginase 1 family. GatD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.-" evidence="5 8"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96536.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590153.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTP0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00237"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.383"/>
    <dbReference type="OrthoDB" id="371959at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004067">
        <property type="term" value="F:asparaginase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd08962">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1170:FF:000001">
        <property type="entry name" value="L-asparaginase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1170">
        <property type="entry name" value="L-asparaginase, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00586">
        <property type="entry name" value="GatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006033">
        <property type="entry name" value="AsnA_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036152">
        <property type="entry name" value="Asp/glu_Ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006034">
        <property type="entry name" value="Asparaginase/glutaminase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020827">
        <property type="entry name" value="Asparaginase/glutaminase_AS1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027475">
        <property type="entry name" value="Asparaginase/glutaminase_AS2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040919">
        <property type="entry name" value="Asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011878">
        <property type="entry name" value="GatD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040918">
        <property type="entry name" value="GatD_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037222">
        <property type="entry name" value="GatD_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027473">
        <property type="entry name" value="L-asparaginase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027474">
        <property type="entry name" value="L-asparaginase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037152">
        <property type="entry name" value="L-asparaginase_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00519">
        <property type="entry name" value="asnASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02153">
        <property type="entry name" value="gatD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003217">
        <property type="entry name" value="PRK04183.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707:SF28">
        <property type="entry name" value="60 KDA LYSOPHOSPHOLIPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11707">
        <property type="entry name" value="L-ASPARAGINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00710">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17763">
        <property type="entry name" value="Asparaginase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18195">
        <property type="entry name" value="GatD_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500175">
        <property type="entry name" value="Glu_ADT_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001220">
        <property type="entry name" value="L-ASNase_gatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00139">
        <property type="entry name" value="ASNGLNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00057">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00870">
        <property type="entry name" value="Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141300">
        <property type="entry name" value="GatD N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53774">
        <property type="entry name" value="Glutaminase/Asparaginase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00144">
        <property type="entry name" value="ASN_GLN_ASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00917">
        <property type="entry name" value="ASN_GLN_ASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51732">
        <property type="entry name" value="ASN_GLN_ASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 5 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 5 8" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GatD N-terminal" evidence="11">
        <location>
            <begin position="1"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="L-asparaginase N-terminal" evidence="9">
        <location>
            <begin position="75"/>
            <end position="256"/>
        </location>
    </feature>
    <feature type="domain" description="Asparaginase/glutaminase C-terminal" evidence="10">
        <location>
            <begin position="286"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 6">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" evidence="5 7">
        <location>
            <position position="158"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="159"/>
        </location>
    </feature>
    <feature type="active site" evidence="5">
        <location>
            <position position="235"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10099"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU10100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU004457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00710"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF17763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF18195"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96536.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="421" mass="46877" checksum="DF9D8A65C0F46F24" modified="2013-06-26" version="1">MEIGDVVRIETDKGVFEGLLLPSIDENVITLKLKNGYNIGILKENIKNVRVIKKGEKPKYELPPLNIEKKHKKTVSILSTGGTVASKVDYSTGAVHPSFTADDLIRAVPEIADIANIEGRAILNILSENMKPKYWIKIAEEIKKEAKRADGIVITHGTDTMNYTAAALSFMVNADFPIILTGAQRSSDRPSSDAYLNLLSSVIAAKENIKGVFVVMHETSDDKYCLLHRGVKVRKCHSSRRDAFLSINSLPIAKIDPFSKNIIYLQEVERSEGSKKVEINTKLEEKVALIKVYPGMDGEIIRYYVDKGYKGIVLEGTGLGHAPEYVFDDIKYAIDNDVVVIMTTQTINGRVNMNVYSNGRKLLNLGVIGCEDMLPEVAYVKLMYLLANYSLEDVKKLINKNMVGEINYRSDFKAYGGNYGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTQ0</accession>
    <name>N6VTQ0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphopantetheine adenylyltransferase</fullName>
            <ecNumber evidence="7">2.7.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Dephospho-CoA pyrophosphorylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Pantetheine-phosphate adenylyltransferase</fullName>
            <shortName evidence="7">PPAT</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">coaD</name>
        <name evidence="9" type="ORF">J422_02015</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Reversibly transfers an adenylyl group from ATP to 4'-phosphopantetheine, yielding dephospho-CoA (dPCoA) and pyrophosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>(R)-4'-phosphopantetheine + ATP + H(+) = 3'-dephospho-CoA + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:19801"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="2.7.7.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the eukaryotic CoaD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.3" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96546.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590179.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTQ0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00248"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.395"/>
    <dbReference type="OrthoDB" id="53228at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004595">
        <property type="term" value="F:pantetheine-phosphate adenylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00647">
        <property type="entry name" value="PPAT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050385">
        <property type="entry name" value="Archaeal_FAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004821">
        <property type="entry name" value="Cyt_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023540">
        <property type="entry name" value="PPAT_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00125">
        <property type="entry name" value="cyt_tran_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001985">
        <property type="entry name" value="PRK00777.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43793:SF1">
        <property type="entry name" value="FAD SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01467">
        <property type="entry name" value="CTP_transf_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6 7" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="3 7 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Cytidyltransferase-like" evidence="8">
        <location>
            <begin position="5"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01467"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96546.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="151" mass="17478" checksum="8B29450EC17BB802" modified="2013-06-26" version="1">MKKVVVGGTFDVLHKGHKELLKFASKLGKLIIGITSDDFAKKYKKHEINSLDIRLRNLKKFLDEIKADYEIKIINDPFGDAIYNDYDIIVVTEETLNNAKKINEIREKNGLKPLKIIVFDYVLAENGKPISTTRIRKGEIDTEGRRLWKQY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU87</accession>
    <name>N6VU87_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">MrpA C-terminal/MbhD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00551</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96761.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589792.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU87"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00062"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.106"/>
    <dbReference type="OrthoDB" id="65862at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019213">
        <property type="entry name" value="EhaD-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09881">
        <property type="entry name" value="EhaD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="20"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="27"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96761.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="10258" checksum="D2B3CF2017B01425" modified="2013-06-26" version="1">MIEYIASLCCVLGAIGVIFYKNPINKIILLSLIEIGVILFIVYYYYLDIALITSLTEPICTVILLIGYMKYLSMVKSKKKYGRELPILTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VUC6</accession>
    <name>N6VUC6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Putative signal transduction protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_00070</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589707.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VUC6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01153"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.14"/>
    <dbReference type="OrthoDB" id="43333at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1280.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016486">
        <property type="entry name" value="UCP006591_CBS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF29">
        <property type="entry name" value="OS02G0818000 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006591">
        <property type="entry name" value="UCP006591_CBS_MJ1004"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 2" id="KW-0129">CBS domain</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="178"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="7"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="67"/>
            <end position="127"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="209" mass="24262" checksum="58A65515E16A7308" modified="2013-06-26" version="1">MKVKQLMSKDFVKIYEDYPIEKVIKLLKEHKKFSAPVLDEEDRLLGWITTIDLLGVTEFKKSIKEIMLPKEEVIIAYEDEEAREVVLKFVKYKVVSIPVLSRDDRVVGIVRNCDIVKTLAKLYEIPVYNIFKKLHEHLDIDWNELMEAAAIVTKKMSGEDITPEDYEKRIKNTTFGKAIWACGGLENFFVGLIEIGMVALARRLAKKRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6VUE8</accession>
    <name>N6VUE8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Pyridoxal 5'-phosphate synthase subunit PdxS</fullName>
            <shortName evidence="7">PLP synthase subunit PdxS</shortName>
            <ecNumber evidence="7">4.3.3.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Pdx1</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">pdxS</name>
        <name evidence="11" type="ORF">J422_00190</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the formation of pyridoxal 5'-phosphate from ribose 5-phosphate (RBP), glyceraldehyde 3-phosphate (G3P) and ammonia. The ammonia is provided by the PdxT subunit. Can also use ribulose 5-phosphate and dihydroxyacetone phosphate as substrates, resulting from enzyme-catalyzed isomerization of RBP and G3P, respectively.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>aldehydo-D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + phosphate + 3 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:31507"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58273"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
            <dbReference type="EC" id="4.3.3.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Cofactor biosynthesis; pyridoxal 5'-phosphate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">In the presence of PdxT, forms a dodecamer of heterodimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 8">Belongs to the PdxS/SNZ family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.6" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96821.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589727.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VUE8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01130"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.38"/>
    <dbReference type="OrthoDB" id="6840at2157"/>
    <dbReference type="UniPathway" id="UPA00245"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036381">
        <property type="term" value="F:pyridoxal 5'-phosphate synthase (glutamine hydrolysing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042823">
        <property type="term" value="P:pyridoxal phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008615">
        <property type="term" value="P:pyridoxine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04727">
        <property type="entry name" value="pdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01824">
        <property type="entry name" value="PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001852">
        <property type="entry name" value="PdxS/SNZ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033755">
        <property type="entry name" value="PdxS/SNZ_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033983">
        <property type="entry name" value="Thiazole_synthase_ThiG"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003215">
        <property type="entry name" value="PRK04180.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00343">
        <property type="entry name" value="pyridoxal 5'-phosphate synthase lyase subunit PdxS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31829:SF0">
        <property type="entry name" value="PYRIDOXAL 5'-PHOSPHATE SYNTHASE SUBUNIT SNZ1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01680">
        <property type="entry name" value="SOR_SNZ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05690">
        <property type="entry name" value="ThiG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF029271">
        <property type="entry name" value="Pdx1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01235">
        <property type="entry name" value="PDXS_SNZ_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51129">
        <property type="entry name" value="PDXS_SNZ_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 11" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0704">Schiff base</keyword>
    <feature type="domain" description="PdxS/SNZ N-terminal" evidence="9">
        <location>
            <begin position="7"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="domain" description="Thiazole synthase ThiG" evidence="10">
        <location>
            <begin position="236"/>
            <end position="291"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with D-ribose 5-phosphate" evidence="7">
        <location>
            <position position="80"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="250"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="271"/>
            <end position="272"/>
        </location>
        <ligand>
            <name>D-ribose 5-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:78346"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01824"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF05690"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96821.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="330" mass="36394" checksum="A4A4D459DE8332DD" modified="2013-06-26" version="1">MKKGTELLKRGFAKMVKHGVVMDVTNVEQAQIAEEAGAVAVMALERVPADIRKEGGVARMSDPALIEEIMDAVSIPVMAKCRIGHTAEARVLEAIGVDMIDESEVLTQADPFFHIYKKKFTVPFVCGARNLGEAVRRIWEGAAMIRTKGEAGTGNVVEAVRHMRLMNEAIRQISEMDDESIYEVAKVFSMRYAELAMQVRESLGYPHEVLENEPIYEGFTLNEIIDGIYKILLEIKKIKRLPVVNFAAGGIATPADAALMMSLGCDGIFVGSGIFKSENPLEMARAIVEATYNYDKPEVVAEVSKNLGMPMKGIDVTKLKEEEKLQIRGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VWV9</accession>
    <name>N6VWV9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methyl-accepting chemotaxis sensory transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_07127</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000074">
        <property type="protein sequence ID" value="ENN95577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VWV9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01211"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="7"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="34"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="3">
        <location>
            <begin position="120"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="158"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="4">
        <location>
            <position position="209"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="209" mass="23900" checksum="ED84812697633094" modified="2013-06-26" version="1" fragment="single">MNIDSRVILSMPIVGYIVIVLIFSIFISKAISDIIFLNTVSIIFGIFCFIIIKKLLITKLLYIEKISNEISRGNVNIEIKFKKSNDILDNIIYNLYNIKEFIIKKDKIYENNMSEIENFLNEIYRVMKAISNGSLTERISKQKGNKLEKLRVVINNALDSLSRLIGDLIEDVKKLNSEIHRAEEEVNRIKETSEQIADAANQVAVAATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX15</accession>
    <name>N6VX15_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06678</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000057">
        <property type="protein sequence ID" value="ENN95652.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593906.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX15"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00715"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1300"/>
    <dbReference type="OrthoDB" id="64786at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95652.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="98" mass="11466" checksum="B8D992EA2FF0292E" modified="2013-06-26" version="1">MVKKKTGKLHVRIFFDGSYIEGEFDIDAVIYLKNSLYKYIWTGKKDAILVWNIDEKKFLVLNPEKICGIEVIGSLMFVDDIPDKKIDVSKFKEFVEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXA6</accession>
    <name>N6VXA6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">dITP/XTP pyrophosphatase</fullName>
            <ecNumber evidence="10">3.6.1.66</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Non-canonical purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Non-standard purine NTP pyrophosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoside-triphosphate diphosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoside-triphosphate pyrophosphatase</fullName>
            <shortName evidence="10">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_06110</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Pyrophosphatase that catalyzes the hydrolysis of nucleoside triphosphates to their monophosphate derivatives, with a high preference for the non-canonical purine nucleotides XTP (xanthosine triphosphate), dITP (deoxyinosine triphosphate) and ITP. Seems to function as a house-cleaning enzyme that removes non-canonical purine nucleotides from the nucleotide pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>ITP + H2O = IMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:29399"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>XTP + H2O = XMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28610"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>dITP + H2O = dIMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28342"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61194"/>
            <dbReference type="ChEBI" id="CHEBI:61382"/>
            <dbReference type="EC" id="3.6.1.66"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10 11">Belongs to the HAM1 NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.66" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXA6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00542"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1190"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035870">
        <property type="term" value="F:dITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036220">
        <property type="term" value="F:ITP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017111">
        <property type="term" value="F:ribonucleoside triphosphate phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036222">
        <property type="term" value="F:XTP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009146">
        <property type="term" value="P:purine nucleoside triphosphate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00515">
        <property type="entry name" value="HAM1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000001">
        <property type="entry name" value="dITP/XTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01405">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020922">
        <property type="entry name" value="dITP/XTP_pyrophosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002637">
        <property type="entry name" value="RdgB/HAM1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011396">
        <property type="entry name" value="PRK14821.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00042">
        <property type="entry name" value="RdgB/HAM1 family non-canonical purine NTP pyrophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067:SF9">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11067">
        <property type="entry name" value="INOSINE TRIPHOSPHATE PYROPHOSPHATASE/HAM1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01725">
        <property type="entry name" value="Ham1p_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 10" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="4 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="10">
        <location>
            <position position="61"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="6"/>
            <end position="11"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="136"/>
            <end position="139"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="159"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="164"/>
            <end position="165"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008023"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00051875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00052017"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01405"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="183" mass="21038" checksum="37E841C24E04862D" modified="2013-06-26" version="1">MLYFATGNINKVKEAEIILKDIKIQQINIKYPEIQGSLEEVAEFGAKYVYNIIKKPVIVEDSGFFVEALNGFPGPYSRYVQETIGNEGILKLLEDKDNRNAYFKCVIGYCDENGVKLFSGIIRGKVSEEIRGEHGFAYDSIFIPEGESRTFAEMSREEKSRISHRKLAFDKLREFLKRGKYEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXH3</accession>
    <name>N6VXH3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Flagellin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05833</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95832.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592838.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXH3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01239"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1137"/>
    <dbReference type="OrthoDB" id="183655at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42200">
        <property type="entry name" value="ARCHAEAL FLAGELLA-RELATED PROTEIN F-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42200:SF1">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN G-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Flagellin_arch-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0966">Cell projection</keyword>
    <keyword evidence="2" id="KW-0969">Cilium</keyword>
    <keyword evidence="2" id="KW-0282">Flagellum</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="26"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95832.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="151" mass="16593" checksum="23848D9B9B476233" modified="2013-06-26" version="1">MASSGLSEIIMFVAVLLIAAFVVGVLTTSTYKISLNIGKKTDSFSEKLAQDFEIINDPAKIPRDPNLGIITLYIKNTGKKPIIFTPDSFTVIIDGNIVKIYNISQLSNPGSEELYPGDVGELNVSYNESRYHRIKVVLYSGVSRVIEGYIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXX0</accession>
    <name>N6VXX0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95982.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592137.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXX0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00479"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.990"/>
    <dbReference type="OrthoDB" id="371772at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017271">
        <property type="entry name" value="Tscrpt_reg_HTH_MJ1545_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037724">
        <property type="entry name" value="TF_HTH_MJ1545_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="22"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95982.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="231" mass="26077" checksum="666B7FCAF8AA8A7A" modified="2013-06-26" version="1">MEKVAIYIIGDIVLSENTGAALKKWRNLFNIQQIELANYLNISPSVISDYETGRRKNPGVNVIRKYVLALIEIDKRKGGYTIKALKRLLEKSPSMKAILSIKEYNNPIKIKDFAKILEGEIVVGSNLDAVIYGHTVVDSIKAIMEMTGEEFYHLYGWTTERALIFTNVSTGRSPMVAIRVSLMKPRVIVFQGLDKNKIDKLAIKLAEIDNIPIITTKLDIDELIKRLNEIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYF0</accession>
    <name>N6VYF0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03773</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96147.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591344.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYF0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00387"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.738"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12797">
        <property type="entry name" value="Fer4_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="34"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96147.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="91" mass="10754" checksum="A7DF1CC66C781B36" modified="2013-06-26" version="1">MEDIPVIGKDKLGRVIRDWSVKPWFGIDRKDIKWYPEINYEKCIGCGLCFLTCANRVVFDWDEEKKRPIVARPYNCVVACTPVKPYVQWML</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYF9</accession>
    <name>N6VYF9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proteasome subunit beta</fullName>
            <ecNumber evidence="9">3.4.25.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">20S proteasome beta subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">Proteasome core protein PsmB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">psmB</name>
        <name evidence="11" type="ORF">J422_03833</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>Cleavage of peptide bonds with very broad specificity.</text>
            <dbReference type="EC" id="3.4.25.1"/>
        </reaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="9">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="9">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the peptidase T1B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.25.1" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96157.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591362.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYF9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00398"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.751"/>
    <dbReference type="OrthoDB" id="6330at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019774">
        <property type="term" value="C:proteasome core complex, beta-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd03764">
        <property type="entry name" value="proteasome_beta_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000049">
        <property type="entry name" value="Proteasome subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02113_A">
        <property type="entry name" value="Proteasome_B_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019983">
        <property type="entry name" value="Pept_T1A_Psome_bsu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000243">
        <property type="entry name" value="Pept_T1A_subB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016050">
        <property type="entry name" value="Proteasome_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023333">
        <property type="entry name" value="Proteasome_suB-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03634">
        <property type="entry name" value="arc_protsome_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194:SF0">
        <property type="entry name" value="ATP-DEPENDENT PROTEASE SUBUNIT HSLV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32194">
        <property type="entry name" value="METALLOPROTEASE TLDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00141">
        <property type="entry name" value="PROTEASOME"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00854">
        <property type="entry name" value="PROTEASOME_BETA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51476">
        <property type="entry name" value="PROTEASOME_BETA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 9" id="KW-0645">Protease</keyword>
    <keyword evidence="7 9 11" id="KW-0647">Proteasome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0888">Threonine protease</keyword>
    <keyword evidence="8 9" id="KW-0865">Zymogen</keyword>
    <feature type="propeptide" id="PRO_5005075181" description="Removed in mature form; by autocatalysis" evidence="9">
        <location>
            <begin position="1"/>
            <end position="6"/>
        </location>
    </feature>
    <feature type="chain" id="PRO_5023399927" description="Proteasome subunit beta" evidence="9">
        <location>
            <begin position="7"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="9 10">
        <location>
            <position position="7"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022698"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023145"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02113"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRSR" id="PIRSR600243-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96157.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="212" mass="23099" checksum="65651259E625118A" modified="2013-06-26" version="1">MEIMKGTTTVGLICDDAVILATDKRASMGNLVADKEARKLYKIDDYIALTVAGSVGDAQALVRLLTAETRLYKMRTGRNISPLACATLLANILHSSRYFPFLTQLIIGGYDLNEGPKIFTLDPLGGMSEEKTFTATGSGSPVAYGVLEAEYDRNMKVEDGIKLAIKALKSAMERDTFSGNGINLAIVSKDGVKILEDEEINCIIKKRRSKKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYS6</accession>
    <name>N6VYS6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Deoxyribonuclease/rho motif-related TRAM</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03226</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000019">
        <property type="protein sequence ID" value="ENN96282.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590898.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYS6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00322"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.633"/>
    <dbReference type="OrthoDB" id="28569at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002792">
        <property type="entry name" value="TRAM_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01938">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50926">
        <property type="entry name" value="TRAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TRAM" evidence="1">
        <location>
            <begin position="13"/>
            <end position="71"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96282.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="71" mass="7944" checksum="7C63D12CC318656A" modified="2013-06-26" version="1">MFKNNKDKGKRVPVKEGETYKVKIEDMGKGGDGIARVEGFVVFVPETHKGDEVNIKITSVKSRFAFGEKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYZ8</accession>
    <name>N6VYZ8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02689</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96352.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590584.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYZ8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00090"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.527"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96352.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="136" mass="16066" checksum="9959723453A9D7FA" modified="2013-06-26" version="1">MLKYIIFFQNFVKKGSLKILLTNIFLLEINKDEIILNLENPKTFKEIFIIVERIIKTIKNDKKEDSFLKKIENFKNLLDSLKKLSNILINEKKTFILRYKNEDVIVIGYKAKGGLLLKDIKINKLLLLKLLKELNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ26</accession>
    <name>N6VZ26_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02869</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96382.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590644.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ26"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00123"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.563"/>
    <dbReference type="OrthoDB" id="63159at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96382.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="95" mass="10954" checksum="D757FE902C06016C" modified="2013-06-26" version="1">MKCPYCGGDCIDKDMVNLYLKSVEMFFKFQSDNELVKKYPPAGEIGECKKTFNRIYLCPYCKEPFKAYYNNGKAKIVCPNCKKTLLLPITNRSVC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ32</accession>
    <name>N6VZ32_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Cell division protein CDC48</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_02904</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the AAA ATPase family. CDC48 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96387.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590655.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ32"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00129"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.570"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd19519">
        <property type="entry name" value="RecA-like_CDC48_r1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd19529">
        <property type="entry name" value="RecA-like_VCP_r2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.40.20:FF:000007">
        <property type="entry name" value="AAA family ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000057">
        <property type="entry name" value="AAA family ATPase, CDC48 subfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000178">
        <property type="entry name" value="CDC48/VCP homolog, AAA superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000018">
        <property type="entry name" value="Cell division control 48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000012">
        <property type="entry name" value="Transitional endoplasmic reticulum ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.330.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005938">
        <property type="entry name" value="AAA_ATPase_CDC48"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050168">
        <property type="entry name" value="AAA_ATPase_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041569">
        <property type="entry name" value="AAA_lid_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009010">
        <property type="entry name" value="Asp_de-COase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004201">
        <property type="entry name" value="Cdc48_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029067">
        <property type="entry name" value="CDC48_domain_2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003338">
        <property type="entry name" value="CDC4_N-term_subdom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015415">
        <property type="entry name" value="Spast_Vps4_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01243">
        <property type="entry name" value="CDC48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077">
        <property type="entry name" value="AAA-FAMILY ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23077:SF171">
        <property type="entry name" value="NUCLEAR VALOSIN-CONTAINING PROTEIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17862">
        <property type="entry name" value="AAA_lid_3"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02933">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02359">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09336">
        <property type="entry name" value="Vps4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01072">
        <property type="entry name" value="CDC48_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01073">
        <property type="entry name" value="CDC48_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50692">
        <property type="entry name" value="ADC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54585">
        <property type="entry name" value="Cdc48 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="9" id="KW-0132">Cell division</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CDC48 N-terminal subdomain" evidence="8">
        <location>
            <begin position="2"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="domain" description="CDC48" evidence="7">
        <location>
            <begin position="102"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="210"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="domain" description="AAA+ ATPase" evidence="6">
        <location>
            <begin position="483"/>
            <end position="620"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="744"/>
            <end position="805"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01072"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96387.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="902" mass="101231" checksum="FF13481A3BFDE565" modified="2013-06-26" version="1">MELKVAEAYQGDVGRGIARIDPYTMEELGLKPGDVIEIEGPKGKAYAIVYRGFLEDAGKKVIRIDGYLRQNAGVAIGDKVKVKKVEIKEAKKIVLAPTQPIRFGPGFEDFIKRKILGQVLNKGSKVTIGVLGTALSFVVVSTIPKGPVRITEFTDVELKEEPVKEIAESKIPDVTYEDIGGLKEEIRKVREMIELPMRHPELFEKLGIEPPKGVLLIGPPGTGKTLLAKAVANEAGANFYVINGPEIMSKYVGETEENLRKIFEEAEENAPSIIFIDEIDAIAPKRDEVTGEVERRLVAQLLTLMDGLKGRGQVIVIGATNRPDAIDPALRRPGRFDREIYIGVPDREGRKEILQIHTRNMPLAEDVDLDYLADVTHGFVGADLAALCKEAAMRALRRVLPDIDLEAEEIPKEILDNLKVTMDDFKEALKDIEPSAMREVLVDIPNVKWEDIGGLEEVKQELREAVEWPIKAKEVFDKIGVRPPKGVLLFGPPGTGKTLLAKAVANEAGANFISVKGPEIFSKWVGESEKAIREIFKKARQNAPCIIFFDEIDAIAPKRGRDISTGVTDKVVNQLLTELDGMEEPKDVVVIAATNRPDIIDPALLRPGRLDRVILVPPPDEKARFEIFKIHTKNLNLAEDVDLEELAKKTDGYTGADIEAICREAAMLAVREKLGEPWEIEKALKDLILYMQSISGIFRITAVELNSVIKSTKEKEKVDKEEFRELKASVTKIVSVLKPLKEKLDKVENEIEGFIKIIENSELKPSEKDEAKKLSNYLRDMINKLKETIDNIYKLENTLLELNKEVSSEKVDEIIKETQNILQRFSTTLEEFKNILKDIESIRLRLTSKDVKVRKEHFLKAIEKIKPSVSKEDMKLYERLAEEYGRSTTNKKEKKEESREVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZA5</accession>
    <name>N6VZA5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Tetrahydromethanopterin S-methyltransferase subunit H</fullName>
            <ecNumber evidence="6">2.1.1.86</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_02145</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the MtrH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.86" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96452.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZA5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01177"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.421"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023467">
        <property type="entry name" value="MeTrfase_MtrH/MtxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028342">
        <property type="entry name" value="MtrH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01114">
        <property type="entry name" value="mtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02007">
        <property type="entry name" value="MtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF500206">
        <property type="entry name" value="MtrH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004960">
        <property type="entry name" value="MtrH_MtxH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6" id="KW-0808">Transferase</keyword>
    <keyword evidence="5" id="KW-1278">Translocase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96452.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="297" mass="32422" checksum="4239D923B4CAF695" modified="2013-06-26" version="1">MFKFDREQMVVEIAGRKIGGQPGEYPTALAGTIFYARHKIVEDERKGIFDKAAAEELINKQAEMEEITGNPALVQVFGATPEAIVKYVDFVVEIWDGPILLDSTDKKARMEAAKRATEAGYSKQCIYNSINVSIDDEELKNLIESDLEASIILCFDPMDSSVEGKLNVLLNGGKAADKGMLELAEKAGIKKPLIDVAVTPLGNGAGNAIRASFAVKAKLGLPVGSGIHNVPSAWDWLRQFRKQLREAGKTQLAKDVHHVCDIGANLLQVMAAGDFVLYGPIDNAQLASQLLLWQIWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZE0</accession>
    <name>N6VZE0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_02305</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic/archaeal PrmC-related family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96482.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590298.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZE0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01208"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.453"/>
    <dbReference type="OrthoDB" id="27149at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035657">
        <property type="term" value="C:eRF1 methyltransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008276">
        <property type="term" value="F:protein methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052190">
        <property type="entry name" value="Euk-Arch_PrmC-MTase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004557">
        <property type="entry name" value="PrmC-related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00537">
        <property type="entry name" value="hemK_rel_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011529">
        <property type="entry name" value="PRK14968.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45875:SF1">
        <property type="entry name" value="METHYLTRANSFERASE N6AMT1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="5">
        <location>
            <begin position="16"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96482.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="190" mass="21425" checksum="D3A1272BFE07449F" modified="2013-06-26" version="1">MKLKFHKYVYEPAEDSYLLLNNLVDVEGKEVLEIGTGCGLIAIACYKKGAKRVVATDINPYAVKLAKYNAKLNNAKIEIIESDLFENIKGKFDVIIFNPPYLPTEEDEKLDGYINYAFDGGKDGRAVLDRFLKEAPKYLKDGGVIQLVQSSLNDKDKTLSILKNLGFKVEIKAKLKLPFEELYIINAKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZE7</accession>
    <name>N6VZE7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 12">Biotin synthase</fullName>
            <ecNumber evidence="3 12">2.8.1.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">bioB</name>
        <name evidence="15" type="ORF">J422_01660</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>(4R,5S)-dethiobiotin + (sulfur carrier)-SH + 2 reduced [2Fe-2S]-[ferredoxin] + 2 S-adenosyl-L-methionine = (sulfur carrier)-H + biotin + 2 5'-deoxyadenosine + 2 L-methionine + 2 oxidized [2Fe-2S]-[ferredoxin]</text>
            <dbReference type="Rhea" id="RHEA:22060"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="Rhea" id="RHEA-COMP:14737"/>
            <dbReference type="Rhea" id="RHEA-COMP:14739"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:29917"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57586"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64428"/>
            <dbReference type="ChEBI" id="CHEBI:149473"/>
            <dbReference type="EC" id="2.8.1.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </cofactor>
        <text evidence="12">Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="13">
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49601"/>
        </cofactor>
        <text evidence="13">Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12 13">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="12 13">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 12">Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="12">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the radical SAM superfamily. Biotin synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.8.1.6" evidence="3 12"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96487.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590092.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZE7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00184"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.326"/>
    <dbReference type="OrthoDB" id="9264at2157"/>
    <dbReference type="UniPathway" id="UPA00078">
        <property type="reaction ID" value="UER00162"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004076">
        <property type="term" value="F:biotin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009102">
        <property type="term" value="P:biotin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000605">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01694">
        <property type="entry name" value="BioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010722">
        <property type="entry name" value="BATS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002684">
        <property type="entry name" value="Biotin_synth/BioAB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024177">
        <property type="entry name" value="Biotin_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00433">
        <property type="entry name" value="bioB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976">
        <property type="entry name" value="BIOTIN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22976:SF2">
        <property type="entry name" value="BIOTIN SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06968">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001619">
        <property type="entry name" value="Biotin_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01060">
        <property type="entry name" value="BATS_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01278">
        <property type="entry name" value="biotin_synthase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00876">
        <property type="entry name" value="BATS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 12 13" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="4 12 13" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9 12" id="KW-0093">Biotin biosynthesis</keyword>
    <keyword evidence="10 12 13" id="KW-0408">Iron</keyword>
    <keyword evidence="11 12 13" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="8 12 13" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 12 13" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5 12" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Radical SAM core" evidence="14">
        <location>
            <begin position="26"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="119"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12 13">
        <location>
            <position position="254"/>
        </location>
        <ligand>
            <name>[2Fe-2S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:190135"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010765"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001619-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN96487.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="302" mass="34313" checksum="47946A2DB93423D0" modified="2013-06-26" version="1">MNIEKAIELYNTLSTIDLLYLAYKKKTKKDIDLCAIINAKSGKCKENCIFCSQSIYHNTSIPIYKLKSKDEILEYALKYDGIVNRFSIVVSGKKVNDEEFNKIVETIELLKDETSLKICCSLGLIDKEKLRILKDLDVRIHNNLETNKDYFKNICTTHSYMDKVRVIKDAKSLNLEVCSGGIIGLGESYLDRIKLAFELKDLGVDSVPINILHPIKGTKAYDLLKRGVIKPLSVNEILKTIAIFKLILKDCEIRLAGGRLFLGDYQSYALLALDGLMVGDYLTTKGRNLKDDLKMIKDWELL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZG0</accession>
    <name>N6VZG0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Pyruvate ferredoxin oxidoreductase subunit beta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_01720</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="1">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96497.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590102.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZG0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00195"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.338"/>
    <dbReference type="OrthoDB" id="296931at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd03376">
        <property type="entry name" value="TPP_PFOR_porB_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051479">
        <property type="entry name" value="PorB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011766">
        <property type="entry name" value="TPP_enzyme_TPP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008819">
        <property type="entry name" value="PRK11865.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42897:SF2">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02775">
        <property type="entry name" value="TPP_enzyme_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme TPP-binding" evidence="3">
        <location>
            <begin position="42"/>
            <end position="211"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02775"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96497.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="295" mass="32738" checksum="EF3750BB087FB93C" modified="2013-06-26" version="1">MQYPRIELFAPGHRGCAGCGAAIIVRQILKVAGKDTIVTTPTGCLEVFSTPYPETSWKVPWIHVAFECAGAVASGIESAIKVLKRKRGKFKDRKINVIAIGGDGGTADIGFQSLSGAMERGHNILYIMYDNEAYMNTGIQRSSATPLYASTTTTPAGKISKGEDRPKKDMAMIMAAHGVPYVATACVSYPDDLMRKVKKALSIEGPKFIHVLQPCTTGWGFPPEKTIEIGRLAVETGVFPLYEIEYGEFRLTYRPAKRKPVKEYIRAQKRYRHLTDEDIERLQKYIDEKCKLLGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZU6</accession>
    <name>N6VZU6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable L-tyrosine/L-aspartate decarboxylase</fullName>
            <shortName evidence="6">TDC/ADC</shortName>
            <ecNumber evidence="6">4.1.1.11</ecNumber>
            <ecNumber evidence="6">4.1.1.25</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">mfnA</name>
        <name evidence="8" type="ORF">J422_01338</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the decarboxylation of L-tyrosine to produce tyramine for methanofuran biosynthesis. Can also catalyze the decarboxylation of L-aspartate to produce beta-alanine for coenzyme A (CoA) biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-aspartate + H(+) = beta-alanine + CO2</text>
            <dbReference type="Rhea" id="RHEA:19497"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="EC" id="4.1.1.11"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>L-tyrosine + H(+) = tyramine + CO2</text>
            <dbReference type="Rhea" id="RHEA:14345"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:58315"/>
            <dbReference type="ChEBI" id="CHEBI:327995"/>
            <dbReference type="EC" id="4.1.1.25"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 6 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; methanofuran biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the group II decarboxylase family. MfnA subfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.11" evidence="6"/>
    <dbReference type="EC" id="4.1.1.25" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589931.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZU6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00021"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.262"/>
    <dbReference type="OrthoDB" id="56891at2157"/>
    <dbReference type="UniPathway" id="UPA00080"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004068">
        <property type="term" value="F:aspartate 1-decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004837">
        <property type="term" value="F:tyrosine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001120">
        <property type="term" value="P:methanofuran biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01610">
        <property type="entry name" value="MfnA_decarbox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050477">
        <property type="entry name" value="GrpII_AminoAcid_Decarb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020931">
        <property type="entry name" value="MfnA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002129">
        <property type="entry name" value="PyrdxlP-dep_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03812">
        <property type="entry name" value="tyr_de_CO2_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42735:SF6">
        <property type="entry name" value="SPHINGOSINE-1-PHOSPHATE LYASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00282">
        <property type="entry name" value="Pyridoxal_deC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4 6 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="6 7">
        <location>
            <position position="223"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038302"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR602129-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="370" mass="41941" checksum="76A29A8211FE962A" modified="2013-06-26" version="1">MVKSEILDELKKYREMDLKYEDGKIFGSMCSNVFPITKKIVEMFLETNLGDPGLFKGTKLLEEKAIKLIGELLNNKNPYGHIVSGGTEANLMVLRCIKNMCKERNEKPIIVLPVTAHFSFEKGRDMMDLNYIYAPIKKDLTVDEKFVKDAVEDYNASAIVGIAGTTEFGTVDNIEELSKIAKDNECYIHVDAAFGGFVIPFLKKKYKFDFSLGVDSITIDPHKMALCPIPAGGIVFKNIGYKKYLITEAPYLTETAQATILGTRVGYGAACTYAILKYYKDKIREIVNVCMENSYYLYKKLKENGFDVIKPTLNIVNILDDDYINTYNKLKEKGYYLSICKNLKALRIVVMPHVKKEHIDALIEVLKEIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZV3</accession>
    <name>N6VZV3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FeMo cofactor biosynthesis protein NifB</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Nitrogenase cofactor maturase NifB</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="13">Radical SAM assemblase NifB</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">J422_01388</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Involved in the biosynthesis of the iron-molybdenum cofactor (FeMo-co or M-cluster) found in the dinitrogenase enzyme of the nitrogenase complex in nitrogen-fixing microorganisms. NifB catalyzes the crucial step of radical SAM-dependent carbide insertion that occurs concomitant with the insertion of a 9th sulfur and the rearrangement/coupling of two [4Fe-4S] clusters into a [8Fe-9S-C] cluster, the precursor to the M-cluster.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; Fe-Mo cofactor biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the radical SAM superfamily. NifB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96612.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZV3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00011"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.272"/>
    <dbReference type="OrthoDB" id="53113at2157"/>
    <dbReference type="UniPathway" id="UPA00782"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787:SF13">
        <property type="entry name" value="FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787">
        <property type="entry name" value="FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0408">Iron</keyword>
    <keyword evidence="10" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="12" id="KW-0456">Lyase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-0535">Nitrogen fixation</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="15">
        <location>
            <begin position="19"/>
            <end position="261"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003522"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00006804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021702"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN96612.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="295" mass="34135" checksum="F926688BA91AE9A7" modified="2013-06-26" version="1">MSKFAHITKIHPCFNEKIHDKVGRIHLPVALKCNIACKFCRRSIGKEACEQRPGVARAILKPEDVESYLKRMLDKYPNIKVVGIAGPGDSLFNKETFETLKIIDEKFPNLIKCISTNGLLLPKYYKTLADLNVKTVTVTVNAINPEILKEIVERVYYNKKIYKGIEGAEILIKNQLEGIRKAYDEDLIIKINTVLIPEINKEHVVEIAKELKDYAYIQNIIPLIPLYKMKNYRPPTCEELKKVREEAEKYLPQFRACAQCRADAVGLIKEKEELKNYFKEQNKELDVFEIKHFSH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="64" xmlns="https://uniprot.org/uniprot">
    <accession>N6W012</accession>
    <name>N6W012_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 13 15">Glyceraldehyde-3-phosphate dehydrogenase</fullName>
            <shortName evidence="13">GAPDH</shortName>
            <ecNumber evidence="4 13 15">1.2.1.59</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10 13">NAD(P)-dependent glyceraldehyde-3-phosphate dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="primary">gap</name>
        <name evidence="17" type="ORF">J422_00911</name>
    </gene>
    <organism evidence="17 18">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="17 18" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="17 18">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 13 15">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = (2R)-3-phospho-glyceroyl phosphate + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10300"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 13 15">
            <text>D-glyceraldehyde 3-phosphate + phosphate + NADP(+) = (2R)-3-phospho-glyceroyl phosphate + NADPH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:10296"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57604"/>
            <dbReference type="ChEBI" id="CHEBI:57783"/>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="1.2.1.59"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 13 15">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 13 15">Homotetramer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="13 15">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 15">Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="17">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.1.59" evidence="4 13 15"/>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589852.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W012"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00592"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.179"/>
    <dbReference type="OrthoDB" id="295712at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00184"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008839">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate reductase"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004365">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047100">
        <property type="term" value="F:glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050661">
        <property type="term" value="F:NADP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18127">
        <property type="entry name" value="GAPDH_II_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd02278">
        <property type="entry name" value="GAPDH_II_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.10">
        <property type="entry name" value="Dihydrodipicolinate Reductase, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00559">
        <property type="entry name" value="G3P_dehdrog_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000846">
        <property type="entry name" value="DapB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020831">
        <property type="entry name" value="GlycerAld/Erythrose_P_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020830">
        <property type="entry name" value="GlycerAld_3-P_DH_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020829">
        <property type="entry name" value="GlycerAld_3-P_DH_cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020828">
        <property type="entry name" value="GlycerAld_3-P_DH_NAD(P)-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006436">
        <property type="entry name" value="Glyceraldehyde-3-P_DH_2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01546">
        <property type="entry name" value="GAPDH-II_archae"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003251">
        <property type="entry name" value="PRK04207.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01113">
        <property type="entry name" value="DapB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02800">
        <property type="entry name" value="Gp_dh_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000149">
        <property type="entry name" value="GAP_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00846">
        <property type="entry name" value="Gp_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55347">
        <property type="entry name" value="Glyceraldehyde-3-phosphate dehydrogenase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00071">
        <property type="entry name" value="GAPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13 15" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 13 15" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="8 13 15" id="KW-0520">NAD</keyword>
    <keyword evidence="6 13 15" id="KW-0521">NADP</keyword>
    <keyword evidence="7 13 15" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="18" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glyceraldehyde 3-phosphate dehydrogenase NAD(P) binding" evidence="16">
        <location>
            <begin position="2"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="13 14">
        <location>
            <position position="143"/>
        </location>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="11"/>
            <end position="12"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="113"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="142"/>
            <end position="144"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <begin position="197"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>D-glyceraldehyde 3-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="306"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00021022"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00030647"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048067"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00559"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000149-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU003388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="SMART" id="SM00846"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="EMBL" id="ENN96687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="342" mass="38212" checksum="A6760EA5BE5E0BEE" modified="2013-06-26" version="1">MVKVLINGYGSIGKRVADAVSKQKDMEVLGVTKTKPDFEARLAVEKGYKLYCAIPDKERLKAFEDAGIDVEGTILDVIEEADIVVDAAPKKIGKQNLESIYKPHKVKAILQGGEKANDVEDNFNALWSYERCFGKDYVRVVSCNTTGLCRILYAINSVVEINKARIVLVRRAVDPNDDKNGPVNAIKPNPVVVPSHHGPDVVSVIPEFEGKIITSAVIVPTTLMHMHTLMVEVNNNISREKLLEAIKETPRIKLFKAEEGFESTAKIIEYARDIGRLRYDLPELAVWEESVNVLENEIFLMQAVHQESIVIPENIDCIRAMLELESDKFKSIDKTNKALGIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6W074</accession>
    <name>N6W074_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">(NiFe)-hydrogenase-3-type complex Eha, membrane protein EhaE</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00556</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96762.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589793.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W074"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00063"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.107"/>
    <dbReference type="OrthoDB" id="81881at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011317">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09880">
        <property type="entry name" value="EhaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036535">
        <property type="entry name" value="EhaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="54"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96762.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="81" mass="9200" checksum="31D3C6AFADB6C52F" modified="2013-06-26" version="1">MIILYISYFLLIFGTLGALVGYDTKDPLIRFLNLEVVNMGVCLAFLAYDMALALMTFIAVNTILTIIFVRSIILYKKLEDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6W0A6</accession>
    <name>N6W0A6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Iron dependent repressor</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_00085</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the DtxR/MntR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96802.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589708.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W0A6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01151"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.17"/>
    <dbReference type="OrthoDB" id="24735at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046983">
        <property type="term" value="F:protein dimerization activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.60.10">
        <property type="entry name" value="Iron dependent repressor, metal binding and dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050536">
        <property type="entry name" value="DtxR_MntR_Metal-Reg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001367">
        <property type="entry name" value="Fe_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036421">
        <property type="entry name" value="Fe_dep_repressor_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022687">
        <property type="entry name" value="HTH_DTXR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000835">
        <property type="entry name" value="HTH_MarR-typ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022689">
        <property type="entry name" value="Iron_dep_repressor"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238">
        <property type="entry name" value="IRON (METAL) DEPENDENT REPRESSOR, DTXR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33238:SF7">
        <property type="entry name" value="IRON-DEPENDENT TRANSCRIPTIONAL REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02742">
        <property type="entry name" value="Fe_dep_repr_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01325">
        <property type="entry name" value="Fe_dep_repress"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00529">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00347">
        <property type="entry name" value="HTH_MARR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47979">
        <property type="entry name" value="Iron-dependent repressor protein, dimerization domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50944">
        <property type="entry name" value="HTH_DTXR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0804">Transcription</keyword>
    <keyword evidence="2" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH dtxR-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007871"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50944"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96802.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="126" mass="14734" checksum="192610A6F77FE968" modified="2013-06-26" version="1">MSRSIEDYLEKIYLFVKENKRPIKTTELAKMLNINPSAITNMAKRLKELGYVKYEPYVGISLTKEGEEIAKKIIEKHQTIKTFLIICLGLNEEEADEEACKLEHVVSDKVLDRLKMFIEDYKKVKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTI1</accession>
    <name>N6UTI1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Putative HTH-type transcriptional regulatory protein J422_06877</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_06877</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95624.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594256.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTI1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01072"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1334"/>
    <dbReference type="OrthoDB" id="31424at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00584">
        <property type="entry name" value="HTH_type_cro_C1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020886">
        <property type="entry name" value="MTH_967-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR059051">
        <property type="entry name" value="MTH_967_PDDEXK"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003162">
        <property type="entry name" value="PRK04140.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26553">
        <property type="entry name" value="PDDEXK_19"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="5">
        <location>
            <begin position="128"/>
            <end position="186"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00584"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95624.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="313" mass="35555" checksum="2DB6B0A0FC80C539" modified="2013-06-26" version="1">MREMLIAECIELLKHHKFIVSKPLGRSCFDIIASKDDVKLILKILKNIDSLSKEQSIELKKISKILAGTPLIIGLRTRNAPMEYGIVYDRYNIKAVTFETFKDFLEGNPPIVYVQRGGLFVKIDGQVLREVREALGISAGELADAVGVSRKTIYKYEAGLASPSLDIAIKIEEYLDVPLVKAIDLFEPINDENIEDKVEKLEDYKREAINFLSELGFRSIVVNKAPFDAVAEKNVEEMQNILLTNIEEKIDEEVLKKAIIVKEISKMLNSYSLLILEKKEKELKNIPTVSMEELKKMDDALELIEHIKKMLSS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTL4</accession>
    <name>N6UTL4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">TPR repeat-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_06553</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000055">
        <property type="protein sequence ID" value="ENN95669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593711.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTL4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00608"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1275"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013105">
        <property type="entry name" value="TPR_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558">
        <property type="entry name" value="CELL DIVISION CYCLE 16,23,27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12558:SF13">
        <property type="entry name" value="CELL DIVISION CYCLE PROTEIN 27 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07719">
        <property type="entry name" value="TPR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13174">
        <property type="entry name" value="TPR_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="9"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50293">
        <property type="entry name" value="TPR_REGION"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="52"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="120"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="154"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="206"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="381"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="6">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="572" mass="68163" checksum="970D6B2882276564" modified="2013-06-26" version="1">MIFKIFKKLFFLFKKPKFNKLIKKAEHHLDIGEYEKSIECYLEASKYRNLRPIDLANMAYAYYNIGNYNDALKCIDLALSMSPNKFEFLYLKGLILYKLKNFKEACKILEKACNKGKKSSDLLEILGEIFLNLEDYKKALKYFLKAYKLKPSKKNMFIIGRLYLLLGDIENAYEMFNKLLNIDPKHICRKIINDIVEIKAFIDKKIYNYIFKGLSYLEKKDYINALKIFNEMLSYEDDDFAHYYKSVISEIFEEYYKSLNYIDNSIQIIERSIFYAKKGDILRKLDSQRAIDAYMKSLEICENPYAYFGLALFYYKKDKRKAIKYFDNILEKYFEGISEDLMNMLIIYSLIGKGDLTGIKKFYEGALQYLDKIMVKYEDNPKCWRIKGYLFYKLNKYYDAEECFKKALLLDNNDVESMKFLIDIYQKLEKFDEAVDIGIKLKMLENNEKVEDMLFKLINNEKIKIDVKSPIELINIIYHKLTEVQYHFATLYRYVSKNPIKAFLYLNFIIDSGITKDLENIKVRKSLEEMLEVLKNDLTLEMFKYSENPKNYEPNKEILNFIRNKLISLGCI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTN0</accession>
    <name>N6UTN0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF2100 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06351</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95689.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTN0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01303"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1236"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016736">
        <property type="entry name" value="MJ1481-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09873">
        <property type="entry name" value="SepCysE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95689.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="196" mass="22993" checksum="2B3B3980406FAC72" modified="2013-06-26" version="1">MLRKGIETISKLRKGSVERKEVIITSGHIDVENFKKAIYYLLEADEFLYKKAPKHYLNENEAKLFCKLLIKCKESLDRVLKNFGFEIEEKEVNENALYIVYSRKLFKKLKNKYPNLAVVCTESFLDINDFKGLVPENALNGLKKKVELAKKNIEKQIKNLKPKKIYVVIEDEKDEKLYLKAKDLYNAERIDVDDLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTP4</accession>
    <name>N6UTP4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">NIL domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_06471</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95709.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593552.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTP4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01281"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1260"/>
    <dbReference type="OrthoDB" id="15347at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018449">
        <property type="entry name" value="NIL_domain"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF6">
        <property type="entry name" value="L-ASPARTATE SEMIALDEHYDE SULFURTRANSFERASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09383">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00930">
        <property type="entry name" value="NIL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="73"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95709.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="131" mass="15192" checksum="7868E40EAEC848EB" modified="2013-06-26" version="1">MRKRVYYWTHSEHINKPVISETILKTGVNINILKAKVEPQEAFLILELIGDEEKIKKALSYLSKFGDVEEIKKVIKRDYEKCVHCGCCITQCPLNIIYMDDEYNVVFKEEECVGCKNCLKACPFKAIEIID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTU5</accession>
    <name>N6UTU5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 12 14">1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase</fullName>
            <ecNumber evidence="5 12 14">5.3.1.16</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11 12">Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">hisA</name>
        <name evidence="15" type="ORF">J422_06025</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 12 14">
            <text>1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide = 5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:15469"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:58525"/>
            <dbReference type="EC" id="5.3.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12 14">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12 13">Belongs to the HisA/HisF family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.3.1.16" evidence="5 12 14"/>
    <dbReference type="EMBL" id="APMM01000048">
        <property type="protein sequence ID" value="ENN95769.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTU5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00925"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1173"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00009"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003949">
        <property type="term" value="F:1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04732">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000009">
        <property type="entry name" value="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01014">
        <property type="entry name" value="HisA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006062">
        <property type="entry name" value="His_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023016">
        <property type="entry name" value="HisA/PriA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006063">
        <property type="entry name" value="HisA_bact_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044524">
        <property type="entry name" value="Isoase_HisA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00007">
        <property type="entry name" value="1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010112">
        <property type="entry name" value="PRK13585.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43090:SF7">
        <property type="entry name" value="1-(5-PHOSPHORIBOSYL)-5-[(5-PHOSPHORIBOSYLAMINO)METHYLIDENEAMINO] IMIDAZOLE-4-CARBOXAMIDE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00977">
        <property type="entry name" value="His_biosynth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8 12 13" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 12 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="9 12 13" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="10 12 14" id="KW-0413">Isomerase</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="12">
        <location>
            <position position="15"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="12">
        <location>
            <position position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000901"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00005133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009667"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00018464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00030547"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="RuleBase" id="RU003657"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="RuleBase" id="RU003658"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN95769.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="241" mass="26830" checksum="667A61F28571AAFF" modified="2013-06-26" version="1">MSLYGEKLIIMPAIDLKDGKCVQLVQGDPNKKLFESDDVIGIAKKFEKFADCLHIVDLNAALNMGNNRELIRKIINSISIPAQVGGGIRDVDVAKEIIDFGAERIIVGTRAILDKEFIDELKSELGKKKIVLAVEEREGKVVIKGWKEKIDKTPLEIIKEFENKVGYILYTNVNVEGLLKGIDLKKIEDIVNNSKLPIIYSGGITKLDDLINLKRLNVYGAVIGSAIYKGLIKLEDLKKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTV0</accession>
    <name>N6UTV0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05928</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95774.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593029.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTV0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00574"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1154"/>
    <dbReference type="OrthoDB" id="114359at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10150">
        <property type="entry name" value="B12-dependent dehydatase associated subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010254">
        <property type="entry name" value="B12-dep_deHydtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012019">
        <property type="entry name" value="HcgB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11576">
        <property type="entry name" value="HcgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95774.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="105" mass="11608" checksum="49441E9995FA60D2" modified="2013-06-26" version="1" fragment="single">IKNIMLKFVKDAEIKMTDIDTSFADLTRMPAIFKALMAVDVENGDIYIARGRLGIPGSGAMLVILDNKGRILTASLSPPSSIHKEKIEKRIEKEIIEALNRVGIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU39</accession>
    <name>N6UU39_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Protein archease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_05464</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Activates the tRNA-splicing ligase complex by facilitating the enzymatic turnover of catalytic subunit RtcB. Acts by promoting the guanylylation of RtcB, a key intermediate step in tRNA ligation. Can also alter the NTP specificity of RtcB such that ATP, dGTP or ITP is used efficiently.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the archease family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000041">
        <property type="protein sequence ID" value="ENN95869.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592544.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU39"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01402"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1067"/>
    <dbReference type="OrthoDB" id="8831at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005509">
        <property type="term" value="F:calcium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006388">
        <property type="term" value="P:tRNA splicing, via endonucleolytic cleavage and ligation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.55.10.10">
        <property type="entry name" value="Archease domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01222">
        <property type="entry name" value="Archease_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002804">
        <property type="entry name" value="Archease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022952">
        <property type="entry name" value="Archease_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023572">
        <property type="entry name" value="Archease_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036820">
        <property type="entry name" value="Archease_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001617">
        <property type="entry name" value="PRK00407.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682">
        <property type="entry name" value="ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12682:SF11">
        <property type="entry name" value="PROTEIN ARCHEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01951">
        <property type="entry name" value="Archease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69819">
        <property type="entry name" value="MTH1598-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0106">Calcium</keyword>
    <keyword evidence="3 5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="Archease" evidence="6">
        <location>
            <begin position="2"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="137"/>
        </location>
        <ligand>
            <name>Ca(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29108"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01951"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95869.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="137" mass="16133" checksum="1BCAE3482B999AD2" modified="2013-06-26" version="1">MFDYFETTADIGVIAKGKSLEEAFREAAKGLYNIMVDIDKVNKSEVVEIEVEGEDLEELLYNFLNELLFYTDTKNIVFSDFDICIRKNNKYYLNCKAYGEKIKREHNIKEEVKAVTYHKMEIKKDSDGYKIRYIVDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUK7</accession>
    <name>N6UUK7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04765</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96024.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592003.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUK7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00972"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.932"/>
    <dbReference type="OrthoDB" id="65647at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="72"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="127"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="198"/>
            <end position="225"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96024.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="287" mass="33327" checksum="5ACB331F25958731" modified="2013-06-26" version="1">MNVIRYIRKDKFLFLMLFIWVLINIFFFLPFFKYVSNLKFSINNGLSAFSGIFLGPIYGSLAILIILFIYMITNLFQIEPFMLVPPVISTLVVGWLCEKRWRLSLHIIAFGTLILYYLIIFLKMDNYLHIPYAVIVSIAIIYIIKDKFDDYLFSSNIFKVLLSSTSLSFISIVTGHIYEKIVRIITGHKLPKICNVFINLPIIILLSLFCGIFVTVTLIALKFLIIRSPDLRDKILTTVLREEVKPIKHSVDEELLKKYGVKIPDENEQLEVLKTLAIFLSENKKLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUN5</accession>
    <name>N6UUN5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methanogen homoaconitase small subunit</fullName>
            <shortName evidence="3">HACN</shortName>
            <ecNumber evidence="3">4.2.1.114</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Homoaconitate hydratase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">hacB</name>
        <name evidence="5" type="ORF">J422_04238</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Hydro-lyase with broad substrate specificity for cis-unsaturated tricarboxylic acids. Catalyzes both the reversible dehydration of (R)-homocitrate ((R)-2-hydroxybutane-1,2,4-tricarboxylate) to produce cis-homoaconitate ((Z)-but-1-ene-1,2,4-tricarboxylate), and its hydration to homoisocitrate ((1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate). Is also able to hydrate the analogous longer chain substrates cis-homo(2)-aconitate, cis-homo(3)-aconitate. All these reactions are part of the biosynthesis pathway of coenzyme B.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(2R)-homocitrate = (2R,3S)-homoisocitrate</text>
            <dbReference type="Rhea" id="RHEA:32303"/>
            <dbReference type="ChEBI" id="CHEBI:15404"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
            <dbReference type="EC" id="4.2.1.114"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Organic acid metabolism; 2-oxosuberate biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotetramer of 2 HacA and 2 HacB proteins.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the LeuD family. LeuD type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.114" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96049.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591726.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUN5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00675"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.829"/>
    <dbReference type="OrthoDB" id="6505at2157"/>
    <dbReference type="UniPathway" id="UPA00919"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004409">
        <property type="term" value="F:homoaconitate hydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019298">
        <property type="term" value="P:coenzyme B biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01577">
        <property type="entry name" value="IPMI_Swivel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.19.10">
        <property type="entry name" value="Aconitase, domain 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01032">
        <property type="entry name" value="LeuD_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015928">
        <property type="entry name" value="Aconitase/3IPM_dehydase_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000573">
        <property type="entry name" value="AconitaseA/IPMdHydase_ssu_swvl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033940">
        <property type="entry name" value="IPMI_Swivel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050075">
        <property type="entry name" value="LeuD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011827">
        <property type="entry name" value="LeuD_type2/HacB/DmdB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040604">
        <property type="entry name" value="HacB_Meth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02087">
        <property type="entry name" value="LEUD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43345:SF2">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43345">
        <property type="entry name" value="3-ISOPROPYLMALATE DEHYDRATASE SMALL SUBUNIT 2-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00694">
        <property type="entry name" value="Aconitase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52016">
        <property type="entry name" value="LeuD/IlvD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aconitase A/isopropylmalate dehydratase small subunit swivel" evidence="4">
        <location>
            <begin position="44"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="YLRT" evidence="3">
        <location>
            <begin position="22"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="site" description="Critical for substrate specificity" evidence="3">
        <location>
            <position position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01032"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF00694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96049.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="159" mass="17721" checksum="79ED4B5BC339BAA9" modified="2013-06-26" version="1">MRGIAYKLGDDVDTDAIIPGPYLRTTDPNELARYCLYGIDKDFYKKAKGKIIVAGENFGCGSSREQAVLAIKYAGVKAVVAKSFARIFYRNAINNGLYVITANTDEINDGDELFIDLKNEKIIINNKKTISCKIPKGIEKEILEAGGLINYYKKKKVRT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUN8</accession>
    <name>N6UUN8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 4">IMP cyclohydrolase</fullName>
            <ecNumber evidence="3 4">3.5.4.10</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">IMP synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Inosinicase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">purO</name>
        <name evidence="6" type="ORF">J422_04263</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the cyclization of 5-formylamidoimidazole-4-carboxamide ribonucleotide to IMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:18445"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="EC" id="3.5.4.10"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the archaeal IMP cyclohydrolase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.10" evidence="3 4"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96054.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591736.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUN8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00670"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.834"/>
    <dbReference type="OrthoDB" id="92928at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00135"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003937">
        <property type="term" value="F:IMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.20">
        <property type="entry name" value="Inosine monophosphate cyclohydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00705">
        <property type="entry name" value="IMP_cyclohydrol"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010191">
        <property type="entry name" value="IMP_cyclohydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020600">
        <property type="entry name" value="IMP_cyclohydrolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036795">
        <property type="entry name" value="IMP_cyclohydrolase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003167">
        <property type="entry name" value="PRK04151.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01922">
        <property type="entry name" value="purO_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07826">
        <property type="entry name" value="IMP_cyclohyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004866">
        <property type="entry name" value="IMP_cclhdr_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75569">
        <property type="entry name" value="Archaeal IMP cyclohydrolase PurO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 3" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Inosine monophosphate cyclohydrolase-like" evidence="5">
        <location>
            <begin position="2"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="NCBIfam" id="TIGR01922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF07826"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96054.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="200" mass="22568" checksum="7492B43BD68349AD" modified="2013-06-26" version="1">MYIGRFLVAGKTKNGAPFIAYRVSSTSFPNRIAKINGNTVAIIPKDLNDIFKNPYICYNCIKVVNNTVVASNGSHTDFIAEKLSYGKRDALATVLLAMDYEKDNYKTPRIAGILDREECYLAYIKDDELRVKKVYLKDGKGYYLGVYNSCEINEKQVIEIEGESAEEIAKYLLEYEEFEYPVASAVALIKEKIELAVANK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUS8</accession>
    <name>N6UUS8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04483</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96094.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUS8"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96094.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="69" mass="8591" checksum="937C2CD249269F97" modified="2013-06-26" version="1">MQLVQETLQRFNWNDIRQKADVTGRDVLHHQKPEKIHPLNRYLKNWREITEPFTISRRKLWSYLKKTIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV54</accession>
    <name>N6UV54_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Phosphate-specific transport system accessory protein PhoU</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_03238</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Plays a role in the regulation of phosphate uptake.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 7">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the PhoU family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96244.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590940.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV54"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01035"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.634"/>
    <dbReference type="OrthoDB" id="7738at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006817">
        <property type="term" value="P:phosphate ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.58.220:FF:000004">
        <property type="entry name" value="Phosphate-specific transport system accessory protein PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02135">
        <property type="entry name" value="phoU_full"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF3">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003107">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 7" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PhoU" evidence="9">
        <location>
            <begin position="15"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="9">
        <location>
            <begin position="120"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="1"/>
            <end position="28"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRNR" id="PIRNR003107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96244.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="222" mass="25934" checksum="6B8E4D169B4C28F4" modified="2013-06-26" version="1">MRRFEAIIKELEGDLMEMANLCKEQTENAIKVFMEGDKNNAKIIRKKDVDIDLMEINIEDKCIKSLALYQPVAEDLREIITVLKISSKLEKVGDNASKICKLLLKSDIEGKRYNELLIVMKNYLLDMLDEAMDAFKNRDEKLAIEVYNMDKKLHELFEQLYRSMLTKIIENPNKITLYTEIIFAGKYLERSGNIVASIGDRIVFMLTGERIKEWELEEEIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVD7</accession>
    <name>N6UVD7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03129</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96319.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590780.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVD7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00172"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.614"/>
    <dbReference type="OrthoDB" id="85577at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007503">
        <property type="entry name" value="DUF530"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04409">
        <property type="entry name" value="DUF530"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="50"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96319.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="466" mass="55287" checksum="5C21DCF93FDC3E35" modified="2013-06-26" version="1">METSNLIKEANEFLDNIKIDFEKNNLDDIIKKLKDNIKILQELKSKMEFLEFDNPYKNVGKVKLVDSESLQELATYSSYLRRIAGEKKGVLERVKHALTAHKIALAHLVDEIGNKRLPPNLPLDGAYKEHLFELPPYLISIYKEFLDMLEPKGKGVLTSYTVSLIIFKNGKREFKRVRVEDKNYEKYIKEKYGNAIITSIKRNYSKNKIIDDQYVRRVLAIGYIKAYKTEVEREIEKRLKKLLSKEERNYLEKYYMILNKFKDEKEEVCGGILDIRQLEEKKLKELELKRILENEGLIEDEELIIPLKRAIEIRNKVSKEVAKEIIFREFSEDVFKFYLYKTPDERARSFLFPSIMVSPNKGYLFWMDKKKADLLDTKFKLEELLPKFNIGLKNIGGVLLYLFYDWDMVEKYKFKKEEIEDLLKKIALIEPIKEILKDKIDIKKLEKFGKVKKEKTKKFLSLLKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVS6</accession>
    <name>N6UVS6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02400</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96439.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVS6"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002825">
        <property type="entry name" value="Pept_S49_ser-pept_pro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35984">
        <property type="entry name" value="PERIPLASMIC SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35984:SF1">
        <property type="entry name" value="PERIPLASMIC SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01972">
        <property type="entry name" value="SDH_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="85"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96439.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="85" mass="9802" checksum="AF0290B6BB49DF27" modified="2013-06-26" version="1" fragment="single">MRRLKCIKDLERERKSRVIAMIHRQEVLSFLGIPIYKYITIEDSEEILRAIRLTPEDMPIDLIIHTPGGIALAAEQIALALKEHP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6UW22</accession>
    <name>N6UW22_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01885</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96524.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590135.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UW22"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00225"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.371"/>
    <dbReference type="OrthoDB" id="74957at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012021">
        <property type="entry name" value="UCP005278"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005278">
        <property type="entry name" value="UCP005278"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96524.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="236" mass="26227" checksum="646C828221226720" modified="2013-06-26" version="1">MYIVSFGVDIADNDVEANKDINDLVDKEVRERLARYGIKGRISNVTGDDIVITSLTPKDKLELNNKIIFETLKKYAKSFDDLNGVAEKPEKAKEGVSYAITEALSDYGDAIIVSFDTYGGEKMVNEMALTVKEICEKLNYNVDSSVTEGPKYIHGVGYTGKETDDPVVVIAVRELEDIKKLSCIIFGTLLSFDNLYFVKSGEKINILPPGVIYTMSAFLNGNIIDLYDGIKKRLII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWA5</accession>
    <name>N6UWA5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF2666 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01615</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589982.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWA5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00450"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.318"/>
    <dbReference type="OrthoDB" id="86044at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022620">
        <property type="entry name" value="DUF2666"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10869">
        <property type="entry name" value="DUF2666"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2666" evidence="1">
        <location>
            <begin position="2"/>
            <end position="135"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF10869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="141" mass="16367" checksum="DA400C0C4AA37549" modified="2013-06-26" version="1">MEEKIEFLAKHKNWFVVKKLKIDEHTEDIEIARLLASIGETVDNKFIEYLPFDVNKLYEIADSIYQKKKGKIKEEDIINALKKLKSPATTKKLNEIDASKEGKEILKIMLNNIVLKRLGIETKVPAKLIEKYIEKKNLGEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="57" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWD0</accession>
    <name>N6UWD0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Threonine--tRNA ligase</fullName>
            <ecNumber evidence="14">6.1.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="14">Threonyl-tRNA synthetase</fullName>
            <shortName evidence="14">ThrRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="primary">thrS</name>
        <name evidence="16" type="ORF">J422_01398</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12 14">
            <text>tRNA(Thr) + L-threonine + ATP = L-threonyl-tRNA(Thr) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24624"/>
            <dbReference type="Rhea" id="RHEA-COMP:9670"/>
            <dbReference type="Rhea" id="RHEA-COMP:9704"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57926"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78534"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="14">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="14">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 14">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="13">Belongs to the class-II aminoacyl-tRNA synthetase family. Archaea-specific ThrRS editing domain subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.3" evidence="14"/>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96614.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589943.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWD0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00009"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.274"/>
    <dbReference type="OrthoDB" id="372136at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004829">
        <property type="term" value="F:threonine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006435">
        <property type="term" value="P:threonyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00860">
        <property type="entry name" value="ThrRS_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.800:FF:000001">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.80.10:FF:000004">
        <property type="entry name" value="Threonine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.80.10">
        <property type="entry name" value="D-tyrosyl-tRNA(Tyr) deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00184">
        <property type="entry name" value="Thr_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023509">
        <property type="entry name" value="DTD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002320">
        <property type="entry name" value="Thr-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015011">
        <property type="entry name" value="Threonyl-tRNA_syn_edit_dom_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047246">
        <property type="entry name" value="ThrRS_anticodon"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003068">
        <property type="entry name" value="PRK03991.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00418">
        <property type="entry name" value="thrS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451:SF44">
        <property type="entry name" value="THREONINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11451">
        <property type="entry name" value="THREONINE-TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08915">
        <property type="entry name" value="tRNA-Thr_ED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01047">
        <property type="entry name" value="TRNASYNTHTHR"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 14" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 14" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 14" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 14 16" id="KW-0436">Ligase</keyword>
    <keyword evidence="5 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 14" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 14" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 14" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 14" id="KW-0820">tRNA-binding</keyword>
    <keyword evidence="7 14" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="15">
        <location>
            <begin position="219"/>
            <end position="481"/>
        </location>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="282"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="14">
        <location>
            <position position="454"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00049515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00060816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN96614.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="608" mass="71319" checksum="114E6D5CD0D07B10" modified="2013-06-26" version="1">MKMLLIHADYLEFEAKEKTKIAEETDILKGRMEDCLVCFIAVERGDNKNVIKPAIEEIDKVAKQLKVNNIVLYPYAHLSSDLSDPETAVNILKLLEEELKSRYNVLRAPFGWYKSFKLSCKGHPLSELSRKIEPKEEEEKKEVKSKIYLIKDNEIIEINEKNLDIIEDKNLLALAKHELGIKEKADKEPYHIKFIREKDICDHEPSSDPGHFRWYPKGKLIRDLLAEYVYNLVINMGAMPVETPVMYDLSNRAIKEHADKFGERQYRFRQGNKELMLRFAACFGQFMIKKDMFLQPKYLPLKLYELSTYSFRYEQRGELVGLKRLRAFTMPDMHTVCLNLDQAIEEFERQFWTCLKTGEDLGVEYSVIFRFTRDFFEEHKDWFLKIAKEYKEKYNKDLLLEILEERKHYWVGKVDIAAIDSLNRPIENPTVQIDVESARRFGIKVGDIHPIILHCSPTGSLERVICSIIEEAYKNVDKKAPTLPLWLSPIQVRIIPVSEKYNDYAIEIAEILRRNNIRADVDDRDETVGKKIRDANREWIPYIVVVGEEEVKSNVLTVTVREKSTLKSSYKEKKTLEELINEIKEKVKGYPYKPLPLPIRCSLQPKFH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWG8</accession>
    <name>N6UWG8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PP-loop domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_00986</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000006">
        <property type="protein sequence ID" value="ENN96669.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589865.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWG8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01021"/>
    <dbReference type="OrthoDB" id="33422at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002144">
        <property type="term" value="C:cytosolic tRNA wobble base thiouridylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002143">
        <property type="term" value="P:tRNA wobble position uridine thiolation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000541">
        <property type="entry name" value="Ncs6/Tuc1/Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011063">
        <property type="entry name" value="TilS/TtcA_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035107">
        <property type="entry name" value="tRNA_thiolation_TtcA_Ctu1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054306">
        <property type="entry name" value="TtuA-like_LIM_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00269">
        <property type="entry name" value="TIGR00269 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807">
        <property type="entry name" value="ATPASES OF THE PP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11807:SF12">
        <property type="entry name" value="CYTOPLASMIC TRNA 2-THIOLATION PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01171">
        <property type="entry name" value="ATP_bind_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22082">
        <property type="entry name" value="TtuA_LIM_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004976">
        <property type="entry name" value="ATPase_YdaO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="2-thiouridine synthetase TtuA-like N-terminal LIM" evidence="4">
        <location>
            <begin position="5"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="domain" description="tRNA(Ile)-lysidine/2-thiocytidine synthase N-terminal" evidence="3">
        <location>
            <begin position="46"/>
            <end position="224"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <begin position="50"/>
            <end position="52"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="165"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR004976-51"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96669.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="288" mass="33667" checksum="92E317824BE6BE8C" modified="2013-06-26" version="1" fragment="single">MLCSCGKVAVYYQRYANKYLCEECFKKSVEKRVKKTIKDVIRNNVKIAIAISGGKDSLVMAKLLKNLFKNIPNCKLTCFFVNEGIRGFRDKAIKYVKEFCKREELDLKIISFKEEIGYNLDEILEHKDKLNIGKPCSFCGVLRRYILNKYALGYDYLAIGHNLDDMAQTILMNYVEGNIKNIVHFGKDIEDKKFVKRIRPLKYIPEEEVKLYADIEGIVYQSEPCPYSSLSYRHRMKEVIKTLENIDPKVKFSIVKGYERLLNYICYKEEIKRCKICGFPSNNEICKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWH5</accession>
    <name>N6UWH5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glutamine amidotransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01036</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000006">
        <property type="protein sequence ID" value="ENN96679.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589875.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWH5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01011"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.202"/>
    <dbReference type="OrthoDB" id="350529at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01908">
        <property type="entry name" value="YafJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026869">
        <property type="entry name" value="EgtC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017932">
        <property type="entry name" value="GATase_2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42824:SF1">
        <property type="entry name" value="GLUTAMINE AMIDOTRANSFERASE YAFJ-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13230">
        <property type="entry name" value="GATase_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51278">
        <property type="entry name" value="GATASE_TYPE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glutamine amidotransferase type-2" evidence="2">
        <location>
            <begin position="2"/>
            <end position="247"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51278"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96679.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="347" mass="40919" checksum="0C8C2C0128F8791F" modified="2013-06-26" version="1">MCELLGICFNKKVNVRLSLNSFKQRSDMHPNGWGIAFYPDEFVRVIKEPIKMNDALLANCIRWINIKSKIFIAHIRKASAGKVSYVNTHPFVRKLDDKEIVFAHNGTLYDYWDLELDEFYPIGETDSEYAFCYLLSQIKNNKINYGEGFDALLDILYEINSCGPFNCLFSDGEYLFAYKDYEGKRDLYFLKRTPPYKPIKLIDEDYTVNLGDIKNPKEEGFIIATNPLTNENWQHFKNGELIVFKNWEMIYSNKRLSDLELKILKILRESPHRVSLRDIIKNLNDLPKNIEYDESVVKIGIRSLLDKEYIKQDGRDTVNWDDLEATFYTKPKRRAEIDKKLKELRWL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWJ9</accession>
    <name>N6UWJ9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Hydantoinase A/oxoprolinase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00621</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96704.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589804.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWJ9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00909"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.119"/>
    <dbReference type="OrthoDB" id="148086at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.190">
        <property type="entry name" value="conserved archaeal protein q6m145"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043129">
        <property type="entry name" value="ATPase_NBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002821">
        <property type="entry name" value="Hydantoinase_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002756">
        <property type="entry name" value="MfnF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053660">
        <property type="entry name" value="MfnF_synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040623">
        <property type="entry name" value="MfnF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03123">
        <property type="entry name" value="one_C_unchar_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01968">
        <property type="entry name" value="Hydantoinase_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53067">
        <property type="entry name" value="Actin-like ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hydantoinase A/oxoprolinase" evidence="1">
        <location>
            <begin position="47"/>
            <end position="314"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96704.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="319" mass="36136" checksum="F900945DBE361F72" modified="2013-06-26" version="1">MLGIDIGGANTKIVDIDNNKVYHIYFPIWKMKDRLVDLLKDFSDDKVALVMTAELADCYKTKREGVEDILNKVGSVFKEVYVLDVDGNFLTLDEAKRNYLKVSASNWMASAKFISKFYDNCIFIDMGSTTTDIIPIKDKKILAKKTDLERLMENQLLYVGALRTNLSFLTNRVLFRGKETNISSEYFAITADICRILGKIDENDYSCETPDGGGKDLESCYIRLARVLCADIEMVSKDELYSLAEQFYNKILNLIRENVNKVASRYGIKDVVICGQGEDIIKDALKDYNVISIKEKYGKEISLAFPAFAVGKLLEVEIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWU0</accession>
    <name>N6UWU0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Pyridoxal phosphate enzyme</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_00130</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 3">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589715.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWU0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01142"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.26"/>
    <dbReference type="OrthoDB" id="67852at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004125">
        <property type="term" value="F:L-seryl-tRNA(Sec) selenium transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020033">
        <property type="entry name" value="PyrdxlP-dep_transferase_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018319">
        <property type="entry name" value="SelA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055177">
        <property type="entry name" value="UPF0425_MJ0158-like_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03576">
        <property type="entry name" value="pyridox_MJ0158"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32328">
        <property type="entry name" value="L-SERYL-TRNA(SEC) SELENIUM TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32328:SF0">
        <property type="entry name" value="L-SERYL-TRNA(SEC) SELENIUM TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03841">
        <property type="entry name" value="SelA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22583">
        <property type="entry name" value="UPF0425_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UPF0425" evidence="4">
        <location>
            <begin position="274"/>
            <end position="330"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="3">
        <location>
            <position position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR618319-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF22583"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="348" mass="38885" checksum="2499F1952AAADFDC" modified="2013-06-26" version="1">MKDIERLERAKKLILKILNEKGREYLYDLSGLSGGFLIEENDKELLTTYIGSQYFLEKVNDLGLKHLGGSVGDRCIALNRTTSAILATVLTLKPKHIYHYLPELPGHPSIRRAAEIVNAYYEESDNLNILKKANENSLAVITGTTMDLKVINLELFKEAIEIAKEKSAIILVDDASGARIRLLYGQPPALKLGADLVVTSTDKLMHGPRAGLLAGKKEIVDEIYITALKYGLEAQPPILAGIYRALEKFNLDHLKKVFNRAKNLDLSKLDELDVNYERTATGFVVKDVNEEVGFYLLEKYGILTILVFGAPGSSRNLRIDLFSNDAERLSDEYIINAVVDAIKRCNYV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6V006</accession>
    <name>N6V006_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">SufBD protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06847</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95618.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594244.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V006"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01078"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1328"/>
    <dbReference type="OrthoDB" id="372168at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016226">
        <property type="term" value="P:iron-sulfur cluster assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055346">
        <property type="entry name" value="Fe-S_cluster_assembly_SufBD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000825">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037284">
        <property type="entry name" value="SUF_FeS_clus_asmbl_SufBD_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508">
        <property type="entry name" value="FES CLUSTER ASSEMBLY PROTEIN SUF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30508:SF6">
        <property type="entry name" value="UPF0051 PROTEIN MJ0034"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01458">
        <property type="entry name" value="SUFBD_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101960">
        <property type="entry name" value="Stabilizer of iron transporter SufD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SUF system FeS cluster assembly SufBD core" evidence="1">
        <location>
            <begin position="81"/>
            <end position="309"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01458"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95618.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="319" mass="35869" checksum="8CE896033E0830F8" modified="2013-06-26" version="1">MKEEILRDIEFLKYTMKNPEEIVHGKGARIIIKEGKILDIKETEGIKIEGKEEDGKILAKIIVKKGYKFKEPIHMCTGITEDNVSQIIDVEIILEDDSEIMLLSHCSFPKGKKLKHIMNGNIKIGKNAKFIYKETHYHGEEGDILVKPTMKVNIGERGVYISEFNLTKGRIGILDIHQEIEADKEALIDIITKTYAIKDDKVNVDEIVRLNGENAKCMIRGRGAGKDNSKITLKFKIEGNAPYCKGHIDCAEIIKDKAEVESIPIVLVRDEKARITHEAAIGSVDKKQLETLMAKGLDEEEATEVIIKGMIGDDENFAI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6V076</accession>
    <name>N6V076_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Type II secretion system protein E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06491</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the GSP E family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593557.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V076"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01277"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1263"/>
    <dbReference type="OrthoDB" id="33500at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01130">
        <property type="entry name" value="VirB11-like_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.450.380">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001482">
        <property type="entry name" value="T2SS/T4SS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050921">
        <property type="entry name" value="T4SS_GSP_E_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486">
        <property type="entry name" value="TWITCHING MOTILITY PROTEIN PILT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30486:SF15">
        <property type="entry name" value="TYPE II_IV SECRETION SYSTEM ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00437">
        <property type="entry name" value="T2SSE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Bacterial type II secretion system protein E" evidence="2">
        <location>
            <begin position="173"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00437"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="512" mass="58684" checksum="593C85163850D78C" modified="2013-06-26" version="1">MGLIDKIKKDKKIIVEDKPSSILKKPIKKEEDILDVYDVNVDEITFNVIIKKEEGYTYYLIPEIEKINESLSKLSKEHFDLIRMQIGDLGLVTYEQIKHFINDFSIKYNLKLPYIESLAKFFYLISGRFGLLEIPINDERLEEIMINGYNYPVYVFHRKHQMCETNIILDRNEVDRIIESIANLVNRPIDSRTPMLDAFLPDGSRVNATTSDICMNGATLTIRKFSKDPLTVIDLINFGTLDIDTAAFLWQAVEGYFGAKPANTLIVGGTGSGKTTLLNVLSMFSMYNERIITIEDTPELQIPHKHVIKLVTRPARPGMPEYEVTMDDLIKNALRMRPDRIFVGEVRGEEAHSLLVAMNTGHDGCSGTLHANSADEAILRLTSPPMNVPKIMLTALNFIINIQRIRRAGKTIRRILGIVEVVKGVGEGQEIAKTTLYEYNGLKDILEKRGICLWEEEVCEIAGISRDELLRDREFRKRVLNYLYRNNIRDLESVGKYIMKYQENPEKLMKMI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0A9</accession>
    <name>N6V0A9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">O-phosphoserine--tRNA(Cys) ligase</fullName>
            <shortName evidence="6">O-phosphoserine--tRNA ligase</shortName>
            <ecNumber evidence="6">6.1.1.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Non-canonical O-phosphoseryl-tRNA(Cys) synthetase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">O-phosphoseryl-tRNA(Cys) synthetase</fullName>
            <shortName evidence="6">SepRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">sepS</name>
        <name evidence="8" type="ORF">J422_06115</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the attachment of O-phosphoserine (Sep) to tRNA(Cys).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>tRNA(Cys) + O-phospho-L-serine + ATP = O-phospho-L-seryl-tRNA(Cys) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25678"/>
            <dbReference type="Rhea" id="RHEA-COMP:9661"/>
            <dbReference type="Rhea" id="RHEA-COMP:9719"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57524"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.27"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer. Interacts with SepCysS.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the class-II aminoacyl-tRNA synthetase family. O-phosphoseryl-tRNA(Cys) synthetase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.27" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95753.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593249.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0A9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00543"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1191"/>
    <dbReference type="OrthoDB" id="145125at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043816">
        <property type="term" value="F:phosphoserine-tRNA(Cys) ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043039">
        <property type="term" value="P:tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.250.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01674">
        <property type="entry name" value="Sep_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005246">
        <property type="entry name" value="O-Pseryl-tRNA(Cys)_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041590">
        <property type="entry name" value="SepRS_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00470">
        <property type="entry name" value="sepS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18006">
        <property type="entry name" value="SepRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="3 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="7">
        <location>
            <begin position="48"/>
            <end position="342"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="187"/>
            <end position="189"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="232"/>
            <end position="234"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="274"/>
            <end position="275"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="318"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95753.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="538" mass="62860" checksum="017D39AFA71F51A6" modified="2013-06-26" version="1">MRFNIKEVLNLAERDFEKAWRDSKKYIKKEINKRYPRINIEYGKPHPVMEVIQRLREAYLRMGFEEVINPIIVDELEVYKQFGSEAMAVLDRCFYLAGLPRPDVGLSKEKIEIIKNLNIDIDDNKAERLRETLHKYKKGEVDGDDLIYEISKALNIDNELALKVLDKAFPEFKDLKPEASSLTLRSHMTSGWFITLSHLIKKRDLPIKLFSIDRCFRREQREDRSHLYSYHSASCVIADEDVSEDEGKAVAEALLKQFGFTKFKFKLDEKKSKYYTPETQTEVYAFHPKLNDWIEIATFGVYSPVSLSKYNIDVPVMNLGLGVERLTMVLYNYDDVRRMVYPQFYELILDDIEIARAIKIDKTPILDEFHDLTCEIIDKCISHKDDIAPTSITFEKTLKFNSEKRRIIIKIFENEEGKKLLGPSVLNEIYVYNGNIYGIPKNLEGIKEEYRDVLKDVKDHGVSTGIRYIDGVIYKLTSKIEEALVSNIDRFNFRVTIVRNLSDINLKVDDKVMKYLMRNEKIIDVRGPVFLNAEVIIH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0B2</accession>
    <name>N6V0B2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Large ribosomal subunit protein P1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rpl12p</name>
        <name evidence="4" type="synonym">rpl12</name>
        <name evidence="6" type="ORF">J422_06150</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Forms part of the ribosomal stalk, playing a central role in the interaction of the ribosome with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Part of the 50S ribosomal subunit. Homodimer, it forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors. Forms a heptameric L10(L12)2(L12)2(L12)2 complex, where L10 forms an elongated spine to which the L12 dimers bind in a sequential fashion.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the eukaryotic ribosomal protein P1/P2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95758.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593259.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0B2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00549"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1198"/>
    <dbReference type="OrthoDB" id="3337at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006414">
        <property type="term" value="P:translational elongation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05832">
        <property type="entry name" value="Ribosomal_L12p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.1410:FF:000002">
        <property type="entry name" value="60S acidic ribosomal protein P2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.1410">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01478">
        <property type="entry name" value="Ribosomal_L12_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038716">
        <property type="entry name" value="P1/P2_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027534">
        <property type="entry name" value="Ribosomal_P1/P2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022295">
        <property type="entry name" value="Ribosomal_P1_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03685">
        <property type="entry name" value="ribo_P1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696">
        <property type="entry name" value="60S ACIDIC RIBOSOMAL PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45696:SF10">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN P1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00428">
        <property type="entry name" value="Ribosomal_60s"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 4 6" id="KW-0689">Ribosomal protein</keyword>
    <feature type="region of interest" description="Disordered" evidence="5">
        <location>
            <begin position="68"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="5">
        <location>
            <begin position="70"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005436"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95758.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="101" mass="10615" checksum="E9A071A29D90189C" modified="2013-06-26" version="1">MEYIYAALLLHSAGKEINEENLKAVLQAAGIEVDEARIKALVAGLEGVNIDEAIANAAMPVAVAAPAPAVEEKKEEAKEEKKEEKKEEETAAVAGLAALFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0C0</accession>
    <name>N6V0C0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Phenylalanine--tRNA ligase alpha subunit</fullName>
            <ecNumber evidence="11">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Phenylalanyl-tRNA synthetase alpha subunit</fullName>
            <shortName evidence="11">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 14" type="primary">pheS</name>
        <name evidence="14" type="ORF">J422_06020</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="16">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per tetramer.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 11">Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha subunit type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000048">
        <property type="protein sequence ID" value="ENN95768.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593141.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0C0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00924"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1172"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00496">
        <property type="entry name" value="PheRS_alpha_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000095">
        <property type="entry name" value="Phenylalanine--tRNA ligase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00282">
        <property type="entry name" value="Phe_tRNA_synth_alpha2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004529">
        <property type="entry name" value="Phe-tRNA-synth_IIc_asu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022917">
        <property type="entry name" value="Phe_tRNA_ligase_alpha_bac/arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002319">
        <property type="entry name" value="Phenylalanyl-tRNA_Synthase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00468">
        <property type="entry name" value="pheS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003210">
        <property type="entry name" value="PRK04172.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538:SF40">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE ALPHA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11538">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01409">
        <property type="entry name" value="tRNA-synt_2d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 11" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="7 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="12" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 11 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="8 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="13">
        <location>
            <begin position="227"/>
            <end position="475"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="12">
        <location>
            <begin position="69"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="319"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="402"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="404"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>ligand shared with heterodimeric partner</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="427"/>
        </location>
        <ligand>
            <name>L-phenylalanine</name>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00282"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN95768.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="475" mass="56313" checksum="ADDF39F85D42CA0F" modified="2013-06-26" version="1">MLHIDEKRLLKVFQDKGKEILDINEVQLPKEKIMRVSLWLKGKNLLDIEEKKREKVKVVNKKPFPERKIANYLKEIGKNEIEIKELKNILDKEEINAALGAIKRKGIAKVEKGKIIFDNLDYYDEEEEILKRLEGKYLDELSEEEKKYLNILKKRGYLDIDEEKEIKLKLTEKGKELIKEPIVIEEEINQLNREIIISGKWRKAKIREYDIKAPTKEIYPAKLHPLTRIIREVKEILLSMGFKEVKSPLVETEFWNFDVLFEPQDHPAREMHDTFFLKYPCKGEVPKDLLERVKEVHLKYWRIFDKSVSERLILRTHTTAATIRYLASLEEDEKVKPHKVFCIDRVFRNEAIDYKHLPEFYQCDGIIMADNVNFNNLIGVLREFLKRLGFEKVRFRPAYFPFTEPSLEAEIYLENKGWLEILGAGIFRPELLEPLGIKKPVLAWGIGFSRLAMLKYGLTDIRDLHKNDLNWLRRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0E7</accession>
    <name>N6V0E7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Helicase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_05878</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000045">
        <property type="protein sequence ID" value="ENN95798.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592898.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000045.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0E7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00848"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1146"/>
    <dbReference type="OrthoDB" id="6396at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd18011">
        <property type="entry name" value="DEXDc_RapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18793">
        <property type="entry name" value="SF2_C_SNF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10810">
        <property type="entry name" value="Tandem AAA-ATPase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057342">
        <property type="entry name" value="DEXDc_RapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024975">
        <property type="entry name" value="NOV_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038718">
        <property type="entry name" value="SNF2-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049730">
        <property type="entry name" value="SNF2/RAD54-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000330">
        <property type="entry name" value="SNF2_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766">
        <property type="entry name" value="DNA ANNEALING HELICASE AND ENDONUCLEASE ZRANB3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45766:SF6">
        <property type="entry name" value="SWI_SNF-RELATED MATRIX-ASSOCIATED ACTIN-DEPENDENT REGULATOR OF CHROMATIN SUBFAMILY A-LIKE PROTEIN 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13020">
        <property type="entry name" value="NOV_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00176">
        <property type="entry name" value="SNF2-rel_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 7" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="5">
        <location>
            <begin position="46"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="6">
        <location>
            <begin position="389"/>
            <end position="557"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95798.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="999" mass="117124" checksum="2D8FABC9132E6AD8" modified="2013-06-26" version="1">MQDKIKNYLTKKIFLHDPLLFFYALNHPTSKKEIKPFIHQIHLLYNAMLLRPVRFLIADEIGLGKTIESLAIARYLELKEGIKKILVLTPKILREQWESEIKRVGGIPKIIKDGNDISRIKNSFQYNYDSAVYIIISIDLAKRYVDKILQIDWDLVIVDEAHNITKNTQRGRLVEKLSEKVKNILLLSATPHRGNPQDYLYRLKMLDPTLINDFDKLNAKNFYRRTHDILVFRRTKKLVNTLENKRVFKDCQFNAVVVDISEEEKRFFDLLENTLKNILKNTKRNSPIALLAVILGKRASSSYKAAIETLNRIIKTNILKTDDIDVSEDIQNLFSLSFEEMEFEEYREISDIINNIVDKYSPYLSREQKELFENLLKTTTNIIIKKDNKIDALAKIIAEHIKNNEKIVVFTEYVDTLKYLKENLHKYLNNYGIKIDSNEILTLSGEDKNRIENITEEINRKFEDHGKILLATDVASEGLNLQIASVLINYDSPWSPIKLEQRIGRVWRLGQEKDVSIYNIFLSNRMDLELLNSLYKKIMNIKTALDNQLAIGKEIYIVNAENLFNLEEFACIKNLSEYDIIISAIMGNLNSYTEEIVKTLKIIRNKLKMMSIFPEEHAEEIKNEVLNVIQNEDYLKIEKIEEILRDYQDKVLNKNIHTQSGLYQIIKSKNDEDLQISRIVVKNPVKNREYLFFVKVLNEDDEVVWEIPLMVSRQEKNIKLVTSVELLEYLTKIFSKDLFFTEYIEKPYEKQILAIKGNILTYVRSIVSNIGKRISNYENLKDLGLKDDKLFKNLKISISDPIIIDYLPENEFSIGKIIPLEIVDTLDKDNIILPDENDLKAMDRNFLPLEELVKIEKLAMNVIMNLEEKRLASKYGYENKGKVWDVLDVSLHEHYDIKVIENGDEKYIEVKGHKGLLPIAELTEAEYRFAMENEDKYYLYIVCNLGKDEKNLIVFEIHKPLNKEHRRIYLIKNGEKIDVSKYYNNINITEKKRYLFKLA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0G2</accession>
    <name>N6V0G2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_05738</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95813.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0G2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01257"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1119"/>
    <dbReference type="OrthoDB" id="49555at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034471">
        <property type="entry name" value="GDGT/MA_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034474">
        <property type="entry name" value="Methyltransferase_Class_D"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056488">
        <property type="entry name" value="Zn_ribbon_HMPTM"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045702">
        <property type="entry name" value="rSAM_GDGT_ether"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43306">
        <property type="entry name" value="7,8-DIHYDRO-6-HYDROXYMETHYLPTERIN DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43306:SF1">
        <property type="entry name" value="7,8-DIHYDRO-6-HYDROXYMETHYLPTERIN DIMETHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23545">
        <property type="entry name" value="Zn_ribbon_HMPTM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00385">
        <property type="entry name" value="7_8-dihydro-6-hydroxymethylpte"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01100">
        <property type="entry name" value="methyltransferase_(Class_D)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="84"/>
            <end position="306"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95813.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="489" mass="55958" checksum="EE9ECA9D468AC5BE" modified="2013-06-26" version="1">MEIIKETLSLCPVCLKRIPAIVYEENNKIFIKKSCPEHGEFKDLYWSDARLYKKYNKYEFVNKIDFHQTEIDKGCPYDCGLCPAHKSSTILANIDVTNRCNLNCPICFANANKTGKIYEPSFEEIKNMMINLRNEIPPTPAIQFAGGEPTVRQDLPELIKLARDIGFVHIQLATNGIKLKNINYLKKIKEAGLSTIYLQFDGISEKPYLIARGKNLFPIKDKVIKNCRELEFNSVVLVPTLVKDVNDCEVGGIIDYAVKNVDVVRGVNFQPVSFSGRVDEDVLLNGRITIPDFIRLVEEQTSLTEEDFYPVPSVAPISIFVEKLFNKKKPTLSAHQHCGVATYVFVDDGKMIPITRFIDVEGFLELIKEYIEELDYNNKLKDIKVVLEIGSKLPSLIDLKNAPKYINIKKIISLIVNILKGDYDALAKFHYNTLMISCMHFMDAYNFDVDRVKRCCIHYAIPDGRIIPFCSYNTIHRKEVEEKFSKPKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0M1</accession>
    <name>N6V0M1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05559</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000042">
        <property type="protein sequence ID" value="ENN95863.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592616.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0M1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00753"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1085"/>
    <dbReference type="OrthoDB" id="108920at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055834">
        <property type="entry name" value="DUF7411"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011155">
        <property type="entry name" value="PRK14561.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24167">
        <property type="entry name" value="DUF7411"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95863.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="198" mass="22681" checksum="96243FEB52097E65" modified="2013-06-26" version="1">MEAYLLYSGGKDSSLAALLLKKLNYDLTLITINFGVIDSYKLAQETAKALGFRHKVIKLNREIVEKAADMVISDGYPANAINYIHKTVLETLADKYKVLADGIRRDDRVPKLSYSEIQSLEMRKGIQYISPLMGFGHKTIKELINKFFIIEEIKSGTKLTSDYEVEIREILKEKGYNPKDYFPEHKQTRVLGWKNGEQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0Z0</accession>
    <name>N6V0Z0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">DNA-directed RNA polymerase subunit K</fullName>
            <ecNumber evidence="3">2.7.7.6</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpoK</name>
        <name evidence="3" type="ORF">J422_04980</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95968.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592107.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0Z0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00493"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.976"/>
    <dbReference type="OrthoDB" id="10567at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006360">
        <property type="term" value="P:transcription by RNA polymerase I"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006366">
        <property type="term" value="P:transcription by RNA polymerase II"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042797">
        <property type="term" value="P:tRNA transcription by RNA polymerase III"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.940.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020708">
        <property type="entry name" value="DNA-dir_RNA_polK_14-18kDa_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006110">
        <property type="entry name" value="Pol_omega/Rpo6/RPB6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036161">
        <property type="entry name" value="RPB6/omega-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006111">
        <property type="entry name" value="Rpo6/Rpb6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002206">
        <property type="entry name" value="PRK01099.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002208">
        <property type="entry name" value="PRK01099.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE SUBUNIT K"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47227:SF5">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01192">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000778">
        <property type="entry name" value="RpoK/RPB6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01409">
        <property type="entry name" value="RNA_pol_Rpb6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63562">
        <property type="entry name" value="RPB6/omega subunit-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01111">
        <property type="entry name" value="RNA_POL_K_14KD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="3" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0804">Transcription</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95968.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="54" mass="6036" checksum="1654E68E76DBBACD" modified="2013-06-26" version="1">MKLTKFEIARILGARSLQISNGAYATVETNETSSLKIAYEEIKKGKVPLKPIRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6V147</accession>
    <name>N6V147_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_04710</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96013.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591983.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V147"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00961"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.921"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF5">
        <property type="entry name" value="4FE-4S FERREDOXIN-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="19"/>
            <end position="48"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="49"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96013.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="76" mass="8826" checksum="A7BF2E9329A3596D" modified="2013-06-26" version="1">MLSKIFNFLFKKKTTNEEKRIRIDYDKCKNCTACVRVCKNNVFVIKDGKVILANPQNCDMCYSCIKVCRYGALYIG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1G5</accession>
    <name>N6V1G5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methanogenesis marker protein 6</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04563</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96108.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1G5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00613"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.895"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012025">
        <property type="entry name" value="Methan_mark_6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03272">
        <property type="entry name" value="methan_mark_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09875">
        <property type="entry name" value="DUF2102"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005642">
        <property type="entry name" value="UCP005642"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96108.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="148" mass="17359" checksum="303C6F93557654C7" modified="2013-06-26" version="1">MKTKVIVLADNATTTPGKLFRYINSLNEPIIVKETCFGCYIEGEEEIVDRLAQKIRNFERNRIFCKDRGYAIWDKRRCRAFRKGGAREGFHQLEAEQKVLSYIGLALDEIDKNGVKPIEEILEKEKKILEKERKLPVEEFKRVIEKIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1I8</accession>
    <name>N6V1I8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">MobA-like NTP transferase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_03728</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96138.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591326.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1I8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00378"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.728"/>
    <dbReference type="OrthoDB" id="9782at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053669">
        <property type="entry name" value="AdoCbi-P_Guanylyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005245">
        <property type="entry name" value="CHP00454"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025877">
        <property type="entry name" value="MobA-like_NTP_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF045495">
        <property type="entry name" value="AdoCbiPCobY_Meth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00454">
        <property type="entry name" value="TIGR00454 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136:SF86">
        <property type="entry name" value="ADENOSYLCOBINAMIDE-PHOSPHATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19136">
        <property type="entry name" value="MOLYBDENUM COFACTOR GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12804">
        <property type="entry name" value="NTP_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="MobA-like NTP transferase" evidence="2">
        <location>
            <begin position="3"/>
            <end position="122"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12804"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96138.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="185" mass="21338" checksum="3737C5934CE5620F" modified="2013-06-26" version="1">MDALVMAGGKGKRLGGLEKPLIKINKKHLIDYIISALNESKIKNIYVATSPYTQRTKEYLKKHYKNIFIIETPGNGYIEDLNYCIKYFKEPFLTVCSDIINLNSKLINDIIDYYRKSDVEALNVMIPKDIYPNPHLCYENLVPAGINIVSPKKGYQKEGIYITKKLIFNINTKEDLLLAKKLFER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1X6</accession>
    <name>N6V1X6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">tRNA pseudouridine synthase D</fullName>
            <ecNumber evidence="5">5.4.99.-</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">truD</name>
        <name evidence="5" type="ORF">J422_02999</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.-" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96293.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590744.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1X6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00146"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.588"/>
    <dbReference type="OrthoDB" id="1798at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009982">
        <property type="term" value="F:pseudouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001522">
        <property type="term" value="P:pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008033">
        <property type="term" value="P:tRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.3160:FF:000001">
        <property type="entry name" value="Probable tRNA pseudouridine synthase D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1510.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020119">
        <property type="entry name" value="PsdUridine_synth_TruD_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002160">
        <property type="entry name" value="PRK00984.2-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF21">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01268">
        <property type="entry name" value="UPF0024"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRUD" evidence="4">
        <location>
            <begin position="134"/>
            <end position="343"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96293.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="394" mass="46889" checksum="38BA6BD7E2B1FE45" modified="2013-06-26" version="1">MKLRMKPEDFIVEEILDFNKIKGDKCYLYKLTKRNIESFKAFSYIAKKFKIPLKDIGYCGLKDRHAVTTQYISIPKEYGILRLDEPNLKLKLVGESKFLLLGDQEGNKFTITVRDLKEKDIGIVKENLKYLHFGAPNYFDSQRFGSVFDGKFIAKEIIKGNYEEAAKIILTKYKKSEKKIIKDLKRFIGRHWGDWDKIIKYIEEKNIKAKLYVNMINELNKSNDFKKALNYVDERLKKIFFAAYQSFLWNECIKEYLSKYIPKEDRVYYNYECGTILFYKKLDDEIFEILKNKTFPTIAPDVHYEGEEKKIIDKILKREGLKLDELYSDFGKFIYHEREVLNIPKNLKVSEFEKDELNKGKYKITLSYELNKGSYATIIIKRAFLGIKTRKRRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2R0</accession>
    <name>N6V2R0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">DUF2341 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01930</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96533.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590147.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2R0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00234"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.380"/>
    <dbReference type="OrthoDB" id="101984at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018765">
        <property type="entry name" value="DUF2341"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10102">
        <property type="entry name" value="DUF2341"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="34"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2341" evidence="2">
        <location>
            <begin position="257"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF10102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96533.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="574" mass="67598" checksum="732B0B5F33B799A5" modified="2013-06-26" version="1">MKIIRSKRGYIFTLEAVVVAFIFLSIFYLVYSIYGHNFLTNFESKKDIDLFHKSLLLKGYYIKKYSFPQEKFYTIDISKYFGNINLKTFDLCHNYSENNVIIYPEIESVNYSMLEDYKIIVTINGKNISLYSNIVNTTTLNNSLKISRIGGYAIFIYKDSAYIPIIILDSNTLNNPLKYKAFGKCGDIIYIYSNKSLKFSMKILTNDSNPFSLYSDWKYATPILIKNRDNKYLENYPVKIIFNSKEYIDEGKMDKFCRDVRFLDSNGNKLDFWIEPQSINKEHTIAWVKMNLLPNEVREIFMLYGNSNANSESDGKRVFILYDNFSNLNRSLFNVSGNVSILKSKYDNINFTYLALNNTSHIISFEKFPLEYAMKAHVYFHRKHNAYFGFYDGYNRTIVTNYEWGGDIRFENSIIDEDHYQYLYLPYELYDSWVTYEIIRDSKRSVLIVNDSQNYFLSNYIPNTVLPIVFYVDKDGWLDIDWVFVRPYNNPEPIVYNKDYDVILSINGKIFRKNANSLWKEYDDIAEELKPGLNEIKIIYSKYPLEFSFASSEENIDFKTITFSLANISVMVVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2R7</accession>
    <name>N6V2R7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01975</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96538.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2R7"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96538.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="26" mass="2921" checksum="9B6DC17E3898A50B" modified="2013-06-26" version="1">MGVCEEKDVYATLSCIELTLKELGYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2V8</accession>
    <name>N6V2V8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein uL15</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl15</name>
        <name evidence="9" type="ORF">J422_01525</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Binds to the 23S rRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 6">Belongs to the universal ribosomal protein uL15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96563.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589966.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2V8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00433"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.300"/>
    <dbReference type="OrthoDB" id="9418at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000021">
        <property type="entry name" value="50S ribosomal protein L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.990.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01341">
        <property type="entry name" value="Ribosomal_uL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027386">
        <property type="entry name" value="Rbsml_uL15_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030878">
        <property type="entry name" value="Ribosomal_uL15"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11721:SF3">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00828">
        <property type="entry name" value="Ribosomal_L27A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="5" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="8">
        <location>
            <begin position="72"/>
            <end position="141"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="1"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic residues" evidence="7">
        <location>
            <begin position="1"/>
            <end position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01341"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003888"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96563.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="143" mass="16255" checksum="4FD50ADA8946BECC" modified="2013-06-26" version="1">MIRKRRKVKKYRGSRTCGGGSHKKRRGAGNRGGRGMSGGFDHMWTWVIKYDPERFGKYGFSRHPSLVKDYETINVGELEELILKNPDKFEKEGEKYIVDLTKLGYEKLLGRGKISIPVVVKVVEVSERAREKIEAAGGEVVEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP47</accession>
    <name>N6VP47_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">THUMP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06857</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP47"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01076"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1330"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052837">
        <property type="term" value="P:thiazole biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002937">
        <property type="term" value="P:tRNA 4-thiouridine biosynthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11715">
        <property type="entry name" value="THUMP_AdoMetMT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2130.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002877">
        <property type="entry name" value="RNA_MeTrfase_FtsJ_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004114">
        <property type="entry name" value="THUMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050102">
        <property type="entry name" value="tRNA_sulfurtransferase_ThiI"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43209:SF1">
        <property type="entry name" value="TRNA SULFURTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01728">
        <property type="entry name" value="FtsJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02926">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00981">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143437">
        <property type="entry name" value="THUMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51165">
        <property type="entry name" value="THUMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="THUMP" evidence="2">
        <location>
            <begin position="15"/>
            <end position="136"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00529"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51165"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="317" mass="37348" checksum="1A1167AF85B0EC13" modified="2013-06-26" version="1">MLITTKPGFEPYLMKELKKRGYRVRYTIFRGILLLEDDLKDFNINYIFKAIPIERACSLENLKETIFNLIKEKNLKGKFVVRVNRRGRHKFTSEELEKFLGKEIVDNFNLKVDLKNYDFKINIEILQDKVYVSIFDKNFKEIVFKKNIEKLEKINRYKERPLNRSERKMSELMEKFPFIFENLNTVLDIGSAPGGWVKVLSKRAKKVYAIDRADLKVKADNIIHIKEKAENVNIDEEVDLITNDTNLYPKESMILTLKFLKNLKSGGYIIQTVKSCKENIKNDINEVINLAKDLELFKVVKLKANTKNEITLIFKKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP49</accession>
    <name>N6VP49_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0173 metal-dependent hydrolase J422_06882</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_06882</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0173 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95625.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594258.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP49"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01071"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1335"/>
    <dbReference type="OrthoDB" id="28313at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00457">
        <property type="entry name" value="UPF0173"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022877">
        <property type="entry name" value="UPF0173"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050114">
        <property type="entry name" value="UPF0173_UPF0282_UlaG_hydrolase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001911">
        <property type="entry name" value="PRK00685.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546:SF3">
        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43546">
        <property type="entry name" value="UPF0173 METAL-DEPENDENT HYDROLASE MJ1163-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12706">
        <property type="entry name" value="Lactamase_B_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="3">
        <location>
            <begin position="6"/>
            <end position="181"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00457"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95625.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="220" mass="23846" checksum="96D2375D04BCF5F1" modified="2013-06-26" version="1">MIVWYGHACFKVDNVLIDPFVPNPLCNLPFDEIMEGVDVIAVTHGHSDHLGNAETLSKSYNVPVVAIYEIACYLAERGVNAEGMNIGGSIKINGAKLTMVKAEHSSDINLNVSGGVAAGYIVNDRVYHTGDTGLFGDMELIGELYSPEVVLLPIGGRYTMGIEEALTALELIYPEVVIPMHYNTFPQIQVTEEELKAFVKGAESLGVEVIIPEIGEPLDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP92</accession>
    <name>N6VP92_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Ornithine cyclodeaminase</fullName>
            <ecNumber evidence="8">4.3.1.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Archaeal ornithine cyclodeaminase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">J422_06356</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the conversion of ornithine to proline, with the release of ammonia.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>L-ornithine = L-proline + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:24368"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:46911"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="EC" id="4.3.1.12"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AgrE/ArgZ ornithine cyclodeaminase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.3.1.12" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95690.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593514.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP92"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01302"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1237"/>
    <dbReference type="OrthoDB" id="64170at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008473">
        <property type="term" value="F:ornithine cyclodeaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="CDD" id="cd12144">
        <property type="entry name" value="SDH_N_domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.420.10">
        <property type="entry name" value="conserved putative lor/sdh protein from methanococcus maripaludis s2 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10690">
        <property type="entry name" value="putative lor/sdh protein like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005239">
        <property type="entry name" value="ArgZ/ArgE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048964">
        <property type="entry name" value="ArgZ/ArgE-like_C_1st"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048963">
        <property type="entry name" value="ArgZ/ArgE-like_C_2nd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007545">
        <property type="entry name" value="LOR/SDH_bifunc_enz_cons_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00300">
        <property type="entry name" value="TIGR00300 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21571">
        <property type="entry name" value="ArgZ-like_C_1st"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21570">
        <property type="entry name" value="ArgZ-like_C_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04455">
        <property type="entry name" value="Saccharop_dh_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0520">NAD</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="LOR/SDH bifunctional enzyme conserved" evidence="11">
        <location>
            <begin position="4"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="domain" description="Arginine dihydrolase ArgZ/ArgE-like C-terminal first subdomain" evidence="13">
        <location>
            <begin position="103"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="Arginine dihydrolase ArgZ/ArgE-like C-terminal second subdomain" evidence="12">
        <location>
            <begin position="186"/>
            <end position="403"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001911"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00052109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00056756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00061348"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00066346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00072993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00081581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04455"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF21570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF21571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN95690.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="433" mass="48462" checksum="F577A7C2CEB1F18D" modified="2013-06-26" version="1">MFMREIELRGHIIDSLILPKVLDKILDMGGDYKILRFEIGKRKTDPSYAKILVIAKDENHLDKILCELKDLGAEISEIEEVELKPAERDMVLPDNFYSTTNHKTFIRFKGKWIEVENQKMDGVIVVYPEEMRAEVKTIRQVKKGDLIVVGHKGIRVIPPEKPREESLFEFMKSEASSEKPKITIIKKIAKEMYEIRERYRKSGKGGIVVVAGPAVIHTGARDALAKLIKMGYVQVLFSGNALATHDIEFDLFGTSLGVDIRTGKSVPGGHSHHLRAINEIMKAGSIKEAVEKGVVKSGIMYECVKNNVPYVLAGSIRDDGPLPDVITDVVEAQNKMREMLKGKEMVLMLSTMLHSIATGNLLPSWVKTVCVDISPAVVTKLMDRGSSQALGVVTDVGIFLIKLVEELEKLEGEYEGEGVVKERNRDNIKVKER</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPC6</accession>
    <name>N6VPC6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">fructose-bisphosphatase</fullName>
            <ecNumber evidence="3">3.1.3.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_06234</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>beta-D-fructose 1,6-bisphosphate + H2O = beta-D-fructose 6-phosphate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:11064"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:32966"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="EC" id="3.1.3.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2 9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the inositol monophosphatase superfamily. FBPase class 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.11" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95730.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPC6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00291"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1214"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042132">
        <property type="term" value="F:fructose 1,6-bisphosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008934">
        <property type="term" value="F:inositol monophosphate 1-phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006020">
        <property type="term" value="P:inositol metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046854">
        <property type="term" value="P:phosphatidylinositol phosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.190.80:FF:000020">
        <property type="entry name" value="Fructose-1,6-bisphosphatase/inositol-1-monophosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.80">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.540.10">
        <property type="entry name" value="Fructose-1,6-Bisphosphatase, subunit A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020583">
        <property type="entry name" value="Inositol_monoP_metal-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000760">
        <property type="entry name" value="Inositol_monophosphatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020550">
        <property type="entry name" value="Inositol_monophosphatase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009321">
        <property type="entry name" value="PRK12676.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854">
        <property type="entry name" value="INOSITOL MONOPHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20854:SF4">
        <property type="entry name" value="INOSITOL-1-MONOPHOSPHATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00459">
        <property type="entry name" value="Inositol_P"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00377">
        <property type="entry name" value="IMPHPHTASES"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56655">
        <property type="entry name" value="Carbohydrate phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00629">
        <property type="entry name" value="IMP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00630">
        <property type="entry name" value="IMP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="186"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001273"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038103"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRSR" id="PIRSR600760-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95730.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="233" mass="26578" checksum="3F98890E332ED16D" modified="2013-06-26" version="1">MGKKIIKEVEKEVMPYYGKRGKVIDKNIYGDETSIFDKISEDIALKYLNDVNIISEEIGKIDNGSEWTVVIDPIDGSFNFKIGIPFFAFCFAVFKNDKPYYGLTYDFINKHIYEAYTGKGAYLNNKRIKIKEFDPNNISVCIYAENININAKRVRMLGAFGLEMCLVARGAIDACLDLRPKVRLVDIASSYIICKEAGAIIVNKKKETLNLELDHKKRYDIIVANSKDFLKLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPD4</accession>
    <name>N6VPD4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6 8">Diaminopimelate decarboxylase</fullName>
            <shortName evidence="5">DAP decarboxylase</shortName>
            <shortName evidence="5">DAPDC</shortName>
            <ecNumber evidence="5 6 8">4.1.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">lysA</name>
        <name evidence="10" type="ORF">J422_06050</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Specifically catalyzes the decarboxylation of meso-diaminopimelate (meso-DAP) to L-lysine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 8">
            <text>meso-2,6-diaminopimelate + H(+) = L-lysine + CO2</text>
            <dbReference type="Rhea" id="RHEA:15101"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:32551"/>
            <dbReference type="ChEBI" id="CHEBI:57791"/>
            <dbReference type="EC" id="4.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 5 7 8">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5 8">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; L-lysine from DL-2,6-diaminopimelate: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the Orn/Lys/Arg decarboxylase class-II family. LysA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.20" evidence="5 6 8"/>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95740.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593221.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPD4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00529"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1178"/>
    <dbReference type="OrthoDB" id="18565at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00027"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008836">
        <property type="term" value="F:diaminopimelate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06828">
        <property type="entry name" value="PLPDE_III_DapDC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.10:FF:000003">
        <property type="entry name" value="Diaminopimelate decarboxylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.10">
        <property type="entry name" value="Alanine racemase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.37.10">
        <property type="entry name" value="Lyase, Ornithine Decarboxylase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02120">
        <property type="entry name" value="LysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009006">
        <property type="entry name" value="Ala_racemase/Decarboxylase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002986">
        <property type="entry name" value="DAP_deCOOHase_LysA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022657">
        <property type="entry name" value="De-COase2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022644">
        <property type="entry name" value="De-COase2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022653">
        <property type="entry name" value="De-COase2_pyr-phos_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000183">
        <property type="entry name" value="Orn/DAP/Arg_de-COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029066">
        <property type="entry name" value="PLP-binding_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01048">
        <property type="entry name" value="lysA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43727">
        <property type="entry name" value="DIAMINOPIMELATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43727:SF2">
        <property type="entry name" value="GROUP IV DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02784">
        <property type="entry name" value="Orn_Arg_deC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01181">
        <property type="entry name" value="DAPDCRBXLASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01179">
        <property type="entry name" value="ODADCRBXLASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50621">
        <property type="entry name" value="Alanine racemase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51419">
        <property type="entry name" value="PLP-binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00878">
        <property type="entry name" value="ODR_DC_2_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00879">
        <property type="entry name" value="ODR_DC_2_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2 5 8" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="4 5 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="5 8" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="3 5 7" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Orn/DAP/Arg decarboxylase 2 N-terminal" evidence="9">
        <location>
            <begin position="49"/>
            <end position="292"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="7">
        <location>
            <position position="349"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="248"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="285"/>
            <end position="288"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="288"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="320"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="350"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="378"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5 7">
        <location>
            <position position="70"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR600183-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF02784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95740.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="425" mass="47626" checksum="BDA0DBFC49AD1701" modified="2013-06-26" version="1">MFLGNDTLTLENRMLCVDGYPFKELAEKFGTPLYVISEEQIKINYRKYIEAFKRYEKETKKNFILAYAYKANSNLAVTKLLAKLGSGADVVSGGELYIAKLSGVPSDRIVFNGNCKTEKEIIMGIEAGIRAFNVDSISELLLIEKLSKDYGEANVAFRINPDVDPKTHPKIATGLKKSKFGMNKEVAKKAIKMALKMKNINFVGLHCHIGSQITDLSPFIEATKKMMEFILELKREGIEVEDLNLGGGLGIPYYKDKKIPTIEDYANALIDTILSYDIHPNLILEPGRSIVATAGYLLGKVIHRKDMWVMIDAGMNDLIRPAIYDAYHHIENAVLKEEKEVVNVAGGLCESSDVFGREREICKPEVGDVLVIFDAGAYGISMANNYNARPKPRMIMTSKRGVFIIREREEYSDLISKDIIPPHLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPM1</accession>
    <name>N6VPM1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable S-methyl-5'-thioinosine phosphorylase</fullName>
            <ecNumber evidence="3">2.4.2.44</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">5'-methylthioinosine phosphorylase</fullName>
            <shortName evidence="3">MTI phosphorylase</shortName>
            <shortName evidence="3">MTIP</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_05613</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the reversible phosphorylation of S-methyl-5'-thioinosine (MTI) to hypoxanthine and 5-methylthioribose-1-phosphate. Involved in the breakdown of S-methyl-5'-thioadenosine (MTA), a major by-product of polyamine biosynthesis. Catabolism of (MTA) occurs via deamination to MTI and phosphorolysis to hypoxanthine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>S-methyl-5'-thioinosine + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate + hypoxanthine</text>
            <dbReference type="Rhea" id="RHEA:30643"/>
            <dbReference type="ChEBI" id="CHEBI:17368"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:48595"/>
            <dbReference type="ChEBI" id="CHEBI:58533"/>
            <dbReference type="EC" id="2.4.2.44"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3">Purine metabolism; purine nucleoside salvage.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homotrimer.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="3">Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it has been shown that conserved amino acid substitutions in the substrate binding pocket convert the substrate specificity of this enzyme from 6-aminopurines to 6-oxopurines.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.44" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95845.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592687.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPM1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00271"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1095"/>
    <dbReference type="OrthoDB" id="7681at2157"/>
    <dbReference type="UniPathway" id="UPA00606"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017061">
        <property type="term" value="F:S-methyl-5-thioadenosine phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019509">
        <property type="term" value="P:L-methionine salvage from methylthioadenosine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006166">
        <property type="term" value="P:purine ribonucleoside salvage"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd09010">
        <property type="entry name" value="MTAP_SsMTAPII_like_MTIP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1580">
        <property type="entry name" value="Nucleoside phosphorylase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01963">
        <property type="entry name" value="MTAP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010044">
        <property type="entry name" value="MTAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000845">
        <property type="entry name" value="Nucleoside_phosphorylase_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035994">
        <property type="entry name" value="Nucleoside_phosphorylase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018099">
        <property type="entry name" value="Purine_phosphorylase-2_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42679:SF2">
        <property type="entry name" value="S-METHYL-5'-THIOADENOSINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01048">
        <property type="entry name" value="PNP_UDP_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53167">
        <property type="entry name" value="Purine and uridine phosphorylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01240">
        <property type="entry name" value="PNP_MTAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="3" id="KW-0660">Purine salvage</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Nucleoside phosphorylase" evidence="4">
        <location>
            <begin position="2"/>
            <end position="231"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="8"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="44"/>
            <end position="45"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="173"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="197"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="3">
        <location>
            <position position="156"/>
        </location>
    </feature>
    <feature type="site" description="Important for substrate specificity" evidence="3">
        <location>
            <position position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95845.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="251" mass="29167" checksum="6E81D2A7E23498E6" modified="2013-06-26" version="1">MIAIIGGTGIANILRGDKEEIIETKYGKAKIMRDKDLDVILLFRHGVEHNIPPHKINYRANIYALKKLGVERILAINSVGSMKEHIKPGMFFIPIDFVEFTKKREETFYDYGKVVHIDLSEPYCPELREVLENILNKNNFEYEKGVYVCTEGPRFETKREIEIYRKFGDVVGMTGYPEVVLARELEMCYVSLCNVTNYACGLQKCLTVKEVLDILKEMEERILKVVEEFIKYNFKERNCICKDALKYAVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPN7</accession>
    <name>N6VPN7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">4-phosphopantoate--beta-alanine ligase</fullName>
            <ecNumber evidence="5">6.3.2.36</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">Phosphopantothenate synthetase</fullName>
            <shortName evidence="5">PPS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_05544</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the condensation of (R)-4-phosphopantoate and beta-alanine to 4'-phosphopantothenate in the CoA biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(R)-4-phosphopantoate + beta-alanine + ATP = (R)-4'-phosphopantothenate + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:27930"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57966"/>
            <dbReference type="ChEBI" id="CHEBI:61294"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.2.36"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal phosphopantothenate synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.36" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000042">
        <property type="protein sequence ID" value="ENN95860.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592612.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPN7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00749"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1082"/>
    <dbReference type="OrthoDB" id="10078at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016881">
        <property type="term" value="F:acid-amino acid ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.12640">
        <property type="entry name" value="Phosphopantoate/pantothenate synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02224">
        <property type="entry name" value="PPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002855">
        <property type="entry name" value="PPS/PS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038138">
        <property type="entry name" value="PPS/PS_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF041123">
        <property type="entry name" value="phpantohe_syn_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010324">
        <property type="entry name" value="PRK13761.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695">
        <property type="entry name" value="4-PHOSPHOPANTOATE--BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40695:SF1">
        <property type="entry name" value="4-PHOSPHOPANTOATE--BETA-ALANINE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02006">
        <property type="entry name" value="PPS_PS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004853">
        <property type="entry name" value="UCP004853"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 5" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="1 5" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="184"/>
            <end position="186"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="190"/>
            <end position="191"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95860.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="254" mass="28795" checksum="68BE625702161CF9" modified="2013-06-26" version="1">MIPKSHPRYESLMKREKLVDALNRGILAKAGLIAHGRGETFDYLIGEKTTETALNAIKAAAALLVLAENPIISVNGNTVALAIDEIVELAKELNGKIEINLFYRTREREIAIKNEFYRKYKDDVESGKIKILGLNAKKQIPNLDSLRGKVSEDFYNADVVLVPLEDGDRTEALVKMGKKVIAIDLNPLSRTAKAATITIVDELTRCLPLLIKYVREYKKVNRDELRKIVENFDNKKNLKETLNYIIERLKNIEF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPR7</accession>
    <name>N6VPR7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Tryptophan synthase beta chain</fullName>
            <ecNumber evidence="12">4.2.1.20</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">trpB</name>
        <name evidence="14" type="ORF">J422_05394</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 12">The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate + L-serine = D-glyceraldehyde 3-phosphate + L-tryptophan + H2O</text>
            <dbReference type="Rhea" id="RHEA:10532"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:33384"/>
            <dbReference type="ChEBI" id="CHEBI:57912"/>
            <dbReference type="ChEBI" id="CHEBI:58866"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="4.2.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 12">Tetramer of two alpha and two beta chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the TrpB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.20" evidence="12"/>
    <dbReference type="EMBL" id="APMM01000038">
        <property type="protein sequence ID" value="ENN95890.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592445.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPR7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00459"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1053"/>
    <dbReference type="OrthoDB" id="371827at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00044"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004834">
        <property type="term" value="F:tryptophan synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06446">
        <property type="entry name" value="Trp-synth_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000001">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1100:FF:000004">
        <property type="entry name" value="Tryptophan synthase beta chain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1100">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00133">
        <property type="entry name" value="Trp_synth_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006653">
        <property type="entry name" value="Trp_synth_b_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006654">
        <property type="entry name" value="Trp_synth_beta"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023026">
        <property type="entry name" value="Trp_synth_beta/beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001926">
        <property type="entry name" value="TrpB-like_PALP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036052">
        <property type="entry name" value="TrpB-like_PALP_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00263">
        <property type="entry name" value="trpB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077:SF3">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48077">
        <property type="entry name" value="TRYPTOPHAN SYNTHASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00291">
        <property type="entry name" value="PALP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001413">
        <property type="entry name" value="Trp_syn_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53686">
        <property type="entry name" value="Tryptophan synthase beta subunit-like PLP-dependent enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00168">
        <property type="entry name" value="TRP_SYNTHASE_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="9 12" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="10 12 14" id="KW-0456">Lyase</keyword>
    <keyword evidence="8 12" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Tryptophan synthase beta chain-like PALP" evidence="13">
        <location>
            <begin position="61"/>
            <end position="380"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="12">
        <location>
            <position position="94"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002786"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00049047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00291"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN95890.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="397" mass="43845" checksum="9274CA2623CDC66F" modified="2013-06-26" version="1">MGIKGLYPDKKGKYGEYGGKYVPETLMPAIEELENAFNRFWFKNEGNFKEEYYRLLKEYVGRPTPLYYAERLSEYLGCKVYLKREDLCHLGAHKINNAIGQALLAKKMGKKRVIAETGAGQHGVATAAACAKLGLECNIYMGAKDVERQKLNVFRMELMGAKVIPVYGGSQTLKDAVNEALRDWTTNVRTTYYLLGSALGPHPYPLIVREFQRVIGKEIKEQILEKEQSLPTAIVACVGGGSNAIGAFYEFLDDDVELYAVEAGGDGKRHGASLCKGDVGVLHGAKIYVLEDEYGQIEESYSISAGLDYPGVGPELSFLKDIKRIKPVFVTDYEALEGFKLLCKLEGILPALESSHAVAYALKLAENFDKDDIIVINLSGRGDKDVQTVAKALGRNL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPU8</accession>
    <name>N6VPU8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05244</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000034">
        <property type="protein sequence ID" value="ENN95925.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592267.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPU8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00301"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1024"/>
    <dbReference type="OrthoDB" id="44142at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95925.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="367" mass="44113" checksum="245E85B9CF69F935" modified="2013-06-26" version="1">MPINDEELVEIFKKLNNNVIKVNYARFSIVDIPYNVRLKLWENLKEAYKKYLKKDYIYFPDLDKEIKKYFEWTLSVIAFSFYYNNEPFPEIYKYNDSELKAVEYLLKLKPIEGYSVNDIIKAIKEKNNNEILYVLKEYVNKISYRMGELLKGIKNPILKEYIYEKWLLYKSKIDEALVYALKDPWFVEFLKCNGIEDCKKLLKNKEINFDLECYRKWFYRKIKEKLNDMIENGLSKIENKKYKIKYIDDKNKILAILEEKKPLPIKRSKILLYCVCKNNLISLKDLLNILKRLNFNNNFKKVIVVVASSVGFDERAIYVLNSEYFINNVLKDKISLILLDIKRGKVYYGLEDEYAKALDFPKLLYFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPV8</accession>
    <name>N6VPV8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PKD domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_05149</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000032">
        <property type="protein sequence ID" value="ENN95935.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592204.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000032.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPV8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00457"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1007"/>
    <dbReference type="OrthoDB" id="103676at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00146">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.10">
        <property type="entry name" value="Immunoglobulins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013783">
        <property type="entry name" value="Ig-like_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000601">
        <property type="entry name" value="PKD_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035986">
        <property type="entry name" value="PKD_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18911">
        <property type="entry name" value="PKD_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49299">
        <property type="entry name" value="PKD domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50093">
        <property type="entry name" value="PKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="517"/>
            <end position="536"/>
        </location>
    </feature>
    <feature type="domain" description="PKD" evidence="2">
        <location>
            <begin position="315"/>
            <end position="358"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50093"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95935.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="539" mass="62781" checksum="2418923277C67647" modified="2013-06-26" version="1" fragment="single">VFSSPEGYRLTPSRIYESSINISSYQKPISTLTLKDNEAILSVYSHTLIENVSGNITFKNASILNNPEPIIPYNSFNCHWNSSYIKFFIIPKEEKSGFDLLKIPINLSNNPNITMYIYINGNLSLSKIFYNINKINVTNYSGLTFYTDNLADEINLTYGHEKEILLLANNIKENITNISGYIYINRSILNVYNYGIPESSVIYYKHKLKHYNISLTDNYLYFNISFNSTNGTFGILRFYIYPKINKNISTLIYIKNLSVYKNFSKPVILPYKNLTINIIERPIKLNHPPKLKIACYIYNNKEVHFLALAYDSDNDSLKYFWDFGDNSTSTIKDPIHVYKNFSYYLVKCIVKDSLNASAKVEGILEIKNYTPVLKYCLSNITNNTIYLNISLINPFKTKILGYISVYNKNYNFYLKPNETLNLSIPINISDDVIKYNIIYYPIIKNKKNEKFEILYYTWNYERKLTSNISESTKILSYNINKTLNLNSSKIIIKINKNYLVKNYNITKSVKTFSKKSLFVYIFTTIFSFILGFILVYSNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPX7</accession>
    <name>N6VPX7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase</fullName>
            <shortName evidence="5">HPPK</shortName>
            <ecNumber evidence="5">2.7.6.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">6-hydroxymethyl-7,8-dihydropterin diphosphokinase</fullName>
            <shortName evidence="5">6-HMPDK</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin diphosphokinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="5">7,8-dihydro-6-hydroxymethylpterin pyrophosphokinase</fullName>
            <shortName evidence="5">PPPK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">mptE</name>
        <name evidence="7" type="ORF">J422_04890</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the transfer of diphosphate from ATP to 6-hydroxymethyl-7,8-dihydropterin (6-HMD), leading to 6-hydroxymethyl-7,8-dihydropterin diphosphate (6-HMDP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>6-hydroxymethyl-7,8-dihydropterin + ATP = (7,8-dihydropterin-6-yl)methyl diphosphate + AMP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11412"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44841"/>
            <dbReference type="ChEBI" id="CHEBI:72950"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.6.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; 5,6,7,8-tetrahydromethanopterin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal 6-HMPDK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.6.3" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95950.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592071.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPX7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00513"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.958"/>
    <dbReference type="UniPathway" id="UPA00065"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003848">
        <property type="term" value="F:2-amino-4-hydroxy-6-hydroxymethyldihydropteridine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004788">
        <property type="term" value="F:thiamine diphosphokinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:2001118">
        <property type="term" value="P:tetrahydromethanopterin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10240">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02131">
        <property type="entry name" value="HMPDK_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027510">
        <property type="entry name" value="HMPDK_MptE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002826">
        <property type="entry name" value="MptE-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036759">
        <property type="entry name" value="TPK_catalytic_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648">
        <property type="entry name" value="6-HYDROXYMETHYL-7,8-DIHYDROPTERIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39648:SF1">
        <property type="entry name" value="6-HYDROXYMETHYL-7,8-DIHYDROPTERIN PYROPHOSPHOKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01973">
        <property type="entry name" value="MptE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63999">
        <property type="entry name" value="Thiamin pyrophosphokinase, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 5" id="KW-0418">Kinase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="6-hydroxymethylpterin diphosphokinase MptE-like" evidence="6">
        <location>
            <begin position="39"/>
            <end position="190"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01973"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95950.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="235" mass="27286" checksum="AE85421E2270D74B" modified="2013-06-26" version="1">MYMDDREWEKIYNKIIEDFNFDKEKDVEAAKILSSFFKEKPDVKILEKMIKGKEVFIFGAGPSLKRHVKILKNIKKCQPIIVADGACKAFLEEGIIPDIIVSDLDGDIESLIECNKKGAIVVLHAHGDNIDKIKKYFHKLKNVIPTTQIPEYKKYKLYNFYGFTDGDRCCYLAYYFKAKKLILGGMDFGEYVTKYSRPEIKGEIEKADNIKAKKLRYAEYLINLLKDKIEIVFLP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ07</accession>
    <name>N6VQ07_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05015</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95975.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592122.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ07"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00486"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.983"/>
    <dbReference type="OrthoDB" id="8895at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008832">
        <property type="term" value="F:dGTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006203">
        <property type="term" value="P:dGTP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00077">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050135">
        <property type="entry name" value="dGTPase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003607">
        <property type="entry name" value="HD/PDEase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045509">
        <property type="entry name" value="HD_assoc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006675">
        <property type="entry name" value="HDIG_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00277">
        <property type="entry name" value="HDIG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11373:SF4">
        <property type="entry name" value="DEOXYNUCLEOSIDE TRIPHOSPHATE TRIPHOSPHOHYDROLASE SAMHD1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19276">
        <property type="entry name" value="HD_assoc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00471">
        <property type="entry name" value="HDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51831">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HD" evidence="1">
        <location>
            <begin position="50"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51831"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95975.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="446" mass="52651" checksum="F54E1B51A472BB33" modified="2013-06-26" version="1">MKVIRDAIHKDIYLTDEEMEVIDTEEFQRLRNIKQTGLTYLVYPSANHTRFEHSLGCLYIANKMANKIKREVDVDIETVRLSALLHDIGHPPFSHTLEIFGYNHEEVGKKIIKKLDIGNKKEVIKTLSKKNLEGHIISGEVDADRMDYLLRDSYNTGTAYGMIDIHRIITSLKTFETFGKIKIGILKKGIEAIESLLIARHQMYSAVYFHKTVRIADTMLKRAVIKEIENKNLKLDELSKMDDIALISFLRENNNYLIERLDKRRLYKRVIYYDYYELSPKEKWILVNMKEEDILNLEKELQEKFGELFIDIYPIPNLEEHDIYIIMNNHAKKLDEVSPLARSLKFSEMKLWKLAVYSDCKGINKKEFKKELFKNLDMKIHSRILDVLNKHEIVEGRKNLLELSKLSKKEFYDELYKLIFSGLIKEKFYKNKYIYSINSKNPLPIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ62</accession>
    <name>N6VQ62_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF552 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04745</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96020.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591995.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ62"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00968"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.928"/>
    <dbReference type="OrthoDB" id="59754at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.150">
        <property type="entry name" value="SepF-like protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007561">
        <property type="entry name" value="Cell_div_SepF/SepF-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038594">
        <property type="entry name" value="SepF-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04472">
        <property type="entry name" value="SepF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96020.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="135" mass="15494" checksum="7A1F424E1D5FF0BE" modified="2013-06-26" version="1">MFKKLKEIFSPEGKKIKSTKPITIEEYFESLEELPHDVVDEEKIVIKACSIYNEKDGVDAILYAESGHIVIAKIPNLEKDVDEEFIEVIKKIRDEVSKFGGKLILLGEEHLLITPKNVIIEKVIKESREKKGEEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ93</accession>
    <name>N6VQ93_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Arginine--tRNA ligase</fullName>
            <ecNumber evidence="8">6.1.1.19</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Arginyl-tRNA synthetase</fullName>
            <shortName evidence="8">ArgRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8 13" type="primary">argS</name>
        <name evidence="13" type="ORF">J422_04243</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>tRNA(Arg) + L-arginine + ATP = L-arginyl-tRNA(Arg) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:20301"/>
            <dbReference type="Rhea" id="RHEA-COMP:9658"/>
            <dbReference type="Rhea" id="RHEA-COMP:9673"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:32682"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78513"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.19"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the class-I aminoacyl-tRNA synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.19" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96050.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591728.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ93"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00674"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.830"/>
    <dbReference type="OrthoDB" id="372102at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004814">
        <property type="term" value="F:arginine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006420">
        <property type="term" value="P:arginyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00671">
        <property type="entry name" value="ArgRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1360.70">
        <property type="entry name" value="Arginyl tRNA synthetase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00123">
        <property type="entry name" value="Arg_tRNA_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001278">
        <property type="entry name" value="Arg-tRNA-ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005148">
        <property type="entry name" value="Arg-tRNA-synth_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036695">
        <property type="entry name" value="Arg-tRNA-synth_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035684">
        <property type="entry name" value="ArgRS_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008909">
        <property type="entry name" value="DALR_anticod-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00456">
        <property type="entry name" value="argS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956:SF5">
        <property type="entry name" value="ARGININE--TRNA LIGASE, CYTOPLASMIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11956">
        <property type="entry name" value="ARGINYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03485">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05746">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00750">
        <property type="entry name" value="tRNA-synt_1d"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01038">
        <property type="entry name" value="TRNASYNTHARG"/>
    </dbReference>
    <dbReference type="SMART" id="SM01016">
        <property type="entry name" value="Arg_tRNA_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00836">
        <property type="entry name" value="DALR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55190">
        <property type="entry name" value="Arginyl-tRNA synthetase (ArgRS), N-terminal 'additional' domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="4 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Arginyl tRNA synthetase N-terminal" evidence="12">
        <location>
            <begin position="1"/>
            <end position="81"/>
        </location>
    </feature>
    <feature type="domain" description="DALR anticodon binding" evidence="11">
        <location>
            <begin position="438"/>
            <end position="547"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="180"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="8">
        <location>
            <begin position="114"/>
            <end position="124"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005594"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00123"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU363038"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96050.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="547" mass="63497" checksum="287E02B571051BB8" modified="2013-06-26" version="1">MNIKEELKEAIKDLIGEDVEIVKTPNIDFGDYSTNVCFKLAKKYKKSPFEIANELVKKLENLKIKGIKDIKAVNGYINFYIDYPKLLSHLYNNTINKPLFEKKEKKIILEHTSANPNGPLHIGHLRNAVIGDSLKRILEFYGYNLETHYYVNDMGRQMALVVYGIEKFGLDESKKKDHAISEVYIKINKYLEENKDEEKKIDELMRRFEEALEKNEENEITKKFKFAVNYALEGIKETLNKLDIKHDKFVYESEYVRNGMVKEVIKKLMETGKVIKEDALYLDLSEFGIEKKMVLARANGTSLYSTRDIAYHLDKLSKCDIAIDILGADHKLTAEMVKGALKLLGSDVPEVIFYEFISLPEGSMSTRRGKFVSADEFLEEAMRIAKEECEKRGIDDINVIKTLAISAVRFYIAKFSPEKPIVFKWEDALDFEKGAVYIQYAHARCCKLLKEDGEVYEYEYTKEEKELIKLLYEFRDVIEECAKKRRVHVLANYLIELSKAFNRFYNNCPILKAEEKIRKTRLAIVKITKYVLELGLSLLGIRALEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQD7</accession>
    <name>N6VQD7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">V-type ATP synthase subunit K</fullName>
            <ecNumber evidence="10">3.6.3.14</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_04403</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the V-ATPase proteolipid subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.3.14" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96080.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591792.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQD7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00643"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.863"/>
    <dbReference type="OrthoDB" id="66135at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd18179">
        <property type="entry name" value="ATP-synt_Vo_Ao_c_NTPK_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18180">
        <property type="entry name" value="ATP-synt_Vo_Ao_c_NTPK_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.20.10">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.610">
        <property type="entry name" value="lithium bound rotor ring of v- atpase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038662">
        <property type="entry name" value="ATP_synth_F0_csu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002379">
        <property type="entry name" value="ATPase_proteolipid_c-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000245">
        <property type="entry name" value="ATPase_proteolipid_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035921">
        <property type="entry name" value="F/V-ATP_Csub_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004913">
        <property type="entry name" value="PRK06271.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10263">
        <property type="entry name" value="V-TYPE PROTON ATPASE PROTEOLIPID SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00137">
        <property type="entry name" value="ATP-synt_C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00122">
        <property type="entry name" value="VACATPASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81333">
        <property type="entry name" value="F1F0 ATP synthase subunit C"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3 8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="65"/>
            <end position="83"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="95"/>
            <end position="117"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="137"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="167"/>
            <end position="189"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="201"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="9">
        <location>
            <begin position="17"/>
            <end position="76"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="9">
        <location>
            <begin position="89"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="domain" description="V-ATPase proteolipid subunit C-like" evidence="9">
        <location>
            <begin position="163"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007296"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU363060"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96080.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="225" mass="21779" checksum="53BF52B0C1984EF1" modified="2013-06-26" version="1">MDILSQIASNPLLLGAIGAGLAVGLAGLGSGIGAGITGASGAGVIAEDPNKFGTAIVFQALPQTQGLYGFLIAILILFVFKTAPAWAMFGAGLAVGLAGLSAIGQGIASSAGLGAVAEDSGMFGKAMVFSVLPETQAIYGLLVAILLLVGVFSGAGAKEIAALGAGFAVGFAGLSGIGQGITAAGAIGATARDPDAMGKGLVLAVMPETFAIFGLLIAILIMLMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQF3</accession>
    <name>N6VQF3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Translation initiation factor 2 subunit gamma</fullName>
            <ecNumber evidence="12">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">aIF2-gamma</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">eIF-2-gamma</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="primary">eif2g</name>
        <name evidence="14" type="ORF">J422_04513</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 12">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EIF2G subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="12"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96100.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591834.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQF3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00622"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.885"/>
    <dbReference type="OrthoDB" id="7798at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001731">
        <property type="term" value="P:formation of translation preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01888">
        <property type="entry name" value="eIF2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03688">
        <property type="entry name" value="eIF2_gamma_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd15490">
        <property type="entry name" value="eIF2_gamma_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000009">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000065">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000075">
        <property type="entry name" value="Translation initiation factor 2 subunit gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00119">
        <property type="entry name" value="eIF_2_gamma"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050543">
        <property type="entry name" value="eIF2G"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015256">
        <property type="entry name" value="eIF2g_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044127">
        <property type="entry name" value="eIF2g_dom_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044128">
        <property type="entry name" value="eIF2g_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022424">
        <property type="entry name" value="TIF2_gsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03680">
        <property type="entry name" value="eif2g_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003077">
        <property type="entry name" value="PRK04000.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42854:SF3">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09173">
        <property type="entry name" value="eIF2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 12 14" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="8 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="4 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 12" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Tr-type G" evidence="13">
        <location>
            <begin position="7"/>
            <end position="201"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="19"/>
            <end position="24"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="19"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="23"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="59"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="62"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="71"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="74"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="144"/>
            <end position="147"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <begin position="179"/>
            <end position="181"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00119"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN96100.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="409" mass="44555" checksum="F63B5005EBFE5363" modified="2013-06-26" version="1">MARKSKQPEVNIGMVGHVDHGKTSLTKALTGVWTDKHSEELRRGISIRLGYADCEIRRCKNCGTYTTKKRCPNCLGETELLRRVSFVDAPGHETLMATMLSGAALMDGAILVIAANEPCPQPQTKEHLMALEILGIDKIIIVQNKIDLVDDEQAMENYEQIKEFVKGTIAEKAPIIPISAHHEANIDVLLKAIEDFIPTPKRDEKAEPRMYVARSFDINKPGTDVKDLKGGVLGGAIIQGVFKVGDEIEIRPGIKVTEENKTFWKPLTTKIVSLASGNTMLKKARPGGLIGVGTKLDPYLTKSDALSGSVVGLPGTLPPIRDKITIKVNLLDRVVGAKEELKIEPLRTGEVLMLNVGTATTAGVITSARGEIADIRLKLPICADIGDRVAISRRFGSRWRLIGYGTIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQF9</accession>
    <name>N6VQF9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_04583</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96110.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591854.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQF9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00610"/>
    <dbReference type="OrthoDB" id="24644at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03214">
        <property type="entry name" value="ABC_Iron-Siderophores_B12_Hemin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000134">
        <property type="entry name" value="Iron-enterobactin ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050153">
        <property type="entry name" value="Metal_Ion_Import_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42734:SF21">
        <property type="entry name" value="IRON ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42734">
        <property type="entry name" value="METAL TRANSPORT SYSTEM ATP-BINDING PROTEIN TM_0124-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="4">
        <location>
            <begin position="2"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="5">
        <location>
            <position position="326"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96110.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="326" mass="36806" checksum="76CBADC77AD3B6F5" modified="2013-06-26" version="1" fragment="single">MLKTENLTVGYKDYVVVDNINLEVKEGEILCIIGPNGAGKSTLLKTIASYLKPIKGVVYLEGEIIHKITPQNLAKKLAVVLTERVNPGNLTGFDIIAIGRYPYTDVFGRLTEKDKEIILECAKSVNASHLLNKYFFEMSDGEKQKIMIARALAQEPRVLLLDEPTSFLDAKHKIELTLLLRKLADEKNLAIVVTLHDIELALRVVDKIVLIKDHKIIAYGYPEEIMDNKTVNYLYDIINANYNEVIGYFELKRRKKGKIFVICGAGSGANLLRFLVKNGYNVTVGVLHKGDADYFIAKAMELNIIEEKPFKEISKEKYMEALNEIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQL1</accession>
    <name>N6VQL1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 7">Small ribosomal subunit protein eS4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps4e</name>
        <name evidence="11" type="ORF">J422_03968</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the eukaryotic ribosomal protein eS4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96180.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591406.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQL1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00423"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.778"/>
    <dbReference type="OrthoDB" id="372073at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022627">
        <property type="term" value="C:cytosolic small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd06087">
        <property type="entry name" value="KOW_RPS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00165">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.290.10:FF:000002">
        <property type="entry name" value="40S ribosomal protein S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.290.10">
        <property type="entry name" value="RNA-binding S4 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00485">
        <property type="entry name" value="Ribosomal_eS4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005824">
        <property type="entry name" value="KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000876">
        <property type="entry name" value="Ribosomal_eS4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013845">
        <property type="entry name" value="Ribosomal_eS4_central_region"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038237">
        <property type="entry name" value="Ribosomal_eS4_central_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041982">
        <property type="entry name" value="Ribosomal_eS4_KOW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013843">
        <property type="entry name" value="Ribosomal_eS4_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018199">
        <property type="entry name" value="Ribosomal_eS4_N_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002942">
        <property type="entry name" value="S4_RNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036986">
        <property type="entry name" value="S4_RNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003312">
        <property type="entry name" value="PRK04313.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581">
        <property type="entry name" value="30S/40S RIBOSOMAL PROTEIN S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11581:SF0">
        <property type="entry name" value="SMALL RIBOSOMAL SUBUNIT PROTEIN ES4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00467">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00900">
        <property type="entry name" value="Ribosomal_S4e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08071">
        <property type="entry name" value="RS4NT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01479">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002116">
        <property type="entry name" value="Ribosomal_S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00739">
        <property type="entry name" value="KOW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00363">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55174">
        <property type="entry name" value="Alpha-L RNA-binding motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00528">
        <property type="entry name" value="RIBOSOMAL_S4E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50889">
        <property type="entry name" value="S4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 7 11" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 7 8" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 8" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="RNA-binding S4" evidence="9">
        <location>
            <begin position="45"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="domain" description="KOW" evidence="10">
        <location>
            <begin position="181"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00182"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00739"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96180.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="244" mass="27780" checksum="B959357C5E11E9C2" modified="2013-06-26" version="1">MAKKGPKRHLKRLAAPVRWELPRKVHKFTVRPLPGAHPMDESLPLLLIIRDVLKYADNAREAKKIIKMGKVLVDGRVRKEEKLPVGFMDVVSLPDANENYRVLFDRKGRIKLKPTENPDVKLCKIKNKTVVKGGHIQLNLHDGRNILIRVKDPTNPVEDVYKTSDTLLITIPDQEIKAHIPFEVGKLAYITGGKHVGEIARIVDIERRGIYPDIITLETEDGEKFKTVKDYVFVIGDENPIIKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQV8</accession>
    <name>N6VQV8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">non-specific serine/threonine protein kinase</fullName>
            <ecNumber evidence="2">2.7.11.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">J422_02949</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17989"/>
            <dbReference type="Rhea" id="RHEA-COMP:9863"/>
            <dbReference type="Rhea" id="RHEA-COMP:11604"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29999"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:83421"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H(+)</text>
            <dbReference type="Rhea" id="RHEA:46608"/>
            <dbReference type="Rhea" id="RHEA-COMP:11060"/>
            <dbReference type="Rhea" id="RHEA-COMP:11605"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30013"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:61977"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.11.1"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.11.1" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96285.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590736.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQV8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00137"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.578"/>
    <dbReference type="OrthoDB" id="31344at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004674">
        <property type="term" value="F:protein serine/threonine kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd05145">
        <property type="entry name" value="RIO1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.510.10">
        <property type="entry name" value="Transferase(Phosphotransferase) domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011009">
        <property type="entry name" value="Kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051272">
        <property type="entry name" value="RIO-type_Ser/Thr_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018934">
        <property type="entry name" value="RIO_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000687">
        <property type="entry name" value="RIO_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018935">
        <property type="entry name" value="RIO_kinase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008266">
        <property type="entry name" value="Tyr_kinase_AS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45723">
        <property type="entry name" value="SERINE/THREONINE-PROTEIN KINASE RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01163">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00090">
        <property type="entry name" value="RIO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56112">
        <property type="entry name" value="Protein kinase-like (PK-like)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00109">
        <property type="entry name" value="PROTEIN_KINASE_TYR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01245">
        <property type="entry name" value="RIO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0418">Kinase</keyword>
    <keyword evidence="9" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0723">Serine/threonine-protein kinase</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="RIO kinase" evidence="12">
        <location>
            <begin position="39"/>
            <end position="266"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022527"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00048679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="SMART" id="SM00090"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96285.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="280" mass="32865" checksum="1C4791F70D533F4A" modified="2013-06-26" version="1">MSCYKKGESIDYEKYLSNKEEFELDKEYQKEILERERKFLEELKTANEVFDKRTLMILFSILAGKHLSEYIGVINSGKEAVVLKARKGHLYRAVKIYRVATCDFKTMLKYLQGDPRLHIKRSSRRAIVNAWVEKEFRNLKRASSIINAPKARLRRENVLVMDFIGVRGDPAPKLKEVEIDWENAFKTVKDFMIKLYEEELVHGDLSEYNILVKEDEIYFIDFSQSVITQHPLANALLIRDCINICNFFKKKGINCNYKDLYKEITGKEINPIDEAMIKTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR28</accession>
    <name>N6VR28_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable chemoreceptor glutamine deamidase CheD</fullName>
            <ecNumber evidence="3">3.5.1.44</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">cheD</name>
        <name evidence="4" type="ORF">J422_07097</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Probably deamidates glutamine residues to glutamate on methyl-accepting chemotaxis receptors (MCPs), playing an important role in chemotaxis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>L-glutaminyl-[protein] + H2O = L-glutamyl-[protein] + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:16441"/>
            <dbReference type="Rhea" id="RHEA-COMP:10207"/>
            <dbReference type="Rhea" id="RHEA-COMP:10208"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29973"/>
            <dbReference type="ChEBI" id="CHEBI:30011"/>
            <dbReference type="EC" id="3.5.1.44"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the CheD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.1.44" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000074">
        <property type="protein sequence ID" value="ENN95571.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594962.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR28"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01217"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1373"/>
    <dbReference type="OrthoDB" id="10499at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050568">
        <property type="term" value="F:protein-glutamine glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16352">
        <property type="entry name" value="CheD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01440">
        <property type="entry name" value="CheD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038592">
        <property type="entry name" value="CheD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005659">
        <property type="entry name" value="Chemorcpt_Glu_NH3ase_CheD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011324">
        <property type="entry name" value="Cytotoxic_necrot_fac-like_cat"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35147">
        <property type="entry name" value="CHEMORECEPTOR GLUTAMINE DEAMIDASE CHED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35147:SF1">
        <property type="entry name" value="CHEMORECEPTOR GLUTAMINE DEAMIDASE CHED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03975">
        <property type="entry name" value="CheD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64438">
        <property type="entry name" value="CNF1/YfiH-like putative cysteine hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="2 3" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0675">Receptor</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95571.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="154" mass="16591" checksum="14E9476F25F3AB62" modified="2013-06-26" version="1">MVIRVRIGGLEVARSPEVLETLLGSCVAIMLYDKGKKIGGMAHSVLPEAKNEKVTDPGKYVNTAIPALITKLAVNGARTNKLIAKLAGGANMFKTSKNSINVGEKNVETAKRLLKQYNIPLVGEDTGGNRSRIVRFYLKTGDVEVRKGGETIII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR40</accession>
    <name>N6VR40_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Rhodanese domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_07027</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000068">
        <property type="protein sequence ID" value="ENN95586.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594741.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR40"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00919"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1361"/>
    <dbReference type="OrthoDB" id="135517at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043828">
        <property type="term" value="F:tRNA 2-selenouridine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030818">
        <property type="entry name" value="Arch_SelU_Nterm"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017582">
        <property type="entry name" value="SelU"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04568">
        <property type="entry name" value="arch_SelU_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30401">
        <property type="entry name" value="TRNA 2-SELENOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30401:SF0">
        <property type="entry name" value="TRNA 2-SELENOURIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Rhodanese" evidence="1">
        <location>
            <begin position="98"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95586.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="219" mass="24703" checksum="F1757F3447818DA9" modified="2013-06-26" version="1">MDEDILARLITFDKDVVLAIVLDSGKKMITDGEKILAGKIGGDLASFIIKEAKHIKSPTVKEFNNHKIYFEKINIDKFLGSLDKSFLRDVINVSELMTLKNYILVDVRSPREFKEGTIEGAINIPLFLDDEHELIGKTYKEMGKDKAIDLAINIVEKGLKRILNEAKKLDRDKTIVVFCARGGLRSQVMALIFKLLGFKVKRLLGGYKAYKLSSIKKLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR61</accession>
    <name>N6VR61_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96395.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR61"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00789"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96395.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="50" mass="6024" checksum="182CD2C6AE0A51D8" modified="2013-06-26" version="1" fragment="single">KKLCKVYDKRFEYGCLTVLEGILAGVFPKDCPYVKNLKNYEEPRFYRFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR63</accession>
    <name>N6VR63_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Sulfopyruvate decarboxylase subunit alpha</fullName>
            <ecNumber evidence="7">4.1.1.79</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_06862</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Involved in the biosynthesis of the coenzyme M (2-mercaptoethanesulfonic acid). Catalyzes the decarboxylation of sulfopyruvate to sulfoacetaldehyde.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>3-sulfopyruvate + H(+) = sulfoacetaldehyde + CO2</text>
            <dbReference type="Rhea" id="RHEA:20948"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:57940"/>
            <dbReference type="ChEBI" id="CHEBI:58246"/>
            <dbReference type="EC" id="4.1.1.79"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; coenzyme M biosynthesis; sulfoacetaldehyde from phosphoenolpyruvate and sulfite: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Heterododecamer composed of 6 subunits alpha and 6 subunits beta.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the ComD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.79" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95621.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594250.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR63"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01075"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1331"/>
    <dbReference type="OrthoDB" id="53192at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050545">
        <property type="term" value="F:sulfopyruvate decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030976">
        <property type="term" value="F:thiamine pyrophosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019295">
        <property type="term" value="P:coenzyme M biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07035">
        <property type="entry name" value="TPP_PYR_POX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000039">
        <property type="entry name" value="Indolepyruvate oxidoreductase subunit IorA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022502">
        <property type="entry name" value="Sulfopyruvate_deCO2ase_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012001">
        <property type="entry name" value="Thiamin_PyroP_enz_TPP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051818">
        <property type="entry name" value="TPP_dependent_decarboxylase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03845">
        <property type="entry name" value="sulfopyru_alph"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42818:SF1">
        <property type="entry name" value="SULFOPYRUVATE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42818">
        <property type="entry name" value="SULFOPYRUVATE DECARBOXYLASE SUBUNIT ALPHA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02776">
        <property type="entry name" value="TPP_enzyme_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0174">Coenzyme M biosynthesis</keyword>
    <keyword evidence="2" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="12" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine pyrophosphate enzyme N-terminal TPP-binding" evidence="11">
        <location>
            <begin position="1"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022545"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00037396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00037914"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038875"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00060971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00068303"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02776"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95621.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="167" mass="18957" checksum="B8DAAFAA5725A1FF" modified="2013-06-26" version="1">MKGSLAIYRALKDSGIKFICSVPCANLKNLLKLVDEDEYFLHVPATREEEAFGICAGAHMAGKKTAILMQNSGLGNSINAIASLYKIYQIPTLLIISHRGELKEKIPAQIPMGRWVTKLLDVCEIPYYKPKTPEKAYKIIKYASEYMEKISYPVAVLFDALYWEWDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRA6</accession>
    <name>N6VRA6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Phenylalanine--tRNA ligase beta subunit</fullName>
            <ecNumber evidence="12">6.1.1.20</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Phenylalanyl-tRNA synthetase beta subunit</fullName>
            <shortName evidence="12">PheRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12 14" type="primary">pheT</name>
        <name evidence="14" type="ORF">J422_02380</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>tRNA(Phe) + L-phenylalanine + ATP = L-phenylalanyl-tRNA(Phe) + AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19413"/>
            <dbReference type="Rhea" id="RHEA-COMP:9668"/>
            <dbReference type="Rhea" id="RHEA-COMP:9699"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58095"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78531"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.20"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="12">Tetramer of two alpha and two beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 12">Belongs to the phenylalanyl-tRNA synthetase beta subunit family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.20" evidence="12"/>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96435.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590348.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRA6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00357"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.468"/>
    <dbReference type="OrthoDB" id="10073at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009328">
        <property type="term" value="C:phenylalanine-tRNA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004826">
        <property type="term" value="F:phenylalanine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006432">
        <property type="term" value="P:phenylalanyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00769">
        <property type="entry name" value="PheRS_beta_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.50.40.10:FF:000003">
        <property type="entry name" value="Phenylalanine--tRNA ligase beta subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.56.10">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.40.10">
        <property type="entry name" value="Phenylalanyl-trna Synthetase, Chain B, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00284">
        <property type="entry name" value="Phe_tRNA_synth_beta2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005146">
        <property type="entry name" value="B3/B4_tRNA-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009061">
        <property type="entry name" value="DNA-bd_dom_put_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045060">
        <property type="entry name" value="Phe-tRNA-ligase_IIc_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004531">
        <property type="entry name" value="Phe-tRNA-synth_IIc_bsu_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020825">
        <property type="entry name" value="Phe-tRNA_synthase-like_B3/B4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022918">
        <property type="entry name" value="Phe_tRNA_ligase_beta2_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041616">
        <property type="entry name" value="PheRS_beta_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040659">
        <property type="entry name" value="PhetRS_B1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005147">
        <property type="entry name" value="tRNA_synthase_B5-dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00471">
        <property type="entry name" value="pheT_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947:SF0">
        <property type="entry name" value="PHENYLALANINE--TRNA LIGASE BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10947">
        <property type="entry name" value="PHENYLALANYL-TRNA SYNTHETASE BETA CHAIN AND LEUCINE-RICH REPEAT-CONTAINING PROTEIN 47"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03483">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03484">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18262">
        <property type="entry name" value="PhetRS_B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17759">
        <property type="entry name" value="tRNA_synthFbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00873">
        <property type="entry name" value="B3_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00874">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46955">
        <property type="entry name" value="Putative DNA-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51483">
        <property type="entry name" value="B5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="8 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 12 14" id="KW-0436">Ligase</keyword>
    <keyword evidence="9 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 12" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="B5" evidence="13">
        <location>
            <begin position="271"/>
            <end position="346"/>
        </location>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="324"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="330"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="333"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="12">
        <location>
            <position position="334"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <note>shared with alpha subunit</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51483"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN96435.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="540" mass="62347" checksum="6AEDFAC17C8B1C77" modified="2013-06-26" version="1">MPTVNVKKYDLERLVNMPLDDKFIEDKFPMMGVEVEEIREEEGEKLISFSVNPNRPDYLSVEGLARGFRGFIGIDPGMSKYDVEKSDVKLYVENVKTRPYIAMALIKGIVIDNYVLESIINLQEKLHWVIGRDRKKVAIGIHDFDKVKPPFYYKEVDNISFVPLGYNEEMSAEEILEKHEKGIKYRHLIKGKYPIILDSEGNVLSFPPIINSELTRVTERTRNLLIDVTGTDKVAVEKTLNILVTALAERKYSKIHSVEVIGKEREIYPKLKEETIVVNPDYINKILGTKLQPGIMINYLRRAKLDAVFSENKLIVYIPPYRVDIFSEVDIAEEIAIAYGYDKFKGVYPEIATIGELDKLEKRCDFIREIMIGLGFYEVINLILTNEKVLFEKMKLKGDYIEVLKPASIEHRILRTSILPLIMETLKINEHKELPIKIFEIGDCVILDGNRVREIKKIAGAIMSNETNYSEIKSHVEALLRELRIDYEIDRYDHPSFIKGRCAKIIKDKRVIGYFGEIDPEVLTNFDLEFPVSAFELLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRC5</accession>
    <name>N6VRC5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Type 2 DNA topoisomerase 6 subunit B</fullName>
            <ecNumber evidence="6">5.6.2.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Type II DNA topoisomerase VI subunit B</fullName>
            <shortName evidence="6">TopoVI-B</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">top6B</name>
        <name evidence="9" type="ORF">J422_02135</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Relaxes both positive and negative superturns and exhibits a strong decatenase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>ATP-dependent breakage, passage and rejoining of double-stranded DNA.</text>
            <dbReference type="EC" id="5.6.2.2"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer. Heterotetramer of two Top6A and two Top6B chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the TOP6B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.6.2.2" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96450.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590227.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRC5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01176"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.420"/>
    <dbReference type="OrthoDB" id="65493at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003918">
        <property type="term" value="F:DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00823">
        <property type="entry name" value="TopoIIB_Trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.230.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00322">
        <property type="entry name" value="Top6B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020568">
        <property type="entry name" value="Ribosomal_Su5_D2-typ_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014721">
        <property type="entry name" value="Ribsml_uS5_D2-typ_fold_subgr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005734">
        <property type="entry name" value="TopoVI_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015320">
        <property type="entry name" value="TopoVI_B_transducer"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003218">
        <property type="entry name" value="PRK04184.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011438">
        <property type="entry name" value="PRK14867.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01052">
        <property type="entry name" value="top6b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48444:SF1">
        <property type="entry name" value="DNA TOPOISOMERASE 6 SUBUNIT B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09239">
        <property type="entry name" value="Topo-VIb_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006553">
        <property type="entry name" value="TopoVI_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54211">
        <property type="entry name" value="Ribosomal protein S5 domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 6 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="1 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0799">Topoisomerase</keyword>
    <feature type="domain" description="Histidine kinase/HSP90-like ATPase" evidence="8">
        <location>
            <begin position="29"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="7">
        <location>
            <begin position="600"/>
            <end position="655"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="97"/>
            <end position="98"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="107"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="575"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023029"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00387"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96450.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="656" mass="75713" checksum="069788920A81BAA7" modified="2013-06-26" version="1">MSTDIFKEFKEHSVAEFFRKNKHMLGYSGKIRSLTTVVHELVTNSLDACEEAGILPEIKVEIEKLGSDHYRVAVEDNGPGIPLEFIPKVFGKMLAGSKMHRFIQSRGQQGIGAAGVLLFSQITTGKPLKIKTSLGDGKIYEVEVKMNVEKNEGEIVSKKVKKGNWRGTRVEGEFKEVAYNRGELSAYEYLRRISLATPHARIILEDPYGKVVFDRVVKEMPKKPEEMKPHPHGLTVDELLYIARKTKAKKVSSMLVSELSRFSQKRIDELKQYMLRDLLLEKFKNTIYWKNVIKCYLNIDIEKYLKKFSDYLSNEEIEFVNVLINSLPESLDDLKRYALKYIYMEYLFKKLSKDEIENNKNQFKKIPENFIEWAEKNYLSTTHVNELNKKVREIVKTPKEFIELLEKRRLISKEELNKFKEKVEEILDKNPKNLSWDEAELIVNCLQNMEFMAPPTSGLRPIGAENIEKSLKDLLKPDFVKAITRNPKTYKGIPFIVEAAIAYGGNAGKDSSEGKKMEIMRFANHVPLLYDASACGITKAVKSINWRRYGIKEEAPITVFVNLISTFIPYTSAGKQAIACSENENEEIYNEIRYALMICARALEKYLSKIRREAEEEKKRKYVLKYAKIFAEALANILNKNQEEIEKKVVELLSKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRI0</accession>
    <name>N6VRI0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 6">2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate synthase</fullName>
            <shortName evidence="5">ADH synthase</shortName>
            <shortName evidence="5">ADHS</shortName>
            <shortName evidence="5">ADTH synthase</shortName>
            <ecNumber evidence="5 6">2.2.1.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">aroA'</name>
        <name evidence="8" type="ORF">J422_06010</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes a transaldol reaction between 6-deoxy-5-ketofructose 1-phosphate (DKFP) and L-aspartate semialdehyde (ASA) with an elimination of hydroxypyruvaldehyde phosphate to yield 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonate (ADH). Plays a key role in an alternative pathway of the biosynthesis of 3-dehydroquinate (DHQ), which is involved in the canonical pathway for the biosynthesis of aromatic amino acids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate + L-aspartate 4-semialdehyde = 2,3-dioxopropyl phosphate + 2-amino-2,3,7-trideoxy-D-lyxo-hept-6-ulosonate</text>
            <dbReference type="Rhea" id="RHEA:25952"/>
            <dbReference type="ChEBI" id="CHEBI:58859"/>
            <dbReference type="ChEBI" id="CHEBI:58860"/>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="2.2.1.10"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodecamer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the DeoC/FbaB aldolase family. ADHS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.10" evidence="5 6"/>
    <dbReference type="EMBL" id="APMM01000048">
        <property type="protein sequence ID" value="ENN95766.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593137.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRI0"/>
    <dbReference type="SMR" id="N6VRI0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00922"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1170"/>
    <dbReference type="OrthoDB" id="50091at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004332">
        <property type="term" value="F:fructose-bisphosphate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016836">
        <property type="term" value="F:hydro-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016744">
        <property type="term" value="F:transketolase or transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00958">
        <property type="entry name" value="DhnA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00960">
        <property type="entry name" value="ADH_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010210">
        <property type="entry name" value="ADH_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002915">
        <property type="entry name" value="DeoC/FbaB/LacD_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050456">
        <property type="entry name" value="DeoC/FbaB_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041720">
        <property type="entry name" value="FbaB-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01949">
        <property type="entry name" value="ADH_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005556">
        <property type="entry name" value="PRK07226.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916:SF1">
        <property type="entry name" value="3-HYDROXY-5-PHOSPHONOOXYPENTANE-2,4-DIONE THIOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47916">
        <property type="entry name" value="FRUCTOSE-BISPHOSPHATE ALDOLASE CLASS 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01791">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF038992">
        <property type="entry name" value="Aldolase_Ia"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01133">
        <property type="entry name" value="DeoC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0704">Schiff base</keyword>
    <keyword evidence="2 5" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Proton acceptor" evidence="5">
        <location>
            <position position="33"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="5 7">
        <location>
            <position position="153"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with dihydroxyacetone-P" evidence="7">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="5">
        <location>
            <position position="184"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="33"/>
            <end position="37"/>
        </location>
        <ligand>
            <name>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="153"/>
            <end position="155"/>
        </location>
        <ligand>
            <name>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="209"/>
            <end position="210"/>
        </location>
        <ligand>
            <name>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="237"/>
            <end position="238"/>
        </location>
        <ligand>
            <name>1-deoxy-D-threo-hexo-2,5-diulose 6-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58861"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00960"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR01949"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR038992-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95766.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="272" mass="29659" checksum="A911FB333D6C0C26" modified="2013-06-26" version="1">MKLFDNIKNIGKLVRLERIFNRETEKTVIVPMDHGVSNGPIKGLINMKETINNVAEGGANAVLLHKGIVRHGHRGYGKDIGLIIHLSAGTSISPNPLKKVIVTTVEEAIRMGADAVSVHVNVGSEEDWEAYRDLGLIAETCEYWGMPLIAMMYPRGKHIKNERDPELVAHAARLGAELGADIIKTSYTGDVDSFREVVKGCPAPIVVAGGPKTRTDEEFLQMIKDAMEAGAVGVAVGRNIFQHDDVVGITRAVCKIVHENADVDEALKEIRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRR0</accession>
    <name>N6VRR0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Cell division ATPase MinD</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_05618</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95846.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592689.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRR0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00270"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1096"/>
    <dbReference type="OrthoDB" id="31168at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000285">
        <property type="entry name" value="Sporulation initiation inhibitor Soj"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010224">
        <property type="entry name" value="MinD_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025501">
        <property type="entry name" value="MinD_FleN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01969">
        <property type="entry name" value="minD_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF10">
        <property type="entry name" value="ATPASE INVOLVED IN CHROMOSOME PARTITIONING, PARA_MIND FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003092">
        <property type="entry name" value="MinD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="5" id="KW-0132">Cell division</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="4">
        <location>
            <begin position="2"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="9"/>
            <end position="16"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRSR" id="PIRSR003092-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95846.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="255" mass="27760" checksum="8BDE0507164DA7B6" modified="2013-06-26" version="1">MIITVASGKGGVGKTTCSASLAVALAKLNKKVLVVDGDISMANLGILFDMEKKKPSLHEVLSGEADVREAIYKHKTGVFVLPTSLSLDGYRKADIDLFSDAILDVADEYDYVIIDAPAGLNREMAIHLAIADKLLLVITPEMFSIVDAARLKESSEMAGTPLMGIVLNRVGRDFGELGKDEIESLIKGKVLVEIPEDENIRNAALNKKTVIEYKPKSPASLAFMKLASIIAGEPIRIEIKENLGIIERIKRLFIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRT4</accession>
    <name>N6VRT4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type 11 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05509</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000041">
        <property type="protein sequence ID" value="ENN95876.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592567.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRT4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00001"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1076"/>
    <dbReference type="OrthoDB" id="1018at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050508">
        <property type="entry name" value="Methyltransf_Superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42912:SF80">
        <property type="entry name" value="METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="38"/>
            <end position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95876.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="190" mass="22155" checksum="D88C26CDB64E4931" modified="2013-06-26" version="1">MNFDKYYKEYDEWFEKNKEIYLKELNALKKYIPKGKGLEVGVGTGRFAQPLGIKYGLDPSKNMAKLAEERGVKVYIGYAENMPFEDNSFDFILLNTTLCFLKDVEKALKEIKRVLKPGGYLIIGMIDKNSKLGKIYEAKKDKSKFYKDANFFSLDEVIELLKRFGFKDIEYERVRLSEEDDGSYVIIRAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRX7</accession>
    <name>N6VRX7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A)</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Cyclic oligoadenylate synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="15" type="ORF">J422_05279</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CRISPR-associated Cas10/Csm1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000035">
        <property type="protein sequence ID" value="ENN95916.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592280.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRX7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00743"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1031"/>
    <dbReference type="OrthoDB" id="57429at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004527">
        <property type="term" value="F:exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd09680">
        <property type="entry name" value="Cas10_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3210.10">
        <property type="entry name" value="Hypothetical protein af1432"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054767">
        <property type="entry name" value="Cas10-Cmr2_palm2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013408">
        <property type="entry name" value="Cas10/Csm1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052117">
        <property type="entry name" value="Cas10/Csm1_subtype-III-A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041062">
        <property type="entry name" value="Csm1_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000160">
        <property type="entry name" value="GGDEF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006674">
        <property type="entry name" value="HD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043128">
        <property type="entry name" value="Rev_trsase/Diguanyl_cyclase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02578">
        <property type="entry name" value="cas_TM1811_Csm1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36528">
        <property type="entry name" value="CRISPR SYSTEM SINGLE-STRAND-SPECIFIC DEOXYRIBONUCLEASE CAS10/CSM1 (SUBTYPE III-A)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36528:SF1">
        <property type="entry name" value="CRISPR SYSTEM SINGLE-STRAND-SPECIFIC DEOXYRIBONUCLEASE CAS10_CSM1 (SUBTYPE III-A)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22335">
        <property type="entry name" value="Cas10-Cmr2_palm2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18211">
        <property type="entry name" value="Csm1_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01966">
        <property type="entry name" value="HD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109604">
        <property type="entry name" value="HD-domain/PDEase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50887">
        <property type="entry name" value="GGDEF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="13" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9" id="KW-0269">Exonuclease</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5" id="KW-0540">Nuclease</keyword>
    <keyword evidence="6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="GGDEF" evidence="14">
        <location>
            <begin position="535"/>
            <end position="700"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="13">
        <location>
            <begin position="665"/>
            <end position="692"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00032922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN95916.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="853" mass="100877" checksum="94EA3857D2DF070D" modified="2013-06-26" version="1">MNYYRAVVIGALLHDIGKFVQRKIMDEKNIKKPPKHDIEGYNYLKKLFDDGFLSFLSNEEKKIILDIVKEHHNNKIKDGVIGIVRLADWLSSGERKTVEESEVLKGYGKDIKLLSIFETANVLNYIKSEIILENLYKIGYKYPLNELKLDKNVIFTTKPHPNDSYLNLFSDFETALRDFKNKNDVSFEELLQLLYKYTWCIPSATFWEREKIKGSYPDISLYDHLKTTCAIAACLYRLYKNEKINDKKLKEYLERKNWEDKIFCLIHGDISGIQDFIFTVKNKYAAKTLRGRSFYLDFLMEYLARYICKELDLPIANILFCGGGHFYILSYKVDDGKIEEFEKKINDILFKMFKTKVYVYLVKEDVSLNDFLNFSDVWKRVSDRTVVRKLRRFDYKLEEIFDYIDEGKPERCEICGIELDEDKKREDPEIEGYICKYCKSFIELTKILKNSNKFSLKEIENIEVIKDLKRGLNVKNSKEKEFFRYYLDEYNLPDDNGELIIPFKIFPISFPLDESGKILDLNKLAEKAEERTGTKKIAVLKMDVDNLGELFTKGLKIIKNNKDEKSKNLDSISRMSTLSNFLSLFFTGYIPYLIKTGKFKDKEGKEHEFKDNIYLIYAGGDDTLITGSWDAVWELAKKIREDFKEFVCYNPFITLSAGIYLTSPKFKFKRAVEFAEEELERAKDNEINSIKKNSLSIFSTSLNWDLEVKYIPRFWEKLKDIERELKLENELINEMLSLEKYCEKFNEDILEDKFNELIKDTNKKRILHVSQVVAEMLNKIVKKGENGETILYLPYYWRLLYYIKRNYKNNYEKVKFLEDYIKGKVHQMLLQNIDLSLNDLKVAAKITELKWRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRY9</accession>
    <name>N6VRY9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Thiamine-monophosphate kinase</fullName>
            <shortName evidence="1">TMP kinase</shortName>
            <shortName evidence="1">Thiamine-phosphate kinase</shortName>
            <ecNumber evidence="1">2.7.4.16</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">thiL</name>
        <name evidence="3" type="ORF">J422_05249</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Catalyzes the ATP-dependent phosphorylation of thiamine-monophosphate (TMP) to form thiamine-pyrophosphate (TPP), the active form of vitamin B1.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>thiamine phosphate + ATP = thiamine diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:15913"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:37575"/>
            <dbReference type="ChEBI" id="CHEBI:58937"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine diphosphate from thiamine phosphate: step 1/1.</text>
    </comment>
    <comment type="miscellaneous">
        <text evidence="1">Reaction mechanism of ThiL seems to utilize a direct, inline transfer of the gamma-phosphate of ATP to TMP rather than a phosphorylated enzyme intermediate.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the thiamine-monophosphate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.16" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000034">
        <property type="protein sequence ID" value="ENN95926.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592268.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRY9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00300"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1025"/>
    <dbReference type="OrthoDB" id="45909at2157"/>
    <dbReference type="UniPathway" id="UPA00060">
        <property type="reaction ID" value="UER00142"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009229">
        <property type="term" value="P:thiamine diphosphate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02194">
        <property type="entry name" value="ThiL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02128">
        <property type="entry name" value="TMP_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270:SF3">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005303">
        <property type="entry name" value="Thiam_monoph_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0418">Kinase</keyword>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0784">Thiamine biosynthesis</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="2">
        <location>
            <begin position="27"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="28"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="51"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>4</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <begin position="117"/>
            <end position="118"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="212"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>3</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="214"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="215"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>5</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="305"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02128"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95926.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="309" mass="35269" checksum="CCEF4EB44D3AA50B" modified="2013-06-26" version="1">MDEIKVIEIAKSLLNFKRDYVVKGIDDDGAVLKIGKKYIILTSDMMLRETHIPEILSAYEIGARILTANVSDLLAMGAEPLAFLLSLAVDRDEKFIYELYRGLSDYSKHYNCPIVGGDTVKGDLILSGFAIGITNKPLFREGKINDDIAVTNDIGRVYCSIYLYNLYKKGKISYKEFLNYAEKYKNIMEKLRKPVARMEILDVKEFLHGACDISDGLAKEIRYFKNFEIYGDKLLKSIPEDVLSFCDEFNLDPIKVALNSGEEFEILFTTDKLNKVKKHVKVNKIGKIIENGQYIDGKELKDIGYVHKW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSE7</accession>
    <name>N6VSE7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Precorrin-8X methylmutase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cbiC</name>
        <name evidence="6" type="ORF">J422_04323</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the CobH/CbiC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96066.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591761.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSE7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00659"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.847"/>
    <dbReference type="OrthoDB" id="24491at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016993">
        <property type="term" value="F:precorrin-8X methylmutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10230">
        <property type="entry name" value="Cobalamin biosynthesis CobH/CbiC, precorrin-8X methylmutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003722">
        <property type="entry name" value="Cbl_synth_CobH/CbiC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036588">
        <property type="entry name" value="CobH/CbiC_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004901">
        <property type="entry name" value="PRK06264.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43588">
        <property type="entry name" value="COBALT-PRECORRIN-8 METHYLMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43588:SF1">
        <property type="entry name" value="COBALT-PRECORRIN-8 METHYLMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02570">
        <property type="entry name" value="CbiC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF63965">
        <property type="entry name" value="Precorrin-8X methylmutase CbiC/CobH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cobalamin biosynthesis precorrin-8X methylmutase CobH/CbiC" evidence="5">
        <location>
            <begin position="10"/>
            <end position="201"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009774"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF02570"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96066.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="208" mass="22532" checksum="A726C0979B3AC10E" modified="2013-06-26" version="1">MGAISKDGLEIAKRAREIVREKIKDVLGEKIKNFSKEELLIIERVVHATADPEYAKLLVFKNNPIYHGVKAIKEGKPIITDVNMVKAGIRYDKTYCFIDDERAYELAKQGITRAVASMRLAKEVIDGGIVVIGNAPTALLELINLINSGIKPKLVIAVPVGFVKASEAKEMIRNTDVPSISTIGPKGGSSVAVAIANGIIALSKNERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSF5</accession>
    <name>N6VSF5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Acetyl-CoA decarbonylase/synthase complex subunit delta</fullName>
            <shortName evidence="1">ACDS complex subunit delta</shortName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Corrinoid/iron-sulfur component small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">cdhD</name>
        <name evidence="3" type="ORF">J422_04383</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible cleavage of acetyl-CoA, allowing autotrophic growth from CO(2). Probably maintains the overall quaternary structure of the ACDS complex.</text>
    </comment>
    <comment type="subunit">
        <text evidence="1">Heterodimer of delta and gamma chains. The ACDS complex is made up of alpha, epsilon, beta, gamma and delta chains with a probable stoichiometry of (alpha(2)epsilon(2))(4)-beta(8)-(gamma(1)delta(1))(8).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the CdhD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96076.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSF5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00647"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.859"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.20">
        <property type="entry name" value="Dihydropteroate synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01135">
        <property type="entry name" value="CdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016041">
        <property type="entry name" value="Ac-CoA_synth_d_su_TIM-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051069">
        <property type="entry name" value="ACDS_complex_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004486">
        <property type="entry name" value="CO_DH/Ac-CoA_synth_dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011005">
        <property type="entry name" value="Dihydropteroate_synth-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00381">
        <property type="entry name" value="cdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003378">
        <property type="entry name" value="PRK04452.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36214">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36214:SF5">
        <property type="entry name" value="ACETYL-COA DECARBONYLASE_SYNTHASE COMPLEX SUBUNIT DELTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03599">
        <property type="entry name" value="CdhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51717">
        <property type="entry name" value="Dihydropteroate synthetase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CO dehydrogenase/acetyl-CoA synthase delta subunit TIM barrel" evidence="2">
        <location>
            <begin position="75"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF03599"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96076.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="387" mass="43487" checksum="2A1BEDB1F2C929BD" modified="2013-06-26" version="1">MLKDIIKILEKVGKIEIENVKITAEEIIINIPQSPPITIPQTPAIKQSLAEEGLIEPPEVDFELPIEKYSGRIREVQFGKPKSEGGRGKVVKIGGQTSLYRFEAPYPNPPAITFDIFDMPMPGLPKPIREFFEDVMEDPCDWARKCVKEFGADMITIHHISTDPKIKDKSPKEAAKLMEDLMQAVDVPFVIGGSGNPEKDPHVLAACAEVCEGDRCLLASANLELDYRKIVEAAMKYDHNVLAWSIMDPNMAKELNRKLIEAGLDPNRIVMDPTTCALGYGIEFSINAITRLRIEGLKGNELCNMPMSSGTTNAIGAREAWMKNPEWGDRRYRLPLWEITTGITMLMSGVDLFMMLNPIAVRILKDIGRKLTTKPKKIMADYDWIYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSF7</accession>
    <name>N6VSF7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Thioredoxin reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_00135</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96810.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSF7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01141"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.27"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004791">
        <property type="term" value="F:thioredoxin-disulfide reductase (NADPH) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019430">
        <property type="term" value="P:removal of superoxide radicals"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.50.60">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036188">
        <property type="entry name" value="FAD/NAD-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023753">
        <property type="entry name" value="FAD/NAD-binding_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050097">
        <property type="entry name" value="Ferredoxin-NADP_redctase_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005982">
        <property type="entry name" value="Thioredox_Rdtase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01292">
        <property type="entry name" value="TRX_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48105">
        <property type="entry name" value="THIOREDOXIN REDUCTASE 1-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07992">
        <property type="entry name" value="Pyr_redox_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00368">
        <property type="entry name" value="FADPNR"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00469">
        <property type="entry name" value="PNDRDTASEII"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51905">
        <property type="entry name" value="FAD/NAD(P)-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0472">Membrane</keyword>
    <keyword evidence="2" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="3">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="FAD/NAD(P)-binding" evidence="4">
        <location>
            <begin position="9"/>
            <end position="292"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF07992"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96810.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="307" mass="33966" checksum="0126573A1F32F2FF" modified="2013-06-26" version="1">MLLKGVIMYDVIIIGAGPAGLSAGVYAMRGKLKAICIEKENPGGRIAEAGIVENYLGFEEIRGYELAERFRKHAEKFKLPIIYDEVVKIEKENGYFKVITKKSQYITKKIVIATGTRAKKLNINEDKFIGKGVSYCTMCDAFFYLNKDVIVLGRDTPAIMSAINLKDIAKRVILITDKKELKAAEPIMFDRLKEAKNIEIIYNAEILEIVGDERAEGVKIRVNGEEKIIKADGIFISYGHVPNTDFLKGVVELDKNGFVITDENCKTSVNGIYAVGDVRGGVMQVAKAVGDGCIAMANIIKELQRGM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSI0</accession>
    <name>N6VSI0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00245</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96830.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589736.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSI0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01120"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.49"/>
    <dbReference type="OrthoDB" id="227825at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007381">
        <property type="entry name" value="CheF1/F2"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42201">
        <property type="entry name" value="TAXIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42201:SF1">
        <property type="entry name" value="TAXIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04283">
        <property type="entry name" value="CheF-arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96830.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="241" mass="29057" checksum="2C392A69638E21B2" modified="2013-06-26" version="1">MSYVAKFSGRGVVITKHHRKLYSFLYKKWEPLDITLYRDKIVFDFGDNKIELSLDNIIDIGERIPEEIIKTSKYCLEGIEYYSTVTTYINFEKTVIAFATESMIYEKTLIISFLRKLFQILLNNKDVMVRYNIKENFYWEKGKIKFVDKKIRDKGIMRVEHYITVETKNDRYYLFTNIKDLKISEDKIKDRVLPVLEIVQKMWIKNISSYFYSDDWKVKLFILRYLREIMGLDIGILKYLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSJ4</accession>
    <name>N6VSJ4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Phosphoribosyl-ATP pyrophosphatase</fullName>
            <shortName evidence="9">PRA-PH</shortName>
            <ecNumber evidence="9">3.6.1.31</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9 10" type="primary">hisE</name>
        <name evidence="10" type="ORF">J422_04033</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>1-(5-phospho-beta-D-ribosyl)-ATP + H2O = 1-(5-phospho-beta-D-ribosyl)-5'-AMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:22828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="ChEBI" id="CHEBI:73183"/>
            <dbReference type="EC" id="3.6.1.31"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 9">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/9.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the PRA-PH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.31" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000025">
        <property type="protein sequence ID" value="ENN96136.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591461.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000025.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSJ4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00736"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.790"/>
    <dbReference type="OrthoDB" id="39686at2157"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00007"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd11534">
        <property type="entry name" value="NTP-PPase_HisIE_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.287.1080:FF:000002">
        <property type="entry name" value="Histidine biosynthesis bifunctional protein HisIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1080">
        <property type="entry name" value="MazG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01020">
        <property type="entry name" value="HisE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008179">
        <property type="entry name" value="HisE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021130">
        <property type="entry name" value="PRib-ATP_PPHydrolase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03188">
        <property type="entry name" value="histidine_hisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945:SF1">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01503">
        <property type="entry name" value="PRA-PH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101386">
        <property type="entry name" value="all-alpha NTP pyrophosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 9" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="6 9 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001460"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01020"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96136.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="96" mass="11130" checksum="8CF92331AD369A8D" modified="2013-06-26" version="1">MNMLYEVFNIIKERIKEKPEGSYVAKLTTDDEKKAINKICEKIGEEAAELILAAKDNIKEEIIKESADLIFHVMVLLAYSGVDLDELIKEFERRRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSN0</accession>
    <name>N6VSN0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Ribosomal protein L19e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="primary">rpl19e</name>
        <name evidence="3" type="ORF">J422_03998</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic ribosomal protein eL19 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96186.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591417.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSN0"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1650.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035970">
        <property type="entry name" value="60S_ribosomal_eL19_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023638">
        <property type="entry name" value="Ribosomal_eL19_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015972">
        <property type="entry name" value="Ribosomal_eL19_dom1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057259">
        <property type="entry name" value="Ribosomal_L19e"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01280">
        <property type="entry name" value="Ribosomal_L19e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48140">
        <property type="entry name" value="Ribosomal protein L19 (L19e)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00526">
        <property type="entry name" value="RIBOSOMAL_L19E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Ribosomal protein L19e N-terminal" evidence="2">
        <location>
            <begin position="2"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="37"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU000574"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01280"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96186.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="37" mass="4352" checksum="680326B9810260D2" modified="2013-06-26" version="1" fragment="single">MNLTVQRRLAAKILKCGLDRVWIDPEHIEDVKMAMTR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSN2</accession>
    <name>N6VSN2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Carboxymuconolactone decarboxylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03543</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96191.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591234.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSN2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01394"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.691"/>
    <dbReference type="OrthoDB" id="73788at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051920">
        <property type="term" value="F:peroxiredoxin activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1290.10">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029032">
        <property type="entry name" value="AhpD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003779">
        <property type="entry name" value="CMD-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33930">
        <property type="entry name" value="ALKYL HYDROPEROXIDE REDUCTASE AHPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33930:SF2">
        <property type="entry name" value="BLR3452 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02627">
        <property type="entry name" value="CMD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69118">
        <property type="entry name" value="AhpD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Carboxymuconolactone decarboxylase-like" evidence="1">
        <location>
            <begin position="19"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02627"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96191.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="108" mass="12180" checksum="85F68F2CBEA3119A" modified="2013-06-26" version="1">MKGVFYADSLKFLLEEDKELFEVIKELNEKVYTGKVLDYKTLKIIAIAIAAAKGDEKALKKQIVSGMKELNITKDEVIDALRVVLLTTGMPNFMKGMRVLKEVLEGKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSP8</accession>
    <name>N6VSP8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Alpha-glucan phosphorylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03663</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycogen phosphorylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96211.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591272.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSP8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01372"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.715"/>
    <dbReference type="OrthoDB" id="17863at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008184">
        <property type="term" value="F:glycogen phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011834">
        <property type="entry name" value="Agluc_phsphrylas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000811">
        <property type="entry name" value="Glyco_trans_35"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052182">
        <property type="entry name" value="Glycogen/Maltodextrin_Phosph"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02094">
        <property type="entry name" value="more_P_ylases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42655">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42655:SF1">
        <property type="entry name" value="GLYCOGEN PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00343">
        <property type="entry name" value="Phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006047"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96211.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="519" mass="60492" checksum="A3142C117BA49072" modified="2013-06-26" version="1">MKPTAYFCMEFAIHQPLKTYAGGLGFLAGSHFRAAKRLNQPLVGVSILWSYGYYDQLRDKEGYMKVEYIRKYYDFLEDINLKIPITINNNTIWVKAYKLEEDVFNTCPVYFLTTDIPENDDFGRSITHKLYEANNIIHIAQEMVLGMAGYEVIKECENVEIYHLNEPHGLPLIFKLIEEHGLDYCKEHVVFTTHTPLPEGNETQDINLLKSMGFFGNVDVKLAERLGGNPFNYTVCALRACKRANAVSKRHKEVCDDMWKHVKDKCEIIAITNAQDKYYWQDPIIRDAAKKYDIDLLRERKMELKEILFEEVADQTGKIFKKDRLTVVWARRFTAYKRPYILLYDEPRLMELLKKKKIQVIWAGKPHPNDNNMIATFNWIVSKTRGMKRATILTGYELKLSKLLKQGSDIWLNTPKLRHEASGTSGMTASMNASIHMSTLDGWHVEWAAMYPDDSFTIGDGVRDDDAYVANCIYNLLEEVADIYDTEDWWFKCCNCVNHIVEYFDAERMAREYAEKMYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSV1</accession>
    <name>N6VSV1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 7 8">Chorismate synthase</fullName>
            <shortName evidence="7">CS</shortName>
            <ecNumber evidence="3 7 8">4.2.3.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5-enolpyruvylshikimate-3-phosphate phospholyase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">aroC</name>
        <name evidence="10" type="ORF">J422_03221</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the anti-1,4-elimination of the C-3 phosphate and the C-6 proR hydrogen from 5-enolpyruvylshikimate-3-phosphate (EPSP) to yield chorismate, which is the branch point compound that serves as the starting substrate for the three terminal pathways of aromatic amino acid biosynthesis. This reaction introduces a second double bond into the aromatic ring system.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7 9">
            <text>5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate</text>
            <dbReference type="Rhea" id="RHEA:21020"/>
            <dbReference type="ChEBI" id="CHEBI:29748"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57701"/>
            <dbReference type="EC" id="4.2.3.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7 9">
            <name>FMNH2</name>
            <dbReference type="ChEBI" id="CHEBI:57618"/>
        </cofactor>
        <text evidence="7 9">Reduced FMN (FMNH(2)).</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 7 9">Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7 9">Belongs to the chorismate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.3.5" evidence="3 7 8"/>
    <dbReference type="EMBL" id="APMM01000019">
        <property type="protein sequence ID" value="ENN96281.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590897.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSV1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00321"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.632"/>
    <dbReference type="OrthoDB" id="33049at2157"/>
    <dbReference type="UniPathway" id="UPA00053">
        <property type="reaction ID" value="UER00090"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004107">
        <property type="term" value="F:chorismate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009423">
        <property type="term" value="P:chorismate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07304">
        <property type="entry name" value="Chorismate_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.150.10">
        <property type="entry name" value="Chorismate synthase AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00300">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000453">
        <property type="entry name" value="Chorismate_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035904">
        <property type="entry name" value="Chorismate_synth_AroC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020541">
        <property type="entry name" value="Chorismate_synthase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00033">
        <property type="entry name" value="aroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003793">
        <property type="entry name" value="PRK05382.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21085:SF0">
        <property type="entry name" value="CHORISMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01264">
        <property type="entry name" value="Chorismate_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001456">
        <property type="entry name" value="Chorismate_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103263">
        <property type="entry name" value="Chorismate synthase, AroC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00787">
        <property type="entry name" value="CHORISMATE_SYNTHASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00788">
        <property type="entry name" value="CHORISMATE_SYNTHASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="5 7 9" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0274">FAD</keyword>
    <keyword evidence="7" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="7" id="KW-0288">FMN</keyword>
    <keyword evidence="6 7 9" id="KW-0456">Lyase</keyword>
    <keyword evidence="7" id="KW-0521">NADP</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="7">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>NADP(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58349"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="122"/>
            <end position="124"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="250"/>
            <end position="251"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="295"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="310"/>
            <end position="314"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005044"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00013036"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00300"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR00033"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU000605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96281.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="373" mass="41533" checksum="793A9EDAE682B761" modified="2013-06-26" version="1">MNTFGDMFRVTVFGESHGKAVGAVIDGCPANLPLTEEDIQKELDRRKPKSIFSTPRREEDKVEILSGIFENKTTGAPICGIVYNKNIRPKDYEEIKYKPRPGHADLVYRLKYKNYDYRGGGRASGRVTVGHVIGGAVAKKLLSYTYNIKIIGYTVKIGKIESEFNYYNNQEIFNNESAVEELINKIENNPLRCPSINEKEMEEYILKAIEEKDSVGGVVEIVILNLPVGVGNPIFNKLHAELGRALLSINAVKGVEVGRGFKSSEMFGSEMNDEMYFDDEKNIKFYSNNSGGVLGGISTGQPVVLRIAIKPTPSIGKRQRTINLKTLENDYIEIKGRHDPIIVPRVIPVAEAMVAITLADLMIKSGFIHPCCL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTE0</accession>
    <name>N6VTE0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 19">Tetrahydromethanopterin S-methyltransferase subunit B</fullName>
            <ecNumber evidence="18 19">7.2.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 19">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit B</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">mtrB</name>
        <name evidence="20" type="ORF">J422_02165</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 19">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 22">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="7.2.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 19">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 19">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Cell membrane</location>
            <topology evidence="2 19">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the MtrB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.1.4" evidence="18 19"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96456.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590238.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTE0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01181"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.425"/>
    <dbReference type="OrthoDB" id="114034at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01094">
        <property type="entry name" value="MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008690">
        <property type="entry name" value="MtrB_MeTrfase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04166">
        <property type="entry name" value="methano_MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002129">
        <property type="entry name" value="PRK00965.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05440">
        <property type="entry name" value="MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005518">
        <property type="entry name" value="MtrB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 19" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="9 19 20" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 19" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 19" id="KW-1278">Translocase</keyword>
    <keyword evidence="11 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="79"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00010027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ENN96456.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="107" mass="12456" checksum="1165F7B53CC6E8C9" modified="2013-06-26" version="1">MYVVKICPEIDVVMDVDSGLVAEMRKDMFMVDIIPVEERIKKLETLVNAFEKSLDPRNPPLNTFPNRDRVYEIAGHFKGLFFGFWIALVFMLIIIVALYKIFPGLFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTX1</accession>
    <name>N6VTX1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">TPR repeat-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_01418</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96616.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589945.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTX1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00005"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.278"/>
    <dbReference type="OrthoDB" id="115601at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019734">
        <property type="entry name" value="TPR_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050498">
        <property type="entry name" value="Ycf3"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858">
        <property type="entry name" value="TETRATRICOPEPTIDE REPEAT PROTEIN 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44858:SF1">
        <property type="entry name" value="UDP-N-ACETYLGLUCOSAMINE--PEPTIDE N-ACETYLGLUCOSAMINYLTRANSFERASE SPINDLY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12895">
        <property type="entry name" value="ANAPC3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00515">
        <property type="entry name" value="TPR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13181">
        <property type="entry name" value="TPR_8"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00028">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50005">
        <property type="entry name" value="TPR"/>
        <property type="match status" value="3"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <keyword evidence="2 3" id="KW-0802">TPR repeat</keyword>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="118"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="152"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="repeat" description="TPR" evidence="3">
        <location>
            <begin position="186"/>
            <end position="219"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022803"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96616.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="286" mass="33233" checksum="9AD7B38558AC7FB4" modified="2013-06-26" version="1">MKNDIFKKFIKIIDLIEGGEFKTAEEELLKLSEEYPNNPLIYYLLGKVNTYKKFSKLLKFEKVHIPIEFLKYPKTTYDILKLALTPEYREAMLSIERGDYYKAIEDFKKLTKIDGEFVTPWLYKALLYEMIGDLENALMAINKVLELNKKDALAWYIKGRILRKLGRYNEALEALYNAITLDKNLVNVLKELGYTNLIIGDYKKALKWLNDYLKKVPNDPEALFYKAIAYKGLGKLEEALKILDGIIDKGTNVFIKTTSMLIKASILERLGKVEEAVKVYNEILKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU05</accession>
    <name>N6VU05_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Large ribosomal subunit protein eL40</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3 5" type="primary">rpl40e</name>
        <name evidence="5" type="ORF">J422_01176</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the eukaryotic ribosomal protein eL40 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96661.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589901.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU05"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00809"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.230"/>
    <dbReference type="OrthoDB" id="45138at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.1060.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00788">
        <property type="entry name" value="Ribosomal_eL40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023657">
        <property type="entry name" value="Ribosomal_eL40_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001975">
        <property type="entry name" value="Ribosomal_eL40_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038587">
        <property type="entry name" value="Ribosomal_eL40_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003161">
        <property type="entry name" value="PRK04136.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39649">
        <property type="entry name" value="50S RIBOSOMAL PROTEIN L40E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39649:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL40"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01020">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01377">
        <property type="entry name" value="Ribosomal_L40e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="1 3 5" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein eL40" evidence="4">
        <location>
            <begin position="3"/>
            <end position="45"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM01377"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96661.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="47" mass="5655" checksum="783CFCA5FCCB9693" modified="2013-06-26" version="1">MPFEEAIKRIFNKKICMRCNARNPWRAEKCRKCGYTKLRPKAKEPRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VWX8</accession>
    <name>N6VWX8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">UPF0200 protein J422_06937</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_06937</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="3">Belongs to the UPF0200 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000065">
        <property type="protein sequence ID" value="ENN95602.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594630.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VWX8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00854"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1344"/>
    <dbReference type="OrthoDB" id="85381at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01111">
        <property type="entry name" value="UPF0200"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022970">
        <property type="entry name" value="NTP_hydrolase-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930:SF1">
        <property type="entry name" value="DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41930">
        <property type="entry name" value="UPF0200 PROTEIN MJ1399"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13207">
        <property type="entry name" value="AAA_17"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95602.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="195" mass="22645" checksum="A654C5050A6BF5A8" modified="2013-06-26" version="1">MLLIGVTGMPGAGKNAVYNIAEELDIPIVSMGDVVRYETKKRSLPLTPENVGNIAIKLREEFGNEAIAVVTLKYIEEHFKDKEIIIIEGIRSLYEVNYFRKHYPLVLIAIHSSPITRFERLKRRGREDDPNTWDVFVERDLRELKFGIGEAIAIADFMVVNEDGYEEYLNNLKEVILKIVKNKDKFMEYNFIYKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXB9</accession>
    <name>N6VXB9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Proline--tRNA ligase</fullName>
            <ecNumber evidence="9">6.1.1.15</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">Prolyl-tRNA synthetase</fullName>
            <shortName evidence="9">ProRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">proS</name>
        <name evidence="11" type="ORF">J422_06015</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 9">
            <text>tRNA(Pro) + L-proline + ATP = L-prolyl-tRNA(Pro) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:14305"/>
            <dbReference type="Rhea" id="RHEA-COMP:9700"/>
            <dbReference type="Rhea" id="RHEA-COMP:9702"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:60039"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78532"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.15"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">Consists of three domains: the N-terminal catalytic domain, the anticodon-binding domain and the C-terminal extension.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.15" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000048">
        <property type="protein sequence ID" value="ENN95767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593139.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXB9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00923"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1171"/>
    <dbReference type="OrthoDB" id="7375at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017101">
        <property type="term" value="C:aminoacyl-tRNA synthetase multienzyme complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004827">
        <property type="term" value="F:proline-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006433">
        <property type="term" value="P:prolyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00778">
        <property type="entry name" value="ProRS_core_arch_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.930.10:FF:000037">
        <property type="entry name" value="Proline--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.800">
        <property type="entry name" value="Anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.30">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01571">
        <property type="entry name" value="Pro_tRNA_synth_type3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002314">
        <property type="entry name" value="aa-tRNA-synt_IIb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006195">
        <property type="entry name" value="aa-tRNA-synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004154">
        <property type="entry name" value="Anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036621">
        <property type="entry name" value="Anticodon-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002316">
        <property type="entry name" value="Pro-tRNA-ligase_IIa"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004499">
        <property type="entry name" value="Pro-tRNA-ligase_IIa_arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017449">
        <property type="entry name" value="Pro-tRNA_synth_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015264">
        <property type="entry name" value="Pro-tRNA_synth_II_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033721">
        <property type="entry name" value="ProRS_core_arch_euk"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00408">
        <property type="entry name" value="proS_fam_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382:SF2">
        <property type="entry name" value="BIFUNCTIONAL GLUTAMATE_PROLINE--TRNA LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43382">
        <property type="entry name" value="PROLYL-TRNA SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03129">
        <property type="entry name" value="HGTP_anticodon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09181">
        <property type="entry name" value="ProRS-C_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00587">
        <property type="entry name" value="tRNA-synt_2b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01046">
        <property type="entry name" value="TRNASYNTHPRO"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64586">
        <property type="entry name" value="C-terminal domain of ProRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52954">
        <property type="entry name" value="Class II aaRS ABD-related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50862">
        <property type="entry name" value="AA_TRNA_LIGASE_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 9" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 9 11" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 9" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-transfer RNA synthetases class-II family profile" evidence="10">
        <location>
            <begin position="31"/>
            <end position="273"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50862"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="451" mass="53133" checksum="E72625C2B308DF59" modified="2013-06-26" version="1">MEFSEWYSDILERAEIFDVRYPIKGCGVYLPYGFKIRKYTFEIIRKLLDERGHDEVLFPLLIPEDLLAKEGEHIKGFEDEVFWVTHGGREKLEKKLALRPTSETAIYYMFKLWIKVHTDMPIKIYQVVNTFRYETKHTRPLIRLREIMTFKEAHTAHSTKEDAEKQVKEAIEIYKEFFDTLGIPYLISKRPSWDKFPGAEYTFAFDTIFPDGRAIQIATVHNLSQNFSKTFDIVFETPDGGKDYAYQTCYGISDRAIASIIAIHGDEKGLCLPPVVAPYQIVIVPLIFKDNKEPLNKAKELKEKLKSYRVYLDDRDIRPGRKFNEWELRGVPLRIEIGPKDIENKKITIFRRDTMEKFQICEDELEKIDEILNDIMENMRKKAIKKFEDFITIMDYDVDRIKETLNNKRGIILIPFDESIYNSELEEKVEARVLGETEYKGKKYIAIAKTY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXC0</accession>
    <name>N6VXC0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Queuosine synthesis-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06040</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000048">
        <property type="protein sequence ID" value="ENN95772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593150.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXC0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00928"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1176"/>
    <dbReference type="OrthoDB" id="85793at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012096">
        <property type="entry name" value="ATPase_PP-loop_MJ1638"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052188">
        <property type="entry name" value="Ni-pincer_cofactor_biosynth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169:SF1">
        <property type="entry name" value="ATPASE, PP-LOOP SUPERFAMILY-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43169">
        <property type="entry name" value="EXSB FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006601">
        <property type="entry name" value="ATPase_UCP006601"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="252" mass="29677" checksum="C886A5994C1D2FA3" modified="2013-06-26" version="1">MEERINRKLIEIRINNHIEILEKLDIDESLKKCLKEMLINRLVNKEFYKINFNEKENAILAYSGGVDSSVSLIIAKHLFNVKPITCYSPYIIKDKDVILKRAKKLGFNVEFINVDLEDVYKGTVEGRFHPCGRCHKIIEEVIVEKAIEEGIEYIIFGDLLAFGHLSFYKDRVYRLNLPSFFALTKNEEREILKKYGIEIKDKYGCPMLNEFHKIRGCKFTIQRILREVRARVIDYKEGARIIEEILNDRFPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXD6</accession>
    <name>N6VXD6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Molybdate/tungstate import ATP-binding protein WtpC</fullName>
            <ecNumber evidence="8">7.3.2.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_05993</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>tungstate(in) + ATP + H2O = tungstate(out) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:35027"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:46502"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.3.2.6"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">The complex is composed of two ATP-binding proteins (WtpC), two transmembrane proteins (WtpB) and a solute-binding protein (WtpA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the ABC transporter superfamily. Sulfate/tungstate importer (TC 3.A.1.6) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.3.2.6" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95787.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593055.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXD6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00560"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1167"/>
    <dbReference type="OrthoDB" id="18368at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901238">
        <property type="term" value="F:ABC-type tungstate transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050093">
        <property type="entry name" value="ABC_SmlMolc_Importer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781">
        <property type="entry name" value="SPERMIDINE/PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42781:SF4">
        <property type="entry name" value="SPERMIDINE_PUTRESCINE IMPORT ATP-BINDING PROTEIN POTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0500">Molybdenum</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="11">
        <location>
            <begin position="2"/>
            <end position="226"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022505"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00038307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00038781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00039025"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00041133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95787.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="289" mass="32730" checksum="662F92DB05625C78" modified="2013-06-26" version="1">MLIVKNLSKHWKEFKLIDVSFEIGNEYCIILGPSGAGKSVLLKCIAGILKPDKGKVILDGKDITSLSPEKRSIGYVPQNFALFPNMNVYKNIAYGLLLRGEDKEVVKRKVKELAEFLNISHLLDRDVKTLSGGEQQRVALARALILEPKILLLDEPTSSLDINIKEEVIKELKKINIPVLHVTHDLNEARTLAEKVGIFLNGKLIEFGKRDILYKPKKIETAEFLGFNIIDGKIISPYEIEITDGDYLVENVIDYHHYKKVFINYNGKIIKAFTKKDVKIGDRVGIKFE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXI3</accession>
    <name>N6VXI3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rpl4lp</name>
        <name evidence="7" type="synonym">rpl4</name>
        <name evidence="8" type="ORF">J422_05598</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Forms part of the polypeptide exit tunnel.</text>
    </comment>
    <comment type="function">
        <text evidence="7">One of the primary rRNA binding proteins, this protein initially binds near the 5'-end of the 23S rRNA. It is important during the early stages of 50S assembly. It makes multiple contacts with different domains of the 23S rRNA in the assembled 50S subunit and ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Part of the 50S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the universal ribosomal protein uL4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95842.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592682.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXI3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00274"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1092"/>
    <dbReference type="OrthoDB" id="10737at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1370.10:FF:000011">
        <property type="entry name" value="50S ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1370.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01328_A">
        <property type="entry name" value="Ribosomal_uL4_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002136">
        <property type="entry name" value="Ribosomal_uL4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023574">
        <property type="entry name" value="Ribosomal_uL4_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013000">
        <property type="entry name" value="Ribosomal_uL4_euk/arc_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045240">
        <property type="entry name" value="Ribosomal_uL4_euk/arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019970">
        <property type="entry name" value="Ribosomall_uL4-arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03672">
        <property type="entry name" value="rpl4p_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19431">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00573">
        <property type="entry name" value="Ribosomal_L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52166">
        <property type="entry name" value="Ribosomal protein L4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00939">
        <property type="entry name" value="RIBOSOMAL_L1E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010528"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01328"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95842.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="251" mass="28169" checksum="FEAABE1B5AC3E6C9" modified="2013-06-26" version="1">MEANVYNLNGEVIKKIELPEIFETEYRPDLIKRAFLSAFTAKLQPKGTDPLAGKRRSAESWGKGYGLARVPRLPGGRAALAPMAVGGRRAHPPKVEKILWERINKKERIKAIKSAIAATANIDLVRERGHRFSIEELPIVVENEFENIKKTKEVFEIFKKLKIDEDVIRAKEGKKIRAGKGKMRGRRYKKPKSVLVVVGDRCDAILACRNLAGVDVITAKDLSILHLAPGGKAGRLTLWTENAIEKLKERF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXK0</accession>
    <name>N6VXK0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL39</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5 6" type="primary">rpl39e</name>
        <name evidence="6" type="ORF">J422_05554</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL39 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000042">
        <property type="protein sequence ID" value="ENN95862.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592614.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXK0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00752"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1084"/>
    <dbReference type="OrthoDB" id="65887at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.1620.10:FF:000001">
        <property type="entry name" value="60S ribosomal protein-like L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1620.10">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00629">
        <property type="entry name" value="Ribosomal_eL39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000077">
        <property type="entry name" value="Ribosomal_eL39"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020083">
        <property type="entry name" value="Ribosomal_eL39_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023626">
        <property type="entry name" value="Ribosomal_eL39_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002316">
        <property type="entry name" value="PRK01242.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00832">
        <property type="entry name" value="Ribosomal_L39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48662">
        <property type="entry name" value="Ribosomal protein L39e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00051">
        <property type="entry name" value="RIBOSOMAL_L39E"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 6" id="KW-0689">Ribosomal protein</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009339"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00629"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95862.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="51" mass="6100" checksum="9CD0F234B5FB2BEA" modified="2013-06-26" version="1">MGSNKPLGKKLRLAKALKQNRRVPIFVILKTKGRVRFHPKLRHWRRKKLKA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VY15</accession>
    <name>N6VY15_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methanogenesis marker protein 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04755</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96022.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VY15"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00970"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.930"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2200">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017674">
        <property type="entry name" value="Methan_mark_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053870">
        <property type="entry name" value="TiaS-like_TCKD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03280">
        <property type="entry name" value="methan_mark_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705:SF2">
        <property type="entry name" value="DUF1743 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40705">
        <property type="entry name" value="TRNA(ILE2) 2-AGMATINYLCYTIDINE SYNTHETASE TIAS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22641">
        <property type="entry name" value="TiaS_TCKD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="TiaS-like TCKD" evidence="1">
        <location>
            <begin position="135"/>
            <end position="195"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF22641"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96022.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="295" mass="32752" checksum="C191B1E2F1F04D48" modified="2013-06-26" version="1">MYKRIIALVDEPLKRVELIEEHPCPNGNEWMIYQYKRTSPLIISAWREGNKHHFLVKIGKERLNLIPSLAAAGIEEVYVEGDRVHIIHAGLAGAGVGVELRKGAENVIEAKIIEKGGGSKLGKGEIVTRKMYKVIVGIDDTDTKDEGATWLLAHKIGLEIEKMGLGYYLDHTTVQLYPKNPEKTQNCVSIALSFAVFPEYRYKICKSIKKLLKENTLSDNTAMAIYYGLFPSKSLKLFALKAKKEIVSLEEAKLVALRNNVDVIEITGEKGIIGAVAALGLAEHHNIAPKLPEDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYA5</accession>
    <name>N6VYA5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Nitrogen regulatory protein P-II</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04463</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96092.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591817.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYA5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00631"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.875"/>
    <dbReference type="OrthoDB" id="146891at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011322">
        <property type="entry name" value="N-reg_PII-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019296">
        <property type="entry name" value="Unchr_N-regulatory-PII-rel"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10126">
        <property type="entry name" value="Nit_Regul_Hom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54913">
        <property type="entry name" value="GlnB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96092.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="110" mass="12480" checksum="CB8E0CCDB0D12329" modified="2013-06-26" version="1">MKILFYLFVESENIGKAINALSEGGITGFFLYDYKGMSPEEWKGFLIDEDPEKAIKAIQNLAKNAILIGTVIDEKKAIHIEKAIKERLKDCKYTIIQIPVLKIEVNYVGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYD3</accession>
    <name>N6VYD3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 5 6">RNA 3'-terminal phosphate cyclase</fullName>
            <shortName evidence="5">RNA cyclase</shortName>
            <shortName evidence="5">RNA-3'-phosphate cyclase</shortName>
            <ecNumber evidence="5 6">6.5.1.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rtcA</name>
        <name evidence="9" type="ORF">J422_04048</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the conversion of 3'-phosphate to a 2',3'-cyclic phosphodiester at the end of RNA. The mechanism of action of the enzyme occurs in 3 steps: (A) adenylation of the enzyme by ATP; (B) transfer of adenylate to an RNA-N3'P to produce RNA-N3'PP5'A; (C) and attack of the adjacent 2'-hydroxyl on the 3'-phosphorus in the diester linkage to produce the cyclic end product. The biological role of this enzyme is unknown but it is likely to function in some aspects of cellular RNA processing.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>a 3'-end 3'-phospho-ribonucleotide-RNA + ATP = a 3'-end 2',3'-cyclophospho-ribonucleotide-RNA + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:23976"/>
            <dbReference type="Rhea" id="RHEA-COMP:10463"/>
            <dbReference type="Rhea" id="RHEA-COMP:10464"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:83062"/>
            <dbReference type="ChEBI" id="CHEBI:83064"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.5.1.4"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the RNA 3'-terminal cyclase family. Type 1 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.5.1.4" evidence="5 6"/>
    <dbReference type="EMBL" id="APMM01000026">
        <property type="protein sequence ID" value="ENN96127.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYD3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00723"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.793"/>
    <dbReference type="OrthoDB" id="7994at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003963">
        <property type="term" value="F:RNA-3'-phosphate cyclase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006396">
        <property type="term" value="P:RNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00874">
        <property type="entry name" value="RNA_Cyclase_Class_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.65.10.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.360.20">
        <property type="entry name" value="RNA 3'-terminal phosphate cyclase, insert domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00200">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013791">
        <property type="entry name" value="RNA3'-term_phos_cycl_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023797">
        <property type="entry name" value="RNA3'_phos_cyclase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037136">
        <property type="entry name" value="RNA3'_phos_cyclase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000228">
        <property type="entry name" value="RNA3'_term_phos_cyc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017770">
        <property type="entry name" value="RNA3'_term_phos_cyc_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020719">
        <property type="entry name" value="RNA3'_term_phos_cycl-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013792">
        <property type="entry name" value="RNA3'P_cycl/enolpyr_Trfase_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036553">
        <property type="entry name" value="RPTC_insert"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03399">
        <property type="entry name" value="RNA_3prim_cycl"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096">
        <property type="entry name" value="RNA 3' TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11096:SF0">
        <property type="entry name" value="RNA 3'-TERMINAL PHOSPHATE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01137">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05189">
        <property type="entry name" value="RTC_insert"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005378">
        <property type="entry name" value="RNA3'_term_phos_cycl_euk"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55205">
        <property type="entry name" value="EPT/RTPC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01287">
        <property type="entry name" value="RTC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase" evidence="7">
        <location>
            <begin position="11"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="domain" description="RNA 3'-terminal phosphate cyclase insert" evidence="8">
        <location>
            <begin position="189"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="active site" description="Tele-AMP-histidine intermediate" evidence="5">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="279"/>
            <end position="283"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00021428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00200"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="NCBIfam" id="TIGR03399"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF01137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF05189"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96127.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="333" mass="37124" checksum="396774374E4D1546" modified="2013-06-26" version="1">MVIIIIIDGSYLEGGGQILRTSIALSSLLNKPVKIINIRKKRKNKGLAKQHLACIKAMKKLCNAEVEGLYLGSEEVTFIPSKLSPKDFTINIGSAGSVTLVIQTLLPLALGINKKFTVKIIGGTDVKYAPTVDYMKYITLKILENYGIKSELRLIRRGFYPEGGGEVIFTLFPSKIKMFDLVEHSGSREVFGIAYVQNLKEEIARRMRKRAVDLLNKYGFQPNIKVEVSKGISTGAGIFLSNDTIGSSYLGEKGLRAEIVAERATENLLKERKLALDRYMGDQIIPFLAFGKGKVGVSEITNHTKTNIWVVKHFLDVEFDIKEYKEGYIIEAL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYF5</accession>
    <name>N6VYF5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">CDP-archaeol synthase</fullName>
            <ecNumber evidence="11">2.7.7.67</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">carS</name>
        <name evidence="12" type="ORF">J422_03808</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the formation of CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol (CDP-archaeol) from 2,3-bis-(O-geranylgeranyl)-sn-glycerol 1-phosphate (DGGGP) and CTP. This reaction is the third ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + CTP + H(+) = CDP-2,3-bis-O-(geranylgeranyl)-sn-glycerol + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:25690"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="ChEBI" id="CHEBI:58838"/>
            <dbReference type="EC" id="2.7.7.67"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="11">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the CDP-archaeol synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.67" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96152.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591353.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYF5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00393"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.746"/>
    <dbReference type="OrthoDB" id="45383at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043338">
        <property type="term" value="F:CDP-2,3-bis-(O-geranylgeranyl)-sn-glycerol synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01117">
        <property type="entry name" value="CDP_archaeol_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032690">
        <property type="entry name" value="CarS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002726">
        <property type="entry name" value="CarS_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003114">
        <property type="entry name" value="PRK04032.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650">
        <property type="entry name" value="CDP-ARCHAEOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39650:SF1">
        <property type="entry name" value="CDP-ARCHAEOL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01864">
        <property type="entry name" value="CarS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="2 11" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="7 11" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="5 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="9 11" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 11" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 11" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="48"/>
            <end position="70"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="82"/>
            <end position="103"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="115"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="143"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96152.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="170" mass="19062" checksum="89A21FD319E52613" modified="2013-06-26" version="1">MKNIIISSIWYILPAYVANASACIFGGGSPLDNNAYFFDGKRILGDGVTYRGCFFGILFGTLTGIIQSIIHYIYFKNFTTDYIILAFLLSFGAIFGDAVGSFIKRRINIERGKPAPLLDQLDFVIFAIIFGSIIKPISFEEITVILILTVFIHILGNVIAYLLKIKDVWW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYK9</accession>
    <name>N6VYK9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">CoB/CoM heterodisulfide reductase subunit C</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_03668</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HdrC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96212.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591274.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYK9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01371"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.716"/>
    <dbReference type="OrthoDB" id="144910at2157"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017680">
        <property type="entry name" value="CoB/CoM_hetero-S_Rdtase_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051460">
        <property type="entry name" value="HdrC_iron-sulfur_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03290">
        <property type="entry name" value="CoB_CoM_SS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255:SF1">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43255">
        <property type="entry name" value="IRON-SULFUR-BINDING OXIDOREDUCTASE FADF-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0408">Iron</keyword>
    <keyword evidence="8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="26"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007097"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96212.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="185" mass="21111" checksum="53A7E3DBF4983FB6" modified="2013-06-26" version="1">MVIRTDKLNKNFVNIVAEEGKLVFGEHVGKSINACYQCGTCTGSCPSGRLTAYRTRKMIRKAQCGLEDVLDCDDMWYCTTCYTCHERCPREVKITDVIKILRNLASKKGNMALAHRKTASYVVKFGHAVPANKEIVELRRKLGLPPESPTAQFSKKDLEEVRTLIKELNFDKLIKFDWEKMDLCE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYZ3</accession>
    <name>N6VYZ3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL11</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpl11p</name>
        <name evidence="6" type="synonym">rpl11</name>
        <name evidence="10" type="ORF">J422_02664</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Forms part of the ribosomal stalk which helps the ribosome interact with GTP-bound translation factors.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the ribosomal stalk of the 50S ribosomal subunit. Interacts with L10 and the large rRNA to form the base of the stalk. L10 forms an elongated spine to which L12 dimers bind in a sequential fashion forming a multimeric L10(L12)X complex.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 7">Belongs to the universal ribosomal protein uL11 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96347.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590573.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYZ3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00085"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.522"/>
    <dbReference type="OrthoDB" id="8842at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015934">
        <property type="term" value="C:large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070180">
        <property type="term" value="F:large ribosomal subunit rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00349">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.10.250:FF:000006">
        <property type="entry name" value="50S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1550.10:FF:000007">
        <property type="entry name" value="50S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.250">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1550.10">
        <property type="entry name" value="Ribosomal protein L11/L12, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00736">
        <property type="entry name" value="Ribosomal_uL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000911">
        <property type="entry name" value="Ribosomal_uL11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020783">
        <property type="entry name" value="Ribosomal_uL11_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036769">
        <property type="entry name" value="Ribosomal_uL11_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020785">
        <property type="entry name" value="Ribosomal_uL11_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020784">
        <property type="entry name" value="Ribosomal_uL11_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036796">
        <property type="entry name" value="Ribosomal_uL11_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002232">
        <property type="entry name" value="PRK01143.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11661:SF1">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL11M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00298">
        <property type="entry name" value="Ribosomal_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03946">
        <property type="entry name" value="Ribosomal_L11_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00649">
        <property type="entry name" value="RL11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54747">
        <property type="entry name" value="Ribosomal L11/L12e N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46906">
        <property type="entry name" value="Ribosomal protein L11, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00359">
        <property type="entry name" value="RIBOSOMAL_L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6 7" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 7" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL11 N-terminal" evidence="9">
        <location>
            <begin position="6"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL11 C-terminal" evidence="8">
        <location>
            <begin position="70"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010537"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00736"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU003978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00298"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF03946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96347.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="164" mass="17947" checksum="BBDDB079EF718020" modified="2013-06-26" version="1">MGKEVVEVLVTGGRATAGPPLGPAIGPLGVNVMQVVKEINEKTKEYEGMQVPVKVIVDTETRKFEIEVGIPPTSALIKKELGIEKGAHEPRHEVVGNLTLEQAIKIAKMKRKSMLSYTLKDAVKEVLGTCGSMGVTVEGKDPKEVQKEIDQGVYDEIFKKYEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ57</accession>
    <name>N6VZ57_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11 12">4-hydroxy-tetrahydrodipicolinate synthase</fullName>
            <shortName evidence="11">HTPA synthase</shortName>
            <ecNumber evidence="3 11 12">4.3.3.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">dapA</name>
        <name evidence="16" type="ORF">J422_02514</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 11">Catalyzes the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to 4-hydroxy-tetrahydrodipicolinate (HTPA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 11">
            <text>L-aspartate 4-semialdehyde + pyruvate = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:34171"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:67139"/>
            <dbReference type="ChEBI" id="CHEBI:537519"/>
            <dbReference type="EC" id="4.3.3.7"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2 11">Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="11">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the DapA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Was originally thought to be a dihydrodipicolinate synthase (DHDPS), catalyzing the condensation of (S)-aspartate-beta-semialdehyde [(S)-ASA] and pyruvate to dihydrodipicolinate (DHDP). However, it was shown in E.coli that the product of the enzymatic reaction is not dihydrodipicolinate but in fact (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinic acid (HTPA), and that the consecutive dehydration reaction leading to DHDP is not spontaneous but catalyzed by DapB.</text>
    </comment>
    <dbReference type="EC" id="4.3.3.7" evidence="3 11 12"/>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96412.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590457.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ57"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00769"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.492"/>
    <dbReference type="OrthoDB" id="33636at2157"/>
    <dbReference type="UniPathway" id="UPA00034">
        <property type="reaction ID" value="UER00017"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008675">
        <property type="term" value="F:2-dehydro-3-deoxy-phosphogluconate aldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008840">
        <property type="term" value="F:4-hydroxy-tetrahydrodipicolinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019877">
        <property type="term" value="P:diaminopimelate biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009089">
        <property type="term" value="P:lysine biosynthetic process via diaminopimelate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00950">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00418">
        <property type="entry name" value="DapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005263">
        <property type="entry name" value="DapA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002220">
        <property type="entry name" value="DapA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020625">
        <property type="entry name" value="Schiff_base-form_aldolases_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020624">
        <property type="entry name" value="Schiff_base-form_aldolases_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00674">
        <property type="entry name" value="dapA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128:SF66">
        <property type="entry name" value="4-HYDROXY-2-OXOGLUTARATE ALDOLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12128">
        <property type="entry name" value="DIHYDRODIPICOLINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00701">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001365">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00146">
        <property type="entry name" value="DHPICSNTHASE"/>
    </dbReference>
    <dbReference type="SMART" id="SM01130">
        <property type="entry name" value="DHDPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00665">
        <property type="entry name" value="DHDPS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00666">
        <property type="entry name" value="DHDPS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 11" id="KW-0220">Diaminopimelate biosynthesis</keyword>
    <keyword evidence="8 11 16" id="KW-0456">Lyase</keyword>
    <keyword evidence="7 11" id="KW-0457">Lysine biosynthesis</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 11" id="KW-0704">Schiff base</keyword>
    <feature type="active site" description="Proton donor/acceptor" evidence="11 13">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="11 13">
        <location>
            <position position="160"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="43"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11 14">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>pyruvate</name>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
        </ligand>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="11 15">
        <location>
            <position position="42"/>
        </location>
    </feature>
    <feature type="site" description="L-lysine inhibitor binding; via carbonyl oxygen" evidence="15">
        <location>
            <position position="47"/>
        </location>
    </feature>
    <feature type="site" description="L-lysine inhibitor binding" evidence="15">
        <location>
            <position position="78"/>
        </location>
    </feature>
    <feature type="site" description="L-lysine inhibitor binding" evidence="15">
        <location>
            <position position="82"/>
        </location>
    </feature>
    <feature type="site" description="L-lysine inhibitor binding" evidence="15">
        <location>
            <position position="104"/>
        </location>
    </feature>
    <feature type="site" description="Part of a proton relay during catalysis" evidence="11 15">
        <location>
            <position position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012086"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00418"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="NCBIfam" id="TIGR00674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001365-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN96412.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="287" mass="31366" checksum="50EB9301B22080FD" modified="2013-06-26" version="1">MYGVFPAIITPFKNGNIDYESLERHINFLIDNGVDGIVVAGTTGESATLSHDEHKELIERSVEIVNGRVKLVAGAGSNSTKEAVELSTFAEDVGADAVLSITPYYNKPTQQGLIKHFSEIAKAINLPIILYNVPSRTGINLEPETAKYLFDEFSNIVAIKEANPNLSQVSELVRYGITVLSGNDELTLPILALGGKGVISVVANITPKEMVELVHSALKGDFDKAKEIHYKLYPLMKALFIETNPIPVKTALSMLGRCSSELRSPLCEMSEKNKKILEKVLRDLGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZB7</accession>
    <name>N6VZB7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Methyl-coenzyme M reductase operon protein C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_02195</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subunit">
        <text evidence="2 3">MCR is composed of three subunits: alpha, beta, and gamma. The function of proteins C and D is not known.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96462.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590265.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZB7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01187"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.431"/>
    <dbReference type="OrthoDB" id="113954at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007687">
        <property type="entry name" value="Me_CoM_Rdtase_prot-C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026327">
        <property type="entry name" value="Me_CoM_Rdtase_prot-C-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03264">
        <property type="entry name" value="met_CoM_red_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04609">
        <property type="entry name" value="MCR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF003137">
        <property type="entry name" value="McrC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00025920"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PIRNR" id="PIRNR003137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96462.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="198" mass="21454" checksum="65BA2D399432B87E" modified="2013-06-26" version="1">MPVGRKEQIVDCRAVMGLGEGGGLAQRGTLAEALRNDVIVVAMSPGRRHITKPVCEITYGIRETGIQTSVLVLNAGSGQAPDESGRSYGSTFGIKPEEAAQINRHKLCVVHFGNVISHIVHKARLLLKYVKIPTIIVCQAPLDMEDLAKAGVKTRDVKPVEPQTVGTVVDIVTGVVRGESCPQHKIDEIVMKIKKYLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZC3</accession>
    <name>N6VZC3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">RNA-processing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_02220</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96467.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590271.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZC3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01192"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.436"/>
    <dbReference type="OrthoDB" id="7870at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016779">
        <property type="term" value="F:nucleotidyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd22389">
        <property type="entry name" value="KH-I_Dim2p_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd22390">
        <property type="entry name" value="KH-I_Dim2p_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000076">
        <property type="entry name" value="KH domain protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1370.10:FF:000001">
        <property type="entry name" value="Polyribonucleotide nucleotidyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1370.10">
        <property type="entry name" value="K Homology domain, type 1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004088">
        <property type="entry name" value="KH_dom_type_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036612">
        <property type="entry name" value="KH_dom_type_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019964">
        <property type="entry name" value="KH_domain_protein_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055211">
        <property type="entry name" value="KH_PNO1_2nd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03665">
        <property type="entry name" value="arCOG04150"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010327">
        <property type="entry name" value="PRK13763.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826">
        <property type="entry name" value="RIBONUCLEASE Y"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12826:SF13">
        <property type="entry name" value="RNA-BINDING PROTEIN PNO1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00013">
        <property type="entry name" value="KH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22891">
        <property type="entry name" value="KH_PNO1_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54791">
        <property type="entry name" value="Eukaryotic type KH-domain (KH-domain type I)"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="8"/>
            <end position="77"/>
        </location>
    </feature>
    <feature type="domain" description="K Homology" evidence="5">
        <location>
            <begin position="91"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96467.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="208" mass="23651" checksum="1B64597965FA4E1D" modified="2013-06-26" version="1">MVFVNVEAKNVEILKVPKDRIGVLIGKKGEVKRAIEDTLGVKIKIDEDGNVFIYGTEKQKDPLALWKAKNIVKAISRGFNPEIALKLAKDDYVLEIINVDDYASSENAIRRLKGRVIGKEGKSRKYIEELTGTNLSIYGKTISIVGEVDSVQIAKEAVEMLLRGASHAKTYKFLERERQKIKAYKFELWEKRKVDKVYDELYKGEDDA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZU1</accession>
    <name>N6VZU1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Monovalent cation/H+ antiporter subunit E</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_01313</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96597.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589926.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZU1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00027"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.257"/>
    <dbReference type="OrthoDB" id="85180at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008324">
        <property type="term" value="F:monoatomic cation transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002758">
        <property type="entry name" value="Cation_antiport_E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004922">
        <property type="entry name" value="PRK06279.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584">
        <property type="entry name" value="NA(+)/H(+) ANTIPORTER SUBUNIT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34584:SF1">
        <property type="entry name" value="NA(+)_H(+) ANTIPORTER SUBUNIT E1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01899">
        <property type="entry name" value="MNHE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96597.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="99" mass="11256" checksum="B2BBD34C2868B524" modified="2013-06-26" version="1">MILATLGYLLVLLKAITESWIDVVKRCINGQIKPEVVEIESIINNPTGLILLSWSITATPGTLVIDLDTEKRKLKVACIYPRKRENIVPFEPYIKKMFD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZW1</accession>
    <name>N6VZW1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01233</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589912.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZW1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00042"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.241"/>
    <dbReference type="OrthoDB" id="65278at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="562" mass="64920" checksum="DDC0B648020CF23B" modified="2013-06-26" version="1">MKKLLIIALIISCVYAINYKYVSTVPIEVVNLSNINVNELEKYLNDNRLTLLILDERTKKDIIKALNITISPDKIPDYDNYTLITNSNCIVYPKKYVYYDDDGALIYIPPANISSKSINYTKISDGVYEIKKNKLLFLYPTDLNDKKTLDILGRYIAKNNGTFAYINKAPAGYDNILVAGVGVSKVIPDENGKYCIKVAKRKIKVNYLDDEVINKKIKAIKLLSAFGINVSYIVSGDEAIKLIEKSNLNKEDLKLINQYWFKSYKNYTHTYINPEKLNYDLDIVALSYYPLIYVDKSPETFENDPTVGGYYPTLINYESGYWQLGEISENRYYYLYKGEPYWNGKANEVSKWYYKGEPVPEKENNKMNDRYKYFNHWFVKNYGYALASGCNGLFLESSNEDLVNAVLGKDGDWKLDINGKVKYLIIPGKRGFEIYNGIPILKVPTPLSEVYGVNILNITYIPPEDEEFGVYIADIRHFNTNLLFKLKNEGTCIMNFNQMVNWLKDYYENNIHLINKSIIIVKNPKNVKITLYTNKQILGNFKHLIKNNGSYIIECPKVIILR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZW9</accession>
    <name>N6VZW9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Small ribosomal subunit protein uS10</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">rps10p</name>
        <name evidence="5" type="synonym">rps10</name>
        <name evidence="7" type="ORF">J422_01293</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Involved in the binding of tRNA to the ribosomes.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the universal ribosomal protein uS10 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96632.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589922.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZW9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00031"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.253"/>
    <dbReference type="OrthoDB" id="371736at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015935">
        <property type="term" value="C:small ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.600:FF:000004">
        <property type="entry name" value="30S ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.600">
        <property type="entry name" value="Ribosomal protein S10 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00508">
        <property type="entry name" value="Ribosomal_uS10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001848">
        <property type="entry name" value="Ribosomal_uS10"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018268">
        <property type="entry name" value="Ribosomal_uS10_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027486">
        <property type="entry name" value="Ribosomal_uS10_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036838">
        <property type="entry name" value="Ribosomal_uS10_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005729">
        <property type="entry name" value="Ribosomal_uS10_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01049">
        <property type="entry name" value="rpsJ_bact"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01046">
        <property type="entry name" value="uS10_euk_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11700">
        <property type="entry name" value="30S RIBOSOMAL PROTEIN S10 FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00338">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00971">
        <property type="entry name" value="RIBOSOMALS10"/>
    </dbReference>
    <dbReference type="SMART" id="SM01403">
        <property type="entry name" value="Ribosomal_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54999">
        <property type="entry name" value="Ribosomal protein S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00361">
        <property type="entry name" value="RIBOSOMAL_S10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Small ribosomal subunit protein uS10" evidence="6">
        <location>
            <begin position="5"/>
            <end position="99"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00508"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM01403"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96632.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="103" mass="11801" checksum="5E191D8C3EA14B48" modified="2013-06-26" version="1">MQVARIKLTSTDHKILDDVCRQIREIAEKTGVDISGPIPLPTKKLKVTVRRSPDGEGSSTFDRFTMKIHKRLIEIDADERALRHIVKIKIPDNVQIEIQFVNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6W024</accession>
    <name>N6W024_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Methanogenesis marker protein 2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00611</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589802.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W024"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00911"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.117"/>
    <dbReference type="OrthoDB" id="31494at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009030">
        <property type="term" value="F:thiamine-phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009228">
        <property type="term" value="P:thiamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02192">
        <property type="entry name" value="PurM-like3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1330.10:FF:000027">
        <property type="entry name" value="Thiamine monophosphate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017668">
        <property type="entry name" value="Methan_mark_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006283">
        <property type="entry name" value="ThiL-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011413">
        <property type="entry name" value="UCP036540_AIR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03267">
        <property type="entry name" value="methan_mark_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30270:SF0">
        <property type="entry name" value="THIAMINE-MONOPHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036540">
        <property type="entry name" value="UCP036540_AIR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="1">
        <location>
            <begin position="44"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="2">
        <location>
            <begin position="191"/>
            <end position="305"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="323" mass="35974" checksum="392EC0FEE0DF8CEA" modified="2013-06-26" version="1">MLKKIIEEIKNFEGVIRKREIKEVVNIINFIDSDYEFDIIVDYGDDAAVIGIDGKNAILLAADGIWGKLLEADPWWAGYCSVLVNCKDIAAMGGKSIGMTNILSIKDKETCKMVLKGVKDGVKKFGVPMVGGHTHPDAQCNVLDVAITGIAKRDKILRSDNAKVGDRIIFAYDLDGRLYDKFPLNWDTTTMKSKKIVRAQMDVLIDIAENNLANSCKDISNPGAIGTLGMLLEVSKKGGVVDLLKIPKPDNIDLKHWLKVYPGSGYVLTARKENVNEIKKLFEDVGMTAEDCGEIIKDRKLYLKYKDEKDIVFDFEKEYISGC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6W066</accession>
    <name>N6W066_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">MrpA C-terminal/MbhD domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_00506</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96752.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589783.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W066"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00053"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.97"/>
    <dbReference type="OrthoDB" id="65655at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1200">
        <property type="entry name" value="NADH-ubiquinone/plastoquinone oxidoreductase chain 6, subunit NuoJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025383">
        <property type="entry name" value="MrpA_C/MbhD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042106">
        <property type="entry name" value="Nuo/plastoQ_OxRdtase_6_NuoJ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004923">
        <property type="entry name" value="PRK06280.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13244">
        <property type="entry name" value="MbhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="6"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="29"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="52"/>
            <end position="71"/>
        </location>
    </feature>
    <feature type="domain" description="MrpA C-terminal/MbhD" evidence="7">
        <location>
            <begin position="10"/>
            <end position="76"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96752.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="79" mass="8716" checksum="9AE0735FF27DBBF8" modified="2013-06-26" version="1">MEIIHYIVILLMLLSSLAALLQKDLIKSIILSGFSGLCLAYLYYALLAPDVALTEAILGGAILPALFAFTIRRTERFED</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6W077</accession>
    <name>N6W077_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Respiratory-chain NADH dehydrogenase subunit 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_00581</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96767.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589798.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W077"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00068"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.112"/>
    <dbReference type="OrthoDB" id="15253at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052561">
        <property type="entry name" value="ComplexI_Subunit1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001694">
        <property type="entry name" value="NADH_UbQ_OxRdtase_su1/FPO"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43359">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43359:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 4-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00146">
        <property type="entry name" value="NADHdh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="77"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="164"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="205"/>
            <end position="234"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="241"/>
            <end position="261"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="267"/>
            <end position="286"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96767.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="288" mass="31549" checksum="A110F1FF78BED0D2" modified="2013-06-26" version="1">MDMSIIYILNLTFHAFLVGSLLLGFHRKLIARIQGRPGPPVIQYLLHTIKFYFKEITFPITAGNPLYIFIPLFDLSIWLIALIIAVVLKSSLLILIGIYILQKIVEHGCGLSSGSPYGKMGGVRSVFSAAAEVPLFSAVAVVYLAVKSTLISDIIAYQQTNGALIFKLPIAAFAFFILLASKSPYSPFGIVKGKDIVSGYMTEHYGLLASIIYIAESISYYVLLWLFITVFLGITGIIPTLAAMVVLTIILSFISALTPLLAPHHSVMLQMSIAGLVLIDVIYRLLAY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTF3</accession>
    <name>N6UTF3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">LarC family nickel insertion protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_07067</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000071">
        <property type="protein sequence ID" value="ENN95579.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTF3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01228"/>
    <dbReference type="OrthoDB" id="10691at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002822">
        <property type="entry name" value="Ni_insertion"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00299">
        <property type="entry name" value="nickel pincer cofactor biosynthesis protein LarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36566">
        <property type="entry name" value="NICKEL INSERTION PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36566:SF1">
        <property type="entry name" value="PYRIDINIUM-3,5-BISTHIOCARBOXYLIC ACID MONONUCLEOTIDE NICKEL INSERTION PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01969">
        <property type="entry name" value="Ni_insertion"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0533">Nickel</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95579.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="227" mass="25400" checksum="6B4ACD18B698DFFC" modified="2013-06-26" version="1" fragment="single">MFLIDPFSGISGDMFIAAFSEFIDKNELIKIINKVINAKIDIKKVKKNGIIANKLIISKKDKNLSYNEMKEIILSANIDNRIKNHAISIINILAKAEAKIHGIDLDEVHFHEISEIDTVIDALGAGYIIEKLNLKNNCYYLPINLGKGFVKITHGLYPIPAPATSEILKGFEVFYYGEGELTTPTGAAILRYINPTLLKGSFTYTKVSYGAGDREYELLNTLRVFKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTH3</accession>
    <name>N6UTH3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Serine dehydrogenase proteinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06907</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000064">
        <property type="protein sequence ID" value="ENN95609.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTH3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00930"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002825">
        <property type="entry name" value="Pept_S49_ser-pept_pro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35984">
        <property type="entry name" value="PERIPLASMIC SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35984:SF4">
        <property type="entry name" value="PERIPLASMIC SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01972">
        <property type="entry name" value="SDH_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="114"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95609.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="114" mass="13128" checksum="44FCD9119874F354" modified="2013-06-26" version="1" fragment="single">MFDIISLFWWLFIFYLIMAPQIQYKQLQLIRLKLLNKISEKRNSTVITLIHRQESVGLFGIPIYKFISIEDSEDILRAIRSAPKDKPIDLIIHTPGGLVLAATQIAKALKEHPA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTY1</accession>
    <name>N6UTY1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-oxoglutarate synthase subunit KorA</fullName>
            <ecNumber evidence="4">1.2.7.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">2-ketoglutarate oxidoreductase alpha chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">2-oxoglutarate-ferredoxin oxidoreductase subunit alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_05703</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="2">
            <text>2 oxidized [2Fe-2S]-[ferredoxin] + 2-oxoglutarate + CoA = succinyl-CoA + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17297"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57292"/>
            <dbReference type="EC" id="1.2.7.3"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="3">Heterotetramer of the KorA, KorB, KorC and KorD subunits.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.3" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95809.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592795.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTY1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01262"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1112"/>
    <dbReference type="OrthoDB" id="31112at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047553">
        <property type="term" value="F:2-oxoglutarate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006082">
        <property type="term" value="P:organic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006979">
        <property type="term" value="P:response to oxidative stress"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044272">
        <property type="term" value="P:sulfur compound biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd07034">
        <property type="entry name" value="TPP_PYR_PFOR_IOR-alpha_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.970:FF:000022">
        <property type="entry name" value="2-oxoglutarate ferredoxin oxidoreductase alpha subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.920">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.970">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033412">
        <property type="entry name" value="PFOR_II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050722">
        <property type="entry name" value="Pyruvate:ferred/Flavod_OxRd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002880">
        <property type="entry name" value="Pyrv_Fd/Flavodoxin_OxRdtase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029061">
        <property type="entry name" value="THDP-binding"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009014">
        <property type="entry name" value="Transketo_C/PFOR_II"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006412">
        <property type="entry name" value="PRK08659.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154:SF14">
        <property type="entry name" value="2-OXOGLUTARATE SYNTHASE SUBUNIT KORA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32154">
        <property type="entry name" value="PYRUVATE-FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17147">
        <property type="entry name" value="PFOR_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01855">
        <property type="entry name" value="POR_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52518">
        <property type="entry name" value="Thiamin diphosphate-binding fold (THDP-binding)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52922">
        <property type="entry name" value="TK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate flavodoxin/ferredoxin oxidoreductase pyrimidine binding" evidence="8">
        <location>
            <begin position="13"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Pyruvate:ferredoxin oxidoreductase core" evidence="9">
        <location>
            <begin position="267"/>
            <end position="357"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00052359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00064882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00066947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00071398"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00076968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00079587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01855"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF17147"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95809.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="362" mass="40542" checksum="26E78235BE8795CC" modified="2013-06-26" version="1">MIAFLQGNEACAKGAIKAGCRFFAGYPITPSTEIAETMARELPKVNGYYIQMEDEIGSIAAVIGASWGGLKAMTATSGPGFSLMQENIGFACMTEAPCVIVNVQRGGPSTGQPTHASQGDMMQCRWGSHGDYEIIALAPSSVQEMYDFTIEAFNLSEKYRVPVFVMADEIVGHMREKVHLHDNFDIVNRELPKEKPCKKPYPFDVDVPPMPIFGEGYNVHVTGLTHDERGYPDVSPETHDKLVRRLVNKIRKNRNKIIKLEMHNIEAKTLFICYGSPSRTVKYVTNMLNKEGKDVGYIRLITVYPFPDDILKKVKAEKVIIPEMNLGQIYYEVERCCKNSDVILVDKIGGEIHKPDELMRVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTZ9</accession>
    <name>N6UTZ9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Flagellar assembly protein J</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_05818</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592832.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTZ9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01242"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1134"/>
    <dbReference type="OrthoDB" id="141855at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004704">
        <property type="entry name" value="PRK06041.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF2">
        <property type="entry name" value="FLAGELLA ACCESSORY PROTEIN J"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="9" id="KW-0966">Cell projection</keyword>
    <keyword evidence="9" id="KW-0969">Cilium</keyword>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="9" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="38"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="44"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="193"/>
            <end position="213"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="219"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="267"/>
            <end position="285"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="297"/>
            <end position="319"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="442"/>
            <end position="467"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="497"/>
            <end position="514"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="526"/>
            <end position="548"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="8">
        <location>
            <begin position="79"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="93"/>
            <end position="120"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="558" mass="63916" checksum="4B822BC7BB9E194C" modified="2013-06-26" version="1">MINLLLRIGMNPRDYLFKILLPAFIVSVILFIIGFLLFKGIVFYIFSLLPSIILLSALIYPYIILDTKKNKINERLHIFISKFGTLSITDLDRKELLKILSQEKEELDELAEESRKLYVLVSKWGRSLAEACRFLSQRTPSPEFADFLDRLAYAVDSGEELKEFLIKEQDIVMDDYAAFYKRTLYSLDLYKELYVSAMTSIAFFIAFSVLVPFLMPFDFVFMSTVGLFMLIATQMGVIVLIKGKVPFDRLWHTGKEPNETDLKLRRYLIISIILTILTLIFLIITKFILRMTPFYDIPYMILVAIGLTPLAIIGVKTYLEEERVKRKEYVYPDFLRSLGDSLSARGGGLKECLKYLSHHDFGPLTRDIKRLYKRVALSIDTTKSWRFFGIESCSYLIQLFSDMFSRCLYFGGDPKLAANIISKNFRKIIQLRKSKYQNVQQFVGVIYGLGAGLALALFASLGVAYMITNLYSSLNIPETVIQIIHVAPLTNAKLVEYILYGSLIIYAIMSSILIKILDGGHKYSAFLHFVIIVWICSLVAYGTKLLVLKVLGMSMPIY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUA4</accession>
    <name>N6UUA4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">DNA-directed RNA polymerase subunit beta</fullName>
            <ecNumber evidence="15">2.7.7.6</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">J422_05164</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">DNA-dependent RNA polymerase (RNAP) catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. The Rpo2 subunit (Rpo2N and Rpo2C in this organism) is implicated in DNA promoter recognition and in nucleotide binding.</text>
    </comment>
    <comment type="function">
        <text evidence="15">DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="22">
            <text>RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:21248"/>
            <dbReference type="Rhea" id="RHEA-COMP:14527"/>
            <dbReference type="Rhea" id="RHEA-COMP:17342"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61557"/>
            <dbReference type="ChEBI" id="CHEBI:140395"/>
            <dbReference type="EC" id="2.7.7.6"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="13">Part of the RNA polymerase complex.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="3 15">Belongs to the RNA polymerase beta chain family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.6" evidence="15"/>
    <dbReference type="EMBL" id="APMM01000033">
        <property type="protein sequence ID" value="ENN95929.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUA4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01224"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1010"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032549">
        <property type="term" value="F:ribonucleoside binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006351">
        <property type="term" value="P:DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00653">
        <property type="entry name" value="RNA_pol_B_RPB2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.270.10:FF:000011">
        <property type="entry name" value="DNA-directed RNA polymerase subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.150">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1070.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.270.10">
        <property type="entry name" value="DNA-directed RNA polymerase, subunit 2, domain 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1800.10">
        <property type="entry name" value="RNA polymerase alpha subunit dimerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015712">
        <property type="entry name" value="DNA-dir_RNA_pol_su2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007120">
        <property type="entry name" value="DNA-dir_RNAP_su2_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037033">
        <property type="entry name" value="DNA-dir_RNAP_su2_hyb_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007121">
        <property type="entry name" value="RNA_pol_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007646">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007647">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007641">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014724">
        <property type="entry name" value="RNA_pol_RPB2_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019969">
        <property type="entry name" value="RNAP_Rpo2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03670">
        <property type="entry name" value="rpoB_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20856">
        <property type="entry name" value="DNA-DIRECTED RNA POLYMERASE I SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04566">
        <property type="entry name" value="RNA_pol_Rpb2_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04567">
        <property type="entry name" value="RNA_pol_Rpb2_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00562">
        <property type="entry name" value="RNA_pol_Rpb2_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04560">
        <property type="entry name" value="RNA_pol_Rpb2_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF64484">
        <property type="entry name" value="beta and beta-prime subunits of DNA dependent RNA-polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01166">
        <property type="entry name" value="RNA_POL_BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="4 15 20" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 15 20" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="11 15" id="KW-0804">Transcription</keyword>
    <keyword evidence="6 15 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="18">
        <location>
            <begin position="7"/>
            <end position="68"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="19">
        <location>
            <begin position="89"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="DNA-directed RNA polymerase subunit 2 hybrid-binding" evidence="16">
        <location>
            <begin position="137"/>
            <end position="508"/>
        </location>
    </feature>
    <feature type="domain" description="RNA polymerase Rpb2" evidence="17">
        <location>
            <begin position="510"/>
            <end position="606"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00006835"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00025096"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00025838"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00048552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="RuleBase" id="RU363031"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF04560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="Pfam" id="PF04566"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="Pfam" id="PF04567"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ENN95929.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="619" mass="70438" checksum="9D75F3AD93D23679" modified="2013-06-26" version="1">MRREANIYVNGKLVGTTDRPEELVNYIREKRRRGEISPYTTVVYYEDSNDVHINTDAGRMVRPLIVVENGKPKLTKEHIEKLKKGEITFDDLVKEGVIEYLDAEEEENAYIALSEEELTEKHTHLEIDPLTILGIGAGIAPYPEHNSAPRITMAAAMGKQSLGIPMSNIKWRLDTRGHYLHYPQTPIVRTKHQEILGFEKRPAGQNFVVAIMSYEGFNMEDAIIFNKSAIDRGLARSTFFRTYDACERRYPGGQMDRFEIPDKGVRGYRSEEDYKKLDEDGIVSVETFVKGGEVIIGKTSPPRFLEEHEITIQTKPQRRDSSVVVRHGEQGYVDKVILTETKEGNRLVKVKVRDLRIPELGDKFASRHGQKGVIGLTVQQEDMPFTESGIVPDIIINPHGIPSRMTVGQLLEMLGGKVGALEGRIIDGTIFSGEEEMDLREALEKLGFKRSGKEVMYDGRTGKKYEVEIYIGVIYYQKLHHLVAGKIHARSRGPIQVLTRQPTEGRAREGGLRFGEMERDVLIGHGAAMLLKERLMDESDPYDVCVCSNCGDFAILDYRRNIKYCPICGEIELLYSNKKIPFIRIPYAFKLLLDELKSMCIQPKIKVRDKVELEDFKEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUB0</accession>
    <name>N6UUB0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Methyl-accepting chemotaxis sensory transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_05189</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000033">
        <property type="protein sequence ID" value="ENN95934.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592247.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000033.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUB0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01219"/>
    <dbReference type="OrthoDB" id="8523at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029095">
        <property type="entry name" value="NarX-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13675">
        <property type="entry name" value="PilJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158472">
        <property type="entry name" value="HAMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="12"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="195"/>
            <end position="248"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="233"/>
            <end position="260"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="293"/>
            <end position="344"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95934.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="351" mass="40290" checksum="D89BC8A32AF2739A" modified="2013-06-26" version="1" fragment="single">MFKNLSIKFKLLGIVSILVMCIIFGAAYTTILLKDMEHDANIINIAGKQRMLIQKMSKEAFMIALGNLEMKKELIKTAQEFDKNLNDLINGNEERGITPAPPIVKAQLLKVKSMWSEFYKNILIIYEKDPSDPEVKKALEYIKNHNMELLSEMHKAVLLYTEYSHEKVKELNLMLETMIILSIIISVLAFYVVKNHIIKPLEELNRVVLEIAKGNYNIKPKIKFRNDEIGVIFNKIKEIINNLKAEAEKEMKEKQMFDNAIRYMSEILDKVKEGNLNVKIKAKWEDERLNKLANLINSVIESLSEAIKELANEVRLLNEEIEKLKDIGERAKETSEQIADAANQVAVAATD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUG0</accession>
    <name>N6UUG0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_05035</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the autoinducer-2 exporter (AI-2E) (TC 2.A.86) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95979.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592131.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUG0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00482"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.987"/>
    <dbReference type="OrthoDB" id="137390at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002549">
        <property type="entry name" value="AI-2E-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716">
        <property type="entry name" value="TRANSMEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21716:SF71">
        <property type="entry name" value="TRANSPORT PROTEIN MJ1177-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01594">
        <property type="entry name" value="AI-2E_transport"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="9"/>
            <end position="26"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="32"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="61"/>
            <end position="87"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="135"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="192"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="221"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="253"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="291"/>
            <end position="324"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009773"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95979.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="9">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="331" mass="38476" checksum="7FF9999F2801B1BF" modified="2013-06-26" version="1">MRYNEFKYLIKIIIIVLILSFFYFIFPYIDVIAYAIVFSYMALPIYNFIYNISKNRNLASILAVIIFVLPIILIGLYIIWLIVSYIINLDIGLFISYYKWLVGKYRLDNIINDEFIKKYGEDVIKYIVSKISSSLLSLGNLFIKILIIIFMTFYFLKDGYHIKDIILNITPHDYRSRLEIYLMYLHETYKNIFISCVNISIIITALAYIGYKLTFSPYPELLAIITGIFALLPILGSWMVYGSLALYYFLINNYYSAIFILIYGVIFLNLVPDFILRPYLVKKDTDIHPVLVIIAFLIAPLSLGVVGFAIGPLVIGALYALYLAKYRDKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUJ3</accession>
    <name>N6UUJ3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 10">Isopentenyl phosphate kinase</fullName>
            <shortName evidence="10">IPK</shortName>
            <ecNumber evidence="2 10">2.7.4.26</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">J422_04690</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the formation of isopentenyl diphosphate (IPP), the building block of all isoprenoids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>isopentenyl phosphate + ATP = isopentenyl diphosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:33963"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:65078"/>
            <dbReference type="ChEBI" id="CHEBI:128769"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.26"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the isopentenyl phosphate kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.26" evidence="2 10"/>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96009.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591974.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUJ3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00956"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.917"/>
    <dbReference type="OrthoDB" id="15328at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102043">
        <property type="term" value="F:isopentenyl phosphate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016114">
        <property type="term" value="P:terpenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04241">
        <property type="entry name" value="AAK_FomA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024192">
        <property type="entry name" value="Fosfomycin_R_FomA-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040647">
        <property type="entry name" value="IPPK_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654">
        <property type="entry name" value="GLUTAMATE 5-KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43654:SF1">
        <property type="entry name" value="ISOPENTENYL PHOSPHATE KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016496">
        <property type="entry name" value="Kin_FomA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="8" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="6 10 14" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="13">
        <location>
            <begin position="2"/>
            <end position="210"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="4"/>
            <end position="8"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="46"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="50"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="143"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="163"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="200"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="204"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="12">
        <location>
            <position position="13"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012908"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00017267"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00049063"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PIRNR" id="PIRNR016496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRSR" id="PIRSR016496-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN96009.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="242" mass="27188" checksum="8BBC3A9268C9F8D2" modified="2013-06-26" version="1">MILKLGGSVLSDKKIPFSVNWDNLERIAMEIKKASVDDLIIVHGGGSFGHPIAKKYMKNGQFINMHKGFWEIQRAMRRFNNIIIDTLQSYDIPAVSIQPSSFVVYKGEIVFDVSAIKEFLKKKFVPVIHGDIIIGEDYKILSGDDIAPYLAKTLGLRLLYATDVDGVYVNGKVIERIDKENVEDVLKHLSGSNGIDVTGGMRYKIEMLIKNKLNAIIFNGNKRDNIYKALKGEKIGTEIILE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUU4</accession>
    <name>N6UUU4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Acetyl-CoA acetyltransferase</fullName>
            <ecNumber evidence="4">2.3.1.9</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_04133</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.9" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000027">
        <property type="protein sequence ID" value="ENN96119.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591628.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUU4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01348"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.809"/>
    <dbReference type="OrthoDB" id="167534at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003985">
        <property type="term" value="F:acetyl-CoA C-acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00829">
        <property type="entry name" value="SCP-x_thiolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.47.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002155">
        <property type="entry name" value="Thiolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016039">
        <property type="entry name" value="Thiolase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055140">
        <property type="entry name" value="Thiolase_C_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020616">
        <property type="entry name" value="Thiolase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004720">
        <property type="entry name" value="PRK06064.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870">
        <property type="entry name" value="ACETYL-COA C-ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42870:SF6">
        <property type="entry name" value="ACETYL-COA C-ACYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22691">
        <property type="entry name" value="Thiolase_C_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00108">
        <property type="entry name" value="Thiolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000429">
        <property type="entry name" value="Ac-CoA_Ac_transf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53901">
        <property type="entry name" value="Thiolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="1" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Thiolase N-terminal" evidence="2">
        <location>
            <begin position="4"/>
            <end position="221"/>
        </location>
    </feature>
    <feature type="domain" description="Thiolase C-terminal" evidence="3">
        <location>
            <begin position="240"/>
            <end position="385"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00108"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96119.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="388" mass="41878" checksum="46FE34EDAC5EA79F" modified="2013-06-26" version="1">MRDVAIIGYGQTAFGELWERSFRSLIVEAGVKAVEAAGIDGKDIDEIYIGNMSSGLFVGQEHVASLIADHAGLNPIAATRVEAACASGSLALRQAILNVASGASDIILVGGVEKMTDVVDPTKAISSAADQEWEAIFGATFPSLYAMMARRYMYEYGLTLEELSMWSVIMHEHGSKNKYAQFRNKVTLEQVMNSPIIADPLRLLHCSPISDGAAALVICEAEKAKEFVNSDDIIYIKASAQASDTIALHSREKITELKAAKIASEKAYKMAKIEAKDIDVSEVHDCFAINGLILLEDIGFCKKGEAKYLLDDIRIDGKKPVNPSGGLKAAGHALGATGIRQVGEIYWQLKRDKEVKDRQVEIKNGYGLAVNVGGTGGTVCVHILSDKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV50</accession>
    <name>N6UV50_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Restriction modification system DNA specificity domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_03433</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the type-I restriction system S methylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000021">
        <property type="protein sequence ID" value="ENN96239.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591066.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV50"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00938"/>
    <dbReference type="REBASE" id="68614">
        <property type="entry name" value="S.MviT80ORF3443P"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.671"/>
    <dbReference type="OrthoDB" id="84651at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009307">
        <property type="term" value="P:DNA restriction-modification system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.1120">
        <property type="entry name" value="Bipartite methylase S protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.220.20">
        <property type="entry name" value="DNA methylase specificity domains"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000055">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044946">
        <property type="entry name" value="Restrct_endonuc_typeI_TRD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052021">
        <property type="entry name" value="Type-I_RS_S_subunit"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30408:SF12">
        <property type="entry name" value="TYPE I RESTRICTION ENZYME MJAVIII SPECIFICITY SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30408">
        <property type="entry name" value="TYPE-1 RESTRICTION ENZYME ECOKI SPECIFICITY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01420">
        <property type="entry name" value="Methylase_S"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116734">
        <property type="entry name" value="DNA methylase specificity domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0680">Restriction system</keyword>
    <feature type="domain" description="Type I restriction modification DNA specificity" evidence="4">
        <location>
            <begin position="19"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Type I restriction modification DNA specificity" evidence="4">
        <location>
            <begin position="226"/>
            <end position="415"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010923"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96239.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="435" mass="50620" checksum="622B9A4A4FFCDF08" modified="2013-06-26" version="1">MVKFRWETEFKETEIGKIPKDWEVKRLGEIANISTGGTPSRKKKEYWNGKINWLKSKEVQDNYIYDTEEKITEKGMKNSNAKRLYPPGTLILAIYASPTAGRVAILKIPSTINQALAAIEGNNNKFLFFSLIGNRERLLLRASGAAQQNLNLEIVKNFEIPYPQPEEQTRISTVLSWFDDLIENKKKQNEILEKMAMAIFKSWFIDFEPFQDEEFVYNEELDMEIPKGWEVKPIGEVADATSGFSYKSSEKLESSEPESYVFITLNNTVEGGGFKPVYSWVKSERIKKKHLIKEGDLILPNTEQTKDARLLGSPGIVVFPPDYNKNYGVYSMDIVKVTPIKDHYKYYLYFNFRFNREEIATFHSGTNILHFKIQNFKKNYYLLIPPEPILQRFHSLVEPLFKKIILNQKQILTLKKIRDTLLPQLVFGRLRIEEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV81</accession>
    <name>N6UV81_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Succinate dehydrogenase/fumarate reductase iron-sulfur subunit</fullName>
            <ecNumber evidence="9">1.3.99.1</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_03383</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.99.1" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96269.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590985.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV81"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01062"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.664"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051537">
        <property type="term" value="F:2 iron, 2 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00207">
        <property type="entry name" value="fer2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1060.10">
        <property type="entry name" value="Alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036010">
        <property type="entry name" value="2Fe-2S_ferredoxin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001041">
        <property type="entry name" value="2Fe-2S_ferredoxin-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006058">
        <property type="entry name" value="2Fe2S_fd_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012675">
        <property type="entry name" value="Beta-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009051">
        <property type="entry name" value="Helical_ferredxn"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004489">
        <property type="entry name" value="Succ_DH/fum_Rdtase_Fe-S"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025192">
        <property type="entry name" value="Succ_DH/fum_Rdtase_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00384">
        <property type="entry name" value="dhsB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004898">
        <property type="entry name" value="PRK06259.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32479">
        <property type="entry name" value="GLYCOLATE OXIDASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32479:SF17">
        <property type="entry name" value="GLYCOLATE OXIDASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13085">
        <property type="entry name" value="Fer2_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13183">
        <property type="entry name" value="Fer4_8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54292">
        <property type="entry name" value="2Fe-2S ferredoxin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46548">
        <property type="entry name" value="alpha-helical ferredoxin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00197">
        <property type="entry name" value="2FE2S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51085">
        <property type="entry name" value="2FE2S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0001">2Fe-2S</keyword>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="2Fe-2S ferredoxin-type" evidence="7">
        <location>
            <begin position="1"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="169"/>
            <end position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022714"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96269.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="477" mass="55100" checksum="12D5C67A6CC3ED5E" modified="2013-06-26" version="1">MKIRIKRGNKIESYEVPEGLTVLEALEYINKKYNANILFRYGCRNGQCGSCALTIDNEPRLACITKVKDNMLIEPLKGFKVIKDLIVDREVYNKKLLSIKNYVVRRKYPEGLENINEDDVEKTKELRACIDCLSCLSQCPARDFNYSGATIMRQLLRFAYDVRDEEDRERIAFFENIYNCTTCAKCVEVCPQEINLVKAIENLRELCFKKGYYLDEHLKVRENILKGNRSVVKEGASLLDEIEEEYLVKDEDLRVAFFTGCLIDYRLKDVGKDTIKVLNHYNISVIIPKDQVCCGSPMFRTGQRDLAEKLKEKNIEVFEKLDVDYIITVCAGCGATLKKDYGLKNVRDITEIIYKKVKENRKNIKVTYHDPCHLLRGQGIYKEPREILKKTSNFIDLKACCCGAGGGLRSGRREIADAIGMRRAKMIYNTDAEIVVTVCPFCEYHLRECLEKYKNINKIDKEIKVMNIATFLSKYHL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVJ3</accession>
    <name>N6UVJ3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02794</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96369.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590618.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVJ3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00109"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.548"/>
    <dbReference type="OrthoDB" id="65823at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="156"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="23"/>
            <end position="82"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96369.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="178" mass="20640" checksum="5EAAE847D20B0D6C" modified="2013-06-26" version="1">MKDDWIENLLKDLNKSKSNDILIDEIINKLDSLISEINSYIAEIQYGLSITNEKINSSILELNKLENNLKNYMAVAEDIRESFENTYNTLIDIKGEIDNIRASIENSSNQMTRKYKNIENIISGYNKRIEMLDKYIKEIIKTQKNIINNLNTTRSLVYATILLTIINMLILLKITIFG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVK4</accession>
    <name>N6UVK4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Fluoride-specific ion channel FluC</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">fluC</name>
        <name evidence="8" type="synonym">crcB</name>
        <name evidence="9" type="ORF">J422_02854</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Fluoride-specific ion channel. Important for reducing fluoride concentration in the cell, thus reducing its toxicity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>fluoride(in) = fluoride(out)</text>
            <dbReference type="Rhea" id="RHEA:76159"/>
            <dbReference type="ChEBI" id="CHEBI:17051"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="7">
            <dbReference type="Rhea" id="RHEA:76160"/>
        </physiologicalReaction>
    </comment>
    <comment type="activity regulation">
        <text evidence="8">Na(+) is not transported, but it plays an essential structural role and its presence is essential for fluoride channel function.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cell membrane</location>
            <topology evidence="1 8">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6 8">Belongs to the fluoride channel Fluc/FEX (TC 1.A.43) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96379.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590638.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVK4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00120"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.560"/>
    <dbReference type="OrthoDB" id="253428at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0062054">
        <property type="term" value="F:fluoride channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140114">
        <property type="term" value="P:cellular detoxification of fluoride"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00454">
        <property type="entry name" value="FluC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003691">
        <property type="entry name" value="FluC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00494">
        <property type="entry name" value="crcB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR28259:SF1">
        <property type="entry name" value="FLUORIDE EXPORT PROTEIN 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02537">
        <property type="entry name" value="CRCB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 8" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="8" id="KW-0407">Ion channel</keyword>
    <keyword evidence="8" id="KW-0406">Ion transport</keyword>
    <keyword evidence="5 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8" id="KW-0915">Sodium</keyword>
    <keyword evidence="3 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="8" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="5"/>
            <end position="22"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="34"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="62"/>
            <end position="80"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <note>structural</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Na(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <note>structural</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00035120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00035585"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00454"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96379.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="123" mass="13723" checksum="2DCC87A07E86F842" modified="2013-06-26" version="1">MIREILLIGIGGFIGAVLRYLISGLIPYDFLPLGTLIVNMIGSFIIGFLMFSSLFLDIPMEYKLLIGTGFCGALTTFSTFSYETFYMIDEGLFLKAFINIILNVSLCLIMVFLGRTLAILLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVM1</accession>
    <name>N6UVM1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">SAM-dependent methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02939</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96394.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVM1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00136"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="152"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96394.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="152" mass="17203" checksum="8E72B0F11AE98C5A" modified="2013-06-26" version="1" fragment="single">MNYITPELAKKILNSKGEIYLNLDLNKTNKKFKVIVNEDKAIFPSGEIEIKILKKIAKDNAVYLLDNNRLYKLAIADESGYYKLVPTIPPTIEINGIRMHRTKGINPYEDTLNKVNSINIKKGDVVLDTCMGLGYTAIEAYRRGAKVITIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVN4</accession>
    <name>N6UVN4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Protease HtpX homolog</fullName>
            <ecNumber evidence="11">3.4.24.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">htpX</name>
        <name evidence="13" type="ORF">J422_02474</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="11">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="11">Cell membrane</location>
            <topology evidence="11">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 11">Belongs to the peptidase M48B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.24.-" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96404.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590440.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVN4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00778"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.484"/>
    <dbReference type="OrthoDB" id="28389at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004222">
        <property type="term" value="F:metalloendopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07336">
        <property type="entry name" value="M48B_HtpX_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2010.10">
        <property type="entry name" value="Metalloproteases ('zincins'), catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00188">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050083">
        <property type="entry name" value="HtpX_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022919">
        <property type="entry name" value="Pept_M48_protease_HtpX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001915">
        <property type="entry name" value="Peptidase_M48"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221">
        <property type="entry name" value="PROTEASE HTPX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43221:SF2">
        <property type="entry name" value="PROTEASE HTPX HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01435">
        <property type="entry name" value="Peptidase_M48"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10 11" id="KW-0472">Membrane</keyword>
    <keyword evidence="5 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 11" id="KW-0482">Metalloprotease</keyword>
    <keyword evidence="3 11" id="KW-0645">Protease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="13" id="KW-0346">Stress response</keyword>
    <keyword evidence="4 11" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="8 11" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="7 11" id="KW-0862">Zinc</keyword>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="35"/>
            <end position="57"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="141"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="11">
        <location>
            <begin position="177"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Peptidase M48" evidence="12">
        <location>
            <begin position="65"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="active site" evidence="11">
        <location>
            <position position="132"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="205"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>catalytic</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009779"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023049"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF01435"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96404.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="284" mass="32011" checksum="9C39112A5283BC50" modified="2013-06-26" version="1">MSFLIQAKTYLLMAFLIGLIYAICLLLHIPPIFAIIIALIPNLIAYFLSDKIVLASYGARILDEHEMPWLHDIVRRVARKAGIPKPKVAIVPIATPNAFATGRNPENAVVAVTEGILNILSPEELEGVIGHEIAHIKHRDILLATIVATLAGAITYIAEWFMWFGHFFVSEEEENPLQIVATLLLIILAPIAATLIQLAISRQREFYADEDGARWTHPLWLANALAKLERSVELYPLKEGNPATAHLFIVNPFRGDFIAKLFSTHPPTEERIRRLMEMYKRGWR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVV3</accession>
    <name>N6UVV3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 19">Tetrahydromethanopterin S-methyltransferase subunit E</fullName>
            <ecNumber evidence="18 19">7.2.1.4</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16 19">N5-methyltetrahydromethanopterin--coenzyme M methyltransferase subunit E</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="19" type="primary">mtrE</name>
        <name evidence="20" type="ORF">J422_02180</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 19">Part of a complex that catalyzes the formation of methyl-coenzyme M and tetrahydromethanopterin from coenzyme M and methyl-tetrahydromethanopterin. This is an energy-conserving, sodium-ion translocating step.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="17 19">
            <text>5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(in) = 5,6,7,8-tetrahydromethanopterin + methyl-coenzyme M + 2 Na(+)(out)</text>
            <dbReference type="Rhea" id="RHEA:53492"/>
            <dbReference type="ChEBI" id="CHEBI:29101"/>
            <dbReference type="ChEBI" id="CHEBI:58103"/>
            <dbReference type="ChEBI" id="CHEBI:58116"/>
            <dbReference type="ChEBI" id="CHEBI:58286"/>
            <dbReference type="ChEBI" id="CHEBI:58319"/>
            <dbReference type="EC" id="7.2.1.4"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 19">One-carbon metabolism; methanogenesis from CO(2); methyl-coenzyme M from 5,10-methylene-5,6,7,8-tetrahydromethanopterin: step 2/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5 19">The complex is composed of 8 subunits; MtrA, MtrB, MtrC, MtrD, MtrE, MtrF, MtrG and MtrH.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 19">Cell membrane</location>
            <topology evidence="2 19">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 19">Belongs to the MtrE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.1.4" evidence="18 19"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96459.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590253.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVV3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01184"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.428"/>
    <dbReference type="OrthoDB" id="82302at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00698"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0012506">
        <property type="term" value="C:vesicle membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030269">
        <property type="term" value="F:tetrahydromethanopterin S-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01098">
        <property type="entry name" value="MtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005780">
        <property type="entry name" value="MeTrfase_E"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01113">
        <property type="entry name" value="mtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04206">
        <property type="entry name" value="MtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016509">
        <property type="entry name" value="MtrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 19" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="15 19" id="KW-0472">Membrane</keyword>
    <keyword evidence="14 19" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="9 19 20" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="8 19" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10 19 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="12 19" id="KW-1278">Translocase</keyword>
    <keyword evidence="11 19" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="13 19" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="62"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="86"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="129"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="163"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="230"/>
            <end position="249"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="19">
        <location>
            <begin position="261"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00002533"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004839"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00009612"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011616"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00015120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00029819"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00044880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="ARBA" id="ARBA00044970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ENN96459.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="298" mass="31236" checksum="CEFFB7291CC23A77" modified="2013-06-26" version="1">MDPILALGAIALSGALATIAGCAEDIESDVGSQSNPNSQVQLAPQMGNIHRYYNKAISGEPVAYGLYVAVAGGVAYALINMGLPMLLALIAGTGVAALVHGAYAISAYLGRIVGQSKNFGQPVYWDIVMTHIGPIVGHGFIAVFCMLLMAYIANTILGNPFPLPLIALIFGITVGAIGSSTGDVHYGAEREYQKYPFGGGVPVANHGDIDIKAEIGLRNSMDSSYFCVKLGGPLTGLCFGLIVFLDGWRGVIGDVIKGNDIMASSIISIIIGLTIVAILAIINRKVEVFAREKYGPYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6UW49</accession>
    <name>N6UW49_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">DUF373 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02005</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96544.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590175.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UW49"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00246"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.393"/>
    <dbReference type="OrthoDB" id="31282at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007254">
        <property type="entry name" value="DUF373"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815:SF1">
        <property type="entry name" value="DUF373 FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38815">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED, DUF373 FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04123">
        <property type="entry name" value="DUF373"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="176"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="202"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="214"/>
            <end position="236"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="248"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="290"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="320"/>
            <end position="340"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96544.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="344" mass="39211" checksum="F41EB069AD3DA4AD" modified="2013-06-26" version="1">MKYLVLIVDMDDDLGRKAGIKTPILGREDNIKALIKLGIADPGDSDVNAILGGIKIYDELKAENKDVEIATISGYKDVESEKCALKVKEQLDFLIYMYNPNFIYLISDGKEDEVIVKYLEGKDIFIWKKRIVVKQSETLESTYYLIQEFIKKTMEEYIPLILTFVGFSLLLYAIFADIGWRLVIGLIGLYILSEGLGVKNIIFKKIKEGKELSFGRVFPISIIIVIFILTIGGVYTVSNLHATAINGYVGEFLLNYSNILLFSLIVLLVGKFIDTIIHTNEDILEILKKYFFYLICIFIVRELLLSGGEFLMRKLDFTGFAIRIIIYILVIMILSIILFVKVEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWP5</accession>
    <name>N6UWP5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00566</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589795.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWP5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00065"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.109"/>
    <dbReference type="OrthoDB" id="65410at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019212">
        <property type="entry name" value="EhaG-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09878">
        <property type="entry name" value="EhaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="57"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="120"/>
            <end position="150"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="162"/>
            <end position="184"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="190"/>
            <end position="208"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="222" mass="24464" checksum="A7F9C24CB467E7EE" modified="2013-06-26" version="1">MINIFSLTILIALIIGFLSLLAIYFQKDDLHAFILTDIVESAMLIILAAISTDLAEALILPGLVVSLAELLAVSQVLLTRNSISKNNKKVDIFEEFRLPLQDKILKYDVEMEILETAPKFFAIILVIYGAILSGFTGGAVMASGLLFYAITQKAIGKELMNMWKGVTGLSGVAWALWIFGFLGFYIFPKYWLAFLLMAGLGLVIKVGYKLGLIGYVRGELDD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6V098</accession>
    <name>N6V098_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 6">Proteasome subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="4">20S proteasome alpha subunit</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="4">Proteasome core protein PsmA</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="4" type="primary">psmA</name>
        <name evidence="8" type="ORF">J422_06284</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4 6">Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation.</text>
    </comment>
    <comment type="activity regulation">
        <text evidence="4">The formation of the proteasomal ATPase PAN-20S proteasome complex, via the docking of the C-termini of PAN into the intersubunit pockets in the alpha-rings, triggers opening of the gate for substrate entry. Interconversion between the open-gate and close-gate conformations leads to a dynamic regulation of the 20S proteasome proteolysis activity.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4 6">The 20S proteasome core is composed of 14 alpha and 14 beta subunits that assemble into four stacked heptameric rings, resulting in a barrel-shaped structure. The two inner rings, each composed of seven catalytic beta subunits, are sandwiched by two outer rings, each composed of seven alpha subunits. The catalytic chamber with the active sites is on the inside of the barrel. Has a gated structure, the ends of the cylinder being occluded by the N-termini of the alpha-subunits. Is capped at one or both ends by the proteasome regulatory ATPase, PAN.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 4 6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 5 6">Belongs to the peptidase T1A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95738.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V098"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00281"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1224"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019773">
        <property type="term" value="C:proteasome core complex, alpha-subunit complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004298">
        <property type="term" value="F:threonine-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010498">
        <property type="term" value="P:proteasomal protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006511">
        <property type="term" value="P:ubiquitin-dependent protein catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03756">
        <property type="entry name" value="proteasome_alpha_archeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.60.20.10:FF:000004">
        <property type="entry name" value="Proteasome subunit alpha type-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.20.10">
        <property type="entry name" value="Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00289_A">
        <property type="entry name" value="Proteasome_A_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029055">
        <property type="entry name" value="Ntn_hydrolases_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050115">
        <property type="entry name" value="Proteasome_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023332">
        <property type="entry name" value="Proteasome_alpha-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019982">
        <property type="entry name" value="Proteasome_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000426">
        <property type="entry name" value="Proteasome_asu_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001353">
        <property type="entry name" value="Proteasome_sua/b"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03633">
        <property type="entry name" value="arc_protsome_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003075">
        <property type="entry name" value="PRK03996.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11599">
        <property type="entry name" value="PROTEASOME SUBUNIT ALPHA/BETA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00227">
        <property type="entry name" value="Proteasome"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10584">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00948">
        <property type="entry name" value="Proteasome_A_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56235">
        <property type="entry name" value="N-terminal nucleophile aminohydrolases (Ntn hydrolases)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00388">
        <property type="entry name" value="PROTEASOME_ALPHA_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51475">
        <property type="entry name" value="PROTEASOME_ALPHA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 4 5" id="KW-0647">Proteasome</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Proteasome alpha-type subunits" evidence="7">
        <location>
            <begin position="9"/>
            <end position="31"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022942"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU000552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS00388"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95738.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="251" mass="28105" checksum="34805EACEC5EAEED" modified="2013-06-26" version="1">MQIVPPTAYDRAITVFSPEGRLYQVEYAREAVRRGTTAIGITFKGGVILAVDRKITSKLVKIKSIEKIFRIDDHVIAATSGLVADARVLIDRARLEAQIYRLTYGEEIPIELLAKKICDIKQAYTQHGGVRPFGVSLLIAGIDKDNEPRLFETDPSGALIEYKATAIGSGRQIVMELLEKEYRDDIELDEALNLAIKGLTKANEEIKAENVDVCILTSTSGFERIEVDKIREIIESVKKELENENKEENKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0K3</accession>
    <name>N6V0K3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05628</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95848.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592692.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0K3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00268"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1098"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95848.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="88" mass="10518" checksum="A8582B98FC9CB27F" modified="2013-06-26" version="1">MNLIDLSIILILIVIVVMVIVVLKLKRIKKNLKIKARRMKWEKESTEIKLLKEIKKSMKCDEISNESFNNFIKKLEDNTYDDKLLKRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0R8</accession>
    <name>N6V0R8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05369</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95908.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592392.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0R8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01313"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1048"/>
    <dbReference type="OrthoDB" id="65551at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019208">
        <property type="entry name" value="DUF2097"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09870">
        <property type="entry name" value="DUF2097"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95908.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="82" mass="9816" checksum="D66448B3B4ABCE3A" modified="2013-06-26" version="1">MRIKLKPNEIFNYLNNIEEGDYIELYYGRVHIEGEFIEYYEGILKVLNKSFGIIELDVEEILEDILELAHTNKDGRKVIEFY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0Z7</accession>
    <name>N6V0Z7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 9 10">Prefoldin subunit beta</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 9">GimC subunit beta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pfdB</name>
        <name evidence="12" type="ORF">J422_05005</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 9">Molecular chaperone capable of stabilizing a range of proteins. Seems to fulfill an ATP-independent, HSP70-like function in archaeal de novo protein folding.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 9">Heterohexamer of two alpha and four beta subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 9">Belongs to the prefoldin subunit beta family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95973.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592118.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0Z7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00488"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.981"/>
    <dbReference type="OrthoDB" id="60701at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016272">
        <property type="term" value="C:prefoldin complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044183">
        <property type="term" value="F:protein folding chaperone"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051082">
        <property type="term" value="F:unfolded protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd23162">
        <property type="entry name" value="Prefoldin_beta_GimC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.370">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00307">
        <property type="entry name" value="PfdB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002777">
        <property type="entry name" value="PFD_beta-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012713">
        <property type="entry name" value="PfdB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009053">
        <property type="entry name" value="Prefoldin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02338">
        <property type="entry name" value="gimC_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903:SF0">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20903">
        <property type="entry name" value="PREFOLDIN SUBUNIT 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01920">
        <property type="entry name" value="Prefoldin_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46579">
        <property type="entry name" value="Prefoldin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 9" id="KW-0143">Chaperone</keyword>
    <keyword evidence="11" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="11">
        <location>
            <begin position="7"/>
            <end position="111"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008045"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011716"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00016304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023186"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00025077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00033461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00307"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR02338"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95973.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="113" mass="13433" checksum="ECC26E68A3BA3DD9" modified="2013-06-26" version="1">MEIPPQLQAQLMQLQQLQQQLQMIILQKQNVEIELNETKKALEELDKSDVDYVYKLVGGLFIKRNKEDVKKELNEKIETLELRLKTLEKQEEKLQSKLKEIQARLQRFANQAQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1A9</accession>
    <name>N6V1A9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Periplasmic binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04283</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96058.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1A9"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.838"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1980">
        <property type="entry name" value="Nitrogenase molybdenum iron protein domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96058.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="51" mass="5950" checksum="76EABEA01E572EB8" modified="2013-06-26" version="1">MGKVLYPERFKDINPEEKADNIYIFLVGKPVYKIMAEHYGGFKKITLEDLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1C5</accession>
    <name>N6V1C5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5 8 9">Riboflavin synthase</fullName>
            <ecNumber evidence="4 8 9">2.5.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_04308</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 9">
            <text>2 6,7-dimethyl-8-(1-D-ribityl)lumazine + H(+) = 5-amino-6-(D-ribitylamino)uracil + riboflavin</text>
            <dbReference type="Rhea" id="RHEA:20772"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15934"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58201"/>
            <dbReference type="EC" id="2.5.1.9"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor biosynthesis; riboflavin biosynthesis; riboflavin from 2-hydroxy-3-oxobutyl phosphate and 5-amino-6-(D-ribitylamino)uracil: step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the DMRL synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.9" evidence="4 8 9"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96063.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1C5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00662"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.844"/>
    <dbReference type="UniPathway" id="UPA00275">
        <property type="reaction ID" value="UER00405"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009349">
        <property type="term" value="C:riboflavin synthase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004746">
        <property type="term" value="F:riboflavin synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd09210">
        <property type="entry name" value="Riboflavin_synthase_archaeal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.960">
        <property type="entry name" value="Lumazine/riboflavin synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002180">
        <property type="entry name" value="LS/RS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036467">
        <property type="entry name" value="LS/RS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006399">
        <property type="entry name" value="Ribfl_synth_arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01506">
        <property type="entry name" value="ribC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00885">
        <property type="entry name" value="DMRL_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF015750">
        <property type="entry name" value="Ribfl_synth_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52121">
        <property type="entry name" value="Lumazine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 9" id="KW-0686">Riboflavin biosynthesis</keyword>
    <keyword evidence="7 9 10" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007424"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012827"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013950"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022619"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR01506"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PIRNR" id="PIRNR015750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96063.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="155" mass="17360" checksum="CA63F458A73F8C0D" modified="2013-06-26" version="1">MKKVGIVDTTFARVDMASAAIKKLKELSPNIKIIRKTVPGIKDLPVACKKLLEEEGCDIVMALGMPGKTEKDKVCAHEASLGIIMAQLMTNKHIIEVFVHEDEAKDEKELDWLAKRRAEEHAENVYYLLFKPEKLTKMAGKGLRQGFEDVGPARE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1K0</accession>
    <name>N6V1K0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Amino acid-binding ACT protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03813</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96153.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591355.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1K0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00394"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.747"/>
    <dbReference type="OrthoDB" id="60296at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04886">
        <property type="entry name" value="ACT_ThrD-II-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044561">
        <property type="entry name" value="ACT_ThrD-II-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="1">
        <location>
            <begin position="3"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96153.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="158" mass="17993" checksum="68720E2F4C9952C1" modified="2013-06-26" version="1">MITIDLELKDRPGELLKILTPIAKAGANIISIIHSREEKRGGKVPVRIVIDAEEEKVEKIVEELKKIATLKKVDGRDKKVYLDIVVIGHVVDTDVRDTIDRINEIGLVEDLDLIMPHPEKESSAIMRITVDKDKIDDLFYLFEKLEKEKGLLFIKSII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1P2</accession>
    <name>N6V1P2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="15">Pyruvate carboxylase subunit A</fullName>
            <ecNumber evidence="5">6.4.1.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="16">Pyruvic carboxylase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">J422_03648</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>hydrogencarbonate + pyruvate + ATP = oxaloacetate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20844"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16452"/>
            <dbReference type="ChEBI" id="CHEBI:17544"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.4.1.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="4">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="14">Heterooctamer of four A and four B subunits.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.4.1.1" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96208.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591266.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1P2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01375"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.712"/>
    <dbReference type="OrthoDB" id="31083at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004736">
        <property type="term" value="F:pyruvate carboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000003">
        <property type="entry name" value="acetyl-CoA carboxylase isoform X1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000028">
        <property type="entry name" value="Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.20:FF:000010">
        <property type="entry name" value="Propionyl-CoA carboxylase subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051602">
        <property type="entry name" value="ACC_Biotin_Carboxylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004549">
        <property type="entry name" value="Acetyl_CoA_COase_biotin_COase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005481">
        <property type="entry name" value="BC-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011764">
        <property type="entry name" value="Biotin_carboxylation_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005482">
        <property type="entry name" value="Biotin_COase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005479">
        <property type="entry name" value="CPAse_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00514">
        <property type="entry name" value="accC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006367">
        <property type="entry name" value="PRK08591.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006406">
        <property type="entry name" value="PRK08654.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48095:SF2">
        <property type="entry name" value="BIOTIN CARBOXYLASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR48095">
        <property type="entry name" value="PYRUVATE CARBOXYLASE SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02785">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00289">
        <property type="entry name" value="Biotin_carb_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02786">
        <property type="entry name" value="CPSase_L_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00878">
        <property type="entry name" value="Biotin_carb_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01209">
        <property type="entry name" value="GARS_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50979">
        <property type="entry name" value="BC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00866">
        <property type="entry name" value="CPSASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00867">
        <property type="entry name" value="CPSASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="10 17" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="6" id="KW-0312">Gluconeogenesis</keyword>
    <keyword evidence="7 20" id="KW-0436">Ligase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 17" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12 20" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Biotin carboxylation" evidence="19">
        <location>
            <begin position="1"/>
            <end position="445"/>
        </location>
    </feature>
    <feature type="domain" description="ATP-grasp" evidence="18">
        <location>
            <begin position="120"/>
            <end position="316"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00002380"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013057"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022432"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023317"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00049382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00064342"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00073540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00079226"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="18">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS50979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ENN96208.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="498" mass="55268" checksum="37890DD22686B26F" modified="2013-06-26" version="1">MFKKILIANRGEIAIRIIRACWEMGIKTVAIYSEADKKSLHATLADEAYCVGEAPAIKSYLNMDAILKVAKKADVDAIHPGYGFLAENAEFAKRVIEEGFVFIGPNPEAIKSMGSKINAKKIMKKAGVPLLPGSDGAVEDIDEAIKIAESIGYPVAVKASSGGGGMGISVAYNKEELIDVIESARNIAKSAFGDPTVFIEKYLENPRHIEIQLLADKHGNIIHLGDRECSIQRRHQKLIEEAPSPIMTEELRERMGEAAIRAGKAINYDSVGTVEFLYQDGQFYFLEMNTRIQVEHTVTEQITGIDLVKAMIRVAYGEELWLDQEDVKFNGHAIECRINAEDPLNDFAPCPGKIKLYRSPGGPGVRLDSGVCGGAEIPPYYDPLIAKLITYGNSREEAIVRMRRALREYVIVGVDTNIPFHRAVLEEENFLKGNISTKYVERNMPKLKDKILKYGLELRDLYSAVSEKVFEKNKAIAAAVGGLNMYISQIIKKNDERK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1P7</accession>
    <name>N6V1P7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">CoB/CoM heterodisulfide reductase, subunit B</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_03673</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Part of a complex that catalyzes the reversible reduction of CoM-S-S-CoB to the thiol-coenzymes H-S-CoM (coenzyme M) and H-S-CoB (coenzyme B).</text>
    </comment>
    <comment type="pathway">
        <text evidence="2">Cofactor metabolism; coenzyme M-coenzyme B heterodisulfide reduction; coenzyme B and coenzyme M from coenzyme M-coenzyme B heterodisulfide: step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HdrB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96213.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591276.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1P7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01370"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.717"/>
    <dbReference type="OrthoDB" id="144689at2157"/>
    <dbReference type="UniPathway" id="UPA00647">
        <property type="reaction ID" value="UER00700"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051912">
        <property type="term" value="F:CoB--CoM heterodisulfide reductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1050.140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11810">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017678">
        <property type="entry name" value="CoB/CoM_hetero-S_Rdtase_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004017">
        <property type="entry name" value="Cys_rich_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051278">
        <property type="entry name" value="HdrB/HdrD_reductase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03288">
        <property type="entry name" value="CoB_CoM_SS_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42947">
        <property type="entry name" value="COB--COM HETERODISULFIDE REDUCTASE SUBUNIT B 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42947:SF1">
        <property type="entry name" value="COB--COM HETERODISULFIDE REDUCTASE SUBUNIT B 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02754">
        <property type="entry name" value="CCG"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Cysteine-rich" evidence="6">
        <location>
            <begin position="3"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="domain" description="Cysteine-rich" evidence="6">
        <location>
            <begin position="148"/>
            <end position="240"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003406"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00010431"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF02754"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96213.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="295" mass="32848" checksum="26977665DF540438" modified="2013-06-26" version="1">MKYAFFLGCIMPHRYPGIEKATFKVMKALGVDLEYMPGASCCPAPGVFGSFDLKTWLTIAARNLAIAEEMGCDIVTVCNGCYGTLYEAAHILHENKEALDFVNEKLSKIGKEYKGNVKVRHFAELIYNDIGVDKIKNCVVRPLDKLNVAIHYGCHFLKPSEIKKLDSSERPRILEDIVSATGAKPIYYKDYQMCCGAGGGVRARFLETALKMTREKIINMLDAKADCTVNVCPFCHLQFDRGQKEIEKIFGDKFNLPVLHLSQLLGLAIGMSPEDLAVNVHAIPVDPVLKKLEII</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1T2</accession>
    <name>N6V1T2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Phosphate-binding protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_03258</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the PstS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96248.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590944.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1T2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01039"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.638"/>
    <dbReference type="OrthoDB" id="10255at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042301">
        <property type="term" value="F:phosphate ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035435">
        <property type="term" value="P:phosphate ion transmembrane transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd13565">
        <property type="entry name" value="PBP2_PstS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005673">
        <property type="entry name" value="ABC_phos-bd_PstS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024370">
        <property type="entry name" value="PBP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050962">
        <property type="entry name" value="Phosphate-bind_PstS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00975">
        <property type="entry name" value="3a0107s03"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42996">
        <property type="entry name" value="PHOSPHATE-BINDING PROTEIN PSTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42996:SF1">
        <property type="entry name" value="PHOSPHATE-BINDING PROTEIN PSTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12849">
        <property type="entry name" value="PBP_like_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002756">
        <property type="entry name" value="PstS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51257">
        <property type="entry name" value="PROKAR_LIPOPROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0592">Phosphate transport</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="PBP" evidence="5">
        <location>
            <begin position="28"/>
            <end position="334"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008725"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022592"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="PIRNR" id="PIRNR002756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF12849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96248.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="366" mass="40306" checksum="D32F943BCD567642" modified="2013-06-26" version="1">MRKILIILLTILISTSFLAGCVNEKSSQKETQKIITIRTTGATFPKYQIEKWIADFQKEYPNIKVEYEGGGSGHGQDAFLKGLTHIGRSDPPVTEKMWKKFLSTGDQPLQFPEIVGAVVVTYNVPEIGKATLNLTPEVIAKIFKGDIKYWDDPAIKELNPGLNLPHKEIVTIHRSDASGTTKIFTTYLNLATNGLWPSDYVGKNWPSNLGGIGGKGNPGVIAILEKTPYSIAYTELSYTIKNNLPVAAIKNKAGNFVTPTDETIKAAVAAVKANIPDPTEGYKEDLKQLLNAPGKNAYPIVAFTHFLVWENKNKKHYSTEEAKAVKTFVKWVLTEGQKPEHLAEGYVGLPPEVAQIGLKAIDMVKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2H2</accession>
    <name>N6V2H2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Methyl-coenzyme M reductase operon protein D</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02200</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96463.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590266.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2H2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01188"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.432"/>
    <dbReference type="OrthoDB" id="109281at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003901">
        <property type="entry name" value="Me_CoM_Rdtase_D"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03260">
        <property type="entry name" value="met_CoM_red_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02505">
        <property type="entry name" value="MCR_D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005636">
        <property type="entry name" value="McrD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96463.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="159" mass="18273" checksum="C325B4DF62FD3233" modified="2013-06-26" version="1">MEIEIFPHRYLKASTTEKFLNKLYDLKTVERVVIHGQPLPKVVTYGPARGLPVNHSERKIINVKGVPIELTVMAGRFIVTLSDDSELEKIKKICEEMFPFGYDIRVGKFLKDRPTVTDYLKYGEDGVYLINEMDRRLIGMVDPRSKLSSSVAVVEKREE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2J1</accession>
    <name>N6V2J1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Hef nuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_02275</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96478.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590287.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2J1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01203"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.447"/>
    <dbReference type="OrthoDB" id="9764at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004386">
        <property type="term" value="F:helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004518">
        <property type="term" value="F:nuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd12089">
        <property type="entry name" value="Hef_ID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20075">
        <property type="entry name" value="XPF_nuclease_XPF_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10130">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1320.20">
        <property type="entry name" value="hef helicase domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011545">
        <property type="entry name" value="DEAD/DEAH_box_helicase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006166">
        <property type="entry name" value="ERCC4_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041755">
        <property type="entry name" value="Hef_ID"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011335">
        <property type="entry name" value="Restrct_endonuc-II-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010994">
        <property type="entry name" value="RuvA_2-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010337">
        <property type="entry name" value="PRK13766.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025">
        <property type="entry name" value="FANCONI ANEMIA GROUP M FANCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14025:SF20">
        <property type="entry name" value="FANCONI ANEMIA GROUP M PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00270">
        <property type="entry name" value="DEAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02732">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21210">
        <property type="entry name" value="RNA_helicase_helical"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00891">
        <property type="entry name" value="ERCC4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52980">
        <property type="entry name" value="Restriction endonuclease-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47781">
        <property type="entry name" value="RuvA domain 2-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0347">Helicase</keyword>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="6">
        <location>
            <begin position="22"/>
            <end position="186"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="7">
        <location>
            <begin position="337"/>
            <end position="507"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="481"/>
            <end position="517"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96478.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="739" mass="84998" checksum="68F0712AD08356C9" modified="2013-06-26" version="1">MYITHPLIKPNKLEARLYQQIIAAMALNKNTLVVLPTGLGKTAIAILTIAGMLTKKDGKVLFLAPTRPLVEQHYRTLKEILNIDEDKIIALTGKISPDKREKLYRQGKIFVATPQVIENDIISKRINIDEFVLLIADEAHHTVGNHAYSFVAKHFKDKCRVLALTASPGSDIEKILEICENLGIEHIEIRTGDEPDVRPYLLTVKIIPVKIDLPLEFRKALKLLDEAIKDRLKFLKEKGVIDSVRITKTDLIELNNKLFSYDDEVKYELIRICSEALKLMHAKELLETQGKSIFLNYMEKLQNQRTKSAKNIFEDNRVREAILLLKNTDVEHPKLNKTVELVKKILNNNKNERIIVFTQYRNTVEKIVNLLKQEGINAIRFIGQASRDGKGMSQKEQVEAIERFKNEGGVLVSTSVSEEGIDIPTVNYIIFYEPVPSEIRFIQRRGRAMRGEGGRVYILIAKGTADEAYYWSAIYKEREMKRLLKNLCEAVNRRLKKKLEEQKKEEKKEEKKEITLLDFINKDNINKEEKEESKVKIIVDVREKTMAKLLHDRADVELTTLEVGDYVLSDRVVVERKTAEDFVNSIIDKRLFNQLKDLKKVEKPILIIEGENFSRIHENALKGAILSIILDFGIPIIFTKDENDTADILIRIAEKEQIKEKRPVMIRHGKTAMSLKERQKFIVQSLPDVGGALAERLLKHFKTVEKVFTAKEEELLKVEGVGKERAKKIREVLTAEYKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2X8</accession>
    <name>N6V2X8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Replication factor A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_01435</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000010">
        <property type="protein sequence ID" value="ENN96583.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589948.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2X8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01022"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.281"/>
    <dbReference type="OrthoDB" id="6262at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000724">
        <property type="term" value="P:double-strand break repair via homologous recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010212">
        <property type="term" value="P:response to ionizing radiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04491">
        <property type="entry name" value="SoSSB_OBF"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004365">
        <property type="entry name" value="NA-bd_OB_tRNA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031657">
        <property type="entry name" value="REPA_OB_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051231">
        <property type="entry name" value="SOSS-B"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356">
        <property type="entry name" value="OB FOLD NUCLEIC ACID BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13356:SF0">
        <property type="entry name" value="SOSS COMPLEX SUBUNIT B HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16900">
        <property type="entry name" value="REPA_OB_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01336">
        <property type="entry name" value="tRNA_anti-codon"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="5"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Replication protein A OB" evidence="3">
        <location>
            <begin position="63"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="2">
        <location>
            <begin position="190"/>
            <end position="250"/>
        </location>
    </feature>
    <feature type="domain" description="OB" evidence="2">
        <location>
            <begin position="274"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01336"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF16900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96583.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="604" mass="69968" checksum="BE127D8060FF35AB" modified="2013-06-26" version="1">MDLDEYKKLKKKVMEKLKIDEQTLDKMIDEKYNEYGGILLKTGILYLIAKEHGIDLKEEEFLIKDIKEGMIVDIIGVVRKVYDVKEYDGKKVRKIIIADKSGSITVSLWNELADIEIKEGDVIRLKRVKARKWNKRLELTSLPETEIEILEDYKIELPEYKISDLIPGMVVELEGEVVLAFPVKEFEKDNKKLKLKSFNLRDETGVIRVVLWNELTEIDVRRGDFVKVKGVVKEGYYGGLEIVADEVEIIERGEKIESEVVNIKDLHNYENEIVSVRGKVLALGNKKIVNIDGKRNKVQEILLDDGSGKVKITFWKGLINLLENIKEGDIIKVTNCKVKSYIDREGNIRYDLIATIDSEVIKEGESEYKIKFVKIKDIVNKEVDWEDINLIAIVVDITEHEFDGRKVKNILLEDDTGRIRLSIWEDLDIDIEEGDFIKVYHAYAKERDDYIDLYLGRFGRIEKSDERVIKRKFIADLLPGDKVEIRGTIVEVLNEEPFIYMCPNCRKIITEEVCEDCKVEAEKVLRLNVILDDGTGVITCRLYNKKVERLLKIFNVLIENFKEKLLGEEIIFYGNVKDQFIVRALKPVNVDEEIKILSGIKDES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6V352</accession>
    <name>N6V352_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">GTP-dependent dephospho-CoA kinase</fullName>
            <ecNumber evidence="6">2.7.1.237</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Dephospho-coenzyme A kinase</fullName>
            <shortName evidence="6">DPCK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_00891</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the GTP-dependent phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A (CoA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>3'-dephospho-CoA + GTP = GDP + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:61156"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57328"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="2.7.1.237"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the GTP-dependent DPCK family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.237" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96683.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589848.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V352"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00596"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.175"/>
    <dbReference type="OrthoDB" id="15447at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00590">
        <property type="entry name" value="Dephospho_CoA_kinase_GTP_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007164">
        <property type="entry name" value="GTP-dep_dephospho-CoA_kin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732:SF1">
        <property type="entry name" value="GTP-DEPENDENT DEPHOSPHO-COA KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40732">
        <property type="entry name" value="UPF0218 PROTEIN TK1697"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04019">
        <property type="entry name" value="DUF359"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006533">
        <property type="entry name" value="UCP006533"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0173">Coenzyme A biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 6" id="KW-0418">Kinase</keyword>
    <keyword evidence="2 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 6" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="6">
        <location>
            <position position="35"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="36"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="37"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="56"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022993"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00590"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96683.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="158" mass="18168" checksum="98DA38E7CEEB1A3D" modified="2013-06-26" version="1">MLKLPNYLREELRKPFGKVYKTIPNINGDIVTVGDIVTKTLIEKNIIPKLSIIDKKTKRNIPVEINHKFKKVFKVKNPPGYISDEAIESIKYLSKLNDRDVALIVDGEEDLLTILVIKYFPIGTYVLYGLPDKGMVVLKINSKLKQKIEEILKKFEKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3I8</accession>
    <name>N6V3I8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 10 11">Ribulose-phosphate 3-epimerase</fullName>
            <ecNumber evidence="7 10 11">5.1.3.1</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpe</name>
        <name evidence="16" type="ORF">J422_00200</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the reversible epimerization of D-ribulose 5-phosphate to D-xylulose 5-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 12">
            <text>D-ribulose 5-phosphate = D-xylulose 5-phosphate</text>
            <dbReference type="Rhea" id="RHEA:13677"/>
            <dbReference type="ChEBI" id="CHEBI:57737"/>
            <dbReference type="ChEBI" id="CHEBI:58121"/>
            <dbReference type="EC" id="5.1.3.1"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10 14">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
        <text evidence="10 14">Binds 1 divalent metal cation per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Carbohydrate degradation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6 10 12">Belongs to the ribulose-phosphate 3-epimerase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.1.3.1" evidence="7 10 11"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96823.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589729.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3I8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01128"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.40"/>
    <dbReference type="OrthoDB" id="53073at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004750">
        <property type="term" value="F:D-ribulose-phosphate 3-epimerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019323">
        <property type="term" value="P:pentose catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00429">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000004">
        <property type="entry name" value="Ribulose-phosphate 3-epimerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02227">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026019">
        <property type="entry name" value="Ribul_P_3_epim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000056">
        <property type="entry name" value="Ribul_P_3_epim-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011060">
        <property type="entry name" value="RibuloseP-bd_barrel"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004076">
        <property type="entry name" value="PRK05581.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01163">
        <property type="entry name" value="rpe"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11749">
        <property type="entry name" value="RIBULOSE-5-PHOSPHATE-3-EPIMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00834">
        <property type="entry name" value="Ribul_P_3_epim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001461">
        <property type="entry name" value="RPE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51366">
        <property type="entry name" value="Ribulose-phoshate binding barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01085">
        <property type="entry name" value="RIBUL_P_3_EPIMER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01086">
        <property type="entry name" value="RIBUL_P_3_EPIMER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10 12" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="14" id="KW-0170">Cobalt</keyword>
    <keyword evidence="9 10 12" id="KW-0413">Isomerase</keyword>
    <keyword evidence="14" id="KW-0464">Manganese</keyword>
    <keyword evidence="8 10 14" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="14" id="KW-0862">Zinc</keyword>
    <feature type="active site" description="Proton acceptor" evidence="10 13">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="10 13">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10 15">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 14">
        <location>
            <position position="32"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 14">
        <location>
            <position position="34"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 14">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 15">
        <location>
            <position position="65"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 15">
        <location>
            <begin position="139"/>
            <end position="142"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="172"/>
            <end position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10 14">
        <location>
            <position position="172"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="15">
        <location>
            <position position="174"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001782"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00001947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00009541"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00013188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02227"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="NCBIfam" id="TIGR01163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="PIRNR" id="PIRNR001461"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR001461-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN96823.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="228" mass="25802" checksum="32744305CC2C5EBC" modified="2013-06-26" version="1">MIKIGASILSADFRFLKHEIEKVENAGVDFIHVDMMDGHFVPNISMGIGIANYVKKITNLPVDVHLMVENADIFLNEFKDMDFITFHIEAVRFPFRMINKIRELGAKPIVAINPATPLDMIEYILEEVYGVLLMTVEPGFSGQKFIMPMLKKIKRLKNLIEENNLETKILVDGGINRETAYLAVKSGADWLIASSAIFRNPDVNRAVKELRESALKALGERNEINRSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3J7</accession>
    <name>N6V3J7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">4Fe-4S ferredoxin iron-sulfur binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_00260</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96833.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589739.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3J7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01117"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.52"/>
    <dbReference type="OrthoDB" id="2837at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF1">
        <property type="entry name" value="FERREDOXIN III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13237">
        <property type="entry name" value="Fer4_10"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="4"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="20"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="70"/>
            <end position="99"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="125"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="159"/>
            <end position="182"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96833.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="182" mass="20645" checksum="D3AB66B12AB311DA" modified="2013-06-26" version="1">MKISSLIKCLFLNVDKKYSKTKELDINECIGCGLCTKHCPTEAIKIFKFRNIICEHCGVCLDICDAIIGNRFSIDESKCKKCGICLLFCSIPVIKNNVPKPKTPIILEYKCNECGLCSCEAIDLKNKKIDENKCKFCLECVKKCPLKAIITPEEYVKSMFIKVDVESCIFCRMCEEVCPIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VNY5</accession>
    <name>N6VNY5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Peptide chain release factor 1</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_07207</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000080">
        <property type="protein sequence ID" value="ENN95555.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VNY5"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003747">
        <property type="term" value="F:translation release factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.960.10">
        <property type="entry name" value="eRF1 domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024049">
        <property type="entry name" value="eRF1_1_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004403">
        <property type="entry name" value="Peptide_chain-rel_eRF1/aRF1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10113">
        <property type="entry name" value="PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03463">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55481">
        <property type="entry name" value="N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95555.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="102" mass="11431" checksum="DBFA7E4A241BB791" modified="2013-06-26" version="1" fragment="single">MSNDKYTFKKLIKELKSKKGKGTELISLYIPAGRRISDVAQHLREEMSQAANIKSKSTRKNVQSAIEAIMQRLKLLKEPLEKGVVIFAGMVPRSGPGTEKME</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP65</accession>
    <name>N6VP65_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">phosphoserine phosphatase</fullName>
            <ecNumber evidence="4">3.1.3.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">O-phosphoserine phosphohydrolase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_06668</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="2">Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 3/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily. SerB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.3" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000057">
        <property type="protein sequence ID" value="ENN95650.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593901.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP65"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00717"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1298"/>
    <dbReference type="OrthoDB" id="10041at2157"/>
    <dbReference type="UniPathway" id="UPA00135">
        <property type="reaction ID" value="UER00198"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036424">
        <property type="term" value="F:L-phosphoserine phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006564">
        <property type="term" value="P:L-serine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050582">
        <property type="entry name" value="HAD-like_SerB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004469">
        <property type="entry name" value="PSP"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01488">
        <property type="entry name" value="HAD-SF-IB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00338">
        <property type="entry name" value="serB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43344:SF2">
        <property type="entry name" value="PHOSPHOSERINE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00702">
        <property type="entry name" value="Hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01137">
        <property type="entry name" value="C1.6.1:_Phosphoserine_Phosphat"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01136">
        <property type="entry name" value="C1.6:_Phosphoserine_Phosphatas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00029">
        <property type="entry name" value="phosphoserine_phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0718">Serine biosynthesis</keyword>
    <feature type="active site" description="Nucleophile" evidence="11">
        <location>
            <position position="8"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="11">
        <location>
            <position position="10"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009184"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00012640"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00031693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PIRSR" id="PIRSR604469-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95650.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="207" mass="23392" checksum="0A9AF791BE9F9AAE" modified="2013-06-26" version="1">MKKLVVFDFDSTLIENETIDELAKEANKEEEIKEITKKAMNGEIDFKEALKKRVSLLKGLPIEKVENAINRLKLTNGAEETIKELKNRGYKVAVVSGGFDIAINKFKYLDFDYIFANKLIFKDNKLTGEVIGKVLDNTSKGKIIEEVAKKENIPLKNIIVVADGANDVSMFEKAGLKIAFCAKPILKKKADVIIEKRDLREVLKFIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPE3</accession>
    <name>N6VPE3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Triphosphoribosyl-dephospho-CoA protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06100</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95750.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593242.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPE3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00539"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1188"/>
    <dbReference type="OrthoDB" id="85890at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046917">
        <property type="term" value="F:triphosphoribosyl-dephospho-CoA synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.4200.10">
        <property type="entry name" value="Triphosphoribosyl-dephospho-CoA protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002736">
        <property type="entry name" value="CitG"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42280">
        <property type="entry name" value="CITG FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42280:SF1">
        <property type="entry name" value="CITG FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01874">
        <property type="entry name" value="CitG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="101"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95750.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="293" mass="33610" checksum="118B9108409BF686" modified="2013-06-26" version="1">MDSFDIMKASQIACCLEVSSLKPGNVHRNRDYKDIKYHHFITSGIAFGNIIYLASKDNKNVGYYIKRAVIESKKWSPSNANLGIIILHIPISMASGMLNSFNKNELKRKIKNITKNTTVDDAINFCEAISIIKPNINKPHDGPDITKDDSKREIIERNLTLYDLFKISYSWDNISKEWVDGFNISFEGYEKLKRYYEELEDINLSITKTYLHILSKYPDSLIARKNNIEVAENVSKMAKEVLDNFSLENVIEFDNYLARYGNKLNPGTTADIIASSLMIFLLEKIDKNDTILK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPH4</accession>
    <name>N6VPH4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">NAD-dependent epimerase/dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05893</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000046">
        <property type="protein sequence ID" value="ENN95790.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592961.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPH4"/>
    <dbReference type="SMR" id="N6VPH4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00993"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1149"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="4"/>
            <end position="331"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95790.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="345" mass="40289" checksum="F60A55CAE17E15AE" modified="2013-06-26" version="1">MKILITGGLGFLGSNLASESINREYETIIMDNFFREGTTLNYNWLKEQGEFIFENKDIRIWYDVEEMIKKYKPDVVFHTAGQVAMTTSLKNPRLDFEVNALGTFNLLEAIRKYSPETIVLYSSTNKVYGDLEYIKYIETETRYIAPDYPNGFDESLKLDFHTPYGCSKGTADQYLLDYHRIFGIKTVVFRHSSMYGGRQFATYDQGWIGWFIREALRIKREEIEEVKIHGNGKQVRDVLHADDVINLYFKTVDNIEKCAGEAFNIGGGMENSLSLLELFRFLEDELDIEIKINKKPWRISDQKIFVADITKINKYTGWKPKISKEEGLKRMIKWCEEILGERRVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPP5</accession>
    <name>N6VPP5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Endonuclease III</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_05479</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000041">
        <property type="protein sequence ID" value="ENN95870.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592556.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPP5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01404"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1070"/>
    <dbReference type="OrthoDB" id="19248at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006284">
        <property type="term" value="P:base-excision repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00056">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.1670.10">
        <property type="entry name" value="Helix-hairpin-Helix base-excision DNA repair enzymes (C-terminal)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.340.30">
        <property type="entry name" value="Hypothetical protein, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011257">
        <property type="entry name" value="DNA_glycosylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003651">
        <property type="entry name" value="Endonuclease3_FeS-loop_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003265">
        <property type="entry name" value="HhH-GPD_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023170">
        <property type="entry name" value="HhH_base_excis_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359">
        <property type="entry name" value="A/G-SPECIFIC ADENINE GLYCOSYLASE/ENDONUCLEASE III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10359:SF19">
        <property type="entry name" value="DNA REPAIR GLYCOSYLASE MJ1434-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00730">
        <property type="entry name" value="HhH-GPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001435">
        <property type="entry name" value="Nth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00478">
        <property type="entry name" value="ENDO3c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00525">
        <property type="entry name" value="FES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48150">
        <property type="entry name" value="DNA-glycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="7" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="5" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HhH-GPD" evidence="6">
        <location>
            <begin position="33"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95870.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="213" mass="25212" checksum="E66AA74362877DA5" modified="2013-06-26" version="1">MIYKIYKLLLNHYGYQNWWPAESRYEVVVGVILTQNTSWKNVEKAIKNLKAKNLLDEEKILNISMNELKELIKPAGFYNIKSERLKEITSFIVNNYGSTENMYLSKEPMKVLREKLLNVKGIGKESADSILLYALDRPSFVVDTYTKRLFNRLGILNSEDYEYIKRIFEENIPKDLKVYKEYHALIVEHCKTVCKKKPKCNKCFLKDICLFRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPX1</accession>
    <name>N6VPX1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">DNA polymerase</fullName>
            <ecNumber evidence="8">2.7.7.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_04865</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 12">
            <text>DNA(n) + a 2'-deoxyribonucleoside 5'-triphosphate = DNA(n+1) + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:22508"/>
            <dbReference type="Rhea" id="RHEA-COMP:17339"/>
            <dbReference type="Rhea" id="RHEA-COMP:17340"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:61560"/>
            <dbReference type="ChEBI" id="CHEBI:173112"/>
            <dbReference type="EC" id="2.7.7.7"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the DNA polymerase type-B family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.7" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95945.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPX1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00517"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.953"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003887">
        <property type="term" value="F:DNA-directed DNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.132.60:FF:000013">
        <property type="entry name" value="DNA polymerase Pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.60">
        <property type="entry name" value="DNA polymerase family B, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.342.10">
        <property type="entry name" value="DNA Polymerase, chain B, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.690">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1600.10">
        <property type="entry name" value="Palm domain of DNA polymerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006172">
        <property type="entry name" value="DNA-dir_DNA_pol_B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017964">
        <property type="entry name" value="DNA-dir_DNA_pol_B_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006134">
        <property type="entry name" value="DNA-dir_DNA_pol_B_multi_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043502">
        <property type="entry name" value="DNA/RNA_pol_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042087">
        <property type="entry name" value="DNA_pol_B_thumb"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023211">
        <property type="entry name" value="DNA_pol_palm_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050240">
        <property type="entry name" value="DNA_pol_type-B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00592">
        <property type="entry name" value="pol2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322">
        <property type="entry name" value="DNA POLYMERASE CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10322:SF23">
        <property type="entry name" value="DNA POLYMERASE DELTA CATALYTIC SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00136">
        <property type="entry name" value="DNA_pol_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00106">
        <property type="entry name" value="DNAPOLB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00486">
        <property type="entry name" value="POLBc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56672">
        <property type="entry name" value="DNA/RNA polymerases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00116">
        <property type="entry name" value="DNA_POLYMERASE_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8" id="KW-0235">DNA replication</keyword>
    <keyword evidence="6 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5 8" id="KW-0239">DNA-directed DNA polymerase</keyword>
    <keyword evidence="3 8" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="DNA-directed DNA polymerase family B multifunctional" evidence="9">
        <location>
            <begin position="102"/>
            <end position="493"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00049244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000442"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95945.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="516" mass="60635" checksum="56F47CAC8FE85FB8" modified="2013-06-26" version="1">MVINHTSQEVHIDLYPIARRLLKLTKYSLEDVVYALFGIEKLKIGHENIRLFWEDKDKILIEYSLQDAKYTYKLGEYFLPLEVMFSRIVNQNIFSITRMTSGQMVEYLLMKMAYKENMIVPNKPDEEEYKRRVNESYEGGYVKAEKGMFNDIVYFDFRSLYPSIIITYNISPDTIDCDCCNGEKILNHHFCKKREGLIPKTLKGLIKRRIEVKKRMNEVSDEEKKLLDYEQQSLKILANSHYGYLAYPRARFYSKECAEVVTYLGRKYILMTVEEAEKFGFKVVYIDTDGFYAIYKEKIEKNELIEKAKEFIKYINERLPGNMELEFEGYYKRGIFITKKKYALVDDNNRIIIKGLEFVRRDWANIAKTTQKKVLEALLIEGDIEKAKNIIKEIIKELREGKIKKEDLIIYTQITKDIKEYKTTAPHIELAKRLIREGKKVNVGDVIGYIIVKGAKSISERAKLPEEVDIKDVDINYYIDNQILPPILRVMEAVGVSKNELKKDGKQITLDSFFKT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQA5</accession>
    <name>N6VQA5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transport system permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_04293</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96060.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591748.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQA5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00665"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.841"/>
    <dbReference type="OrthoDB" id="27848at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033214">
        <property type="term" value="P:siderophore-iron import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06550">
        <property type="entry name" value="TM_ABC_iron-siderophores_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.3470.10:FF:000001">
        <property type="entry name" value="Vitamin B12 ABC transporter permease BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472:SF25">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN MJ0876-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="FERRIC ENTEROBACTIN TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="12"/>
            <end position="36"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="65"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="93"/>
            <end position="111"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="117"/>
            <end position="137"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="149"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="192"/>
            <end position="212"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="243"/>
            <end position="274"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="280"/>
            <end position="296"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="308"/>
            <end position="328"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96060.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="331" mass="37027" checksum="339D81CDAF449C47" modified="2013-06-26" version="1">MNYRKYVLKKIYFGIFLILLLFVSFILSLCIGEYYLPINKIISTFLSNNSDITKTVIWNVRLPRVFTAIFVGFSLSLSGLVMQCVLRNPLASPFTLGISQGAMFGVALAVLLNLFSFIYLLPIFAFLGAFISTVIILSLAKLKNLSPEAIVLSGIAIGALFHAGSTFIQYFVDETKLAFIVYWTFGDVGRTSWNEVYMLFLSSILAFLYLMYKRWDFNALQFDDDTAKSLGVEPDRVRLISMLIASFITSVTVAFTGIIGFVGLICPHIIRLIIGEDYRFLIPYSSIFGSIFLLLADDLSRQILSPIILPVGIITSILGAPLLIYLLIKMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQE4</accession>
    <name>N6VQE4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methyl coenzyme M reductase system subunit AtwA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_04453</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96090.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591812.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQE4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00633"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.873"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019700">
        <property type="term" value="P:organic phosphonate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017669">
        <property type="entry name" value="Me_Coenz_M_Rdtase_A2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03269">
        <property type="entry name" value="met_CoM_red_A2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42764:SF2">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42764">
        <property type="entry name" value="PHOSPHONATES UTILIZATION ATP-BINDING PROTEIN PHNK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="4"/>
            <end position="271"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="283"/>
            <end position="531"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96090.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="533" mass="59852" checksum="C34D942FC3CA49C8" modified="2013-06-26" version="1">MILLEVKNVTKRFGGKEVLKNISFTLNEGESLGILGKSGAGKSVLLHMLRGMEGYEPDEGEIIYHVSYCDNCGYVDVPSKDGEKCKKCGSTLKKIEVNFWKDKNYLYNLKKKIAIMLQRTFALYGEKTVIENILEALHQAGYEGKEAIDKALELIKIVKLEHRITHIARDLSGGEKQRVVLARQIAKEPFIFLADEPTGTLDPQTAKLVHSALKELVIKNKISLILTSHWPEVIAELTEKAIWLDKGEIIAEGSSDEIVNKFMEQVGKFEKPKIEVEIKEDIIRLENISKHYCSVERGVIKAVDNVSLTIKENEIFGLVGASGAGKTTLAKIIAGVLPPSKGKYWFRVGDEWVDMTQPGPLGRGRAKRYIGILFQEYALYPHRTILENLTEAIGLELPDEFAFMKATHTLVSVGFSEEEAEEILNKYPNELSVGERHRCALAQVLIKEPRVVILDEPTGTMDPITRNIVANSILKSRIELEQTYIIVSHDMDFVLNVCDRAGLVRNGKLIKVGKPDEIVKLLTEEEKEEMLNQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQP6</accession>
    <name>N6VQP6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">TATA-box-binding protein</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Box A-binding protein</fullName>
            <shortName evidence="7">BAP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">TATA sequence-binding protein</fullName>
            <shortName evidence="7">TBP</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">TATA-box factor</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">tbp</name>
        <name evidence="9" type="ORF">J422_03718</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7 8">General factor that plays a role in the activation of archaeal genes transcribed by RNA polymerase. Binds specifically to the TATA box promoter element which lies close to the position of transcription initiation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the TBP family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96220.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591289.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQP6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01362"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.726"/>
    <dbReference type="OrthoDB" id="350539at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006352">
        <property type="term" value="P:DNA-templated transcription initiation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd04518">
        <property type="entry name" value="TBP_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000007">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.10:FF:000010">
        <property type="entry name" value="TATA-box-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.10">
        <property type="entry name" value="TATA-Binding Protein"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00408">
        <property type="entry name" value="TATA_bind_prot_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000814">
        <property type="entry name" value="TBP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033711">
        <property type="entry name" value="TBP_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030491">
        <property type="entry name" value="TBP_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012295">
        <property type="entry name" value="TBP_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001593">
        <property type="entry name" value="PRK00394.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001594">
        <property type="entry name" value="PRK00394.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10126">
        <property type="entry name" value="TATA-BOX BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00352">
        <property type="entry name" value="TBP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00686">
        <property type="entry name" value="TIFACTORIID"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55945">
        <property type="entry name" value="TATA-box binding protein-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00351">
        <property type="entry name" value="TFIID"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 8" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0677">Repeat</keyword>
    <keyword evidence="5 7 8" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 7 8" id="KW-0805">Transcription regulation</keyword>
    <feature type="repeat" description="1" evidence="7">
        <location>
            <begin position="6"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="7">
        <location>
            <begin position="97"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00025680"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU000523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96220.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="180" mass="19955" checksum="10184FD5B3289693" modified="2013-06-26" version="1">MPDIKIVNVVVSTKIADSIDLEEAALVLENAEYEPEQFPGLVCRLSVPKVALLLFRSGKVNCTGAKSKKEAEIAINKIINELKDAGFDVYENPEVKIQNMVATADLGVEPNLDEIAMYVDDTEYEPEQFPGLVYRLDDPKVVVLIFGSGKVVITGLKKEEDAKRALDKILETIKEVQGYR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQS8</accession>
    <name>N6VQS8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Cobaltochelatase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_03303</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the Mg-chelatase subunit H family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96255.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590957.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQS8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01047"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.647"/>
    <dbReference type="OrthoDB" id="192131at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051116">
        <property type="term" value="F:cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016851">
        <property type="term" value="F:magnesium chelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015995">
        <property type="term" value="P:chlorophyll biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10150">
        <property type="entry name" value="CobN_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011771">
        <property type="entry name" value="BchH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011953">
        <property type="entry name" value="Cobalto_CobN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003672">
        <property type="entry name" value="CobN/Mg_chltase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02025">
        <property type="entry name" value="BchH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02257">
        <property type="entry name" value="cobalto_cobN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119:SF4">
        <property type="entry name" value="AEROBIC COBALTOCHELATASE SUBUNIT COBN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR44119">
        <property type="entry name" value="MAGNESIUM-CHELATASE SUBUNIT CHLH, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02514">
        <property type="entry name" value="CobN-Mg_chel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="CobN/magnesium chelatase" evidence="3">
        <location>
            <begin position="112"/>
            <end position="1179"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="2">
        <location>
            <begin position="599"/>
            <end position="671"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF02514"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96255.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="1198" mass="139896" checksum="38B69BC5E9856D50" modified="2013-06-26" version="1">MVKIAFISTVDNDDLTFEEAYKEIKDIIEFKILDAKCSEEEFKEFLKFVKDANIVFTKLMGGKEAFKYYDELKEFLLKNNIPFLPLPTTSEFYPELERDVTVSEEIKEEVRKYLSYEGVENYKNLLLYLANKFGDCSIPYDKPKPMPWQGIYYKGKCYENLEEYLKLFKDLNKPKIGILFYRNWFISRNIEYVDYLIEIIEKKGGIPIALFSSHLKNEFGSLGIDENFKRFFYYNNKPIIDVLINTTMFTLSMGVKNDITNEPEFLIDLNVPILQGIVSTDYIENWIKGYSINPIDLVIGMAMPEFDGAIIHFPIAGKEKVKDGDVGVPIIKYKAIKDRAEKIVDLAIKYANLRLKNNKEKRIAIILHNYPPRNDKIASAFGLDSPESVVRLLKELKKRGFHVERLYKNGNELINELLKYPTNDKNFITDEIIKKAVGKIKREDYKKWFNSLSEKVKKELIENWGEIPGNVMNFNGELIIPGIINGNIFISVQPPRGFNEDPSAIYHDPKKPPTHYYIAFYKWIKEVFKADAIMHIGKHGSLEWLPGKGAGLSKDCYPDICMELPNIYPYIVNNPGEGTQAKRRGYATIISHLIPPMTISELYGDLEELERMIDEYYETEKEKKEFLKEEIKKKIKELKLDEDLIDKNINANDFEELLREVHNYLQDLKNRQINDGLHILGKPLDGEKLINMIFMIVRYQFEYLKTIAKILNYDWDELNKNKGKNHKIIDKINKIGIEVIKEFIENEFKIEKISVIKVNDELKKILEIIKNIYDNLMRVKEEIDYAVNALHGYYVPPKVAGAPTKDINCLPTGRNFYSCNPQEIPTKAAYEMGKRLAEELLKKYLEEEGKYPERVGLIVWGSPTMRTKGDDVAEFLYLLGVKPVWNKMGRVVDLEIIPLEELKRPRIDVIARVSGFFRDAFPQLLKLMDKAVNMVANLDEPDDMNFVKKHYKEEIKKFKKFYRIFSDKPGTYGAGISNLLESKNWKSLEDLAKVYVEWGGYAYGENTYGEEAKELFKYNLSKIELTVKNEDSQEWDIFEGDDFNSYHGGMIAAVRYYSKKKPKSYVGDTSCGIKIKNLKEEGKEVFRTKILNPKWINGLKRHGYKGASDFSKYIDHIFQWDATSDIVEDWMYEKIAEKYVFDKKMEEFFKENNPYALLNITERLLEAIERGLWKADDEMKEKLRKKYLEIEGMIEERL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQV3</accession>
    <name>N6VQV3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03216</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000019">
        <property type="protein sequence ID" value="ENN96280.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQV3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00320"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.631"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="18"/>
            <end position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96280.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="65" mass="7816" checksum="63D11D580EA0F1BB" modified="2013-06-26" version="1">MEELLYDVLPYISNMEFIKNLIKEAKDINELEKKVKEMLEKEDNITKKTDLKILLEKIEIYKQQG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQW3</accession>
    <name>N6VQW3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">GTPase HflX</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">GTP-binding protein HflX</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">hflX</name>
        <name evidence="10" type="ORF">J422_02974</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">GTPase that associates with the 50S ribosomal subunit and may have a role during protein synthesis or ribosome biogenesis.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="7">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="5">Monomer. Associates with the 50S ribosomal subunit.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
        <text evidence="5">May associate with membranes.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. HflX GTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96290.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQW3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00142"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.583"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01878">
        <property type="entry name" value="HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.250.2860">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.11060">
        <property type="entry name" value="GTPase HflX, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00900">
        <property type="entry name" value="GTPase_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030394">
        <property type="entry name" value="G_HFLX_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032305">
        <property type="entry name" value="GTP-bd_M"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016496">
        <property type="entry name" value="GTPase_HflX"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025121">
        <property type="entry name" value="GTPase_HflX_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042108">
        <property type="entry name" value="GTPase_HflX_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03156">
        <property type="entry name" value="GTP_HflX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229">
        <property type="entry name" value="GTP-BINDING PROTEIN HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10229:SF8">
        <property type="entry name" value="GTPASE HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16360">
        <property type="entry name" value="GTP-bdg_M"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13167">
        <property type="entry name" value="GTP-bdg_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006809">
        <property type="entry name" value="GTP-binding_hflX_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00326">
        <property type="entry name" value="GTP1OBG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51705">
        <property type="entry name" value="G_HFLX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 5 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Hflx-type G" evidence="9">
        <location>
            <begin position="158"/>
            <end position="327"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="8">
        <location>
            <begin position="126"/>
            <end position="153"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="164"/>
            <end position="171"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="171"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="189"/>
            <end position="193"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="191"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="210"/>
            <end position="213"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="277"/>
            <end position="280"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="305"/>
            <end position="307"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00900"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR006809-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96290.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="377" mass="43733" checksum="731F6CAA92C25704" modified="2013-06-26" version="1">MLYEPVKTFVQIRKPDPKYQIGSGLVERIAKEIKENEIDIVVFGNQLSPSQKYNLAKNFKVEVIDKIELVLRIFYKHAKSREALLQVRLAELKYELPRAKEKVRLAKQGEQPGFGGYGDYEVEKYYQKVKREINIIKSKLEKLKKHRENLRKRRGNFDTVGLIGYTNAGKTSLLNALSGDRKEVKNQVFTTLSTTTRRIRGIKRRILITDTVGFMDDLPPFMIEAFLSTIEESANSDLILLVVDASDSLEEIERKLKVNYEVLDKINCKSPIITVFNKVDKITYEKREEILKSLKRYIVNPIFVSAKYNINLDKLKEMILENLNLSIGTVETDNPKLISYLYENTEILEDVLEDNKHIITFRAKEKDVNRILKAVRV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR17</accession>
    <name>N6VR17_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_07192</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000078">
        <property type="protein sequence ID" value="ENN95561.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004595169.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR17"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01009"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1389"/>
    <dbReference type="OrthoDB" id="64741at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054162">
        <property type="entry name" value="DUF6293_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046260">
        <property type="entry name" value="HFX_2341-like_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19810">
        <property type="entry name" value="HFX_2341_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22665">
        <property type="entry name" value="WHD_DUF6293"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="100"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="domain" description="HFX-2341-like N-terminal" evidence="2">
        <location>
            <begin position="11"/>
            <end position="138"/>
        </location>
    </feature>
    <feature type="domain" description="DUF6293" evidence="3">
        <location>
            <begin position="244"/>
            <end position="321"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF19810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF22665"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95561.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="324" mass="38278" checksum="14652A4F94F9F64E" modified="2013-06-26" version="1">MNKLNKIRGGVHIAVQGYEVDRITEVPIKRRAEKVYLICMPDEEDSERGKAFKNMIKERFEKNKIDYEILEANIFNLDEIVKKIKYAINKERKEFEEVKFYINVSSGSTIGCIAGITCAMILNRENSKIIPYYVIPKKLLDSLSDEECEKLREEYKYLPRSFGVKDVVLIYPFNISLPKEELLIFLKFINRAGDRGLTIKELSILTREEFLDVNLNNEESIMRLIKAVEHIDSDPSRIRIKRYRRTIQKLLEIVEDDKEELNKIVNWRNRTKFSENKTPQSDLVWVNKNVIEKLKEFELIVDEKIGRSRYIKVNEKGKMLLNYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR42</accession>
    <name>N6VR42_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cell wall binding repeat 2-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06992</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000067">
        <property type="protein sequence ID" value="ENN95591.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR42"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00579"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1354"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="80"/>
            <end position="124"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95591.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="199" mass="23239" checksum="5E76E9931A2156EF" modified="2013-06-26" version="1" fragment="single">KKKKKVIIVHGNSEVLINISKDAIVILTNGINLTVNIDNLNPEEVEVVEDPAVNLTNITKKLEKHRIKVRINAIPKHALEKILENRIKEIKRKIKVLKKLGVDTEDLEEELKDLEEKIKEGDYDDAYKLMLKVKNMEMVKVRLYENTHLNLNIEIEKNKKHIEIEIENKHEENETEHEIGNEIKGEKLVKQEKIKIINK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR53</accession>
    <name>N6VR53_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Transcription regulator TrmB C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06957</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000065">
        <property type="protein sequence ID" value="ENN95606.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594637.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000065.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR53"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00858"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1348"/>
    <dbReference type="OrthoDB" id="65805at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021586">
        <property type="entry name" value="Tscrpt_reg_TrmB_C"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11495">
        <property type="entry name" value="Regulator_TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159071">
        <property type="entry name" value="TrmB C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB C-terminal" evidence="1">
        <location>
            <begin position="36"/>
            <end position="112"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF11495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95606.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="239" mass="27435" checksum="CD157C3C9FFC75A6" modified="2013-06-26" version="1">MKRILELLVVLAIIITATAVAYKFINNNKDYEFSGDEMYKCTWIADKILNKGFPLKAHIYGYWVYDKKLFNDTVEVYYARGGILYAIYNNDTVTIGGRLAYKEDIAAEKIELIPIGKYIVVIDINPLEGKDFRDIYERVLKNISFNYIKAYIVGKIAADTETFKPTERIKLDNILFVDEIKGLDVFYIENGLILSGKINLDKLKELDNYIKVNKTSTSKLKLYIVTNDKVSGYKVISLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR66</accession>
    <name>N6VR66_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA (cytosine-5-)-methyltransferase</fullName>
            <ecNumber evidence="1">2.1.1.37</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_06887</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="5">Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.37" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594260.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR66"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01070"/>
    <dbReference type="REBASE" id="68617">
        <property type="entry name" value="M.MviT80ORF6887P"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1336"/>
    <dbReference type="OrthoDB" id="5033at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003886">
        <property type="term" value="F:DNA (cytosine-5-)-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044027">
        <property type="term" value="P:negative regulation of gene expression via chromosomal CpG island methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.120.10">
        <property type="entry name" value="DNA Methylase, subunit A, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050390">
        <property type="entry name" value="C5-Methyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001525">
        <property type="entry name" value="C5_MeTfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629">
        <property type="entry name" value="CYTOSINE-SPECIFIC METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10629:SF52">
        <property type="entry name" value="DNA (CYTOSINE-5)-METHYLTRANSFERASE 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00145">
        <property type="entry name" value="DNA_methylase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00105">
        <property type="entry name" value="C5METTRFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51679">
        <property type="entry name" value="SAM_MT_C5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5 6" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 5" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 5 6" id="KW-0808">Transferase</keyword>
    <feature type="active site" evidence="5">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00011975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01016"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="355" mass="40981" checksum="E63988156F1E2C98" modified="2013-06-26" version="1">MKIIDLFSGCGGFSRGFVEEGFKPVLAIELDEDAAFSYALNFNGIIYEKIGDKFKLKEMKGYVGSYPFRMPLDDESIKWLKYFGTYRDDINPIVLNNDIREIHSLDIERFVKKVDVIIGGPPCEGYTGANPKREKNPHDRLYKDDVGRLVLDFIRIVGDLQPKVFVMENVPAIRDVKGDIIKEFRDVGYEDVYFNILRAEDYGNPSVRRRVFVSNIEIIPEKEKPKTVIEAIGDLMYKGRDVPNHEYAALPRRFWRKVHYLGWGDAFIYFKGASRRLGNYIRLHPLKLAETVMGKRFFIHPYEDRLLTPREQARLMSYPDNHLFVGGIRSCLNQIGESVPPCLSRAIAKVVKENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR91</accession>
    <name>N6VR91_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Aspartate/glutamate/uridylate kinase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02564</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96420.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590473.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR91"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00760"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.502"/>
    <dbReference type="OrthoDB" id="50461at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016301">
        <property type="term" value="F:kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04240">
        <property type="entry name" value="AAK_UC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053666">
        <property type="entry name" value="Furan-3-ylmethyl_P_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011375">
        <property type="entry name" value="MfnE"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040622">
        <property type="entry name" value="MfnE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004857">
        <property type="entry name" value="Kin_aa_kin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0418">Kinase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="1">
        <location>
            <begin position="3"/>
            <end position="163"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96420.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="209" mass="23495" checksum="8BDFF11C1F6193D6" modified="2013-06-26" version="1">MNIIKIGGSLTYYIKPLLESLKSYKREEIIIIPGGGKFADTLREIDKNLSLSPSISHKLAIKCMDILGEVFSDIGGIKAYDNLFDLKREIKKERIAILLPSKLLLSTDISEHSWNVTSDSLTLYISKFLNVKNIIIATDVDGIYDKYPEGKLLNIIKADMIKGLTAVDEYFPTLLKSLNKIAYVVNGKYPERIIDILEGRNTICTKIIP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRC2</accession>
    <name>N6VRC2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 11">Anthranilate phosphoribosyltransferase</fullName>
            <ecNumber evidence="3 11">2.4.2.18</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">trpD</name>
        <name evidence="14" type="ORF">J422_06456</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">Catalyzes the transfer of the phosphoribosyl group of 5-phosphorylribose-1-pyrophosphate (PRPP) to anthranilate to yield N-(5'-phosphoribosyl)-anthranilate (PRA).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>N-(5-phospho-beta-D-ribosyl)anthranilate + diphosphate = 5-phospho-alpha-D-ribose 1-diphosphate + anthranilate</text>
            <dbReference type="Rhea" id="RHEA:11768"/>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <dbReference type="ChEBI" id="CHEBI:18277"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
            <dbReference type="EC" id="2.4.2.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="11">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="11">Binds 2 magnesium ions per monomer.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 11">Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 2/5.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 11">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the anthranilate phosphoribosyltransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.18" evidence="3 11"/>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593546.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRC2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01284"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1257"/>
    <dbReference type="OrthoDB" id="8214at2157"/>
    <dbReference type="UniPathway" id="UPA00035">
        <property type="reaction ID" value="UER00041"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004048">
        <property type="term" value="F:anthranilate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000162">
        <property type="term" value="P:L-tryptophan biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.970.10:FF:000006">
        <property type="entry name" value="Anthranilate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1030.10:FF:000002">
        <property type="entry name" value="Anthranilate phosphoribosyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.10">
        <property type="entry name" value="Transferase, Pyrimidine Nucleoside Phosphorylase, Chain C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00211">
        <property type="entry name" value="TrpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005940">
        <property type="entry name" value="Anthranilate_Pribosyl_Tfrase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01245">
        <property type="entry name" value="trpD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43285:SF2">
        <property type="entry name" value="ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 11" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="10 11" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="5 11 14" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="9 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 11 14" id="KW-0808">Transferase</keyword>
    <keyword evidence="8 11" id="KW-0822">Tryptophan biosynthesis</keyword>
    <feature type="domain" description="Glycosyl transferase family 3 N-terminal" evidence="13">
        <location>
            <begin position="3"/>
            <end position="64"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 3" evidence="12">
        <location>
            <begin position="73"/>
            <end position="321"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="79"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="82"/>
            <end position="83"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="87"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="89"/>
            <end position="92"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="91"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="106"/>
            <end position="114"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="109"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="118"/>
        </location>
        <ligand>
            <name>5-phospho-alpha-D-ribose 1-diphosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58017"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>anthranilate</name>
            <dbReference type="ChEBI" id="CHEBI:16567"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="222"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>2</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="223"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004907"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011948"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF00591"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN95706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="333" mass="36716" checksum="EC06CA74BCC2B179" modified="2013-06-26" version="1">MIDAIKKVVEFKDLSKDEAKKVMQEIMSGKAKETQIAALLTALRMKGETVDEITAFAEVMREYAIKIKPNVEKLIDVCGTGGDNLNTFNISTTVAFVVSVYVPVAKHGNRAVSSKSGSADVLEALGINLNVPIERVKESIEKINIGFLFAPNYHPAMKYALPVRRDIKIRTVFNILGPLTNPANANYQLMGVYDEKLVDKLIYVLKNLNLKGAIVAHGSGMDEITTVGKTKIAEFKDNEIKFYEIEPEQFGFKKAKLEDLMGGSAEENAKIIKNILEGEEGAKRDIVVLNSAFALYICGEAKNVFEGIEKANKAIDSGKALKQLEKLIDFYMG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRE1</accession>
    <name>N6VRE1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Zinc finger ZPR1-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_06214</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ZPR1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95726.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593340.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRE1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00295"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1210"/>
    <dbReference type="OrthoDB" id="14924at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.60.120.1040:FF:000008">
        <property type="entry name" value="Zn finger containing protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.120.1040">
        <property type="entry name" value="ZPR1, A/B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.420">
        <property type="entry name" value="ZPR1, zinc finger domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004457">
        <property type="entry name" value="Znf_ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040141">
        <property type="entry name" value="ZPR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004470">
        <property type="entry name" value="ZPR1-like_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042451">
        <property type="entry name" value="ZPR1_A/B_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056180">
        <property type="entry name" value="ZPR1_jr_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042452">
        <property type="entry name" value="ZPR1_Znf1/2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00340">
        <property type="entry name" value="zpr1_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00310">
        <property type="entry name" value="ZPR1_znf"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10876:SF0">
        <property type="entry name" value="ZINC FINGER PROTEIN ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22794">
        <property type="entry name" value="jr-ZPR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00709">
        <property type="entry name" value="Zpr1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0862">Zinc</keyword>
    <keyword evidence="3" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Zinc finger ZPR1-type" evidence="6">
        <location>
            <begin position="5"/>
            <end position="162"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="5">
        <location>
            <begin position="123"/>
            <end position="150"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95726.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="188" mass="21512" checksum="F5F1AA711D2A7D76" modified="2013-06-26" version="1">MEQRIDCPVCGAKNSFVIITNQFEIPYFGPVLETTMICEKCNFKRSDVFPLEVREPKRYKLTIKDERDLCKRVVRSSSGYIEIPELGVEITPGPLAEGFVSNVEGVLERVNNILDTLYRWADSEEQKKRIEEIKEKIKRIKEGKEKATLILTDPLGHSAIIGEGVEEETLSEEEINKLKEGIIIEKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRE6</accession>
    <name>N6VRE6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">MtN3 and saliva related transmembrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_06239</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95731.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593349.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRE6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00290"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1215"/>
    <dbReference type="OrthoDB" id="11322at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051119">
        <property type="term" value="F:sugar transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1280.290">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006603">
        <property type="entry name" value="PQ-loop_rpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047662">
        <property type="entry name" value="SemiSWEET"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF037968">
        <property type="entry name" value="SemiSWEET_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04193">
        <property type="entry name" value="PQ-loop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="35"/>
            <end position="55"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="61"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95731.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="98" mass="11400" checksum="E3778E90D21FFF1E" modified="2013-06-26" version="1">MDFDIVGYIAGTLTTFASLPQLIKSIRERDLSSISLTFVLTFTTGLSLWLIYGILKMDYPIIVFNILSLMFWIPITYLKIKEEMRKCGMNWVKKLLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRG4</accession>
    <name>N6VRG4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">MCM family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_06105</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the MCM family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593245.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRG4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00540"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1189"/>
    <dbReference type="OrthoDB" id="6747at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042555">
        <property type="term" value="C:MCM complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003697">
        <property type="term" value="F:single-stranded DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017116">
        <property type="term" value="F:single-stranded DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd17706">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031327">
        <property type="entry name" value="MCM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018525">
        <property type="entry name" value="MCM_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001208">
        <property type="entry name" value="MCM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041562">
        <property type="entry name" value="MCM_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033762">
        <property type="entry name" value="MCM_OB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11630:SF66">
        <property type="entry name" value="DNA REPLICATION LICENSING FACTOR MCM4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00493">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17855">
        <property type="entry name" value="MCM_lid"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17207">
        <property type="entry name" value="MCM_OB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01657">
        <property type="entry name" value="MCMFAMILY"/>
    </dbReference>
    <dbReference type="SMART" id="SM00350">
        <property type="entry name" value="MCM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00847">
        <property type="entry name" value="MCM_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50051">
        <property type="entry name" value="MCM_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="5 6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="MCM C-terminal AAA(+) ATPase" evidence="7">
        <location>
            <begin position="273"/>
            <end position="480"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU004070"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50051"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="701" mass="80252" checksum="9F8211CE0F3750E4" modified="2013-06-26" version="1">MFEEEHFRTLYESKIKQFIKEELSNNLLKGNIFEFDIEKFISKFPEACEINEIIIEKPKEVEDVILDIFKEAYIELYDEDKNLEKIQIAFKNPRGCYKLIEDISSSDINKLVHFEGVIIKAGKVCALLKKACYVCPKCGNIIYRTIHNYFELPKVTCKNCNEKMVIDTEESTYINIQELEIQQPIDLMKNPDDPPRSIKVFLENSRGIYSGRVDVVGTVLKKRTKQNLPVFEIFVKSNYVKISESYQKIEVRDILNKEGIIEILDELGRKKRIVDILSNYLISQIKGYDLVKKAIFLQQIKGAFKFLPDGTPLRRDSHILLITDPGIGKTTMLRRIARLFPQNAYASVTTATGGGLTANVIRESTEIGDGWVVKPGVLVRANEGTACIDELTVDKNVMKYILEAMESQTIHVNKGGINVKLPARCAVLAACNPKHGRFDRNKTVIEQIDIPAPLLSRFDFIFPLMDIPDKKKDDEIAEHILNIHTETATKSYDILGSVEVDGIVVDETLLKYYIIYARSCAYIEKNRDIYLGDFDEMKLIMPYLTEKAKNLIKKYYLEMRKLGEGDNPIPITARQLEAIIRIAEMHAKARLSEKVEEEDAKVAIDIVDYCLKEIAYDPETGKIDLDKIMGTPKSKRDKIEKVLDIIRELSSLNNGIVSEAEIYEKAEEFGLKEKDVEMAIEYLRKTGEIYSPRHGYYAIIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRI3</accession>
    <name>N6VRI3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Pyruvate synthase subunit PorD</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Pyruvate oxidoreductase delta chain</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="11">Pyruvic-ferredoxin oxidoreductase subunit delta</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">J422_01710</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="2">Heterotetramer of one alpha, one beta, one delta and one gamma chain.</text>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96495.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590100.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRI3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00193"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.336"/>
    <dbReference type="OrthoDB" id="23478at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016625">
        <property type="term" value="F:oxidoreductase activity, acting on the aldehyde or oxo group of donors, iron-sulfur protein as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011898">
        <property type="entry name" value="PorD_KorD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053389">
        <property type="entry name" value="Pyruvate_synthase_PorD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040684">
        <property type="entry name" value="PorD_Arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02179">
        <property type="entry name" value="PorD_KorD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43724:SF2">
        <property type="entry name" value="PYRUVATE SYNTHASE SUBUNIT PORD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="7" id="KW-0249">Electron transport</keyword>
    <keyword evidence="8" id="KW-0408">Iron</keyword>
    <keyword evidence="9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="14" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0677">Repeat</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="13">
        <location>
            <begin position="25"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="13">
        <location>
            <begin position="55"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011595"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00044788"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044816"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00044818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN96495.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="86" mass="9891" checksum="3D4527FB41E8598B" modified="2013-06-26" version="1">MVNIAAIIYEPGNSVRNKTGGWRTFRPILDIKKCIKCEKCYIFCPEGAIQEDIDGNFIIDYDYCKGCLICEHECPVKAITRVREEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRI6</accession>
    <name>N6VRI6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Coenzyme F420:L-glutamate ligase-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06035</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000048">
        <property type="protein sequence ID" value="ENN95771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593148.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000048.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRI6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00927"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1175"/>
    <dbReference type="OrthoDB" id="11383at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052618">
        <property type="term" value="F:coenzyme F420-0:L-glutamate ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.100">
        <property type="entry name" value="CofE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002847">
        <property type="entry name" value="F420-0_gamma-glut_ligase-dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012030">
        <property type="entry name" value="UCP006563"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47917">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47917:SF1">
        <property type="entry name" value="COENZYME F420:L-GLUTAMATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01996">
        <property type="entry name" value="F420_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006563">
        <property type="entry name" value="UCP006563"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF144010">
        <property type="entry name" value="CofE-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="181"/>
            <end position="200"/>
        </location>
    </feature>
    <feature type="domain" description="Coenzyme F420:L-glutamate ligase-like" evidence="2">
        <location>
            <begin position="6"/>
            <end position="191"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="285" mass="33045" checksum="43EFCBAE523A7D5A" modified="2013-06-26" version="1">MRAYPIRTRYIKRGENFIPIVLEAIRKSNIRLEDGDVIILSEKMVSTAEGNFIDEEQFKPSILAYLCYYWSKYIWGYLLGKIFLKEEKIKSLRKMPKRESLKHKQMIIETLGLRYALKPYAEGGVDLTNVPLTYANPLPKEPKKWAEMLYHEIKREFNVNVLVMIADTDATYKVLNFYVTALPYAIDGIISGLGFFGFLLGKIAEKLKIGGFIGGTPLAIAGEMRYGIKELARMAYIADRVQNNIKNVNELLERYNTYVITEDILEKMEHTPIVIIKVREEWKRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRK9</accession>
    <name>N6VRK9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Integrase catalytic domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05923</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000046">
        <property type="protein sequence ID" value="ENN95796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592967.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRK9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00987"/>
    <dbReference type="OrthoDB" id="210900at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015074">
        <property type="term" value="P:DNA integration"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001584">
        <property type="entry name" value="Integrase_cat-core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050951">
        <property type="entry name" value="Retrovirus_Pol_polyprotein"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984">
        <property type="entry name" value="PROTEIN CBG26694"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37984:SF5">
        <property type="entry name" value="PROTEIN NYNRIN-LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00665">
        <property type="entry name" value="rve"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50994">
        <property type="entry name" value="INTEGRASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Integrase catalytic" evidence="1">
        <location>
            <begin position="17"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="131" mass="15140" checksum="D16C1AB46757534E" modified="2013-06-26" version="1" fragment="single">RFSLLACYASEKPKRFSTSKPNEMWQIDFKIVDVDGVRYNLLLIIDDHSRFILHAGIYKEATTDVVISALEEYFEKHGTSKKILTDNGTQFIPAKSGISRFQKFLMERKILHIKTSIRHPQTIGKVERISP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRL6</accession>
    <name>N6VRL6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">PUA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_05663</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the methyltransferase superfamily. RlmI family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95801.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRL6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01270"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd11572">
        <property type="entry name" value="RlmI_M_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.750.80">
        <property type="entry name" value="RNA methyltransferase domain (HRMD) like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041532">
        <property type="entry name" value="RlmI-like_PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42873">
        <property type="entry name" value="RIBOSOMAL RNA LARGE SUBUNIT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42873:SF1">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17785">
        <property type="entry name" value="PUA_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="6" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PUA" evidence="9">
        <location>
            <begin position="2"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="10">
        <location>
            <position position="218"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00038091"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95801.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="218" mass="25697" checksum="DF1FC332806F3903" modified="2013-06-26" version="1" fragment="single">MIKLYVDFGGYSAIEKGNLIINRNNIINKEDFDSAELGEVVDIYSKRGKFLGRGFKNPNEVRIMTLKREELNENYIREKIIKANNYRVYELGYKNSYRMVYTQSDWLNGLVIDRFNDIATLQIFNFGIEKYRDVIAETLSELGIEHIYEKSTGRNRRRAGLKDIEGFLKGKKEETVITEGEAKFKVYVKQQKTGFFLDQRENRLLLERFIKEGYRVLD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRR9</accession>
    <name>N6VRR9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Xylose isomerase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05524</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000042">
        <property type="protein sequence ID" value="ENN95856.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592602.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRR9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00745"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1078"/>
    <dbReference type="OrthoDB" id="372143at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001719">
        <property type="entry name" value="AP_endonuc_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050312">
        <property type="entry name" value="IolE/XylAMocC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013022">
        <property type="entry name" value="Xyl_isomerase-like_TIM-brl"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110">
        <property type="entry name" value="HYDROXYPYRUVATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12110:SF21">
        <property type="entry name" value="XYLOSE ISOMERASE-LIKE TIM BARREL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01261">
        <property type="entry name" value="AP_endonuc_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00518">
        <property type="entry name" value="AP2Ec"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Xylose isomerase-like TIM barrel" evidence="1">
        <location>
            <begin position="46"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01261"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95856.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="247" mass="28873" checksum="04C31415520A2967" modified="2013-06-26" version="1">MIGVSMSVFINSDYTLERALSYLENKVKWVELCCDGNVNVMGREEITENFNLKYTLHCPIADFNLSSYREKIRKSSLEFIKDVLETATKVNSEALIIHPGFYIFKHDYDKCLKSLILSLKDLNKLQSEYGINIFIENMPYDMFMFKNPEKEIIENLGELEICFDIGHAFINNNIDKFLAYKDLIGHVHIHDNNGHADEHLPIGFGKIDFEKYKKDLKNINGIKIIELHYKNFENLENCLNKLKNYLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRT9</accession>
    <name>N6VRT9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">Acetylornithine aminotransferase</fullName>
            <shortName evidence="5">ACOAT</shortName>
            <ecNumber evidence="5">2.6.1.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">argD</name>
        <name evidence="7" type="ORF">J422_01570</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate</text>
            <dbReference type="Rhea" id="RHEA:18049"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:29123"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
            <dbReference type="EC" id="2.6.1.11"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
        <text evidence="5">Binds 1 pyridoxal phosphate per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="5">May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.11" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589973.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRT9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00441"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.309"/>
    <dbReference type="OrthoDB" id="85346at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00109"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003992">
        <property type="term" value="F:N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006526">
        <property type="term" value="P:L-arginine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00610">
        <property type="entry name" value="OAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000004">
        <property type="entry name" value="Acetylornithine aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01107">
        <property type="entry name" value="ArgD_aminotrans_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004636">
        <property type="entry name" value="AcOrn/SuccOrn_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005814">
        <property type="entry name" value="Aminotrans_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049704">
        <property type="entry name" value="Aminotrans_3_PPA_site"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050103">
        <property type="entry name" value="Class-III_PLP-dep_AT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00707">
        <property type="entry name" value="argD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002325">
        <property type="entry name" value="PRK01278.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986:SF79">
        <property type="entry name" value="ACETYLORNITHINE AMINOTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11986">
        <property type="entry name" value="AMINOTRANSFERASE CLASS III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00202">
        <property type="entry name" value="Aminotran_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000521">
        <property type="entry name" value="Transaminase_4ab_Lys_Orn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00600">
        <property type="entry name" value="AA_TRANSFER_CLASS_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="1 5 7" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="5" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 5 6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 7" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="100"/>
            <end position="101"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="135"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="217"/>
            <end position="220"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>N(2)-acetyl-L-ornithine</name>
            <dbReference type="ChEBI" id="CHEBI:57805"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="5">
        <location>
            <position position="246"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01107"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003560"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="390" mass="43831" checksum="A56F73D543659D7B" modified="2013-06-26" version="1">MIELERKYHLQVYRRYPVVIVKGRGMEVYDINNKKYLDFIAGIGVNNAGHCHPKIVEAIKNQAENLIHVSNLFYNIPQIELAKRLVGLSKLDKAFFCNSGTEAIEGVIKFARKYANKILNKKGEIITFYNAFHGRTYGALSATPKDRIREGFEPLLEGFKHLPFNNIEALKEGVDNNTIAIMLELVQGEGGVNVAEMEFVKTIADICEDKNILLIVDEIQTGIGRTGKMFAFEHYGIEPDMVTLAKALAGGLPIGAVLLKEEIEKALNYGDHGSTFGGNPLVCSAALANLSVIEELIRDNKVLKKGEYFLNRLKELDYEFIKDVRGLGLMIGVELEFNGNKIVEEMLKRGFLINCTSEKVLRFLPPLIVEKEEIDLLVDNLNEVFKNYSE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRV4</accession>
    <name>N6VRV4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Selenophosphate synthetase</fullName>
            <ecNumber evidence="8">2.7.9.3</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_01470</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.3" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000010">
        <property type="protein sequence ID" value="ENN96590.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRV4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01028"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.289"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004756">
        <property type="term" value="F:selenide, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016260">
        <property type="term" value="P:selenocysteine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02195">
        <property type="entry name" value="SelD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.650.10">
        <property type="entry name" value="PurM-like C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.10">
        <property type="entry name" value="PurM-like, N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010918">
        <property type="entry name" value="PurM-like_C_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036676">
        <property type="entry name" value="PurM-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016188">
        <property type="entry name" value="PurM-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036921">
        <property type="entry name" value="PurM-like_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004536">
        <property type="entry name" value="SPS/SelD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010705">
        <property type="entry name" value="PRK14105.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00476">
        <property type="entry name" value="selD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256:SF0">
        <property type="entry name" value="INACTIVE SELENIDE, WATER DIKINASE-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10256">
        <property type="entry name" value="SELENIDE, WATER DIKINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00586">
        <property type="entry name" value="AIRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02769">
        <property type="entry name" value="AIRS_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036407">
        <property type="entry name" value="Selenphspht_syn"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56042">
        <property type="entry name" value="PurM C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55326">
        <property type="entry name" value="PurM N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0418">Kinase</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0711">Selenium</keyword>
    <keyword evidence="1 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PurM-like N-terminal" evidence="6">
        <location>
            <begin position="6"/>
            <end position="112"/>
        </location>
    </feature>
    <feature type="domain" description="PurM-like C-terminal" evidence="7">
        <location>
            <begin position="125"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF00586"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF02769"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96590.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="301" mass="32714" checksum="B550619C7DFB8974" modified="2013-06-26" version="1">MVVGLGDDAAVIKRGDILIAKTVDVFTPIVDDPYIQGKIAACNSTSDIYAMGFLDIIGVLCIIGFPEKFPIEISKEMLRGFQDFCRENKTSIVGGHTIINPWPLIGGAVTGVGKEEELLTKAGAKEGDLLILTKPLGTQTAMALSRVPDEFKEFIGLSEEEIKEIIDKAIELMTTSNRYALMALRKAEERVGDRIANALTDVTGFGILGHSNEIAKFSKKLIEIKALPCIKKTPELSKMFGHPLLEGYGAETAGGLLISAKKEYKDDLIDELEKVGYAFEVGEVKRGEGKAVLKNPKIIEV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRY2</accession>
    <name>N6VRY2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF531 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05214</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000034">
        <property type="protein sequence ID" value="ENN95921.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592263.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRY2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00306"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1018"/>
    <dbReference type="OrthoDB" id="53060at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007501">
        <property type="entry name" value="DUF531"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04407">
        <property type="entry name" value="DUF531"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006006">
        <property type="entry name" value="UCP006006"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95921.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="166" mass="19068" checksum="3F5BE77C1F0836D0" modified="2013-06-26" version="1">MKRLTIILYNSYDKTRWHEAHKRAIARAAPICYAFDCNLAIMEFPCSMEDIRNLKTTIGNSGEYLEKLIDKNRFFIVDKFLPQFGIPVASTSKPEEKKLVTAKDIAYILRKKPVGIYIGLGRHGLPKKIFDEVKYHLDITEKRISLETCTAIGCIPAVIHTYMMIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS65</accession>
    <name>N6VS65_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6 17">O-phosphoseryl-tRNA(Sec) selenium transferase</fullName>
            <ecNumber evidence="5 17">2.9.1.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 17">Selenocysteine synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14 17">Selenocysteinyl-tRNA(Sec) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="15 17">Sep-tRNA:Sec-tRNA synthase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">J422_00731</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 17">Converts O-phosphoseryl-tRNA(Sec) to selenocysteinyl-tRNA(Sec) required for selenoprotein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="16 17">
            <text>O-phospho-L-seryl-tRNA(Sec) + selenophosphate + H2O = L-selenocysteinyl-tRNA(Sec) + 2 phosphate</text>
            <dbReference type="Rhea" id="RHEA:25041"/>
            <dbReference type="Rhea" id="RHEA-COMP:9743"/>
            <dbReference type="Rhea" id="RHEA-COMP:9947"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:16144"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:78551"/>
            <dbReference type="ChEBI" id="CHEBI:78573"/>
            <dbReference type="EC" id="2.9.1.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 17 19">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3 17">Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec) biosynthesis; selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec) (archaeal/eukaryal route): step 2/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 17">Belongs to the SepSecS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.9.1.2" evidence="5 17"/>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96720.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589820.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS65"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00890"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.141"/>
    <dbReference type="OrthoDB" id="64344at2157"/>
    <dbReference type="UniPathway" id="UPA00906">
        <property type="reaction ID" value="UER00898"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098621">
        <property type="term" value="F:O-phosphoseryl-tRNA(Sec) selenium transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001717">
        <property type="term" value="P:conversion of seryl-tRNAsec to selenocys-tRNAsec"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001514">
        <property type="term" value="P:selenocysteine incorporation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019872">
        <property type="entry name" value="Sec-tRNA_Se_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008829">
        <property type="entry name" value="SepSecS/SepCysS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03531">
        <property type="entry name" value="selenium_SpcS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12944:SF2">
        <property type="entry name" value="O-PHOSPHOSERYL-TRNA(SEC) SELENIUM TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12944">
        <property type="entry name" value="SOLUBLE LIVER ANTIGEN/LIVER PANCREAS ANTIGEN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05889">
        <property type="entry name" value="SepSecS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF017689">
        <property type="entry name" value="SepSecS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 17" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10 17 19" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 17 19" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="12 17" id="KW-0711">Selenium</keyword>
    <keyword evidence="8 17 20" id="KW-0808">Transferase</keyword>
    <keyword evidence="7 17 19" id="KW-0820">tRNA-binding</keyword>
    <feature type="binding site" evidence="18">
        <location>
            <position position="72"/>
        </location>
        <ligand>
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="102"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="266"/>
        </location>
        <ligand>
            <name>tRNA</name>
            <dbReference type="ChEBI" id="CHEBI:17843"/>
        </ligand>
        <ligandPart>
            <name>tRNA variable arm</name>
        </ligandPart>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="307"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="May act as a substrate filter by repelling compounds with a negatively charged alpha-carboxylate" evidence="19">
        <location>
            <position position="71"/>
        </location>
    </feature>
    <feature type="modified residue" description="N6-(pyridoxal phosphate)lysine" evidence="19">
        <location>
            <position position="278"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004822"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007037"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00012464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00021963"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023266"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00032048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00032693"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00048808"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRNR" id="PIRNR017689"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR017689-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="PIRSR" id="PIRSR017689-50"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ENN96720.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="435" mass="48913" checksum="193113E2C7FF1537" modified="2013-06-26" version="1">MLNLNFTGLIPENMEKRGKLTLKDNLNIIEEIFNQRKIPKEGLDDDKIKLLLKILSLMDTDKDPKVIQIGEREARIASRIQNELVAGFCHGIGRSGNLIDAQPKAPGASIMYALTNKILENFLKNLGLKVNAIATPVATGMSLALCLSAARKRYYSNVVIYPYASHKSPIKATSFIGMRMRLVETKLYEDAVRVDVSDIENAIKKEIKENNNPCVLSTLTFFPPRESDDVIEISKICEEFNIPHIINGAYAIQNKFYIDKLNKAFKYRVDAVVSSSDKNLFTPIGGGIIYSRDKEFLRETSLCYPGRACATPVVNILISFLSLGMNKYLDLMKKQKECKKLLDNLLNELAEKKGEKVLNVKNPISSAITTKKDPVEVAAKLYNLRITGPRGVRKNDKFGTCYLGEYPYDYIVVNSAIGVKEEDIYKVVEKLERVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS83</accession>
    <name>N6VS83_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">HesB-like (Seleno)protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04700</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96011.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS83"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00959"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.919"/>
    <dbReference type="OrthoDB" id="61368at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96011.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="53" mass="6033" checksum="87CD701A4F27275A" modified="2013-06-26" version="1">MVIKDPSPNDELVYDKEFKIYVDPIVKQFLDGGKIKLVYKKSIFGEGVNVVIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSC8</accession>
    <name>N6VSC8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoesterase</fullName>
            <ecNumber evidence="1">3.1.4.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_04273</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the metallophosphoesterase superfamily. YfcE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.4.-" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96056.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591740.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSC8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00668"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.836"/>
    <dbReference type="OrthoDB" id="19174at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00841">
        <property type="entry name" value="MPP_YfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024654">
        <property type="entry name" value="Calcineurin-like_PHP_lpxH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041802">
        <property type="entry name" value="MPP_YfcE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000979">
        <property type="entry name" value="Phosphodiesterase_MJ0936/Vps29"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00040">
        <property type="entry name" value="yfcE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11124">
        <property type="entry name" value="VACUOLAR SORTING PROTEIN VPS29"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12850">
        <property type="entry name" value="Metallophos_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="147"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="RuleBase" id="RU362039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12850"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96056.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="159" mass="18005" checksum="E76FAB33A66A2885" modified="2013-06-26" version="1">MIIGVISDTHIYDRALELPKTVFDEFSNVDLIIHCGDITDPEVLKCLNDIAKVIAVKGNMDYLDLPRKEILTIDKYKIGVIHGDIIYPRGDKLKLRLLAKEMGVNILISGHTHWPFIDHYNDTLLLNPGSPTVPRCSFKSIMKLSLEDKVEAKIIPVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSF1</accession>
    <name>N6VSF1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">2,3-bisphosphoglycerate-independent phosphoglycerate mutase</fullName>
            <shortName evidence="7">BPG-independent PGAM</shortName>
            <shortName evidence="7">Phosphoglyceromutase</shortName>
            <shortName evidence="7">aPGAM</shortName>
            <ecNumber evidence="7">5.4.2.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">apgM</name>
        <name evidence="9" type="ORF">J422_04348</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2 7">Catalyzes the interconversion of 2-phosphoglycerate and 3-phosphoglycerate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>(2R)-2-phosphoglycerate = (2R)-3-phosphoglycerate</text>
            <dbReference type="Rhea" id="RHEA:15901"/>
            <dbReference type="ChEBI" id="CHEBI:58272"/>
            <dbReference type="ChEBI" id="CHEBI:58289"/>
            <dbReference type="EC" id="5.4.2.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 7">Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 3/5.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4 7">Belongs to the BPG-independent phosphoglycerate mutase family. A-PGAM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.12" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96071.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591772.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSF1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00653"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.852"/>
    <dbReference type="OrthoDB" id="52918at2157"/>
    <dbReference type="UniPathway" id="UPA00109">
        <property type="reaction ID" value="UER00186"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004619">
        <property type="term" value="F:phosphoglycerate mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006096">
        <property type="term" value="P:glycolytic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd16011">
        <property type="entry name" value="iPGM_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.720.10">
        <property type="entry name" value="Alkaline Phosphatase, subunit A"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2130">
        <property type="entry name" value="Metalloenzyme domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01402_A">
        <property type="entry name" value="ApgM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017850">
        <property type="entry name" value="Alkaline_phosphatase_core_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023665">
        <property type="entry name" value="ApgAM_prokaryotes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006124">
        <property type="entry name" value="Metalloenzyme"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004456">
        <property type="entry name" value="Pglycerate_mutase_ApgM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042253">
        <property type="entry name" value="Pglycerate_mutase_ApgM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00306">
        <property type="entry name" value="apgM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003104">
        <property type="entry name" value="PRK04024.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209">
        <property type="entry name" value="COFACTOR-INDEPENDENT PHOSPHOGLYCERATE MUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31209:SF0">
        <property type="entry name" value="METALLOENZYME DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01676">
        <property type="entry name" value="Metalloenzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10143">
        <property type="entry name" value="PhosphMutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006392">
        <property type="entry name" value="IPGAM_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53649">
        <property type="entry name" value="Alkaline phosphatase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7" id="KW-0324">Glycolysis</keyword>
    <keyword evidence="6 7 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metalloenzyme" evidence="8">
        <location>
            <begin position="1"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004798"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005524"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023152"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96071.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="405" mass="45061" checksum="1F98E251AEB8C2F3" modified="2013-06-26" version="1">MKIIIFIIDGLGDRPNEKGETPLKIAKKPTMNRIAEEGICGLMNAIDIGIRPGSDTAHLAILGYDPYKVYTGRGPLEAFGLGLDLKEGDIAFRCNFATVDDNFIVKDRRAGRLDFETAKILEKEIDGLEIDGVKVIFRSKGYRGALVLRGEGLDYMVTDADPHKDNVKVEEVKPLRKEAEKTAEILNKLLKIIYNKLKDHPVNIEREKKGLPPANIILPRGAGIVPKVEKFYEKYKMKGAVICGTALIKGIGKMLGLDVIEVEGANGTPNSNIMGKAKALLNNLDKYDFFLINVKAADEAGHDGKFELKKEIIEKIDNMLSYIFENINKDSVYFVLTGDHSTPIEVKDHSADPVPIVIWGKSVRKDDVKEFDEFACAKGGLHWIKGEHVMRILLDLTNRNEKFGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="63" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSG0</accession>
    <name>N6VSG0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">S-inosyl-L-homocysteine hydrolase</fullName>
            <shortName evidence="5">SIHH</shortName>
            <ecNumber evidence="5">3.13.1.9</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_04433</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the hydrolysis of S-inosyl-L-homocysteine (SIH) to L-homocysteine (Hcy) and inosine. Likely functions in a S-adenosyl-L-methionine (SAM) recycling pathway from S-adenosyl-L-homocysteine (SAH) produced from SAM-dependent methylation reactions. Can also catalyze the reverse reaction in vitro, i.e. the synthesis of SIH from Hcy and inosine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>S-inosyl-L-homocysteine + H2O = L-homocysteine + inosine</text>
            <dbReference type="Rhea" id="RHEA:59828"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:17596"/>
            <dbReference type="ChEBI" id="CHEBI:57985"/>
            <dbReference type="ChEBI" id="CHEBI:58199"/>
            <dbReference type="EC" id="3.13.1.9"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="5 6">
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </cofactor>
        <text evidence="5 6">Binds 1 NAD(+) per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="5">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="miscellaneous">
        <text evidence="5">SAH is a product of SAM methyltransferases and is known to be a feedback inhibitor of these enzymes. As a result of this inhibition, organisms have evolved efficient enzymes to metabolize SAH via different pathways. The pathway found in methanogens differs from the canonical pathway, it uses the deamination of S-adenosyl-L-homocysteine to form S-inosyl-L-homocysteine for the regeneration of SAM from S-adenosyl-L-homocysteine.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 5 7">Belongs to the adenosylhomocysteinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.13.1.9" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96086.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591804.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSG0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00637"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.869"/>
    <dbReference type="OrthoDB" id="8479at2157"/>
    <dbReference type="UniPathway" id="UPA00315"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004013">
        <property type="term" value="F:adenosylhomocysteinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016802">
        <property type="term" value="F:trialkylsulfonium hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006556">
        <property type="term" value="P:S-adenosylmethionine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033353">
        <property type="term" value="P:S-adenosylmethionine cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00401">
        <property type="entry name" value="SAHH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.720:FF:000004">
        <property type="entry name" value="Adenosylhomocysteinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1480">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00563">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042172">
        <property type="entry name" value="Adenosylhomocyst_ase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000043">
        <property type="entry name" value="Adenosylhomocysteinase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015878">
        <property type="entry name" value="Ado_hCys_hydrolase_NAD-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020082">
        <property type="entry name" value="S-Ado-L-homoCys_hydrolase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00936">
        <property type="entry name" value="ahcY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004005">
        <property type="entry name" value="PRK05476.2-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23420:SF0">
        <property type="entry name" value="ADENOSYLHOMOCYSTEINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05221">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00670">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001109">
        <property type="entry name" value="Ad_hcy_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00996">
        <property type="entry name" value="AdoHcyase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00997">
        <property type="entry name" value="AdoHcyase_NAD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52283">
        <property type="entry name" value="Formate/glycerate dehydrogenase catalytic domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00738">
        <property type="entry name" value="ADOHCYASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00739">
        <property type="entry name" value="ADOHCYASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 5 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 5 6" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="S-adenosyl-L-homocysteine hydrolase NAD binding" evidence="8">
        <location>
            <begin position="183"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="148"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <begin position="149"/>
            <end position="151"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="182"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <position position="183"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5">
        <location>
            <begin position="212"/>
            <end position="217"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="214"/>
            <end position="219"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="235"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <begin position="291"/>
            <end position="293"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="5 6">
        <location>
            <position position="337"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="344"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR001109-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU004166"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM00997"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96086.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="415" mass="47001" checksum="8AC7D2789E38DFFE" modified="2013-06-26" version="1">MYNVRDISLWKEGEKKIEWAKRHMPVLNLIREKFKEEKPFKGITIGMALHLEAKTAVLAETLMEGGAEIAITGCNPLSTQDDVAAACAKKGMHVYAWRGETREEYYENLNKVLDHEPDIIIDDGCDLIFLVHTKRTELLEKIKGACEETTTGIIRLKAMEKERALKFPVMDVNDAYTKHLFDNRYGTGQSALDGIIRTTNLLIAGKTVVVAGYGWCGRGIAMRAKGLGADVIVTEVDPIRALEARMDGFRVMKMIEAAKYGDIFITATGCKDVIREEHFLKMKDGAILCNAGHFDNEVSKKDLEKLAKSIREVRKNILEYDLGDKRLYLLGEGRLVNLACADGHPIEVMDMSFSNQALAARYLLENYKKLEPKVYRIPYEQDLFIAKLKLKSLGIEIDELTEEQKKYLNDWREGT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSQ7</accession>
    <name>N6VSQ7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Polysaccharide pyruvyl transferase CsaB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_03478</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000022">
        <property type="protein sequence ID" value="ENN96226.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591144.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSQ7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01338"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.678"/>
    <dbReference type="OrthoDB" id="330594at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007345">
        <property type="entry name" value="Polysacch_pyruvyl_Trfase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36836">
        <property type="entry name" value="COLANIC ACID BIOSYNTHESIS PROTEIN WCAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36836:SF1">
        <property type="entry name" value="COLANIC ACID BIOSYNTHESIS PROTEIN WCAK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04230">
        <property type="entry name" value="PS_pyruv_trans"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="82"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="111"/>
            <end position="127"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="154"/>
        </location>
    </feature>
    <feature type="domain" description="Polysaccharide pyruvyl transferase" evidence="2">
        <location>
            <begin position="35"/>
            <end position="332"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF04230"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96226.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="374" mass="42638" checksum="BDB576C60F6D2078" modified="2013-06-26" version="1">MYKFYSDVDKALSLVPKDFIYGKKILIIGNYGDGNLGDEAMLKVLYNYLISNGVNLIYVITKNPAFYQRYIKNSRFQPVLATNLLAVLKAFLVCDTIIIGGGSIYSRYSGLGVYIASLLSIFIRLLLNKKILILGIGFSSSTPLLLKFICKYLFKICNFIYVRDSISFKNIRNLGIKKKIIVSKDLVFSSFIQPFDKKLGKKILEKEGIKIDKGKRLVGLALNFSGKKEKDEKIIAIFKEIIPWLIDKYNAEILFFTFCPAFVSKISDTFLFKKIYGELGNKYKKKCHILSYYLPEEVMSIFMNLDLVIGSRFHSMVFAYKVGTPFVGISYEEKCSAFLSEIRKPLINIESLDAKTLKGEISRILGGLKYERTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT83</accession>
    <name>N6VT83_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Metallophosphoesterase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02484</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96406.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590444.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT83"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00776"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.486"/>
    <dbReference type="OrthoDB" id="50367at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.21.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004843">
        <property type="entry name" value="Calcineurin-like_PHP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029052">
        <property type="entry name" value="Metallo-depent_PP-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523:SF1">
        <property type="entry name" value="CALCINEURIN-LIKE PHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37523">
        <property type="entry name" value="METALLOPHOSPHOESTERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00149">
        <property type="entry name" value="Metallophos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56300">
        <property type="entry name" value="Metallo-dependent phosphatases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Calcineurin-like phosphoesterase" evidence="1">
        <location>
            <begin position="1"/>
            <end position="171"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00149"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96406.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="207" mass="23448" checksum="1AA91B2005B27FFA" modified="2013-06-26" version="1">MRIVGITDLHGRFPKSIKEISADVLVVSGDITHFGKGIEVIEDLAELSDNMEVLCVPGNCDTEEVIDELNSYNLNIDRKVKKIGEINFVGFGGSNRTPFNTVYEWDEEECYNILKRIMKGLKNICLITHAPPYNTMADYILDKDIHVGSKSIRRVIEEEDVILNACGHIHEARCIDKIGKTIIVNPSPKSYFIYDTKKEIVVLEDYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT87</accession>
    <name>N6VT87_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Asparaginyl/glutamyl-tRNA amidotransferase subunit C</fullName>
            <ecNumber evidence="1">6.3.5.7</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="primary">gatC</name>
        <name evidence="1" type="ORF">J422_02509</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96411.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590455.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT87"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00770"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.491"/>
    <dbReference type="OrthoDB" id="64808at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006450">
        <property type="term" value="P:regulation of translational fidelity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036113">
        <property type="entry name" value="Asp/Glu-ADT_sf_sub_c"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003837">
        <property type="entry name" value="GatC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010120">
        <property type="entry name" value="Glu-ADT_subunit_C_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01827">
        <property type="entry name" value="gatC_rel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000681">
        <property type="entry name" value="PRK00034.3-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02686">
        <property type="entry name" value="GatC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141000">
        <property type="entry name" value="Glu-tRNAGln amidotransferase C subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0436">Ligase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96411.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="71" mass="8452" checksum="52BC34C56298BDCC" modified="2013-06-26" version="1">MREEAEKIIKMFSEILEKYDLEIEESYYIIDEKNVLRDDKAEPSNMREKILNIAPKVKDGYIVVEKGSWVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTB6</accession>
    <name>N6VTB6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Transcriptional regulator, ArsR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_02385</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96436.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590349.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTB6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00358"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.469"/>
    <dbReference type="OrthoDB" id="46231at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051081">
        <property type="entry name" value="HTH_MetalResp_TranReg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033788">
        <property type="entry name" value="HTH_metalloreg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154">
        <property type="entry name" value="TRANSCRIPTIONAL REGULATOR, ARSR FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33154:SF33">
        <property type="entry name" value="TRANSCRIPTIONAL REPRESSOR SDPR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01022">
        <property type="entry name" value="HTH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00778">
        <property type="entry name" value="HTHARSR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00418">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0804">Transcription</keyword>
    <keyword evidence="1" id="KW-0805">Transcription regulation</keyword>
    <feature type="domain" description="HTH arsR-type" evidence="4">
        <location>
            <begin position="1"/>
            <end position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96436.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="89" mass="10321" checksum="A19A0FDFB2A253CE" modified="2013-06-26" version="1">MEKYERAAEVFKAFGDPTRLMILKLLAENGSMCVCKIIEELKKPQPTISHHLNILKKAGIVRARKEGTWNFYYITNDKVKEIIKLVDEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTG5</accession>
    <name>N6VTG5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ribonuclease P protein component 4</fullName>
            <shortName evidence="8">RNase P component 4</shortName>
            <ecNumber evidence="8">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Rpp21</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">rnp4</name>
        <name evidence="9" type="ORF">J422_02265</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="8">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the eukaryotic/archaeal RNase P protein component 4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96476.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590285.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTG5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01201"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.445"/>
    <dbReference type="OrthoDB" id="10058at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.420">
        <property type="entry name" value="Immunoglobulin FC, subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00757">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016432">
        <property type="entry name" value="RNP4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007175">
        <property type="entry name" value="Rpr2/Snm1/Rpp21"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742:SF0">
        <property type="entry name" value="RIBONUCLEASE P PROTEIN SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14742">
        <property type="entry name" value="RIBONUCLEASE P SUBUNIT P21"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04032">
        <property type="entry name" value="Rpr2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004878">
        <property type="entry name" value="RNase_P_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="6 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 8" id="KW-0540">Nuclease</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="7 8" id="KW-0862">Zinc</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="90"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00757"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96476.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="115" mass="14296" checksum="5A536A2F17E25F1A" modified="2013-06-26" version="1">MGNRKRLIKIAYERIDRLMSLAEEEAKKGNWERVKRYVYLARRIAMKMRIKFPKKWKRRFCKKCLTFWIFGKNARVRVKSKRYPHIVITCLECGKIYRIPMIKEKKEKRRSKWEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTP4</accession>
    <name>N6VTP4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3 8">F420-dependent methylenetetrahydromethanopterin dehydrogenase</fullName>
            <shortName evidence="8">MTD</shortName>
            <ecNumber evidence="2 8">1.5.98.1</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7 8">Coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin dehydrogenase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">mtd</name>
        <name evidence="10" type="ORF">J422_01990</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to methylene-H(4)MPT.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8">
            <text>5,10-methylenetetrahydromethanopterin + oxidized coenzyme F420-(gamma-L-Glu)(n) + 2 H(+) = 5,10-methenyl-5,6,7,8-tetrahydromethanopterin + reduced coenzyme F420-(gamma-L-Glu)(n)</text>
            <dbReference type="Rhea" id="RHEA:16721"/>
            <dbReference type="Rhea" id="RHEA-COMP:12939"/>
            <dbReference type="Rhea" id="RHEA-COMP:14378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57818"/>
            <dbReference type="ChEBI" id="CHEBI:58337"/>
            <dbReference type="ChEBI" id="CHEBI:133980"/>
            <dbReference type="ChEBI" id="CHEBI:139511"/>
            <dbReference type="EC" id="1.5.98.1"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">One-carbon metabolism; methanogenesis from CO(2); 5,10-methylene-5,6,7,8-tetrahydromethanopterin from 5,10-methenyl-5,6,7,8-tetrahydromethanopterin (coenzyme F420 route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8">Belongs to the MTD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.5.98.1" evidence="2 8"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96541.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590164.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTP4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00243"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.390"/>
    <dbReference type="OrthoDB" id="49844at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00695"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030268">
        <property type="term" value="F:methylenetetrahydromethanopterin dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006730">
        <property type="term" value="P:one-carbon metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.140.120">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10830">
        <property type="entry name" value="F420-dependent methylenetetrahydromethanopterin dehydrogenase (MTD)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00058">
        <property type="entry name" value="MTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002844">
        <property type="entry name" value="MTD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036080">
        <property type="entry name" value="MTD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002162">
        <property type="entry name" value="PRK00994.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01993">
        <property type="entry name" value="MTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005627">
        <property type="entry name" value="MTD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102324">
        <property type="entry name" value="F420-dependent methylenetetrahydromethanopterin dehydrogenase (MTD)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="4 8" id="KW-0554">One-carbon metabolism</keyword>
    <keyword evidence="6 8 10" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="region of interest" description="Disordered" evidence="9">
        <location>
            <begin position="250"/>
            <end position="275"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012904"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00014062"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022563"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031410"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00058"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96541.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="275" mass="30199" checksum="31B46ED28C9FF23F" modified="2013-06-26" version="1">MIKIAIIKCGNIGMSPMIDLSLDERADREDIIIRVFGSGPKMDPESVEEVTKKAIEENPNLIIYIGPNPSAPGPKKAIELLKECKIPSIIIGDAPGIKIKDKIAEAGLGYIIVKCDSMIGARREFLDPTEVGFFNSDIIKVLAGTGALRAVQMAIDKVISEIKEGKEPELPKLIIDEEKAVEAMEFTNPYAKAKAMAAFVIAEKVGDIDVKGCFMVKEAEKYIPIVASAHEMLRYAAKLIDEARELEKANDAVSRKPHARDGRVLSKKRLMEKPQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTX9</accession>
    <name>N6VTX9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">5-carboxymethyl-2-hydroxymuconate Delta-isomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_01263</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the FAH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96626.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589916.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTX9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00037"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.247"/>
    <dbReference type="OrthoDB" id="6242at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.850.10:FF:000002">
        <property type="entry name" value="2-hydroxyhepta-2,4-diene-1,7-dioate isomerase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.850.10">
        <property type="entry name" value="Fumarylacetoacetase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051121">
        <property type="entry name" value="FAH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011234">
        <property type="entry name" value="Fumarylacetoacetase-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036663">
        <property type="entry name" value="Fumarylacetoacetase_C_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796:SF4">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42796">
        <property type="entry name" value="FUMARYLACETOACETATE HYDROLASE DOMAIN-CONTAINING PROTEIN 2A-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01557">
        <property type="entry name" value="FAA_hydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56529">
        <property type="entry name" value="FAH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0413">Isomerase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Fumarylacetoacetase-like C-terminal" evidence="3">
        <location>
            <begin position="10"/>
            <end position="203"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF01557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96626.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="204" mass="23447" checksum="AA16F1FFD34DE6A8" modified="2013-06-26" version="1">MDIRKINPTKIVCVGLNYIDHAKELNLPIPDEPIIFLKPPSSIIYDGDYIIKPKICKRLDHEVELAFIIKKKCKDVKKDHEDYILGYTILNDVTARDLQKKDGQWTRAKSFDTFCPIGPRIVKDIDPMNLKIECRVNNEVRQKSNTKNMIFDIYYLLEFVSSIMTLYPGDIISTGTPPGVSEIKRGDVVECEIENIGILKNYVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTZ7</accession>
    <name>N6VTZ7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 10">Translation initiation factor 2 subunit alpha</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8 10">aIF2-alpha</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9 10">eIF-2-alpha</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">eif2a</name>
        <name evidence="12" type="ORF">J422_01116</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 10">eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Heterotrimer composed of an alpha, a beta and a gamma chain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the eIF-2-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589891.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTZ7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00819"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.218"/>
    <dbReference type="OrthoDB" id="84794at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043022">
        <property type="term" value="F:ribosome binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04452">
        <property type="entry name" value="S1_IF2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000015">
        <property type="entry name" value="Eukaryotic translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.150.190:FF:000006">
        <property type="entry name" value="Translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.1130:FF:000002">
        <property type="entry name" value="Translation initiation factor 2 subunit alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1130">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.190">
        <property type="entry name" value="Translation initiation factor 2, subunit 1, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00231">
        <property type="entry name" value="eIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003029">
        <property type="entry name" value="S1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044126">
        <property type="entry name" value="S1_IF2_alpha"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022964">
        <property type="entry name" value="TIF2_asu_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024055">
        <property type="entry name" value="TIF2_asu_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024054">
        <property type="entry name" value="TIF2_asu_middle_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011488">
        <property type="entry name" value="TIF_2_asu"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003062">
        <property type="entry name" value="PRK03987.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003063">
        <property type="entry name" value="PRK03987.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003064">
        <property type="entry name" value="PRK03987.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10602:SF0">
        <property type="entry name" value="EUKARYOTIC TRANSLATION INITIATION FACTOR 2 SUBUNIT 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07541">
        <property type="entry name" value="EIF_2_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00575">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00316">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110993">
        <property type="entry name" value="eIF-2-alpha, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF116742">
        <property type="entry name" value="eIF2alpha middle domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50126">
        <property type="entry name" value="S1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10 12" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="7 10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 10" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="S1 motif" evidence="11">
        <location>
            <begin position="9"/>
            <end position="80"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007223"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011243"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00013678"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00030860"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00033333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="262" mass="30073" checksum="3C48F512C776C4A3" modified="2013-06-26" version="1">MRKEFPEEGDIVIGVVKEVKPYGAFVELLEYPGKEGMIHISEVTSGWVKNIRDHVKPGQRVVAKVLRVDEKKGHIDLSLKRISEQQRREKIQEWKRFQRASKMLERAAEKLGKSLEEAWDEVGYLLEDEFGELYNAFETLVIEGKEALEGLDISEDWKNVLYEVAKESIELTNVEVEGIIEMTCFEPDGIDRIKKALKEALKANPYEDVEVKITYVGAPKYRVEVIAPDYKSGEEVFKNVCESAIKAIKKLKGEGKYYRVKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU08</accession>
    <name>N6VU08_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">PHP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01201</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589906.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU08"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00804"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.235"/>
    <dbReference type="OrthoDB" id="63337at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0035312">
        <property type="term" value="F:5'-3' DNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004534">
        <property type="term" value="F:5'-3' RNA exonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07432">
        <property type="entry name" value="PHP_HisPPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052018">
        <property type="entry name" value="PHP_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003141">
        <property type="entry name" value="Pol/His_phosphatase_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924">
        <property type="entry name" value="EXONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42924:SF3">
        <property type="entry name" value="POLYMERASE_HISTIDINOL PHOSPHATASE N-TERMINAL DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13263">
        <property type="entry name" value="PHP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00481">
        <property type="entry name" value="POLIIIAc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="251"/>
            <end position="277"/>
        </location>
    </feature>
    <feature type="domain" description="Polymerase/histidinol phosphatase N-terminal" evidence="2">
        <location>
            <begin position="3"/>
            <end position="79"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="291" mass="32852" checksum="40B6A247982B6782" modified="2013-06-26" version="1">MKADLHIHTKYSGISKFWKLKFPDSVEEPKNILKVAKKKRIDIVAITDHNTIRGGLETKKIAKDYGVEVIVGSEIMSKDGEIIGLFLNEEIPKGLSAEETIERIKEQGGLAIAPHPYSPICKGLGDKIFSLDLDGVEVFNAYHRDGIINNIALSKVIKNYHKKRYAFIGGSDAHIAMMVGNAYTLFEGNSADDLYKAIKKKETTFSGNLTPLYQAILWSYKVVLKSEKMIISSLFFNGNDYIKSIKLYKKLLAMFGGFLYIFTPLPIFSGFLGNYYLKRLAKKKMKNYLLE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU78</accession>
    <name>N6VU78_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Prephenate dehydrogenase</fullName>
            <ecNumber evidence="3">1.3.1.12</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_00501</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.3.1.12" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96751.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589782.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU78"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00052"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.96"/>
    <dbReference type="OrthoDB" id="24743at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070403">
        <property type="term" value="F:NAD+ binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008977">
        <property type="term" value="F:prephenate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004665">
        <property type="term" value="F:prephenate dehydrogenase (NADP+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006571">
        <property type="term" value="P:tyrosine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3660.10">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.380">
        <property type="entry name" value="Ferrodoxin-fold anticodon-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008927">
        <property type="entry name" value="6-PGluconate_DH-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005121">
        <property type="entry name" value="Fdx_antiC-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036690">
        <property type="entry name" value="Fdx_antiC-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046825">
        <property type="entry name" value="PDH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046826">
        <property type="entry name" value="PDH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008299">
        <property type="entry name" value="Prep_DH/arog_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050812">
        <property type="entry name" value="Preph/Arog_dehydrog"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003099">
        <property type="entry name" value="Prephen_DH"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006408">
        <property type="entry name" value="PRK08655.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006410">
        <property type="entry name" value="PRK08655.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21363:SF0">
        <property type="entry name" value="PREPHENATE DEHYDROGENASE [NADP(+)]"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20463">
        <property type="entry name" value="PDH_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02153">
        <property type="entry name" value="PDH_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006549">
        <property type="entry name" value="PDH_arog_dh_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00896">
        <property type="entry name" value="FDX-ACB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48179">
        <property type="entry name" value="6-phosphogluconate dehydrogenase C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54991">
        <property type="entry name" value="Anticodon-binding domain of PheRS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51176">
        <property type="entry name" value="PDH_ADH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate/arogenate dehydrogenase" evidence="2">
        <location>
            <begin position="1"/>
            <end position="283"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96751.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="440" mass="50491" checksum="5157AD91F8B2AB28" modified="2013-06-26" version="1">MIISIIGGTDGLGKWFARYLKGKGFDVIVTGRDVEKGKKVERELGVKFINDNIEAAKKADIVIIAVPINVTEKVIKEVAPHVRDGCLLMDITSIKEIPTKAMEEYANKNVVIIPTHPMFGPSAPSLFGQVIILTPLERHKNTEWFKKVYNFLKKEGAKIIITTPEKHDRIMAVVQGLTHFSFISLAYTLKELNVDIKESRKYSSPVYELMLSIIARIIGQNPYLYADIQMFNKRIGEIHKTFINCCLELSNVVKNKDREKFVKIMKEAAKHFGSEAKRGVYYSDKAIFALTNELNRLVDMIGEEVAIRNIDSNNVHFGILKGVDGDYLILDKNGKLEKFNVLRVEVFDGEKLKELKKRYLKRIYFDVSVLLKDNVDENTILDLLKSKFDIEIIDVYKGENIEKGWKSVTFRIFGYKKDELREKEKEFIKIIKNIGGKLRY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU99</accession>
    <name>N6VU99_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Coenzyme A biosynthesis bifunctional protein CoaBC</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">DNA/pantothenate metabolism flavoprotein</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">Phosphopantothenoylcysteine synthetase/decarboxylase</fullName>
            <shortName evidence="3">PPCS-PPCDC</shortName>
        </alternativeName>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenoylcysteine decarboxylase</fullName>
                <shortName evidence="3">PPC decarboxylase</shortName>
                <shortName evidence="3">PPC-DC</shortName>
                <ecNumber evidence="3">4.1.1.36</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaC</fullName>
            </alternativeName>
        </domain>
        <domain>
            <recommendedName>
                <fullName evidence="3">Phosphopantothenate--cysteine ligase</fullName>
                <ecNumber evidence="3">6.3.2.5</ecNumber>
            </recommendedName>
            <alternativeName>
                <fullName evidence="3">CoaB</fullName>
            </alternativeName>
            <alternativeName>
                <fullName evidence="3">Phosphopantothenoylcysteine synthetase</fullName>
                <shortName evidence="3">PPC synthetase</shortName>
                <shortName evidence="3">PPC-S</shortName>
            </alternativeName>
        </domain>
    </protein>
    <gene>
        <name evidence="3" type="primary">coaBC</name>
        <name evidence="6" type="ORF">J422_00376</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes two sequential steps in the biosynthesis of coenzyme A. In the first step cysteine is conjugated to 4'-phosphopantothenate to form 4-phosphopantothenoylcysteine. In the second step the latter compound is decarboxylated to form 4'-phosphopantotheine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(R)-4'-phosphopantothenate + L-cysteine + CTP = N-[(R)-4-phosphopantothenoyl]-L-cysteine + CMP + diphosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19397"/>
            <dbReference type="ChEBI" id="CHEBI:10986"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:35235"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="EC" id="6.3.2.5"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>N-[(R)-4-phosphopantothenoyl]-L-cysteine + H(+) = (R)-4'-phosphopantetheine + CO2</text>
            <dbReference type="Rhea" id="RHEA:16793"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:59458"/>
            <dbReference type="ChEBI" id="CHEBI:61723"/>
            <dbReference type="EC" id="4.1.1.36"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
        <text evidence="3">Binds 1 FMN per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Cofactor biosynthesis; coenzyme A biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the C-terminal section; belongs to the PPC synthetase family.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">In the N-terminal section; belongs to the HFCD (homo-oligomeric flavin containing Cys decarboxylase) superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.1.1.36" evidence="3"/>
    <dbReference type="EC" id="6.3.2.5" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96771.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589757.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU99"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00705"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.72"/>
    <dbReference type="OrthoDB" id="10536at2157"/>
    <dbReference type="UniPathway" id="UPA00241"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071513">
        <property type="term" value="C:phosphopantothenoylcysteine decarboxylase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010181">
        <property type="term" value="F:FMN binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004632">
        <property type="term" value="F:phosphopantothenate--cysteine ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004633">
        <property type="term" value="F:phosphopantothenoylcysteine decarboxylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015937">
        <property type="term" value="P:coenzyme A biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015941">
        <property type="term" value="P:pantothenate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10300">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1950">
        <property type="entry name" value="Flavin prenyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02225">
        <property type="entry name" value="CoaBC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035929">
        <property type="entry name" value="CoaB-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005252">
        <property type="entry name" value="CoaBC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007085">
        <property type="entry name" value="DNA/pantothenate-metab_flavo_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036551">
        <property type="entry name" value="Flavin_trans-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003382">
        <property type="entry name" value="Flavoprotein"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00521">
        <property type="entry name" value="coaBC_dfp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359">
        <property type="entry name" value="HOMO-OLIGOMERIC FLAVIN CONTAINING CYS DECARBOXYLASE FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR14359:SF6">
        <property type="entry name" value="PHOSPHOPANTOTHENOYLCYSTEINE DECARBOXYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04127">
        <property type="entry name" value="DFP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02441">
        <property type="entry name" value="Flavoprotein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102645">
        <property type="entry name" value="CoaB-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52507">
        <property type="entry name" value="Homo-oligomeric flavin-containing Cys decarboxylases, HFCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3" id="KW-0210">Decarboxylase</keyword>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="3" id="KW-0288">FMN</keyword>
    <keyword evidence="3 6" id="KW-0436">Ligase</keyword>
    <keyword evidence="2 3" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0511">Multifunctional enzyme</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Flavoprotein" evidence="4">
        <location>
            <begin position="5"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="DNA/pantothenate metabolism flavoprotein C-terminal" evidence="5">
        <location>
            <begin position="172"/>
            <end position="372"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenoylcysteine decarboxylase" evidence="3">
        <location>
            <begin position="1"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="region of interest" description="Phosphopantothenate--cysteine ligase" evidence="3">
        <location>
            <begin position="175"/>
            <end position="382"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="260"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="269"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="302"/>
        </location>
        <ligand>
            <name>CTP</name>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022793"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02225"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF02441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF04127"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96771.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="382" mass="43347" checksum="FAF960056C8C0FD1" modified="2013-06-26" version="1">MLRNKKIVVALTASIAAVEAFRLIRELKKLGAEVYCIATKETFDIIGRKALEFTADKVMDEISGDVEHVSLYNNCDLLIIYPATANIISKISSKIADNIVNTTALVFLNHKPIIIAPAMHINMYENIREHLEKLSNYNHVYILQPRFENGKAKIAKVDDVIKKIFEIFNKDKKRVLILNGATAEFIDDVRVITNLSSGKMGNALAEALLKYFNVEMILGIGEKPRWVKCYRALTSEEMLNKALDLGKDFDIIISSAAISDFITEKLEGKVSSDNELIIKLKPNKKILKELRKRFKDKIIIGFKAEYNVDKDELIRRAKRRMEEYGLDMIIANDLSKYYFGSDETEVYAITKDKIELISGKKREVAEKLAEIINVITNGSCKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VUG5</accession>
    <name>N6VUG5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Cobalt ABC transporter permease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_00275</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96836.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589742.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VUG5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01108"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.55"/>
    <dbReference type="OrthoDB" id="147966at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043190">
        <property type="term" value="C:ATP-binding cassette (ABC) transporter complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006824">
        <property type="term" value="P:cobalt ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16914">
        <property type="entry name" value="EcfT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003339">
        <property type="entry name" value="ABC/ECF_trnsptr_transmembrane"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052770">
        <property type="entry name" value="Cobalt_transport_CbiQ"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012809">
        <property type="entry name" value="ECF_CbiQ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02454">
        <property type="entry name" value="ECF_T_CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43723">
        <property type="entry name" value="COBALT TRANSPORT PROTEIN CBIQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43723:SF1">
        <property type="entry name" value="COBALT TRANSPORT PROTEIN CBIQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02361">
        <property type="entry name" value="CbiQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="21"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="63"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="149"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="186"/>
            <end position="203"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="236"/>
            <end position="253"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96836.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="259" mass="30162" checksum="BA1CA33B45971899" modified="2013-06-26" version="1">MKYSIDTLAYNNNLKNVNPKLKVLFAILCLITSVISTSIVVPIVIFFVMSYLIIFKAKIPKKIYLYLITPAIGFGIFTLVFMSIWYGTNEIFSIPIFSFKISFHKYGLNLGLLVFSRMLGGVSSMLFLALTTPMPQLFYVLRDLKFPSVFIEMLMMIYRYIFVLLDEMIRIKFAQETRLGYKDLKTSYRSLGILAAQLFIRAFEKGEKAYLYMLSRCYDGKLRTLESIENPKLKDVLLIFIFEFILLILVFITKDIRIF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6VWV7</accession>
    <name>N6VWV7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Chemotaxis protein CheA</fullName>
            <ecNumber evidence="2">2.7.13.3</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="16" type="ORF">J422_07102</name>
    </gene>
    <organism evidence="16 17">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="16 17" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="16 17">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.</text>
            <dbReference type="EC" id="2.7.13.3"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="16">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.13.3" evidence="2"/>
    <dbReference type="EMBL" id="APMM01000074">
        <property type="protein sequence ID" value="ENN95572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594966.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000074.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VWV7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01216"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1374"/>
    <dbReference type="OrthoDB" id="293137at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000155">
        <property type="term" value="F:phosphorelay sensor kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd00731">
        <property type="entry name" value="CheA_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd16916">
        <property type="entry name" value="HATPase_CheA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00088">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.565.10:FF:000016">
        <property type="entry name" value="Chemotaxis protein CheA, putative"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.560">
        <property type="entry name" value="Histidine kinase CheA-like, homodimeric domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1110">
        <property type="entry name" value="Histidine kinase CheA-like, P2 response regulator-binding domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.565.10">
        <property type="entry name" value="Histidine kinase-like ATPase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.160">
        <property type="entry name" value="HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.40">
        <property type="entry name" value="SH3 Domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051315">
        <property type="entry name" value="Bact_Chemotaxis_CheA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004105">
        <property type="entry name" value="CheA-like_dim"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037006">
        <property type="entry name" value="CheA-like_homodim_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037052">
        <property type="entry name" value="CheA-like_P2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010808">
        <property type="entry name" value="CheA_P2-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036061">
        <property type="entry name" value="CheW-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002545">
        <property type="entry name" value="CheW-lke_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035891">
        <property type="entry name" value="CheY-binding_CheA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036890">
        <property type="entry name" value="HATPase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003594">
        <property type="entry name" value="HATPase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005467">
        <property type="entry name" value="His_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036097">
        <property type="entry name" value="HisK_dim/P_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036641">
        <property type="entry name" value="HPT_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004358">
        <property type="entry name" value="Sig_transdc_His_kin-like_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008207">
        <property type="entry name" value="Sig_transdc_His_kin_Hpt_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43395:SF1">
        <property type="entry name" value="CHEMOTAXIS PROTEIN CHEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43395">
        <property type="entry name" value="SENSOR HISTIDINE KINASE CHEA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01584">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02895">
        <property type="entry name" value="H-kinase_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02518">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01627">
        <property type="entry name" value="Hpt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07194">
        <property type="entry name" value="P2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00344">
        <property type="entry name" value="BCTRLSENSOR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00260">
        <property type="entry name" value="CheW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01231">
        <property type="entry name" value="H-kinase_dim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00387">
        <property type="entry name" value="HATPase_c"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00073">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55874">
        <property type="entry name" value="ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50341">
        <property type="entry name" value="CheW-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55052">
        <property type="entry name" value="CheY-binding domain of CheA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47226">
        <property type="entry name" value="Histidine-containing phosphotransfer domain, HPT domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47384">
        <property type="entry name" value="Homodimeric domain of signal transducing histidine kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50851">
        <property type="entry name" value="CHEW"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50109">
        <property type="entry name" value="HIS_KIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50894">
        <property type="entry name" value="HPT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0145">Chemotaxis</keyword>
    <keyword evidence="8 16" id="KW-0418">Kinase</keyword>
    <keyword evidence="7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 11" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="17" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="10" id="KW-0902">Two-component regulatory system</keyword>
    <feature type="domain" description="HPt" evidence="15">
        <location>
            <begin position="1"/>
            <end position="105"/>
        </location>
    </feature>
    <feature type="domain" description="Histidine kinase" evidence="13">
        <location>
            <begin position="615"/>
            <end position="824"/>
        </location>
    </feature>
    <feature type="domain" description="CheW-like" evidence="14">
        <location>
            <begin position="826"/>
            <end position="955"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="130"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="12">
        <location>
            <begin position="525"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="138"/>
            <end position="163"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Basic and acidic residues" evidence="12">
        <location>
            <begin position="531"/>
            <end position="556"/>
        </location>
    </feature>
    <feature type="modified residue" description="Phosphohistidine" evidence="11">
        <location>
            <position position="48"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000085"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021495"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022500"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023012"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="PROSITE" id="PS50851"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="PROSITE" id="PS50894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="EMBL" id="ENN95572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="17">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="955" mass="108894" checksum="65AE3DF346D4AFE4" modified="2013-06-26" version="1">MAEIMEEYLDVFVEEAEEHIQKINECLLELEKNPDNLNLVHEIFRSAHTIKGGARTVGLTHISELTHHMEDILDQIRNGRIPVTSEIVDLLFECLDALETMLEEVKSGEYETSIDYNAIIEKIKNMKDKYLGGAPPTEEEKKEEAKEEKKEKEETKEEKEEVKEEKKPVKKIIKIKYKSPKTKVKVKKVVKQQVEIELEEISEDISEIIEGIKKPLEIFYSLNEKLNNEYLKSILDTIKEYIDKVSSKEIKITKKVYDTLNYITKVLEDVAKCLEEGSDLKEIVNLDELKNKIEESFKTKEIKEIESVEEIEVELDHEKFDLSEFASLIKEKLESGYKLYHLKAKVEDACELKSARLAILLTRLKSVGELLLSIPTEAELKEKTFEELEAMFLSEKDVDEIKKSLEMPEIEYIAISPVDIEFEEEVIEAKEEEIVEEEPVPDAYKIEVILDEECLLRAVRAYMVIKELRELGEVVKTNPPLESIENGEFNGLRFTIYFKPKEGVEVDDIKETVMGIPEIKEVNVIPPGEQVEEKKEEAKEEKKEKEETKEEKKEEAKEKQIQQIQAAAKKESKKSTQTIRINIEKLDKLMNLVGELVITRANFAQIANKYQIKELNNAVNRLSMLINELQEEVMAMRMVPVSYVFNRFPRMVRDLAKALNKEVELIMEGTDIELDRTVLDELAEPLVHLIRNAIDHGIEPPEEREKLGKPRKGRLRLAAIRERDHVNIIVEDDGRGIDPEKVKKKAIERGLITEEEAKKMSDHEIINLIFLPGFSTKEQVSEVSGRGVGMDVVKTKIESLGGSVVVYSEKGKGTRVVLKLPLTMAIITALLMKLSDQVYAIPITSVLDVTRIEKSEVKNIEGTNAIIYRDEIIPVIWLKDFLGFSHLPDPPGDELVIVVIERSNGKLGVVVDDVIGREDIVVKTLTGMLKNIPGLAGATILGDGRVALILDLSNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VWW8</accession>
    <name>N6VWW8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable tRNA pseudouridine synthase D</fullName>
            <ecNumber evidence="3">5.4.99.27</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">tRNA pseudouridine(13) synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">tRNA pseudouridylate synthase D</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="3">tRNA-uridine isomerase D</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">truD</name>
        <name evidence="5" type="ORF">J422_07032</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Could be responsible for synthesis of pseudouridine from uracil-13 in transfer RNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>uridine(13) in tRNA = pseudouridine(13) in tRNA</text>
            <dbReference type="Rhea" id="RHEA:42540"/>
            <dbReference type="Rhea" id="RHEA-COMP:10105"/>
            <dbReference type="Rhea" id="RHEA-COMP:10106"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="EC" id="5.4.99.27"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 3">Belongs to the pseudouridine synthase TruD family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.99.27" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000068">
        <property type="protein sequence ID" value="ENN95587.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594742.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000068.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VWW8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00918"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1362"/>
    <dbReference type="OrthoDB" id="1798at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0160150">
        <property type="term" value="F:tRNA pseudouridine(13) synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031119">
        <property type="term" value="P:tRNA pseudouridine synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2350.20">
        <property type="entry name" value="TruD, catalytic domain"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01082">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020103">
        <property type="entry name" value="PsdUridine_synth_cat_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001656">
        <property type="entry name" value="PsdUridine_synth_TruD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011760">
        <property type="entry name" value="PsdUridine_synth_TruD_insert"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042214">
        <property type="entry name" value="TruD_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00094">
        <property type="entry name" value="tRNA_TruD_broad"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326:SF21">
        <property type="entry name" value="PSEUDOURIDYLATE SYNTHASE PUS7L"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13326">
        <property type="entry name" value="TRNA PSEUDOURIDINE SYNTHASE D"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01142">
        <property type="entry name" value="TruD"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037016">
        <property type="entry name" value="Pseudouridin_synth_euk_prd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55120">
        <property type="entry name" value="Pseudouridine synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50984">
        <property type="entry name" value="TRUD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="TRUD" evidence="4">
        <location>
            <begin position="206"/>
            <end position="393"/>
        </location>
    </feature>
    <feature type="active site" description="Nucleophile" evidence="3">
        <location>
            <position position="130"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS50984"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95587.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="430" mass="51288" checksum="0F2D853B38936531" modified="2013-06-26" version="1">MFNIIKERKNESRKNLLEKVKRFRLRFRDKRIENYLKSMPLNMNKYLTNLYTGGVIKKYVEDFIVEEITKDGVILEVGKSVGFKDVEDWKGNYIHFTLEKVNWSTIDAIREIANRVNKPRKHFGFAGNKDKFAITTQRVGCFNVRLEDLKRVKIKGIVLRDFQKVNWKIKLGDLWGNRFTIRVREPEVKGEKLRETLKKAKNEYKIFLNYYGIQRFGDKRPITHIVGRFIIERDWESAFHLYCGTPLPYDDDKSKFARRLIDEGEYRLAYKKFPKVFFYERKMIKAYLESDSYEKAFLILPPYLRCMFINAYQSYLFNEIINRRYEYGFEKLEGDILVDSYPSGALFGYKTKFASGLQGEIEREIYEEEGLKPEDFKIGKFGSFIGDRRAMISKIYNLTYEIEDNSYILKFCLKKGCYATSVLREFLDRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VWZ4</accession>
    <name>N6VWZ4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphatidylglycerophosphatase A</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06867</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95622.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594251.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VWZ4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01074"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1332"/>
    <dbReference type="OrthoDB" id="53211at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008962">
        <property type="term" value="F:phosphatidylglycerophosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006629">
        <property type="term" value="P:lipid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06971">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3760.10">
        <property type="entry name" value="PgpA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036681">
        <property type="entry name" value="PgpA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007686">
        <property type="entry name" value="YutG/PgpA"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04608">
        <property type="entry name" value="PgpA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF101307">
        <property type="entry name" value="YutG-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="YutG/PgpA" evidence="1">
        <location>
            <begin position="70"/>
            <end position="137"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04608"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95622.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="142" mass="16108" checksum="A1E1565BF8A6AD3F" modified="2013-06-26" version="1">MLILEKLRISLDDLINSAMELYIGEDYKKVRDKLKDIIIKSLNNPNVSTLIIAAYLLDMEGRKNKLPFNYEEDPNYVYVDEVIGLAIANEIAGTKAIFNFRFYDKEKPGVIGRLDKEGYMFLDDAIAGLLAGCMSKVFEDEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VWZ9</accession>
    <name>N6VWZ9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">D-glucuronyl C5-epimerase domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06892</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95627.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594262.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VWZ9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01069"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="9"/>
            <end position="29"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95627.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="124" mass="14766" checksum="C570A54F5181B63C" modified="2013-06-26" version="1">MLKNIKKYVLIFTVMLSLVLSFIFGYFIGISQITYSENPIIQYIKNPKPFTVENVNMPVTYYGTINGKYIGYQITPHNVNDEARKCFYKYLKLKNEDQEEAENYLKRGFIFNRISNISIRRRNR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX39</accession>
    <name>N6VX39_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Phosphoadenosine phosphosulphate reductase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06603</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000055">
        <property type="protein sequence ID" value="ENN95677.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593729.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX39"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00599"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1285"/>
    <dbReference type="OrthoDB" id="5817at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd23947">
        <property type="entry name" value="PAPS_reductase-like_YbdN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002500">
        <property type="entry name" value="PAPS_reduct_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050128">
        <property type="entry name" value="Sulfate_adenylyltrnsfr_sub2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006327">
        <property type="entry name" value="PRK08557.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43196:SF2">
        <property type="entry name" value="PHOSPHOADENOSINE PHOSPHOSULFATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43196">
        <property type="entry name" value="SULFATE ADENYLYLTRANSFERASE SUBUNIT 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01507">
        <property type="entry name" value="PAPS_reduct"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphoadenosine phosphosulphate reductase" evidence="1">
        <location>
            <begin position="10"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01507"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95677.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="230" mass="27727" checksum="5FD532D42FEFC8E6" modified="2013-06-26" version="1" fragment="single">FIEKYKNKNINASFSGGKDSSVSTLLAKKVIKDIEVIFIDTGLEFPETLKFVNKFAKEYDINLTILKGKNFWKYLKYYGIPTKDNRWCNSICKLEPLKEYLRKYKTVYTVDGSRKFESFSREKLSYERKSRFIKNQINIFPILYWKATDVWSWIYLNDIIYNPLYDRGFERIGCYLCPSMLSAEFLIVKQIYPELFYKWFNVLKSYGYSEEEILKGFWRWKKLPKKMKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX49</accession>
    <name>N6VX49_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">AMP phosphorylase</fullName>
            <shortName evidence="3">AMPpase</shortName>
            <ecNumber evidence="3">2.4.2.57</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Nucleoside monophosphate phosphorylase</fullName>
            <shortName evidence="3">NMP phosphorylase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_06341</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of AMP and phosphate to adenine and ribose 1,5-bisphosphate (R15P). Exhibits phosphorylase activity toward CMP and UMP in addition to AMP. Functions in an archaeal AMP degradation pathway, together with R15P isomerase and RubisCO.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>AMP + phosphate = alpha-D-ribose 1,5-bisphosphate + adenine</text>
            <dbReference type="Rhea" id="RHEA:36975"/>
            <dbReference type="ChEBI" id="CHEBI:16708"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.4.2.57"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>CMP + phosphate = cytosine + alpha-D-ribose 1,5-bisphosphate</text>
            <dbReference type="Rhea" id="RHEA:36987"/>
            <dbReference type="ChEBI" id="CHEBI:16040"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="EC" id="2.4.2.57"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>UMP + phosphate = alpha-D-ribose 1,5-bisphosphate + uracil</text>
            <dbReference type="Rhea" id="RHEA:36991"/>
            <dbReference type="ChEBI" id="CHEBI:17568"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57865"/>
            <dbReference type="ChEBI" id="CHEBI:68688"/>
            <dbReference type="EC" id="2.4.2.57"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the thymidine/pyrimidine-nucleoside phosphorylase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.4.2.57" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95687.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593508.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX49"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01305"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1234"/>
    <dbReference type="OrthoDB" id="9827at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004645">
        <property type="term" value="F:1,4-alpha-oligoglucan phosphorylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016208">
        <property type="term" value="F:AMP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016763">
        <property type="term" value="F:pentosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006196">
        <property type="term" value="P:AMP catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046125">
        <property type="term" value="P:pyrimidine deoxyribonucleoside metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006206">
        <property type="term" value="P:pyrimidine nucleobase metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.970.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.40.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1030.10">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.30">
        <property type="entry name" value="Pyrimidine nucleoside phosphorylase-like, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02132">
        <property type="entry name" value="AMP_phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017713">
        <property type="entry name" value="AMP_phosphorylase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000312">
        <property type="entry name" value="Glycosyl_Trfase_fam3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017459">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036320">
        <property type="entry name" value="Glycosyl_Trfase_fam3_N_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035902">
        <property type="entry name" value="Nuc_phospho_transferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036566">
        <property type="entry name" value="PYNP-like_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013102">
        <property type="entry name" value="PYNP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017872">
        <property type="entry name" value="Pyrmidine_PPase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013466">
        <property type="entry name" value="Thymidine/AMP_Pase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000053">
        <property type="entry name" value="Thymidine/pyrmidine_PPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03327">
        <property type="entry name" value="AMP_phos"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02645">
        <property type="entry name" value="ARCH_P_rylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003338">
        <property type="entry name" value="PRK04350.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10515">
        <property type="entry name" value="THYMIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10515:SF0">
        <property type="entry name" value="THYMIDINE PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02885">
        <property type="entry name" value="Glycos_trans_3N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00591">
        <property type="entry name" value="Glycos_transf_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07831">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000478">
        <property type="entry name" value="TP_PyNP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00941">
        <property type="entry name" value="PYNP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52418">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47648">
        <property type="entry name" value="Nucleoside phosphorylase/phosphoribosyltransferase N-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54680">
        <property type="entry name" value="Pyrimidine nucleoside phosphorylase C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00647">
        <property type="entry name" value="THYMID_PHOSPHORYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 3 5" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 3 5" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyrimidine nucleoside phosphorylase C-terminal" evidence="4">
        <location>
            <begin position="422"/>
            <end position="489"/>
        </location>
    </feature>
    <feature type="active site" description="Proton donor" evidence="3">
        <location>
            <position position="256"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="168"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="194"/>
            <end position="199"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="203"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <position position="286"/>
        </location>
        <ligand>
            <name>AMP</name>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02132"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95687.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="501" mass="55151" checksum="05505BE895E44AE8" modified="2013-06-26" version="1">MLFLKAKILDIDLENIVLINYDDLKNSQYFSQDRVVIEYKNKEIIAFLYSSKDLINSGEIGLPKKIADELKVKEGDIVNVSHADKPISIQYIKKKMDGNKLKKEEIYTIIDEIVDNNLSNIEISAFITALYINGMDIEEITSMTIRMAETGEMIKWEGQVYDVHSIGGVPGNKYALITVPIIAYTGLKIPKTSSRAITSAAGTADIVEVLTRVNLKVEEIKRVVKETNGCLVWGGALELAPADDITITVERALNIDPKPLLLSSVLSKKLAMGINKLLIDIPLGAKVKNMDEASSLARNFIELGDRLNIYVDCAITYGEQPIGRAIGPALEAKEALLALEDYKKAPNSLVEKAISLSGILLEMGGIAPKGEGKDLAEDILATGKAHDKFMEIIVAQGGEEKTSDEIEVGKYYTDILSPIDGYVTKISNEKITKIAKTAGAPNDKKAGVYLNVKVGNKVEKNDVLFTIYSDSKERLEKAVKLSRLLMPVKVEGMLLKRITRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX76</accession>
    <name>N6VX76_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Metal dependent phosphohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06296</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000051">
        <property type="protein sequence ID" value="ENN95717.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX76"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="38"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95717.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="38" mass="4382" checksum="DBD33EBF19987E97" modified="2013-06-26" version="1" fragment="single">MDALELLKKLCPKNVVEHCIAVSKYAYEIALQIKNNDY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXD2</accession>
    <name>N6VXD2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05968</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95782.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593045.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXD2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00565"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1162"/>
    <dbReference type="OrthoDB" id="65991at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007509">
        <property type="entry name" value="DUF515"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04415">
        <property type="entry name" value="DUF515"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="44"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95782.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="384" mass="44038" checksum="DF6147BE36144CFC" modified="2013-06-26" version="1">MVDPDKIKKLKERSKKSIKSANITHYLLIIIIVIFGVFTVYIVYSNMASQKEKTVPVEIGINLEKQKTSAINSLNQMFSKYPDDPMLGVYRNEILSATSPNEIKDVLNRAAEYIKLKEYKENIKNQIKNICGDFYYQSKYALKMVGEIDKAKTIDEISNIYNKYYPYLYNEIRDFYVNKYITEYSQAQYLRVISDGNERLYSYIEFVDFIKNLDLESLKKLKVYEVKMVYIILPILAKNCGDIPKPGSKVIIYKLSGKNSSKITEGIITKGYIIVPDISYSESSSKSVSLNDNGETESASETKSVTYSLKGINFILHSAVIGKLNYYEVLQKLKNYGYRLNEIEDKTGIFDDKLVYLLELKVPSDKVNNLLGLSDKEVAIAKIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VY83</accession>
    <name>N6VY83_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_04303</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the ABC transporter superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96062.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591752.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VY83"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00663"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.843"/>
    <dbReference type="OrthoDB" id="24644at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03214">
        <property type="entry name" value="ABC_Iron-Siderophores_B12_Hemin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000134">
        <property type="entry name" value="Iron-enterobactin ABC transporter ATP-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050153">
        <property type="entry name" value="Metal_Ion_Import_ABC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42734">
        <property type="entry name" value="METAL TRANSPORT SYSTEM ATP-BINDING PROTEIN TM_0124-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42734:SF6">
        <property type="entry name" value="MOLYBDATE IMPORT ATP-BINDING PROTEIN MOLC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="5">
        <location>
            <begin position="3"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005417"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96062.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="250" mass="28615" checksum="4B2CE3E4DC5D0B63" modified="2013-06-26" version="1">MILSVDNIKFSYKSREVLKGVTFKVKRGEVASILGENGSGKSTLLKCINKILKPKIGSIFIEKYNINELNSKELSKKIAYVPQKSNANYITVFDAVLLGRKPYIKFDVSDEDIKVVEEVLKLMELEDIAFRYINELSGGELQRVVIARALAQEPRVLLLDEPTNNLDLRYQLKILKLIREITKIKNIATIMVMHDINLALRFSDKFLFMKDGIIYYEGEKNSITPEIIEEVYGIKVYIGYYNNTPVVIPI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYB4</accession>
    <name>N6VYB4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 8">DNA repair and recombination protein RadA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">radA</name>
        <name evidence="10" type="ORF">J422_04523</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the eukaryotic RecA-like protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96102.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYB4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00620"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.887"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140664">
        <property type="term" value="F:ATP-dependent DNA damage sensor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003684">
        <property type="term" value="F:damaged DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.20">
        <property type="entry name" value="5' to 3' exonuclease, C-terminal subdomain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011938">
        <property type="entry name" value="DNA_recomb/repair_RadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010995">
        <property type="entry name" value="DNA_repair_Rad51/TF_NusA_a-hlx"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003583">
        <property type="entry name" value="Hlx-hairpin-Hlx_DNA-bd_motif"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013632">
        <property type="entry name" value="Rad51_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020588">
        <property type="entry name" value="RecA_ATP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003301">
        <property type="entry name" value="PRK04301.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02236">
        <property type="entry name" value="recomb_radA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942:SF30">
        <property type="entry name" value="MEIOTIC RECOMBINATION PROTEIN DMC1_LIM15 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22942">
        <property type="entry name" value="RECA/RAD51/RADA DNA STRAND-PAIRING FAMILY MEMBER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14520">
        <property type="entry name" value="HHH_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08423">
        <property type="entry name" value="Rad51"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00278">
        <property type="entry name" value="HhH1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47794">
        <property type="entry name" value="Rad51 N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50162">
        <property type="entry name" value="RECA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0227">DNA damage</keyword>
    <keyword evidence="7" id="KW-0233">DNA recombination</keyword>
    <keyword evidence="6" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RecA family profile 1" evidence="9">
        <location>
            <begin position="75"/>
            <end position="256"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008050"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00018144"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="NCBIfam" id="TIGR02236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50162"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96102.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="275" mass="30497" checksum="D272CDEC334D66C4" modified="2013-06-26" version="1">MDDLTKLPGVGPSMAEKLKEAGYTDFMKIAIATVEELSEIEGISEKAAAKIIEAAKKFCDLGFKTGTEILKQRGYIWKLSTGSKNLDEILGGGLESQSVTEFAGMFGSGKTQIAHQACVNLQCPDKIIADKEIIKEEFKDPKAVYIDTEGTFRPERIIQMAEALGLDGQKVLDSIFYARAYNSDMQMLFAENVENLIKEGHNIKLIVIDSLTSAFRTEYIGRGKLAERQQKLGRHMATLNRLADIYNCVVLVTNQVAARLMLSLEHLSKLLEDIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYC1</accession>
    <name>N6VYC1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">DNA topoisomerase type IA zn finger domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04098</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000027">
        <property type="protein sequence ID" value="ENN96112.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591612.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYC1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01355"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.802"/>
    <dbReference type="OrthoDB" id="190320at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005694">
        <property type="term" value="C:chromosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003916">
        <property type="term" value="F:DNA topoisomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006265">
        <property type="term" value="P:DNA topological change"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.65.10">
        <property type="entry name" value="Bacterial Topoisomerase I, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013498">
        <property type="entry name" value="Topo_IA_Znf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01396">
        <property type="entry name" value="Zn_ribbon_Top1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0413">Isomerase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DNA topoisomerase type IA zn finger" evidence="1">
        <location>
            <begin position="246"/>
            <end position="282"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01396"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96112.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="284" mass="33574" checksum="ECEC999116ADD7E8" modified="2013-06-26" version="1">MIELFNILSKDIHINITKLSDETFNQNWKVPEGFISIIGLENAKLPVYYYGITNNYEKYYDIKKVETPKGKQIFVRLYYQFDRRDIIEKEKYVVANAFTGLLLLIKIDKEEFIENATKLLKHVYKKDEFVKDTLDSINLFDDLDETIRFVANRDYNWLIGRIKYNMGLLKSSGQGYYLLPVKTNMQITEGLNVNDNKLIIDLENANLFKHYIIYVDTVNNKIFFNRNRLCNKKPILEEIMSKIDENTCPWCGAKLRVVKTKKGEFLGCSNYPKCLYRRFPNKDE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYC5</accession>
    <name>N6VYC5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF35 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_04123</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000027">
        <property type="protein sequence ID" value="ENN96117.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591623.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYC5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01350"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.807"/>
    <dbReference type="OrthoDB" id="9573at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.30.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002878">
        <property type="entry name" value="ChsH2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022002">
        <property type="entry name" value="ChsH2_Znr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052513">
        <property type="entry name" value="Thioester_dehydratase-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34075:SF5">
        <property type="entry name" value="BLR3430 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01796">
        <property type="entry name" value="OB_ChsH2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12172">
        <property type="entry name" value="zf-ChsH2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ChsH2 rubredoxin-like zinc ribbon" evidence="2">
        <location>
            <begin position="9"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="domain" description="ChsH2 C-terminal OB-fold" evidence="1">
        <location>
            <begin position="47"/>
            <end position="109"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01796"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF12172"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96117.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="130" mass="15111" checksum="CB5E0962D7D95FF6" modified="2013-06-26" version="1">MVVRSWRHIKERYNLIGVKCLNCNTVYFPTRELCPKCRRKTKFEEIKLSGRGKVYSYSIIHTAPKEFEKEAPYIIAIVELEEGVKVTGQIVDCPLDKIKIGLPVETVFRKIKEEGEDGVIVYGYKFRPIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYH0</accession>
    <name>N6VYH0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">4Fe-4S ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_03893</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96167.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591381.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYH0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00409"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.763"/>
    <dbReference type="OrthoDB" id="5583at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF5">
        <property type="entry name" value="4FE-4S FERREDOXIN-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="1"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="32"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96167.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="63" mass="6889" checksum="E7B48FA4BBB037F0" modified="2013-06-26" version="1">MGVTIDYSICRGAECAECYNNCPMEVFEIEGDKVVVAREEDCSYCGVCEDVCPTGAVKVEPEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYR5</accession>
    <name>N6VYR5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03398</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96272.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590992.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYR5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01065"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.667"/>
    <dbReference type="OrthoDB" id="64141at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96272.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="84" mass="9722" checksum="AF96930C8F3A84FB" modified="2013-06-26" version="1">MPSEKIQEILNELNNIMNVEKKYIELVATVEYLLNLIEPSKREKFREALNDAESVEDVYELIKAIKLQLGIQGAKKYVLKIEEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYY2</accession>
    <name>N6VYY2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Succinyl-CoA synthetase subunit beta</fullName>
            <ecNumber evidence="8">6.2.1.5</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">sucC</name>
        <name evidence="8" type="ORF">J422_02614</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.2.1.5" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96337.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590553.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYY2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00075"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.512"/>
    <dbReference type="OrthoDB" id="146449at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042709">
        <property type="term" value="C:succinate-CoA ligase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004775">
        <property type="term" value="F:succinate-CoA ligase (ADP-forming) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006104">
        <property type="term" value="P:succinyl-CoA metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000002">
        <property type="entry name" value="Succinate--CoA ligase [ADP-forming] subunit beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.261">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013650">
        <property type="entry name" value="ATP-grasp_succ-CoA_synth-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017866">
        <property type="entry name" value="Succ-CoA_synthase_bsu_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005811">
        <property type="entry name" value="SUCC_ACL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005809">
        <property type="entry name" value="Succ_CoA_ligase-like_bsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016102">
        <property type="entry name" value="Succinyl-CoA_synth-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001913">
        <property type="entry name" value="PRK00696.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01016">
        <property type="entry name" value="sucCoAbeta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815:SF10">
        <property type="entry name" value="SUCCINATE--COA LIGASE [GDP-FORMING] SUBUNIT BETA, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11815">
        <property type="entry name" value="SUCCINYL-COA SYNTHETASE BETA CHAIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08442">
        <property type="entry name" value="ATP-grasp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00549">
        <property type="entry name" value="Ligase_CoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001554">
        <property type="entry name" value="SucCS_beta"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52210">
        <property type="entry name" value="Succinyl-CoA synthetase domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01217">
        <property type="entry name" value="SUCCINYL_COA_LIG_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8" id="KW-0436">Ligase</keyword>
    <keyword evidence="5" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="7">
        <location>
            <begin position="9"/>
            <end position="217"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96337.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="361" mass="40947" checksum="010578C2D95B5EED" modified="2013-06-26" version="1">MKLYEYEAKNLFRYYNIPVPESFLIYKDDNLDLVDIDKEVVLKAQVLVGGRGKAGGILFAKNKKEFIEKAKELFNKEIKGEKVEKILVEEKLKIKKEYYVSITIDRDAKKPVLIFSTEGGVDIEEVAKKNPEKIIKYHIDVLKPFLPYKIRWILDGKIPSEELAIANIIYNLYQLFKEKDATLVEINPLVITEDNKIYAADAVVILDDDAHYRQNFEAFEESNKEKLPFAYVELDGDITVIGNGAGLTLASMDIIKNLGREPACFLDIGGGADTETVKLALKKVLSNKKVKGVFINILAGITKCDEVAKGIVEILKEHPNVKFSVRMMGTNEELGRKILEENNIYYETSMEEAAKKLIENL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZH9</accession>
    <name>N6VZH9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Glutamyl-tRNA(Gln) amidotransferase subunit A</fullName>
            <shortName evidence="7">Glu-ADT subunit A</shortName>
            <ecNumber evidence="7">6.3.5.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7 9" type="primary">gatA</name>
        <name evidence="9" type="ORF">J422_01850</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>L-glutamyl-tRNA(Gln) + L-glutamine + ATP + H2O = L-glutaminyl-tRNA(Gln) + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17521"/>
            <dbReference type="Rhea" id="RHEA-COMP:9681"/>
            <dbReference type="Rhea" id="RHEA-COMP:9684"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:78520"/>
            <dbReference type="ChEBI" id="CHEBI:78521"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.7"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterotrimer of A, B and C subunits.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7">Belongs to the amidase family. GatA subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.7" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96517.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590124.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZH9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00218"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.364"/>
    <dbReference type="OrthoDB" id="7931at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030956">
        <property type="term" value="C:glutamyl-tRNA(Gln) amidotransferase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050567">
        <property type="term" value="F:glutaminyl-tRNA synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1300.10">
        <property type="entry name" value="Amidase signature (AS) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00120">
        <property type="entry name" value="GatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000120">
        <property type="entry name" value="Amidase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020556">
        <property type="entry name" value="Amidase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023631">
        <property type="entry name" value="Amidase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036928">
        <property type="entry name" value="AS_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004412">
        <property type="entry name" value="GatA"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00132">
        <property type="entry name" value="gatA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895:SF7">
        <property type="entry name" value="GLUTAMYL-TRNA(GLN) AMIDOTRANSFERASE SUBUNIT A, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11895">
        <property type="entry name" value="TRANSAMIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01425">
        <property type="entry name" value="Amidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75304">
        <property type="entry name" value="Amidase signature (AS) enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00571">
        <property type="entry name" value="AMIDASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 7 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 7" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Amidase" evidence="8">
        <location>
            <begin position="5"/>
            <end position="421"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="56"/>
        </location>
    </feature>
    <feature type="active site" description="Charge relay system" evidence="7">
        <location>
            <position position="131"/>
        </location>
    </feature>
    <feature type="active site" description="Acyl-ester intermediate" evidence="7">
        <location>
            <position position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008069"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00047407"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF01425"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96517.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="432" mass="48462" checksum="FDBED9FA777995FC" modified="2013-06-26" version="1">MLIEKVEEYLEKIEKNKDINAFIDIRKEKVLEEAKRLEKDEKAKKKPLYGKLIAVKANINVEGYTISCASKTLENYISPYDATVIEKIKENGGLIIGITNMDEFACGSSGETSYYGPTKNPKAKDRIPGGSSSGSAAAVAADLCDFALGSDTGGSIRNPASHCGVVGFKPSYGVVSRYGLCDLAMSFDQIGPITKNAKDALEIMNIIKGKDEKDTTTVNIKDFEVREYKPKVGIVKEFMDVTDKKIREKIERAIEVFKDMNCEIVELSYKYLDLALPTYYLINYVEFYSATRRYDGRRYGYKIEDVCGEEVLRRITIGSIISQKEYSGKYYKKALKARNLLKREYMKMMKNIDIILSPTVPKLPHKLGEKISPIEMYNYDILTVIANLVGAPAGVVPCGEINNIPVGLQIQGKPFEDEKVLNAMIMFEKNFC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZK4</accession>
    <name>N6VZK4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01960</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96537.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590156.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZK4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00239"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.385"/>
    <dbReference type="OrthoDB" id="9180at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd07709">
        <property type="entry name" value="flavodiiron_proteins_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045761">
        <property type="entry name" value="ODP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43041">
        <property type="entry name" value="HYDROLASE, METALLO-BETA-LACTAMASE SUPERFAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43041:SF1">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19583">
        <property type="entry name" value="ODP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="41"/>
            <end position="230"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96537.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="257" mass="29308" checksum="02D05AC080EE66A7" modified="2013-06-26" version="1">MDGEYFIKPGLDPKKDHILYRDKEHKVIYLGTLGSGKGDVDVISYLIVNKGRGFLVDPGGYNIFPKVLSNISKYINPKNIDYIYMCHQDPDVAGSVPLWRSITNAKLVTSWLWIRFLPHFGFEDVDTVAYPLPDEGETLKFGSTTLEFIPAHFLHSPGHFSIYDRRSKFLFTGDIGIALPEKPILVVEDIEKHIEAMRPIHERLMPTSLAIKNWLNRVRGLDIEAILPQHGGIIQKKDVPKFFRFLDSLKCGVEVMK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6W070</accession>
    <name>N6W070_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">XRE family transcriptional regulator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00531</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96757.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589788.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W070"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00058"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.102"/>
    <dbReference type="OrthoDB" id="59250at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00093">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.260.40">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001387">
        <property type="entry name" value="Cro/C1-type_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010982">
        <property type="entry name" value="Lambda_DNA-bd_dom_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01381">
        <property type="entry name" value="HTH_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00530">
        <property type="entry name" value="HTH_XRE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47413">
        <property type="entry name" value="lambda repressor-like DNA-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50943">
        <property type="entry name" value="HTH_CROC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH cro/C1-type" evidence="1">
        <location>
            <begin position="19"/>
            <end position="55"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50943"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96757.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="210" mass="24307" checksum="A70B1C7D5FC37443" modified="2013-06-26" version="1">MFKKLEIIERAILLNPRYIQSFRKKLKITQSRLAKESGISQSHLSMLEKGKREASELIAAAITYGLLKCFSEKSKNPIIELLDTLSLLKFEDAFAELVNNIIKENDIKYIRNIDGFPVIIIDKDSLINEMKKRLKVINLEKIEISRGKIKAFGKHIDSYVDIILDCSDIRRLEKKCSEKTKKNIIIQSFPKEEVPPIYGVKRDCVIIHCW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6W0B1</accession>
    <name>N6W0B1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YqhA family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00120</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96807.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589713.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W0B1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01144"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.24"/>
    <dbReference type="OrthoDB" id="111817at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005134">
        <property type="entry name" value="UPF0114"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31721">
        <property type="entry name" value="OS06G0710300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31721:SF4">
        <property type="entry name" value="OS06G0710300 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03350">
        <property type="entry name" value="UPF0114"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF026509">
        <property type="entry name" value="UCP026509"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="21"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="70"/>
            <end position="94"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="129"/>
            <end position="147"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="153"/>
            <end position="170"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96807.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="173" mass="19678" checksum="03A65796899EE1DF" modified="2013-06-26" version="1">MDNKKIKKIELLFESFLWKSKVVSILAVLFSLLGAIMIFLIASYEIIRIFPKVILYLFGYYVKNFHDQLIGTVISAIDLYLIAIVLLIFSFGIYELFISKIDIAECSEIGNKILVINNLDDLKDKLGKVVILALIVGIFKKILSIGIKNTLDVLLMSISIITLSISLYYLHKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6W0B8</accession>
    <name>N6W0B8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Putative adenylate kinase</fullName>
            <shortName evidence="7">AK</shortName>
            <ecNumber evidence="7">2.7.4.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">ATP-AMP transphosphorylase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_00170</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Broad-specificity nucleoside monophosphate (NMP) kinase that catalyzes the reversible transfer of the terminal phosphate group between nucleoside triphosphates and monophosphates. Has also ATPase activity. Involved in the late maturation steps of the 30S ribosomal particles, specifically 16S rRNA maturation. While NMP activity is not required for ribosome maturation, ATPase activity is. Associates transiently with small ribosomal subunit protein uS11. ATP hydrolysis breaks the interaction with uS11. May temporarily remove uS11 from the ribosome to enable a conformational change of the ribosomal RNA that is needed for the final maturation step of the small ribosomal subunit.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>AMP + ATP = 2 ADP</text>
            <dbReference type="Rhea" id="RHEA:12973"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>ATP + H2O = ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13065"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="7">Interacts with uS11. Not a structural component of 40S pre-ribosomes, but transiently interacts with them by binding to uS11.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the adenylate kinase family. AK6 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.3" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96817.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589723.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W0B8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01134"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.34"/>
    <dbReference type="OrthoDB" id="8730at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004017">
        <property type="term" value="F:AMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042274">
        <property type="term" value="P:ribosomal small subunit biogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006364">
        <property type="term" value="P:rRNA processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00039">
        <property type="entry name" value="Adenylate_kinase_AK6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020618">
        <property type="entry name" value="Adenyl_kinase_AK6"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003012">
        <property type="entry name" value="PRK03839.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595:SF0">
        <property type="entry name" value="ADENYLATE KINASE ISOENZYME 6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12595">
        <property type="entry name" value="POS9-ACTIVATING FACTOR FAP7-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13238">
        <property type="entry name" value="AAA_18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 7" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5 7 8" id="KW-0418">Kinase</keyword>
    <keyword evidence="4 7" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 7" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="2 7" id="KW-0698">rRNA processing</keyword>
    <keyword evidence="3 7" id="KW-0808">Transferase</keyword>
    <feature type="region of interest" description="LID" evidence="7">
        <location>
            <begin position="99"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="10"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="12"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="13"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="14"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00039"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96817.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="170" mass="19879" checksum="A7B5B203272B552F" modified="2013-06-26" version="1">MRIAITGTPGVGKTTVSKVLAKRLNMEVIDITDVVKRYRLYIDYDEDMESYVIDFEKLNNFLKDKDNIILDGHVSHLLNPDYTIVLRCDPKIIEERLRKRGYKDKKVLENVQAEILDVCLCESKGKVYEIDTTNRDVEDIVNEIIEAIKEGKERKGIVDWLSKYYEYLEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTQ0</accession>
    <name>N6UTQ0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Type II secretion system F domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_06496</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95714.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593559.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTQ0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01276"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1264"/>
    <dbReference type="OrthoDB" id="66029at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.81.30">
        <property type="entry name" value="Type II secretion system (T2SS), domain F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056569">
        <property type="entry name" value="ArlJ-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018076">
        <property type="entry name" value="T2SS_GspF_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042094">
        <property type="entry name" value="T2SS_GspF_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402">
        <property type="entry name" value="INTEGRAL MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35402:SF1">
        <property type="entry name" value="TYPE II SECRETION SYSTEM PROTEIN GSPF DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00482">
        <property type="entry name" value="T2SSF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="106"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="130"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="275"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="309"/>
            <end position="328"/>
        </location>
    </feature>
    <feature type="domain" description="Type II secretion system protein GspF" evidence="7">
        <location>
            <begin position="171"/>
            <end position="293"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF00482"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95714.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="333" mass="37913" checksum="DB315D8E6098EE59" modified="2013-06-26" version="1">MINLKNIYIKIRLFLHNIGLITLDTATLKELKKIEVSKPKFQEAYTEPVEIEPENIEEYERILFEEELIAKSAESLSQALRNVRIFSRNLIRYLGYENEVEYFKRVVLYMFLSIFGFIILGILSKNIIGGFIKGIVGAVIILVGSIFYPRLKLMLFKGEIKLQILFTLVFMISLLRAGASLPEVLNHISKSREFGIVAYEIRQVLRDVNSGYSMLEALERAKIRTEIPLMIVFFDQIIAGYNKGNLSLLLEKLYEEIVRSSLAKLDSSKFMIQNLGNLTFGIGLIIPFSGMILSGMISNQGFPGILNTLDLLMTKIGPMLALFFGIFVKMKIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTT2</accession>
    <name>N6UTT2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06130</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95754.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593251.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTT2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00545"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1194"/>
    <dbReference type="OrthoDB" id="65927at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="26"/>
            <end position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95754.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="171" mass="20307" checksum="D36CC50375131ED7" modified="2013-06-26" version="1">MDENKQKLHYIIYLLTRDDKKWVRQIVVFALIYYFIKLKVLSYTYAPMPYIWQDKIKFINISYDALEDLNFLLDNGYISEILLSIKGLNEFIVGYKIGKSLNYNFSEKDKEIIEKTILDENGNLKEIIITENGIKIKSKEGDLDIILTELERIKYKSKSYIMKVSLWDIKP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="18" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTU0</accession>
    <name>N6UTU0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Putative cytosolic protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06180</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95764.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593271.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTU0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00555"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1204"/>
    <dbReference type="OrthoDB" id="63139at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20095">
        <property type="entry name" value="DUF6485"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95764.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="67" mass="7939" checksum="2B0D066EFCB623C5" modified="2013-06-26" version="1">MECQKEKNLERCNCTYPSCSKKGMCCECLHYHLKNRQLPACCFPPEIEKTYDRSFETFAKLVLEKKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU04</accession>
    <name>N6UU04_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flagella protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_05843</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95834.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU04"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01237"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1139"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006752">
        <property type="entry name" value="Arch_fla_DE"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052494">
        <property type="entry name" value="Flagella_assembly_related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40698:SF1">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN D-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40698">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN E-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04659">
        <property type="entry name" value="Arch_fla_DE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0974">Archaeal flagellum</keyword>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Archaeal flagella protein FlaD/E" evidence="3">
        <location>
            <begin position="26"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF04659"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95834.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="145" mass="16786" checksum="1AD07726AEA4F50A" modified="2013-06-26" version="1">MEFNELKEVKIMDALTNAILNVHKPSKLEDISYDDPVTIILAFKWLEYLCERVGVENVPDVLDFYYMLGWIGDKAMAKLLRFLKGIKVDEENVVEGSGKLNITDHIISLVFIEKLNGKQISMDFLDKIEWELRKIKKGAEQFYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU59</accession>
    <name>N6UU59_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Zinc/iron permease</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_05389</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000038">
        <property type="protein sequence ID" value="ENN95889.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592444.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000038.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU59"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00460"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1052"/>
    <dbReference type="OrthoDB" id="62226at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046873">
        <property type="term" value="F:metal ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003689">
        <property type="entry name" value="ZIP"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02535">
        <property type="entry name" value="Zip"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="36"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="59"/>
            <end position="79"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="100"/>
            <end position="121"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="157"/>
            <end position="179"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="185"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="211"/>
            <end position="228"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95889.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="8">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="229" mass="25906" checksum="6D884033D0E6BE4A" modified="2013-06-26" version="1">MLVVIYIAILSFFIMLIGSVLAYYSISLKYRKEVEAISLGFIFGVATLILLVKGYYKTFFLPLSLGILTVYIIEKFLAYCPMSKNYCVECKNLEMFKVKYIYPISFFIHTFIDGLILAISYLGNIGLPLYLAIITHKIPAGFVLLSPLKWVYKQPLLICSIISFGTVLGTILGLILLKNLPIKPLLSYSAGIFLGTFITLYPHMYHHKEDVILYIIIGYIIVGLIGMVR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUF1</accession>
    <name>N6UUF1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Enolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04985</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95969.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592109.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUF1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00492"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.977"/>
    <dbReference type="OrthoDB" id="59929at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029017">
        <property type="entry name" value="Enolase-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020811">
        <property type="entry name" value="Enolase_N"/>
    </dbReference>
    <dbReference type="SMART" id="SM01193">
        <property type="entry name" value="Enolase_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54826">
        <property type="entry name" value="Enolase N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Enolase N-terminal" evidence="1">
        <location>
            <begin position="5"/>
            <end position="102"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM01193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95969.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="307" mass="34857" checksum="0BE133C685327861" modified="2013-06-26" version="1">MLDIIRKISAKKVFKGDIKVMITTITENSFGYEIIDTEEPDNVIAEINNVIAPELFGYSLDIDLIDNIICEVSENSLISYGVSVSVARAAANSFDIPLFKYLGGAITTDLPIVSSTILRDNNSELFPIVMAESIEDIVDIYIKLRNILDYKVIDINGAYVCKDIFNEIGKVREKIDEIKEEEDIEILLGLKIKKDMVKDKDLSLVDYLEVEEPVEFDGFLCTDIIYEESDFVKVYPYEIGTVTELFYYINYIFDKGLYPVLEGDNNSLAHIATAFKVPVIRAHLSSSVLNEVWEIERSISNPNIRRF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUH1</accession>
    <name>N6UUH1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">UPF0056 membrane protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_05085</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 7">Cell membrane</location>
            <topology evidence="1 7">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the UPF0056 (MarC) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95989.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592150.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUH1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00472"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.997"/>
    <dbReference type="OrthoDB" id="10856at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002771">
        <property type="entry name" value="Multi_antbiot-R_MarC"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00427">
        <property type="entry name" value="NAAT family transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33508:SF1">
        <property type="entry name" value="UPF0056 MEMBRANE PROTEIN YHCE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01914">
        <property type="entry name" value="MarC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="33"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="45"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="113"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="142"/>
            <end position="165"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00009784"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU362048"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95989.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="211" mass="23716" checksum="6F4BDB3B43D4DF9F" modified="2013-06-26" version="1">MDILNFYIYSFVSLFITVDPIGLIPIIHSLIYPYSTYRKRIIKKAILASTTILLIFALFGNFIFSYFGITVDAFRVAGGILLFKIAWDMLHAEIPKTKHKPEEKIELEDIDSIVYVPLAIPLISGPGAITTTVILISKAQSLIYKIIVISAILTTMLVSAVIFYLSDYIIKKFNIYGINAFVRIMGLLLASISVQIIFFGLYGLYKTILLT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUR3</accession>
    <name>N6UUR3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Glyceraldehyde 3-phosphate phosphatase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_04338</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the dephosphorylation of D,L-glyceraldehyde 3-phosphate in vitro.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96069.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591768.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUR3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00655"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.850"/>
    <dbReference type="OrthoDB" id="27736at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044281">
        <property type="term" value="P:small molecule metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.150.520">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051400">
        <property type="entry name" value="HAD-like_hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011950">
        <property type="entry name" value="HAD-SF_hydro_IA_CTE7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041492">
        <property type="entry name" value="HAD_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02253">
        <property type="entry name" value="CTE7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01509">
        <property type="entry name" value="HAD-SF-IA-v3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470:SF2">
        <property type="entry name" value="GLYCERALDEHYDE 3-PHOSPHATE PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46470">
        <property type="entry name" value="N-ACYLNEURAMINATE-9-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13419">
        <property type="entry name" value="HAD_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00413">
        <property type="entry name" value="HADHALOGNASE"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01129">
        <property type="entry name" value="C1.5:_HAD__Beta-PGM__Phosphata"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00003">
        <property type="entry name" value="Haloacid_Dehalogenase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="5" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00003513"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00019531"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96069.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="227" mass="26014" checksum="67C572948B105AB4" modified="2013-06-26" version="1">MIKGILFDLDDTLYNSSEFVNIARREAVKSMIDAGLDIEFEEAMKILDKIIKDKGSNYGKHFDDLVKAVLGKYDPKIIATGIITYHNVKVALLRPYPHTIKTLMELKAMGLKLGVLTDGLTIKQWEKLIRLGIHPFFDEVITSEEFGLGKPHLEFFKYGLKRMNLKPEEVIYVGDRVDKDIIPAKKLGIISVRIIQGKYKDMEDNISDYKIKNLTELIDIIKELNKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUR7</accession>
    <name>N6UUR7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04363</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96074.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591778.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUR7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00650"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.855"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019707">
        <property type="entry name" value="DUF2582"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10771">
        <property type="entry name" value="DUF2582"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96074.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="56" mass="6347" checksum="A9193606BA1E76B1" modified="2013-06-26" version="1">MENMWAKIGETAGAIYNLLKEKGDMSLSKLENSLRKKGYNSNIIKMAVGWLARKIM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV86</accession>
    <name>N6UV86_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">C/D box methylation guide ribonucleoprotein complex aNOP56 subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_03408</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the NOP5/NOP56 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96274.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV86"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01067"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031428">
        <property type="term" value="C:box C/D methylation guide snoRNP complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030515">
        <property type="term" value="F:snoRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.220">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.660">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029012">
        <property type="entry name" value="Helix_hairpin_bin_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048895">
        <property type="entry name" value="MJ0694-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045056">
        <property type="entry name" value="Nop56/Nop58"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047099">
        <property type="entry name" value="Nop5_N_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002687">
        <property type="entry name" value="Nop_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036070">
        <property type="entry name" value="Nop_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012976">
        <property type="entry name" value="NOSIC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894">
        <property type="entry name" value="NUCLEOLAR PROTEIN 5 NUCLEOLAR PROTEIN NOP5 NOP58"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10894:SF0">
        <property type="entry name" value="NUCLEOLAR PROTEIN 56"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01798">
        <property type="entry name" value="Nop"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21667">
        <property type="entry name" value="Nop5_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00931">
        <property type="entry name" value="NOSIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89124">
        <property type="entry name" value="Nop domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0687">Ribonucleoprotein</keyword>
    <feature type="domain" description="NOSIC" evidence="2">
        <location>
            <begin position="121"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="172"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96274.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="172" mass="20713" checksum="2E0F56B5AF13A9D0" modified="2013-06-26" version="1" fragment="single">MVYVAITPIGLFGSDKEKVEKFSDINYRKLFKEDEIVDKLYLMRTNPRKLLDEIKDWGEVKFEFFSGPNSVGNYLREHLFEIGKELKYFTDYEDFKRKMNYWNLELTKRLIKSYAQQKDKIIIQVAEAISDLDKVLNLLSERLREWYSLYFPELNSIQKHETYVKLITTLKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVA2</accession>
    <name>N6UVA2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_02969</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96289.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590740.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVA2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00141"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.582"/>
    <dbReference type="OrthoDB" id="2837at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004914">
        <property type="entry name" value="PRK06273.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF5">
        <property type="entry name" value="4FE-4S FERREDOXIN-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="44"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="86"/>
            <end position="117"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96289.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="162" mass="18681" checksum="AC7529DB8EC3A976" modified="2013-06-26" version="1">MEIKSLARIFLLGIYKNLERILFASDRVTDLELRKAILEGKIKLPNTVIKELCIGCEGCYNVCPTKAITMEKIEPVKISEYIIKDKVPKIDPKKCVYCLYCHDFCPVFSIFNEISPIHPRDVGEYVEVDLSKILKKKLEISEEQLERLSKILSLNLGKLLRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVF4</accession>
    <name>N6UVF4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Protein J422_02599</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02599</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96334.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590548.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVF4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00072"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.509"/>
    <dbReference type="OrthoDB" id="25187at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.700.20">
        <property type="entry name" value="Hypothetical protein ph0010, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.150">
        <property type="entry name" value="Hypothetical protein ph0010, domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00645">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023473">
        <property type="entry name" value="AMMECR1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036071">
        <property type="entry name" value="AMMECR1_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002733">
        <property type="entry name" value="AMMECR1_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027485">
        <property type="entry name" value="AMMECR1_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027623">
        <property type="entry name" value="AmmeMemoSam_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023472">
        <property type="entry name" value="Uncharacterised_MJ0810"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04335">
        <property type="entry name" value="AmmeMemoSam_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00296">
        <property type="entry name" value="TIGR00296 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016:SF0">
        <property type="entry name" value="AMME SYNDROME CANDIDATE GENE 1 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13016">
        <property type="entry name" value="AMMECR1 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01871">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143447">
        <property type="entry name" value="AMMECR1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51112">
        <property type="entry name" value="AMMECR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AMMECR1" evidence="2">
        <location>
            <begin position="7"/>
            <end position="197"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00645"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51112"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96334.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="201" mass="23188" checksum="359C42F648688777" modified="2013-06-26" version="1">MRLLTLEEGKFAVKFARFVIENFLKGYDIEIENYPEVFNEKRGCFCTLHTYPDRELRGCIGIPEPIMPLIEALKEAAISSATRDPRFPPVSLEEMDNIVIEISILTPPELIEVSHPREYLQKIKIGRDGLIIRFGPYSGLLLPQVPVEYGWDVEEFLANLCLKAGLPPDMWLDENVKIYKFQAQIFEEVEPRGEVIEKILY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVL1</accession>
    <name>N6UVL1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02879</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96384.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590648.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVL1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00125"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.565"/>
    <dbReference type="OrthoDB" id="2305at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.80.30.20">
        <property type="entry name" value="tm_1862 like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045784">
        <property type="entry name" value="Radical_SAM_N2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023404">
        <property type="entry name" value="rSAM_horseshoe"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731:SF1">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42731">
        <property type="entry name" value="SLL1084 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19864">
        <property type="entry name" value="Radical_SAM_N2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01082">
        <property type="entry name" value="B12-binding_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Radical SAM core" evidence="1">
        <location>
            <begin position="159"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96384.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="433" mass="50323" checksum="FE6BD8D7FD391D61" modified="2013-06-26" version="1">MKKRVAIIYPNKFKAGISCLAIHVLAKHLSKYNDLDVKVFFLENYKMIKNYDAIFITLQYEMDYFNAIRIINDLRKTNPKAVFAAGGPCVMENPFPLLNYFDVFIIGEIEKTDIMYNIIYHNFDLDGVFYKGKDKVRRVYPKKLDLEDYPIYQPTSEKGAYGKAFLLEIGRGCPRRCKFCLARVIYYPPRFRKLDDLIYLAEEGIKVNKVEKIALIAPSVGDYKYIVELCNFLNEKGVKISPSSLRADTITEELLKLLDIKTLTIAPEAGSEELRKFINKDISNEDIFNAVELAKKFGIEKVKLYYLIGLPGEKEEDIKAIASLTEKIKREIKRVEVSINPFVPKPHTEFEDFIFDIDSKKKIKLLEKMLKKIRVKVSYENFNSMLCQAVLAKGNESLGKYLDDYHKFLKVIKKDINCYLNPKEKPWKKILIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVW3</accession>
    <name>N6UVW3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Biotin--acetyl-CoA-carboxylase ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02230</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96469.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590274.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVW3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01194"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.438"/>
    <dbReference type="OrthoDB" id="46252at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004077">
        <property type="term" value="F:biotin--[biotin carboxyl-carrier protein] ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd16442">
        <property type="entry name" value="BPL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.100">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.930.10">
        <property type="entry name" value="Bira Bifunctional Protein, Domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045864">
        <property type="entry name" value="aa-tRNA-synth_II/BPL/LPL"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004408">
        <property type="entry name" value="Biotin_CoA_COase_ligase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003142">
        <property type="entry name" value="BPL_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004143">
        <property type="entry name" value="BPL_LPL_catalytic"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00121">
        <property type="entry name" value="birA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835">
        <property type="entry name" value="BIOTIN PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12835:SF5">
        <property type="entry name" value="BIOTIN--PROTEIN LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02237">
        <property type="entry name" value="BPL_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03099">
        <property type="entry name" value="BPL_LplA_LipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55681">
        <property type="entry name" value="Class II aaRS and biotin synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51733">
        <property type="entry name" value="BPL_LPL_CATALYTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="BPL/LPL catalytic" evidence="2">
        <location>
            <begin position="1"/>
            <end position="162"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51733"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96469.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="221" mass="25231" checksum="1844B25FD31DC63F" modified="2013-06-26" version="1">MKIVYLKEVDSTNDFAKKLIDNGDEAIIIAERQSKGKGRLGRIWISEEGGLYFTIILKGDPEFINFLSAVSVVETLRQYNINSGIKFPNDICVDEKKLCGVLIEIYKGYVILGIGINVNNDIKIERAVSLKSLGYDINKFELLFKFLKIFFENMKKDKEKILETYKKYSITINRPVKILLPNKIIYGKVVDIDYSGIMLDIGNKIINIPAGDVIHLRFDKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVX2</accession>
    <name>N6UVX2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphoribosyl-AMP cyclohydrolase</fullName>
            <shortName evidence="10">PRA-CH</shortName>
            <ecNumber evidence="10">3.5.4.19</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 12" type="primary">hisI</name>
        <name evidence="12" type="ORF">J422_02280</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Catalyzes the hydrolysis of the adenine ring of phosphoribosyl-AMP.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10">
            <text>1-(5-phospho-beta-D-ribosyl)-5'-AMP + H2O = 1-(5-phospho-beta-D-ribosyl)-5-[(5-phospho-beta-D-ribosylamino)methylideneamino]imidazole-4-carboxamide</text>
            <dbReference type="Rhea" id="RHEA:20049"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58435"/>
            <dbReference type="ChEBI" id="CHEBI:59457"/>
            <dbReference type="EC" id="3.5.4.19"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="10">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="2 10">Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 3/9.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 10">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the PRA-CH family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.5.4.19" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96479.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVX2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01204"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.448"/>
    <dbReference type="UniPathway" id="UPA00031">
        <property type="reaction ID" value="UER00008"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004635">
        <property type="term" value="F:phosphoribosyl-AMP cyclohydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004636">
        <property type="term" value="F:phosphoribosyl-ATP diphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000105">
        <property type="term" value="P:L-histidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.10.20.810:FF:000001">
        <property type="entry name" value="Histidine biosynthesis bifunctional protein HisIE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.20.810">
        <property type="entry name" value="Phosphoribosyl-AMP cyclohydrolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01021">
        <property type="entry name" value="HisI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026660">
        <property type="entry name" value="PRA-CH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002496">
        <property type="entry name" value="PRib_AMP_CycHydrolase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038019">
        <property type="entry name" value="PRib_AMP_CycHydrolase_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF000768">
        <property type="entry name" value="PRK00051.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42945:SF1">
        <property type="entry name" value="HISTIDINE BIOSYNTHESIS BIFUNCTIONAL PROTEIN HIS7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01502">
        <property type="entry name" value="PRA-CH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141734">
        <property type="entry name" value="HisI-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 10" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10" id="KW-0368">Histidine biosynthesis</keyword>
    <keyword evidence="7 10 12" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="Phosphoribosyl-AMP cyclohydrolase" evidence="11">
        <location>
            <begin position="29"/>
            <end position="102"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="77"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="93"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="100"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
            <note>ligand shared between dimeric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000024"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023102"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053269"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01021"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96479.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="118" mass="13915" checksum="BF6406E5CBD77AA9" modified="2013-06-26" version="1">MIEGLKFREIDGERLIVAVVCDENKNVLMVAFMNEEALKKTLETGYMHYYSTSRKKLWKKGEESGNYQKLIKVYADCDKDCLLFIVKQHGNACHEGYYSCFHYKLENGKFVLTEEYYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVX9</accession>
    <name>N6UVX9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Valine--tRNA ligase</fullName>
            <ecNumber evidence="11">6.1.1.9</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Valyl-tRNA synthetase</fullName>
            <shortName evidence="11">ValRS</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11 15" type="primary">valS</name>
        <name evidence="15" type="ORF">J422_02315</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 11">Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a 'posttransfer' editing activity that hydrolyzes mischarged Thr-tRNA(Val) in a tRNA-dependent manner.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 11">
            <text>tRNA(Val) + L-valine + ATP = L-valyl-tRNA(Val) + AMP + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:10704"/>
            <dbReference type="Rhea" id="RHEA-COMP:9672"/>
            <dbReference type="Rhea" id="RHEA-COMP:9708"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57762"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78537"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.1.1.9"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="11">ValRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated threonine is translocated from the active site to the editing site.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10 11">Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.1.1.9" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96484.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590300.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVX9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01210"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.455"/>
    <dbReference type="OrthoDB" id="23906at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002161">
        <property type="term" value="F:aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004832">
        <property type="term" value="F:valine-tRNA ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006438">
        <property type="term" value="P:valyl-tRNA aminoacylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd07962">
        <property type="entry name" value="Anticodon_Ia_Val"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00817">
        <property type="entry name" value="ValRS_core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.730.10:FF:000033">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000192">
        <property type="entry name" value="Valine--tRNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.730.10">
        <property type="entry name" value="Isoleucyl-tRNA Synthetase, Domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02005">
        <property type="entry name" value="Val_tRNA_synth_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001412">
        <property type="entry name" value="aa-tRNA-synth_I_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002300">
        <property type="entry name" value="aa-tRNA-synth_Ia"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033705">
        <property type="entry name" value="Anticodon_Ia_Val"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013155">
        <property type="entry name" value="M/V/L/I-tRNA-synth_anticd-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009080">
        <property type="entry name" value="tRNAsynth_Ia_anticodon-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009008">
        <property type="entry name" value="Val/Leu/Ile-tRNA-synth_edit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022874">
        <property type="entry name" value="Valine-tRNA_ligase_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002303">
        <property type="entry name" value="Valyl-tRNA_ligase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009687">
        <property type="entry name" value="PRK13208.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00422">
        <property type="entry name" value="valS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946:SF93">
        <property type="entry name" value="VALINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11946">
        <property type="entry name" value="VALYL-TRNA SYNTHETASES"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08264">
        <property type="entry name" value="Anticodon_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00133">
        <property type="entry name" value="tRNA-synt_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00986">
        <property type="entry name" value="TRNASYNTHVAL"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47323">
        <property type="entry name" value="Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50677">
        <property type="entry name" value="ValRS/IleRS/LeuRS editing domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00178">
        <property type="entry name" value="AA_TRNA_LIGASE_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 11 12" id="KW-0030">Aminoacyl-tRNA synthetase</keyword>
    <keyword evidence="5 11 12" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="3 11 12" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 11 12" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 11 12" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Aminoacyl-tRNA synthetase class Ia" evidence="13">
        <location>
            <begin position="15"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="domain" description="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase anticodon-binding" evidence="14">
        <location>
            <begin position="606"/>
            <end position="745"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'HIGH' region" evidence="11">
        <location>
            <begin position="43"/>
            <end position="53"/>
        </location>
    </feature>
    <feature type="short sequence motif" description="'KMSKS' region" evidence="11">
        <location>
            <begin position="525"/>
            <end position="529"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="528"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023146"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047552"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00055630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00061452"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="RuleBase" id="RU363035"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF00133"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF08264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN96484.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="871" mass="102787" checksum="CF6EE64BF0DBDED1" modified="2013-06-26" version="1">MEMPKDYNIEIEKKVQKKWENEEIYKFIENSDKPPYIIDTPPPYPTGRLHLGHALNWTYMDIIARYKRMKGYNVLFPQGWDCHGLPTEVKVEEIYNITKSDIDRKKFRELCIKLTEENIRRMREQIKSLGISIDWSREYITMKPDYIKKSQTAFVRMYKQGLIYRGEFPVNWCPRCQTAIAFAEVEYIERESYLNYIKFKGDEKDLIIATTRPELLPACVAILVHPEDERYKDIVGKEFTVPLFGQKVKVLADESVEKEFGTGAVMVCTFGDKTDVHWVNKYNLPVIKAVNERGELTEVAGKYKGLKTEEARKRIIEDLKKEGYLIKQEKIKQNVGVCWRCKTPIEIIVTKQWFVDIKKLLPKVREVVDEIKWVPEHMKIRLLNWIENMDWDWVISRQRIFATPIPIWYCRDCGNIVVAKEEDLPIDPTEKGYKCDKCGSTNLEPEKDVLDTWMDSSITPLVITKWLEDEEFFKKHYPTQLRPQGHDIIRTWAFYTILRSVALTGKKPWEEIVINGMVLGEDGHKMSKSRGNVVEPDEIIEKYGADSLRLWASNSTIGEDVQFLWKEVDYAYRFLRKFWNACRFAKMHIDNIDELKKNRKVNNIVDRWILSKLQKLIEKIDNDLSNYRFNTIVNLYKFIWHEFCDNYIEMVKYRLYEKNDLEAKYTLYYVIDKLIRLLTPFAPHFCDYISEIYNIDNLHFSFPEVNRELIDENAEKYGEIIKNTIVAIRRYKANKGLSLNAKLKEVFIYTEDEDTYKALNLGLEDIKGTMKIEDVKIIKGKPELRYKIIEIIPDKSKIGPEFKKHTKEVMEIIKNADEETLEKILSEGLKTEFGIIRREHIKDVKRALFVEGKEVEAVNIENVLALALVKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6UW35</accession>
    <name>N6UW35_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01935</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96534.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590149.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UW35"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00235"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.381"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96534.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="488" mass="53827" checksum="7AAC334780558911" modified="2013-06-26" version="1">MRGQLSLELILLTSIIILSGAILTYQFTKIPNSQLANSTEYVKEVSYALFVPNKTLKSPIVPTGSNISITIPTKNVSSEENQTINESSKEENQTFGKASRLKIIVNAESELLLTKDVLENGSWINGKLPGTIDNKSIYNANGTIHSDGTNINSGGSLKIGNLNEIESLDLIVNSDNLLAIIKDITIISANFNSINYKLNIINSHIYKSIIDNINSKGLVNIERSYVNNLNVNNAINGILNITNSNIDELNVNIINSGGCLIIKNSHIKKLNIKHNYGKVIIINSIIENRNINNENQGTTEEISSNINIDYFELRIVGNGKAILTKDIIKNASYSDSIPRLIKGKDIIKLNLLGNVYTASSSVVGNGELWLGNIKEIEKLVYEENGNAYFEINGIKINLLNSNSKAGVGNSEVLIKNCNINRSYFNRINGNSSLIIESSNIKYLEIENMISKSSLVIKNSYIEHLEISNKGKHTNIIISDSYVNGKYYS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWE4</accession>
    <name>N6UWE4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">PIN domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01303</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96634.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589924.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWE4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00029"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.255"/>
    <dbReference type="OrthoDB" id="15280at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09879">
        <property type="entry name" value="PIN_VapC_AF0591-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041120">
        <property type="entry name" value="PIN_9"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12416">
        <property type="entry name" value="RRNA-PROCESSING PROTEIN UTP23 HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18477">
        <property type="entry name" value="PIN_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="2"/>
            <end position="105"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96634.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="129" mass="14873" checksum="B5E70B9CDC862C31" modified="2013-06-26" version="1">MYLIVPDTNFLIYVFKHGINFDYEIERALNAKFDIVILSPIKKELEDLLKRDLKGKEKMAIKLALSKIGKYKVVKCEGYGDEAILNFAKNNKNVIVATNDKELKESLLNNNIPVMVVRQKKYFVVFGMV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWM6</accession>
    <name>N6UWM6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Putative (R)-citramalate synthase CimA</fullName>
            <ecNumber evidence="7">2.3.3.21</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="primary">cimA</name>
        <name evidence="10" type="ORF">J422_00846</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Catalyzes the condensation of pyruvate and acetyl-coenzyme A to form (R)-citramalate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>pyruvate + acetyl-CoA + H2O = (3R)-citramalate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19045"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30934"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="EC" id="2.3.3.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Amino-acid biosynthesis; L-isoleucine biosynthesis; 2-oxobutanoate from pyruvate: step 1/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="2 7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.3.21" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589839.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWM6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00865"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.167"/>
    <dbReference type="OrthoDB" id="6555at2157"/>
    <dbReference type="UniPathway" id="UPA00047">
        <property type="reaction ID" value="UER00066"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043714">
        <property type="term" value="F:(R)-citramalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003852">
        <property type="term" value="F:2-isopropylmalate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009097">
        <property type="term" value="P:isoleucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009098">
        <property type="term" value="P:L-leucine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07940">
        <property type="entry name" value="DRE_TIM_IPMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.260:FF:000001">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000010">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01028">
        <property type="entry name" value="CimA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013709">
        <property type="entry name" value="2-isopropylmalate_synth_dimer"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024890">
        <property type="entry name" value="Citramalate_synthase_CimA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036230">
        <property type="entry name" value="LeuA_allosteric_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002085">
        <property type="entry name" value="PRK00915.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42880:SF2">
        <property type="entry name" value="(R)-CITRAMALATE SYNTHASE CIMA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42880">
        <property type="entry name" value="HOMOCITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08502">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00917">
        <property type="entry name" value="LeuA_dimer"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110921">
        <property type="entry name" value="2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 7" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="6 7" id="KW-0100">Branched-chain amino acid biosynthesis</keyword>
    <keyword evidence="4 7" id="KW-0412">Isoleucine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="9">
        <location>
            <begin position="3"/>
            <end position="254"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022624"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023304"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="491" mass="53741" checksum="667982C3BE298DB2" modified="2013-06-26" version="1">MKVKILDTTLRDGEQTPGVSLTPYEKLEIAKKIDELGVDIIEAGSAITSKGEREAIKMIVKENLNAEICSFVRALKVDIDAALECEVDSVHLVVPSSPIHIKYKLKKSEDEVLKTATDAVEYAKDHGLIVELSAEDATRAKEEFLIELFNRGKEAGADRCCICDTVGVLTPQKAERLFKNISEKVDLPIAVHCHNDFGMATANTCSAIFAGAVEAHVTVNGIGERAGNAALEEVVTALKFLYNIDTNVKLEKLYEVSRFVAKMMKLPVPFNKAIVGDNAFAHEAGIHVDGLIKNTETYEPIKPEMVGNRRRIILGKHSGRKALKYKLDLMNIEVSEEELNKIYEKIKELGDLGKYISDTDLLAIVREVKGSGIEEKIKLDELTVVSGNKITPIASVRLHYRGENRHLIETAYGVGPVDAAINAVRKAISGVADIKLEEYSVKSIGSGTDAIVEVKVKLRRGSEVVEVVKSDADIIKASVNAVMEGINLLLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UZU8</accession>
    <name>N6UZU8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7 8">D,D-heptose 1,7-bisphosphate phosphatase</fullName>
            <ecNumber evidence="8">3.1.3.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_07167</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the HAD-like hydrolase superfamily.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the gmhB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.3.-" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000078">
        <property type="protein sequence ID" value="ENN95558.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004595163.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000078.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UZU8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01005"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1384"/>
    <dbReference type="OrthoDB" id="4991at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016791">
        <property type="term" value="F:phosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07503">
        <property type="entry name" value="HAD_HisB-N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1000">
        <property type="entry name" value="HAD superfamily/HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036412">
        <property type="entry name" value="HAD-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006439">
        <property type="entry name" value="HAD-SF_hydro_IA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006549">
        <property type="entry name" value="HAD-SF_hydro_IIIA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023214">
        <property type="entry name" value="HAD_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004446">
        <property type="entry name" value="Heptose_bisP_phosphatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006543">
        <property type="entry name" value="Histidinol-phos"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01549">
        <property type="entry name" value="HAD-SF-IA-v1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01662">
        <property type="entry name" value="HAD-SF-IIIA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01656">
        <property type="entry name" value="Histidinol-ppas"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42891">
        <property type="entry name" value="D-GLYCERO-BETA-D-MANNO-HEPTOSE-1,7-BISPHOSPHATE 7-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42891:SF1">
        <property type="entry name" value="D-GLYCERO-BETA-D-MANNO-HEPTOSE-1,7-BISPHOSPHATE 7-PHOSPHATASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13242">
        <property type="entry name" value="Hydrolase_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004682">
        <property type="entry name" value="GmhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56784">
        <property type="entry name" value="HAD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="3 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00031828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRNR" id="PIRNR004682"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95558.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="170" mass="19685" checksum="7EE84611218DCDAF" modified="2013-06-26" version="1">MNRAIFLDRDGVINKRLIGDYVKKIEEFELLPKVREALIEFKNMGYLLIVVTNQQGIAKGIMTEEDLKVVHNYMLKLLPEIDDIFYCPHLDGTCNCRKPKNGMLLKAKEKWNIDFSRSWMIGDSESDIICGKSVGCKTIRILYDNEKTEADFIAKDLYDCVKIIKNEKTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0F2</accession>
    <name>N6V0F2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Ribonuclease P protein component 3</fullName>
            <shortName evidence="6">RNase P component 3</shortName>
            <ecNumber evidence="6">3.1.26.5</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Rpp30</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rnp3</name>
        <name evidence="7" type="ORF">J422_05673</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor.</text>
            <dbReference type="EC" id="3.1.26.5"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="6">Consists of a catalytic RNA component and at least 4-5 protein subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eukaryotic/archaeal RNase P protein component 3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.5" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95803.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592784.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0F2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01268"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1106"/>
    <dbReference type="OrthoDB" id="85765at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030677">
        <property type="term" value="C:ribonuclease P complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004526">
        <property type="term" value="F:ribonuclease P activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0001682">
        <property type="term" value="P:tRNA 5'-leader removal"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00756">
        <property type="entry name" value="RNase_P_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023539">
        <property type="entry name" value="RNase_P_comp-3_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002738">
        <property type="entry name" value="RNase_P_p30"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF046108">
        <property type="entry name" value="RNaseP3Mthcoc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01876">
        <property type="entry name" value="RNase_P_p30"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="4 6" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="5 6" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3 6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 6" id="KW-0819">tRNA processing</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00756"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95803.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="227" mass="27221" checksum="1C569F4486B00AD7" modified="2013-06-26" version="1">MRIDLNRIDSEEGIKLLKELKWQGFVYYQYNNFDKEKFLEVKEIAERYNLKVYSGIKLRDNNIKSLKNSIKKYRRKCHIILIEGGDIKINRFSCEQHNVDILSTPELNRKDSGMDHVLMRLASEHRVAIEINFRELLLKDGYERARLMLFFRRNLMLAKKYKTPVVVSTDATNIYEIKSPYDIRAFLNTLVEPEFAKKIMETLYKICEYRDYLMRDNVIRYGLEIIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0G9</accession>
    <name>N6V0G9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05763</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95818.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592812.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0G9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01253"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1123"/>
    <dbReference type="OrthoDB" id="64436at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019211">
        <property type="entry name" value="EhaL"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09877">
        <property type="entry name" value="EhaL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="37"/>
            <end position="54"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="74"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95818.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="101" mass="11436" checksum="1E1ED20F743405B0" modified="2013-06-26" version="1">MNLFEINIAIVCFIVGNFLGLNYSYNKYPFPYVENKIDMLALILSILGGILLNTPLYDLGILLLSFPFGMRPGYGRIEFILGLSLAIILYLVKKWMLIGNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6V153</accession>
    <name>N6V153_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">CRISPR-associated protein Cas6</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_04735</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated protein Cas6/Cse3/CasE family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96018.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V153"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00966"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.926"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.1900">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049435">
        <property type="entry name" value="Cas_Cas6_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010156">
        <property type="entry name" value="CRISPR-assoc_prot_Cas6"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01877">
        <property type="entry name" value="cas_cas6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36984">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36984:SF1">
        <property type="entry name" value="CRISPR-ASSOCIATED ENDORIBONUCLEASE CAS6 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01881">
        <property type="entry name" value="Cas_Cas6_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRISPR associated protein Cas6 C-terminal" evidence="4">
        <location>
            <begin position="57"/>
            <end position="167"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005937"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96018.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="181" mass="21361" checksum="ECC02C88A903611D" modified="2013-06-26" version="1">MGIKTLDGRIFLHIASPNNEFLKTFIKGILECESFKVDNIELVPITLTFEDIPKQFNILKTISPIYLRTTIEENGKKKIYDLLPNHSKFYENLKKNLKMKYEAFYNKKCNFDFDFEILKFKTKRMLVKNTYCRCTEMVFRVWGDYEIIKFGYECGFGEWNSMGFGMVIEGKDENNSGNNRS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>N6V174</accession>
    <name>N6V174_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04590</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000029">
        <property type="protein sequence ID" value="ENN96033.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591913.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V174"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.899"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96033.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="72" mass="8762" checksum="3D728B4AB3FE1145" modified="2013-06-26" version="1">MGYWFGVFDKRLSWRKKTPVKIYGKWYTPKGEKVRDSDAYFKTIERNGRYWEGNIGWSKKNKKILRDHGVFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1L5</accession>
    <name>N6V1L5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Sirohydrochlorin cobaltochelatase</fullName>
            <ecNumber evidence="6">4.99.1.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">CbiXS</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Sirohydrochlorin nickelchelatase</fullName>
            <ecNumber evidence="6">4.99.1.11</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">cbiX</name>
        <name evidence="6" type="synonym">cfbA</name>
        <name evidence="7" type="ORF">J422_03933</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the insertion of Co(2+) into sirohydrochlorin as part of the anaerobic pathway to cobalamin biosynthesis. Involved in the biosynthesis of the unique nickel-containing tetrapyrrole coenzyme F430, the prosthetic group of methyl-coenzyme M reductase (MCR), which plays a key role in methanogenesis and anaerobic methane oxidation. Catalyzes the insertion of Ni(2+) into sirohydrochlorin to yield Ni-sirohydrochlorin.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Co-sirohydrochlorin + 2 H(+) = sirohydrochlorin + Co(2+)</text>
            <dbReference type="Rhea" id="RHEA:15893"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:60049"/>
            <dbReference type="EC" id="4.99.1.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6">
            <text>Ni-sirohydrochlorin + 2 H(+) = sirohydrochlorin + Ni(2+)</text>
            <dbReference type="Rhea" id="RHEA:52796"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <dbReference type="ChEBI" id="CHEBI:58351"/>
            <dbReference type="ChEBI" id="CHEBI:136841"/>
            <dbReference type="EC" id="4.99.1.11"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Cofactor biosynthesis; adenosylcobalamin biosynthesis; cob(II)yrinate a,c-diamide from sirohydrochlorin (anaerobic route): step 1/10.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homotetramer; dimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the CbiX family. CbiXS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.99.1.3" evidence="6"/>
    <dbReference type="EC" id="4.99.1.11" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96173.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1L5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00416"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.771"/>
    <dbReference type="UniPathway" id="UPA00148">
        <property type="reaction ID" value="UER00223"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0050897">
        <property type="term" value="F:cobalt ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016852">
        <property type="term" value="F:sirohydrochlorin cobaltochelatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019251">
        <property type="term" value="P:anaerobic cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015948">
        <property type="term" value="P:methanogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd03416">
        <property type="entry name" value="CbiX_SirB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1400">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00785">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002762">
        <property type="entry name" value="CbiX-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023652">
        <property type="entry name" value="SiroHydchlorin_Cochelatase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050963">
        <property type="entry name" value="Sirohydro_Cobaltochel/CbiX"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF033198">
        <property type="entry name" value="F430_CfbA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002090">
        <property type="entry name" value="PRK00923.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33542">
        <property type="entry name" value="SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33542:SF3">
        <property type="entry name" value="SIROHYDROCHLORIN FERROCHELATASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01903">
        <property type="entry name" value="CbiX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53800">
        <property type="entry name" value="Chelatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="5 6" id="KW-0170">Cobalt</keyword>
    <keyword evidence="4 6 7" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="2 6" id="KW-0533">Nickel</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="9"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="9"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="70"/>
            <end position="75"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="75"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022596"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00785"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96173.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="123" mass="14223" checksum="4B65AD4BA8DF0371" modified="2013-06-26" version="1">MEALILVGHGSRYPYSKELMEKIAEKIRERNLFPIVEIGMMEFNEPTIPQALKKVIEMGAKRIIVVPVFLAHGTHTLKDIPHLLGLKELDENDSHHHHHDHEHVEIPEDVEIIYREPIGLMID</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6V236</accession>
    <name>N6V236_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Lipoprotein B LppB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02669</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96348.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590575.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V236"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00086"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.523"/>
    <dbReference type="OrthoDB" id="10182at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051311">
        <property type="entry name" value="DedA_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032816">
        <property type="entry name" value="VTT_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709">
        <property type="entry name" value="ALKALINE PHOSPHATASE LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42709:SF11">
        <property type="entry name" value="DEDA FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09335">
        <property type="entry name" value="VTT_dom"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0449">Lipoprotein</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="52"/>
            <end position="73"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="135"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="domain" description="VTT" evidence="2">
        <location>
            <begin position="33"/>
            <end position="149"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF09335"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96348.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="176" mass="19934" checksum="F27DC04EC03BA5E3" modified="2013-06-26" version="1">MDLYIVAKEIILNYGYFGIFFLSMIEAIFFPIPPDVFLLSSTYFGLDPLLSTVMATLGTVVGGTIGYFLGYKFGHPIFLKLFNEKYLIKGEEFFNKYGVFGIVIAGFTPIPYKVIAWLSGIFEMNLCLFVIATFIGRFPRFIMVAYFGDLIKNLKIGAILAFFIFALCLIIIKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2E8</accession>
    <name>N6V2E8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">AAA ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_02395</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="4">Belongs to the AAA ATPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96438.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590360.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2E8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00360"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.471"/>
    <dbReference type="OrthoDB" id="77269at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004176">
        <property type="term" value="F:ATP-dependent peptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd19481">
        <property type="entry name" value="RecA-like_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:001025">
        <property type="entry name" value="ATPase family, AAA domain-containing 2B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003960">
        <property type="entry name" value="ATPase_AAA_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057405">
        <property type="entry name" value="PRS2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057408">
        <property type="entry name" value="PRS2_C_AAA_lid"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076:SF97">
        <property type="entry name" value="ATP-DEPENDENT ZINC METALLOPROTEASE YME1L1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23076">
        <property type="entry name" value="METALLOPROTEASE M41 FTSH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23902">
        <property type="entry name" value="AAA_lid_PRS2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23900">
        <property type="entry name" value="PRS2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00674">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="5">
        <location>
            <begin position="153"/>
            <end position="288"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96438.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="371" mass="42276" checksum="A9FE2DCB2D220707" modified="2013-06-26" version="1">MSKISINPLKIKSFSKIKTYEDPIPSLKLVVLEPVGFPIKVSNENVRVTTDDPVLFNIYARDQWIGEIVKEGDYLFDNSILPDYAFKVVSTYPKEGGMITSETVFKLQTPKKIIRTHFKKARFDEIIGQEEAKKKCKIIMKYLENPKLFGEWAPKNILFYGPPGTGKTLMARALATETNSSFLLVKAPELIGEHVGDAAKMIRELYQKASESAPCIIFIDELDAIGLSREYQSLRGDVSEVVNALLTELDGIKEHEGVVTIGATNNPSMLDPALRSRFEEEIEFKLPNDEERLKIMELYAKKMPIPVKANLKEFVDKTKGFSGRDIKEKFLKPALHRAILEDRNYVSKEDLEWALKKILEKRKEAPHHLYI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2F3</accession>
    <name>N6V2F3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">NADH dehydrogenase (Ubiquinone), 30 kDa subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_02100</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96443.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590220.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2F3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01169"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.413"/>
    <dbReference type="OrthoDB" id="43567at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008901">
        <property type="term" value="F:ferredoxin hydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016151">
        <property type="term" value="F:nickel cation binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016651">
        <property type="term" value="F:oxidoreductase activity, acting on NAD(P)H"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0048038">
        <property type="term" value="F:quinone binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.645.10">
        <property type="entry name" value="Cytochrome-c3 Hydrogenase, chain B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052197">
        <property type="entry name" value="ComplexI_49kDa-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001135">
        <property type="entry name" value="NADH_Q_OxRdtase_suD"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001501">
        <property type="entry name" value="Ni-dep_hyd_lsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018194">
        <property type="entry name" value="Ni-dep_hyd_lsu_Ni_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029014">
        <property type="entry name" value="NiFe-Hase_large"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43485:SF1">
        <property type="entry name" value="FORMATE HYDROGENLYASE SUBUNIT 5-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43485">
        <property type="entry name" value="HYDROGENASE-4 COMPONENT G"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00346">
        <property type="entry name" value="Complex1_49kDa"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00374">
        <property type="entry name" value="NiFeSe_Hases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56762">
        <property type="entry name" value="HydB/Nqo4-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00507">
        <property type="entry name" value="NI_HGENASE_L_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="2" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0533">Nickel</keyword>
    <keyword evidence="1" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0830">Ubiquinone</keyword>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="3">
        <location>
            <begin position="115"/>
            <end position="283"/>
        </location>
    </feature>
    <feature type="domain" description="NADH-quinone oxidoreductase subunit D" evidence="3">
        <location>
            <begin position="297"/>
            <end position="361"/>
        </location>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="42"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="322"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="355"/>
        </location>
        <ligand>
            <name>Ni(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="2">
        <location>
            <position position="358"/>
        </location>
        <ligand>
            <name>Fe cation</name>
            <dbReference type="ChEBI" id="CHEBI:24875"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRSR" id="PIRSR601501-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00346"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96443.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="370" mass="42517" checksum="2FA61E1AA8AC9B36" modified="2013-06-26" version="1">MEIAIGPIHSAMLEPHRLRLVVEDEIIKDAEITIGVNYRGIELIMEGLPPEKISILSEKICGICSHIHVWTNVMVVEKGCDIEVPERAEYIRIIVEELERLHSHTLLFGHIYEVLGFETMAYRAFMVREPILKILQEITGGRVQYSCPIIGGVRPRCNIQENKIPSILERLEKFEEDLKKLLKRTFNDPMILARIKEVGYLDKETAKKFHATGPTARGSGIRSDMRKLLKVSLYDRFEFDEVLFDEGDVYARLAVRFYECFESLKIIRQALKDLPNLDKKIYNPNYELKPFDPIDVYNEAQRGQVYYSYGLDENLRVKHVKVRTPTATNLACMEAILPGHNVSDAELIIVSCDPCFTCTDRMIVIKESSP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2F8</accession>
    <name>N6V2F8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Thiamine-binding protein domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02125</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0045 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96448.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590225.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2F8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01174"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.418"/>
    <dbReference type="OrthoDB" id="10763at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.930">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029756">
        <property type="entry name" value="MTH1187/YkoF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002767">
        <property type="entry name" value="Thiamine_BP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051614">
        <property type="entry name" value="UPF0045_domain"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00106">
        <property type="entry name" value="MTH1187 family thiamine-binding protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33777:SF1">
        <property type="entry name" value="UPF0045 PROTEIN ECM15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01910">
        <property type="entry name" value="Thiamine_BP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89957">
        <property type="entry name" value="MTH1187/YkoF-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Thiamine-binding protein" evidence="2">
        <location>
            <begin position="6"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010272"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01910"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96448.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="97" mass="11154" checksum="2B2AB77698E9FBA4" modified="2013-06-26" version="1">MRKVVAEVKIIPMGEVSVSKYIKRAIEIFKKYNLKVSPSAMGTVLEGDIDEILKAYKEAHTEILNYVDRVVSFLEIDERRDKENTIERKLKAIQVGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2K7</accession>
    <name>N6V2K7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF357 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01700</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96493.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590098.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2K7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00191"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.334"/>
    <dbReference type="OrthoDB" id="148073at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1270.90">
        <property type="entry name" value="AF1782-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036809">
        <property type="entry name" value="AF1782-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023140">
        <property type="entry name" value="DUF357"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04010">
        <property type="entry name" value="DUF357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158372">
        <property type="entry name" value="AF1782-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF357" evidence="1">
        <location>
            <begin position="11"/>
            <end position="84"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96493.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="97" mass="11292" checksum="6C6024E66889F0F8" modified="2013-06-26" version="1">MREITDEKLEKYFKMTEEAIKIIKNGMPPKRSLLYDVAKDFLLMIESYYKDAKSFKEKGDYVNAFASLNYAYGWIDAGVRLGLFNVGDDDKRFTLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2L0</accession>
    <name>N6V2L0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Ferredoxin</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_01725</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Ferredoxins are iron-sulfur proteins that transfer electrons in a wide variety of metabolic reactions.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 8">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96498.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590103.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2L0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00196"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.339"/>
    <dbReference type="OrthoDB" id="2837at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009055">
        <property type="term" value="F:electron transfer activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd04410">
        <property type="entry name" value="DMSOR_beta-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000813">
        <property type="entry name" value="7Fe_ferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050294">
        <property type="entry name" value="RnfB_subfamily"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42859:SF10">
        <property type="entry name" value="DIMETHYLSULFOXIDE REDUCTASE CHAIN B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42859">
        <property type="entry name" value="OXIDOREDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00354">
        <property type="entry name" value="7FE8SFRDOXIN"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3 8" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5 8" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6 8" id="KW-0408">Iron</keyword>
    <keyword evidence="7 8" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 8" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="9">
        <location>
            <begin position="66"/>
            <end position="95"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU365098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96498.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="161" mass="17710" checksum="2CD03DA13C92D880" modified="2013-06-26" version="1">MKKVMMTNFLCDNCGDCVRACMEVNKVSRIYIIEKDKKFVPIVCQHCASAPCKEVCPVNAIYHENSVVKLDLDKCIGCGLCALACPFGAIVIDDKAYKCVLCKETACVKACSKGCLEIVEVNDIINLRKEKASELFVKIKVNGSKKGEIIDKIVANAKIQI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2Z5</accession>
    <name>N6V2Z5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Transcriptional regulator, MarR family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_01368</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96608.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2Z5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00015"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.268"/>
    <dbReference type="OrthoDB" id="27885at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR055767">
        <property type="entry name" value="DUF7343"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001845">
        <property type="entry name" value="HTH_ArsR_DNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF24034">
        <property type="entry name" value="DUF7343"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50987">
        <property type="entry name" value="HTH_ARSR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="197"/>
            <end position="216"/>
        </location>
    </feature>
    <feature type="domain" description="HTH arsR-type" evidence="3">
        <location>
            <begin position="323"/>
            <end position="405"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="1">
        <location>
            <begin position="232"/>
            <end position="329"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96608.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="405" mass="46983" checksum="5E7EC46FC85DEDEB" modified="2013-06-26" version="1">MLILLLFLPNILAYQIENENIVCFIHNNNLLENITLEFKNFENNKSTFYITLPQNVKILNISSNYPIDGYSAVFQDGTSNIAVAFEKPLPINKTVSLTLTCLVKDCIWKGYDYYQFITNFPIIGKNVKIKLYLPPGAVIVYPNLITPEGYEITGDGKHQIIVWNLKPKSEFIMTTSLRFAYAPYTPITNKEKKDNNIIIFLILLSGSIAFVAYERFKRIKINKEKNQIYDNYLEIKGQLAEKNKQISDLEDKCRFLEEEVGKLKNIIEEKDKVICEMSKKVEEYLKEIKLLGKEKEDLINNITELKAKIEKLKDENEKLKNIIDKYKDSEKGILLNFLTDDEKKIIKLIKEHKYITQKDIVSLTRMSKSKVSRIVSELEERGIVKKEKVGRINMLTLTDEADNLI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3K5</accession>
    <name>N6V3K5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Coenzyme F420-reducing hydrogenase subunit delta</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_00285</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the peptidase A31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96838.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589744.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3K5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01106"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.57"/>
    <dbReference type="OrthoDB" id="85598at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004190">
        <property type="term" value="F:aspartic-type endopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008047">
        <property type="term" value="F:enzyme activator activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016485">
        <property type="term" value="P:protein processing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06064">
        <property type="entry name" value="H2MP_F420-Reduc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1450:FF:000005">
        <property type="entry name" value="Hydrogenase maturation protease HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1450">
        <property type="entry name" value="HybD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004411">
        <property type="entry name" value="Pept_A31_F420-red_hyd_d"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023430">
        <property type="entry name" value="Pept_HybD-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000671">
        <property type="entry name" value="Peptidase_A31"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00130">
        <property type="entry name" value="frhD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00072">
        <property type="entry name" value="hydrog_prot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302">
        <property type="entry name" value="HYDROGENASE 1 MATURATION PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30302:SF1">
        <property type="entry name" value="HYDROGENASE 2 MATURATION PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01750">
        <property type="entry name" value="HycI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00446">
        <property type="entry name" value="HYDRGNUPTAKE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53163">
        <property type="entry name" value="HybD-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0064">Aspartyl protease</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0645">Protease</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022750"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96838.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="149" mass="16374" checksum="77B6F3A17A6C1A65" modified="2013-06-26" version="1">MILIVGCGNVLFGDDGFGYEVIKRLEEIKLPENVKLIDAGASGAYYLMDIVDENVEKIIIVDAIDYGLKPGEIKILSVDELPNIRKYTFDAHAVPLAPFLKDLAEKGIEVKVIGCQVKNLPIPEINPGLSEEVKKAVDKAVKIILEEIL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3L0</accession>
    <name>N6V3L0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Probable inosine/xanthosine triphosphatase</fullName>
            <shortName evidence="10">ITPase/XTPase</shortName>
            <ecNumber evidence="10">3.6.1.73</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Non-canonical purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Non-standard purine NTP phosphatase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="10">Nucleoside-triphosphate phosphatase</fullName>
            <shortName evidence="10">NTPase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_00310</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Phosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP to their respective diphosphate derivatives. Probably excludes non-canonical purines from DNA/RNA precursor pool, thus preventing their incorporation into DNA/RNA and avoiding chromosomal lesions.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>ITP + H2O = IDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28330"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58280"/>
            <dbReference type="ChEBI" id="CHEBI:61402"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>XTP + H2O = XDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:28406"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59884"/>
            <dbReference type="ChEBI" id="CHEBI:61314"/>
            <dbReference type="EC" id="3.6.1.73"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <text evidence="10">Binds 1 divalent metal cation per subunit; can use either Mg(2+) or Mn(2+).</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="10">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the YjjX NTPase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.73" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96843.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589749.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3L0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01101"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.62"/>
    <dbReference type="OrthoDB" id="52857at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0103023">
        <property type="term" value="F:ITPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009117">
        <property type="term" value="P:nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006772">
        <property type="term" value="P:thiamine metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="3.90.950.10:FF:000002">
        <property type="entry name" value="Inosine/xanthosine triphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.950.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00648">
        <property type="entry name" value="Non_canon_purine_NTPase_YjjX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029001">
        <property type="entry name" value="ITPase-like_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002786">
        <property type="entry name" value="Non_canon_purine_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026533">
        <property type="entry name" value="NTPase/PRRC1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050299">
        <property type="entry name" value="YjjX_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00258">
        <property type="entry name" value="inosine/xanthosine triphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34699">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34699:SF2">
        <property type="entry name" value="NON-CANONICAL PURINE NTP PHOSPHATASE_PRRC1 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01931">
        <property type="entry name" value="NTPase_I-T"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52972">
        <property type="entry name" value="ITPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 10" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="5 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7 10" id="KW-0464">Manganese</keyword>
    <keyword evidence="2 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10" id="KW-0546">Nucleotide metabolism</keyword>
    <keyword evidence="3 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Non-canonical purine NTP phosphatase/PRRC1" evidence="11">
        <location>
            <begin position="47"/>
            <end position="207"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="76"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="105"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023080"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00048174"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00648"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF01931"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96843.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="214" mass="23922" checksum="8B0CF4393DEF2E2F" modified="2013-06-26" version="1">MEKACEICGKKAEIFLYGMHLCKDIKCWEEAKKISLARHKMRVVALGSKNPIKVKATKEAFERVIGYSEIIPVDVDSKVSPHPIGLEETYKGALNRAKEAFKKIQCVYSVGIEAGMIELDGHYLDIHVAVVYDGIRETVGLSQGFEYPKIVAEHIMKGIEGKIIAEKISGIKGIGESVGMIGYLTDNHIKREDLCRDAVIMALIPRMKRNVELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP59</accession>
    <name>N6VP59_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Polysaccharide biosynthesis protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_06768</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000059">
        <property type="protein sequence ID" value="ENN95640.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594062.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000059.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP59"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00982"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1315"/>
    <dbReference type="OrthoDB" id="19148at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="CDD" id="cd13128">
        <property type="entry name" value="MATE_Wzx_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050833">
        <property type="entry name" value="Poly_Biosynth_Transport"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002797">
        <property type="entry name" value="Polysacc_synth"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250:SF27">
        <property type="entry name" value="POLYSACCHARIDE BIOSYNTHESIS PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30250">
        <property type="entry name" value="PST FAMILY PREDICTED COLANIC ACID TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01943">
        <property type="entry name" value="Polysacc_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="23"/>
            <end position="41"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="62"/>
            <end position="82"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="88"/>
            <end position="107"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="128"/>
            <end position="149"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="169"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="211"/>
            <end position="229"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="249"/>
            <end position="269"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="281"/>
            <end position="297"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="303"/>
            <end position="325"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="337"/>
            <end position="355"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="361"/>
            <end position="386"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="7">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95640.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="401" mass="45406" checksum="496018CFA6EAB41B" modified="2013-06-26" version="1" fragment="single">LLSPLIAEYYINNQGQFTGHLDLVINVLIILSVGFWFQSIMDVISSSIQGFQNQKYRGTISFVRISLILILSAIFIYFFGFHNVFVPTYAYTLTPVLMILIYAYIFFKKIFPEFFEVKFLLSKKLIKDLFSYGIYIMIGSAGSLVLGYIDGICLTYFIGLNAVADYRNVAVPTVSVLSYFASSVTTVLFPLSSELWVKGYKKALGYGVEKICLYSFILVLPMAILMTYFPKVIINLFFNPQYLSAADAIRILSLGMIFMTLNNIGFTVLNGIGKPHLSTKILYVGAIFNLIFNILLIPKFGIVGASLTTVFGYLLMWILQVKYLNKFLNYSFLIKKWILVTLIGIFSLIPLIIITKIINNIILQLILGGIVYFGIYLIGIFGLRIIDVKEIYDMLNKIRGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP70</accession>
    <name>N6VP70_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Serine dehydrogenase proteinase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06608</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000056">
        <property type="protein sequence ID" value="ENN95660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593799.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP70"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00361"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1286"/>
    <dbReference type="OrthoDB" id="146310at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.226.10">
        <property type="entry name" value="2-enoyl-CoA Hydratase, Chain A, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029045">
        <property type="entry name" value="ClpP/crotonase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002825">
        <property type="entry name" value="Pept_S49_ser-pept_pro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35984">
        <property type="entry name" value="PERIPLASMIC SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35984:SF1">
        <property type="entry name" value="PERIPLASMIC SERINE PROTEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01972">
        <property type="entry name" value="SDH_protease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52096">
        <property type="entry name" value="ClpP/crotonase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="171" mass="19269" checksum="F4A74D538529D5CB" modified="2013-06-26" version="1" fragment="single">ALKEHPAETRVIIPHYAMSGGTLIALAADKIIMDKNAVLGPVDPQLGQYPAVSILEAYCMKGEKLNDETLILVDIARKAIKQMEEFVYNLLKDKYGEEKAKKIAKILSNGKWTHDYPLTVQKLKEIGIEVDTNVPKKVYELLDLYPQPRGAKPSVYYIPIPYKKEGNNEKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPA7</accession>
    <name>N6VPA7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">CBS domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_06476</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95710.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593554.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPA7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01280"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1261"/>
    <dbReference type="OrthoDB" id="295172at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.580.10">
        <property type="entry name" value="CBS-domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051257">
        <property type="entry name" value="Diverse_CBS-Domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002708">
        <property type="entry name" value="HcyBio"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016426">
        <property type="entry name" value="MA1821-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080:SF2">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43080">
        <property type="entry name" value="CBS DOMAIN-CONTAINING PROTEIN CBSX3, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01837">
        <property type="entry name" value="HcyBio"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004698">
        <property type="entry name" value="UCP004698_CBS_MJ0100"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="392"/>
            <end position="451"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="4">
        <location>
            <begin position="453"/>
            <end position="507"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95710.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="507" mass="56043" checksum="F559FEC4592E9D17" modified="2013-06-26" version="1">MKSIAEINEKIKKGEAVVVTAEEMIKIVEEEGAKKAAEYVDVVTTGTFGAMCSSGVFINFGHSDPPIKMLKIYLNNVECYGGLAAVDTYLGAAQPNEDPDIDIEYGGAHVIEDLIRGKEVELYAEGYTTDCYPRKEINVKITLDDVNQAIMVNPRNCYQTYTAATNSREEKIYTYMGILLPEYNNVHYSGAGQLNPLQNDYDPKTGEFNTIGIGTKIFLGGGVGYVIGEGTQHNPPFGTLMVKGDLKQMNAKYVRAATMPRYGSTLYIGIGIPIPVLNEKIAKRCAIRDEDIEVPIYDYGVPRRDRPLVGKTNYKELRSGKIVLEVEIDNKRVEKAVKTGPVSSYKMAREVAKELKNWILNGEFLLTERVAPLGKPEFKPMKSPITLVKDIMSSPPIVANPNITINEAANVLIKYDINHLPIVDSEGRLIGIVTSWDIAKALALNKKKIDEIMTKNVVVAYKDEPIDVVARRMSLNKISGMPVIDENKRVIGVITSEDISGLLGEKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPC0</accession>
    <name>N6VPC0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane-bound metal-dependent hydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06209</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95725.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593338.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPC0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00296"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1209"/>
    <dbReference type="OrthoDB" id="118042at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007404">
        <property type="entry name" value="YdjM-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04307">
        <property type="entry name" value="YdjM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="48"/>
            <end position="65"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="72"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="103"/>
            <end position="131"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="138"/>
            <end position="156"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="168"/>
            <end position="194"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="206"/>
            <end position="231"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95725.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="232" mass="26266" checksum="FB29A1B0CD300B9F" modified="2013-06-26" version="1">MDWKGHTILGLVFGLPFISSPEQIFLALAGALYPDLDHDVKEDIVKRGIYISLGLVLINILIYYFKKEYFNLDLFILSVSVLLIYLIPYFANHRGITHTFWTLILVSVILGFLALKLTIFSPVLAGIIVLLMVVNEKLLGKILIIAIFGWIVLDILKPHSLVSGNLSYILPVAVGYLSHIVGDSITPAGVKAFYPISKYKLRKKEGYILILVWFLMVLYIWKDIILSLLIFK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPD9</accession>
    <name>N6VPD9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Polyprenyl synthetase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06075</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95745.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593231.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPD9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00534"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1183"/>
    <dbReference type="OrthoDB" id="120943at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002829">
        <property type="entry name" value="DUF116"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43801">
        <property type="entry name" value="NUCLEOTIDE-BINDING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43801:SF1">
        <property type="entry name" value="POLYPRENYL SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01976">
        <property type="entry name" value="DUF116"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006594">
        <property type="entry name" value="UCP006594"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="31"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="43"/>
            <end position="62"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95745.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="206" mass="23431" checksum="26B9FF69770FCDE4" modified="2013-06-26" version="1">MGILEIIGLITVLIITLIIIATLTLLIVGYVLIKKNKILFPKLALFLLDNFYSILLRLFLFIGSEETFYRIGIEFYNKYYEDKFKSTNKKILILPHCLRDAKCPAKLSPNGIECVMCGKCVIGEILKRAKSLGYEVYIVPGSTFLKRILKNDSGVFGVACYKDLFYGMNYLSRKSIPMQGQPLLKDGCLNTIVDVDELLNRLENLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPN1</accession>
    <name>N6VPN1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">AIG2 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05519</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000042">
        <property type="protein sequence ID" value="ENN95855.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592596.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000042.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPN1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00744"/>
    <dbReference type="OrthoDB" id="100169at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd06661">
        <property type="entry name" value="GGCT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.490.10">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009288">
        <property type="entry name" value="AIG2-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013024">
        <property type="entry name" value="GGCT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036568">
        <property type="entry name" value="GGCT-like_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06094">
        <property type="entry name" value="GGACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF110857">
        <property type="entry name" value="Gamma-glutamyl cyclotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Gamma-glutamylcyclotransferase AIG2-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="2">
        <location>
            <position position="46"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06094"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95855.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="46" mass="5523" checksum="7CF56F5C5DF23E8E" modified="2013-06-26" version="1" fragment="single">MQYIFVYGTLRRGFWNNKLLKNSKFIGKGRTKEKYAMYADIIPYVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPQ1</accession>
    <name>N6VPQ1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">CRM domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_05504</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000041">
        <property type="protein sequence ID" value="ENN95875.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592565.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000041.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPQ1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01409"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1075"/>
    <dbReference type="OrthoDB" id="30785at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.110.60">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001890">
        <property type="entry name" value="RNA-binding_CRM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051925">
        <property type="entry name" value="RNA-binding_domain"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017924">
        <property type="entry name" value="RNA-binding_YhbY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035920">
        <property type="entry name" value="YhbY-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00253">
        <property type="entry name" value="RNA_bind_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40065">
        <property type="entry name" value="RNA-BINDING PROTEIN YHBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40065:SF3">
        <property type="entry name" value="RNA-BINDING PROTEIN YHBY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01985">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01103">
        <property type="entry name" value="CRS1_YhbY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75471">
        <property type="entry name" value="YhbY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51295">
        <property type="entry name" value="CRM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 2" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRM" evidence="3">
        <location>
            <begin position="3"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00626"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS51295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95875.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="127" mass="14822" checksum="D20900F7D08CFD6C" modified="2013-06-26" version="1">MEKRLTGKMKRMLRAKAHHLKPVVWVGKEGSDKVIKEVERQLKERGLIKIKVRKSALLKEDKYEIAEKLAKACDAEVVSVIGHIITLFKPREGWGRYLSKKPKKVKKDEKIVKLFEEFKRSIKKKGE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPQ6</accession>
    <name>N6VPQ6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methanogenesis marker protein 7</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05409</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000039">
        <property type="protein sequence ID" value="ENN95880.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592472.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPQ6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00843"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1056"/>
    <dbReference type="OrthoDB" id="52512at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026327">
        <property type="entry name" value="Me_CoM_Rdtase_prot-C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011312">
        <property type="entry name" value="Menthan_mark_7"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03274">
        <property type="entry name" value="methan_mark_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04609">
        <property type="entry name" value="MCR_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019164">
        <property type="entry name" value="UCP019164"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95880.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="306" mass="35232" checksum="AD0876C4CD004B5B" modified="2013-06-26" version="1">MYEIVRYEGGVYKNNVFKEWIEDIGGFILQEHVMQLDVYMTLAIPYNELERLKEEAKKYKGKIIETPLAGTEIAVVAPSLSRHHLPHIACDVAEYLRRFGAKSNMIGLARGVGKDIAQIREIEKDLIEEHDLAVYVMGNFEACIKDKVHLFDVEIPVVVTGGPEKIDIPYPYVGNLGRRSHRLRRGEEIRALRKMVEVITELINNRRKELSYDPPVVPPVVVKDEIEKQVDEVARVLSPLPIVTQLDGLRVKLDYDKYVNKIKEVKIGEYRLDEIANIKRSVMKNYILIKLKPKSEVEFEREMHGA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPS6</accession>
    <name>N6VPS6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Pyruvate kinase C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05329</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95900.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592379.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPS6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01321"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1040"/>
    <dbReference type="OrthoDB" id="64834at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1380.20">
        <property type="entry name" value="Pyruvate kinase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015074">
        <property type="entry name" value="DUF1867"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015795">
        <property type="entry name" value="Pyrv_Knase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036918">
        <property type="entry name" value="Pyrv_Knase_C_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02887">
        <property type="entry name" value="PK_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016138">
        <property type="entry name" value="UCP016138"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52935">
        <property type="entry name" value="PK C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Pyruvate kinase C-terminal" evidence="1">
        <location>
            <begin position="14"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF02887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95900.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="190" mass="20791" checksum="567756CD1B39C977" modified="2013-06-26" version="1">MKLFEKPGKENTDETLKISVERAKRGDIKSIVVASSRGDTAKKLLDLLEKEGLKLNVVVVTYHQGFHGEDIISMPKDVEEELIKRGAKVFRGTHALSGVERGISNKLGGYGPVQVIAETLRTFGQGVKVCYEITVMACDAGLIKSKEEVIAIGGTSRGADTAMVIKPANMNNFFDIEAREILCMPRVKKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPU1</accession>
    <name>N6VPU1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ABC transporter ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_05209</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000034">
        <property type="protein sequence ID" value="ENN95920.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592262.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000034.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPU1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00307"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1017"/>
    <dbReference type="OrthoDB" id="18388at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00267">
        <property type="entry name" value="ABC_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046834">
        <property type="entry name" value="ABC_ATPase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019195">
        <property type="entry name" value="ABC_ATPase_put"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046833">
        <property type="entry name" value="ABC_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38149">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38149:SF1">
        <property type="entry name" value="ATPASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09818">
        <property type="entry name" value="ABC_ATPase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20446">
        <property type="entry name" value="ABC_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATPase of the ABC class N-terminal" evidence="2">
        <location>
            <begin position="14"/>
            <end position="161"/>
        </location>
    </feature>
    <feature type="domain" description="ATPase of the ABC class C-terminal" evidence="1">
        <location>
            <begin position="166"/>
            <end position="432"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09818"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20446"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95920.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="441" mass="50648" checksum="9A7D1D79BC7D5C7C" modified="2013-06-26" version="1">MIDSILSHITCPFLDNRLITKANKNKIYYAVKQPDGNIKVVLPFVFENEKFLKLSDYKDGIEGATQRVIEEIKSEIINKKRFLPLAGYFGKRYRSLYEPLTVVNCTLNIGSDLWRADDYNYIDGNKIYLLLRMVFKERDEKVIAEKIDEIGYGLERLIKNINLNILIDEAKNIIDQKFLREKLKELNLVSFIANDSKPARKYTEVRKHYRIAGPKEINIPFVCPKELKPIEIELKYRVVEGLGIKRKEVFVITGRNAQGKTTMLQAIESGQDDHIIGDGREFIITVRNLIKATTGSMEMNGEDISLFFQKLPKGIRGSPKAVYGTASGSMYMAYQIQKAIKNKRDIILIDEDNSAVNLLVSGVLSRKFEGVKSLAEIIAYEREKLGESTFIITTSSLDLLTAVADRAMYLEDHKAYYLDLKEFKEELKKYYLEIIDSFLNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ32</accession>
    <name>N6VQ32_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Prephenate dehydratase</fullName>
            <ecNumber evidence="8">4.2.1.51</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_05115</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="5">Amino-acid biosynthesis.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.51" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95995.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592157.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ32"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00466"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1003"/>
    <dbReference type="OrthoDB" id="8755at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004664">
        <property type="term" value="F:prephenate dehydratase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009094">
        <property type="term" value="P:L-phenylalanine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd04905">
        <property type="entry name" value="ACT_CM-PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd13532">
        <property type="entry name" value="PBP2_PDT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.190.10">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001086">
        <property type="entry name" value="Preph_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018528">
        <property type="entry name" value="Preph_deHydtase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008865">
        <property type="entry name" value="PRK11898.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022">
        <property type="entry name" value="PREPHENATE DEHYDRATASE P PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21022:SF19">
        <property type="entry name" value="PREPHENATE DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01842">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00800">
        <property type="entry name" value="PDT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53850">
        <property type="entry name" value="Periplasmic binding protein-like II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00858">
        <property type="entry name" value="PREPHENATE_DEHYDR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51171">
        <property type="entry name" value="PREPHENATE_DEHYDR_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="2" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="4 8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3" id="KW-0584">Phenylalanine biosynthesis</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Prephenate dehydratase" evidence="6">
        <location>
            <begin position="1"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="ACT" evidence="7">
        <location>
            <begin position="188"/>
            <end position="259"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023222"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51171"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95995.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="263" mass="30718" checksum="507D86040BE895D8" modified="2013-06-26" version="1">MIYTLPKGSYSEKAAKEFLKFLDGEYEIGYCNNIYEIFEKIDRSSLGVVPIENSIEGSVSLTLDLLLEFDVKILAEISLDIHHCLIGYDKEKIKIILSHPQALAQCRKYIKKHGWEVKAVESTIKAVELIEDERYGAIADRDVVKYYKHLKILDENIEDYKNNKTRFILIGYKKVKFKKEFKKYKTSIIFELKEDKPGALYHVLKEFAERGINLTRIESRPSKRRLGTYIFYIDYENGLEVLNSVKKHTTFIKVLGRYPVLVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQH6</accession>
    <name>N6VQH6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 16">Riboflavin kinase</fullName>
            <shortName evidence="16">RFK</shortName>
            <ecNumber evidence="3 16">2.7.1.161</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="13 16">CTP-dependent riboflavin kinase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="14 16">CTP:riboflavin 5'-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12 16">Flavokinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="16" type="primary">ribK</name>
        <name evidence="18" type="ORF">J422_04063</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">Catalyzes the CTP-dependent phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>riboflavin + CTP = CDP + FMN + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25021"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37563"/>
            <dbReference type="ChEBI" id="CHEBI:57986"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
            <dbReference type="EC" id="2.7.1.161"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="16">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="16">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 16">Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (CTP route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 16">Belongs to the archaeal riboflavin kinase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="16">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.1.161" evidence="3 16"/>
    <dbReference type="EMBL" id="APMM01000026">
        <property type="protein sequence ID" value="ENN96130.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591531.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQH6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00726"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.796"/>
    <dbReference type="OrthoDB" id="30955at2157"/>
    <dbReference type="UniPathway" id="UPA00276">
        <property type="reaction ID" value="UER00929"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008531">
        <property type="term" value="F:riboflavin kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009398">
        <property type="term" value="P:FMN biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009231">
        <property type="term" value="P:riboflavin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.30">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01285">
        <property type="entry name" value="Riboflavin_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053397">
        <property type="entry name" value="Archaeal_Riboflavin_Kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039063">
        <property type="entry name" value="RibK_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023470">
        <property type="entry name" value="Riboflavin_kinase_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023602">
        <property type="entry name" value="Riboflavin_kinase_CTP-dep"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023465">
        <property type="entry name" value="Riboflavin_kinase_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF040694">
        <property type="entry name" value="ribK_Meth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40706:SF1">
        <property type="entry name" value="RIBOFLAVIN KINASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01982">
        <property type="entry name" value="CTP-dep_RFKase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82114">
        <property type="entry name" value="Riboflavin kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 16" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="6 16" id="KW-0288">FMN</keyword>
    <keyword evidence="10 16 18" id="KW-0418">Kinase</keyword>
    <keyword evidence="11 16" id="KW-0460">Magnesium</keyword>
    <keyword evidence="8 16" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9 16" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7 16" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Riboflavin kinase" evidence="17">
        <location>
            <begin position="7"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="10"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="39"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="95"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="108"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>CDP</name>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005219"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006428"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011987"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00017394"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00029789"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00030544"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00033116"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF01982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ENN96130.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="126" mass="14549" checksum="D6AB33F6C00317C9" modified="2013-06-26" version="1">MIIKGKIISGKGEGRYYLSIKKYKKDIRKLLNFEPYEGTLNLKLNMDFNIENFNYLETEDFILNGKKYFGVRLLPVKIKVRDGVICGAIISPKKTDHGRDVIEIIAPIKLREKYNLKDGDVLEVVI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQM4</accession>
    <name>N6VQM4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Ferroxidase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_03563</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96195.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591242.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQM4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01390"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.695"/>
    <dbReference type="OrthoDB" id="4859at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008199">
        <property type="term" value="F:ferric iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008198">
        <property type="term" value="F:ferrous iron binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004322">
        <property type="term" value="F:ferroxidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042802">
        <property type="term" value="F:identical protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006879">
        <property type="term" value="P:intracellular iron ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006826">
        <property type="term" value="P:iron ion transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01055">
        <property type="entry name" value="Nonheme_Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.1260.10:FF:000001">
        <property type="entry name" value="Non-heme ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1260.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001519">
        <property type="entry name" value="Ferritin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012347">
        <property type="entry name" value="Ferritin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009040">
        <property type="entry name" value="Ferritin-like_diiron"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009078">
        <property type="entry name" value="Ferritin-like_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008331">
        <property type="entry name" value="Ferritin_DPS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041719">
        <property type="entry name" value="Ferritin_prok"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431:SF127">
        <property type="entry name" value="BACTERIAL NON-HEME FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11431">
        <property type="entry name" value="FERRITIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00210">
        <property type="entry name" value="Ferritin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47240">
        <property type="entry name" value="Ferritin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50905">
        <property type="entry name" value="FERRITIN_LIKE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-0408">Iron</keyword>
    <keyword evidence="1" id="KW-0409">Iron storage</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferritin-like diiron" evidence="5">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022434"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50905"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96195.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="170" mass="19716" checksum="8EB5D5087FB2BB8A" modified="2013-06-26" version="1">MIDKDIEKAINKQINSEFYSAYLYLSMAAYADSIGLKGFANWLMVQFKEETDHALKLYNYLLDRGGRVILDKIDKPKSEWNSILEVFEDGLKHEEEVTKSINELMDLAVAKKDYATINILQWFIDEQVEEEKSFGEILNKLKLIGNDKKALLMLDKDLAQRVYTPPTNEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQQ3</accession>
    <name>N6VQQ3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Glycosyl transferase family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_03498</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000022">
        <property type="protein sequence ID" value="ENN96230.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591151.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQQ3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01334"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.682"/>
    <dbReference type="OrthoDB" id="65872at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03801">
        <property type="entry name" value="GT4_PimA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028098">
        <property type="entry name" value="Glyco_trans_4-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050194">
        <property type="entry name" value="Glycosyltransferase_grp1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45947:SF3">
        <property type="entry name" value="SULFOQUINOVOSYL TRANSFERASE SQD2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13439">
        <property type="entry name" value="Glyco_transf_4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Glycosyltransferase subfamily 4-like N-terminal" evidence="2">
        <location>
            <begin position="17"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="1">
        <location>
            <begin position="184"/>
            <end position="345"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF13439"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96230.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="367" mass="42396" checksum="FD18C9100AC903A4" modified="2013-06-26" version="1">MKILMMSWKCIKHPWKGGAEFATYEILKRLSEKGYECVWFAPKFKGCLKEEEIEGIKIIRDFNIYTIYFNAWRKYVEEFKGKFDVVIDQVNGIPFFTPLYVKEPVIMYIHHVGDVCWDYEMPFPLSKIGKYSEKMVLRLYKNIPTVCVSPSTKKDLEDLGFKNINVVYNGCNVKPLDKLDFSIKEENSLCFVGRVTAMKRLEHAIKVVSLVKEEINDVKFYVVGRAKKEKYLCKLKNLANMLDLKNNVIFLGYLPFKERNEILRKSLIQIVTSVKEGWGLNVIEGNALGVPAVGYRVNGLVDSIKDGYNGFLVKDGNVKEMAEKVINILDDKNLLKKLSKNSIEWAKQFSWDKSAEEFEGVIKNVVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQY2</accession>
    <name>N6VQY2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="13">Purine NTPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">J422_03084</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96310.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590762.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQY2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00163"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.605"/>
    <dbReference type="OrthoDB" id="25344at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005085">
        <property type="term" value="F:guanyl-nucleotide exchange factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006302">
        <property type="term" value="P:double-strand break repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.510">
        <property type="entry name" value="Helix hairpin bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000219">
        <property type="entry name" value="DH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038729">
        <property type="entry name" value="Rad50/SbcC_AAA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013134">
        <property type="entry name" value="Zn_hook_RAD50"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32114:SF2">
        <property type="entry name" value="ABC TRANSPORTER ABCH.3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13476">
        <property type="entry name" value="AAA_23"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04423">
        <property type="entry name" value="Rad50_zn_hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75712">
        <property type="entry name" value="Rad50 coiled-coil Zn hook"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50010">
        <property type="entry name" value="DH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51131">
        <property type="entry name" value="ZN_HOOK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="7 10" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="3" id="KW-0227">DNA damage</keyword>
    <keyword evidence="8" id="KW-0234">DNA repair</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0862">Zinc</keyword>
    <feature type="domain" description="DH" evidence="11">
        <location>
            <begin position="166"/>
            <end position="449"/>
        </location>
    </feature>
    <feature type="domain" description="Zinc-hook" evidence="12">
        <location>
            <begin position="449"/>
            <end position="546"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="157"/>
            <end position="585"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="627"/>
            <end position="693"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="10">
        <location>
            <begin position="738"/>
            <end position="831"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="494"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="497"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022763"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00471"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="PROSITE" id="PS50010"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51131"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96310.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="907" mass="109656" checksum="0F68977EEDF9D119" modified="2013-06-26" version="1">MIIEKIKINNFKSHKNTEIEFKEGLTAIIGDNGSGKTSIFEAMVYALFGPNVVGGYDRIVTIGKRHVRVELTFRVRGNRVKVVREYENGRGGAYLYINNSLYARGVNEVNNKIKEILGVDQDIFLNSIYIKQGEIAKLINNLRNSERLKIIGKLLGIEDFETCYEKMKEVINEYNNRLREIEGELKSKILLENDLEKNKKILEEKYREKLKLKEKLEKLEEEFKVIDEKYREYLDRKEKFMNLTNKLELRKKELKILNEKLERLERDFEEFNKYENLLKENESSYYEYINIEKEINDLEKERNELEGKYKEYNSLLGKKEILERNIEKLKNEIKENLEDIKERLNYYENEINKLERIRSYIMELESLEKELNEIENQEIIRNKYKIYFDEYIKLSNELEELKNKQIEYYKLLEKKNSLIRQRNELIEEIEKLERKIGNIEELKRKLEKIEEVKKILEELNEKKNKLNEKLGELNNEIKRLKKMLDELKNVKGKCPLCKSEISEEKKAELIRINEMEIKSKKNEKECIEKELMDIDNKIKNLRNIIKEEPRVIEELKRHEILEKEIVRKKELIKELEKEINSIDLDKYIINNKPIDIIINEKKKRLEEIESFYNSYRLAEEFLKNKNKEELIKKINELRKEVGNRRLDDVEIEIKNLMKQRDIFKEKLSKYNQLLSLKEELKKICNNLKILKLDFDRYNEVINKLKIYREKERELREGYNKYSIAKDRIDKFKNVYGDKDKIIDEIEETKKRINEIVDKINYIKNRLDEINYSEEEFRKIENERNDKEEKLNSLKIRLVEIERDIDNLENNIKQIEERLNKLKEKEAEREKLLRFIDYLKRVRDIYSRGGFQRYLRKKFIPIIRNNLNKAFSEFELPYSFVDITDNFDIIVKSPNGDLTIENLSGGSR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="20" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR80</accession>
    <name>N6VR80_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">50S ribosomal protein L41e</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02504</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96410.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590453.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR80"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00771"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.490"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96410.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="52" mass="6695" checksum="54086804C9F54BBF" modified="2013-06-26" version="1">MLKKVVGLNKNKKEGEIIKRSSRRWKKKGRMRWKWYKKRLRRLKRERRRARS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR92</accession>
    <name>N6VR92_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Band 7 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_06638</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Single-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the band 7/mec-2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000056">
        <property type="protein sequence ID" value="ENN95666.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593809.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR92"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00367"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1292"/>
    <dbReference type="OrthoDB" id="10752at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0098552">
        <property type="term" value="C:side of membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.479.30:FF:000004">
        <property type="entry name" value="Putative membrane protease family, stomatin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.479.30">
        <property type="entry name" value="Band 7 domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043202">
        <property type="entry name" value="Band-7_stomatin-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001107">
        <property type="entry name" value="Band_7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036013">
        <property type="entry name" value="Band_7/SPFH_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018080">
        <property type="entry name" value="Band_7/stomatin-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001972">
        <property type="entry name" value="Stomatin_HflK_fam"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264:SF19">
        <property type="entry name" value="AT06885P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10264">
        <property type="entry name" value="BAND 7 PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01145">
        <property type="entry name" value="Band_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00721">
        <property type="entry name" value="STOMATIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00244">
        <property type="entry name" value="PHB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF117892">
        <property type="entry name" value="Band 7/SPFH domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01270">
        <property type="entry name" value="BAND_7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4" id="KW-1133">Transmembrane helix</keyword>
    <feature type="domain" description="Band 7" evidence="6">
        <location>
            <begin position="18"/>
            <end position="175"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004167"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="SMART" id="SM00244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95666.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="269" mass="30624" checksum="38A16498D6B07DCB" modified="2013-06-26" version="1">MIIFWIILGILVLIIITRSIVVVKQYEGGLIFRLGKVAGRLIPGINFIIPFIDVVQKVDLRTRVVDIPPQEMITKDNAVVRVDAVVYYRVIDVIKALLEVEDYEYAIINLAQTTLRAIIGSMELDEVLNKREYINSKLLEILDKETDPWGVRIEKVEVKEIDPPQDIKEAMAQQMKAERLKRAKILEAEGEKQSRILKAQGIAESLKIEAEGKAKAIQIVAEAARNYFKEEAQLYKALEVANNVLKENSKYIISENVLDLAKNIIKKRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRF5</accession>
    <name>N6VRF5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">N(4)-bis(aminopropyl)spermidine synthase</fullName>
            <ecNumber evidence="1">2.5.1.128</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="1">Branched-chain polyamine synthase A</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="1" type="primary">bpsA</name>
        <name evidence="3" type="ORF">J422_06055</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1">Involved in the biosynthesis of branched-chain polyamines, which support the growth of thermophiles under high-temperature conditions. Catalyzes the sequential condensation of spermidine with the aminopropyl groups of decarboxylated S-adenosylmethionines to produce N(4)-bis(aminopropyl)spermidine via N(4)-aminopropylspermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>2 S-adenosyl 3-(methylsulfanyl)propylamine + spermidine = N(4)-bis(aminopropyl)spermidine + 2 S-methyl-5'-thioadenosine + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:44132"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:82771"/>
            <dbReference type="EC" id="2.5.1.128"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Amine and polyamine biosynthesis.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1">Belongs to the branched-chain polyamine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.128" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593223.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRF5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00530"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1179"/>
    <dbReference type="OrthoDB" id="358909at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016765">
        <property type="term" value="F:transferase activity, transferring alkyl or aryl (other than methyl) groups"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006596">
        <property type="term" value="P:polyamine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01947">
        <property type="entry name" value="Aminopropyltransf_BpsA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014435">
        <property type="entry name" value="BpsA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002723">
        <property type="entry name" value="BpsA_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051720">
        <property type="entry name" value="rRNA_MeTrfase/Polyamine_Synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23290:SF0">
        <property type="entry name" value="RRNA N6-ADENOSINE-METHYLTRANSFERASE METTL5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01861">
        <property type="entry name" value="BpsA_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005895">
        <property type="entry name" value="UCP005895_mtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="1" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="N(4)-bis(aminopropyl)spermidine synthase C-terminal" evidence="2">
        <location>
            <begin position="105"/>
            <end position="349"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01947"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF01861"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="350" mass="40691" checksum="0F98B1EDD826F0C7" modified="2013-06-26" version="1">MQRIVEEVKSRIEIPVYERSIENVLSAILSTGDFWKIVDISEEPLPLVAEIIRVLEKEGLVKITDKIELTEKGLEFVKEYGIGKREDHICECCEGRGVSLKPYKDILEKFKEIAKDRPMPKHEYDQGYVKEECTVARIALMNSRGDLYNKDVLVLGDDDLTSIALMLSNLPKRIVVVDIDERLINFIKETAEKLNYKNIEIITLDLRNPLPEKYKKKFDTFITDPPETLYAIKTFIGRGISALKGERRAGYFGITRRESSLDKWREIQKILLNDFNVVITDLIRNFNQYINWGYEEETKAWRLAPIKKKPEDIWYKSYIFRIETLKGSKGFDEEVKVGDELYNDAESSTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRM4</accession>
    <name>N6VRM4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Endoribonuclease Nob1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_01890</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Toxic component of a type II toxin-antitoxin (TA) system. Processes pre-16S-rRNA at its 3' end (the D-site) to yield the mature 3' end.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the NOB1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96525.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590136.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRM4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00226"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.372"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030688">
        <property type="term" value="C:preribosome, small subunit precursor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004521">
        <property type="term" value="F:RNA endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030490">
        <property type="term" value="P:maturation of SSU-rRNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd09876">
        <property type="entry name" value="PIN_Nob1-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1010:FF:000020">
        <property type="entry name" value="20S-pre-rRNA D-site endonuclease NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.28.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039907">
        <property type="entry name" value="NOB1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033411">
        <property type="entry name" value="Ribonuclease_PIN"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR12814:SF2">
        <property type="entry name" value="RNA-BINDING PROTEIN NOB1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17146">
        <property type="entry name" value="PIN_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0690">Ribosome biogenesis</keyword>
    <keyword evidence="5" id="KW-1277">Toxin-antitoxin system</keyword>
    <feature type="domain" description="PIN" evidence="10">
        <location>
            <begin position="1"/>
            <end position="100"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005858"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00021078"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022517"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022649"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00045770"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96525.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="158" mass="18038" checksum="0C87AD232291B7EA" modified="2013-06-26" version="1">MKIVLDASAILHGYNPLTEDANHYITPEALEEIKSNRVIVDQAIRFGKLKIRSPKEEFINKVKETALKTGDRLSEQDVSVLALSLELKAILYTDDYAMQNVAKKLNINVKGIRYKTNRQIVWRKVCIGCKKIYPANYDEDECEICGSPLKRKAVKIKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRX2</accession>
    <name>N6VRX2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 9 10">Quinolinate synthase</fullName>
            <ecNumber evidence="2 9 10">2.5.1.72</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">nadA</name>
        <name evidence="11" type="ORF">J422_01223</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the condensation of iminoaspartate with dihydroxyacetone phosphate to form quinolinate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>iminosuccinate + dihydroxyacetone phosphate = quinolinate + phosphate + 2 H2O + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25888"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29959"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:57642"/>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
            <dbReference type="EC" id="2.5.1.72"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="9">Binds 1 [4Fe-4S] cluster per subunit.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from iminoaspartate: step 1/1.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the quinolinate synthase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.72" evidence="2 9 10"/>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96620.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589910.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRX2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00044"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.239"/>
    <dbReference type="OrthoDB" id="5931at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00327"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008987">
        <property type="term" value="F:quinolinate synthetase A activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0034628">
        <property type="term" value="P:'de novo' NAD+ biosynthetic process from L-aspartate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10800">
        <property type="entry name" value="NadA-like"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00568">
        <property type="entry name" value="NadA_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003473">
        <property type="entry name" value="NadA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036094">
        <property type="entry name" value="NadA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023066">
        <property type="entry name" value="Quinolinate_synth_type2"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00550">
        <property type="entry name" value="nadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006878">
        <property type="entry name" value="PRK09375.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573:SF0">
        <property type="entry name" value="QUINOLINATE SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30573">
        <property type="entry name" value="QUINOLINATE SYNTHETASE A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02445">
        <property type="entry name" value="NadA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142754">
        <property type="entry name" value="NadA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 9" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0408">Iron</keyword>
    <keyword evidence="8 9" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0662">Pyridine nucleotide biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="9">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="38"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="84"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="109"/>
            <end position="111"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="126"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="170"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="196"/>
            <end position="198"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="213"/>
        </location>
        <ligand>
            <name>iminosuccinate</name>
            <dbReference type="ChEBI" id="CHEBI:77875"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00012669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022642"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00568"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="NCBIfam" id="TIGR00550"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96620.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="305" mass="34494" checksum="9D27D9B7B0BBC5D8" modified="2013-06-26" version="1">MDIKDRILKLKEEKNAIILAHNYQPKEILKVADFMGDSLELCIKAKETSADIIVFCGVDFMAESAKILNPEKKVLMPEIKGTQCPMAHQLTKDIILKYKEKYDAPLVIYVNTTAEAKAMADITCTSANADKVVNSLSAETVLFGPDKNLAYYVQKRSDKKIIPIPENGGCYVHQSFTIEDVKKAKEKYPNAVLLVHPECNPEIQDVADVIASTSGMVKYVLNSDEEEFIIGTEKDLIGRIEIELEKLGEKKKLIPLSEKAVCKEMKQISLEKIERCLLEEKYEVKLDEEIIRRARKAIENMLKVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS15</accession>
    <name>N6VS15_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00991</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000006">
        <property type="protein sequence ID" value="ENN96670.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589866.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS15"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01020"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.193"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43122">
        <property type="entry name" value="FERREDOXIN SUBUNIT OF PYRUVATE:FLAVODOXIN OXIDOREDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43122:SF1">
        <property type="entry name" value="IRON-SULFUR-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="1"/>
            <end position="29"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="37"/>
            <end position="64"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96670.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="64" mass="7391" checksum="A09D3EB5A39B0B55" modified="2013-06-26" version="1">MIEINERFCKGCYICIFVCPKKVFEKSDKLNKKGIYPPIAKYPEKCSRCNLCVLQCPDQAISIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS63</accession>
    <name>N6VS63_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Putative DNA repair RAD25</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_05095</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95991.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592153.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS63"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00470"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.999"/>
    <dbReference type="OrthoDB" id="11644at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0140097">
        <property type="term" value="F:catalytic activity, acting on DNA"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0120545">
        <property type="term" value="F:nucleic acid conformation isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006935">
        <property type="entry name" value="Helicase/UvrB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014001">
        <property type="entry name" value="Helicase_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001650">
        <property type="entry name" value="Helicase_C-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050742">
        <property type="entry name" value="Helicase_Restrict-Modif_Enz"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47396:SF1">
        <property type="entry name" value="ATP-DEPENDENT HELICASE IRC3-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47396">
        <property type="entry name" value="TYPE I RESTRICTION ENZYME ECOKI R PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00271">
        <property type="entry name" value="Helicase_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04851">
        <property type="entry name" value="ResIII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00487">
        <property type="entry name" value="DEXDc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00490">
        <property type="entry name" value="HELICc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51192">
        <property type="entry name" value="HELICASE_ATP_BIND_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51194">
        <property type="entry name" value="HELICASE_CTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Helicase ATP-binding" evidence="1">
        <location>
            <begin position="29"/>
            <end position="172"/>
        </location>
    </feature>
    <feature type="domain" description="Helicase C-terminal" evidence="2">
        <location>
            <begin position="270"/>
            <end position="436"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51192"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95991.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="663" mass="78015" checksum="CDB7911E0FC61866" modified="2013-06-26" version="1">MIEDFNKLIKKCFGFTLKEYQKYVINNIIKNLERNKFIAVSMPTGSGKTIIEMFLAYYFKDKRILVLEPTRFLCDQMFKLWKTLFDNVEKDYEGKKFDYNKRIIISTPQTALKWARDFDVIIIDEIHHAFGNKYYKTLLSKLNPQYIFGFTALIIKEKRKEIGNVCFLDYDFKKLHQIDKTFSHPKAIIDIFDAELNIDEDKIYEKLFCCKVIGDVKAIKYLENVLIKYGKRAFCESLRNLLRKGKIEDKELIDFCKKGGISHKARAVLKVLKAYEVSQLKPVLIFTSRKATAKEFMEVIKNFKTELLTSDLSKDERLKLINRAKNGEVDIIISTLVGEEGIDIPESGLLIMTDIPKSPLRFYQRLGRLIRLSSKDKIKFLVFTLTPKTFEYDYLDEAINKLYNEGVDVSYILSDIEKTQKVKVVEIIKRYERPISYTKLILNEDIDPFEFYFNKVKEKLKGITSIEDESKLKKIFFILLTNPLYRGGLSKLIKDIDNYIISNNFSRSLDRAIERGEIYYIYNVNKLSKIIKFELLRLYNICLKRGIKKYENSLFKLDRKPFLRLMSDLFLEEDIRRVKYLLTTEDNIVSVGKIKYNKSSKSLYAKLIIPLCVNNIEIYLEVGISYFNIIKENEKSYKKFRELIKLNLKKAGSLAIEKFLKEI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS68</accession>
    <name>N6VS68_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Probable cyclic pyranopterin monophosphate synthase</fullName>
            <ecNumber evidence="3">4.6.1.17</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="3">Molybdenum cofactor biosynthesis protein C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="3" type="primary">moaC</name>
        <name evidence="5" type="ORF">J422_05120</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3">Catalyzes the conversion of (8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate to cyclic pyranopterin monophosphate (cPMP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:49580"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:59648"/>
            <dbReference type="ChEBI" id="CHEBI:131766"/>
            <dbReference type="EC" id="4.6.1.17"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 3">Cofactor biosynthesis; molybdopterin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homohexamer; trimer of dimers.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3">Belongs to the MoaC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.6.1.17" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95996.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592158.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS68"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00465"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1004"/>
    <dbReference type="OrthoDB" id="10067at2157"/>
    <dbReference type="UniPathway" id="UPA00344"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0061799">
        <property type="term" value="F:cyclic pyranopterin monophosphate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.640">
        <property type="entry name" value="Molybdopterin cofactor biosynthesis C (MoaC) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01224_A">
        <property type="entry name" value="MoaC_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023047">
        <property type="entry name" value="Mo_CF_biosynth-C_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023045">
        <property type="entry name" value="MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036522">
        <property type="entry name" value="MoaC_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050105">
        <property type="entry name" value="MoCo_biosynth_MoaA/MoaC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002820">
        <property type="entry name" value="Mopterin_CF_biosynth-C_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00581">
        <property type="entry name" value="moaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006870">
        <property type="entry name" value="PRK09364.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008999">
        <property type="entry name" value="PRK12343.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22960">
        <property type="entry name" value="MOLYBDOPTERIN COFACTOR SYNTHESIS PROTEIN A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01967">
        <property type="entry name" value="MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55040">
        <property type="entry name" value="Molybdenum cofactor biosynthesis protein C, MoaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0456">Lyase</keyword>
    <keyword evidence="2 3" id="KW-0501">Molybdenum cofactor biosynthesis</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Molybdopterin cofactor biosynthesis C (MoaC)" evidence="4">
        <location>
            <begin position="12"/>
            <end position="148"/>
        </location>
    </feature>
    <feature type="active site" evidence="3">
        <location>
            <position position="123"/>
        </location>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="72"/>
            <end position="74"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="3">
        <location>
            <begin position="108"/>
            <end position="109"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005046"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023150"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF01967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95996.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="150" mass="16891" checksum="15C9836819BDF0BC" modified="2013-06-26" version="1">MLTHVDDKGVKMVDISEKSEVIRVAEAEGYIKLKRETIELIKENKLEKGNVLTTAQIAGIMAVKKTHELIPLCHPLPITSVNIDFEVFEDKIKCICKVKAKYKTGVEMEALAGVSVALLTIWDMVKSVEKKDGYKETEIYGIKIIKKIKM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS87</accession>
    <name>N6VS87_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Transcriptional regulator GfcR</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">gfcR</name>
        <name evidence="6" type="ORF">J422_04725</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="domain">
        <text evidence="4">Contains an N-terminal DNA-binding winged helix-turn-helix domain and a C-terminal regulatory domain (or effector binding domain) resembling phosphoribosyltransferase (PRT) domain.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the purine/pyrimidine phosphoribosyltransferase family. GfcR subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96016.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591988.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS87"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00964"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.924"/>
    <dbReference type="OrthoDB" id="68893at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004588">
        <property type="term" value="F:orotate phosphoribosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019856">
        <property type="term" value="P:pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0010468">
        <property type="term" value="P:regulation of gene expression"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006222">
        <property type="term" value="P:UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06223">
        <property type="entry name" value="PRTases_typeI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2020">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01214">
        <property type="entry name" value="GfcR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022854">
        <property type="entry name" value="GfcR-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029057">
        <property type="entry name" value="PRTase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000836">
        <property type="entry name" value="PRTase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002620">
        <property type="entry name" value="PRK02277.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278">
        <property type="entry name" value="OROTATE PHOSPHORIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR19278:SF41">
        <property type="entry name" value="PYRE-LIKE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00156">
        <property type="entry name" value="Pribosyltran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53271">
        <property type="entry name" value="PRTase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="6" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0804">Transcription</keyword>
    <keyword evidence="1 4" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Phosphoribosyltransferase" evidence="5">
        <location>
            <begin position="59"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01214"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF00156"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96016.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="204" mass="22483" checksum="88E854ABBD12D0D0" modified="2013-06-26" version="1">MDKKLLEKVIELKKSGLTVGEIADELNVSMETARYLVLNAEKLLEKEEIKKNVDIFIDWKNIGSSAYRLRKISSIISDIIKSRKIEFDTVVGISNSGVPIATLVAEELDKELAIYIPKKQLLEDGKRIIGSISQNFSTISYKRAVIIDDVVTSGSTLTECVKQLKNSCSPKLIVVLIDKSGLDEIDGVPLVPLIRVGAVNIEGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS92</accession>
    <name>N6VS92_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Small GTP-binding protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04750</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96021.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591997.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS92"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00969"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.929"/>
    <dbReference type="OrthoDB" id="84946at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002098">
        <property type="term" value="P:tRNA wobble uridine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd00880">
        <property type="entry name" value="Era_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006073">
        <property type="entry name" value="GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714:SF6">
        <property type="entry name" value="TRANSLATION INITIATION FACTOR IF-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42714">
        <property type="entry name" value="TRNA MODIFICATION GTPASE GTPBP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01926">
        <property type="entry name" value="MMR_HSR1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00449">
        <property type="entry name" value="RASTRNSFRMNG"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="G" evidence="1">
        <location>
            <begin position="4"/>
            <end position="132"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96021.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="180" mass="20575" checksum="1A2C96687634CE4D" modified="2013-06-26" version="1">MEFKIAIIGPENAGKSSIMNALFGKYVSFVSEVGGTTKMPIKKYWGKIKIGRKKQPEFADLIFIDLGGLYTERDKESPLKPGVLKKTFEVIEDADMIIHVVDGKIGLLRNFERLHHLLKFRYQKPIIVVINKSDLLSEDDKIKIKKYIEERLKNEPLLISAKTGEGIEDLLNLIISYLKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSA8</accession>
    <name>N6VSA8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000029">
        <property type="protein sequence ID" value="ENN96036.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591918.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000029.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSA8"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.902"/>
    <dbReference type="OrthoDB" id="64574at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="44"/>
            <end position="67"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96036.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="73" mass="8259" checksum="7D01B7777D1571D1" modified="2013-06-26" version="1">MDEKRKLLFDKISNAGIVLVGYEFLFMLYIILNTASKTIAPNVGIILFVGDVIAIILTVWLFCAVLYDIYTKL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSB1</accession>
    <name>N6VSB1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">(NiFe)-hydrogenase-3-type complex Eha membrane protein EhaH</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00571</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96765.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589796.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSB1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00066"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.110"/>
    <dbReference type="OrthoDB" id="64568at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017059">
        <property type="entry name" value="NiFe-hyd_3_EhaH_prd"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10125">
        <property type="entry name" value="NADHdeh_related"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF036536">
        <property type="entry name" value="EhaH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="17"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="92"/>
            <end position="110"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="122"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="164"/>
            <end position="182"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="194"/>
            <end position="216"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96765.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="217" mass="23765" checksum="A4B87057BB82968C" modified="2013-06-26" version="1">MIELLGNIYALVPFGDIIFLFSDFSIIGFIIAIVFTIIVYLTKPEKQLEIQKFGEDVLVKIPLEELKIRRFMAIACGIATAGAMLTYDLFDFCLFLALIGITNIGIVSAVKKEHVLNAAYQYGLIAIISSLPLFASAGLVLAKTGTLSLLELNKINTNLIYEKILFSIGVAGETGVAPFYAAKAEMFRAPGAPYILMIHLSSLLLILRSVEILLSIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSB8</accession>
    <name>N6VSB8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_04223</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96046.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591721.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSB8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00678"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.826"/>
    <dbReference type="OrthoDB" id="17974at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040084">
        <property type="entry name" value="GTPase_Obg"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787">
        <property type="entry name" value="FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787:SF11">
        <property type="entry name" value="UPF0026 PROTEIN SLR1464"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01083">
        <property type="entry name" value="Uncharacterised_Radical_SAM_Su"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="7">
        <location>
            <begin position="6"/>
            <end position="233"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96046.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="307" mass="35344" checksum="868BE34733698EA9" modified="2013-06-26" version="1">MIVYGPVPSRRLGLSLGIDPVYYTCTFDCIYCQLGRTRYLVSSPKDIPKDVLEKFPTDEEIYNEVKKVIDKDIDYITFAGSGEPTLSPYLKEAIERLKEFNIPICVITNSSLMKYKKVRDALKEANLVSATLTSINQEMFKKIHRTEIKFKDVINGLIKFRKSAEDTVLNIELMVIEGINDDDKTIYKLKEIFEKINPNNIEINTPIRPPCEKYVKKVEYSRLVEIKNIIGDKAYIIGTCNKTYKKLENNLERISSMLKIRPCTVEDLANALGIHVSEVGKYLYILKDHGKLECKEVNGKKYYFLNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSE1</accession>
    <name>N6VSE1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">NADPH-dependent FMN reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_00040</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>FMN</name>
            <dbReference type="ChEBI" id="CHEBI:58210"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the SsuE family. Isf subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96795.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589701.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSE1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01159"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.8"/>
    <dbReference type="OrthoDB" id="9059at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.360">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029039">
        <property type="entry name" value="Flavoprotein-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005025">
        <property type="entry name" value="FMN_Rdtase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051796">
        <property type="entry name" value="ISF_SsuE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43278:SF3">
        <property type="entry name" value="IRON-SULFUR FLAVOPROTEIN MJ0731"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43278">
        <property type="entry name" value="NAD(P)H-DEPENDENT FMN-CONTAINING OXIDOREDUCTASE YWQN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03358">
        <property type="entry name" value="FMN_red"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52218">
        <property type="entry name" value="Flavoproteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0285">Flavoprotein</keyword>
    <keyword evidence="4" id="KW-0288">FMN</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NADPH-dependent FMN reductase-like" evidence="6">
        <location>
            <begin position="1"/>
            <end position="155"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022630"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00038292"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF03358"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96795.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="196" mass="21800" checksum="6042713AF9F301D1" modified="2013-06-26" version="1">MKVLGISGSPRLEGTHFAVNYALNYLKEKGAEVKYLTVHKKKINFCIHCDFCIKKREGCVFKDDMLEFYEAMEWADGIIIGTPVYQGTVSAQIKAVMDRTRAILAKNPNIFKNKVGMAIAVGGDRSGGQEIALRTIHDFFIINGIIPVSGGSFGANLGASLWSRDKGKEGVKEDEEGLRSLRKTLKRFLEMLEVRP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSG4</accession>
    <name>N6VSG4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Probable transcription termination protein NusA</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">nusA</name>
        <name evidence="10" type="ORF">J422_00160</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Participates in transcription termination.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the NusA family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96815.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589721.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSG4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01136"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.32"/>
    <dbReference type="OrthoDB" id="4116at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006353">
        <property type="term" value="P:DNA-templated transcription termination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031564">
        <property type="term" value="P:transcription antitermination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.300.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00945_A">
        <property type="entry name" value="NusA_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004087">
        <property type="entry name" value="KH_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015946">
        <property type="entry name" value="KH_dom-like_a/b"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004044">
        <property type="entry name" value="KH_dom_type_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009019">
        <property type="entry name" value="KH_sf_prok-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010212">
        <property type="entry name" value="NusA_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030842">
        <property type="entry name" value="TF_NusA_bacterial"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01952">
        <property type="entry name" value="nusA_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006261">
        <property type="entry name" value="PRK08406.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648">
        <property type="entry name" value="TRANSCRIPTION TERMINATION FACTOR NUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22648:SF0">
        <property type="entry name" value="TRANSCRIPTION TERMINATION_ANTITERMINATION PROTEIN NUSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07650">
        <property type="entry name" value="KH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00322">
        <property type="entry name" value="KH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54814">
        <property type="entry name" value="Prokaryotic type KH domain (KH-domain type II)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50084">
        <property type="entry name" value="KH_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="10" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="5 6" id="KW-0804">Transcription</keyword>
    <keyword evidence="4 6" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="1 6" id="KW-0806">Transcription termination</keyword>
    <feature type="domain" description="K Homology" evidence="9">
        <location>
            <begin position="31"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="8">
        <location>
            <begin position="159"/>
            <end position="178"/>
        </location>
    </feature>
    <feature type="compositionally biased region" description="Polar residues" evidence="8">
        <location>
            <begin position="168"/>
            <end position="178"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022472"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00945"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00322"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96815.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="178" mass="20302" checksum="1E7687B4D7E4AA94" modified="2013-06-26" version="1">MGKLRLTTEEIMKMGFFEKIANVPILDCVITDERVAFIVKEGDIGAAIGKGGEHVKEAEEKFGKKVDIIEYSNDWRKFIRNIFAPIKLDDVWVKRVGKDVVAFIKISPKVRKAVFGEKGKNLERALNILKRHTKITKIKVIINEKQQQKVNRNIKKEGKDIKEEGNINNTSQLAEAQN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSG5</accession>
    <name>N6VSG5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UPF0251 protein J422_04458</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_04458</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 2">Belongs to the UPF0251 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96091.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591814.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSG5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00632"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.874"/>
    <dbReference type="OrthoDB" id="74471at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00674">
        <property type="entry name" value="UPF0251"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013324">
        <property type="entry name" value="RNA_pol_sigma_r3/r4-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002852">
        <property type="entry name" value="UPF0251"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37478">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37478:SF2">
        <property type="entry name" value="UPF0251 PROTEIN TK0562"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02001">
        <property type="entry name" value="DUF134"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88659">
        <property type="entry name" value="Sigma3 and sigma4 domains of RNA polymerase sigma factors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009350"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00674"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96091.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="118" mass="13961" checksum="82ED1D3E3436ACED" modified="2013-06-26" version="1">MRGRKKLPRYISELPKFKIFKPAGKPLENLEKVKINIDELEAIRLVDYLDYTQEEAAKLMGISRRVLWNLLTEGRKKVADAIINGKALIIEENNYIIRECHQCIGHRFGWKKHCRWEL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSI2</accession>
    <name>N6VSI2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Peptidase, caspase catalytic subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04118</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000027">
        <property type="protein sequence ID" value="ENN96116.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591621.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000027.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSI2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01351"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.806"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.25.40.10">
        <property type="entry name" value="Tetratricopeptide repeat domain"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011990">
        <property type="entry name" value="TPR-like_helical_dom_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48452">
        <property type="entry name" value="TPR-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96116.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="385" mass="44040" checksum="D0D54089B6940729" modified="2013-06-26" version="1">MQNFELLEKSYELAKNIRDPYVRGNLLKDIAEGYCELNEFKKALEIVNEIDEESIRLEALNSLAIKLAKDREACKELLKRSFKLAKKYGKMEEYIITLAKIANTLAEVHESYKNVISEALKNTSLLKIDEKAKVLSDILGIIKDLDESREVVKEVLKIVEKLDEKERDGVLSTVILDLLKIGNIEKAIELSKRIKDHFWKTEAYIYIAYYLYKSKKQYKNYINMAVESAMNIKDEFWRDTALTKIAICLVKTGDYDQAIEISKAIKSDKALKSVVCGMSKNEMFNNALELAKEIKSNFWKALALSCIVRDLGMLGKSYDEVLNKVLKIADMIDNPVHKSIVLYNISCKIAKIGDEKKALEIVEKIEDTFWKAKALRDIAIIGGKQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSL3</accession>
    <name>N6VSL3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03853</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96161.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591370.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSL3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00402"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.755"/>
    <dbReference type="OrthoDB" id="59181at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96161.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="224" mass="26159" checksum="8756AE77CF27BF16" modified="2013-06-26" version="1">MKPKHALRKDMIGEFTLNKSFNTYRGKIIKADFNSPLEGIVMKNKKDQVYFYPLLALHMVKPLNCIPINVIPKTSLPTNPKNIHIKEALSRIVGRTLKVFYENPKTSYLGRLLGFTRGVFSWTLVLEIHGEVILLFNPDYIVYYGTRWDFLKNNPPYKPPKLMNVTKTANYLKRCLLEDVIIEPEYPRINIDNKVFVYPFGVIGKDEYLRETVETILKEKEFLN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSM5</accession>
    <name>N6VSM5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Large ribosomal subunit protein uL5</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="11" type="primary">rpl5p</name>
        <name evidence="7" type="synonym">rpl5</name>
        <name evidence="11" type="ORF">J422_03973</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">This is 1 of the proteins that bind and probably mediate the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. In the 70S ribosome it contacts protein S13 of the 30S subunit (bridge B1b), connecting the 2 subunits; this bridge is implicated in subunit movement. May contact the P site tRNA; the 5S rRNA and some of its associated proteins might help stabilize positioning of ribosome-bound tRNAs.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Part of the 50S ribosomal subunit; contacts the 5S rRNA and probably tRNA. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 7 8">Belongs to the universal ribosomal protein uL5 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96181.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591408.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSM5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00424"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.779"/>
    <dbReference type="OrthoDB" id="372044at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1440.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1440.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01333_A">
        <property type="entry name" value="Ribosomal_uL5_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002132">
        <property type="entry name" value="Ribosomal_uL5"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022804">
        <property type="entry name" value="Ribosomal_uL5_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031309">
        <property type="entry name" value="Ribosomal_uL5_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020929">
        <property type="entry name" value="Ribosomal_uL5_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022803">
        <property type="entry name" value="Ribosomal_uL5_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057266">
        <property type="entry name" value="Ribosomal_uL5_euk/arc-type"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031310">
        <property type="entry name" value="Ribosomal_uL5_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003258">
        <property type="entry name" value="PRK04219.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11994">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L11-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00281">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00673">
        <property type="entry name" value="Ribosomal_L5_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002161">
        <property type="entry name" value="Ribosomal_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55282">
        <property type="entry name" value="RL5-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00358">
        <property type="entry name" value="RIBOSOMAL_L5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 7 8" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="5 7 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="4 7" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="3 7" id="KW-0699">rRNA-binding</keyword>
    <keyword evidence="2 7" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL5 N-terminal" evidence="9">
        <location>
            <begin position="11"/>
            <end position="63"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL5 C-terminal" evidence="10">
        <location>
            <begin position="67"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003930"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00281"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00673"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96181.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="186" mass="21868" checksum="23FC7B8C7D826862" modified="2013-06-26" version="1">MSFEELWEKQPMLRPRIEKVVVNFGVGESGDRLVKGEKVIQELTGQKPIKTRAKQTNPTFGIRKKLPIGLKVTLRGKRAEEFLRNAFEAFKKEGKKLYDYSFDEYGNFSFGIHEHIDFPGQKYDPMIGVFGMDVCVTLERPGFRVKRRKRCKAKIPRRHRLTKEEAIEFIEKTFGIKVERWEEEVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSW1</accession>
    <name>N6VSW1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Acetylglutamate kinase</fullName>
            <ecNumber evidence="9">2.7.2.8</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="9">N-acetyl-L-glutamate 5-phosphotransferase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="9">NAG kinase</fullName>
            <shortName evidence="9">NAGK</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="9" type="primary">argB</name>
        <name evidence="11" type="ORF">J422_02979</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the ATP-dependent phosphorylation of N-acetyl-L-glutamate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-acetyl-L-glutamate + ATP = N-acetyl-L-glutamyl 5-phosphate + ADP</text>
            <dbReference type="Rhea" id="RHEA:14629"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:44337"/>
            <dbReference type="ChEBI" id="CHEBI:57936"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.2.8"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 9">Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 2/4.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the acetylglutamate kinase family. ArgB subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.2.8" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96291.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590742.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSW1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00143"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.584"/>
    <dbReference type="OrthoDB" id="6816at2157"/>
    <dbReference type="UniPathway" id="UPA00068">
        <property type="reaction ID" value="UER00107"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003991">
        <property type="term" value="F:acetylglutamate kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042450">
        <property type="term" value="P:L-arginine biosynthetic process via ornithine"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd04250">
        <property type="entry name" value="AAK_NAGK-C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1160.10:FF:000004">
        <property type="entry name" value="Acetylglutamate kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1160.10">
        <property type="entry name" value="Acetylglutamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00082">
        <property type="entry name" value="ArgB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036393">
        <property type="entry name" value="AceGlu_kinase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004662">
        <property type="entry name" value="AcgluKinase_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037528">
        <property type="entry name" value="ArgB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001048">
        <property type="entry name" value="Asp/Glu/Uridylate_kinase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041727">
        <property type="entry name" value="NAGK-C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00761">
        <property type="entry name" value="argB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23342:SF0">
        <property type="entry name" value="N-ACETYLGLUTAMATE SYNTHASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00696">
        <property type="entry name" value="AA_kinase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000728">
        <property type="entry name" value="NAGK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53633">
        <property type="entry name" value="Carbamate kinase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 9" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="3 9" id="KW-0055">Arginine biosynthesis</keyword>
    <keyword evidence="8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="6 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 9 11" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/glutamate/uridylate kinase" evidence="10">
        <location>
            <begin position="20"/>
            <end position="268"/>
        </location>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <begin position="60"/>
            <end position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="25"/>
        </location>
    </feature>
    <feature type="site" description="Transition state stabilizer" evidence="9">
        <location>
            <position position="248"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004828"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022571"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00082"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00696"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96291.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="292" mass="31955" checksum="407FA889EAC9AC2C" modified="2013-06-26" version="1">MNKAKVLLEALPYIQKFHNKIFVIKYGGHAMIDENAKNYTAQDIVLLKYVGIKPVIVHGGGPEINRAMEKFGKKPEFIHGLRVTDEETLNIVEMVLTGKINGEIVSRIFKFGGKAVGLSGKSGRIILAKKKRKFIEKDGKKVEVDLGRVGETVEINTELLDILINNNYIPVISPIGIDEKGDAYNLNADTVAGDIASALKAEKLILITDVDGIMEDINDPSTVYKKLTVSDVKRLLKEGKIKGGMIPKVECAIKALESGGVKSVHIINGKIPHALLVEIFTEEGIGTMITKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTF8</accession>
    <name>N6VTF8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">6-phospho 3-hexuloisomerase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02240</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the SIS family. PHI subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96471.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590278.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTF8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01196"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.440"/>
    <dbReference type="OrthoDB" id="350569at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097367">
        <property type="term" value="F:carbohydrate derivative binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016853">
        <property type="term" value="F:isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:1901135">
        <property type="term" value="P:carbohydrate derivative metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd05005">
        <property type="entry name" value="SIS_PHI"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10490">
        <property type="entry name" value="Glucose-6-phosphate isomerase like protein, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017552">
        <property type="entry name" value="PHI/rmpB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001347">
        <property type="entry name" value="SIS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046348">
        <property type="entry name" value="SIS_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03127">
        <property type="entry name" value="RuMP_HxlB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43443">
        <property type="entry name" value="3-HEXULOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43443:SF1">
        <property type="entry name" value="3-HEXULOSE-6-PHOSPHATE ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01380">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53697">
        <property type="entry name" value="SIS domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51464">
        <property type="entry name" value="SIS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SIS" evidence="2">
        <location>
            <begin position="25"/>
            <end position="159"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51464"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96471.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="172" mass="19260" checksum="344142D3EB534031" modified="2013-06-26" version="1">MELNIILKNISSLENILNSEAVEDMINRILKANRIFIFGVGRSGYVGRCFAMRLIHLGLKAYFVGETVTPAYKKEDLLILISGSGKTEAVINVAKKAKKINNNIIAVVCKYGDLHKLADLTITLDVKKSNYLPLGTAFEEKAMIFFDLIVAELMKRLNKSEDDLLNEHCNLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTY2</accession>
    <name>N6VTY2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Elongation factor 1-alpha</fullName>
            <shortName evidence="11">EF-1-alpha</shortName>
            <ecNumber evidence="11">3.6.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="11">Elongation factor Tu</fullName>
            <shortName evidence="11">EF-Tu</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="11" type="primary">tuf</name>
        <name evidence="13" type="ORF">J422_01288</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="11">GTP hydrolase that promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15 16">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
            <dbReference type="EC" id="3.6.5.3"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>GTP + H2O = GDP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19669"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58189"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="10">
            <dbReference type="Rhea" id="RHEA:19670"/>
        </physiologicalReaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 11">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.5.3" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000008">
        <property type="protein sequence ID" value="ENN96631.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589921.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000008.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTY2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00032"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.252"/>
    <dbReference type="OrthoDB" id="371718at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003924">
        <property type="term" value="F:GTPase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003746">
        <property type="term" value="F:translation elongation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01883">
        <property type="entry name" value="EF1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03693">
        <property type="entry name" value="EF1_alpha_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd03705">
        <property type="entry name" value="EF1_alpha_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000003">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.30.10:FF:000005">
        <property type="entry name" value="Elongation factor 1-alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.300:FF:000204">
        <property type="entry name" value="Translation elongation factor Tu"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.30.10">
        <property type="entry name" value="Translation factors"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00118_A">
        <property type="entry name" value="EF_Tu_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004161">
        <property type="entry name" value="EFTu-like_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR031157">
        <property type="entry name" value="G_TR_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054696">
        <property type="entry name" value="GTP-eEF1A_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005225">
        <property type="entry name" value="Small_GTP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000795">
        <property type="entry name" value="T_Tr_GTP-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050100">
        <property type="entry name" value="TRAFAC_GTPase_members"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009000">
        <property type="entry name" value="Transl_B-barrel_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009001">
        <property type="entry name" value="Transl_elong_EF1A/Init_IF2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004539">
        <property type="entry name" value="Transl_elong_EF1A_euk/arc"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00483">
        <property type="entry name" value="EF-1_alpha"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF008969">
        <property type="entry name" value="PRK12317.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00231">
        <property type="entry name" value="small_GTP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23115">
        <property type="entry name" value="TRANSLATION FACTOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22594">
        <property type="entry name" value="GTP-eEF1A_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00009">
        <property type="entry name" value="GTP_EFTU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03144">
        <property type="entry name" value="GTP_EFTU_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00315">
        <property type="entry name" value="ELONGATNFCT"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50465">
        <property type="entry name" value="EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50447">
        <property type="entry name" value="Translation proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00301">
        <property type="entry name" value="G_TR_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51722">
        <property type="entry name" value="G_TR_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 11" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 11 13" id="KW-0251">Elongation factor</keyword>
    <keyword evidence="9 11" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="6 11 13" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 11" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="8 11" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Tr-type G" evidence="12">
        <location>
            <begin position="5"/>
            <end position="225"/>
        </location>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="14"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <position position="21"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="91"/>
            <end position="95"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="11">
        <location>
            <begin position="149"/>
            <end position="152"/>
        </location>
        <ligand>
            <name>GTP</name>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022768"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022917"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00049117"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96631.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="428" mass="47298" checksum="73CF5D8186FE6824" modified="2013-06-26" version="1">MAKQKPVLNVAFIGHVDAGKSTTVGRLLYDSGAIDPQELEKLKREAQERGKAGFEFAYVMDKLKEERERGVTIDVAHKKFETNKYEVTIVDCPGHRDFIKNMITGASQADAAILVVDVNDAKTGIQPQTREHMFLARTLGIKQIAVCINKMDTVNYSQEEYEKMKKMLSEQLLKILGYDPSKIDFIPTASLKGDNVVKRSENMPWYKGPTLVEAIDKFQPPEKPVNLPLRIPIQDVYSITGVGTVPVGRVETGVLKVGDKVVFEPAGVSGEVKSIEMHHEPIQVAEPGDNIGFNVRGVSKKDIKRGDVCGHPDNPPTVAEEFTAQIVVLQHPTAITVGYTPVFHAHTAQVACTFIELLKKLDPRTGQVIEENPQFLKTGDAAIVRVKPTKPMVIENVREIPQLGRFAIRDMGMTIAAGMAIDVKPKNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU02</accession>
    <name>N6VU02_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF354 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01141</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96656.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589896.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU02"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00814"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.223"/>
    <dbReference type="OrthoDB" id="185087at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007152">
        <property type="entry name" value="DUF354"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39662:SF1">
        <property type="entry name" value="DUF354 DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39662">
        <property type="entry name" value="DUF354 DOMAIN-CONTAINING PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04007">
        <property type="entry name" value="DUF354"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005357">
        <property type="entry name" value="UCP005357"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96656.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="333" mass="38542" checksum="84FB6DEF474C13AF" modified="2013-06-26" version="1">MSVWIDLTNAPHVHFFIQLIRKLEREGIDYFITYRMSKHLKELINLYNIPAICVGKHGKDIKDKLIQYANRVKKLSKIISKINPKVAVAKHSVELPRVAFGLNIKTIFVVDNEYAEAQNRLTLPLVDKIIKPIPTNKWLLEKYGGREFLEFDGVCEMANVNARLKNIDHNILKRLNISNDKPIIIMRPCPNSSYCNEKDILPEIIKEIKKRFDCYIIAFPRTKKQEILYKKLNTIVPKTLDNISLLYHSNAMIGAGGTMNRESAIIGITTVSCYPKELLGVDKYLIEKGRMIHTFDISEIIDYLENNLDKRKKVLKLEDPTEMIFKEICKVIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU20</accession>
    <name>N6VU20_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Small ribosomal subunit protein eS31</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">rps27ae</name>
        <name evidence="8" type="ORF">J422_00881</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="6">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="6">Binds 1 zinc ion per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 30S ribosomal subunit.</text>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the eukaryotic ribosomal protein eS31 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96681.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589846.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU20"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00598"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.173"/>
    <dbReference type="OrthoDB" id="25142at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990904">
        <property type="term" value="C:ribonucleoprotein complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005840">
        <property type="term" value="C:ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.20.50.180">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00777">
        <property type="entry name" value="Ribosomal_eS31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002906">
        <property type="entry name" value="Ribosomal_eS31"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022845">
        <property type="entry name" value="Ribosomal_eS31_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011332">
        <property type="entry name" value="Ribosomal_zn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001669">
        <property type="entry name" value="PRK00432.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01599">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01402">
        <property type="entry name" value="Ribosomal_S27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57829">
        <property type="entry name" value="Zn-binding ribosomal proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 8" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0862">Zinc</keyword>
    <keyword evidence="2 6" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="Small ribosomal subunit protein eS31" evidence="7">
        <location>
            <begin position="9"/>
            <end position="51"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="45"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="48"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM01402"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96681.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="61" mass="7244" checksum="322CDA9811B68F9E" modified="2013-06-26" version="1">MTKGKKVAKYKYYKIEGDKVVRLRRFCPRCGPGVFLAEHLNRYSCGKCGYLEWKQPQKKEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU47</accession>
    <name>N6VU47_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Tungsten-containing formylmethanofuran dehydrogenase 2 subunit C</fullName>
            <ecNumber evidence="3">1.2.7.12</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Tungsten-containing formylmethanofuran dehydrogenase II subunit C</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_00711</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N-formylmethanofuran + 2 oxidized [2Fe-2S]-[ferredoxin] + H2O = methanofuran + 2 reduced [2Fe-2S]-[ferredoxin] + CO2 + H(+)</text>
            <dbReference type="Rhea" id="RHEA:19841"/>
            <dbReference type="Rhea" id="RHEA-COMP:10000"/>
            <dbReference type="Rhea" id="RHEA-COMP:10001"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16526"/>
            <dbReference type="ChEBI" id="CHEBI:33737"/>
            <dbReference type="ChEBI" id="CHEBI:33738"/>
            <dbReference type="ChEBI" id="CHEBI:57727"/>
            <dbReference type="ChEBI" id="CHEBI:58151"/>
            <dbReference type="EC" id="1.2.7.12"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">One-carbon metabolism; methanogenesis from CO(2); 5,10-methenyl-5,6,7,8-tetrahydromethanopterin from CO(2): step 1/3.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the FwdC/FmdC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.2.7.12" evidence="3"/>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96716.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589816.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU47"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00894"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.137"/>
    <dbReference type="OrthoDB" id="106216at2157"/>
    <dbReference type="UniPathway" id="UPA00640">
        <property type="reaction ID" value="UER00692"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0018493">
        <property type="term" value="F:formylmethanofuran dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019386">
        <property type="term" value="P:methanogenesis, from carbon dioxide"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="CDD" id="cd00980">
        <property type="entry name" value="FwdC/FmdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.160.20.60">
        <property type="entry name" value="Glutamate synthase, alpha subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054942">
        <property type="entry name" value="FMH_DH_FwdC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017550">
        <property type="entry name" value="Formylmethanofuran_DH_suC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036485">
        <property type="entry name" value="Glu_synth_asu_C_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF042910">
        <property type="entry name" value="FMH_DH_FwdC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03122">
        <property type="entry name" value="one_C_dehyd_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39673">
        <property type="entry name" value="TUNGSTEN FORMYLMETHANOFURAN DEHYDROGENASE, SUBUNIT C (FWDC)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39673:SF5">
        <property type="entry name" value="TUNGSTEN-CONTAINING FORMYLMETHANOFURAN DEHYDROGENASE 2 SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF69336">
        <property type="entry name" value="Alpha subunit of glutamate synthase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0484">Methanogenesis</keyword>
    <keyword evidence="6" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004830"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006761"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00012692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022994"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00047194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047203"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048228"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96716.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="273" mass="29736" checksum="11B93B53568BA502" modified="2013-06-26" version="1">MKELILELKKELVSPVEMDPVLPEKIESMKIDEIKDIKLYCGKKIVKLEDIFDIELNEIDSEPRIIIKNSTMKLKYIGAKMTKGEIVVEGDAGIGVGAEMKGGKIIVEGNADSWAGMNLKGGELIIKGNAKNYLGSSYRGDWRGMSGGKIIVEGNIGSEAGEFMNGGEIHIKGNAGMYLGIHQNGGIIFVNGNVGPRLGGEMKAGAIVVYGEVEDLLPSFKYEGIVENPVIKLKKKDPGVKVEGKFYKLTGDFVNEKPKGQLYISVDKNPNLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU63</accession>
    <name>N6VU63_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00831</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96736.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589836.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU63"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00868"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.164"/>
    <dbReference type="OrthoDB" id="63269at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96736.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="234" mass="26596" checksum="6B9B74F04CF5326A" modified="2013-06-26" version="1">MVLGKIKELPVNAQIITKVKVVDIRERESEDGSIFYIGTIVDKDGVANFITNIPLEKGKCYEIFGRVTDEKSVKIIEKVIKGIKYPREIPEIPKEELFNRGEVFDVKVPAILEISSDNIYINYYCSICNSLVEAKIKPKGLVYICKNCGELDPEEVIVRIKALGKIHFGTVSKRCYIPFSTLEKYMPRILEILEEFGVEDTIKDIAIKLHGKTFLVRGFDGKDGNYIITELEDI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="54" xmlns="https://uniprot.org/uniprot">
    <accession>N6VUC1</accession>
    <name>N6VUC1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Formate-dependent phosphoribosylglycinamide formyltransferase</fullName>
            <ecNumber evidence="7">6.3.1.21</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">5'-phosphoribosylglycinamide transformylase 2</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Formate-dependent GAR transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">GAR transformylase 2</fullName>
            <shortName evidence="7">GART 2</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Non-folate glycinamide ribonucleotide transformylase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="7">Phosphoribosylglycinamide formyltransferase 2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 10" type="primary">purT</name>
        <name evidence="10" type="ORF">J422_00045</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7">Involved in the de novo purine biosynthesis. Catalyzes the transfer of formate to 5-phospho-ribosyl-glycinamide (GAR), producing 5-phospho-ribosyl-N-formylglycinamide (FGAR). Formate is provided by PurU via hydrolysis of 10-formyl-tetrahydrofolate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="7">
            <text>N(1)-(5-phospho-beta-D-ribosyl)glycinamide + formate + ATP = N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:24829"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.1.21"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="7">Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (formate route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the PurK/PurT family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.21" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96796.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589702.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VUC1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01158"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.9"/>
    <dbReference type="OrthoDB" id="9299at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00127"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043815">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase 2 activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004644">
        <property type="term" value="F:phosphoribosylglycinamide formyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01643">
        <property type="entry name" value="PurT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003135">
        <property type="entry name" value="ATP-grasp_carboxylate-amine"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005862">
        <property type="entry name" value="PurT"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054350">
        <property type="entry name" value="PurT/PurK_preATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048740">
        <property type="entry name" value="PurT_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011054">
        <property type="entry name" value="Rudment_hybrid_motif"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF006766">
        <property type="entry name" value="PRK09288.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01142">
        <property type="entry name" value="purT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43055">
        <property type="entry name" value="FORMATE-DEPENDENT PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43055:SF1">
        <property type="entry name" value="FORMATE-DEPENDENT PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02222">
        <property type="entry name" value="ATP-grasp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF21244">
        <property type="entry name" value="PurT_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22660">
        <property type="entry name" value="RS_preATP-grasp-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51246">
        <property type="entry name" value="Rudiment single hybrid motif"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 7 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="2 7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3 7 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 7" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="ATP-grasp" evidence="9">
        <location>
            <begin position="116"/>
            <end position="303"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="20"/>
            <end position="21"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="80"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="111"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="157"/>
            <end position="162"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="191"/>
            <end position="194"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="199"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="262"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="274"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="281"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="349"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="356"/>
            <end position="357"/>
        </location>
        <ligand>
            <name>N(1)-(5-phospho-beta-D-ribosyl)glycinamide</name>
            <dbReference type="ChEBI" id="CHEBI:143788"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01643"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96796.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="385" mass="43076" checksum="D0E213687DEC55FA" modified="2013-06-26" version="1">MIGTPLLKGATKFLILGSGELGKEIVIEAQRLGIECVAVDRYQNAPAMQVAHRSYVINMRDYDALFSIIEREEPDFVIPEIEAINIDALLDAESFTEVIPNAEAVKITMNRELLRRLAAEKLKLKTAKYEFATNLEELKEAVEKLGYPCVVKPIMSSSGKGQSIIKDEKDIEKAYNKAKEARGSSEKLIVEEFIDFDYEITLLTAKSEDIKFCEPIGHIQKDGDYIMSWQPHNMPDELKNEAKNIAEKIVSALGGRGIFGVEMFVKNDEVIFSEVSPRPHDTGMVTMITQEMSEFEIHVRSILTLPISTKLLTPGASYAIKANINKYAPKYDIKDALKVENVKIRLFGKPNATIGRRMGVILAHAENVDKAKELVKKAAELIKIC</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="47" xmlns="https://uniprot.org/uniprot">
    <accession>N6VUD0</accession>
    <name>N6VUD0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Phosphoglucosamine mutase</fullName>
            <ecNumber evidence="7 9">5.4.2.10</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">glmM</name>
        <name evidence="15" type="ORF">J422_00115</name>
    </gene>
    <organism evidence="15 16">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="15 16" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="15 16">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">Catalyzes the conversion of glucosamine-6-phosphate to glucosamine-1-phosphate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:23424"/>
            <dbReference type="ChEBI" id="CHEBI:58516"/>
            <dbReference type="ChEBI" id="CHEBI:58725"/>
            <dbReference type="EC" id="5.4.2.10"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="9">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="9">Binds 1 Mg(2+) ion per subunit.</text>
    </comment>
    <comment type="PTM">
        <text evidence="9">Activated by phosphorylation.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 9 10">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="15">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.4.2.10" evidence="7 9"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96806.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589712.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VUD0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01145"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.23"/>
    <dbReference type="OrthoDB" id="10363at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008966">
        <property type="term" value="F:phosphoglucosamine mutase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03087">
        <property type="entry name" value="PGM_like1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.120.10:FF:000002">
        <property type="entry name" value="Phosphoglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.310.50:FF:000009">
        <property type="entry name" value="Probable phosphoglucosamine mutase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.120.10">
        <property type="entry name" value="Alpha-D-Glucose-1,6-Bisphosphate, subunit A, domain 3"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01554_A">
        <property type="entry name" value="GlmM_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005844">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016055">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-I/II/III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005845">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-II"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005846">
        <property type="entry name" value="A-D-PHexomutase_a/b/a-III"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005843">
        <property type="entry name" value="A-D-PHexomutase_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036900">
        <property type="entry name" value="A-D-PHexomutase_C_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016066">
        <property type="entry name" value="A-D-PHexomutase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005841">
        <property type="entry name" value="Alpha-D-phosphohexomutase_SF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023666">
        <property type="entry name" value="GlmM_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024086">
        <property type="entry name" value="GlmM_arc-type"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03990">
        <property type="entry name" value="Arch_GlmM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43771:SF1">
        <property type="entry name" value="PHOSPHOMANNOMUTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02878">
        <property type="entry name" value="PGM_PMM_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02879">
        <property type="entry name" value="PGM_PMM_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02880">
        <property type="entry name" value="PGM_PMM_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00408">
        <property type="entry name" value="PGM_PMM_IV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00509">
        <property type="entry name" value="PGMPMM"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55957">
        <property type="entry name" value="Phosphoglucomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53738">
        <property type="entry name" value="Phosphoglucomutase, first 3 domains"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00710">
        <property type="entry name" value="PGM_PMM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0413">Isomerase</keyword>
    <keyword evidence="4 9 10" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 9 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="2 9" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="16" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="12">
        <location>
            <begin position="2"/>
            <end position="123"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="13">
        <location>
            <begin position="145"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase alpha/beta/alpha" evidence="14">
        <location>
            <begin position="246"/>
            <end position="351"/>
        </location>
    </feature>
    <feature type="domain" description="Alpha-D-phosphohexomutase C-terminal" evidence="11">
        <location>
            <begin position="360"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="active site" description="Phosphoserine intermediate" evidence="9">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <feature type="binding site" description="via phosphate group" evidence="9">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="229"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="9">
        <location>
            <position position="231"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="modified residue" description="Phosphoserine" evidence="9">
        <location>
            <position position="89"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010231"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022553"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00050364"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00066330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00068193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01554"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU004326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF00408"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="Pfam" id="PF02878"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="Pfam" id="PF02879"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="14">
        <source>
            <dbReference type="Pfam" id="PF02880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="EMBL" id="ENN96806.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="16">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="441" mass="49344" checksum="839C7F09557F26FF" modified="2013-06-26" version="1">MKLFGTSGIRMRNLSPKIAYKVGSALVSLGYEDIVIAKDTRTTGKLIESAIIAGILNSGGEVTKIGIAPTPVLGFNSREYDAGVMITASHNPPEYNGIKMFNKNGVAFNKYEEEKIERVIFDNNLKYVNWNEVGEVFDDSKAIKRYRDFILNNVDLEKSYNVVVDCANGAGCLVSPYLLADLNCKVISLNSHIDGRFSGRLPEPDEKNLKETMKVVEALGYIGIAHDGDADRAVIIDEKGRLADFDKILALFCNYIVEKTKTKKIVTTIDASMIIDEILDGKAEVIRTKVGDVFVAEKMLEEGAVFGGEPSGTWIHGDIHLTPDGILTGLRVLEMLDYYDEKLYKLLDRLPSYFNIREKLPCKEEDKVKVINYIIEKGEEVFKTKPETVDGVRFNLEDGWILIRPSGTEEFIRIRVEAKSREKAEELLDKGVKLVKEALKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VWY9</accession>
    <name>N6VWY9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_06842</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95617.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594242.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VWY9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01079"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1327"/>
    <dbReference type="OrthoDB" id="18492at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03217">
        <property type="entry name" value="ABC_FeS_Assembly"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010230">
        <property type="entry name" value="FeS-cluster_ATPase_SufC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204">
        <property type="entry name" value="ABC TRANSPORTER I FAMILY MEMBER 6, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43204:SF2">
        <property type="entry name" value="ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="3"/>
            <end position="237"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95617.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="238" mass="27031" checksum="5E13A07AEACD4026" modified="2013-06-26" version="1">MLLKVEELYLKKGNRQILNGVNLEVNENEIHAIIGPNGAGKSSLAYTLMGVSGYEPYKGKIVFKNVDITNKPIYERARMGLTLAWQEPARFEGITVKKYLKIGMNKKYLNNPEDKIREALDLVGLKCNKYMERFVDDSLSGGERKRIELASIICMEPDLAILDEPDSGIDMVSIDEILNVFEYLKEQGCSLLVITHREEMAKNADRTSLICNGEVILTGDPKKVSEFYRRRCGKCKFR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX62</accession>
    <name>N6VX62_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Ribosomal L11 methyltransferase</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06436</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95702.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593537.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX62"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01288"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1253"/>
    <dbReference type="OrthoDB" id="106720at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042054">
        <property type="term" value="F:histone methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016274">
        <property type="term" value="F:protein-arginine N-methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025799">
        <property type="entry name" value="Arg_MeTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021172">
        <property type="entry name" value="UCP006607_RNA_methylase-rel"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11006:SF4">
        <property type="entry name" value="PROTEIN ARGININE N-METHYLTRANSFERASE 7"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06325">
        <property type="entry name" value="PrmA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006607">
        <property type="entry name" value="RNAmts_UCP006607"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95702.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="259" mass="29814" checksum="30F4A186D5053062" modified="2013-06-26" version="1">MILELDAPQWHLSLLHDYERIAIYKKAIEENVKESDIVFDLGTGSGILAMIAALKAKRVYAIELDSFTYEYAKENIKRNGFDNIILIEGDAELYNFKENPDVIVAELLDTALIAESHVRVMNAMIKKGYINDNTKLIPKGVINTVQLVEAKFNHIYYDEKSEAKPLSEEMIYEEVSFYKINPLKMKYKFKFHLDEDYNNVGLRLKTYTILDDKHVAGPTPMLNPPLVIPVGRAKRGDLLIKLFYERGVFESIKVRAYDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="52" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX71</accession>
    <name>N6VX71_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2 10">Transcription initiation factor IIB</fullName>
            <shortName evidence="10">TFIIB</shortName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">tfb</name>
        <name evidence="13" type="ORF">J422_06486</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9 10">Stabilizes TBP binding to an archaeal box-A promoter. Also responsible for recruiting RNA polymerase II to the pre-initiation complex (DNA-TBP-TFIIB).</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 10">Belongs to the TFIIB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95712.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593556.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX71"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01278"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1262"/>
    <dbReference type="OrthoDB" id="7429at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097550">
        <property type="term" value="C:transcription preinitiation complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003700">
        <property type="term" value="F:DNA-binding transcription factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0017025">
        <property type="term" value="F:TBP-class protein binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003743">
        <property type="term" value="F:translation initiation factor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070897">
        <property type="term" value="P:transcription preinitiation complex assembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd20549">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd20550">
        <property type="entry name" value="CYCLIN_TFIIB_archaea_like_rpt2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.10:FF:000023">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.472.170:FF:000001">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.25.10:FF:000037">
        <property type="entry name" value="Transcription initiation factor IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.170">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.472.10">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00383">
        <property type="entry name" value="TF2B_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013763">
        <property type="entry name" value="Cyclin-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036915">
        <property type="entry name" value="Cyclin-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000812">
        <property type="entry name" value="TFIIB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023484">
        <property type="entry name" value="TFIIB_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023486">
        <property type="entry name" value="TFIIB_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013150">
        <property type="entry name" value="TFIIB_cyclin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013137">
        <property type="entry name" value="Znf_TFIIB"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001658">
        <property type="entry name" value="PRK00423.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618:SF13">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11618">
        <property type="entry name" value="TRANSCRIPTION INITIATION FACTOR IIB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00382">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08271">
        <property type="entry name" value="Zn_Ribbon_TF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00685">
        <property type="entry name" value="TIFACTORIIB"/>
    </dbReference>
    <dbReference type="SMART" id="SM00385">
        <property type="entry name" value="CYCLIN"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF47954">
        <property type="entry name" value="Cyclin-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57783">
        <property type="entry name" value="Zinc beta-ribbon"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00782">
        <property type="entry name" value="TFIIB"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51134">
        <property type="entry name" value="ZF_TFIIB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="13" id="KW-0396">Initiation factor</keyword>
    <keyword evidence="3 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="13" id="KW-0648">Protein biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10" id="KW-0677">Repeat</keyword>
    <keyword evidence="8 10" id="KW-0804">Transcription</keyword>
    <keyword evidence="7 10" id="KW-0805">Transcription regulation</keyword>
    <keyword evidence="6 10" id="KW-0862">Zinc</keyword>
    <keyword evidence="5 11" id="KW-0863">Zinc-finger</keyword>
    <feature type="domain" description="TFIIB-type" evidence="12">
        <location>
            <begin position="18"/>
            <end position="49"/>
        </location>
    </feature>
    <feature type="repeat" description="1" evidence="10">
        <location>
            <begin position="135"/>
            <end position="218"/>
        </location>
    </feature>
    <feature type="repeat" description="2" evidence="10">
        <location>
            <begin position="229"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="22"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="25"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="41"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="44"/>
        </location>
        <ligand>
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00010857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00013932"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023015"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00053882"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00469"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN95712.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="318" mass="35813" checksum="D2D03FFE129EC667" modified="2013-06-26" version="1">MEVKEKLEKKEKKDEQEEEIVCPICGSKEVIKDYVKAEIVCSKCGCVIKDNLFDVGPEWRAFDHEQKIKRCRVGAPITLTIHDKGLSTVIDWRNKDSYGKDISANKRAQLYRLRKWQRRIRVSDASERNLTFALSELDRIASKLGLPRHVREHAAKIYRNAVEKGLIRGRSIEGVVAAAVYAACRKCKIPRTLDEIAEVSRVDRKEIGRTYRFLARELGIKLTPTNPIDYVPRFASELGLPGEVESKAIQILKKAAEKGLTSGRGPTGVAAAAIYIASVLLGCRKTQREVAEVAGVTEVTIRNRYKELTEHLDIDVTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX81</accession>
    <name>N6VX81_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">YibE/F family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06194</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95722.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593331.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX81"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00299"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1206"/>
    <dbReference type="OrthoDB" id="380833at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012507">
        <property type="entry name" value="YibE_F"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41771:SF1">
        <property type="entry name" value="MEMBRANE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR41771">
        <property type="entry name" value="MEMBRANE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07907">
        <property type="entry name" value="YibE_F"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="106"/>
            <end position="125"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="132"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="157"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="182"/>
            <end position="204"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="287"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="328"/>
            <end position="351"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95722.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="355" mass="39573" checksum="3A77AB4C2470783F" modified="2013-06-26" version="1">MKNKIYIIFLFAILLLSVILVVINYNYKNDYSIKKGVVIKTYDNIDYTAPIKIGFQIVYVNVSGNIIKFNNVLRGNFLLDNYFKKGDHVVVVYKDNTPIYLAYDRVFWIIMFSILFLGLLFLIGGFNGFKTVLSLIFTVVVIYYILLPNIANGTPPITLTIIIILLIVFISYFILCGFNKKAICSALGAIFGVVISGIVGYIGINLMNLPPGGFSEFVEMILFSGHYLDFYGVFISSILLASLGAIMDVSIDISSGLYELKRHKPDISFKEMFKSGMNIGRDIMGTMANTLILVYVGSLMAPILYFIIKNTPMEIIFSYNIIASEVLASLAGSIGVVMTVPITVILASYLYTKVE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXE1</accession>
    <name>N6VXE1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">CDP-glucose 4,6-dehydratase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05903</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000046">
        <property type="protein sequence ID" value="ENN95792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592963.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000046.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXE1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00991"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1151"/>
    <dbReference type="OrthoDB" id="4907at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd05252">
        <property type="entry name" value="CDP_GD_SDR_e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.25.10">
        <property type="entry name" value="UDP-galactose 4-epimerase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013445">
        <property type="entry name" value="CDP_4_6_deHydtase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016040">
        <property type="entry name" value="NAD(P)-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02622">
        <property type="entry name" value="CDP_4_6_dhtase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43000">
        <property type="entry name" value="DTDP-D-GLUCOSE 4,6-DEHYDRATASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16363">
        <property type="entry name" value="GDP_Man_Dehyd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD(P)-binding" evidence="1">
        <location>
            <begin position="15"/>
            <end position="330"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="366" mass="42459" checksum="4166A1C5D61EEFBD" modified="2013-06-26" version="1">MENKLSEFFKGKKILITGHTGFKGSWLTQILLNFGADVVGYSLKPNTSPNLFKILKLDKKIKNYYADIRDFDKIKSIMEKEKPEIVFHLAAQPLVRDSYDDPLYTYETNIIGTANILQAVKDVGGVKAAIMITTDKVYENKEWIWAYRENDELGGYDPYSTSKACAELIIRSYIRSFFNVKDYGKKHNTLIASVRAGNVVGGGDWSKDRLVPDIIRAIFEGDGTVVLRNPESIRPWQHVLEPLLGYLLLAERLYNGEIDFIGAWNLAPNEENFITVEELVKKGTEILGKGRYIIKKDEEKHESKILKLDATKAKVYLGWRPLLSIDETLKWTFEWYRRYYNGEDMITFTNRQINTFFERWERARRH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXP1</accession>
    <name>N6VXP1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">PUA domain containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_05364</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="pathway">
        <text evidence="1">tRNA modification; archaeosine-tRNA biosynthesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the archaeosine synthase type 1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95907.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592390.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXP1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01314"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1047"/>
    <dbReference type="OrthoDB" id="115061at2157"/>
    <dbReference type="UniPathway" id="UPA00393"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002099">
        <property type="term" value="P:tRNA wobble guanine modification"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd21149">
        <property type="entry name" value="PUA_archaeosine_TGT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.130.10">
        <property type="entry name" value="PUA domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.105">
        <property type="entry name" value="Queuine tRNA-ribosyltransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10630">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050076">
        <property type="entry name" value="ArchSynthase1/Queuine_TRR"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040777">
        <property type="entry name" value="DUF5591"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002478">
        <property type="entry name" value="PUA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015947">
        <property type="entry name" value="PUA-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036974">
        <property type="entry name" value="PUA_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036511">
        <property type="entry name" value="TGT-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004521">
        <property type="entry name" value="Uncharacterised_CHP00451"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036895">
        <property type="entry name" value="Uracil-DNA_glycosylase-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00451">
        <property type="entry name" value="unchar_dom_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499:SF2">
        <property type="entry name" value="ARCHAEOSINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46499">
        <property type="entry name" value="QUEUINE TRNA-RIBOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17884">
        <property type="entry name" value="DUF5591"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01472">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00359">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88697">
        <property type="entry name" value="PUA domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51713">
        <property type="entry name" value="tRNA-guanine transglycosylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52141">
        <property type="entry name" value="Uracil-DNA glycosylase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50890">
        <property type="entry name" value="PUA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="PUA" evidence="4">
        <location>
            <begin position="449"/>
            <end position="516"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005030"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008906"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="SMART" id="SM00359"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN95907.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="516" mass="60191" checksum="4F4CB42C710D747B" modified="2013-06-26" version="1">MLEPIAYDIGRLCKFKDSFTPNLIDIDIKIDDPKMPFDCPLELKDKFKKSFFGEIDYNGERITYQVLNYGKYVDEIELMDVDLYIIADRRIIERKELQIIKDIRGKISPNSGIYVPATLPWEIPLLAYMGVDFFDDSLAQFYASMGYRLTKNRAIKEDSNNLLEKNKEVYKEILSEVRLAIKNGYLRNLVEETSISHPYLWANYLRYKPDNRNIPLVKDNKIIITANIKVPEVEKYLERLNYEPYSNVIVLLPCSAKKPYSKSKSHRRFIEAIKRSKAVVDEIIITSPFFLVPRALENLVNYDIPVTGKWSYEEIEGINKYLKNFINTVEEKFNEYIIIAFLPEHYLEILDIDCIKIESNKLNELTETLKKYKDMTKDLSYKGRKIHYLKELCKFQFGYNFLPDEIRVNNRGQIFYKNIQLCSIGRNSLLVLTLHGGKRLWEILGRRSNYVEVSYNIKPGSLFPPGFVDCNENIGYRDEVVLIKDDEFVGVGRALMSGAEMKKAKHGALVNIRKVV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXY9</accession>
    <name>N6VXY9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DUF1156 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04655</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96002.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591954.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXY9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00949"/>
    <dbReference type="REBASE" id="68616">
        <property type="entry name" value="M.MviT80ORF4655P"/>
    </dbReference>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.910"/>
    <dbReference type="OrthoDB" id="93530at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003676">
        <property type="term" value="F:nucleic acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002052">
        <property type="entry name" value="DNA_methylase_N6_adenine_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009537">
        <property type="entry name" value="DUF1156"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014455">
        <property type="entry name" value="N6_adenine_Mtase_MK1259"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06634">
        <property type="entry name" value="DUF1156"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF009427">
        <property type="entry name" value="UCP009427_DNAmts"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00092">
        <property type="entry name" value="N6_MTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF1156" evidence="1">
        <location>
            <begin position="14"/>
            <end position="60"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF06634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96002.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="989" mass="114690" checksum="D9781839A7B23AA9" modified="2013-06-26" version="1">MEDIERRFIENPKFPIREINEKSEKEKGPARPPYWEMVFYWTRKPLIGVRSVIAGALLPYDIGVERFKKLVCLDENTPHRFNPRIPNDLKKYFEGKKLLDPFAGFGSIPLEGLRLGLEVTAVELLPTTYIFLKAVLEYPKKFGKSLIKDVEKWGNWITEQLKNDPEIKELYDEDVAVYIGTWEIKCPHCGKWTPLIGNYWLARVKDNKKGYKRLAFMKAVKKEDKVDIEVVDVNKIAKENNLSLNEAKVDKNKIKIGDLELVVPNANIEPKRNQAICLYCGNIIKFADKVGNHYIEKPKNKDVDFYVKFALRKYHEGDESFARQRLLVKVKIKDRDLEFEPATEEDNIKLEKAKEKVRKLIEKGDPDVPSEPISYYSVRYLFPILYGMTEWYKLFNPRQLLTLVKIVKLIREVGKRVEEEKLKEGWDKEKAFEYAEAIATYLSVALINYVDFNSMQTYWEVVWLTNKRTIAVRGISMMWNWCDVVPTSALAVGSFVKSIQKELRGIDYLTSALAPSSQKTLTDFTKTNTIKVLQGDATSLNLGEKFDLIVTDPPYADDVPYTELSDFYYVWLKRALSDVEDNKLVPRFHKEAFFKMIGKKYKEIKTQWQEFAKREVSKNSGRFMNENNKNKVAEKHFENLFSQAFISMKNHLKDDGLLITYYAHTNPESWATLLEAGWKRAKLTITRAIPLSTESKQSIVSRGKLSLDTSIIAVWKKKTLKDKILISNLIPKLREKGKETAELLIKHGQHDLDLLYGVMAGILEEVTQYNEIVSPEGKLTTKDILERYIYPLTIYSIIDAISKEKEGTLKILSKPGLFYTTYKILFGNKTLGSGDLILLNISTGIDARNAVEYKLIKRDDKGFVLNSPDLIKELDEKYLYQFLIEKNINPTNPEIKTSIDILHLLEYYAFAYPISTFKSNLEELRNKYPTETEEAINIAKLINKYYTSVFAKLYSGKKDSEIDKQLKKDKIYELYLIRRLVRFLEGKAF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VY54</accession>
    <name>N6VY54_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Phosphomevalonate dehydratase large subunit</fullName>
            <ecNumber evidence="9">4.2.1.182</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_04228</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of a hydro-lyase that catalyzes the dehydration of mevalonate 5-phosphate (MVA5P) to form trans-anhydromevalonate 5-phosphate (tAHMP). Involved in the archaeal mevalonate (MVA) pathway, which provides fundamental precursors for isoprenoid biosynthesis, such as isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(R)-5-phosphomevalonate = (2E)-3-methyl-5-phosphooxypent-2-enoate + H2O</text>
            <dbReference type="Rhea" id="RHEA:78975"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:58146"/>
            <dbReference type="ChEBI" id="CHEBI:229665"/>
            <dbReference type="EC" id="4.2.1.182"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="5">
            <dbReference type="Rhea" id="RHEA:78976"/>
        </physiologicalReaction>
    </comment>
    <comment type="pathway">
        <text evidence="1">Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via mevalonate pathway.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Heterodimer composed of a large subunit (PMDh-L) and a small subunit (PMDh-S).</text>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the AcnX type II large subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="4.2.1.182" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96047.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591722.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VY54"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00677"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.827"/>
    <dbReference type="OrthoDB" id="25253at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016829">
        <property type="term" value="F:lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008299">
        <property type="term" value="P:isoprenoid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01355">
        <property type="entry name" value="AcnX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007506">
        <property type="entry name" value="PMDh-L-like_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36577">
        <property type="entry name" value="DUF521 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G00490)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36577:SF3">
        <property type="entry name" value="DUF521 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_6G00490)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04412">
        <property type="entry name" value="AcnX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0414">Isoprene biosynthesis</keyword>
    <keyword evidence="4" id="KW-0456">Lyase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Phosphomevalonate dehydratase large subunit-like" evidence="11">
        <location>
            <begin position="1"/>
            <end position="395"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005092"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023229"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00045120"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00045299"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00046333"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00046520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00047176"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00047196"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF04412"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96047.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="395" mass="44239" checksum="33545C8AE1907829" modified="2013-06-26" version="1">MYLTKEEERILDGEYGEIKQKCLELLVKLGEIYGAERLINISSAQISGVSYKTIGDIGLEFLEDFSKEKVAVYSTLNPAGMDLDNWKELKIKEDFAEKQLRIIEAFKRMGVEISCTCTPYLTGNIPSFSEHIAWAESSAVSFANSVLGARTNREGAQSALASAILGKTPYYGYHLDENRMADFIVKIDAEKNNNDETFFSAVGYIVGEIAKNRVPYFKDLYKYNPSKDDLKSLCAVMAASGGVALYHAENITPECKVKNVVDDKVEKIEIDKKEIKDAYDRLNTTDEEPELICIGCPHCSIREIKEIAEILKDKKLKADLWVCCSLHVKSISDRMGFTKIIEKANGKVVKDTCMVVSPIEDNYKIVATNSGKAAVYLPSFCKSEVIFKSIRELIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VY61</accession>
    <name>N6VY61_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04253</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96052.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591732.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VY61"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00672"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.832"/>
    <dbReference type="OrthoDB" id="65467at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96052.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="83" mass="9562" checksum="85ADD16C847C467A" modified="2013-06-26" version="1">MIYGVLLNIPEKYAPKYEDIIRKIIGEGIARGDIISFCEARYKGDVAFVMLARSRRAAQKVYEELKEHPIHVKVIEIEGKEIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VY90</accession>
    <name>N6VY90_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Alpha-amylase AmyA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_04353</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 57 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96072.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VY90"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00652"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.853"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd10795">
        <property type="entry name" value="GH57N_MJA1_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.110.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052046">
        <property type="entry name" value="GH57_Enzymes"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011330">
        <property type="entry name" value="Glyco_hydro/deAcase_b/a-brl"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004300">
        <property type="entry name" value="Glyco_hydro_57_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36306:SF1">
        <property type="entry name" value="ALPHA-AMYLASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36306">
        <property type="entry name" value="ALPHA-AMYLASE-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03065">
        <property type="entry name" value="Glyco_hydro_57"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88713">
        <property type="entry name" value="Glycoside hydrolase/deacetylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0119">Carbohydrate metabolism</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycoside hydrolase family 57 N-terminal" evidence="3">
        <location>
            <begin position="3"/>
            <end position="284"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006821"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF03065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96072.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="405" mass="48467" checksum="919A13D0420A7EEF" modified="2013-06-26" version="1">MITFNFEVHQPHRLNKEIKASENLFDRYVDKKLNKEIFNRVAEKCYIPTNELILELIDDYDFKVNYSISGVFIEQAIEFNEYVLDLFKDLVDTGNVELIAETYHHSLTSLYEDEEEFINDIEMHRELYKDIFNYRAKVFRNTELIYNNRIAKIAKDLGFEAIFTEGIERILGWRSPNYLYVSPDNMKVLLRNYRLSDDIGFRFSARDWDQYPLTADKYAIWLAATPGEVINIYIDYETFGEHHWKESGIFEFLRYLPYEISKYEHLEVANVSEVLEKLEPKGEIFVHEFSTISWADTERDVSAWLGNRMQIISFEKLKEIGKMIKENKDKLGNKYYEIYKMYKILETSDNFYYQCTKGLSDMDVHNYFSHFNTPFDAYACYLNILYDFEYYIKQLLNKENNLLIA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VY96</accession>
    <name>N6VY96_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">A-type ATP synthase subunit C</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">atpC</name>
        <name evidence="8" type="ORF">J422_04413</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Component of the A-type ATP synthase that produces ATP from ADP in the presence of a proton gradient across the membrane.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Has multiple subunits with at least A(3), B(3), C, D, E, F, H, I and proteolipid K(x).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cell membrane</location>
            <topology evidence="6">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the V-ATPase V0D/AC39 subunit family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96082.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591796.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VY96"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00641"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.865"/>
    <dbReference type="OrthoDB" id="4272at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033179">
        <property type="term" value="C:proton-transporting V-type ATPase, V0 domain"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046933">
        <property type="term" value="F:proton-transporting ATP synthase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046961">
        <property type="term" value="F:proton-transporting ATPase activity, rotational mechanism"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0042777">
        <property type="term" value="P:proton motive force-driven plasma membrane ATP synthesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.132.50">
        <property type="entry name" value="ATP synthase (C/AC39) subunit, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.1690.10">
        <property type="entry name" value="V-type ATP synthase subunit C domain"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00314">
        <property type="entry name" value="ATP_synth_C_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036079">
        <property type="entry name" value="ATPase_csu/dsu_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014272">
        <property type="entry name" value="ATPase_V0-cplx_csu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002843">
        <property type="entry name" value="ATPase_V0-cplx_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050873">
        <property type="entry name" value="V-ATPase_V0D/AC39_subunit"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035067">
        <property type="entry name" value="V-type_ATPase_csu/dsu"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044911">
        <property type="entry name" value="V-type_ATPase_csu/dsu_dom_3"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02923">
        <property type="entry name" value="AhaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002267">
        <property type="entry name" value="PRK01198.1-3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38682">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38682:SF1">
        <property type="entry name" value="V-TYPE ATP SYNTHASE SUBUNIT C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01992">
        <property type="entry name" value="vATP-synt_AC39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103486">
        <property type="entry name" value="V-type ATP synthase subunit C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0066">ATP synthesis</keyword>
    <keyword evidence="6" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 6" id="KW-0375">Hydrogen ion transport</keyword>
    <keyword evidence="8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4 6" id="KW-0406">Ion transport</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 6" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="24"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006709"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022781"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023310"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00314"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96082.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="374" mass="43175" checksum="85B36EE6B7B8970F" modified="2013-06-26" version="1">MEFLTILLMAAIVIIVLTVIVWITKMVIDLAPYAYVNARVRSREARLLDRNKINEILEAQSFDELIGILEDTDYSEYISKVSGKDPIFIEKAFDLYLANLYKFLYDIAPNKAKQVLKIFMKKFDIKNIKTLIRAKNINLDAEEISDLLLPFGNIPKEKLRELSEVKTVEEVIRGLEGTEYFKVLQEALTEYEHTKNIIYLELALDKYYLNSLKRVMMIEGKEEDTFREFIGRMIDIENLKVILKTKADGLSGEEVVKYLTSGYELAEWKLKDLANATNIEAVLGGLEGTKYAEILSECMDEYNREKSIYVFEKILDKYLLELGKKLSLRKPFGIGPIIGLIVSKELEIKNLKAIIKGKMENMKAEEIKNFLIYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYJ2</accession>
    <name>N6VYJ2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="7">Desulfoferrodoxin ferrous iron-binding region</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_03548</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the desulfoferrodoxin family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96192.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591236.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYJ2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01393"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.692"/>
    <dbReference type="OrthoDB" id="30725at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005506">
        <property type="term" value="F:iron ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.730">
        <property type="entry name" value="SOR catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002742">
        <property type="entry name" value="Desulfoferrodoxin_Fe-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036073">
        <property type="entry name" value="Desulfoferrodoxin_Fe-bd_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051233">
        <property type="entry name" value="Desulfoferrodoxin_SOR"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00332">
        <property type="entry name" value="neela_ferrous"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36541">
        <property type="entry name" value="SUPEROXIDE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36541:SF1">
        <property type="entry name" value="SUPEROXIDE REDUCTASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01880">
        <property type="entry name" value="Desulfoferrodox"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF49367">
        <property type="entry name" value="Superoxide reductase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0249">Electron transport</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="Desulfoferrodoxin ferrous iron-binding" evidence="6">
        <location>
            <begin position="10"/>
            <end position="107"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005941"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96192.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="115" mass="13631" checksum="EB5CDA9AB6185213" modified="2013-06-26" version="1">MIVNVNKNNTDFERKHAPKIECKDVVKVNEIYEVKLYMEVEHPMEKEHYIQYIELYAEDYPLAKIFLTPYAKPEVTLKIKAPSEGHEGREFRLIAYAYCNLHGIWKSEKKIKIEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYK5</accession>
    <name>N6VYK5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylaminoimidazole-succinocarboxamide synthase</fullName>
            <ecNumber evidence="8">6.3.2.6</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">SAICAR synthetase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purC</name>
        <name evidence="10" type="ORF">J422_03633</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="7 8">
            <text>5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate + ATP = (2S)-2-[5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:22628"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58443"/>
            <dbReference type="ChEBI" id="CHEBI:77657"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.2.6"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 8">Belongs to the SAICAR synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.2.6" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96207.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591264.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYK5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01377"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.709"/>
    <dbReference type="OrthoDB" id="10775at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00131"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004639">
        <property type="term" value="F:phosphoribosylaminoimidazolesuccinocarboxamide synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01415">
        <property type="entry name" value="SAICAR_synt_PurC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.470.20:FF:000006">
        <property type="entry name" value="Phosphoribosylaminoimidazole-succinocarboxamide synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.200.20">
        <property type="entry name" value="Phosphorylase Kinase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00137">
        <property type="entry name" value="SAICAR_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028923">
        <property type="entry name" value="SAICAR_synt/ADE2_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033934">
        <property type="entry name" value="SAICAR_synt_PurC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001636">
        <property type="entry name" value="SAICAR_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050089">
        <property type="entry name" value="SAICAR_synthetase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018236">
        <property type="entry name" value="SAICAR_synthetase_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00081">
        <property type="entry name" value="purC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43599">
        <property type="entry name" value="MULTIFUNCTIONAL PROTEIN ADE2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43599:SF3">
        <property type="entry name" value="SI:DKEY-6E2.2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01259">
        <property type="entry name" value="SAICAR_synt"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56104">
        <property type="entry name" value="SAICAR synthase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01057">
        <property type="entry name" value="SAICAR_SYNTHETASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 8 10" id="KW-0436">Ligase</keyword>
    <keyword evidence="4 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="SAICAR synthetase/ADE2 N-terminal" evidence="9">
        <location>
            <begin position="12"/>
            <end position="236"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004672"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00010190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00048475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00137"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF01259"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96207.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="240" mass="27663" checksum="01D5502101586D90" modified="2013-06-26" version="1">MDISEILKKEPIHKGKAKTVYEIDEDKVLIEFRDDITAGNGEKKDVIKNKGYLNALISAKLFETLEKNGVATHYIKYIEPRYIVAKRVDIIPIEIIVRNIAAGSLCRRYPFKEGQVLNSPIVQYDYKNDEYKDPMINEDIAVALGLATREELKKMREIALKVNDILKKYFDEKGIILVDFKIEVGRDREGNLLVADEISPDTMRLWDKETKEPLDKDVFRKDLGDVLAKYKKVAERMGLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYN3</accession>
    <name>N6VYN3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">CRISPR-associated protein, Csx11 family</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03423</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000021">
        <property type="protein sequence ID" value="ENN96237.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591063.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000021.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYN3"/>
    <dbReference type="OrthoDB" id="137774at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96237.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="64" mass="7498" checksum="96D29940AB670DA1" modified="2013-06-26" version="1">MELEGEGLLFWAEKQGNKARIYLISSIRDFLKRFGNEEIRKALKNKDNEKLNSILSSKEEIKNI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYS0</accession>
    <name>N6VYS0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Acetyl-CoA decarbonylase/synthase complex subunit epsilon</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03201</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000019">
        <property type="protein sequence ID" value="ENN96277.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590893.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000019.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYS0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00317"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.628"/>
    <dbReference type="OrthoDB" id="120588at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019385">
        <property type="term" value="P:methanogenesis, from acetate"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1220">
        <property type="entry name" value="TPP-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003704">
        <property type="entry name" value="CdhB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029035">
        <property type="entry name" value="DHS-like_NAD/FAD-binding_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00315">
        <property type="entry name" value="cdhB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02552">
        <property type="entry name" value="CO_dh"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52467">
        <property type="entry name" value="DHS-like NAD/FAD-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96277.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="141" mass="16580" checksum="3F1BDEDB48796222" modified="2013-06-26" version="1">MDRFTPYIITAGSNTNHAEVISPSALKMMIKRARSPILILGEKCEREIIDRLIETYKLKVIKTPEEMNLMIVMKYLTQSEHDLAIFVGITYYYLAQALMHLKHFSNLVTVSIDRYYQPNAHYSFPNLDEKEYLDYLNRLIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYT1</accession>
    <name>N6VYT1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">TatD-related deoxyribonuclease</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_02959</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the metallo-dependent hydrolases superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96287.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590738.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYT1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00139"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.580"/>
    <dbReference type="OrthoDB" id="359310at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016788">
        <property type="term" value="F:hydrolase activity, acting on ester bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018228">
        <property type="entry name" value="DNase_TatD-rel_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001130">
        <property type="entry name" value="TatD-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012022">
        <property type="entry name" value="UCP005295"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42658">
        <property type="entry name" value="HYDROLASE TATD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42658:SF1">
        <property type="entry name" value="HYDROLASE TATD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01026">
        <property type="entry name" value="TatD_DNase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005295">
        <property type="entry name" value="UCP005295_TatD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01137">
        <property type="entry name" value="TATD_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="PIRNR" id="PIRNR005295"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96287.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="251" mass="29082" checksum="133A9E92CF2B4B94" modified="2013-06-26" version="1">MIDAHTHLDVRSFEDLEKMALSGIEVIITCSHNPYKMSTPEVYLDHWDRLINLEVKRGEMAGVEVKVALGIHPMGYPKNWKILIKELPKFLDNENVVAIGEAGLHYLTEDEKNLLREQLILAKDYDMPIILHTPEKNKREALLEILKILDEIKIKDNLVMIDHINKETVNLIDKNIYIGLTVQPSMKITHKEAAEIIKDYNKNFILSSDLGSLKSDIYALPRTKLYMKKIDVEEKKIVDATYRNAKKFYRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ02</accession>
    <name>N6VZ02_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Roadblock/LC7 family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02714</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96357.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590594.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ02"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00095"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.532"/>
    <dbReference type="OrthoDB" id="60411at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96357.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="186" mass="21586" checksum="93BAD3BA3B16A6EE" modified="2013-06-26" version="1">MLSTIVFLLSFCIGTVLGIYVKIKREKNIEKNHKELENKVIENLKELKKYVAPLDDREYSKEYDLVEIALDYGLEDIVVVNDEGLVIASTLKDGEMLGASGLDIYERIKKYHDDTKKVIIYKDNLYTYIYRGKLNNERIYIIIDSKTQIDKFNEIEILGKIAKILRKQYMAPLENIESISGEIIKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ07</accession>
    <name>N6VZ07_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Nickel import system ATP-binding protein NikD</fullName>
            <ecNumber evidence="10">7.2.2.11</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="14" type="ORF">J422_02749</name>
    </gene>
    <organism evidence="14 15">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="14 15" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="14 15">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>Ni(2+)(out) + ATP + H2O = Ni(2+)(in) + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:15557"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:49786"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="7.2.2.11"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="12">
            <dbReference type="Rhea" id="RHEA:15558"/>
        </physiologicalReaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">The complex is composed of two ATP-binding proteins (NikD and NikE), two transmembrane proteins (NikB and NikC) and a solute-binding protein (NikA).</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Peripheral membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="14">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="7.2.2.11" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96362.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590604.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ07"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00101"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.539"/>
    <dbReference type="OrthoDB" id="18209at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015413">
        <property type="term" value="F:ABC-type nickel transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015833">
        <property type="term" value="P:peptide transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd03257">
        <property type="entry name" value="ABC_NikE_OppD_transporters"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050388">
        <property type="entry name" value="ABC_Ni/Peptide_Import"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013563">
        <property type="entry name" value="Oligopep_ABC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01727">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43297:SF13">
        <property type="entry name" value="NICKEL ABC TRANSPORTER, ATP-BINDING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43297">
        <property type="entry name" value="OLIGOPEPTIDE TRANSPORT ATP-BINDING PROTEIN APPD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08352">
        <property type="entry name" value="oligo_HPY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="5" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="15" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6" id="KW-1278">Translocase</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="domain" description="ABC transporter" evidence="13">
        <location>
            <begin position="4"/>
            <end position="249"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004202"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022967"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00038669"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00039098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00044143"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048610"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="13">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="EMBL" id="ENN96362.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="15">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="314" mass="35700" checksum="20EB1A788DDAC3F4" modified="2013-06-26" version="1">MLDIRNLSVEFKLGKKSVKAVNNVSLKINENETVCLIGESGSGKSVLGLSILKLLPENVKISGEVYYKNKNLLSLPEKELKNIRGKIIAWIPQTSSALNPVITVGEQIAEPLIYHLKLDKKRAWRRVIDLLNFFSIIPAEKRAKQYPHQYSGGMKQRALVAMGISAEPEFIIADEPTKGLDIVKKFNVIEAFKKIKKKKLSQLIITHDLHLAEVIGDKISVMYCGQVLEVCNKNRFFNEPLHPYSKALLEALPDRGLKPIRGFPPSMVNPPSGCKFHPRCDYCLDICKRKEPPFFKVEDSLVRCWRYDNWKESN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ79</accession>
    <name>N6VZ79_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">tRNA (pseudouridine(54)-N(1))-methyltransferase</fullName>
            <ecNumber evidence="8">2.1.1.257</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">trmY</name>
        <name evidence="9" type="ORF">J422_02365</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="7 8">Specifically catalyzes the N1-methylation of pseudouridine at position 54 (Psi54) in tRNAs.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 8">
            <text>pseudouridine(54) in tRNA + S-adenosyl-L-methionine = N(1)-methylpseudouridine(54) in tRNA + S-adenosyl-L-homocysteine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:55292"/>
            <dbReference type="Rhea" id="RHEA-COMP:14140"/>
            <dbReference type="Rhea" id="RHEA-COMP:14141"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57856"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65314"/>
            <dbReference type="ChEBI" id="CHEBI:74890"/>
            <dbReference type="EC" id="2.1.1.257"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the methyltransferase superfamily. TrmY family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.1.257" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96432.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590345.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ79"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00354"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.465"/>
    <dbReference type="OrthoDB" id="27492at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd18087">
        <property type="entry name" value="TrmY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1280.10:FF:000041">
        <property type="entry name" value="tRNA (pseudouridine(54)-N(1))-methyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1280.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00587">
        <property type="entry name" value="tRNA_methyltr_TrmY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029028">
        <property type="entry name" value="Alpha/beta_knot_MTases"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007158">
        <property type="entry name" value="TrmY"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029026">
        <property type="entry name" value="tRNA_m1G_MTases_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002560">
        <property type="entry name" value="PRK02135.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40703">
        <property type="entry name" value="TRNA (PSEUDOURIDINE(54)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40703:SF1">
        <property type="entry name" value="TRNA (PSEUDOURIDINE(54)-N(1))-METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04013">
        <property type="entry name" value="Methyltrn_RNA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75217">
        <property type="entry name" value="alpha/beta knot"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 8" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 8" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="3 8" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 8" id="KW-0819">tRNA processing</keyword>
    <feature type="binding site" evidence="8">
        <location>
            <position position="136"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="178"/>
            <end position="183"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="188"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00051723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00055056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00587"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96432.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="203" mass="23493" checksum="599F865892846553" modified="2013-06-26" version="1">MREFIFKANKTITSSNINLKDLPGSCGRLDLVCRCVSDSLFLSHDIRRDVIFYSILYGPPNPPIAIKFVGNELKKVSPDERNIAIFIKKAIKKFEELSEEEKKYWNESTPGIYVRRIDFKDLVLEKINEGKNIYYLHKNGKDVYEVKIKNPVFILGDHIGLTKEDEEFLEDKAIKISLSPLELHANHCIVIIHNILDRLEYGI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ92</accession>
    <name>N6VZ92_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">DNA primase DnaG</fullName>
            <ecNumber evidence="9">2.7.7.101</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">dnaG</name>
        <name evidence="11" type="ORF">J422_02095</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">RNA polymerase that catalyzes the synthesis of short RNA molecules used as primers for DNA polymerase during DNA replication.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>ssDNA + n NTP = ssDNA/pppN(pN)n-1 hybrid + (n-1) diphosphate.</text>
            <dbReference type="EC" id="2.7.7.101"/>
        </reaction>
    </comment>
    <comment type="subunit">
        <text evidence="9">Forms a ternary complex with MCM helicase and DNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="9">Belongs to the archaeal DnaG primase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.101" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96442.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590219.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ92"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01168"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.412"/>
    <dbReference type="OrthoDB" id="8643at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000428">
        <property type="term" value="C:DNA-directed RNA polymerase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000178">
        <property type="term" value="C:exosome (RNase complex)"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:1990077">
        <property type="term" value="C:primosome complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003899">
        <property type="term" value="F:DNA-directed RNA polymerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008143">
        <property type="term" value="F:poly(A) binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006269">
        <property type="term" value="P:DNA replication, synthesis of primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01029">
        <property type="entry name" value="TOPRIM_primases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.1360.10:FF:000010">
        <property type="entry name" value="DNA primase DnaG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.1360.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00007">
        <property type="entry name" value="DNA_primase_DnaG_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050219">
        <property type="entry name" value="DnaG_primase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR020607">
        <property type="entry name" value="Primase_DnaG_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034154">
        <property type="entry name" value="TOPRIM_DnaG/twinkle"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006171">
        <property type="entry name" value="TOPRIM_dom"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003108">
        <property type="entry name" value="PRK04031.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30313">
        <property type="entry name" value="DNA PRIMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30313:SF2">
        <property type="entry name" value="DNA PRIMASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01751">
        <property type="entry name" value="Toprim"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00493">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56731">
        <property type="entry name" value="DNA primase core"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50880">
        <property type="entry name" value="TOPRIM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 9" id="KW-0235">DNA replication</keyword>
    <keyword evidence="1 9" id="KW-0240">DNA-directed RNA polymerase</keyword>
    <keyword evidence="7" id="KW-0460">Magnesium</keyword>
    <keyword evidence="6" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="4 9" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="2 9" id="KW-0639">Primosome</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="8 9" id="KW-0804">Transcription</keyword>
    <keyword evidence="3 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Toprim" evidence="10">
        <location>
            <begin position="168"/>
            <end position="242"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00007"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS50880"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96442.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="365" mass="40897" checksum="E51FD426B3421615" modified="2013-06-26" version="1">MDYGTTKYIIYAELIADGYVEKNDIIGAIFGQTEGLLGEEFDLRELQKSGRIGRIEVELTNINGKSIAKLTIPSSLDRVETSILAAALETVDRVGPCMASLKVVKIEDIRIKKREYIIDRAKEILKEMMSNVDINRIIEEVKESVRMSEIIEYGEDKLPAGPDIDKSDEIIIVEGKADVLNLLRYGIRNAIAVEGASVPKTIIELSKKKITTAFTDGDRGGELILKELLQSCDIDYIARAPLGREVEELTKKEIIKCLKSRIPVNSEVKSIEVESLSNNDYNNYANKLLSLKDDEVIIIINGEEKKIKINELSNLDNIDTLLIKTIDQKTLDNIYNKINNIICLNKKIVKKPSNVSIMSIRGLTL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZS2</accession>
    <name>N6VZS2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Chlorohydrolase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01580</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96572.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589975.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZS2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00443"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.311"/>
    <dbReference type="OrthoDB" id="42910at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016810">
        <property type="term" value="F:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01305">
        <property type="entry name" value="archeal_chlorohydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.40.10">
        <property type="entry name" value="Urease, subunit C, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006680">
        <property type="entry name" value="Amidohydro-rel"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011059">
        <property type="entry name" value="Metal-dep_hydrolase_composite"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032466">
        <property type="entry name" value="Metal_Hydrolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050287">
        <property type="entry name" value="MTA/SAH_deaminase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005552">
        <property type="entry name" value="PRK07213.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794">
        <property type="entry name" value="AMINOHYDROLASE SSNA-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43794:SF5">
        <property type="entry name" value="CHLOROHYDROLASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01979">
        <property type="entry name" value="Amidohydro_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51338">
        <property type="entry name" value="Composite domain of metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51556">
        <property type="entry name" value="Metallo-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Amidohydrolase-related" evidence="1">
        <location>
            <begin position="43"/>
            <end position="350"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01979"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96572.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="365" mass="41708" checksum="B72DFF5F840937A6" modified="2013-06-26" version="1">MIIRGKFLYGEDFTLREGNLVIEEGIIKGFTNEREDIKFNGLIIPSIINAHTHIADNCIKDIGINKDLDELVKPPNGLKHKYLKILDDNIIIKGIRLAIDEMVSLGIRYFCDFREGGLKGVKLLRKALNNRRIKAIILGRPINIDVSEIEKILFSADGIGLSGANEYNDEELKIIYNLTKKYNKIFSIHVAEHRGAVEYSIEKYGKTELKRLLELNIKPDFIIHGTHLSDEDIYLLKEKNIPTVLCVRANLYFNVGMPNIKKLMENEIMLGIGSDNFMANSPSIFREMEFIYKLYHIEPKEILKMATINNAKILKLENVGLIDEGYKADLTFIKPTNAILFSKNIVASLMRVEKGDIINIWWNNA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZS4</accession>
    <name>N6VZS4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KEOPS complex Pcc1-like subunit</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01605</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CTAG/PCC1 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000011">
        <property type="protein sequence ID" value="ENN96577.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589980.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000011.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZS4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00448"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.316"/>
    <dbReference type="OrthoDB" id="8982at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.310.50">
        <property type="entry name" value="Alpha-D-phosphohexomutase, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015419">
        <property type="entry name" value="CTAG/Pcc1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011470">
        <property type="entry name" value="PRK14887.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09341">
        <property type="entry name" value="Pcc1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007073"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96577.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="85" mass="10115" checksum="CF6F6B4DF8239F35" modified="2013-06-26" version="1">MNRFKLILTFDSEEEAKIIYNAIYLEHISSQIRSRAEMKLERNVIKIHVESPDISILKASIYSYLRWISAAQNIYRKVRECHSIE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6W020</accession>
    <name>N6W020_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_00971</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000005">
        <property type="protein sequence ID" value="ENN96697.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589862.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000005.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W020"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00581"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.191"/>
    <dbReference type="OrthoDB" id="53113at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032324">
        <property type="term" value="P:molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000385">
        <property type="entry name" value="MoaA_NifB_PqqE_Fe-S-bd_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787:SF3">
        <property type="entry name" value="ARYLSULFATASE REGULATORY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43787">
        <property type="entry name" value="FEMO COFACTOR BIOSYNTHESIS PROTEIN NIFB-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01305">
        <property type="entry name" value="MOAA_NIFB_PQQE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0408">Iron</keyword>
    <keyword evidence="6" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Elp3/MiaA/NifB-like radical SAM core" evidence="7">
        <location>
            <begin position="39"/>
            <end position="221"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001966"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="SMART" id="SM00729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96697.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="275" mass="32027" checksum="E7ADAAF1A4A2B025" modified="2013-06-26" version="1">MIIRNEICDKLENIVKNLKYVKHCIGCEGLDLNIKDPKHHPSVELTQKCNLNCFFCYSKLKKVKRGIYGNLEEAKALTISQYGEPLFDIEGVKRAILLGKKYNLRVDLQTNGVFLNEEILEEFKELGLDIVMISLSCFSKESYRKITGKDYFDKVKENIKIASKYFHTIIRSIYLPNFNEKELIEMAKYFDGIVDEIMIHHPIAYNNNLPKEYKIKDLLMLVDKIHLEVKKTNVSIKGCLLSQLKEMDGFILANINYNAISEVPEIKREYHPLPW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTK7</accession>
    <name>N6UTK7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06723</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000057">
        <property type="protein sequence ID" value="ENN95659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTK7"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="22"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="22" mass="2336" checksum="FA2E44BF26F58A2F" modified="2013-06-26" version="1" fragment="single">MRVGIVGCGAIGCFLVRKAKEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTL7</accession>
    <name>N6UTL7_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">UspA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06588</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the universal stress protein A family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000055">
        <property type="protein sequence ID" value="ENN95674.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593723.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000055.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTL7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00602"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1282"/>
    <dbReference type="OrthoDB" id="105697at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00293">
        <property type="entry name" value="USP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006015">
        <property type="entry name" value="Universal_stress_UspA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006016">
        <property type="entry name" value="UspA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268">
        <property type="entry name" value="STRESS RESPONSE PROTEIN NHAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46268:SF6">
        <property type="entry name" value="UNIVERSAL STRESS PROTEIN UP12"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00582">
        <property type="entry name" value="Usp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006276">
        <property type="entry name" value="UspA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01438">
        <property type="entry name" value="UNVRSLSTRESS"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="UspA" evidence="2">
        <location>
            <begin position="1"/>
            <end position="141"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008791"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00582"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95674.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="145" mass="15941" checksum="54B57BF4219A09EA" modified="2013-06-26" version="1">MYKKIVIPTDGSDVSMEAVKHGLMIAKKFEAEAYAIYVVDLSPFIGLPAEGSWELIREMLVEEGEEALRRAKEIAEEMDVDLKTEILEGVPAREIVDFAKKKEADLIVMGTTGKTGLDVILLGSVAQKVIKKSHCPVLVVKKSNP</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTR9</accession>
    <name>N6UTR9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">ATPase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06289</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95739.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593365.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTR9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00280"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1225"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd09878">
        <property type="entry name" value="PIN_VapC_VirB11L-ATPase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1010">
        <property type="entry name" value="5'-nuclease"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029060">
        <property type="entry name" value="PIN-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002716">
        <property type="entry name" value="PIN_dom"/>
    </dbReference>
    <dbReference type="SMART" id="SM00670">
        <property type="entry name" value="PINc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF88723">
        <property type="entry name" value="PIN domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PIN" evidence="1">
        <location>
            <begin position="10"/>
            <end position="128"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95739.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="280" mass="31786" checksum="F36943C9F21D5887" modified="2013-06-26" version="1">MKGEVVLKNNSAVLDTSIIITGVINKLLMNKKIKNLKSIIIPLCVISEIRNLANDKGKRKADGIKGFKEIENLLNICKDFGIDIKYDGRRPNPDEIKLARDGELDDLIIDVAENTSSTLLTCDRVQYLTAVARKIDSCLYSPKGDLIKKKFFYDYEDRIRNIAKELYGAFVGYENNLPIILMSEQTYKMGYSEGIYNKDGIRFKLTADEHIVEKIPTVLQYLPKPEAIKIAKENNLKFLGYKKGVPIFYNPNVEEKICILGGRGIFIKLTNKKSLENVKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6UTZ5</accession>
    <name>N6UTZ5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">4Fe-4S ferredoxin iron-sulfur binding domain protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_05793</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95824.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592823.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UTZ5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01247"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1129"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10549">
        <property type="entry name" value="MtMvhB_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF1">
        <property type="entry name" value="FERREDOXIN III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14697">
        <property type="entry name" value="Fer4_21"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12837">
        <property type="entry name" value="Fer4_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="6"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="40"/>
            <end position="69"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="71"/>
            <end position="100"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="126"/>
            <end position="155"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="156"/>
            <end position="185"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="193"/>
            <end position="220"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="5">
        <location>
            <begin position="222"/>
            <end position="251"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95824.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="254" mass="28811" checksum="7EF27BB90F4F4AF5" modified="2013-06-26" version="1">MSIRSYKVKVITLLEAEKSLEEMGIKEKEIANKIKPIKYEIICINGSKCIRCNLCYKACPVNAIEMAKIRKPASIIKEKCVKCEICVQTCPVNAIYAIEGIARVEDNHVKYKVYQLNIPHRKIRLKNYSLDKDKCIKCGLCAKYCPTNAIKVEIRKSIDVDLSLCMGCSACQEVCPKKAIKVENEIGEVIKTKEIEFNKDLCVGCFVCLENCPVNAIEQDLDKIKINKNKCIYCGKCEEVCPMDAIKLFRIHSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU14</accession>
    <name>N6UU14_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Haloacid dehalogenase superfamily protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_05608</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95844.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592685.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU14"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00272"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1094"/>
    <dbReference type="OrthoDB" id="26985at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043565">
        <property type="term" value="F:sequence-specific DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd14820">
        <property type="entry name" value="TRAX"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.2140">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002848">
        <property type="entry name" value="Translin_fam"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036081">
        <property type="entry name" value="Translin_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011159">
        <property type="entry name" value="PRK14562.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10741">
        <property type="entry name" value="TRANSLIN AND TRANSLIN ASSOCIATED PROTEIN X"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01997">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF74784">
        <property type="entry name" value="Translin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0460">Magnesium</keyword>
    <keyword evidence="1" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="binding site" evidence="1">
        <location>
            <position position="83"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="1">
        <location>
            <position position="120"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PIRSR" id="PIRSR602848-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95844.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="201" mass="24422" checksum="D8F117B637967674" modified="2013-06-26" version="1">MCKEYLIDYLKEKDSTREEMIKLAREITRDCAMLIREIHKTNEPRNIEEKLKNIAESIKKLNLLCKYPEFVSYLSTPQQEFIEAYSLYKIKYHNTIPKFSEFDFIKEENYILGLADVIGELRRELLEAMKEDNVEEVEKYFKFMEDIYDFLMSFDYYYVVDNLRRKQDIARGILERTHGDIVYFLENLKLRKKIEEIKVKF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6UU53</accession>
    <name>N6UU53_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aspartate carbamoyltransferase</fullName>
            <ecNumber evidence="7">2.1.3.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">Aspartate transcarbamylase</fullName>
            <shortName evidence="7">ATCase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7 11" type="primary">pyrB</name>
        <name evidence="11" type="ORF">J422_05429</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5 7">Catalyzes the condensation of carbamoyl phosphate and aspartate to form carbamoyl aspartate and inorganic phosphate, the committed step in the de novo pyrimidine nucleotide biosynthesis pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 7">
            <text>carbamoyl phosphate + L-aspartate = N-carbamoyl-L-aspartate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:20013"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
            <dbReference type="ChEBI" id="CHEBI:32814"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
            <dbReference type="EC" id="2.1.3.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="1 7">Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 2/3.</text>
    </comment>
    <comment type="subunit">
        <text evidence="7">Heterooligomer of catalytic and regulatory chains.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the aspartate/ornithine carbamoyltransferase superfamily. ATCase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.1.3.2" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000039">
        <property type="protein sequence ID" value="ENN95884.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592480.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UU53"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00839"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1060"/>
    <dbReference type="OrthoDB" id="7792at2157"/>
    <dbReference type="UniPathway" id="UPA00070">
        <property type="reaction ID" value="UER00116"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016597">
        <property type="term" value="F:amino acid binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004070">
        <property type="term" value="F:aspartate carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006207">
        <property type="term" value="P:'de novo' pyrimidine nucleobase biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0044205">
        <property type="term" value="P:'de novo' UMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000001">
        <property type="entry name" value="Aspartate carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.1370:FF:000002">
        <property type="entry name" value="Aspartate carbamoyltransferase 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.1370">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00001">
        <property type="entry name" value="Asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006132">
        <property type="entry name" value="Asp/Orn_carbamoyltranf_P-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006130">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036901">
        <property type="entry name" value="Asp/Orn_carbamoylTrfase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002082">
        <property type="entry name" value="Asp_carbamoyltransf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006131">
        <property type="entry name" value="Asp_carbamoyltransf_Asp/Orn-bd"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00670">
        <property type="entry name" value="asp_carb_tr"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002032">
        <property type="entry name" value="PRK00856.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753:SF6">
        <property type="entry name" value="ASPARTATE CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45753">
        <property type="entry name" value="ORNITHINE CARBAMOYLTRANSFERASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00185">
        <property type="entry name" value="OTCace"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02729">
        <property type="entry name" value="OTCace_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00100">
        <property type="entry name" value="AOTCASE"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00101">
        <property type="entry name" value="ATCASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53671">
        <property type="entry name" value="Aspartate/ornithine carbamoyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00097">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7" id="KW-0665">Pyrimidine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7 8" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase carbamoyl-P binding" evidence="10">
        <location>
            <begin position="2"/>
            <end position="143"/>
        </location>
    </feature>
    <feature type="domain" description="Aspartate/ornithine carbamoyltransferase Asp/Orn-binding" evidence="9">
        <location>
            <begin position="151"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="53"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="54"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="82"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="103"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="131"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="134"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="227"/>
        </location>
        <ligand>
            <name>L-aspartate</name>
            <dbReference type="ChEBI" id="CHEBI:29991"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="264"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="265"/>
        </location>
        <ligand>
            <name>carbamoyl phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:58228"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004852"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00008896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00043884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00048859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00001"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003634"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="Pfam" id="PF00185"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF02729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95884.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="304" mass="34789" checksum="A26BCBB654F58DBB" modified="2013-06-26" version="1">MKHLISMRDIGKDEIISILKESEKMEKLLNKKKPLKILEGKILATVFFEPSTRTRLSFETAMKRLGGDVITMTDVKTASTAKGESLIDTIRVLSGYSDIIVLRHPNEGAARLASEYSSVPIINAGDGSNQHPTQTLLDLYTINREIGKIDNIKIAFVGDLKYSRTVHSLVYALTLFDGVKLYFISPKDLEMPKDITEDLKAKGIEFYKSNCIQDLKNLDVNVLYVTRIQKERFPDPNEYERVKGSYRITKDLVEDKNFIIMHPLPRVDEIDYTVDMLEQAKYFKQSFYGIPVRMAILKKLLEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUD3</accession>
    <name>N6UUD3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">HTH crp-type domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04910</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95954.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592079.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUD3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00509"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.962"/>
    <dbReference type="OrthoDB" id="56502at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006355">
        <property type="term" value="P:regulation of DNA-templated transcription"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00092">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057161">
        <property type="entry name" value="DUF7839"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012318">
        <property type="entry name" value="HTH_CRP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012015">
        <property type="entry name" value="UCP_HTH_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43704">
        <property type="entry name" value="BSR5907 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43704:SF2">
        <property type="entry name" value="HTH CRP-TYPE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25211">
        <property type="entry name" value="DUF7839"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004955">
        <property type="entry name" value="HTH_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00419">
        <property type="entry name" value="HTH_CRP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="HTH crp-type" evidence="1">
        <location>
            <begin position="14"/>
            <end position="63"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00419"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95954.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="260" mass="29664" checksum="A70115DF4CEAA88E" modified="2013-06-26" version="1">MKKRNITELQILAEILRKQPYVKQKEIADNLDITVQAVSEHIRNLLKKGYIRSRGRGEYVITEKGIRRLKSWIKEFSSYLEEINSAIYRYKDIWPAIAEEDLNEGDEVYLHMKDGLLHATKKKVGEARAKILHGGKAGEDVAITEIRGIIDIPKSKVIIFKIPPETFGGSRNVDYEKIKEMLNKMENYVTATMGTVGYVVAKKLNLNPDIRFAVIEGIVNACNRGCNVIAFITGKMAERVIKRLNDEKINYIIIDVSRNV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUE5</accession>
    <name>N6UUE5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Large ribosomal subunit protein eL18</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">rpl18e</name>
        <name evidence="7" type="ORF">J422_04960</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 5">Belongs to the eukaryotic ribosomal protein eL18 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95964.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592099.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUE5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00498"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.972"/>
    <dbReference type="OrthoDB" id="11309at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006412">
        <property type="term" value="P:translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="FunFam" id="3.100.10.10:FF:000013">
        <property type="entry name" value="50S ribosomal protein L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.100.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00329">
        <property type="entry name" value="Ribosomal_eL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000039">
        <property type="entry name" value="Ribosomal_eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022947">
        <property type="entry name" value="Ribosomal_eL18_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021131">
        <property type="entry name" value="Ribosomal_uL15/eL18"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036227">
        <property type="entry name" value="Ribosomal_uL15/eL18_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001196">
        <property type="entry name" value="Ribosomal_uL15_CS"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003079">
        <property type="entry name" value="PRK04005.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934">
        <property type="entry name" value="60S RIBOSOMAL PROTEIN L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10934:SF2">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN EL18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17135">
        <property type="entry name" value="Ribosomal_L18"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52080">
        <property type="entry name" value="Ribosomal proteins L15p and L18e"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00475">
        <property type="entry name" value="RIBOSOMAL_L15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="2 5 7" id="KW-0689">Ribosomal protein</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL15/eL18" evidence="6">
        <location>
            <begin position="7"/>
            <end position="119"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006815"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00035218"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00329"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17135"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95964.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="120" mass="13549" checksum="4EEDB075C37D5BEE" modified="2013-06-26" version="1">MRRLMKTNPRLIRLIKTLRKASNKNNAKIWKVVAEKLAKPTRRRVEVNISKINRYAKENETIVVPGKVLGAGKLEKRVVVAAYAFSESAKRIIKEAGGEAITIEELLERNPKGSNVRIMA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="51" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUJ8</accession>
    <name>N6UUJ8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit PurQ</fullName>
            <shortName evidence="8">FGAM synthase</shortName>
            <ecNumber evidence="8">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Formylglycinamide ribonucleotide amidotransferase subunit I</fullName>
            <shortName evidence="8">FGAR amidotransferase I</shortName>
            <shortName evidence="8">FGAR-AT I</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Glutaminase PurQ</fullName>
            <ecNumber evidence="8">3.5.1.2</ecNumber>
        </alternativeName>
        <alternativeName>
            <fullName evidence="8">Phosphoribosylformylglycinamidine synthase subunit I</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">purQ</name>
        <name evidence="9" type="ORF">J422_04715</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="11 12">
            <text>L-glutamine + H2O = L-glutamate + NH4(+)</text>
            <dbReference type="Rhea" id="RHEA:15889"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="EC" id="3.5.1.2"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="8">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="8"/>
    <dbReference type="EC" id="3.5.1.2" evidence="8"/>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96014.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591985.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUJ8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00962"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.922"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd01740">
        <property type="entry name" value="GATase1_FGAR_AT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.880">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00421">
        <property type="entry name" value="PurQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029062">
        <property type="entry name" value="Class_I_gatase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010075">
        <property type="entry name" value="PRibForGlyAmidine_synth_PurQ"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01737">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002957">
        <property type="entry name" value="PRK03619.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47552:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURQ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13507">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF001586">
        <property type="entry name" value="FGAM_synth_I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01211">
        <property type="entry name" value="GATase_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52317">
        <property type="entry name" value="Class I glutamine amidotransferase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51273">
        <property type="entry name" value="GATASE_TYPE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 8" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 8" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 8" id="KW-0315">Glutamine amidotransferase</keyword>
    <keyword evidence="5 8" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="2 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 8" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <feature type="active site" description="Nucleophile" evidence="8">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="200"/>
        </location>
    </feature>
    <feature type="active site" evidence="8">
        <location>
            <position position="202"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022962"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00421"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96014.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="228" mass="25311" checksum="C0F76A27758BD704" modified="2013-06-26" version="1">MIAVIKFLGSNCDLDVCKAITLAGGKADLVFYTEKLDKYKAAVLPGGFSYGDYLRAGAIAARTGIINEIREMINKGYPVLGICNGAQIGLEAGFSKGVFTLNKNARFICRWVYIRVENNKTPFTQFYKKGEVLKIPIAHAEGRYYADDETLDYLYKKDMIVFKYCDETGEITEDANPNGSIDNIAGVCNEDYNCLLMMPHPERASEKILGSDDGLKVFKGMLHAIKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6UUM5</accession>
    <name>N6UUM5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04168</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96039.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591708.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UUM5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00686"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.816"/>
    <dbReference type="OrthoDB" id="65987at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96039.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="133" mass="15791" checksum="1D3DCEFA11544C03" modified="2013-06-26" version="1">MPKKVHDKLKIMEEILALGKKGEELTIYNITKKLNQNIKKLKCDEYHYVKEQTICNAINELKKLNCLEISIIEKTKIGKEKKIYKLTENSTKIFEVYKKYYSKIHKILKIMKSDEISDEELDIIIFILKRLAK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6UV76</accession>
    <name>N6UV76_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">FIST domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_03358</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96264.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590975.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UV76"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01057"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.658"/>
    <dbReference type="OrthoDB" id="140075at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR019494">
        <property type="entry name" value="FIST_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013702">
        <property type="entry name" value="FIST_domain_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40252">
        <property type="entry name" value="BLR0328 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40252:SF2">
        <property type="entry name" value="BLR0328 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08495">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF10442">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00897">
        <property type="entry name" value="FIST"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01204">
        <property type="entry name" value="FIST_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="FIST" evidence="1">
        <location>
            <begin position="25"/>
            <end position="240"/>
        </location>
    </feature>
    <feature type="domain" description="FIST C-domain" evidence="2">
        <location>
            <begin position="241"/>
            <end position="380"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00897"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM01204"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96264.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="404" mass="44130" checksum="DAC8FA977E09D359" modified="2013-06-26" version="1">MLKVGLGQSEDIDDAIEMVLQNVSKPNLTFVFSSSCFNPHEVYNRVKEKISGHIIGGTTAGEYTSLFKKPKNNTIAIMSLESKALKVGLGVGKDLVVNPELAGKNAAKESYKNIVRDPLLAHLIYLGLIRSKEIKNLNINPFVSITLIDGLSGLEEPAMRGILSILGRNIRIVGGSTGDDLKFNMTYQYADGVYNNAIINTIVAGLKMGTGMGHPYKPTDKGAVVTKARGRTIYELNNRPAAEVLKELLDVDSLTPEILSENPFGLRSSDIYGDYIIKSAMRENPDGSVSLYSEIYEGSYLSIMETDRETAIESFKKAIMMAIGDANIEKEDVGAIVIFNCILRHFLKEKLKINDVDIIKEVVGDVPVIGFNTYGEQGSTRGGAIGHYNQTSTILVISKKVISQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6UVW8</accession>
    <name>N6UVW8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">DNA helicase</fullName>
            <ecNumber evidence="1">3.6.4.12</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_02255</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.4.12" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96474.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590283.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UVW8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01199"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.443"/>
    <dbReference type="OrthoDB" id="129238at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003678">
        <property type="term" value="F:DNA helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="FunFam" id="2.20.28.10:FF:000003">
        <property type="entry name" value="DNA helicase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2" id="KW-0235">DNA replication</keyword>
    <keyword evidence="7" id="KW-0238">DNA-binding</keyword>
    <keyword evidence="5" id="KW-0347">Helicase</keyword>
    <keyword evidence="4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00012551"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022806"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023125"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96474.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="60" mass="6725" checksum="831D6B9B182E5110" modified="2013-06-26" version="1">MDMKVFICTKCNKLIEVPYGVPKPEKCPHCGAESIYIHRVDSGGRGLGRGMGRRCGRRFL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6UW12</accession>
    <name>N6UW12_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Transcriptional regulator TrmB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01825</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96514.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590120.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UW12"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00214"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.359"/>
    <dbReference type="OrthoDB" id="51378at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd00090">
        <property type="entry name" value="HTH_ARSR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011991">
        <property type="entry name" value="ArsR-like_HTH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051797">
        <property type="entry name" value="TrmB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002831">
        <property type="entry name" value="Tscrpt_reg_TrmB_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34293:SF1">
        <property type="entry name" value="HTH-TYPE TRANSCRIPTIONAL REGULATOR TRMBL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01978">
        <property type="entry name" value="TrmB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Transcription regulator TrmB N-terminal" evidence="1">
        <location>
            <begin position="20"/>
            <end position="93"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01978"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96514.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="128" mass="15066" checksum="F80F07B673F64A69" modified="2013-06-26" version="1">MSKILFKTPCTTWTFDSLMSCVFGIKSSDVRVYFDILKHKNSKITDIANRLNRDRSTIQRSVQNLMSAGLVKRRQINLKDGGYYYVYSAIPFEEVKKKIKETLDIWYNQMKAWVEEIEADDVINELRD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWB8</accession>
    <name>N6UWB8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cytidyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_01323</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000009">
        <property type="protein sequence ID" value="ENN96599.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589928.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000009.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWB8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00024"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.259"/>
    <dbReference type="OrthoDB" id="68916at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.620">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032266">
        <property type="entry name" value="HIGH_NTase1_ass"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008513">
        <property type="entry name" value="tRNA(Met)_cyd_acetate_ligase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37825">
        <property type="entry name" value="TRNA(MET) CYTIDINE ACETATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37825:SF1">
        <property type="entry name" value="TRNA(MET) CYTIDINE ACETATE LIGASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05636">
        <property type="entry name" value="HIGH_NTase1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16581">
        <property type="entry name" value="HIGH_NTase1_ass"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52374">
        <property type="entry name" value="Nucleotidylyl transferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Putative cytidyltransferase-related C-terminal region" evidence="1">
        <location>
            <begin position="144"/>
            <end position="346"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF16581"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96599.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="352" mass="40801" checksum="9AF794F0392E50FD" modified="2013-06-26" version="1">MYLHLQNFLNDRLKVIDDAEYKNKESFKRVKEIIEEIKDVEGKDKVVCDFTEYNPLHKGHKYALEEGKKYGIFVSVLPGPLERSGRGVPYLFDRYIRARMAIEAGADLVVEGPAMGILGSGQYMRCLIKMFYCLGADYIVRGYIPEPTIEKVIEKINKGYHIRVKPYKIICIESGEVLGEKLEIDNYVIASMSQTIYKLNEEEKLNFNPKFIFVRRLEGISGTKIREAVLKGRFDSVKDMLPESTLKILEDLKSVGLERFILRRFDDLILRTANYDELIKYLPERLAKHIENNRPYHSVNEIKAILPNGFSKHVKERVISILEARISKEIRKKYVSNYPAEIRILGISKRIS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWF6</accession>
    <name>N6UWF6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Anaerobic ribonucleoside-triphosphate reductase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_01131</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96654.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589894.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWF6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00816"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.221"/>
    <dbReference type="OrthoDB" id="139164at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0031250">
        <property type="term" value="C:anaerobic ribonucleoside-triphosphate reductase complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004748">
        <property type="term" value="F:ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008998">
        <property type="term" value="F:ribonucleoside-triphosphate reductase (thioredoxin) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009265">
        <property type="term" value="P:2'-deoxyribonucleotide biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006260">
        <property type="term" value="P:DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd01675">
        <property type="entry name" value="RNR_III"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005144">
        <property type="entry name" value="ATP-cone_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012833">
        <property type="entry name" value="NrdD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02487">
        <property type="entry name" value="NrdD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21075">
        <property type="entry name" value="ANAEROBIC RIBONUCLEOSIDE-TRIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR21075:SF0">
        <property type="entry name" value="ANAEROBIC RIBONUCLEOSIDE-TRIPHOSPHATE REDUCTASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03477">
        <property type="entry name" value="ATP-cone"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13597">
        <property type="entry name" value="NRDD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51161">
        <property type="entry name" value="ATP_CONE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2 3" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="1 3" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-cone" evidence="5">
        <location>
            <begin position="14"/>
            <end position="98"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="112"/>
            <end position="139"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00492"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51161"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96654.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="762" mass="88410" checksum="2E1FEDF0A2A7E5B3" modified="2013-06-26" version="1">MINSKELKDFVSSVFIIKKDGKRERFDINKLARSLINSGVNYKDIDFIVNEIQKKVYNGMTTDELKENIYKILKKIDRDVAENYYRGIILRVRTSNNQFEPFDKEKIVKALVREANADINIAKKIADDVERELKKMNVRYLTAPLIREIVNYKLIEYGLEELRHNHTRVGIPVYDITRLIKCGNRENANLLHNPESIHKWVADEAMKQYALLKLFPKHIADAHLKGDIHLHDLEYAATRPVCLQHDLRPFFKYGLKVDGTGLHTSVSKPAKHPEVAIQHAAKVLMAAQTNMAGGQSIDEFNIWLAPYVRGLTYEKIKQLMQMFIYELNQMYVARGGQVIFSSINLELEVPDFLKDKPAIKGGEVVGTYEEYEEEARMILEALLDVMLEGDALGKPFLFPNFIVKLRENAFKNENYGLLIKIHQLSAKFGIPYFINMFPEWQKINTNAMGCRTRLSADWTGDAEKDTLRTGNMQWYSINLPRIAYEAKGDDDKLFEILNDKLELVKEALLIKHKITLERLYKDKILPFLTQKFDGEDYYRYEHTTKSIGFVGLNEMVKYHLGEELHEKEALKFGVKVIEYIREYTNKLKDETGLRWSVTQTPAESTAGRFARLDYKYYKEETLSVVRGDLNDINSLYYTNSSHVRVDAPITLSEKILVEEKFHPLCNGGHILHVWNIERRADPDVLLDLTKKISKRDIGFWTYTKNLTICENCRKGYGGLLDKCPNCGSESLAKYSRITGYLQNINNWNPAKKQELKDRKWWI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWG1</accession>
    <name>N6UWG1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Transport system permease protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_01166</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the binding-protein-dependent transport system permease family. FecCD subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96659.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589899.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWG1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00811"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.228"/>
    <dbReference type="OrthoDB" id="27848at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0033214">
        <property type="term" value="P:siderophore-iron import into cell"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd06550">
        <property type="entry name" value="TM_ABC_iron-siderophores_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3470.10">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037294">
        <property type="entry name" value="ABC_BtuC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000522">
        <property type="entry name" value="ABC_transptr_permease_BtuC"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472:SF25">
        <property type="entry name" value="ABC TRANSPORTER PERMEASE PROTEIN MJ0876-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR30472">
        <property type="entry name" value="FERRIC ENTEROBACTIN TRANSPORT SYSTEM PERMEASE PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01032">
        <property type="entry name" value="FecCD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF81345">
        <property type="entry name" value="ABC transporter involved in vitamin B12 uptake, BtuC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 8" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 8" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 8" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="76"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="108"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="141"/>
            <end position="164"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="170"/>
            <end position="188"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="216"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="257"/>
            <end position="289"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="301"/>
            <end position="324"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="8">
        <location>
            <begin position="336"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007935"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96659.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="364" mass="40764" checksum="39AD5D0373903CC3" modified="2013-06-26" version="1">MRKILIPFLLISSLILFILSLYYSGTAESITTSDVNNFIIYKIKSGINYLFNKHVFDTYLSEKDRFKFELLEQYRFPPTIGAIFVGIILSASGLMLQTLFRNLLASPYTTGISSGVLLIAVLVIFNDSLSKLMISLFGGKYLVVAGWIGGIISMLMLLIIAYRVRESNGVLIVALLLTYFFSGIRAFYISQAEDIKVHEYYGYVIGNLKKITLNDLPIMAICTIIFLIALIFLVKPLNALLFGERYAKSFGLDIKKIRILILLTTCFAVGSIIPFVGLMAFVGIVSPYLARIIIKTSDHKYLLPTTMLLGVNLLLFCHILTVKYASFLNIKYAPPLPIGSLLDIIGGLLVIYLIFKEEKKIVIQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWP1</accession>
    <name>N6UWP1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Membrane protein-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00541</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96759.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589790.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWP1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00060"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.104"/>
    <dbReference type="OrthoDB" id="63940at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011314">
        <property type="entry name" value="Prd_NiFe_hyd_3_EhaB"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26645">
        <property type="entry name" value="EhaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019706">
        <property type="entry name" value="EhaB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="5"/>
            <end position="24"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="71"/>
            <end position="91"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="98"/>
            <end position="116"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96759.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="160" mass="17001" checksum="3402FDEB816CF7BC" modified="2013-06-26" version="1">MIEIIIATILCWLNFVIIDTIFGLPEKPGVLGAKVIGEKIEKIGGNLNGGYFMGNIVCSPDASAGTLLGSIMTYLMGFDGGLLAAFLVWIGNRLCHDPGYAGSIGALSITVIITLLKPFITPESFIVGSVLAIFTIQGLCHKEASKVLGNIYKAVIGWKR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="58" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWS5</accession>
    <name>N6UWS5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 9">Methionine aminopeptidase</fullName>
            <shortName evidence="8">MAP</shortName>
            <shortName evidence="8">MetAP</shortName>
            <ecNumber evidence="8 9">3.4.11.18</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Peptidase M</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="8" type="primary">map</name>
        <name evidence="11" type="ORF">J422_00035</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8 9">Removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 8 9">
            <text>Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.</text>
            <dbReference type="EC" id="3.4.11.18"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="8">
            <name>Co(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:48828"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="8">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
        <text evidence="8">Binds 2 divalent metal cations per subunit. Has a high-affinity and a low affinity metal-binding site. The true nature of the physiological cofactor is under debate. The enzyme is active with cobalt, zinc, manganese or divalent iron ions. Most likely, methionine aminopeptidases function as mononuclear Fe(2+)-metalloproteases under physiological conditions, and the catalytically relevant metal-binding site has been assigned to the histidine-containing high-affinity site.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="3">
            <name>Fe(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29033"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="8">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the peptidase M24A family. Methionine aminopeptidase archaeal type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.4.11.18" evidence="8 9"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96794.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589700.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWS5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01160"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.7"/>
    <dbReference type="OrthoDB" id="372008at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004239">
        <property type="term" value="F:initiator methionyl aminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070006">
        <property type="term" value="F:metalloaminopeptidase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006508">
        <property type="term" value="P:proteolysis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd01088">
        <property type="entry name" value="MetAP2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.230.10">
        <property type="entry name" value="Creatinase/methionine aminopeptidase superfamily"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.10">
        <property type="entry name" value="Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01975">
        <property type="entry name" value="MetAP_2_arc"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036005">
        <property type="entry name" value="Creatinase/aminopeptidase-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050247">
        <property type="entry name" value="Met_Aminopeptidase_Type2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028595">
        <property type="entry name" value="MetAP_archaeal"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000994">
        <property type="entry name" value="Pept_M24"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001714">
        <property type="entry name" value="Pept_M24_MAP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002468">
        <property type="entry name" value="Pept_M24A_MAP2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018349">
        <property type="entry name" value="Pept_M24A_MAP2_BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036388">
        <property type="entry name" value="WH-like_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00501">
        <property type="entry name" value="met_pdase_II"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45777:SF2">
        <property type="entry name" value="METHIONINE AMINOPEPTIDASE 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00557">
        <property type="entry name" value="Peptidase_M24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00599">
        <property type="entry name" value="MAPEPTIDASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55920">
        <property type="entry name" value="Creatinase/aminopeptidase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01202">
        <property type="entry name" value="MAP_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 8 9" id="KW-0031">Aminopeptidase</keyword>
    <keyword evidence="7 8 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 8 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 8 9" id="KW-0645">Protease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Peptidase M24" evidence="10">
        <location>
            <begin position="3"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="61"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="81"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="152"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="160"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="185"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>2</label>
            <note>catalytic</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="275"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
            <label>1</label>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000294"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00001954"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022438"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022670"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003653"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00557"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96794.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="291" mass="32849" checksum="C298763A1EBF9DCD" modified="2013-06-26" version="1">MLEKILKAGEIAKTVREEAKKMIKPGVKLLEVAEFVESRIRELGGEPAFPCNISINNIAAHYTPKINDPLYFKEDDVVKLDLGAHVDGYIADTAVTVDLSNSYRDLVKASEDALNTVIKEIEVPMNVGLMGKIIQEVIESYGYKPISNLSGHVMHKYELHTGISIPNVYEKTDRCIDVGDLVAIEPFATDGFGMVRDGDLGNIYKFLHKRPVRLQKAKKLLDIIEKKYPYLPFAERWIIKDEKERIYLKSLLTSSSIYGYPMLIEKNNGMVSQAEHTIYINEDGKVIITTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="53" xmlns="https://uniprot.org/uniprot">
    <accession>N6UWV8</accession>
    <name>N6UWV8_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="13 20">Inosine-5'-monophosphate dehydrogenase</fullName>
            <shortName evidence="13">IMP dehydrogenase</shortName>
            <shortName evidence="13">IMPD</shortName>
            <shortName evidence="13">IMPDH</shortName>
            <ecNumber evidence="13 20">1.1.1.205</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">guaB</name>
        <name evidence="22" type="ORF">J422_00240</name>
    </gene>
    <organism evidence="22 23">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="22 23" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="22 23">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="13">Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate-limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12 13 20">
            <text>IMP + NAD(+) + H2O = XMP + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:11708"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57464"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
            <dbReference type="EC" id="1.1.1.205"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 13">
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
        </cofactor>
    </comment>
    <comment type="activity regulation">
        <text evidence="13">Mycophenolic acid (MPA) is a non-competitive inhibitor that prevents formation of the closed enzyme conformation by binding to the same site as the amobile flap. In contrast, mizoribine monophosphate (MZP) is a competitive inhibitor that induces the closed conformation. MPA is a potent inhibitor of mammalian IMPDHs but a poor inhibitor of the bacterial enzymes. MZP is a more potent inhibitor of bacterial IMPDH.</text>
    </comment>
    <comment type="pathway">
        <text evidence="13 20">Purine metabolism; XMP biosynthesis via de novo pathway; XMP from IMP: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="3 13">Homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 13 19">Belongs to the IMPDH/GMPR family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">Lacks conserved residue(s) required for the propagation of feature annotation.</text>
    </comment>
    <comment type="caution">
        <text evidence="22">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.205" evidence="13 20"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96829.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589735.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UWV8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01121"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.48"/>
    <dbReference type="OrthoDB" id="21361at2157"/>
    <dbReference type="UniPathway" id="UPA00601">
        <property type="reaction ID" value="UER00295"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003938">
        <property type="term" value="F:IMP dehydrogenase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000166">
        <property type="term" value="F:nucleotide binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006177">
        <property type="term" value="P:GMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006183">
        <property type="term" value="P:GTP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd04601">
        <property type="entry name" value="CBS_pair_IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00381">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000003">
        <property type="entry name" value="GMP reductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01964">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000644">
        <property type="entry name" value="CBS_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046342">
        <property type="entry name" value="CBS_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005990">
        <property type="entry name" value="IMP_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015875">
        <property type="entry name" value="IMP_DH/GMP_Rdtase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001093">
        <property type="entry name" value="IMP_DH_GMPRt"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01302">
        <property type="entry name" value="IMP_dehydrog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911:SF111">
        <property type="entry name" value="INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11911">
        <property type="entry name" value="INOSINE-5-MONOPHOSPHATE DEHYDROGENASE RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00571">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00478">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000130">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00116">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM01240">
        <property type="entry name" value="IMPDH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54631">
        <property type="entry name" value="CBS-domain pair"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51412">
        <property type="entry name" value="Inosine monophosphate dehydrogenase (IMPDH)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51371">
        <property type="entry name" value="CBS"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00487">
        <property type="entry name" value="IMP_DH_GMP_RED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11 18" id="KW-0129">CBS domain</keyword>
    <keyword evidence="6 13 20" id="KW-0332">GMP biosynthesis</keyword>
    <keyword evidence="4 13 20" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10 13 16" id="KW-0520">NAD</keyword>
    <keyword evidence="9 13 19" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="8 13 17" id="KW-0630">Potassium</keyword>
    <keyword evidence="7 13 20" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="23" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5" id="KW-0677">Repeat</keyword>
    <feature type="domain" description="CBS" evidence="21">
        <location>
            <begin position="96"/>
            <end position="151"/>
        </location>
    </feature>
    <feature type="domain" description="CBS" evidence="21">
        <location>
            <begin position="155"/>
            <end position="211"/>
        </location>
    </feature>
    <feature type="active site" description="Thioimidate intermediate" evidence="13 14">
        <location>
            <position position="303"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="13 14">
        <location>
            <position position="399"/>
        </location>
    </feature>
    <feature type="binding site" evidence="16">
        <location>
            <begin position="246"/>
            <end position="248"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="246"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 16">
        <location>
            <begin position="296"/>
            <end position="298"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 17">
        <location>
            <position position="298"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 17">
        <location>
            <position position="300"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <position position="301"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="13 17">
        <location>
            <position position="303"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <begin position="336"/>
            <end position="338"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <begin position="359"/>
            <end position="360"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <begin position="383"/>
            <end position="387"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13 15">
        <location>
            <position position="417"/>
        </location>
        <ligand>
            <name>IMP</name>
            <dbReference type="ChEBI" id="CHEBI:58053"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="471"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="472"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="13">
        <location>
            <position position="473"/>
        </location>
        <ligand>
            <name>K(+)</name>
            <dbReference type="ChEBI" id="CHEBI:29103"/>
            <note>ligand shared between two tetrameric partners</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005502"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011881"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022958"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023122"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00048028"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01964"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="PIRSR" id="PIRSR000130-4"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00703"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="19">
        <source>
            <dbReference type="RuleBase" id="RU003927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="20">
        <source>
            <dbReference type="RuleBase" id="RU003928"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="21">
        <source>
            <dbReference type="PROSITE" id="PS51371"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="22">
        <source>
            <dbReference type="EMBL" id="ENN96829.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="23">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="490" mass="53033" checksum="373C769A4371DBFB" modified="2013-06-26" version="1">MFFKKLKEAEVAYTFDDVLLVPNASYVEPKETDVSTNLSGLKLNIPILSAAMDTVTEKEMAIALARLGGLGVIHRNMSIEEQVHQVLAVKKADEIVIKDVIVISPEETVKTAINLMETYSISGLPVVDGEKLVGIITHRDVKAIKDKDKKVKDVMTKDIVYAKENITEEEALDIMYSNRVERLPIVDDEMNLIGIVTLRDILKRRKYPQAARDKKGRLLVAAACGPHDFERAKALIEAEVDALVIDCAHAHNMRVVENIKKFKELCGDTVLIAGNIATKEAAEDLIAAGADVLKVGIGPGSICTTRIVAGVGVPQLTAIAKVADVAKNYEIPVIADGGIRYSGDIAKAIAAGADAVMLGSLLAGTDEAPGKLMVINGRKYKQYRGMGSLGAMMSGSGDRYFQTVDKSHMKHVKLVPEGVEGAVPYKGPVSEVIFQLLGGLRASLGYCGAKNLKELQEKGRFVIITQSGQRESHPHDILITNEAPNYPLGR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6UZY6</accession>
    <name>N6UZY6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">PDGLE domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_07002</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000067">
        <property type="protein sequence ID" value="ENN95593.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594701.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000067.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6UZY6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00577"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1356"/>
    <dbReference type="OrthoDB" id="88314at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025937">
        <property type="entry name" value="PDGLE_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13190">
        <property type="entry name" value="PDGLE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="5 6" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 6" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="4 6" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="6">
        <location>
            <begin position="84"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="domain" description="PDGLE" evidence="7">
        <location>
            <begin position="15"/>
            <end position="108"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004236"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF13190"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN95593.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="115" mass="12693" checksum="FF7889659B31DF09" modified="2013-06-26" version="1">MNWDDPLVKKFAYLIGAMIILCPLGILLVWNYGDAWGEWDPSEVAKEVGASKVAGLLKLSGVWQYAILPDYNIPGWDDPLHASIGYIISAIVGVALCLAFYYIIVKLVYSKPTTT</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6V002</accession>
    <name>N6V002_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="11">Ammonium transporter</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="11" type="ORF">J422_06822</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Involved in the uptake of ammonium/ammonia (NH(4)(+)/NH(3)). Transport is electrogenic.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ammonia transporter channel (TC 1.A.11.2) family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000063">
        <property type="protein sequence ID" value="ENN95613.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594234.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000063.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V002"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01083"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1323"/>
    <dbReference type="OrthoDB" id="10960at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008519">
        <property type="term" value="F:ammonium channel activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097272">
        <property type="term" value="P:ammonium homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3430.10">
        <property type="entry name" value="Ammonium transporter AmtB like domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029020">
        <property type="entry name" value="Ammonium/urea_transptr"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018047">
        <property type="entry name" value="Ammonium_transpt_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024041">
        <property type="entry name" value="NH4_transpt_AmtB-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002229">
        <property type="entry name" value="RhesusRHD"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11730:SF6">
        <property type="entry name" value="AMMONIUM TRANSPORTER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00909">
        <property type="entry name" value="Ammonium_transp"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00342">
        <property type="entry name" value="RHESUSRHD"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF111352">
        <property type="entry name" value="Ammonium transporter"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01219">
        <property type="entry name" value="AMMONIUM_TRANSP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-0924">Ammonia transport</keyword>
    <keyword evidence="6 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="3" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="28"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="40"/>
            <end position="61"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="81"/>
            <end position="101"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="108"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="146"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="186"/>
            <end position="205"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="217"/>
            <end position="237"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="249"/>
            <end position="278"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="298"/>
            <end position="320"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="327"/>
            <end position="349"/>
        </location>
    </feature>
    <feature type="domain" description="Ammonium transporter AmtB-like" evidence="10">
        <location>
            <begin position="8"/>
            <end position="373"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005887"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023177"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00045370"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00909"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95613.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="382" mass="40312" checksum="F9098D7ECF891222" modified="2013-06-26" version="1">MEGIDVFFFLWAASLIFFMKAGFVALEIGQFSHRNVAYHCVLKLLDLAAVFIGYLVIGYGISYGFENIVPLLFGNFNVELGAWFMKMVMFAAAAVTIITGGVAERIKILPYFIGALIVGSILYPIVEHLVWGGGFNALGIAFHDYAGSGAVHLFGGLVGLMAAWVLGPRAKKYINGIPQALPGHNIPLAVLGAFMLAFGWYGFNIGSAASVGDGLELTRVAVATTVALAGGIIGGAISSKNDPLYTANGMCAGLVAVCSGVDLYTPIGALIVGLLAGLQQPFTYKFIEEKLKIDDVCAIGPVHAMSGLIGVICAGIPFLLREPTTSFIGQVFGAIIISLIAIIGGWVLYKGLDIVIGLRVPKDVEEMGLDEGVLHVSAYTIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6V070</accession>
    <name>N6V070_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF447 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_06441</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95703.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593539.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V070"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01287"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1254"/>
    <dbReference type="OrthoDB" id="146030at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.110.10">
        <property type="entry name" value="Electron Transport, Fmn-binding Protein, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.290">
        <property type="entry name" value="Hypothetical membrane protein ta0354_69_121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR049288">
        <property type="entry name" value="DUF447_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007386">
        <property type="entry name" value="DUF447_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012349">
        <property type="entry name" value="Split_barrel_FMN-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016733">
        <property type="entry name" value="UCP018747"/>
    </dbReference>
    <dbReference type="Pfam" id="PF20766">
        <property type="entry name" value="DUF447_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04289">
        <property type="entry name" value="DUF447_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018747">
        <property type="entry name" value="UCP018747"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50475">
        <property type="entry name" value="FMN-binding split barrel"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF447" evidence="1">
        <location>
            <begin position="3"/>
            <end position="113"/>
        </location>
    </feature>
    <feature type="domain" description="DUF447" evidence="2">
        <location>
            <begin position="125"/>
            <end position="179"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF04289"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF20766"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95703.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="183" mass="21645" checksum="ED6E1CF9DE5CC8E1" modified="2013-06-26" version="1">MIYEAVVCTKNNKAPIGVSFKDNYAILRLFYGSHTYNNLLKENYFSVNIVDPIELVKALITDEDDYKYYKDIPYIDRAYLSIFYKVTERKFLVKRDIYGESKLMIIKGKEIGRICLNKKIKPYCRADGLLVEMAIIYSRLNHIKDDKKEEFKKDMEKYYGVIKKVGSEEHKKLAKILIENFKH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6V093</accession>
    <name>N6V093_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Beta-lactamase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06259</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000050">
        <property type="protein sequence ID" value="ENN95733.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593353.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000050.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V093"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00287"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1219"/>
    <dbReference type="OrthoDB" id="7773at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016740">
        <property type="term" value="F:transferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd07713">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041712">
        <property type="entry name" value="DHPS-like_MBL-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052926">
        <property type="entry name" value="Metallo-beta-lactamase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13754:SF18">
        <property type="entry name" value="7,8-DIHYDROPTERIN-6-METHYL-4-(BETA-D-RIBOFURANOSYL)-AMINOBENZENE-5'-PHOSPHATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13754">
        <property type="entry name" value="METALLO-BETA-LACTAMASE SUPERFAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="20"/>
            <end position="98"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00753"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95733.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="251" mass="29303" checksum="1F324044E54BB791" modified="2013-06-26" version="1">MEIKILVDNTAYKRFYAQHGFSVLVNNKKRVLFDAGQSEIIIKNLKLFNELENFDYFVLSHGHYDHSDGLKYLLDYVDKIIAHKDAFIDRFCDGRYIGIDKDLKDIIMKKCDLILIEEPYKIEKDIIVSGYVPREHYYELDNFECIKDGKRVKDYVNDDMFIIAKNILITGCAHSGIINVIEYGKKIMDIDGVLGGFHLINASKEYIDYVYNYLSKQDFWFMPMHCTGFNALKKLSDLKNFVYGHVGKVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0C9</accession>
    <name>N6V0C9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0237 protein J422_05948</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_05948</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0237 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95778.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593037.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0C9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00569"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1158"/>
    <dbReference type="OrthoDB" id="27277at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd04872">
        <property type="entry name" value="ACT_1ZPV"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.70.260:FF:000032">
        <property type="entry name" value="UPF0237 protein SP_0238"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01054">
        <property type="entry name" value="UPF0237"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR045865">
        <property type="entry name" value="ACT-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002912">
        <property type="entry name" value="ACT_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050990">
        <property type="entry name" value="UPF0237/GcvR_regulator"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022986">
        <property type="entry name" value="UPF0237_ACT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001220">
        <property type="entry name" value="PRK00194.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34875">
        <property type="entry name" value="UPF0237 PROTEIN MJ1558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34875:SF6">
        <property type="entry name" value="UPF0237 PROTEIN MJ1558"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13740">
        <property type="entry name" value="ACT_6"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55021">
        <property type="entry name" value="ACT-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51671">
        <property type="entry name" value="ACT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ACT" evidence="2">
        <location>
            <begin position="4"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01054"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51671"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN95778.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="89" mass="10050" checksum="1905EA44452482D8" modified="2013-06-26" version="1">MRVVISVIGKDRPGIVAGIANVLAKHNANILDISQTIMQDLFTMIMLVDISNIDVDFSKLKEELEKTGKELGVEVIVQHEDIFKYMHRI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0Q5</accession>
    <name>N6V0Q5_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Radical SAM protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="ORF">J422_05294</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95893.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592372.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0Q5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01328"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1033"/>
    <dbReference type="OrthoDB" id="30736at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003824">
        <property type="term" value="F:catalytic activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051536">
        <property type="term" value="F:iron-sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006783">
        <property type="term" value="P:heme biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd21123">
        <property type="entry name" value="SPASM_MftC-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023885">
        <property type="entry name" value="4Fe4S-binding_SPASM_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050377">
        <property type="entry name" value="Radical_SAM_PqqE_MftC-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR04085">
        <property type="entry name" value="rSAM_more_4Fe4S"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228:SF7">
        <property type="entry name" value="PQQA PEPTIDE CYCLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11228">
        <property type="entry name" value="RADICAL SAM DOMAIN PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01386">
        <property type="entry name" value="main_SPASM_domain-containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01067">
        <property type="entry name" value="SPASM/twitch_domain_containing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="2" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <feature type="domain" description="Radical SAM core" evidence="5">
        <location>
            <begin position="101"/>
            <end position="312"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN95893.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="449" mass="50882" checksum="C15BB55D62A4AD0E" modified="2013-06-26" version="1">MAITLLSLLVSNPISKKVIKKLIEKDGNVTKIEKLLEAYAYGYEVDDEFNIIYKIIKTGLKAFGENEEELKERLKDAYWRRGFVSVLRGLVDFGIRKPFVPGAPFLVVWDLTYACNLRCKHCYSTAGKPWKDELNTEEAIRVIDILADAGVTALAFSGGEPLIRKDFFTLTKYAAEKGLFVAVATNGTMITKDVARKMKECGVGYVQISLDGRKETHEKFRGINGIYEKVIEGILNAKEAGLITCISTTATKLNVHDIPAIMDIAEELGVEWFMLYNFIPVGRGSFEIDLSAEEKEELLKELWNRLKSSNVNFMSTAPYYARIAIQNNSNIIPTHFYNPKLEGKLKTLADFIGGCGCGRFYLAIKANGDIEPCVFFPLKLANIKDFRSCEDFLKFWRENKVLNDLRNRDNIEICGKCKYRYVCGGCRARAYAYYKDYMKPDIGCILVKK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6V0S4</accession>
    <name>N6V0S4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">CRISPR system Cms protein Csm4</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_05264</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CRISPR-associated Csm4 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000035">
        <property type="protein sequence ID" value="ENN95913.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592276.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000035.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V0S4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00740"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1028"/>
    <dbReference type="OrthoDB" id="62727at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051607">
        <property type="term" value="P:defense response to virus"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005510">
        <property type="entry name" value="Csm4"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040932">
        <property type="entry name" value="Csm4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005537">
        <property type="entry name" value="RAMP_III_fam"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01903">
        <property type="entry name" value="cas5_csm4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17953">
        <property type="entry name" value="Csm4_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03787">
        <property type="entry name" value="RAMPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0051">Antiviral defense</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3" id="KW-0694">RNA-binding</keyword>
    <feature type="domain" description="CRISPR type III-associated protein" evidence="5">
        <location>
            <begin position="13"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="Csm4 C-terminal" evidence="6">
        <location>
            <begin position="264"/>
            <end position="354"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005772"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00016109"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023118"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="Pfam" id="PF03787"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF17953"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95913.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="357" mass="41486" checksum="E43EB295FF9A6978" modified="2013-06-26" version="1">MKKLVILKPSVNSKYHFGSGDLEKSDIIFHSSNLFSAIVNNFVLLYGEDYKEEINKKVKELAENLRLSSLFIKIDDIFLLPKPEHPIFYLLKKEVYENIKPKDIKKIKFVTLKAYKEIVMEKLKENNCYIDSLFEDDLDRYFNKRVVNGIFATEEEVNKIKNKLKIKKLFEAYDEEKNTIDRICERPAEGQLYKVEFIKFDKNVELYFIIDYPEYLNKEIQASIKLIEDEGLGGKRSIGAGHFEKVEIKDIKKFKDFNELFNTQGELNILLGVGIPKEDEISNIKYYKLLELGGYIYSTTKPELVTKHRRSLMALSEGSVVKAFEGCVKDVKPKNSSENIHPVYTHGKPITLPLVEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1D8</accession>
    <name>N6V1D8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Oligosaccharide amylase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_04358</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the glycosyl hydrolase 15 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96073.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591776.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1D8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00651"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.854"/>
    <dbReference type="OrthoDB" id="36362at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004553">
        <property type="term" value="F:hydrolase activity, hydrolyzing O-glycosyl compounds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.50.10.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008928">
        <property type="entry name" value="6-hairpin_glycosidase_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012341">
        <property type="entry name" value="6hp_glycosidase-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011613">
        <property type="entry name" value="GH15-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616:SF0">
        <property type="entry name" value="GLUCAN 1,4-ALPHA-GLUCOSIDASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR31616">
        <property type="entry name" value="TREHALASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00723">
        <property type="entry name" value="Glyco_hydro_15"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48208">
        <property type="entry name" value="Six-hairpin glycosidases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="562"/>
            <end position="583"/>
        </location>
    </feature>
    <feature type="domain" description="GH15-like" evidence="3">
        <location>
            <begin position="252"/>
            <end position="535"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006188"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96073.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="592" mass="69108" checksum="B0F781876BCEB943" modified="2013-06-26" version="1">MIVGNNSFLCKIGDHGEIEYAFYPHVGYELHFFDSSLAIYDKEIMWIWDKEWSVYQKYIEDTNIFKTTLENENIIFVIKDLVPISHNVLIRRVFIKNKLPYNYNFKLFFYENLRIGEHPSENTVKFLDDCIVKFNGKYTFCISSDKKINSYQCGNRYSEKSAYKDIENGLLSENPESVGVLTDSAIEWDIDLKPHGKVAFNIYIFPHIGSNIEIIKNQLNIIKNLSSEIKNISLNYWKSSFDIKGYLFNEKYLKLAKRALMILTMLSDKNGGIIASPSIHPDYRYVWGRDGSYMAVALSIYGIKNIPWRFFHFMSKVQNLDGSWLQNYYTDGKPRLTALQIDQIGSVLWAMEVYYRTTGDREFVKKFWETIEKAGNFLYNASLSLMPCFDLWEEKYGVFSYTLGAMYGGLRAGCSLAKAIEEKKEDWKKALDKLKKDVDLLYLSDEERFVKSINPLNKEIDTSILGLSYPFGLVKVNDERMIKTAEAIEKAFKYKVGGIGRYPSDVYFGGNPWIITTLWLALYYRRLFITTNDRKYLEKSKKLFNWVINHIYLFPEQIHKELAIPVSAMPLGWSCAMLLFYLYKNDDIIVIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1F5</accession>
    <name>N6V1F5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF4352 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_04468</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000028">
        <property type="protein sequence ID" value="ENN96093.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591819.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000028.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1F5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00630"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.876"/>
    <dbReference type="OrthoDB" id="65533at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.60.40.1240">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029051">
        <property type="entry name" value="DUF4352"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029050">
        <property type="entry name" value="Immunoprotect_excell_Ig-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF11611">
        <property type="entry name" value="DUF4352"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0472">Membrane</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0732">Signal</keyword>
    <keyword evidence="2" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="2" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="2">
        <location>
            <begin position="6"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="domain" description="DUF4352" evidence="3">
        <location>
            <begin position="49"/>
            <end position="164"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022729"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF11611"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96093.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="195" mass="22522" checksum="BFF845F4BE390F38" modified="2013-06-26" version="1">MDIKTLIFSILILIIVMFSGMQILDFVKKSFYDGEFSINNSKEVIVKLKIGEFANNSKVNITVEDVMIVDTLKGNMGYRKIKNNETFVIVKIKAKNLQDKKPYQISNMDFTLLDENGKSYDAEMNVLDIKNALVIEAIPPKKSYEGYIIYKVPKDIKVIKIEYDFKNLNNLFDNYKAIWEVNVSDIPHLKYLSLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="60" xmlns="https://uniprot.org/uniprot">
    <accession>N6V1K4</accession>
    <name>N6V1K4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Polyamine aminopropyltransferase</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Putrescine aminopropyltransferase</fullName>
            <shortName evidence="6">PAPT</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Spermidine synthase</fullName>
            <shortName evidence="6">SPDS</shortName>
            <shortName evidence="6">SPDSY</shortName>
            <ecNumber evidence="6">2.5.1.16</ecNumber>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">speE</name>
        <name evidence="11" type="ORF">J422_03838</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Catalyzes the irreversible transfer of a propylamine group from the amino donor S-adenosylmethioninamine (decarboxy-AdoMet) to putrescine (1,4-diaminobutane) to yield spermidine.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="6 9">
            <text>S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12721"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:57443"/>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
            <dbReference type="ChEBI" id="CHEBI:326268"/>
            <dbReference type="EC" id="2.5.1.16"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Amine and polyamine biosynthesis; spermidine biosynthesis; spermidine from putrescine: step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Homodimer or homotetramer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6 8">Belongs to the spermidine/spermine synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.16" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96158.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591363.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V1K4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00399"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.752"/>
    <dbReference type="OrthoDB" id="10538at2157"/>
    <dbReference type="UniPathway" id="UPA00248">
        <property type="reaction ID" value="UER00314"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004766">
        <property type="term" value="F:spermidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008295">
        <property type="term" value="P:spermidine biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000056">
        <property type="entry name" value="Polyamine aminopropyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.140.10">
        <property type="entry name" value="Spermidine synthase, tetramerisation domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00198">
        <property type="entry name" value="Spermidine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030374">
        <property type="entry name" value="PABS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030373">
        <property type="entry name" value="PABS_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001045">
        <property type="entry name" value="Spermi_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035246">
        <property type="entry name" value="Spermidine_synt_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037163">
        <property type="entry name" value="Spermidine_synt_N_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002010">
        <property type="entry name" value="PRK00811.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00417">
        <property type="entry name" value="speE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558:SF11">
        <property type="entry name" value="SPERMIDINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11558">
        <property type="entry name" value="SPERMIDINE/SPERMINE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF17284">
        <property type="entry name" value="Spermine_synt_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01564">
        <property type="entry name" value="Spermine_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01330">
        <property type="entry name" value="PABS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51006">
        <property type="entry name" value="PABS_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="5 6 7" id="KW-0620">Polyamine biosynthesis</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 6 9" id="KW-0745">Spermidine biosynthesis</keyword>
    <keyword evidence="3 6 7" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="PABS" evidence="10">
        <location>
            <begin position="4"/>
            <end position="238"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6 7">
        <location>
            <position position="157"/>
        </location>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="33"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="64"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="88"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="108"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="139"/>
            <end position="140"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <begin position="157"/>
            <end position="160"/>
        </location>
        <ligand>
            <name>spermidine</name>
            <dbReference type="ChEBI" id="CHEBI:57834"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="6">
        <location>
            <position position="164"/>
        </location>
        <ligand>
            <name>S-methyl-5'-thioadenosine</name>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00007867"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023066"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023115"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00198"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00354"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="RuleBase" id="RU003836"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="PROSITE" id="PS51006"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96158.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="289" mass="33778" checksum="2CA63D19C20A984B" modified="2013-06-26" version="1">MKFNLWFTEYHNENISLSMRVKEVLYLEKSKYQEIAIIETYDFGRVLILDNTFQTTERDEFIYHELISHIPLFTHPNPKDVLVIGGGDGGTVREVVKHKKVERVDFVELDEKVIEACRKYLPNLSCEMDNEKVNIIIADGIKYVAETDKKYDVIIVDCPDPVGPAKGLFEKEFYKNVYKCLKDDGLMVQQSESPLYDLKLIENICKYLKESGFKIIMPYSYPMPSYPSGFWSFTLASKKYNPLDVDENKIREALKEIKTKYYDEEVHKAIFLSTPKYLKELVRNITKSL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6V267</accession>
    <name>N6V267_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="8">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_02824</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the UPF0104 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96373.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590626.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V267"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00114"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.554"/>
    <dbReference type="OrthoDB" id="15513at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022791">
        <property type="entry name" value="L-PG_synthase/AglD"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00374">
        <property type="entry name" value="flippase-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39087">
        <property type="entry name" value="UPF0104 MEMBRANE PROTEIN MJ1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR39087:SF2">
        <property type="entry name" value="UPF0104 MEMBRANE PROTEIN MJ1595"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03706">
        <property type="entry name" value="LPG_synthase_TM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="6 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="6"/>
            <end position="23"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="35"/>
            <end position="56"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="68"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="121"/>
            <end position="139"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="145"/>
            <end position="168"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="220"/>
            <end position="241"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="247"/>
            <end position="267"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="279"/>
            <end position="298"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="304"/>
            <end position="322"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011061"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96373.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="332" mass="38232" checksum="BD22207667FF07E3" modified="2013-06-26" version="1">MKKIVGFILSIVILLIILYIIGVKKVINVLLKVNLTFVFIAILMELVVILIMTLRWKYVLKVLDYSPSIKNLFLLVLLGQFINNITPSMRGGGEPFRAYYLYKLENIPKGVAFSSVLVERVLDTIIFLFMTITVIAHFITCGFEYTNYLIFSWIFIFITSFFLFYIILNKNLLIKMALKVNKFIEKFKKNKYNEDKILNSIEEFYSSLKMFKKSKKDGNVIIAIILSFLWYILDILKLWILFLAIPYYISIINVASVYLVTLLSGIFSPTPSGFGTSDIVMIGAFSLLNTNPSVATVITLLDRFISYILPTIFGYLASLYIYKKAGERYYGK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2E3</accession>
    <name>N6V2E3_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Phosphate uptake regulator, PhoU</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02370</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000015">
        <property type="protein sequence ID" value="ENN96433.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590346.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000015.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2E3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00355"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.466"/>
    <dbReference type="OrthoDB" id="7738at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030643">
        <property type="term" value="P:intracellular phosphate ion homeostasis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0045936">
        <property type="term" value="P:negative regulation of phosphate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.10.60">
        <property type="entry name" value="Homeodomain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.58.220">
        <property type="entry name" value="Phosphate transport system protein phou homolog 2, domain 2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR028366">
        <property type="entry name" value="PhoU"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038078">
        <property type="entry name" value="PhoU-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR026022">
        <property type="entry name" value="PhoU_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036390">
        <property type="entry name" value="WH_DNA-bd_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42930:SF3">
        <property type="entry name" value="PHOSPHATE-SPECIFIC TRANSPORT SYSTEM ACCESSORY PROTEIN PHOU"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13412">
        <property type="entry name" value="HTH_24"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01895">
        <property type="entry name" value="PhoU"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF109755">
        <property type="entry name" value="PhoU-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46785">
        <property type="entry name" value="Winged helix' DNA-binding domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="PhoU" evidence="1">
        <location>
            <begin position="83"/>
            <end position="166"/>
        </location>
    </feature>
    <feature type="domain" description="PhoU" evidence="1">
        <location>
            <begin position="181"/>
            <end position="258"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF01895"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96433.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="281" mass="32889" checksum="9F7DADEE11F2E260" modified="2013-06-26" version="1">MLRGKSATLAGIIKVIIEEEPETQDEIAEKLGISRRYVAKLLKPLIDEGVVKHPYVVDLKKLHKINLDFDEFYVMKNIIEILENMERVLLNELDSVYKALKENNKKIAEEVILKDYALNKMEEEVKMLLKISSIKYLPESYANAFIIIASNLERLGDYLANIAEEIVNGLKLDETIEDEVEKIFNILREMLKEAFEVVKTKKKETKIYRLEEELHRRLDKLLDLVLKNKKDDINFYIQFGMFLKDIERFGDRCVNIVDTALEAYHNIPKSSIPERLRRCRA</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2L6</accession>
    <name>N6V2L6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">ATP dependent DNA ligase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01750</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96503.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590108.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2L6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00201"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.344"/>
    <dbReference type="OrthoDB" id="14524at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016874">
        <property type="term" value="F:ligase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd07894">
        <property type="entry name" value="Adenylation_RNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.450.740">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.70">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2160">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.30">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR041596">
        <property type="entry name" value="Lig_Pab1020_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR021122">
        <property type="entry name" value="RNA_ligase_dom_REL/Rnl2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001072">
        <property type="entry name" value="RNA_ligase_Pab1020"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01209">
        <property type="entry name" value="RNA ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18330">
        <property type="entry name" value="Lig_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09414">
        <property type="entry name" value="RNA_ligase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01048">
        <property type="entry name" value="Y414FAMILY"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56091">
        <property type="entry name" value="DNA ligase/mRNA capping enzyme, catalytic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-0436">Ligase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNA ligase" evidence="1">
        <location>
            <begin position="88"/>
            <end position="247"/>
        </location>
    </feature>
    <feature type="domain" description="RNA ligase Pab1020 C-terminal" evidence="2">
        <location>
            <begin position="259"/>
            <end position="381"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09414"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF18330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96503.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="385" mass="45749" checksum="9F1A031EEC2247E2" modified="2013-06-26" version="1">MVKTYSLENIARKLNISIKDLNKAFKKKIIREDEFKNIRYLLFKKNFKNIEKGTVVILNEQLDIIRGYPKTYRALTLYPTIKEHFIDKVVIEEKLNGYNIRVAKIDDEILALTRSGYVCPFTTKKVKKYLKLEILDEYEHYMLCGEMIGKNNPYVPFYYEEAENDVENLAFFIFDIKERENNRSLPIKDRLKLCENYNLPHVRPIDIVDKDKAHEVIKDIIIELNNKRREGVVLKDPDMSVEPLKYTTHFINCQDLKEAFYFLFDLGLDFMFSRVIREGFMSYEFGECLEDREIRAIDIGKSILFPMVEAIEKVAKGERITEDFELIFDSENDFSEFLDFMRKMKMIIKIKNIERKEDRLKVLIGKVYNKTSDKIKAYLEGALWE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6V2T7</accession>
    <name>N6V2T7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Type 11 methyltransferase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02050</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96553.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590193.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V2T7"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.402"/>
    <dbReference type="OrthoDB" id="147504at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013216">
        <property type="entry name" value="Methyltransf_11"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591:SF24">
        <property type="entry name" value="2-METHOXY-6-POLYPRENYL-1,4-BENZOQUINOL METHYLASE, MITOCHONDRIAL"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43591">
        <property type="entry name" value="METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08241">
        <property type="entry name" value="Methyltransf_11"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase type 11" evidence="1">
        <location>
            <begin position="48"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF08241"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96553.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="250" mass="29128" checksum="0D081B250DA4D4A1" modified="2013-06-26" version="1">MEDVKECIKKYWDIRSESYDNSPGHSGLPEVWRSVLYDIFGDKKLRILDVGTGTGFLAILLAELGYEVVGIDLSERMLEKAKNKANKKGLDIEFMIADAENLPFNDEEFDAVINRHLLWTLPNPHRAINEWSRVVRKGGKVVVIDGRWKEETLEEKIRKAIGQVAIIIYERRNPWKNMDIYEKKGINKKLPFYGGSNPEDVINLFKRANLSNISVRDLKWIREKLNKNVPLLYRIAWKNKSYFLVEGYKI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6V326</accession>
    <name>N6V326_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">Aminotransferase</fullName>
            <ecNumber evidence="7">2.6.1.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_01101</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="cofactor">
        <cofactor evidence="1 7">
            <name>pyridoxal 5'-phosphate</name>
            <dbReference type="ChEBI" id="CHEBI:597326"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="3">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2 7">Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.6.1.-" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96648.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589888.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V326"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00822"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.215"/>
    <dbReference type="OrthoDB" id="372018at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030170">
        <property type="term" value="F:pyridoxal phosphate binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008483">
        <property type="term" value="F:transaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006520">
        <property type="term" value="P:amino acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00609">
        <property type="entry name" value="AAT_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.640.10:FF:000033">
        <property type="entry name" value="Aspartate aminotransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1150.10">
        <property type="entry name" value="Aspartate Aminotransferase, domain 1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.640.10">
        <property type="entry name" value="Type I PLP-dependent aspartate aminotransferase-like (Major domain)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004839">
        <property type="entry name" value="Aminotransferase_I/II_large"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050596">
        <property type="entry name" value="AspAT/PAT-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004838">
        <property type="entry name" value="NHTrfase_class1_PyrdxlP-BS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015424">
        <property type="entry name" value="PyrdxlP-dep_Trfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015421">
        <property type="entry name" value="PyrdxlP-dep_Trfase_major"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015422">
        <property type="entry name" value="PyrdxlP-dep_Trfase_small"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR46383:SF3">
        <property type="entry name" value="ASPARTATE AMINOTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00155">
        <property type="entry name" value="Aminotran_1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53383">
        <property type="entry name" value="PLP-dependent transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00105">
        <property type="entry name" value="AA_TRANSFER_CLASS_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 7 9" id="KW-0032">Aminotransferase</keyword>
    <keyword evidence="6" id="KW-0663">Pyridoxal phosphate</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 7 9" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Aminotransferase class I/classII large" evidence="8">
        <location>
            <begin position="25"/>
            <end position="368"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001933"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00007441"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00011738"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022576"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022898"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="RuleBase" id="RU000481"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="Pfam" id="PF00155"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96648.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="372" mass="42510" checksum="F95500CCBA4DB265" modified="2013-06-26" version="1">MIAKRCLNIKPSPIREVFNLANSNSINLGIGEPDFKTPEYIIEAAKRALDEGKTHYSPNNGIPELREAISEKLKKDYNIEVSCDNIMITCGASEGLMLAILSLVDRGDVAIVKEHTFVSYNSLINLAEGQVKAISLNEEFEFDVEELKEMIDKRTKLIIYNSPSNPTGKVYDKETVKAISEIAEDYNLIIISDEVYDKIVYEKKHYSPLQFTDRAILVNSFSKTYAMTGWRVGYLAVPEELNNELNLIDNFIKIHQYSFACATTFAQYGALEALKRDDFVNKMVKEFKRRRNLIYKGLKDIFNVYKPDGTFYIYPDVSEYGDSLEVAKKLIENNILCVPSIAFGNSNKYIRFSFATKYEDIEKAIEIIKELF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6V382</accession>
    <name>N6V382_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">4Fe-4S ferredoxin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00686</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96713.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589813.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V382"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00898"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.132"/>
    <dbReference type="OrthoDB" id="23833at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="CDD" id="cd10549">
        <property type="entry name" value="MtMvhB_like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="5"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.3270">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR043256">
        <property type="entry name" value="MvhB-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053559">
        <property type="entry name" value="Polyferredoxin"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052977">
        <property type="entry name" value="Polyferredoxin-like_ET"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF042909">
        <property type="entry name" value="FMH_DH_FwdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43193">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43193:SF2">
        <property type="entry name" value="POLYFERREDOXIN PROTEIN FWDF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005658">
        <property type="entry name" value="FwdF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="8"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="23"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="63"/>
            <end position="92"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="107"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="146"/>
            <end position="175"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="186"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="234"/>
            <end position="263"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="266"/>
            <end position="295"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="1">
        <location>
            <begin position="326"/>
            <end position="355"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96713.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="378" mass="41814" checksum="3D98558375828BE4" modified="2013-06-26" version="1">MIVQIKEYFEDGLIIKREGKQERELIWLDSKCVGCGICYDICPCSAIHLGPLGAIAKGLVDAPKLDIDKDACALCGLCAAACPFDAMDLKINGKSIKEDENYPKIKRSIKISQDKCVLCEQCEMVCPRDAITVDRELAERKNFVIGEININKDRCVLCGICADYCPADAIILDYIPPSPVCPKPVLDIKVDKDLCVYCKVCEFVCPHDAIEVICYKCPMIKRVKKAKLYKDITGETKVDKEKCVTCGWCALICPADAIEVEKPFEGEVIIDTNLCNACGACVAICPCSAIEFPESDKIKGKSEYPVSKEICETCGICLKDIPKKTEKIYVNQDLCVLCGACSKACPINAITIKRTKINFEREPEALAWKLAFKKLTTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3E7</accession>
    <name>N6V3E7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="10">Inner-membrane translocator</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="10" type="ORF">J422_00461</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Cell membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="8">Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96788.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589774.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3E7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00688"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.89"/>
    <dbReference type="OrthoDB" id="43815at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022857">
        <property type="term" value="F:transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006865">
        <property type="term" value="P:amino acid transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06582">
        <property type="entry name" value="TM_PBP1_LivH_like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001851">
        <property type="entry name" value="ABC_transp_permease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052157">
        <property type="entry name" value="BCAA_transport_permease"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795:SF449">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT PERMEASE PROTEIN LIVH-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11795">
        <property type="entry name" value="BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM PERMEASE PROTEIN LIVH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02653">
        <property type="entry name" value="BPD_transp_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0029">Amino-acid transport</keyword>
    <keyword evidence="3" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="7 9" id="KW-0472">Membrane</keyword>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 9" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 9" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="58"/>
            <end position="78"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="138"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="189"/>
            <end position="209"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="215"/>
            <end position="233"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="245"/>
            <end position="265"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="9">
        <location>
            <begin position="271"/>
            <end position="291"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022970"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00037998"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN96788.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="302" mass="32248" checksum="214A6F2CFA17CCE8" modified="2013-06-26" version="1">MIIEGAIIYANILALLALGLTLTYITTNVPNFAQGSFAVVCSYVALTLKELFKIHPYISLPILFIVGAFVGLITYLLLKPLIKRGASIEMLMIATLAIDVIIFGTLGAYSEILSPIINSTAAKFIFSNIDFSFFGFKGVFLVSTIVVVLLLTLLYIMLYKTKFGVALRASMENPSLAQAMGIDVEKTRLFSWLISGGLAGIAGGLLPFVEEIVPGTGGFIIISIFAASIVGGLRHISGAILGGYIIGLSETLVTYGLSLILGAGFLVYGKVISLIIMIATLLLAPEGITGIDWKKVRKRLEG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6V3J2</accession>
    <name>N6V3J2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TIGR00304 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00235</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96828.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589734.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6V3J2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01122"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.47"/>
    <dbReference type="OrthoDB" id="137740at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002849">
        <property type="entry name" value="DUF131"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00304">
        <property type="entry name" value="TIGR00304 family membrane protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01998">
        <property type="entry name" value="DUF131"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="6"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="56"/>
            <end position="78"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96828.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="82" mass="9371" checksum="4D8AB7AA4BA78350" modified="2013-06-26" version="1">MRALIFLGILLIFIGFFLVTLGMILPDKEKKEENVEYAGIIMIGPIPIVFGNSKNLIIFSILITILMIIWILMFLLSFRVVK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP12</accession>
    <name>N6VP12_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Methyl-accepting chemotaxis sensory transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_07092</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="2">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000074">
        <property type="protein sequence ID" value="ENN95570.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP12"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01218"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1372"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004888">
        <property type="term" value="F:transmembrane signaling receptor activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006935">
        <property type="term" value="P:chemotaxis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd11386">
        <property type="entry name" value="MCP_signal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd00158">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.250.10">
        <property type="entry name" value="Rhodanese-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004090">
        <property type="entry name" value="Chemotax_Me-accpt_rcpt"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004089">
        <property type="entry name" value="MCPsignal_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001763">
        <property type="entry name" value="Rhodanese-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036873">
        <property type="entry name" value="Rhodanese-like_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00015">
        <property type="entry name" value="MCPsignal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00581">
        <property type="entry name" value="Rhodanese"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00260">
        <property type="entry name" value="CHEMTRNSDUCR"/>
    </dbReference>
    <dbReference type="SMART" id="SM00283">
        <property type="entry name" value="MA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00450">
        <property type="entry name" value="RHOD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52821">
        <property type="entry name" value="Rhodanese/Cell cycle control phosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50111">
        <property type="entry name" value="CHEMOTAXIS_TRANSDUC_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50206">
        <property type="entry name" value="RHODANESE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="5" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 3" id="KW-0807">Transducer</keyword>
    <keyword evidence="5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="25"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="31"/>
            <end position="52"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="108"/>
            <end position="158"/>
        </location>
    </feature>
    <feature type="domain" description="Methyl-accepting transducer" evidence="6">
        <location>
            <begin position="177"/>
            <end position="434"/>
        </location>
    </feature>
    <feature type="domain" description="Rhodanese" evidence="7">
        <location>
            <begin position="494"/>
            <end position="563"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="89"/>
            <end position="116"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="143"/>
            <end position="177"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="4">
        <location>
            <begin position="367"/>
            <end position="457"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023224"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00284"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS50111"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50206"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95570.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="563" mass="64021" checksum="7776BEA83E0C9128" modified="2013-06-26" version="1">MKKLREYDYRILIIVLLFGLLIYFLSKINDLLAILSVFIFSFVSLLCIRLFLLRDAEKIVKLIDIFEKERKIIDIKVRNKTCLILHKRVVELLNNIIKKEEALKEERQEISKTVKELLYAIKKLEEGDFSVRLDENRKRNLLQKTFNRALDKLAEVLENIRKEVLELNHQIVILKDETKKAKDSIDQIVDASQQVAEAATSQSSKLQEVASDVEETDKLAIKTENLSKEGVEIAIRASEKADEGMVKVENAVNSMKKISDVIDDLSKSIEELGEKSKKITEITTLIKDIAKQTGLLALNASIEAARAGEAGRGFTVVASEIKGLAEEIGKSVDDVNKTIEEIFEQIEKTVNLSNIGKKEVDSGVNVVNEVNDKFLEIKNTIDETKEKIEEIKESAGKTIRNMEKALKEVQEVASISEEFAATAEELSANAEEEKKALKEIEEVIIKLENVSRSVRDNLMRIKTTRNISVDEAKELINNKDYLFIYVVPRDKYLGKIPNSIYIPINELKNNLDKIPKDKKIVVYCNMGFTSPSAYWLLKKEGYDVYNMEGGIKAWKEKGYPIES</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="16" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP55</accession>
    <name>N6VP55_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06817</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000062">
        <property type="protein sequence ID" value="ENN95635.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP55"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95635.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="33" mass="4033" checksum="2FEC527D62ED1D84" modified="2013-06-26" version="1" fragment="single">NIKKILKLEEELKKKLKHFHSAYDEFYEKIIVG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP74</accession>
    <name>N6VP74_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">NfeD-like C-terminal domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="7" type="ORF">J422_06633</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000056">
        <property type="protein sequence ID" value="ENN95665.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593808.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000056.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP74"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00366"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1291"/>
    <dbReference type="OrthoDB" id="60430at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052165">
        <property type="entry name" value="Membrane_assoc_protease"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002810">
        <property type="entry name" value="NfeD-like_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33507:SF7">
        <property type="entry name" value="HYPOTHETICAL MEMBRANE PROTEIN, CONSERVED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33507">
        <property type="entry name" value="INNER MEMBRANE PROTEIN YBBJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01957">
        <property type="entry name" value="NfeD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF141322">
        <property type="entry name" value="NfeD domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4 5" id="KW-0472">Membrane</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 5" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 5" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="7"/>
            <end position="40"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="5">
        <location>
            <begin position="46"/>
            <end position="67"/>
        </location>
    </feature>
    <feature type="domain" description="NfeD-like C-terminal" evidence="6">
        <location>
            <begin position="78"/>
            <end position="134"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01957"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN95665.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="137" mass="15184" checksum="58771696CD4C0AAF" modified="2013-06-26" version="1">MLGYFYIILGVIIVAIESIIPGLYFPAIGIALIVYGILLILFPNPIFDVISAILAGVITAIILYYFVYSVKVNVKVGVEGYVGKRGRAVEDFKEGYGRVEIENQTWLAKSEDEIKKGDKIEVIGYEGVHLIVKKVTK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VP97</accession>
    <name>N6VP97_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">KEOPS complex Cgi121-like protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06391</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the CGI121/TPRKB family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95695.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593524.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VP97"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01296"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1244"/>
    <dbReference type="OrthoDB" id="69587at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.2380.10">
        <property type="entry name" value="CGI121/TPRKB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013926">
        <property type="entry name" value="CGI121/TPRKB"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036504">
        <property type="entry name" value="CGI121/TPRKB_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF011465">
        <property type="entry name" value="PRK14886.1-1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08617">
        <property type="entry name" value="CGI-121"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF143870">
        <property type="entry name" value="PF0523-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005546"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95695.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="145" mass="16511" checksum="1495DAEA3B164EB7" modified="2013-06-26" version="1">MIIRGIRGAKIKEDIFNIKGFQILDADKIATKKHILHAINQAKTKKMITKDFWMEILVRASGQRQISEAIKIMGAKDGNVCLVCEDEEIFNKIYNIIGGEIDDSVLDLNEKKEKIIRNIFKIRGFGNLVERVLEKIALIELKGSK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPA5</accession>
    <name>N6VPA5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="8 10">NH(3)-dependent NAD(+) synthetase</fullName>
            <ecNumber evidence="8 10">6.3.1.5</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="primary">nadE</name>
        <name evidence="12" type="ORF">J422_06451</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="8">Catalyzes the ATP-dependent amidation of deamido-NAD to form NAD. Uses ammonia as a nitrogen source.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>deamido-NAD(+) + NH4(+) + ATP = AMP + diphosphate + NAD(+) + H(+)</text>
            <dbReference type="Rhea" id="RHEA:21188"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="6.3.1.5"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="8">Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1.</text>
    </comment>
    <comment type="subunit">
        <text evidence="8">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 8 9">Belongs to the NAD synthetase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.1.5" evidence="8 10"/>
    <dbReference type="EMBL" id="APMM01000052">
        <property type="protein sequence ID" value="ENN95705.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593544.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000052.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPA5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01285"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1256"/>
    <dbReference type="OrthoDB" id="39312at2157"/>
    <dbReference type="UniPathway" id="UPA00253">
        <property type="reaction ID" value="UER00333"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004359">
        <property type="term" value="F:glutaminase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003952">
        <property type="term" value="F:NAD+ synthase (glutamine-hydrolyzing) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008795">
        <property type="term" value="F:NAD+ synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009435">
        <property type="term" value="P:NAD+ biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00553">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.620:FF:000106">
        <property type="entry name" value="Glutamine-dependent NAD(+) synthetase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.620">
        <property type="entry name" value="HUPs"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00193">
        <property type="entry name" value="NadE_ammonia_dep"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022310">
        <property type="entry name" value="NAD/GMP_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003694">
        <property type="entry name" value="NAD_synthase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022926">
        <property type="entry name" value="NH(3)-dep_NAD(+)_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014729">
        <property type="entry name" value="Rossmann-like_a/b/a_fold"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00552">
        <property type="entry name" value="nadE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF010587">
        <property type="entry name" value="PRK13980.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090:SF9">
        <property type="entry name" value="GLUTAMINE-DEPENDENT NAD(+) SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23090">
        <property type="entry name" value="NH 3 /GLUTAMINE-DEPENDENT NAD + SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02540">
        <property type="entry name" value="NAD_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52402">
        <property type="entry name" value="Adenine nucleotide alpha hydrolases-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8 9" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="2 8 9" id="KW-0436">Ligase</keyword>
    <keyword evidence="6 8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="3 8" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="7 8 9" id="KW-0520">NAD</keyword>
    <keyword evidence="4 8 9" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="NAD/GMP synthase" evidence="11">
        <location>
            <begin position="2"/>
            <end position="239"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="24"/>
            <end position="31"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="30"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="107"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="127"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="132"/>
        </location>
        <ligand>
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <position position="140"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="147"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="156"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="178"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" description="in other chain" evidence="8">
        <location>
            <begin position="237"/>
            <end position="238"/>
        </location>
        <ligand>
            <name>deamido-NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:58437"/>
            <note>ligand shared between two neighboring subunits</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005859"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00193"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003811"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="RuleBase" id="RU003812"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02540"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95705.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="249" mass="28180" checksum="EE7FC593C2514D54" modified="2013-06-26" version="1">MIEKITKFIRDEVEKAKANGVVIGLSGGIDSTVTAFLCVKALGKDKVLGLIMPEKMSKIEDIEDAKMVAELLGIKYKIIDITDILKAFGVYSPVKEFEFADANLKPRVRMCLLYYYANKYNLLVVGTGNKSEWLTGYFTKYGDGAADIYPILDLYKTDVRNLAKELKVPEKIIMKTPSAGLWEGQTDEEELGISYDLLDKILILYEKGKNAEEISRELNIDIKKVEHVFNLIKKSEHKRAGVKYPKVRE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPL2</accession>
    <name>N6VPL2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Flagella accessory C family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_05858</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Archaeal flagellum</location>
        </subcellularLocation>
    </comment>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592844.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPL2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01235"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1142"/>
    <dbReference type="OrthoDB" id="121879at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097589">
        <property type="term" value="C:archaeal-type flagellum"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.5.490">
        <property type="entry name" value="Single helix bin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009205">
        <property type="entry name" value="FlaC_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052494">
        <property type="entry name" value="Flagella_assembly_related"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40698:SF2">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN C-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40698">
        <property type="entry name" value="FLAGELLA-RELATED PROTEIN E-RELATED-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05377">
        <property type="entry name" value="FlaC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF018660">
        <property type="entry name" value="Archl_flaC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF57997">
        <property type="entry name" value="Tropomyosin"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0974">Archaeal flagellum</keyword>
    <keyword evidence="4" id="KW-0966">Cell projection</keyword>
    <keyword evidence="4" id="KW-0969">Cilium</keyword>
    <keyword evidence="3" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4" id="KW-0282">Flagellum</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="4"/>
            <end position="45"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="3">
        <location>
            <begin position="70"/>
            <end position="97"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004618"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022440"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="141" mass="16224" checksum="5BB3459ECF053A35" modified="2013-06-26" version="1">METSENLMAKVSDLESRLPRLESSINALRKENEMLRIEIEKINESLQDIMSLYEVVSNQINPFIGVSKITATSLEKLERLEADYKKLKKTLEELTNDLVVLGSLYLRQLNINLEDIIKDVIEEELVKAMSGEDEHDTKDNK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6VPL5</accession>
    <name>N6VPL5_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Large ribosomal subunit protein uL2</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="10" type="primary">rpl2p</name>
        <name evidence="6" type="synonym">rpl2</name>
        <name evidence="10" type="ORF">J422_05588</name>
    </gene>
    <organism evidence="10 11">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="10 11" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="10 11">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity; this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the 50S ribosomal subunit. Forms a bridge to the 30S subunit in the 70S ribosome.</text>
    </comment>
    <comment type="similarity">
        <text evidence="1 6">Belongs to the universal ribosomal protein uL2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="10">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000043">
        <property type="protein sequence ID" value="ENN95840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592678.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000043.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VPL5"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00276"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1090"/>
    <dbReference type="OrthoDB" id="5987at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0022625">
        <property type="term" value="C:cytosolic large ribosomal subunit"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019843">
        <property type="term" value="F:rRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003735">
        <property type="term" value="F:structural constituent of ribosome"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002181">
        <property type="term" value="P:cytoplasmic translation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="FunFam" id="2.40.50.140:FF:000020">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="4.10.950.10:FF:000002">
        <property type="entry name" value="60S ribosomal protein L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.30:FF:000006">
        <property type="entry name" value="60S ribosomal protein L8"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.50.140">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="4.10.950.10">
        <property type="entry name" value="Ribosomal protein L2, domain 3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01320_A">
        <property type="entry name" value="Ribosomal_uL2_A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012340">
        <property type="entry name" value="NA-bd_OB-fold"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014722">
        <property type="entry name" value="Rib_uL2_dom2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002171">
        <property type="entry name" value="Ribosomal_uL2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023672">
        <property type="entry name" value="Ribosomal_uL2_arc_euk"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022669">
        <property type="entry name" value="Ribosomal_uL2_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022671">
        <property type="entry name" value="Ribosomal_uL2_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR014726">
        <property type="entry name" value="Ribosomal_uL2_dom3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022666">
        <property type="entry name" value="Ribosomal_uL2_RNA-bd_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008991">
        <property type="entry name" value="Translation_prot_SH3-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF007180">
        <property type="entry name" value="PRK09612.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691:SF16">
        <property type="entry name" value="LARGE RIBOSOMAL SUBUNIT PROTEIN UL2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR13691">
        <property type="entry name" value="RIBOSOMAL PROTEIN L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03947">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00181">
        <property type="entry name" value="Ribosomal_L2_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF002158">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01383">
        <property type="entry name" value="Ribosomal_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01382">
        <property type="entry name" value="Ribosomal_L2_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50249">
        <property type="entry name" value="Nucleic acid-binding proteins"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50104">
        <property type="entry name" value="Translation proteins SH3-like domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00467">
        <property type="entry name" value="RIBOSOMAL_L2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 6" id="KW-0687">Ribonucleoprotein</keyword>
    <keyword evidence="4 6 10" id="KW-0689">Ribosomal protein</keyword>
    <keyword evidence="3 6" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="2 6" id="KW-0699">rRNA-binding</keyword>
    <feature type="domain" description="Large ribosomal subunit protein uL2 RNA-binding" evidence="9">
        <location>
            <begin position="11"/>
            <end position="86"/>
        </location>
    </feature>
    <feature type="domain" description="Large ribosomal subunit protein uL2 C-terminal" evidence="8">
        <location>
            <begin position="92"/>
            <end position="227"/>
        </location>
    </feature>
    <feature type="region of interest" description="Disordered" evidence="7">
        <location>
            <begin position="203"/>
            <end position="227"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005636"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022730"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022980"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01320"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="MobiDB-lite"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="SMART" id="SM01382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM01383"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="EMBL" id="ENN95840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="243" mass="26544" checksum="BE5E1525AE89B8B4" modified="2013-06-26" version="1">MGKRLVQQRRGKGGSVYRCPSHKRRGAAKYRKYDDLEKNGKVVGKIIDILHDPGRSAPVAEVEFETGEKKIMLVPEGMKVGDIIECGVSADIKPGNVLPLYKIPEGTPIFNIETIPGDGGKLVRAGGCYAHVLTHDHENEKTYVKLPSGKIRVFDSKCRATIGVVAGGGRLEKPLVKAGKAYWKYKATGVKWPRVRGVAMNAVDHPFGGGRHQHTGKPTTVSRRMPPGRKVGHIAARRTGVRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="32" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQ67</accession>
    <name>N6VQ67_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Cobyrinic acid ac-diamide synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_04770</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000030">
        <property type="protein sequence ID" value="ENN96025.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592005.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000030.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQ67"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00973"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.933"/>
    <dbReference type="OrthoDB" id="36110at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009898">
        <property type="term" value="C:cytoplasmic side of plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051782">
        <property type="term" value="P:negative regulation of cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR025669">
        <property type="entry name" value="AAA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050625">
        <property type="entry name" value="ParA/MinD_ATPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384:SF10">
        <property type="entry name" value="ATPASE INVOLVED IN CHROMOSOME PARTITIONING, PARA_MIND FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43384">
        <property type="entry name" value="SEPTUM SITE-DETERMINING PROTEIN MIND HOMOLOG, CHLOROPLASTIC-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13614">
        <property type="entry name" value="AAA_31"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA" evidence="1">
        <location>
            <begin position="1"/>
            <end position="143"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF13614"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96025.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="255" mass="28548" checksum="87CA055E770F9466" modified="2013-06-26" version="1">MKVVTFAIAKGGTGKTIITANVSAALGKKGKRVLIIDNDIGSRSLSYLFNISPERYLIDIINKKSIKDAIIKTDVENIDILPMGKNLMDYLKLDIEDLNKFNEIKDYDYIFIDSPATSTGVETYISLGFSNYFIPVIDYTSLAPSLQGAINTLVIGKNYLESEPVGFIINKAEEVPESVKKDLEKILGVKCIAIINKNSFIEDSYEKKKILYLTGEDERFNKIIDNIVNFLEELEDKSEINIPSVIAKIKKATLF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="19" xmlns="https://uniprot.org/uniprot">
    <accession>N6VQX8</accession>
    <name>N6VQX8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03059</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96305.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VQX8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00159"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.600"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96305.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="48" mass="5829" checksum="7151C16B4E58D458" modified="2013-06-26" version="1">MLAKAILEEFRHRGIKVNTFYRKMIFIYTNSGIRDIESLFSYINKRLM</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="41" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR25</accession>
    <name>N6VR25_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">D-aminoacyl-tRNA deacylase</fullName>
            <ecNumber evidence="4">3.1.1.96</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4" type="primary">dtdA</name>
        <name evidence="5" type="ORF">J422_02729</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">D-aminoacyl-tRNA deacylase with broad substrate specificity. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>a D-aminoacyl-tRNA + H2O = a tRNA + a D-alpha-amino acid + H(+)</text>
            <dbReference type="Rhea" id="RHEA:13953"/>
            <dbReference type="Rhea" id="RHEA-COMP:10123"/>
            <dbReference type="Rhea" id="RHEA-COMP:10124"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:59871"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:79333"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="4">
            <text>glycyl-tRNA(Ala) + H2O = tRNA(Ala) + glycine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:53744"/>
            <dbReference type="Rhea" id="RHEA-COMP:9657"/>
            <dbReference type="Rhea" id="RHEA-COMP:13640"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:57305"/>
            <dbReference type="ChEBI" id="CHEBI:78442"/>
            <dbReference type="ChEBI" id="CHEBI:78522"/>
            <dbReference type="EC" id="3.1.1.96"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="4">
            <name>Zn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29105"/>
        </cofactor>
        <text evidence="4">Binds 2 Zn(2+) ions per subunit.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Monomer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the DtdA deacylase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.1.96" evidence="4"/>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96360.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590600.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR25"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00098"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.535"/>
    <dbReference type="OrthoDB" id="9863at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051499">
        <property type="term" value="F:D-aminoacyl-tRNA deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106026">
        <property type="term" value="F:Gly-tRNA(Ala) deacylase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019478">
        <property type="term" value="P:D-amino acid catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.10700">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.50">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00562">
        <property type="entry name" value="Deacylase_DtdA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018033">
        <property type="entry name" value="Deacylase_DtdA_archaea"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007508">
        <property type="entry name" value="DtdA"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34667:SF1">
        <property type="entry name" value="D-AMINOACYL-TRNA DEACYLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04414">
        <property type="entry name" value="tRNA_deacylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF016210">
        <property type="entry name" value="UCP016210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF142535">
        <property type="entry name" value="AF0625-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 4" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="1 4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 4" id="KW-0862">Zinc</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022833"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00562"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96360.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="241" mass="27750" checksum="EF1DA09373622CC7" modified="2013-06-26" version="1">MKFLFISSNLDPASKNIASFLNEYFEVFKVEKELLNVKKEDLPKASYYIFLSKHRSSSNQPSLTVHVPGLLDRDVCSASAVLNSLLLNNIYNFYRKSNLNGFNVCFEVVHHTPTDIDKPTCFVEIGSTEKEWKDERLGKIMADAIVKTVEQIESGDYDEKIKAVGYGGNHYAPKFTKLCLEGKYYFSYLIPKYANVSENVLKKIVKSDVERLLIDWKGSKSEGRKKMIEFFKSYNIDYERV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR46</accession>
    <name>N6VR46_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Sjogrens syndrome scleroderma autoantigen 1</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06972</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000066">
        <property type="protein sequence ID" value="ENN95596.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004594663.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000066.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR46"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01231"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1350"/>
    <dbReference type="OrthoDB" id="26305at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009563">
        <property type="entry name" value="SSSCA1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06677">
        <property type="entry name" value="Auto_anti-p27"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95596.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="96" mass="11083" checksum="8E6AD4A92ACEB8A5" modified="2013-06-26" version="1">MDPIKITSEELLKGAKMLSKHCEKCGFPLFEKDGKIYCAICNKSNLEDSHKLDKNDIIDKKIDYLLEKLKNENEISRIKEIGEAIAILIKIKKELY</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VR82</accession>
    <name>N6VR82_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Metallo-beta-lactamase domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_06673</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000057">
        <property type="protein sequence ID" value="ENN95651.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593904.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VR82"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00716"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1299"/>
    <dbReference type="OrthoDB" id="197151at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.60.15.10">
        <property type="entry name" value="Ribonuclease Z/Hydroxyacylglutathione hydrolase-like"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001279">
        <property type="entry name" value="Metallo-B-lactamas"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050855">
        <property type="entry name" value="NDM-1-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036866">
        <property type="entry name" value="RibonucZ/Hydroxyglut_hydro"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951">
        <property type="entry name" value="METALLO-BETA-LACTAMASE DOMAIN-CONTAINING"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42951:SF18">
        <property type="entry name" value="METALLO-HYDROLASE MJ0296-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00753">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00849">
        <property type="entry name" value="Lactamase_B"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56281">
        <property type="entry name" value="Metallo-hydrolase/oxidoreductase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Metallo-beta-lactamase" evidence="1">
        <location>
            <begin position="21"/>
            <end position="169"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="SMART" id="SM00849"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN95651.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="179" mass="20380" checksum="CF76A0F005D8D847" modified="2013-06-26" version="1">MIKLLYKGELIREGGIIKKASSSSTFISTEKHNILVDTSTKDKLKIILKNLPVKPDEIDVIINTHSHYDHVENNDFFKEATVYGSPKEGSYFEDYKKLKDKEIEIIETPGHTYGSISVIYKDYIIVGDASPLKDNILKLIPPRVNVDENLALKSLIKIRKLKKNVITGHDGCVKYSNFW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRG9</accession>
    <name>N6VRG9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Trm112 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_06140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000049">
        <property type="protein sequence ID" value="ENN95756.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593255.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000049.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRG9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00547"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1196"/>
    <dbReference type="OrthoDB" id="6467at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.25.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005651">
        <property type="entry name" value="Trm112-like"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03966">
        <property type="entry name" value="Trm112p"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158997">
        <property type="entry name" value="Trm112p-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95756.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="48" mass="5678" checksum="C2557146D5F913DA" modified="2013-06-26" version="1">MSWIEKYLEILQCPYCGGDLYLDSNKLVCKKCKRVFDIIEGIPILLRR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRP0</accession>
    <name>N6VRP0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11 12">Probable transaldolase</fullName>
            <ecNumber evidence="5 12">2.2.1.2</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="12" type="primary">tal</name>
        <name evidence="13" type="ORF">J422_01985</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="1 12">Transaldolase is important for the balance of metabolites in the pentose-phosphate pathway.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10 12">
            <text>D-sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-erythrose 4-phosphate + beta-D-fructose 6-phosphate</text>
            <dbReference type="Rhea" id="RHEA:17053"/>
            <dbReference type="ChEBI" id="CHEBI:16897"/>
            <dbReference type="ChEBI" id="CHEBI:57483"/>
            <dbReference type="ChEBI" id="CHEBI:57634"/>
            <dbReference type="ChEBI" id="CHEBI:59776"/>
            <dbReference type="EC" id="2.2.1.2"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="3 12">Carbohydrate degradation; pentose phosphate pathway; D-glyceraldehyde 3-phosphate and beta-D-fructose 6-phosphate from D-ribose 5-phosphate and D-xylulose 5-phosphate (non-oxidative stage): step 2/3.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 12">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="4 12">Belongs to the transaldolase family. Type 3B subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.2.1.2" evidence="5 12"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96540.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590162.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRP0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00242"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.389"/>
    <dbReference type="OrthoDB" id="6661at2157"/>
    <dbReference type="UniPathway" id="UPA00115">
        <property type="reaction ID" value="UER00414"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016832">
        <property type="term" value="F:aldehyde-lyase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004801">
        <property type="term" value="F:transaldolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005975">
        <property type="term" value="P:carbohydrate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006098">
        <property type="term" value="P:pentose-phosphate shunt"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd00956">
        <property type="entry name" value="Transaldolase_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000018">
        <property type="entry name" value="Probable transaldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00494">
        <property type="entry name" value="Transaldolase_3b"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001585">
        <property type="entry name" value="TAL/FSA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022999">
        <property type="entry name" value="Transaldolase_3B"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004731">
        <property type="entry name" value="Transaldolase_3B/F6P_aldolase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018225">
        <property type="entry name" value="Transaldolase_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR033919">
        <property type="entry name" value="TSA/FSA_arc/bac"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00875">
        <property type="entry name" value="fsa_talC_mipB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683:SF40">
        <property type="entry name" value="FRUCTOSE-6-PHOSPHATE ALDOLASE 1-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10683">
        <property type="entry name" value="TRANSALDOLASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00923">
        <property type="entry name" value="TAL_FSA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS01054">
        <property type="entry name" value="TRANSALDOLASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00958">
        <property type="entry name" value="TRANSALDOLASE_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6 12" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 12" id="KW-0570">Pentose shunt</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="9 12" id="KW-0704">Schiff base</keyword>
    <keyword evidence="7 12 13" id="KW-0808">Transferase</keyword>
    <feature type="active site" description="Schiff-base intermediate with substrate" evidence="12">
        <location>
            <position position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00003518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00005740"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00013151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023126"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023270"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00048810"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00067532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96540.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="219" mass="24106" checksum="D8578DAD2C342BA5" modified="2013-06-26" version="1">MKFFLDTANINEIKKYAELGLIDGVTTNPTLVAKEKREFKELIKEICSIVDGPVSAEVISTDAEGMIKEAEELSKIADNIVVKIPMTRDGLKAVKVLSEEGIDTNVTLVFSPLQALLAAKAGATYVSPFIGRLDDIGHVGMGLVEDVVLIFNNYGIETEVIVSSIRHPWHVLEAAKIGADIATIPPSVMEKLFNHPLTDIGLERFLKDWEEYKRVIKGD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="17" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRV0</accession>
    <name>N6VRV0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_01445</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000010">
        <property type="protein sequence ID" value="ENN96585.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589950.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRV0"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.283"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96585.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="65" mass="7672" checksum="D26D022B7FAA1A88" modified="2013-06-26" version="1">MEGSDLNWINAQSERLKNLKEKYHLVILTLDYVNDIEMTKKCIEHAKKYNFIPMTTKDITLNSIN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="37" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRV6</accession>
    <name>N6VRV6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Polyferredoxin MvhB</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_01495</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000010">
        <property type="protein sequence ID" value="ENN96595.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589960.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000010.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRV6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01033"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.294"/>
    <dbReference type="OrthoDB" id="23478at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016491">
        <property type="term" value="F:oxidoreductase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-ARBA"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.20">
        <property type="match status" value="7"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017896">
        <property type="entry name" value="4Fe4S_Fe-S-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017900">
        <property type="entry name" value="4Fe4S_Fe_S_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050572">
        <property type="entry name" value="Fe-S_Ferredoxin"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687">
        <property type="entry name" value="ADENYLYLSULFATE REDUCTASE, BETA SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43687:SF6">
        <property type="entry name" value="L-ASPARTATE SEMIALDEHYDE SULFURTRANSFERASE IRON-SULFUR SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00037">
        <property type="entry name" value="Fer4"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF12838">
        <property type="entry name" value="Fer4_7"/>
        <property type="match status" value="3"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13187">
        <property type="entry name" value="Fer4_9"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54862">
        <property type="entry name" value="4Fe-4S ferredoxins"/>
        <property type="match status" value="4"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00198">
        <property type="entry name" value="4FE4S_FER_1"/>
        <property type="match status" value="6"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51379">
        <property type="entry name" value="4FE4S_FER_2"/>
        <property type="match status" value="11"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="5" id="KW-0249">Electron transport</keyword>
    <keyword evidence="6" id="KW-0408">Iron</keyword>
    <keyword evidence="7" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="3" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4" id="KW-0677">Repeat</keyword>
    <keyword evidence="1" id="KW-0813">Transport</keyword>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="35"/>
            <end position="66"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="67"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="97"/>
            <end position="126"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="167"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="212"/>
            <end position="243"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="250"/>
            <end position="279"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="280"/>
            <end position="309"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="335"/>
            <end position="365"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="366"/>
            <end position="395"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="405"/>
            <end position="429"/>
        </location>
    </feature>
    <feature type="domain" description="4Fe-4S ferredoxin-type" evidence="8">
        <location>
            <begin position="430"/>
            <end position="459"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022737"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022982"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51379"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96595.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="464" mass="52312" checksum="E8D52F0BEF84AB43" modified="2013-06-26" version="1">MIITLLDKCRPKDECKSCPFYTKTSKCIEACPTDAIFLLNNKSFSCLTCGQCAEACPNKAIRKNEFGGYYVDRKRCNGCGLCEKACPIGIIKLVEKGGRKFPMGICSMCGACVEVCPYNARVSSYELLNTKRDKLADIYLKALSKILKVDLKRENIEEMRKIKEKRVNIKIDKDKCVGCLRCSYLCPKDTIKPINVNACTLCNLCHEHCPVDAIEYGEVKEGCILCFNCVNICPNSVLKVENFKVVKLKEDKKIKPKNYCINCGLCALECKSGALKFEDNRLYYSPDLCWKCLKCVEVCPQNVRELKGDIIIGGCSLCEICINNCPENAIEIEEKIFENIKDDRCIVCGTCSNVCPMNAIIIDKTNKTIMFKDNCIACENCSIHCPRDIIPNTTGFKKVVDKERSFIRTDMSLCIKCGLCNKACPNECIDYGVIDYDKCEFCGACYNTCPVKAIYLYRSWKIKE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VRZ1</accession>
    <name>N6VRZ1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">DUF2121 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01086</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96645.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589885.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VRZ1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00825"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.212"/>
    <dbReference type="OrthoDB" id="106876at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016754">
        <property type="entry name" value="MJ0548-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057377">
        <property type="entry name" value="MJ0548_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR057262">
        <property type="entry name" value="MJ0548_N"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25274">
        <property type="entry name" value="MJ0548_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09894">
        <property type="entry name" value="MJ0548_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF019262">
        <property type="entry name" value="UCP019262"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Connectase MJ0548-like N-terminal" evidence="1">
        <location>
            <begin position="1"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Connectase MJ0548-like C-terminal" evidence="2">
        <location>
            <begin position="197"/>
            <end position="290"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09894"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF25274"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96645.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="5">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="292" mass="33727" checksum="1AA3D24DBAF495A7" modified="2013-06-26" version="1">MSLVICYYGNNGAVMAGDRRQMFFKGPEEKRKILEEKLYSGEIQNEEDLYKLADDLGVKVIIEDNREKVRKIGNTLVGEVRSIGLEAKRRRVYATKGKCMILEILGDVITDRMLKNGAGLIVFGNRYLKNKAEKILKNVAKDFPKMDIDEVGKVIKDVFERFKEHPTISREYDIYVTKNIDINFEKTVEEDINKLFKYREDIRKKMIDFGKVMSIVNKIVKNGEVGVIKEGKLHLYDQYIAIDKISPNFKTFRIIDVKGDVEDGDIVVIENGDMKIKNKGIKVMTDYIICYK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS04</accession>
    <name>N6VS04_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">3-dehydroquinate synthase</fullName>
            <shortName evidence="5">DHQ synthase</shortName>
            <ecNumber evidence="5">1.4.1.24</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="5">3-dehydroquinate synthase II</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="5" type="primary">aroB'</name>
        <name evidence="8" type="ORF">J422_01171</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Catalyzes the oxidative deamination and cyclization of 2-amino-3,7-dideoxy-D-threo-hept-6-ulosonic acid (ADH) to yield 3-dehydroquinate (DHQ), which is fed into the canonical shikimic pathway of aromatic amino acid biosynthesis.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>2-amino-2,3,7-trideoxy-D-lyxo-hept-6-ulosonate + NAD(+) + H2O = 3-dehydroquinate + NH4(+) + NADH + H(+)</text>
            <dbReference type="Rhea" id="RHEA:25956"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:28938"/>
            <dbReference type="ChEBI" id="CHEBI:32364"/>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
            <dbReference type="ChEBI" id="CHEBI:57945"/>
            <dbReference type="ChEBI" id="CHEBI:58859"/>
            <dbReference type="EC" id="1.4.1.24"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the archaeal-type DHQ synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.4.1.24" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96660.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589900.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS04"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00810"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.229"/>
    <dbReference type="OrthoDB" id="10265at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003856">
        <property type="term" value="F:3-dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102042">
        <property type="term" value="F:dehydroquinate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051287">
        <property type="term" value="F:NAD binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008652">
        <property type="term" value="P:amino acid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009073">
        <property type="term" value="P:aromatic amino acid family biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01244">
        <property type="entry name" value="Arch_DHQ_synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002812">
        <property type="entry name" value="DHQS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030960">
        <property type="entry name" value="DHQS/DOIS_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056179">
        <property type="entry name" value="DHQS_C"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002624">
        <property type="entry name" value="PRK02290.1-2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002626">
        <property type="entry name" value="PRK02290.1-4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002627">
        <property type="entry name" value="PRK02290.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33563">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR33563:SF1">
        <property type="entry name" value="3-DEHYDROQUINATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01959">
        <property type="entry name" value="DHQS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26558">
        <property type="entry name" value="DHQS_2nd"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF006655">
        <property type="entry name" value="DHQ_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 5" id="KW-0028">Amino-acid biosynthesis</keyword>
    <keyword evidence="4 5" id="KW-0057">Aromatic amino acid biosynthesis</keyword>
    <keyword evidence="8" id="KW-0456">Lyase</keyword>
    <keyword evidence="3 5" id="KW-0520">NAD</keyword>
    <keyword evidence="2 5" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="3-dehydroquinate synthase N-terminal" evidence="6">
        <location>
            <begin position="1"/>
            <end position="173"/>
        </location>
    </feature>
    <feature type="domain" description="3-dehydroquinate synthase C-terminal" evidence="7">
        <location>
            <begin position="187"/>
            <end position="361"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022605"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01244"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="Pfam" id="PF01959"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="Pfam" id="PF26558"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96660.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="361" mass="40323" checksum="367CD81C4FA46AAF" modified="2013-06-26" version="1">MKFGWIKIDGDDWEEKKEKVKMALESSIPVVVADPEDIEKIKELGNIKVASHSLDADIVIINKNDDINFLKKAKELGKETAVFIPIEGKEDEELASEIARFGFVDNIILKGEDWKIIPLENLIADLFNRDVKIIACVDDVKEAKTAYEILEKGTDGVVLSPKSLEDIKELSKLIDEMNKEKIKLDTAVIKRVEPIGSGDRVCIDTCSLMSIGEGMLVGSYSRGFFLVHSETVENPYVETRPFRVNAGAVHSYILCPGNKTKYLSELKAGDKVLIVDKDGNAREAIVGRVKIERRPLMLVEAEYKGDIVRVILQNAETIRLVGKDGNPISIVDLKPGDEVLIKYDESARHFGMAIKEKIIEK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VS23</accession>
    <name>N6VS23_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">Methyltransferase small</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_04920</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95956.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592083.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VS23"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00507"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.964"/>
    <dbReference type="OrthoDB" id="4668at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008757">
        <property type="term" value="F:S-adenosylmethionine-dependent methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046977">
        <property type="entry name" value="RsmC/RlmG"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007848">
        <property type="entry name" value="Small_mtfrase_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47816">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47816:SF4">
        <property type="entry name" value="RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05175">
        <property type="entry name" value="MTS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1 4" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Methyltransferase small" evidence="3">
        <location>
            <begin position="26"/>
            <end position="192"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022603"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF05175"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN95956.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="197" mass="22273" checksum="61A56DC5F9861045" modified="2013-06-26" version="1">MHYFSEKPTTKSNIKIIEDILRGKLLKFKTDSGVFSSDKIDKGTKILVESVEVNKNDEILDLGCGYGVIGIALADEVKSVLMSDINRRALKLAKENIKLNNLSDKYIDVVYSDLFNNINKRFDKIITNPPIRAGKEVLNRIIGEGKDYLKDGGEIWLVIRTKQGAKSLANYMEEIFGNVETVEIKGGYRVLKSKKQW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSD0</accession>
    <name>N6VSD0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Peptidyl-prolyl cis-trans isomerase</fullName>
            <ecNumber evidence="6">5.2.1.8</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="8" type="ORF">J422_00421</name>
    </gene>
    <organism evidence="8 9">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="8 9" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="8 9">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1 5 6">
            <text>[protein]-peptidylproline (omega=180) = [protein]-peptidylproline (omega=0)</text>
            <dbReference type="Rhea" id="RHEA:16237"/>
            <dbReference type="Rhea" id="RHEA-COMP:10747"/>
            <dbReference type="Rhea" id="RHEA-COMP:10748"/>
            <dbReference type="ChEBI" id="CHEBI:83833"/>
            <dbReference type="ChEBI" id="CHEBI:83834"/>
            <dbReference type="EC" id="5.2.1.8"/>
        </reaction>
    </comment>
    <comment type="similarity">
        <text evidence="2 6">Belongs to the FKBP-type PPIase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="8">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="5.2.1.8" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96780.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589766.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSD0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00696"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.81"/>
    <dbReference type="OrthoDB" id="8615at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003755">
        <property type="term" value="F:peptidyl-prolyl cis-trans isomerase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.40.10.330">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.10.50.40">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.70.2210">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040825">
        <property type="entry name" value="FKBP26_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054016">
        <property type="entry name" value="FKBP26_IF"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR046357">
        <property type="entry name" value="PPIase_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001179">
        <property type="entry name" value="PPIase_FKBP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR048261">
        <property type="entry name" value="SlpA/SlyD-like_ins_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861">
        <property type="entry name" value="FKBP-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE SLYD"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR47861:SF2">
        <property type="entry name" value="LONG-TYPE PEPTIDYL-PROLYL CIS-TRANS ISOMERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF18046">
        <property type="entry name" value="FKBP26_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22199">
        <property type="entry name" value="FKBP26_IF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00254">
        <property type="entry name" value="FKBP_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54534">
        <property type="entry name" value="FKBP-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50059">
        <property type="entry name" value="FKBP_PPIASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5 6" id="KW-0413">Isomerase</keyword>
    <keyword evidence="9" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 5 6" id="KW-0697">Rotamase</keyword>
    <feature type="domain" description="PPIase FKBP-type" evidence="7">
        <location>
            <begin position="5"/>
            <end position="92"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000971"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00006577"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00023110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023235"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00277"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="RuleBase" id="RU003915"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="7">
        <source>
            <dbReference type="PROSITE" id="PS50059"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="EMBL" id="ENN96780.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="222" mass="25101" checksum="5B59C7A90E5218E2" modified="2013-06-26" version="1">MVEKGALIKISYDGYVDGKLFDTTNEELAKKEGIYNPNIVYGPVPIFAGEGMVVKGLDEILLEMDVGEEKEVVLPPEKAFGKRDPAKIKLVPMREFIKRGIKPIVGLRVNIDGEIGRIVKAEGGRVLVDFNHELAGKEVKYRIKIEEKVEGDENIIREIFNMFAPKIKPNVTIEGEKAIIELPEEAFLINLQQIKLAVANEVFKRLKNIKEIKFIETVKRKD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="34" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSH8</accession>
    <name>N6VSH8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="9">Methyl-accepting chemotaxis sensory transducer</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="9" type="ORF">J422_04093</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1">Membrane</location>
            <topology evidence="1">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the methyl-accepting chemotaxis (MCP) protein family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000027">
        <property type="protein sequence ID" value="ENN96111.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSH8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01356"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0007165">
        <property type="term" value="P:signal transduction"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd06225">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Gene3D" id="6.10.340.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.287.950">
        <property type="entry name" value="Methyl-accepting chemotaxis protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003660">
        <property type="entry name" value="HAMP_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029095">
        <property type="entry name" value="NarX-like_N"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089:SF112">
        <property type="entry name" value="LYSOZYME-LIKE PROTEIN-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR32089">
        <property type="entry name" value="METHYL-ACCEPTING CHEMOTAXIS PROTEIN MCPB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00672">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13675">
        <property type="entry name" value="PilJ"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00304">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF158472">
        <property type="entry name" value="HAMP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF58104">
        <property type="entry name" value="Methyl-accepting chemotaxis protein (MCP) signaling domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50885">
        <property type="entry name" value="HAMP"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0175">Coiled coil</keyword>
    <keyword evidence="4 7" id="KW-0472">Membrane</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 7" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="3 7" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="15"/>
            <end position="37"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="7">
        <location>
            <begin position="173"/>
            <end position="196"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="198"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="domain" description="HAMP" evidence="8">
        <location>
            <begin position="258"/>
            <end position="308"/>
        </location>
    </feature>
    <feature type="coiled-coil region" evidence="6">
        <location>
            <begin position="293"/>
            <end position="369"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="9">
        <location>
            <position position="371"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004141"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00029447"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="SAM" id="Coils"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS50885"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN96111.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="371" mass="42156" checksum="0C32F7F89B320C59" modified="2013-06-26" version="1" fragment="single">MGGILLKNIPIKYKLLGIVLALVIINLITGGLVLCVIDCMKKDAEIMNIASMERSLIKDMSKYTTMISYGEDVKNVLKEKSDMFEKNLNTLLYGDKERGIPEASGEFKDQLLKVKKLWKEYKENINVVLESSPGDPNFLEAVNYIRNNSKVLFNEQNKAVMIYQKNSEEKIELVKTIVIIMMVIAIIIGALSYYVVKVAIIAPIMDLKRMLMEVVNGNYDVKPKIKFGNDELGDLEKCFLHMINKIKELIETIDSDRKAIRKTFKELREAMDRLAKGDLTVRLEVKDKRSKAQEAFNRAVESMQNLIKSLRQEIINLNKEINALREETQRAKETAEQVADAANQVAVAATDQSNKLQDLTQEVEDTAKMAE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="36" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSI6</accession>
    <name>N6VSI6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cobalt transport protein CbiN</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Energy-coupling factor transporter probable substrate-capture protein CbiN</fullName>
            <shortName evidence="10">ECF transporter S component CbiN</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cbiN</name>
        <name evidence="11" type="ORF">J422_00270</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Part of the energy-coupling factor (ECF) transporter complex CbiMNOQ involved in cobalt import.</text>
    </comment>
    <comment type="pathway">
        <text evidence="10">Cofactor biosynthesis; adenosylcobalamin biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="10">Forms an energy-coupling factor (ECF) transporter complex composed of an ATP-binding protein (A component, CbiO), a transmembrane protein (T component, CbiQ) and 2 possible substrate-capture proteins (S components, CbiM and CbiN) of unknown stoichimetry.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="10">Cell membrane</location>
            <topology evidence="10">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the CbiN family.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96835.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589741.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSI6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01109"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.54"/>
    <dbReference type="OrthoDB" id="187156at2157"/>
    <dbReference type="UniPathway" id="UPA00148"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015087">
        <property type="term" value="F:cobalt ion transmembrane transporter activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009236">
        <property type="term" value="P:cobalamin biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00330">
        <property type="entry name" value="CbiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003705">
        <property type="entry name" value="CbiN"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01165">
        <property type="entry name" value="cbiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF002780">
        <property type="entry name" value="PRK02898.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38662">
        <property type="entry name" value="COBALT TRANSPORT PROTEIN CBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38662:SF1">
        <property type="entry name" value="COBALT TRANSPORT PROTEIN CBIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02553">
        <property type="entry name" value="CbiN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 10" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="4 10" id="KW-0169">Cobalamin biosynthesis</keyword>
    <keyword evidence="9 10" id="KW-0170">Cobalt</keyword>
    <keyword evidence="1 10" id="KW-0171">Cobalt transport</keyword>
    <keyword evidence="7 10" id="KW-0406">Ion transport</keyword>
    <keyword evidence="8 10" id="KW-0472">Membrane</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="5 10" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="6 10" id="KW-1133">Transmembrane helix</keyword>
    <keyword evidence="2 10" id="KW-0813">Transport</keyword>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="7"/>
            <end position="27"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="10">
        <location>
            <begin position="63"/>
            <end position="83"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022426"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022573"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023285"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00330"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN96835.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="91" mass="10089" checksum="1A15BA26596C4341" modified="2013-06-26" version="1">METKHIVLLGIIILLCVIPLAFVKGSFEGSDDQAEDVIKDVNPQYHPWFQPIWEPPSGEIESLLFAVQAAIGAIIIGYFIGYYKGKAESTH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSI8</accession>
    <name>N6VSI8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">PHP domain-containing protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_04043</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000026">
        <property type="protein sequence ID" value="ENN96126.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591523.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000026.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSI8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00722"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.792"/>
    <dbReference type="OrthoDB" id="114814at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd19067">
        <property type="entry name" value="PfuEndoQ-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.140">
        <property type="entry name" value="Metal-dependent hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005287">
        <property type="entry name" value="CHP00375"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016195">
        <property type="entry name" value="Pol/histidinol_Pase-like"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00375">
        <property type="entry name" value="TIGR00375 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40084">
        <property type="entry name" value="PHOSPHOHYDROLASE, PHP FAMILY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40084:SF1">
        <property type="entry name" value="PHOSPHOTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF89550">
        <property type="entry name" value="PHP domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96126.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="364" mass="42512" checksum="73BD010E31BB72D5" modified="2013-06-26" version="1" fragment="single">KYSKLKGLDIVGTGDCLQKDYLEEIKEFKDRELILTTEVEDKDRVHHLIILPSIEKAEELRENFKKYSKNLNEGRPKVFLSGAEILEMVRDVDGLIGPAHAFTPWTSLYKSFDSIYQCYEKKPDFVELGLSADTDMADSIKELREFPFLTNSDAHSYYPHRLGREFNQFEIDSVGGLEENFEEIEEAIKKNKIVANYGLDPRLGKYHLTACSKCHTRFKLEDAKKYNWRCPKCKGIIKKGVLSRVEELSDGRVEHPPFRPPYYKIIPLAEIISLTYNKNVFSKFVQNLWERFIRSFKNEIEVLINRDIDELSKIDEKVAKTIELFRENKIYIYPGGGGEYGKISFVPQKVNYYKEEITLDRWLS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="43" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSJ2</accession>
    <name>N6VSJ2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="14">Probable phosphoenolpyruvate synthase</fullName>
            <ecNumber evidence="5">2.7.9.2</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">Pyruvate, water dikinase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="18" type="ORF">J422_00295</name>
    </gene>
    <organism evidence="18 19">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="18 19" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="18 19">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="2">Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="13">
            <text>pyruvate + ATP + H2O = phosphoenolpyruvate + AMP + phosphate + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:11364"/>
            <dbReference type="ChEBI" id="CHEBI:15361"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58702"/>
            <dbReference type="ChEBI" id="CHEBI:456215"/>
            <dbReference type="EC" id="2.7.9.2"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="3">Carbohydrate biosynthesis; gluconeogenesis.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the PEP-utilizing enzyme family.</text>
    </comment>
    <comment type="caution">
        <text evidence="18">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.9.2" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96840.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSJ2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01104"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.59"/>
    <dbReference type="UniPathway" id="UPA00138"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008986">
        <property type="term" value="F:pyruvate, water dikinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006094">
        <property type="term" value="P:gluconeogenesis"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniPathway"/>
    </dbReference>
    <dbReference type="FunFam" id="3.30.1490.20:FF:000010">
        <property type="entry name" value="Phosphoenolpyruvate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.60">
        <property type="entry name" value="Phosphoenolpyruvate-binding domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.50.30.10">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008279">
        <property type="entry name" value="PEP-util_enz_mobile_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006319">
        <property type="entry name" value="PEP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR018274">
        <property type="entry name" value="PEP_util_AS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000121">
        <property type="entry name" value="PEP_util_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023151">
        <property type="entry name" value="PEP_util_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036637">
        <property type="entry name" value="Phosphohistidine_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002192">
        <property type="entry name" value="PPDK_AMP/ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015813">
        <property type="entry name" value="Pyrv/PenolPyrv_kinase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR040442">
        <property type="entry name" value="Pyrv_kinase-like_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01418">
        <property type="entry name" value="PEP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF005057">
        <property type="entry name" value="PRK06464.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43030:SF1">
        <property type="entry name" value="PHOSPHOENOLPYRUVATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00391">
        <property type="entry name" value="PEP-utilizers"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02896">
        <property type="entry name" value="PEP-utilizers_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01326">
        <property type="entry name" value="PPDK_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR01736">
        <property type="entry name" value="PHPHTRNFRASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51621">
        <property type="entry name" value="Phosphoenolpyruvate/pyruvate domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52009">
        <property type="entry name" value="Phosphohistidine domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00742">
        <property type="entry name" value="PEP_ENZYMES_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00370">
        <property type="entry name" value="PEP_ENZYMES_PHOS_SITE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="9" id="KW-0418">Kinase</keyword>
    <keyword evidence="11" id="KW-0460">Magnesium</keyword>
    <keyword evidence="7" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="8" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="18" id="KW-0670">Pyruvate</keyword>
    <keyword evidence="19" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="6 18" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate phosphate dikinase AMP/ATP-binding" evidence="16">
        <location>
            <begin position="16"/>
            <end position="329"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme mobile" evidence="15">
        <location>
            <begin position="363"/>
            <end position="433"/>
        </location>
    </feature>
    <feature type="domain" description="PEP-utilising enzyme C-terminal" evidence="17">
        <location>
            <begin position="458"/>
            <end position="707"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00002988"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004742"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00007837"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00011996"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00033470"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00047700"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00071420"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="15">
        <source>
            <dbReference type="Pfam" id="PF00391"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="16">
        <source>
            <dbReference type="Pfam" id="PF01326"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="17">
        <source>
            <dbReference type="Pfam" id="PF02896"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="18">
        <source>
            <dbReference type="EMBL" id="ENN96840.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="19">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="718" mass="79822" checksum="96723A1955EA2FE7" modified="2013-06-26" version="1">MKFIAWLDELSNKDVSIAGGKGASLGEMWNAGLPVPPAFVVTADAYRYFIKETGLMEKIKQVLKDLDINNTKALEEASKKIRSMIESEKMPDDLRLAIIEAYNKLCEMCGEEVFVAVRSSATAEDLPDASFAGQQETFLNVKGAENVVKYVQKCFSSLFTPRAIFYREQKGFDHFKVALAAVVQKMVNADKAGVMFTVNPITEKENELVIEAAWGLGEGVVSGAVSPDTYIVDKNTLKILDKHIAKKEIMYVRDEKGETKVVEVPEDMKEKQVLTDDEIVELTEMGIKIEKHYGMPMDVEWAYEGKKCYMLQARPITTLGKRKKKVSEEVEGKILLKGIGASPGIASGRVKIVMDIEEIDKVEEGDILVTKMTTPDMVPAMKKAAAIVTDEGGLTCHAAIVSRELGVPCVVGTKYATKILKDGMLVTVDGEKGVVYEGIIKKEEEEKKEVIVKESPTIITATEVKVNVSMPEAAEKAAKTGADGVGLLRAEHMILGLGKHPKKILEDEGREALINALMEGIRKVADAFYPRPVTYRTLDAPTDEFRNLEGGDREPIEHNPMLGWRGIRRSLDDPDILKCELEAIKRLREEGYKNIEIMIPLVTHPDEVRKVKEIMREVGLEPGKDIKFGIMVETPAAALIIEEFIKEGIDFISLGTNDLTQYTIAIDRNNELVAKYYKEDHPAVLKLVEYVIKTCKKHGVKTSICGKQDQGHILLKSW</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="46" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSP3</accession>
    <name>N6VSP3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="6">Phosphoribosylformylglycinamidine synthase subunit PurS</fullName>
            <shortName evidence="6">FGAM synthase</shortName>
            <ecNumber evidence="6">6.3.5.3</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="6">Formylglycinamide ribonucleotide amidotransferase subunit III</fullName>
            <shortName evidence="6">FGAR amidotransferase III</shortName>
            <shortName evidence="6">FGAR-AT III</shortName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="6">Phosphoribosylformylglycinamidine synthase subunit III</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="6" type="primary">purS</name>
        <name evidence="7" type="ORF">J422_03628</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6">Part of the phosphoribosylformylglycinamidine synthase complex involved in the purines biosynthetic pathway. Catalyzes the ATP-dependent conversion of formylglycinamide ribonucleotide (FGAR) and glutamine to yield formylglycinamidine ribonucleotide (FGAM) and glutamate. The FGAM synthase complex is composed of three subunits. PurQ produces an ammonia molecule by converting glutamine to glutamate. PurL transfers the ammonia molecule to FGAR to form FGAM in an ATP-dependent manner. PurS interacts with PurQ and PurL and is thought to assist in the transfer of the ammonia molecule from PurQ to PurL.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="9">
            <text>N(2)-formyl-N(1)-(5-phospho-beta-D-ribosyl)glycinamide + L-glutamine + ATP + H2O = 2-formamido-N(1)-(5-O-phospho-beta-D-ribosyl)acetamidine + L-glutamate + ADP + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:17129"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29985"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58359"/>
            <dbReference type="ChEBI" id="CHEBI:147286"/>
            <dbReference type="ChEBI" id="CHEBI:147287"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.5.3"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="6">Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2.</text>
    </comment>
    <comment type="subunit">
        <text evidence="6">Part of the FGAM synthase complex composed of 1 PurL, 1 PurQ and 2 PurS subunits.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="6">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="6">Belongs to the PurS family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.5.3" evidence="6"/>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96206.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591262.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000023.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSP3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01378"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.708"/>
    <dbReference type="OrthoDB" id="56303at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00128"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004642">
        <property type="term" value="F:phosphoribosylformylglycinamidine synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1280.10">
        <property type="entry name" value="Phosphoribosylformylglycinamidine synthase subunit PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01926">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003850">
        <property type="entry name" value="PurS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036604">
        <property type="entry name" value="PurS-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00302">
        <property type="entry name" value="phosphoribosylformylglycinamidine synthase subunit PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004630">
        <property type="entry name" value="PRK05974.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR34696:SF1">
        <property type="entry name" value="PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE SUBUNIT PURS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02700">
        <property type="entry name" value="PurS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF82697">
        <property type="entry name" value="PurS-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1 6" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="2 6 7" id="KW-0436">Ligase</keyword>
    <keyword evidence="3 6" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="4 6" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01926"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96206.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="84" mass="9700" checksum="5DE4CA99EA1CC2BF" modified="2013-06-26" version="1">MKYKATVVIRLKKGLLNPEGKAIKKALNFLNFDVEDVDSYKMLDIILNAENEEDAKKKVEEMCKKLLANPVIHNYYIKIESLSQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSQ4</accession>
    <name>N6VSQ4_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Methyltransferase type 11</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03723</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000023">
        <property type="protein sequence ID" value="ENN96221.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSQ4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01361"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.727"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008168">
        <property type="term" value="F:methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032259">
        <property type="term" value="P:methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42873">
        <property type="entry name" value="RIBOSOMAL RNA LARGE SUBUNIT METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42873:SF1">
        <property type="entry name" value="S-ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0489">Methyltransferase</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0808">Transferase</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96221.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="99" mass="11359" checksum="69321760282A26AB" modified="2013-06-26" version="1" fragment="single">DPPAFAQKEEDVKRALKGYSALNYLGMKLAEKLFVTCSCSHHIDRELFKKTVFSSAYRAGKELFIIDYRGQAPDHPVTVGNKHLEYLKCLFAYINKIFN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSR0</accession>
    <name>N6VSR0_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">Glycosyltransferase 2-like domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03503</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000022">
        <property type="protein sequence ID" value="ENN96231.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591153.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSR0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01333"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.683"/>
    <dbReference type="OrthoDB" id="11098at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006487">
        <property type="term" value="P:protein N-linked glycosylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001173">
        <property type="entry name" value="Glyco_trans_2-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859:SF91">
        <property type="entry name" value="DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10859">
        <property type="entry name" value="GLYCOSYL TRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00535">
        <property type="entry name" value="Glycos_transf_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Glycosyltransferase 2-like" evidence="1">
        <location>
            <begin position="4"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF00535"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96231.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="241" mass="28131" checksum="FD1415E30AA7EF3E" modified="2013-06-26" version="1">MELSIIMPCYKGEKFIENSIRTTEKIVSEFCKDFEIIVVVDGFVDNSYRIAKNLEKEFKNLKVVGYEKNRGKGFAIRYGLKFAEGEYIAFLDSDLDICPSSLKYYFETMKKENVDMVIGNRRDKNSVWEYPKLRKFLSYCFNIYVNIIFPELRLNDTQTGIKMFKREVIKDIFSNLPNFNSIDGYTFDIAILVLARKKGYKIKEMPCIVKKEDAQNISGVNLLKVIYKMGKDVIQLKRILG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="25" xmlns="https://uniprot.org/uniprot">
    <accession>N6VST9</accession>
    <name>N6VST9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">FeoA family protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_03368</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96266.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590979.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VST9"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01059"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.660"/>
    <dbReference type="OrthoDB" id="105333at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046914">
        <property type="term" value="F:transition metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.90">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007167">
        <property type="entry name" value="Fe-transptr_FeoA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR053184">
        <property type="entry name" value="FeoA-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038157">
        <property type="entry name" value="FeoA_core_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008988">
        <property type="entry name" value="Transcriptional_repressor_C"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43151">
        <property type="entry name" value="FEOA FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43151:SF1">
        <property type="entry name" value="SSR2333 PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04023">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00899">
        <property type="entry name" value="FeoA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF50037">
        <property type="entry name" value="C-terminal domain of transcriptional repressors"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0408">Iron</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Ferrous iron transporter FeoA-like" evidence="2">
        <location>
            <begin position="2"/>
            <end position="72"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="SMART" id="SM00899"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96266.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="74" mass="7858" checksum="284A776413593602" modified="2013-06-26" version="1">MMSLADVEEGKVVVIKDILAGHGLKNRLQSMGLVIGDKVKVLKNSHGPVIVENRGVKIALGRGVAMKIIVGDIK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="27" xmlns="https://uniprot.org/uniprot">
    <accession>N6VSU3</accession>
    <name>N6VSU3_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">EamA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_03393</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96271.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590989.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VSU3"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01064"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.666"/>
    <dbReference type="OrthoDB" id="65843at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016020">
        <property type="term" value="C:membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.3730.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000620">
        <property type="entry name" value="EamA_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR037185">
        <property type="entry name" value="EmrE-like"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911">
        <property type="entry name" value="ACYL-MALONYL CONDENSING ENZYME-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR22911:SF137">
        <property type="entry name" value="SOLUTE CARRIER FAMILY 35 MEMBER G2-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00892">
        <property type="entry name" value="EamA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF103481">
        <property type="entry name" value="Multidrug resistance efflux transporter EmrE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="31"/>
            <end position="50"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="62"/>
            <end position="84"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="90"/>
            <end position="109"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="133"/>
        </location>
    </feature>
    <feature type="domain" description="EamA" evidence="2">
        <location>
            <begin position="4"/>
            <end position="133"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF00892"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96271.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="134" mass="14790" checksum="04B0D3430371A5F0" modified="2013-06-26" version="1">MDEIILGLLVAIFYGISTFFAKIVCNENPIFQWVVVNIVGIILCLILLLYFKDVFVFDKKILIFAILSAVFVVFGSLLLYYALYKGKASIVVPISSVGPAITVVLSIIFLKESLTINQIIGIVLIMIGVIMLSI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="22" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT01</accession>
    <name>N6VT01_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_03164</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96326.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590794.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT01"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00179"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.621"/>
    <dbReference type="OrthoDB" id="59960at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056730">
        <property type="entry name" value="DUF2096_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056731">
        <property type="entry name" value="DUF2096_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017098">
        <property type="entry name" value="UCP037052"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23100">
        <property type="entry name" value="DUF2096_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF09869">
        <property type="entry name" value="KH_DUF2096_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF037052">
        <property type="entry name" value="UCP037052"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="DUF2096" evidence="2">
        <location>
            <begin position="7"/>
            <end position="96"/>
        </location>
    </feature>
    <feature type="domain" description="DUF2096" evidence="1">
        <location>
            <begin position="116"/>
            <end position="173"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="Pfam" id="PF09869"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="Pfam" id="PF23100"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96326.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="175" mass="20460" checksum="A434CCB0CAC575DD" modified="2013-06-26" version="1">MKDARNLEKIWVVLSEMSAELVNKNIPVPEDVFDKLRLANSMISYYLLDPHVDAKLLIEIEKVLNNIQSKLFTLCDEELMNIYLNKLNKAIRGELEVSFPISKSNYNKEVLRKGNVERVRIKLQKDIAIERLGELGEWYGVIFEYSEEKDKILIEGEINRIKTLLKDFSVIWKSD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT50</accession>
    <name>N6VT50_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="2">Replication factor C small subunit</fullName>
        </recommendedName>
        <alternativeName>
            <fullName evidence="8">Clamp loader small subunit</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_02814</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the activator 1 small subunits family. RfcS subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000017">
        <property type="protein sequence ID" value="ENN96371.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590622.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000017.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT50"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00112"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.552"/>
    <dbReference type="OrthoDB" id="7928at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005663">
        <property type="term" value="C:DNA replication factor C complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003677">
        <property type="term" value="F:DNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003689">
        <property type="term" value="F:DNA clamp loader activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0009378">
        <property type="term" value="F:four-way junction helicase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006310">
        <property type="term" value="P:DNA recombination"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006281">
        <property type="term" value="P:DNA repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006261">
        <property type="term" value="P:DNA-templated DNA replication"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016539">
        <property type="term" value="P:intein-mediated protein splicing"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd00009">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="CDD" id="cd00081">
        <property type="entry name" value="Hint"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="CDD" id="cd18140">
        <property type="entry name" value="HLD_clamp_RFC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.20.272.10:FF:000029">
        <property type="entry name" value="Replication factor C small subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.8.60:FF:000012">
        <property type="entry name" value="Replication factor C subunit 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.60">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.272.10">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.170.16.10">
        <property type="entry name" value="Hedgehog/Intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003959">
        <property type="entry name" value="ATPase_AAA_core"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008921">
        <property type="entry name" value="DNA_pol3_clamp-load_cplx_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050238">
        <property type="entry name" value="DNA_Rep/Repair_Clamp_Loader"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003586">
        <property type="entry name" value="Hint_dom_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003587">
        <property type="entry name" value="Hint_dom_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036844">
        <property type="entry name" value="Hint_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006142">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030934">
        <property type="entry name" value="Intein_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006141">
        <property type="entry name" value="Intein_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013748">
        <property type="entry name" value="Rep_factorC_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR047854">
        <property type="entry name" value="RFC_lid"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008824">
        <property type="entry name" value="RuvB-like_N"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01443">
        <property type="entry name" value="intein_Cterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01445">
        <property type="entry name" value="intein_Nterm"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF001679">
        <property type="entry name" value="PRK00440.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669">
        <property type="entry name" value="REPLICATION FACTOR C / DNA POLYMERASE III GAMMA-TAU SUBUNIT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11669:SF20">
        <property type="entry name" value="REPLICATION FACTOR C SUBUNIT 4"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00004">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25361">
        <property type="entry name" value="AAA_lid_RFC1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF14890">
        <property type="entry name" value="Intein_splicing"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08542">
        <property type="entry name" value="Rep_fac_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05496">
        <property type="entry name" value="RuvB_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00379">
        <property type="entry name" value="INTEIN"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00305">
        <property type="entry name" value="HintC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00306">
        <property type="entry name" value="HintN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51294">
        <property type="entry name" value="Hedgehog/intein (Hint) domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF48019">
        <property type="entry name" value="post-AAA+ oligomerization domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50818">
        <property type="entry name" value="INTEIN_C_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50817">
        <property type="entry name" value="INTEIN_N_TER"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="6" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="5" id="KW-0068">Autocatalytic cleavage</keyword>
    <keyword evidence="3" id="KW-0235">DNA replication</keyword>
    <keyword evidence="4" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="7" id="KW-0651">Protein splicing</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="AAA+ ATPase" evidence="11">
        <location>
            <begin position="35"/>
            <end position="484"/>
        </location>
    </feature>
    <feature type="domain" description="Hint" evidence="10">
        <location>
            <begin position="73"/>
            <end position="193"/>
        </location>
    </feature>
    <feature type="domain" description="Hint" evidence="9">
        <location>
            <begin position="354"/>
            <end position="402"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00009668"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00014164"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022705"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022813"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00023000"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00031749"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="9">
        <source>
            <dbReference type="SMART" id="SM00305"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM00306"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="SMART" id="SM00382"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN96371.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="640" mass="73665" checksum="3196183A37423EC7" modified="2013-06-26" version="1">MEKPWVEKYRPKRLDEIVGQEEIVKRLKKYVEKRSMPHLLFSGPPGVGKTTAALCLARELYGENWRENFLELNASVAKDTPILVKINGVIKRTTFEELDKLYFNSENIEKLFDGEYVETKNLEVLTVENNKVVWKPVKYLIRHKVNKILEVRFEGGKIKLTGNHSVMVFNENGELTTKHAKDLKVGDCIISFVSSLKDKRYELTSMLYAAKGFKNKIRYPNIIKLVYLKGLADVNSCENWDGVVRLSSKDLSVDIVWLSRILGIESSIFKGEVRLMWKKHKNSDLLPAKMFIKFFEKISDKIKINWRCILRHQLYGNKNMVSKNKVIAILNNIKVDELTEDERKIYKNLRNIALSDLHVVKIKEIKLLDYNDYVYDVSVPNNEMFFAGEVPILLHNSDERGIDVIRTKVKEFARTKPIADAPFKIVFLDESDALTADAQNALRRTMEKYSDVCRFILSCNYPSKIIPPIQSRCAVFRFSPLKKEEIAKKLKEIAEKEGLKLEPSGLEAIIYVSEGDMRKAINILQTAAALDNVITDELVYKVSSRARPEEVKEMMNLALEGNFEKARDLLYKLMVEWGMSGEDILNQMFREINNLNIPEKKKVELAEAIGETDFRIVEGANERIQLSALLAKMALLGKRL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="39" xmlns="https://uniprot.org/uniprot">
    <accession>N6VT77</accession>
    <name>N6VT77_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="12">Digeranylgeranylglyceryl phosphate synthase</fullName>
            <shortName evidence="12">DGGGP synthase</shortName>
            <shortName evidence="12">DGGGPS</shortName>
            <ecNumber evidence="12">2.5.1.42</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="12">(S)-2,3-di-O-geranylgeranylglyceryl phosphate synthase</fullName>
        </alternativeName>
        <alternativeName>
            <fullName evidence="12">Geranylgeranylglycerol-phosphate geranylgeranyltransferase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="13" type="ORF">J422_02459</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="12">Prenyltransferase that catalyzes the transfer of the geranylgeranyl moiety of geranylgeranyl diphosphate (GGPP) to the C2 hydroxyl of (S)-3-O-geranylgeranylglyceryl phosphate (GGGP). This reaction is the second ether-bond-formation step in the biosynthesis of archaeal membrane lipids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="12">
            <text>sn-3-O-(geranylgeranyl)glycerol 1-phosphate + (2E,6E,10E)-geranylgeranyl diphosphate = 2,3-bis-O-(geranylgeranyl)-sn-glycerol 1-phosphate + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:18109"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:57677"/>
            <dbReference type="ChEBI" id="CHEBI:58756"/>
            <dbReference type="ChEBI" id="CHEBI:58837"/>
            <dbReference type="EC" id="2.5.1.42"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="12">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="12">Membrane lipid metabolism; glycerophospholipid metabolism.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 12">Cell membrane</location>
            <topology evidence="1 12">Multi-pass membrane protein</topology>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="12">Belongs to the UbiA prenyltransferase family. DGGGP synthase subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.42" evidence="12"/>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96401.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590433.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VT77"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00781"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.481"/>
    <dbReference type="OrthoDB" id="11851at2157"/>
    <dbReference type="UniPathway" id="UPA00940"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005886">
        <property type="term" value="C:plasma membrane"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0047295">
        <property type="term" value="F:geranylgeranylglycerol-phosphate geranylgeranyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046474">
        <property type="term" value="P:glycerophospholipid biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd13961">
        <property type="entry name" value="PT_UbiA_DGGGPS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.357.140">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.20.120.1780">
        <property type="entry name" value="UbiA prenyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01286">
        <property type="entry name" value="DGGGP_synth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023547">
        <property type="entry name" value="DGGGP_synth"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR050475">
        <property type="entry name" value="Prenyltransferase_related"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000537">
        <property type="entry name" value="UbiA_prenyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044878">
        <property type="entry name" value="UbiA_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42723:SF1">
        <property type="entry name" value="CHLOROPHYLL SYNTHASE, CHLOROPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01040">
        <property type="entry name" value="UbiA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="2 12" id="KW-1003">Cell membrane</keyword>
    <keyword evidence="3 12" id="KW-0444">Lipid biosynthesis</keyword>
    <keyword evidence="8 12" id="KW-0443">Lipid metabolism</keyword>
    <keyword evidence="6 12" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9 12" id="KW-0472">Membrane</keyword>
    <keyword evidence="10 12" id="KW-0594">Phospholipid biosynthesis</keyword>
    <keyword evidence="11 12" id="KW-1208">Phospholipid metabolism</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 12" id="KW-0808">Transferase</keyword>
    <keyword evidence="5 12" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="7 12" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="21"/>
            <end position="46"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="86"/>
            <end position="114"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="126"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="150"/>
            <end position="170"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="191"/>
            <end position="215"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="221"/>
            <end position="242"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="12">
        <location>
            <begin position="254"/>
            <end position="271"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004651"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022475"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022516"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022692"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022989"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00023098"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023136"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00023209"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01286"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96401.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="272" mass="30710" checksum="88A177056D8595D9" modified="2013-06-26" version="1">MKAFIELIRMKNCISAGIGGVIGYLLSCNFCFDYKAILIFLTIFFICGYGNVINDIYDIEIDKINKPYRPLPSGRVKIFEAKMLSFSFLIIGIVLSIFINILAFIIAVINSSLLYLYAKTFKKIKIFGNIIVAYLTGSVFLFGAAAGKEVLVAIILFICSFLATWAREIIKDFEDIKGDELKGVVSLPIKYGKISLYFSGFLLILAIVFSFIPYILNIFNIYYLILISACDFLFLISIVKLLRHQNAKEVSKEIKIIMNLVLLAFIISSLIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="15" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTD1</accession>
    <name>N6VTD1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02140</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96451.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTD1"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96451.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="24" mass="2593" checksum="BA3488637A637F45" modified="2013-06-26" version="1">MAIAEAAKDLGITPVENHPFNKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTF2</accession>
    <name>N6VTF2_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Molybdopterin biosynthesis MoaE protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_02215</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000014">
        <property type="protein sequence ID" value="ENN96466.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590270.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000014.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTF2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01191"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.435"/>
    <dbReference type="OrthoDB" id="45235at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006777">
        <property type="term" value="P:Mo-molybdopterin cofactor biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.1170.40">
        <property type="entry name" value="Molybdopterin biosynthesis MoaE subunit"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036563">
        <property type="entry name" value="MoaE_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003448">
        <property type="entry name" value="Mopterin_biosynth_MoaE"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02391">
        <property type="entry name" value="MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54690">
        <property type="entry name" value="Molybdopterin synthase subunit MoaE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96466.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="119" mass="14226" checksum="99B6C8E70ACFC2AC" modified="2013-06-26" version="1">MIVNSYEDFKKELNNMIKKYRGKFGGIIGYDGFVRDYDVIEGKKVPCKEKYIESNIINRLKLIEEEVRKRYDIIDILIYHNTGLIKVGERLAAIRIFAKHRNEAFKALEYFINEMKKYH</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="30" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTH9</accession>
    <name>N6VTH9_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">acylphosphatase</fullName>
            <ecNumber evidence="1">3.6.1.7</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_01655</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="1">
            <text>an acyl phosphate + H2O = a carboxylate + phosphate + H(+)</text>
            <dbReference type="Rhea" id="RHEA:14965"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:29067"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:59918"/>
            <dbReference type="EC" id="3.6.1.7"/>
        </reaction>
    </comment>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.6.1.7" evidence="1"/>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96486.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590091.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTH9"/>
    <dbReference type="OrthoDB" id="6643at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003998">
        <property type="term" value="F:acylphosphatase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016743">
        <property type="term" value="F:carboxyl- or carbamoyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008270">
        <property type="term" value="F:zinc ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051604">
        <property type="term" value="P:protein maturation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.870.50">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001792">
        <property type="entry name" value="Acylphosphatase-like_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036046">
        <property type="entry name" value="Acylphosphatase-like_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017968">
        <property type="entry name" value="Acylphosphatase_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR051060">
        <property type="entry name" value="Carbamoyltrans_HypF-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011125">
        <property type="entry name" value="Znf_HypF"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42959">
        <property type="entry name" value="CARBAMOYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42959:SF1">
        <property type="entry name" value="CARBAMOYLTRANSFERASE HYPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00708">
        <property type="entry name" value="Acylphosphatase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF07503">
        <property type="entry name" value="zf-HYPF"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF54975">
        <property type="entry name" value="Acylphosphatase/BLUF domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00150">
        <property type="entry name" value="ACYLPHOSPHATASE_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51160">
        <property type="entry name" value="ACYLPHOSPHATASE_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Acylphosphatase-like" evidence="2">
        <location>
            <begin position="1"/>
            <end position="85"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="16"/>
        </location>
    </feature>
    <feature type="active site" evidence="1">
        <location>
            <position position="34"/>
        </location>
    </feature>
    <feature type="non-terminal residue" evidence="3">
        <location>
            <position position="142"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00520"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS51160"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96486.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="142" mass="16535" checksum="DF9D2BEC6930565A" modified="2013-06-26" version="1" fragment="single">MIRIIVKGIVQGVGFRPFVYRLAKKYNLKGYVKNCGNYVEIVVKGNNIERFINDLITKKPEIAKIDEIETEEIEDLDIDDFYILKSSNGKEDIGIIPADLSICEDCLREMFNKKDRRFRYPFTACLNCGPKFTIIEKLPYDR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="26" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTR2</accession>
    <name>N6VTR2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">TIGR00341 family protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_02080</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000013">
        <property type="protein sequence ID" value="ENN96556.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590199.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000013.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTR2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00260"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.409"/>
    <dbReference type="OrthoDB" id="3266at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005240">
        <property type="entry name" value="DUF389"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00341">
        <property type="entry name" value="TIGR00341 family protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00271">
        <property type="entry name" value="uncharacterized hydrophobic domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20992">
        <property type="entry name" value="AT15442P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR20992:SF9">
        <property type="entry name" value="AT15442P-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04087">
        <property type="entry name" value="DUF389"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="113"/>
            <end position="129"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="135"/>
            <end position="160"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="172"/>
            <end position="191"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="203"/>
            <end position="223"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="230"/>
            <end position="251"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="257"/>
            <end position="280"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96556.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="286" mass="30821" checksum="6B0F0F366F65854E" modified="2013-06-26" version="1">MRYMKIMVPKKFLNTVEEILKKNNAYSISIIEPLKSSIENGIIITCNAKARDAEKIVLELKKMGLGEKGYGSITIMPANITFSCREEGLASTSLSPLELYYKAKSMVKITKTPIIKVILASIVGTIGLIEHNIPALIGAMIIAPLVDTVMGSAIGTVLGNKELFIEGMKKELLCVGIVTLCSFFMGLFFVSKSLVLEYLSGGSILLSSIIAIISGISGGMGIASGKEYEIIGVTIDVSILIPALLMGIALATMDFKLIYTAFILLILNIILLDIGGYIGLKYKIKN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="29" xmlns="https://uniprot.org/uniprot">
    <accession>N6VTZ1</accession>
    <name>N6VTZ1_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="5">Glycogen synthase GlgA</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="5" type="ORF">J422_01066</name>
    </gene>
    <organism evidence="5 6">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="5 6" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="5 6">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="5">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000007">
        <property type="protein sequence ID" value="ENN96641.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589881.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000007.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VTZ1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00830"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.207"/>
    <dbReference type="OrthoDB" id="132546at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016757">
        <property type="term" value="F:glycosyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2000">
        <property type="entry name" value="Glycogen Phosphorylase B"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001296">
        <property type="entry name" value="Glyco_trans_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013534">
        <property type="entry name" value="Starch_synth_cat_dom"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825:SF11">
        <property type="entry name" value="ALPHA AMYLASE DOMAIN-CONTAINING PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR45825">
        <property type="entry name" value="GRANULE-BOUND STARCH SYNTHASE 1, CHLOROPLASTIC/AMYLOPLASTIC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF08323">
        <property type="entry name" value="Glyco_transf_5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00534">
        <property type="entry name" value="Glycos_transf_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53756">
        <property type="entry name" value="UDP-Glycosyltransferase/glycogen phosphorylase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0328">Glycosyltransferase</keyword>
    <keyword evidence="6" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Starch synthase catalytic" evidence="4">
        <location>
            <begin position="2"/>
            <end position="192"/>
        </location>
    </feature>
    <feature type="domain" description="Glycosyl transferase family 1" evidence="3">
        <location>
            <begin position="290"/>
            <end position="442"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022676"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="Pfam" id="PF00534"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="4">
        <source>
            <dbReference type="Pfam" id="PF08323"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="EMBL" id="ENN96641.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="512" mass="58842" checksum="331CCAAADFD81488" modified="2013-06-26" version="1">MKIAILAPTITPIVSYGGLGDVMRDLPKFLDKDNDVIVLTLNHYGKYYKLPYEIIGNIPIVYKGSKIVFEVLKTKHPETGVEIIAFSNNKVNNLDVWDPLKYEIFSDLVVAYLDENKVDIVSGHDWMCGLAIAKCKDILDLPTTLTIHNEAFKGSLIEYKGEVLTFLELGLKYADAVNTVSPTHAEEIRNIDYIKKYIENKPFHGILNGIDIDEYDPYKIIDRMYRLSNCKLNPKNYAYVAPYSTEDAHETKPKIKYSWFYNGGVFEYVEDWNKYFDKIEVHGEIKGDITTPLIGFVGRATYQKGFKTIFEALPELLEKHDIRFVFLTKGEKDIEEKLKELAKEFYGKVMSIIGYCLPLTSVIFAGSDWIIMPSYWEPCGLVQMEAMSYCTPVIATETGGLKDTIISLNPNPLENPNFNKATGVLFKVPDKYGLIWGIEHALNWSFYRIKEICMFIQYRKICPDHPYDYNSPLPMMMRNCHHHVVKNLSWQNSPSIKKYIGLFGGGIYKHYF</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="28" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU14</accession>
    <name>N6VU14_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">VWFA domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00996</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000006">
        <property type="protein sequence ID" value="ENN96671.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589867.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000006.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU14"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01019"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.194"/>
    <dbReference type="OrthoDB" id="64524at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005829">
        <property type="term" value="C:cytosol"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01462">
        <property type="entry name" value="VWA_YIEM_type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.410">
        <property type="entry name" value="von Willebrand factor, type A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002035">
        <property type="entry name" value="VWF_A"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036465">
        <property type="entry name" value="vWFA_dom_sf"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36846">
        <property type="entry name" value="PROTEIN VIAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR36846:SF1">
        <property type="entry name" value="PROTEIN VIAA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13519">
        <property type="entry name" value="VWA_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00327">
        <property type="entry name" value="VWA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53300">
        <property type="entry name" value="vWA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50234">
        <property type="entry name" value="VWFA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="VWFA" evidence="1">
        <location>
            <begin position="251"/>
            <end position="414"/>
        </location>
    </feature>
    <evidence type="ECO:0000259" key="1">
        <source>
            <dbReference type="PROSITE" id="PS50234"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96671.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="414" mass="48499" checksum="F9DBE3105F1BE3BF" modified="2013-06-26" version="1">MKNVIKHDAFDKKAFERFLNSNKYFQKLLSYYSHLHPIHEKLSEDIFYAFFKYVVEFNEAVEEKFRINKAILKGLIKNIEYEKIRLLTELDETNAGIATVILCEEIFKNFKKFNGSASDLVREILKQNSKNILEIIEGINSFGFGKGEKNYNDPEDKLKLANRILQDKKLLKIIKKLGKLKLLAINTYKNNVKQQLSEVYSVSLGNEIKYLLPKEIAYLSDEILYYEFIRRFIEKKLLNYSLISYKDSEGAIIVCLDHSGSMFGEKEIWAKAVVLSLIEIAKRKKRKIYYIAFDDDVRFEKEIDPKNIKYEDVIEIASVFYGGGTNFEKPISRAMDVIKEKYMDADILLITDGFAEVSDEFVEKLKEFKENYKVKLMSVFIEIFPTETLKRISDKNVKVYELVDDEAKKIYKLL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU39</accession>
    <name>N6VU39_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4">Stage II sporulation protein M</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00641</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96706.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589806.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU39"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00906"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.123"/>
    <dbReference type="OrthoDB" id="65825at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="20"/>
            <end position="42"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="85"/>
            <end position="104"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="116"/>
            <end position="136"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="156"/>
            <end position="174"/>
        </location>
    </feature>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="186"/>
            <end position="210"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96706.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="4">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="237" mass="26962" checksum="916144C5D44482B1" modified="2013-06-26" version="1">MFKKFLCLNLKYKITLTYFLTWLGFLISFSFGKFLIFLSNILKSEPIKKPAEIAKTFGEMKFYAVSSVISQNVSIPYLSYALSYIINNSISCMMIIAVFALAGYLSSKEKAEEKEYLRFICILFIFAIANPITALIGVNLEFKDLIAIVPHGIFEFFGYSLAVVLGLELANIFYPIKEKVSIKSIAILLMSIFTFISIAGFLEPIDWLIYNYAKANNLDLFKTFFTVYKSLIWGLNG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU43</accession>
    <name>N6VU43_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="1">UPF0210 protein J422_00676</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_00676</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1">Belongs to the UPF0210 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96711.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589811.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU43"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00900"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.130"/>
    <dbReference type="OrthoDB" id="21376at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="CDD" id="cd08025">
        <property type="entry name" value="RNR_PFL_like_DUF711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.70.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01221">
        <property type="entry name" value="UPF0210"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007841">
        <property type="entry name" value="UPF0210"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF003700">
        <property type="entry name" value="PRK05313.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37560:SF1">
        <property type="entry name" value="UPF0210 PROTEIN MJ1665"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR37560">
        <property type="entry name" value="UPF0210 PROTEIN SPR0218"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05167">
        <property type="entry name" value="DUF711"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51998">
        <property type="entry name" value="PFL-like glycyl radical enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01221"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96711.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="456" mass="48140" checksum="4416DC7ACBE92FAB" modified="2013-06-26" version="1">MFLPEEIIETIKMIKMENLDLRTVTMGISLLDCVSNDLDELKENIYNKIVSSAENLVETADRISEKYGVPIVNKRVAVTPIALLIGGALKNIDDKIGACVEVGKTLDKAAKKVKVDFLGGYSALVHKDATEADLCLINSIPDMMKMTERVCSSVNVASTRTGINMDIVKLMGKIIKDTAYKTEKAIGCAKLVVFANAPEDNPFMAGAFHGVGEGDKAINVGISGPGVVRAVVEKLKNADFGTLHNEIKKTAFKITRVGELIGREICKELNIKFGIVDLSLAPTPAKGDSVANILEAMGLEKCGTHGSVAALALLNDAVKKGGLMATTYAGGLSGAFIPVSEDSGMVEAVKAGALSLEKLEAMTCVCSVGLDMIAIPGDTPEATISAIIADEMAIGVINNKTTAVRIIPIPGAKEGDIVEYGGLLGKAIVMKVNKFSSEKFINRGGKIPAPLQGFTN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6VU68</accession>
    <name>N6VU68_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">tRNA uridine(34) acetyltransferase</fullName>
            <ecNumber evidence="14">2.3.1.311</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="17">Elongator complex protein 3 homolog</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="20" type="ORF">J422_00856</name>
    </gene>
    <organism evidence="20 21">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="20 21" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="20 21">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="16">tRNA uridine(34) acetyltransferase, which mediates formation of carboxymethyluridine in the wobble base at position 34 in tRNAs. The proposed mechanism is the following: (i) recruits S-adenosyl-L-methionine and cleaves it to generate a 5'-deoxyadenosine radical (5'-dA) in the radical S-adenosyl-L-methionine (rSAM) region, (ii) hydrolyzes acetyl-CoA in the N-acetyltransferase domain and (iii) an acetyl radical is formed by the products of the two domains and (iv) is transferred onto the C5 position of uridine(34) in the bound tRNA molecule. Does not show protein lysine acetyltransferase activity.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="15">
            <text>uridine(34) in tRNA + acetyl-CoA + S-adenosyl-L-methionine + H2O = 5-(carboxymethyl)uridine(34) in tRNA + 5'-deoxyadenosine + L-methionine + CoA + 2 H(+)</text>
            <dbReference type="Rhea" id="RHEA:61020"/>
            <dbReference type="Rhea" id="RHEA-COMP:10407"/>
            <dbReference type="Rhea" id="RHEA-COMP:11727"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17319"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:57844"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:65315"/>
            <dbReference type="ChEBI" id="CHEBI:74882"/>
            <dbReference type="EC" id="2.3.1.311"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="15">
            <dbReference type="Rhea" id="RHEA:61021"/>
        </physiologicalReaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="18">
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
        </cofactor>
        <text evidence="18">Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.</text>
    </comment>
    <comment type="pathway">
        <text evidence="1">tRNA modification.</text>
    </comment>
    <comment type="similarity">
        <text evidence="2">Belongs to the ELP3 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="20">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.3.1.311" evidence="14"/>
    <dbReference type="EMBL" id="APMM01000004">
        <property type="protein sequence ID" value="ENN96741.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589841.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000004.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VU68"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00863"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.169"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051539">
        <property type="term" value="F:4 iron, 4 sulfur cluster binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000049">
        <property type="term" value="F:tRNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0106261">
        <property type="term" value="F:tRNA uridine(34) acetyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0002926">
        <property type="term" value="P:tRNA wobble base 5-methoxycarbonylmethyl-2-thiouridinylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd01335">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000388">
        <property type="entry name" value="Histone acetyltransferase Elp3 homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.630.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016181">
        <property type="entry name" value="Acyl_CoA_acyltransferase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR039661">
        <property type="entry name" value="ELP3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR034687">
        <property type="entry name" value="ELP3-like"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056591">
        <property type="entry name" value="ELP3-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR006638">
        <property type="entry name" value="Elp3/MiaA/NifB-like_rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR032432">
        <property type="entry name" value="Radical_SAM_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR007197">
        <property type="entry name" value="rSAM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058240">
        <property type="entry name" value="rSAM_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR01211">
        <property type="entry name" value="ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135">
        <property type="entry name" value="HISTONE ACETYLTRANSFERASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR11135:SF7">
        <property type="entry name" value="TRNA URIDINE(34) ACETYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF23613">
        <property type="entry name" value="ELP3_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF04055">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF16199">
        <property type="entry name" value="Radical_SAM_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF005669">
        <property type="entry name" value="Hist_AcTrfase_ELP3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDG01086">
        <property type="entry name" value="elongater_protein-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDF00344">
        <property type="entry name" value="ELP3-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SFLD" id="SFLDS00029">
        <property type="entry name" value="Radical_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00729">
        <property type="entry name" value="Elp3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55729">
        <property type="entry name" value="Acyl-CoA N-acyltransferases (Nat)"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF102114">
        <property type="entry name" value="Radical SAM enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51918">
        <property type="entry name" value="RADICAL_SAM"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4" id="KW-0004">4Fe-4S</keyword>
    <keyword evidence="13" id="KW-0012">Acyltransferase</keyword>
    <keyword evidence="11 18" id="KW-0408">Iron</keyword>
    <keyword evidence="12 18" id="KW-0411">Iron-sulfur</keyword>
    <keyword evidence="9 18" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="21" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="10" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="6" id="KW-0808">Transferase</keyword>
    <keyword evidence="8" id="KW-0819">tRNA processing</keyword>
    <keyword evidence="5" id="KW-0820">tRNA-binding</keyword>
    <feature type="domain" description="Radical SAM core" evidence="19">
        <location>
            <begin position="74"/>
            <end position="343"/>
        </location>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="89"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <feature type="binding site" evidence="18">
        <location>
            <position position="97"/>
        </location>
        <ligand>
            <name>[4Fe-4S] cluster</name>
            <dbReference type="ChEBI" id="CHEBI:49883"/>
            <note>4Fe-4S-S-AdoMet</note>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00005217"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00005494"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00018747"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022485"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022555"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="ARBA" id="ARBA00023004"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="12">
        <source>
            <dbReference type="ARBA" id="ARBA00023014"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="13">
        <source>
            <dbReference type="ARBA" id="ARBA00023315"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="14">
        <source>
            <dbReference type="ARBA" id="ARBA00044771"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="15">
        <source>
            <dbReference type="ARBA" id="ARBA00047372"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="16">
        <source>
            <dbReference type="ARBA" id="ARBA00056275"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="17">
        <source>
            <dbReference type="ARBA" id="ARBA00076679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="18">
        <source>
            <dbReference type="PIRSR" id="PIRSR005669-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="19">
        <source>
            <dbReference type="PROSITE" id="PS51918"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="20">
        <source>
            <dbReference type="EMBL" id="ENN96741.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="21">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="535" mass="62130" checksum="C8C40CBAFAFED1E8" modified="2013-06-26" version="1">MREKMIRTLINKLLEAYKRGEKLDKKKIERIKRECLREFRGLSFPSTSEILEYANEEEKRILIPLLRKKPVRSISGVSVVAVMTSPSKCPHGKCIFCPGGVNSCFGDVPQSYTGKEPATMRGLMFNFDPYLQTKARLEQFKKIGHPIDKIELIIMGGTFPARNIEYQDWFIKRCLDALNEKESKSLEEAQKINETAKHRCVALCVETRPDYCKEKDINQMLKLGTTRVEIGVQTIYNEILEFCKRGHTVEDSIKATQLLKDSGLKVSYHIMPGLPGSDIDKDKEMFKEIFENPDFRPDMVKIYPCLVIEGTELYELWKKGEYKPYREEEAIEVIAYAKSIMPKWVRTSRIQRDIPATVIVDGVKKSNLGELVYKYLEEKNIKCRCIRCREVGHVMYKRGIIPDLKHVKLCREEYEASNGVEIFLSFEDVKNDILIAFLRLREPYKPFRKEIDEKTMLVRQLHVCGPEKPLKGEFEELSLQHMGFGRKLLMEAERVAEEEFDKNKILVTSGIGVREYYRKLGYKRIGPYMGKVIKS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="49" xmlns="https://uniprot.org/uniprot">
    <accession>N6VX12</accession>
    <name>N6VX12_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">Cytidylate kinase</fullName>
            <shortName evidence="10">CK</shortName>
            <ecNumber evidence="10">2.7.4.25</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">Cytidine monophosphate kinase</fullName>
            <shortName evidence="10">CMP kinase</shortName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10" type="primary">cmk</name>
        <name evidence="11" type="ORF">J422_06653</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="9 10">
            <text>CMP + ATP = CDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:11600"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:58069"/>
            <dbReference type="ChEBI" id="CHEBI:60377"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="8 10">
            <text>dCMP + ATP = dCDP + ADP</text>
            <dbReference type="Rhea" id="RHEA:25094"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:57566"/>
            <dbReference type="ChEBI" id="CHEBI:58593"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="2.7.4.25"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="1 10">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="2 10">Belongs to the cytidylate kinase family. Type 2 subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.4.25" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000057">
        <property type="protein sequence ID" value="ENN95647.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593896.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000057.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VX12"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00720"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1295"/>
    <dbReference type="OrthoDB" id="31096at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036430">
        <property type="term" value="F:CMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="RHEA"/>
    </dbReference>
    <dbReference type="GO" id="GO:0036431">
        <property type="term" value="F:dCMP kinase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006220">
        <property type="term" value="P:pyrimidine nucleotide metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd02020">
        <property type="entry name" value="CMPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00239">
        <property type="entry name" value="Cytidyl_kinase_type2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011892">
        <property type="entry name" value="Cyt_kin_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011994">
        <property type="entry name" value="Cytidylate_kinase_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02173">
        <property type="entry name" value="cyt_kin_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF13189">
        <property type="entry name" value="Cytidylate_kin2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="6 10 11" id="KW-0418">Kinase</keyword>
    <keyword evidence="5 10" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="4 10 11" id="KW-0808">Transferase</keyword>
    <feature type="binding site" evidence="10">
        <location>
            <begin position="7"/>
            <end position="15"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00011005"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022777"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00047615"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00048478"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00239"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95647.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="177" mass="20610" checksum="138B23F20448D5DE" modified="2013-06-26" version="1">MIITIGGLPGTGTTTIAKKIAEKYHLKYVCAGLIFREMAKEKGMSLEEFSKYAEKHREVDEEIDRRQVELAKQGNVILEGRLAAWMLIRNNIKPDLTIWFKAPIEVRAQRISERENIDYETALKNMIEREKSEKKRYKEIYNIDLDDLSIYDLILDTSKWNVEGVFNIVSEAINNLR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="42" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXC6</accession>
    <name>N6VXC6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="7">tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase</fullName>
            <ecNumber evidence="7">2.5.1.114</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="7">tRNA wyosine derivatives biosynthesis protein Taw2</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="7" type="primary">taw2</name>
        <name evidence="9" type="ORF">J422_05943</name>
    </gene>
    <organism evidence="9 10">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="9 10" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="9 10">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="6 7">S-adenosyl-L-methionine-dependent transferase that acts as a component of the wyosine derivatives biosynthesis pathway. Catalyzes the transfer of the alpha-amino-alpha-carboxypropyl (acp) group from S-adenosyl-L-methionine to 4-demethylwyosine (imG-14), forming 7-aminocarboxypropyl-demethylwyosine (wybutosine-86) at position 37 of tRNA(Phe).</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5 7">
            <text>4-demethylwyosine(37) in tRNA(Phe) + S-adenosyl-L-methionine = 4-demethyl-7-[(3S)-3-amino-3-carboxypropyl]wyosine(37) in tRNA(Phe) + S-methyl-5'-thioadenosine + H(+)</text>
            <dbReference type="Rhea" id="RHEA:36355"/>
            <dbReference type="Rhea" id="RHEA-COMP:10164"/>
            <dbReference type="Rhea" id="RHEA-COMP:10378"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:17509"/>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
            <dbReference type="ChEBI" id="CHEBI:64315"/>
            <dbReference type="ChEBI" id="CHEBI:73550"/>
            <dbReference type="EC" id="2.5.1.114"/>
        </reaction>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="7">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="7">Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.</text>
    </comment>
    <comment type="caution">
        <text evidence="9">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.5.1.114" evidence="7"/>
    <dbReference type="EMBL" id="APMM01000047">
        <property type="protein sequence ID" value="ENN95777.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004593036.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000047.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXC6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00570"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1157"/>
    <dbReference type="OrthoDB" id="8079at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0102522">
        <property type="term" value="F:tRNA 4-demethylwyosine alpha-amino-alpha-carboxypropyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0008175">
        <property type="term" value="F:tRNA methyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030488">
        <property type="term" value="P:tRNA methylation"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="CDD" id="cd02440">
        <property type="entry name" value="AdoMet_MTases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.40.50.150:FF:000431">
        <property type="entry name" value="tRNA(Phe) (4-demethylwyosine(37)-C(7)) aminocarboxypropyltransferase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.150">
        <property type="entry name" value="Vaccinia Virus protein VP39"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01922">
        <property type="entry name" value="TYW2_archaea"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030382">
        <property type="entry name" value="MeTrfase_TRM5/TYW2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029063">
        <property type="entry name" value="SAM-dependent_MTases_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056743">
        <property type="entry name" value="TRM5-TYW2-like_MTfase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR056744">
        <property type="entry name" value="TRM5/TYW2-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR030867">
        <property type="entry name" value="TYW2_archaea"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF047824">
        <property type="entry name" value="tRNAwyosineTaw2Meth"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245">
        <property type="entry name" value="TRNA METHYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR23245:SF41">
        <property type="entry name" value="TRNA(PHE) (4-DEMETHYLWYOSINE(37)-C(7)) AMINOCARBOXYPROPYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02475">
        <property type="entry name" value="TRM5-TYW2_MTfase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF25133">
        <property type="entry name" value="TYW2_N_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53335">
        <property type="entry name" value="S-adenosyl-L-methionine-dependent methyltransferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51684">
        <property type="entry name" value="SAM_MT_TRM5_TYW2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="1 7" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="10" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="3 7" id="KW-0949">S-adenosyl-L-methionine</keyword>
    <keyword evidence="2 7" id="KW-0808">Transferase</keyword>
    <keyword evidence="4 7" id="KW-0819">tRNA processing</keyword>
    <feature type="domain" description="SAM-dependent methyltransferase TRM5/TYW2-type" evidence="8">
        <location>
            <begin position="3"/>
            <end position="246"/>
        </location>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="78"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="85"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="125"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <begin position="152"/>
            <end position="153"/>
        </location>
        <ligand>
            <name>S-adenosyl-L-methionine</name>
            <dbReference type="ChEBI" id="CHEBI:59789"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022691"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022694"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00049400"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00058518"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01922"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="8">
        <source>
            <dbReference type="PROSITE" id="PS51684"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="9">
        <source>
            <dbReference type="EMBL" id="ENN95777.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="10">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="248" mass="29165" checksum="E5F73EE4AE3D3CD9" modified="2013-06-26" version="1">MKYQKIGDIVLVKKPLSEEEIREIVKKTKCKTVALKTSQIVGEFRRPYIKILYGKETETIHKEHGCLFKLDVSKIMWSQGNIKERERMARISNENEIVVDMFAGIGYFSIPLAKYSNPKTVYAIEKNPIAYHYLCENIKLNKLKNIIPILSDNRNVNLRNIADRIIMGYIHKTHKFLPKAFEILKDKGVIHYHETVAEKVMYERPIERLKYYAKIYNYKLVDYKIFKIKKYAPGVWHVVVDAKFCACI</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="31" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXH8</accession>
    <name>N6VXH8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Flagellin</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05868</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000044">
        <property type="protein sequence ID" value="ENN95837.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592848.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000044.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXH8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01233"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1144"/>
    <dbReference type="OrthoDB" id="102632at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005198">
        <property type="term" value="F:structural molecule activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0097588">
        <property type="term" value="P:archaeal or bacterial-type flagellum-dependent cell motility"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002774">
        <property type="entry name" value="Flagellin_arc-type"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR35903:SF1">
        <property type="entry name" value="FLAGELLIN B1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01917">
        <property type="entry name" value="Flagellin_arch-type"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0966">Cell projection</keyword>
    <keyword evidence="1" id="KW-0969">Cilium</keyword>
    <keyword evidence="1" id="KW-0282">Flagellum</keyword>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <feature type="non-terminal residue" evidence="1">
        <location>
            <position position="1"/>
        </location>
    </feature>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95837.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="169" mass="17840" checksum="721689C9642BC949" modified="2013-06-26" version="1" fragment="single">QVASGLQVIRVLGNHSGGKINWLAVLISPNAGSAPIDLSQATVMITDGTHKVIAKYNSTFFNGTLKNGGSIFEAKYNNTTALKPLFDDLPATAFGIVVLQDADTSCSKDTPVINKGDIVAICLNVSNTLNLKPRTKVTGAVIPEFGAPAVISFTTPATYLDTQHIIELQ</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="23" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXL8</accession>
    <name>N6VXL8_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="1">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_05419</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000039">
        <property type="protein sequence ID" value="ENN95882.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592476.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000039.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXL8"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00841"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1058"/>
    <dbReference type="OrthoDB" id="8628at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN95882.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="174" mass="20370" checksum="BAABF8F41B6D2FD0" modified="2013-06-26" version="1">MRVGITKMYKDIADLIGLEEYEIVDPYNKNVNCDFLIISKGYKERVKMFNKNAYIFEVRSATFKDLIESLNELKKLGLGKRDKIDESIEMLKKKERDIKSLINFKFKVNPKTEFIKRVVEDLGLEISEDGVTIAPDYVGEADIIIETHKNLPLIERIEDRYLQIINKIKRLSHN</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="44" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXN6</accession>
    <name>N6VXN6_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="12">Malate dehydrogenase</fullName>
            <ecNumber evidence="12">1.1.1.37</ecNumber>
        </submittedName>
    </protein>
    <gene>
        <name evidence="12" type="ORF">J422_05339</name>
    </gene>
    <organism evidence="12 13">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="12 13" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="12 13">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="similarity">
        <text evidence="1 9">Belongs to the LDH/MDH superfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="12">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="1.1.1.37" evidence="12"/>
    <dbReference type="EMBL" id="APMM01000037">
        <property type="protein sequence ID" value="ENN95902.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592381.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000037.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXN6"/>
    <dbReference type="SMR" id="N6VXN6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01319"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.1042"/>
    <dbReference type="OrthoDB" id="2596at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004459">
        <property type="term" value="F:L-lactate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0030060">
        <property type="term" value="F:L-malate dehydrogenase (NAD+) activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006089">
        <property type="term" value="P:lactate metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006099">
        <property type="term" value="P:tricarboxylic acid cycle"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.110.10">
        <property type="entry name" value="Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.720">
        <property type="entry name" value="NAD(P)-binding Rossmann-like Domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001557">
        <property type="entry name" value="L-lactate/malate_DH"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR022383">
        <property type="entry name" value="Lactate/malate_DH_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001236">
        <property type="entry name" value="Lactate/malate_DH_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR015955">
        <property type="entry name" value="Lactate_DH/Glyco_Ohase_4_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036291">
        <property type="entry name" value="NAD(P)-bd_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF004863">
        <property type="entry name" value="PRK06223.1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128">
        <property type="entry name" value="L-2-HYDROXYCARBOXYLATE DEHYDROGENASE (NAD(P)(+))"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43128:SF16">
        <property type="entry name" value="L-LACTATE DEHYDROGENASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF02866">
        <property type="entry name" value="Ldh_1_C"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00056">
        <property type="entry name" value="Ldh_1_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF000102">
        <property type="entry name" value="Lac_mal_DH"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PRINTS" id="PR00086">
        <property type="entry name" value="LLDHDRGNASE"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56327">
        <property type="entry name" value="LDH C-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51735">
        <property type="entry name" value="NAD(P)-binding Rossmann-fold domains"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5 8" id="KW-0520">NAD</keyword>
    <keyword evidence="3" id="KW-0521">NADP</keyword>
    <keyword evidence="4 9 12" id="KW-0560">Oxidoreductase</keyword>
    <keyword evidence="13" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2" id="KW-0816">Tricarboxylic acid cycle</keyword>
    <feature type="domain" description="Lactate/malate dehydrogenase N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="145"/>
        </location>
    </feature>
    <feature type="domain" description="Lactate/malate dehydrogenase C-terminal" evidence="11">
        <location>
            <begin position="148"/>
            <end position="310"/>
        </location>
    </feature>
    <feature type="active site" description="Proton acceptor" evidence="6">
        <location>
            <position position="178"/>
        </location>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="7"/>
            <end position="13"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="86"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="92"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <position position="99"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="8">
        <location>
            <begin position="121"/>
            <end position="123"/>
        </location>
        <ligand>
            <name>NAD(+)</name>
            <dbReference type="ChEBI" id="CHEBI:57540"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="123"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <feature type="binding site" evidence="7">
        <location>
            <position position="154"/>
        </location>
        <ligand>
            <name>substrate</name>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00008104"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022532"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022857"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023002"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00023027"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-2"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="PIRSR" id="PIRSR000102-3"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="RuleBase" id="RU003369"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="Pfam" id="PF00056"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="11">
        <source>
            <dbReference type="Pfam" id="PF02866"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="EMBL" id="ENN95902.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="312" mass="34376" checksum="5CD863CA2EE24076" modified="2013-06-26" version="1">MKISIIGASGRVGGTTAMLLAKEPYMKDLVLIGREKSLNKLNGLKADIYDALAGARSDANIYVSDDNNLSILDGSDIVIITAGAPREDGMSRLDLAKINAKIVGKYARKISEICDTMLFIITNPVDVMTYKAYVSSNFIREHVFGLGTHLDSLRFKVAIAKFFKVHIDEVRTRIIGEHGETMVPLISATSIGGIPIDKFKEFKNLPLEKIIEDVKTKGDKIIRLKGGSEFGPASAIVNVVRCIANNEKRLLTLSTYLDGEFGLKDLCIGVPVKIGKNGIEEIVDIEMSKEEYEAFKRSAEIIKKYCEEVKDL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="50" xmlns="https://uniprot.org/uniprot">
    <accession>N6VXX4</accession>
    <name>N6VXX4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="9">Protein pelota homolog</fullName>
            <ecNumber evidence="9">3.1.-.-</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="9" type="primary">pelA</name>
        <name evidence="11" type="ORF">J422_05075</name>
    </gene>
    <organism evidence="11 12">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="11 12" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="11 12">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="9">May function in recognizing stalled ribosomes, interact with stem-loop structures in stalled mRNA molecules, and effect endonucleolytic cleavage of the mRNA. May play a role in the release non-functional ribosomes and degradation of damaged mRNAs. Has endoribonuclease activity.</text>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1 9">
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </cofactor>
    </comment>
    <comment type="subunit">
        <text evidence="9">Monomer.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="2 9">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="domain">
        <text evidence="9">The N-terminal domain has the RNA-binding Sm fold. It harbors the endoribonuclease activity.</text>
    </comment>
    <comment type="similarity">
        <text evidence="3 9">Belongs to the eukaryotic release factor 1 family. Pelota subfamily.</text>
    </comment>
    <comment type="caution">
        <text evidence="11">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.-.-" evidence="9"/>
    <dbReference type="EMBL" id="APMM01000031">
        <property type="protein sequence ID" value="ENN95987.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004592146.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000031.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VXX4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00474"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.995"/>
    <dbReference type="OrthoDB" id="31300at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004519">
        <property type="term" value="F:endonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016787">
        <property type="term" value="F:hydrolase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0051301">
        <property type="term" value="P:cell division"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070651">
        <property type="term" value="P:nonfunctional rRNA decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070966">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, no-go decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0070481">
        <property type="term" value="P:nuclear-transcribed mRNA catabolic process, non-stop decay"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032790">
        <property type="term" value="P:ribosome disassembly"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0071025">
        <property type="term" value="P:RNA surveillance"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="FunFam" id="2.30.30.870:FF:000002">
        <property type="entry name" value="Protein pelota homolog"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1330.30">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.60">
        <property type="entry name" value="eRF1 domain 2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.30.30.870">
        <property type="entry name" value="Pelota, domain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01853">
        <property type="entry name" value="PelO"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR042226">
        <property type="entry name" value="eFR1_2_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005141">
        <property type="entry name" value="eRF1_2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005142">
        <property type="entry name" value="eRF1_3"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005140">
        <property type="entry name" value="eRF1_Pelota-like_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038069">
        <property type="entry name" value="Pelota/DOM34_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023521">
        <property type="entry name" value="Pelota_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR058547">
        <property type="entry name" value="Pelota_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029064">
        <property type="entry name" value="Ribosomal_eL30-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004405">
        <property type="entry name" value="TF_pelota"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00111">
        <property type="entry name" value="pelota"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853">
        <property type="entry name" value="PELOTA"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10853:SF0">
        <property type="entry name" value="PROTEIN PELOTA HOMOLOG"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03464">
        <property type="entry name" value="eRF1_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03465">
        <property type="entry name" value="eRF1_3"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF26356">
        <property type="entry name" value="Pelota_N"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01194">
        <property type="entry name" value="eRF1_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF159065">
        <property type="entry name" value="Dom34/Pelota N-terminal domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF55315">
        <property type="entry name" value="L30e-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53137">
        <property type="entry name" value="Translational machinery components"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="11" id="KW-0131">Cell cycle</keyword>
    <keyword evidence="11" id="KW-0132">Cell division</keyword>
    <keyword evidence="4 9" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="7 9" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="8 9" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="6 9" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 9" id="KW-0540">Nuclease</keyword>
    <keyword evidence="12" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="eRF1/Pelota-like N-terminal" evidence="10">
        <location>
            <begin position="1"/>
            <end position="125"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001968"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00009504"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01853"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="10">
        <source>
            <dbReference type="SMART" id="SM01194"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="11">
        <source>
            <dbReference type="EMBL" id="ENN95987.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="12">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="344" mass="39436" checksum="6D6158CD3C587992" modified="2013-06-26" version="1">MKIVEEEKDYIKLMPENLDDLWVLYNVIDEGDVIFALTERRVQDKGDMIRADRGTKRKMILGIEVKNLEFDENTKRLRILGVIIHGPDDIPLGAHHTIEVKPFDEITIKKQWKKWQIEKIKEAIESSKRPKVLVVIMDDESAEVYEVRDYCVKEICSIKSHASKRLDYKINEELKKEYYHEIAKVLKEYEVDNIIVAGPGFAKNSFYNFLSEQYPELKRKVVVESVSTTSKAGLNEVFKRGIINRVYAESRVAKETQLIEKLLEEIAKNGLAVYGIDDVKRAIDYAAIDILLVADDMVRDKRIEEIIDLTENIGGKVVIVSTEHDAGKQLKALGGVAGLLRFKV</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYH4</accession>
    <name>N6VYH4_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">YkgJ family cysteine cluster protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03928</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000024">
        <property type="protein sequence ID" value="ENN96172.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591392.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000024.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYH4"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00415"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.770"/>
    <dbReference type="OrthoDB" id="36424at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005358">
        <property type="entry name" value="Puta_zinc/iron-chelating_dom"/>
    </dbReference>
    <dbReference type="Pfam" id="PF03692">
        <property type="entry name" value="CxxCxxCC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96172.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="246" mass="30101" checksum="75EC4F7318396796" modified="2013-06-26" version="1">MRCAYCCSCKGWRIYLNYFDLIKLKDYNYAIERCEGFFNYRLKVNEKGCILLKDNLCRVHIEKGLEYKPLMCLIFPFSCMVKWDGTPLLIIKHYCNGIKKGKIDKKIVKKAIEYIKELYFDKFDNFLKENMEYSSKTYFDEKNRITWEYREELGRYLFNSKNFDELGEKIKEIFNIDINLKRHHDEEEIIRYLLELNRREHFRKLTFKDEVNILLKIANYLTKFKNVLEGEKEIDKMLFIYQKLYL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="21" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYL9</accession>
    <name>N6VYL9_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="2">Uncharacterized protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="2" type="ORF">J422_03458</name>
    </gene>
    <organism evidence="2 3">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="2 3" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="2 3">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="2">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000022">
        <property type="protein sequence ID" value="ENN96222.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004591135.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000022.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYL9"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0472">Membrane</keyword>
    <keyword evidence="3" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1" id="KW-0812">Transmembrane</keyword>
    <keyword evidence="1" id="KW-1133">Transmembrane helix</keyword>
    <feature type="transmembrane region" description="Helical" evidence="1">
        <location>
            <begin position="12"/>
            <end position="32"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="SAM" id="Phobius"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="EMBL" id="ENN96222.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="53" mass="6458" checksum="A0F2EE8B8B8F1DC9" modified="2013-06-26" version="1">MEDKISEYREIVLQTFLMLFIIYVFILIYKHYNPYALPGVEKRFLYLLIILGL</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYP7</accession>
    <name>N6VYP7_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="3">Response regulator receiver protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="3" type="ORF">J422_03278</name>
    </gene>
    <organism evidence="3 4">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="3 4" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="3 4">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="3">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96252.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590952.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000020.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYP7"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01043"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.642"/>
    <dbReference type="OrthoDB" id="2830at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000160">
        <property type="term" value="P:phosphorelay signal transduction system"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.2300">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011006">
        <property type="entry name" value="CheY-like_superfamily"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001789">
        <property type="entry name" value="Sig_transdc_resp-reg_receiver"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR052048">
        <property type="entry name" value="ST_Response_Regulator"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR43228:SF1">
        <property type="entry name" value="TWO-COMPONENT RESPONSE REGULATOR ARR22"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00072">
        <property type="entry name" value="Response_reg"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM00448">
        <property type="entry name" value="REC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52172">
        <property type="entry name" value="CheY-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50110">
        <property type="entry name" value="RESPONSE_REGULATORY"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="1" id="KW-0597">Phosphoprotein</keyword>
    <keyword evidence="4" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="Response regulatory" evidence="2">
        <location>
            <begin position="6"/>
            <end position="122"/>
        </location>
    </feature>
    <feature type="modified residue" description="4-aspartylphosphate" evidence="1">
        <location>
            <position position="57"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00169"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="2">
        <source>
            <dbReference type="PROSITE" id="PS50110"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="3">
        <source>
            <dbReference type="EMBL" id="ENN96252.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="124" mass="13761" checksum="0EE9C361EA539EC4" modified="2013-06-26" version="1">MSKVVKVLVVDDSAFMRNILKRILTQTGKFVVVGEASNGKEAIEKAKELQPDLITMDIVMPEMDGITATREIKKILPNVKIVMCTSVDQEKKVIEALEAGADGYIVKPFQAQKVIDELNKLFPD</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="38" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYQ2</accession>
    <name>N6VYQ2_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="11">Ribonuclease</fullName>
            <ecNumber evidence="11">3.1.26.4</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="13" type="primary">rnhB</name>
        <name evidence="13" type="ORF">J422_03313</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="3 11">Endonuclease that specifically degrades the RNA of RNA-DNA hybrids.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="1 10 11">
            <text>Endonucleolytic cleavage to 5'-phosphomonoester.</text>
            <dbReference type="EC" id="3.1.26.4"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="10">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
        <cofactor evidence="10">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
        <text evidence="10">Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding.</text>
    </comment>
    <comment type="subcellular location">
        <subcellularLocation>
            <location evidence="4">Cytoplasm</location>
        </subcellularLocation>
    </comment>
    <comment type="similarity">
        <text evidence="11">Belongs to the RNase HII family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="3.1.26.4" evidence="11"/>
    <dbReference type="EMBL" id="APMM01000020">
        <property type="protein sequence ID" value="ENN96257.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYQ2"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01049"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.649"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005737">
        <property type="term" value="C:cytoplasm"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-SubCell"/>
    </dbReference>
    <dbReference type="GO" id="GO:0032299">
        <property type="term" value="C:ribonuclease H2 complex"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0046872">
        <property type="term" value="F:metal ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004523">
        <property type="term" value="F:RNA-DNA hybrid ribonuclease activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043137">
        <property type="term" value="P:DNA replication, removal of RNA primer"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006298">
        <property type="term" value="P:mismatch repair"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.420.10">
        <property type="entry name" value="Ribonuclease H-like superfamily/Ribonuclease H"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR004649">
        <property type="entry name" value="RNase_H2_suA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR001352">
        <property type="entry name" value="RNase_HII/HIII"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR024567">
        <property type="entry name" value="RNase_HII/HIII_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR012337">
        <property type="entry name" value="RNaseH-like_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036397">
        <property type="entry name" value="RNaseH_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00729">
        <property type="entry name" value="ribonuclease HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954:SF23">
        <property type="entry name" value="RIBONUCLEASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR10954">
        <property type="entry name" value="RIBONUCLEASE H2 SUBUNIT A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01351">
        <property type="entry name" value="RNase_HII"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53098">
        <property type="entry name" value="Ribonuclease H-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51975">
        <property type="entry name" value="RNASE_H_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="5" id="KW-0963">Cytoplasm</keyword>
    <keyword evidence="8 10 11" id="KW-0255">Endonuclease</keyword>
    <keyword evidence="9 10 11" id="KW-0378">Hydrolase</keyword>
    <keyword evidence="7 10" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="6 10 11" id="KW-0540">Nuclease</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="RNase H type-2" evidence="12">
        <location>
            <begin position="1"/>
            <end position="144"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="6"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="7"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="104"/>
        </location>
        <ligand>
            <name>a divalent metal cation</name>
            <dbReference type="ChEBI" id="CHEBI:60240"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00000077"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00004065"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00004496"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022490"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022722"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022759"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00022801"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU01319"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="RuleBase" id="RU003515"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS51975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96257.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="144" mass="16639" checksum="F2E17C2E0195F21C" modified="2013-06-26" version="1">MILGIDEAGRGCVIGPLVICGYATDEEKLKNIGVKDSKKLNKKERERLKNILESIGYYDVIIITPEELNKMMNKKNLNDIEIEAFKEIIKKFINKFEIEKVYIDACSSNLLSFKRKFKDLDIEIIAEHKADDKYLRCLSSLYNS</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="33" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYW0</accession>
    <name>N6VYW0_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="6">Trans-homoaconitate synthase</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="6" type="primary">aksA</name>
        <name evidence="6" type="ORF">J422_03119</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="catalytic activity">
        <reaction evidence="3">
            <text>acetyl-CoA + 2-oxoglutarate + H2O = (2R)-homocitrate + CoA + H(+)</text>
            <dbReference type="Rhea" id="RHEA:12929"/>
            <dbReference type="ChEBI" id="CHEBI:15377"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:16810"/>
            <dbReference type="ChEBI" id="CHEBI:57287"/>
            <dbReference type="ChEBI" id="CHEBI:57288"/>
            <dbReference type="ChEBI" id="CHEBI:58884"/>
            <dbReference type="EC" id="2.3.3.14"/>
        </reaction>
        <physiologicalReaction direction="left-to-right" evidence="3">
            <dbReference type="Rhea" id="RHEA:12930"/>
        </physiologicalReaction>
    </comment>
    <comment type="similarity">
        <text evidence="1 4">Belongs to the alpha-IPM synthase/homocitrate synthase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96317.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590776.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYW0"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00170"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.612"/>
    <dbReference type="OrthoDB" id="6555at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0004410">
        <property type="term" value="F:homocitrate synthase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019752">
        <property type="term" value="P:carboxylic acid metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="CDD" id="cd07940">
        <property type="entry name" value="DRE_TIM_IPMS"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="1.10.238.260:FF:000001">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="FunFam" id="3.20.20.70:FF:000010">
        <property type="entry name" value="2-isopropylmalate synthase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.238.260">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.70">
        <property type="entry name" value="Aldolase class I"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002034">
        <property type="entry name" value="AIPM/Hcit_synth_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013785">
        <property type="entry name" value="Aldolase_TIM"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011830">
        <property type="entry name" value="LEU1_arch"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR054691">
        <property type="entry name" value="LeuA/HCS_post-cat"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR000891">
        <property type="entry name" value="PYR_CT"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR02090">
        <property type="entry name" value="LEU1_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42880">
        <property type="entry name" value="HOMOCITRATE SYNTHASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42880:SF1">
        <property type="entry name" value="ISOPROPYLMALATE_HOMOCITRATE_CITRAMALATE SYNTHASE FAMILY PROTEIN"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF22617">
        <property type="entry name" value="HCS_D2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00682">
        <property type="entry name" value="HMGL-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51569">
        <property type="entry name" value="Aldolase"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00815">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_1"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00816">
        <property type="entry name" value="AIPM_HOMOCIT_SYNTH_2"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50991">
        <property type="entry name" value="PYR_CT"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0808">Transferase</keyword>
    <feature type="domain" description="Pyruvate carboxyltransferase" evidence="5">
        <location>
            <begin position="24"/>
            <end position="276"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00006154"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00048363"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="RuleBase" id="RU003523"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="5">
        <source>
            <dbReference type="PROSITE" id="PS50991"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96317.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="392" mass="43877" checksum="6B3297AADDE86C1D" modified="2013-06-26" version="1">MDFLFENDWKAPCPYNPKLCLKDIIIYDTTLRDGEQTPGVCFTKDQKLEIAIKLDELGVDQIEAGFPRVSEKEKEAVKAIANQGLKADILALCRTRKEDIDAAIDCDVDGIITFVATSPLHLKYKFGGKSLDEIIKMATEAIEYAKDHGLFVAFSAEDGTRTRIEDLIKIHKKAEEYKADRVHIADTTGSSTPQAMYFMVNMLKKYLNIDIGVHCHNDFGFAVINSIYGLMAGAKAVSTTVNGIGERAGNTALEELIMSLIVLYDVKLDLNLEVLPELCRLVEKYSGISLPKNKPIIGELVFSHESGIHVDAVINNPLTYEPFLPEKIGLKRNIILGKHSGRRAVEYKLKLMGINATEEQICEIVKRVKETRERGIEVSDEVFKKIVEEVIR</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6VYX1</accession>
    <name>N6VYX1_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">DUF749 domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_03169</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000018">
        <property type="protein sequence ID" value="ENN96327.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590796.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000018.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VYX1"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00180"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.622"/>
    <dbReference type="OrthoDB" id="80045at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.160.120">
        <property type="entry name" value="Hypothetical protein MTH1880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR008032">
        <property type="entry name" value="DUF749"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR035933">
        <property type="entry name" value="MTH1880"/>
    </dbReference>
    <dbReference type="Pfam" id="PF05370">
        <property type="entry name" value="DUF749"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF75412">
        <property type="entry name" value="Hypothetical protein MTH1880"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96327.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="90" mass="10372" checksum="8CEC9EE7115501BD" modified="2013-06-26" version="1">MFVAKLIAILSVKEAINSGYGDFVKVRAAIENREMKDDDIVAIFNIDNTTCYHVEFLESLEKIKENLSKINVTLNYDSELIIKRYLEGRK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="40" xmlns="https://uniprot.org/uniprot">
    <accession>N6VZ47</accession>
    <name>N6VZ47_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="4 5">Nascent polypeptide-associated complex protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="4 7" type="primary">nac</name>
        <name evidence="7" type="ORF">J422_02464</name>
    </gene>
    <organism evidence="7 8">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="7 8" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="7 8">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="4">Contacts the emerging nascent chain on the ribosome.</text>
    </comment>
    <comment type="subunit">
        <text evidence="4">Homodimer. Interacts with the ribosome. Binds ribosomal RNA.</text>
    </comment>
    <comment type="similarity">
        <text evidence="4">Belongs to the NAC-alpha family.</text>
    </comment>
    <comment type="caution">
        <text evidence="7">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000016">
        <property type="protein sequence ID" value="ENN96402.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004590435.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000016.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6VZ47"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00780"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.482"/>
    <dbReference type="OrthoDB" id="53273at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0003723">
        <property type="term" value="F:RNA binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0015031">
        <property type="term" value="P:protein transport"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="CDD" id="cd14359">
        <property type="entry name" value="UBA_AeNAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="1.10.8.10">
        <property type="entry name" value="DNA helicase RuvA subunit, C-terminal domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="2.20.70.30">
        <property type="entry name" value="Nascent polypeptide-associated complex domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_00814">
        <property type="entry name" value="NAC_arch"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR044034">
        <property type="entry name" value="NAC-like_UBA"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR038187">
        <property type="entry name" value="NAC_A/B_dom_sf"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR005231">
        <property type="entry name" value="NAC_arc"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002715">
        <property type="entry name" value="Nas_poly-pep-assoc_cplx_dom"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009060">
        <property type="entry name" value="UBA-like_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR00264">
        <property type="entry name" value="archaeal-type nascent polypeptide-associated complex protein"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01849">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF19026">
        <property type="entry name" value="UBA_HYPK"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SMART" id="SM01407">
        <property type="entry name" value="NAC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF46934">
        <property type="entry name" value="UBA-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS51151">
        <property type="entry name" value="NAC_AB"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="3 4" id="KW-0653">Protein transport</keyword>
    <keyword evidence="8" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="2 4" id="KW-0694">RNA-binding</keyword>
    <keyword evidence="1 4" id="KW-0813">Transport</keyword>
    <feature type="domain" description="NAC-A/B" evidence="6">
        <location>
            <begin position="8"/>
            <end position="75"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022448"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022884"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022927"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_00814"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="NCBIfam" id="TIGR00264"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="6">
        <source>
            <dbReference type="PROSITE" id="PS51151"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="EMBL" id="ENN96402.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="8">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="117" mass="13600" checksum="366015471564755E" modified="2013-06-26" version="1">MFPGKINPRMLKQMQKMMKDFGMDAKDLNVRKVIFIFDDEEWHFDEPKVQVMDVMGIKTYSLTGKPKKVKRDVKVEITEDDIKLVAEQCNVSYEEAKKALEECNGDIAEAILKLENK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="24" xmlns="https://uniprot.org/uniprot">
    <accession>N6W061</accession>
    <name>N6W061_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="3">Xylose isomerase-like TIM barrel domain-containing protein</fullName>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="1" type="ORF">J422_00481</name>
    </gene>
    <organism evidence="1 2">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="1 2" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="1 2">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="1">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000003">
        <property type="protein sequence ID" value="ENN96747.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589778.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000003.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W061"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00048"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.92"/>
    <dbReference type="OrthoDB" id="64756at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.20.20.150">
        <property type="entry name" value="Divalent-metal-dependent TIM barrel enzymes"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR036237">
        <property type="entry name" value="Xyl_isomerase-like_sf"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF51658">
        <property type="entry name" value="Xylose isomerase-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-1185">Reference proteome</keyword>
    <evidence type="ECO:0000313" key="1">
        <source>
            <dbReference type="EMBL" id="ENN96747.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="2">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <evidence type="ECO:0008006" key="3">
        <source>
            <dbReference type="Google" id="ProtNLM"/>
        </source>
    </evidence>
    <sequence length="304" mass="34970" checksum="36128A9A7BCF09FF" modified="2013-06-26" version="1">MILFEWGTYNALSTLKQASLLGTRVTEIPPAILSRRLPEGYYESYKRLAKEYFSTILAHGPYYQLSSESGLRGHISAIEKASICGAEIYNYHLGKRVGDDINFHLEVLKKFNEVNNSLIYSPEPATNIGEFGSLDELLELVKAAKDEGIKVIPSLQLENIFLNELKVYETDDLEEASKKADIDWWLKILYKVDKISEYIMHFRFSQVIGLKYKNRFFKKRVPLGKGYPPLEPLVEALSTYLVDNALKGAFKKVLFVYTGLPEVKYKDLIDIYAMIMKRAVDKLMSKEAQLEYGEFYKILNIEEE</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="35" xmlns="https://uniprot.org/uniprot">
    <accession>N6W082</accession>
    <name>N6W082_9EURY</name>
    <protein>
        <submittedName>
            <fullName evidence="4">ABC transporter releated protein</fullName>
        </submittedName>
    </protein>
    <gene>
        <name evidence="4" type="ORF">J422_00381</name>
    </gene>
    <organism evidence="4 5">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="4 5" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="4 5">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="caution">
        <text evidence="4">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EMBL" id="APMM01000002">
        <property type="protein sequence ID" value="ENN96772.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589758.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000002.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W082"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_00704"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.73"/>
    <dbReference type="OrthoDB" id="97750at2157"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016887">
        <property type="term" value="F:ATP hydrolysis activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0019700">
        <property type="term" value="P:organic phosphonate catabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="TreeGrafter"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.300">
        <property type="entry name" value="P-loop containing nucleotide triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003593">
        <property type="entry name" value="AAA+_ATPase"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR003439">
        <property type="entry name" value="ABC_transporter-like_ATP-bd"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR017871">
        <property type="entry name" value="ABC_transporter-like_CS"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR027417">
        <property type="entry name" value="P-loop_NTPase"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42764">
        <property type="entry name" value="PHOSPHONATES UTILIZATION ATP-BINDING PROTEIN PHNK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR42764:SF1">
        <property type="entry name" value="PHOSPHONATES UTILIZATION ATP-BINDING PROTEIN PHNK-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF00005">
        <property type="entry name" value="ABC_tran"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SMART" id="SM00382">
        <property type="entry name" value="AAA"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52540">
        <property type="entry name" value="P-loop containing nucleoside triphosphate hydrolases"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS00211">
        <property type="entry name" value="ABC_TRANSPORTER_1"/>
        <property type="match status" value="2"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50893">
        <property type="entry name" value="ABC_TRANSPORTER_2"/>
        <property type="match status" value="2"/>
    </dbReference>
    <proteinExistence type="predicted"/>
    <keyword evidence="2" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="1" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="5" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="4"/>
            <end position="244"/>
        </location>
    </feature>
    <feature type="domain" description="ABC transporter" evidence="3">
        <location>
            <begin position="255"/>
            <end position="547"/>
        </location>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="3">
        <source>
            <dbReference type="PROSITE" id="PS50893"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="4">
        <source>
            <dbReference type="EMBL" id="ENN96772.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="5">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="548" mass="62511" checksum="9FAD1CAB3D0BE72F" modified="2013-06-26" version="1">MEAVKVKNLVKKYGKKVALDNVSFSVKKGEIFGIVGRSGAGKTTLIKILRGVLDYDKGEIEILGRKDNFREITAIHLQRNFALWAEPVINNVIRKLYAIKNSADESLPMEEEWEEYEREALEILKLVGLEHKKEAFANILSGGEKQRLILARQIAKLYLKKEGVLLLDEPATMACPASKQMLLDAIKNIREKLNITVILTSHLPEIHKYLCDRCLLLEEGKVKMIDEPETVLEEFLKDMKKPYIRKPNIKERNIIEVRNVYKRYFVVHGGETLNLKDVSFDVKEGEIVSIIGPSGVGKTVMIRLLAGLEMPNKGKIIVDGVDMTNYGWERVELRKRIGVMHQEFSLPYYQTVENLLKYRLGVKGEKAIAHAKEKAKELNLSEKIVDAIYQLIDLPEPEKKAKLEKLGLSEDIIYLLFPPIVEDFNPKEILEALDLKEDILKKKVSELSGGEKVRVAMALQLITKPKILFLDEPFGDLDPITLRDVANYLKKINEKFGTTIVLVSHHVDFIKEISDRAILLDKDKIVMNGDPKEVCDEFIKRSEAKFLK</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2026-01-28" version="45" xmlns="https://uniprot.org/uniprot">
    <accession>N6W099</accession>
    <name>N6W099_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="5">2-phospho-L-lactate guanylyltransferase</fullName>
            <shortName evidence="5">LP guanylyltransferase</shortName>
            <ecNumber evidence="5">2.7.7.68</ecNumber>
        </recommendedName>
    </protein>
    <gene>
        <name evidence="5" type="primary">cofC</name>
        <name evidence="6" type="ORF">J422_00025</name>
    </gene>
    <organism evidence="6 7">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="6 7" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="6 7">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="5">Guanylyltransferase that catalyzes the activation of (2S)-2-phospholactate (2-PL) as (2S)-lactyl-2-diphospho-5'-guanosine, via the condensation of 2-PL with GTP. It is involved in the biosynthesis of coenzyme F420, a hydride carrier cofactor.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="5">
            <text>(2S)-2-phospholactate + GTP + H(+) = (2S)-lactyl-2-diphospho-5'-guanosine + diphosphate</text>
            <dbReference type="Rhea" id="RHEA:63424"/>
            <dbReference type="ChEBI" id="CHEBI:15378"/>
            <dbReference type="ChEBI" id="CHEBI:33019"/>
            <dbReference type="ChEBI" id="CHEBI:37565"/>
            <dbReference type="ChEBI" id="CHEBI:59435"/>
            <dbReference type="ChEBI" id="CHEBI:59906"/>
            <dbReference type="EC" id="2.7.7.68"/>
        </reaction>
    </comment>
    <comment type="pathway">
        <text evidence="5">Cofactor biosynthesis; coenzyme F420 biosynthesis.</text>
    </comment>
    <comment type="subunit">
        <text evidence="5">Homodimer.</text>
    </comment>
    <comment type="similarity">
        <text evidence="5">Belongs to the CofC family.</text>
    </comment>
    <comment type="caution">
        <text evidence="6">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="2.7.7.68" evidence="5"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96792.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589698.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W099"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01162"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.5"/>
    <dbReference type="OrthoDB" id="11179at2157"/>
    <dbReference type="UniPathway" id="UPA00071"/>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005525">
        <property type="term" value="F:GTP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-KW"/>
    </dbReference>
    <dbReference type="GO" id="GO:0043814">
        <property type="term" value="F:phospholactate guanylyltransferase activity"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-EC"/>
    </dbReference>
    <dbReference type="GO" id="GO:0052645">
        <property type="term" value="P:F420-0 metabolic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.90.550.10">
        <property type="entry name" value="Spore Coat Polysaccharide Biosynthesis Protein SpsA, Chain A"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_02114">
        <property type="entry name" value="CofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR002835">
        <property type="entry name" value="CofC"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR029044">
        <property type="entry name" value="Nucleotide-diphossugar_trans"/>
    </dbReference>
    <dbReference type="NCBIfam" id="TIGR03552">
        <property type="entry name" value="F420_cofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40392">
        <property type="entry name" value="2-PHOSPHO-L-LACTATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR40392:SF1">
        <property type="entry name" value="2-PHOSPHO-L-LACTATE GUANYLYLTRANSFERASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF01983">
        <property type="entry name" value="CofC"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF53448">
        <property type="entry name" value="Nucleotide-diphospho-sugar transferases"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="4 5" id="KW-0342">GTP-binding</keyword>
    <keyword evidence="3 5" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="2 5" id="KW-0548">Nucleotidyltransferase</keyword>
    <keyword evidence="7" id="KW-1185">Reference proteome</keyword>
    <keyword evidence="1 5" id="KW-0808">Transferase</keyword>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00022679"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00022695"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00023134"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_02114"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="6">
        <source>
            <dbReference type="EMBL" id="ENN96792.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="7">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="217" mass="24989" checksum="43D5363E89FD20FC" modified="2013-06-26" version="1">MKALIPVSPLDSLKTRLSEFLNKEERKFLLFNMLKDIFKALEGLEVYVISKDNEILDFVKGLGGRVIEEKYKGLNNAIKQGFNEIRGNEVLIIPADIPLIKKRHINEIMILSKNYDAIISPSRGGGTNLLYLKDKRSIEVRYEGFSFLKHIDEFKKHGLKYYIYDSFYLSIDVNTVEDLGEIFIHGEGTETKRYLMDLNIKVLPKHSSAGRFEIVRG</sequence>
</entry>
<entry dataset="TrEMBL" created="2013-06-26" modified="2025-10-08" version="48" xmlns="https://uniprot.org/uniprot">
    <accession>N6W0C6</accession>
    <name>N6W0C6_9EURY</name>
    <protein>
        <recommendedName>
            <fullName evidence="10">5-formaminoimidazole-4-carboxamide-1-(beta)-D-ribofuranosyl 5'-monophosphate synthetase</fullName>
            <ecNumber evidence="10">6.3.4.23</ecNumber>
        </recommendedName>
        <alternativeName>
            <fullName evidence="10">5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase</fullName>
        </alternativeName>
    </protein>
    <gene>
        <name evidence="10 13" type="primary">purP</name>
        <name evidence="13" type="ORF">J422_00230</name>
    </gene>
    <organism evidence="13 14">
        <name type="scientific">Methanocaldococcus villosus KIN24-T80</name>
        <dbReference type="NCBI Taxonomy" id="1069083"/>
        <lineage>
            <taxon>Archaea</taxon>
            <taxon>Methanobacteriati</taxon>
            <taxon>Methanobacteriota</taxon>
            <taxon>Methanomada group</taxon>
            <taxon>Methanococci</taxon>
            <taxon>Methanococcales</taxon>
            <taxon>Methanocaldococcaceae</taxon>
            <taxon>Methanocaldococcus</taxon>
        </lineage>
    </organism>
    <reference evidence="13 14" key="1">
        <citation type="journal article" date="2013" name="Genome Announc." volume="1" first="E00481" last="13">
            <title>Draft Genome Sequence of a Highly Flagellated, Fast-Swimming Archaeon, Methanocaldococcus villosus Strain KIN24-T80 (DSM 22612).</title>
            <authorList>
                <person name="Thennarasu S."/>
                <person name="Polireddy D."/>
                <person name="Antony A."/>
                <person name="Yada M.R."/>
                <person name="Algarawi S."/>
                <person name="Sivakumar N."/>
            </authorList>
            <dbReference type="PubMed" id="23846275"/>
        </citation>
        <scope>NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]</scope>
        <source>
            <strain evidence="13 14">KIN24-T80</strain>
        </source>
    </reference>
    <comment type="function">
        <text evidence="10">Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates.</text>
    </comment>
    <comment type="catalytic activity">
        <reaction evidence="10">
            <text>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + formate + ATP = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide + ADP + phosphate</text>
            <dbReference type="Rhea" id="RHEA:24836"/>
            <dbReference type="ChEBI" id="CHEBI:15740"/>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
            <dbReference type="ChEBI" id="CHEBI:43474"/>
            <dbReference type="ChEBI" id="CHEBI:58467"/>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
            <dbReference type="ChEBI" id="CHEBI:456216"/>
            <dbReference type="EC" id="6.3.4.23"/>
        </reaction>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="2">
            <name>Mg(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:18420"/>
        </cofactor>
    </comment>
    <comment type="cofactor">
        <cofactor evidence="1">
            <name>Mn(2+)</name>
            <dbReference type="ChEBI" id="CHEBI:29035"/>
        </cofactor>
    </comment>
    <comment type="pathway">
        <text evidence="10">Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (formate route): step 1/1.</text>
    </comment>
    <comment type="similarity">
        <text evidence="10">Belongs to the phosphohexose mutase family.</text>
    </comment>
    <comment type="caution">
        <text evidence="13">The sequence shown here is derived from an EMBL/GenBank/DDBJ whole genome shotgun (WGS) entry which is preliminary data.</text>
    </comment>
    <dbReference type="EC" id="6.3.4.23" evidence="10"/>
    <dbReference type="EMBL" id="APMM01000001">
        <property type="protein sequence ID" value="ENN96827.1"/>
        <property type="molecule type" value="Genomic_DNA"/>
    </dbReference>
    <dbReference type="RefSeq" id="WP_004589733.1">
        <property type="nucleotide sequence ID" value="NZ_APMM01000001.1"/>
    </dbReference>
    <dbReference type="AlphaFoldDB" id="N6W0C6"/>
    <dbReference type="STRING" id="1069083.GCA_000371805_01123"/>
    <dbReference type="PATRIC" id="fig|1069083.5.peg.46"/>
    <dbReference type="OrthoDB" id="98133at2157"/>
    <dbReference type="UniPathway" id="UPA00074">
        <property type="reaction ID" value="UER00134"/>
    </dbReference>
    <dbReference type="Proteomes" id="UP000053695">
        <property type="component" value="Unassembled WGS sequence"/>
    </dbReference>
    <dbReference type="GO" id="GO:0005524">
        <property type="term" value="F:ATP binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0016879">
        <property type="term" value="F:ligase activity, forming carbon-nitrogen bonds"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="GO" id="GO:0000287">
        <property type="term" value="F:magnesium ion binding"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="InterPro"/>
    </dbReference>
    <dbReference type="GO" id="GO:0006189">
        <property type="term" value="P:'de novo' IMP biosynthetic process"/>
        <property type="evidence" value="ECO:0007669"/>
        <property type="project" value="UniProtKB-UniRule"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.40.50.20">
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.1490.20">
        <property type="entry name" value="ATP-grasp fold, A domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Gene3D" id="3.30.470.20">
        <property type="entry name" value="ATP-grasp fold, B domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="HAMAP" id="MF_01163">
        <property type="entry name" value="IMP_biosynth_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR011761">
        <property type="entry name" value="ATP-grasp"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR013815">
        <property type="entry name" value="ATP_grasp_subdomain_1"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR023656">
        <property type="entry name" value="IMP_biosynth_PurP"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR009720">
        <property type="entry name" value="IMP_biosynth_PurP_C"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR010672">
        <property type="entry name" value="IMP_biosynth_PurP_N"/>
    </dbReference>
    <dbReference type="InterPro" id="IPR016185">
        <property type="entry name" value="PreATP-grasp_dom_sf"/>
    </dbReference>
    <dbReference type="NCBIfam" id="NF009780">
        <property type="entry name" value="PRK13278.1-5"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147:SF2">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PANTHER" id="PTHR38147">
        <property type="entry name" value="5-FORMAMINOIMIDAZOLE-4-CARBOXAMIDE-1-(BETA)-D-RIBOFURANOSYL 5'-MONOPHOSPHATE SYNTHETASE-RELATED"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06849">
        <property type="entry name" value="DUF1246"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="Pfam" id="PF06973">
        <property type="entry name" value="DUF1297"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PIRSF" id="PIRSF004602">
        <property type="entry name" value="ATPgrasp_PurP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF56059">
        <property type="entry name" value="Glutathione synthetase ATP-binding domain-like"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="SUPFAM" id="SSF52440">
        <property type="entry name" value="PreATP-grasp domain"/>
        <property type="match status" value="1"/>
    </dbReference>
    <dbReference type="PROSITE" id="PS50975">
        <property type="entry name" value="ATP_GRASP"/>
        <property type="match status" value="1"/>
    </dbReference>
    <proteinExistence type="inferred from homology"/>
    <keyword evidence="7 10 11" id="KW-0067">ATP-binding</keyword>
    <keyword evidence="3 10 13" id="KW-0436">Ligase</keyword>
    <keyword evidence="8" id="KW-0460">Magnesium</keyword>
    <keyword evidence="9" id="KW-0464">Manganese</keyword>
    <keyword evidence="4" id="KW-0479">Metal-binding</keyword>
    <keyword evidence="5 10 11" id="KW-0547">Nucleotide-binding</keyword>
    <keyword evidence="6 10" id="KW-0658">Purine biosynthesis</keyword>
    <keyword evidence="14" id="KW-1185">Reference proteome</keyword>
    <feature type="domain" description="ATP-grasp" evidence="12">
        <location>
            <begin position="124"/>
            <end position="348"/>
        </location>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="27"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="94"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="230"/>
        </location>
        <ligand>
            <name>ATP</name>
            <dbReference type="ChEBI" id="CHEBI:30616"/>
        </ligand>
    </feature>
    <feature type="binding site" evidence="10">
        <location>
            <position position="258"/>
        </location>
        <ligand>
            <name>5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide</name>
            <dbReference type="ChEBI" id="CHEBI:58475"/>
        </ligand>
    </feature>
    <evidence type="ECO:0000256" key="1">
        <source>
            <dbReference type="ARBA" id="ARBA00001936"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="2">
        <source>
            <dbReference type="ARBA" id="ARBA00001946"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="3">
        <source>
            <dbReference type="ARBA" id="ARBA00022598"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="4">
        <source>
            <dbReference type="ARBA" id="ARBA00022723"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="5">
        <source>
            <dbReference type="ARBA" id="ARBA00022741"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="6">
        <source>
            <dbReference type="ARBA" id="ARBA00022755"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="7">
        <source>
            <dbReference type="ARBA" id="ARBA00022840"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="8">
        <source>
            <dbReference type="ARBA" id="ARBA00022842"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="9">
        <source>
            <dbReference type="ARBA" id="ARBA00023211"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="10">
        <source>
            <dbReference type="HAMAP-Rule" id="MF_01163"/>
        </source>
    </evidence>
    <evidence type="ECO:0000256" key="11">
        <source>
            <dbReference type="PROSITE-ProRule" id="PRU00409"/>
        </source>
    </evidence>
    <evidence type="ECO:0000259" key="12">
        <source>
            <dbReference type="PROSITE" id="PS50975"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="13">
        <source>
            <dbReference type="EMBL" id="ENN96827.1"/>
        </source>
    </evidence>
    <evidence type="ECO:0000313" key="14">
        <source>
            <dbReference type="Proteomes" id="UP000053695"/>
        </source>
    </evidence>
    <sequence length="361" mass="41069" checksum="418EF061F463ED33" modified="2013-06-26" version="1">MIKKDEIIEIFNEYNKDEITIATLGSHTALHILKGSKLEGFSTVCITVKGRDIPYKRFRVADKFIYVNDFSEIKDEEVQEKLREMNSIIIPHGSFIAYCGLDNIEKNFYVPMFGNRMILRWEAERDLERKLLEEAGIRVPKKFEDPNDIDRCVIVKFPGARGGKGYFIASSKDEFYKKANELIDKGILTDEDLKKAHIEEYIIGTNFCFHYFYSPLKNEVELLGIDKRYESNIDGLIRIPAKDQLESNINPSYVITGNIPVVIRESLLPKVFDMGDKLVEASKSIVPPGIIGPFCLQSLCTDSLEIVVFEMSARIDGGSNSFMNGSPYSFLYNGEPLSMGQRIAREIKLALSLNMIEKIIS</sequence>
</entry>
<copyright>
Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms Distributed under the Creative Commons Attribution (CC BY 4.0) License
</copyright>
</uniprot>